研究者情報

マスター

アカウント（マスター）

• 氏名

  小野寺　智洋(オノデラ　トモヒロ), オノデラ　トモヒロ

所属（マスター）

• 医学研究院　専門医学系部門　機能再生医学分野

所属（マスター）

• 医学研究院　専門医学系部門　機能再生医学分野

独自項目

syllabus

• 2021, 基本医学研究, Master's Thesis Research in Medical Sciences, 修士課程, 医学院, 整形外科学的研究，筋骨格系研究，脊髄末梢神経研究 Orthopedic research, Musculoskeletal research, Spinal cord and peripheral nerve research
• 2021, 基本医学総論, Basic Principles of Medicine, 修士課程, 医学院, 運動器，形態学，骨軟骨代謝学，免疫学，運動学，生体力学 Musculoskeletal system, Bone and Cartilage metabolism, Morphology, Immunology, Kinematics, Biomechanics
• 2021, 基本医学総論, Basic Principles of Medicine, 修士課程, 医学院, 脊椎再建医学、組織工学、分子生物学 Spinal reconstruction, Bioengineering, molecular biology
• 2021, ソフトマター医工学特論, Soft Matter Medical Engineering, 修士課程, 生命科学院, 再生医学、臨床医学、入門
• 2021, ソフトマター科学概論, Soft Matter Science, 修士課程, 生命科学院, （生命医薬科学概論） 有機合成化学，天然物化学，神経，免疫，蛋白質，脂質，遺伝子解析，RNA，バイオイメージング解析，薬剤吸収，薬物送達，痛み，立体構造 （生命融合科学概論/ソフトマター科学概論） 生命融合科学，生命情報分子科学，生命物質科学，細胞機能科学，生命機能制御科学，ソフトマター科学，ソフトマター材料科学，ソフトマター生命分子科学，ソフトマター生体物理学，ソフトマター医科学，SDGs （生命システム科学概論） 細胞増殖，細胞極性，細胞分化，形態形成，遺伝子発現，植物免疫，神経回路，動物行動学，能科学，生殖機構，発生，内分泌，ホルモン，オムニバス，現代生命科学，知的財産
• 2021, 生命融合科学概論, Transdisciplinary Life Science, 修士課程, 生命科学院, （生命医薬科学概論） 有機合成化学，天然物化学，神経，免疫，蛋白質，脂質，遺伝子解析，RNA，バイオイメージング解析，薬剤吸収，薬物送達，痛み，立体構造 （生命融合科学概論/ソフトマター科学概論） 生命融合科学，生命情報分子科学，生命物質科学，細胞機能科学，生命機能制御科学，ソフトマター科学，ソフトマター材料科学，ソフトマター生命分子科学，ソフトマター生体物理学，ソフトマター医科学，SDGs （生命システム科学概論） 細胞増殖，細胞極性，細胞分化，形態形成，遺伝子発現，植物免疫，神経回路，動物行動学，能科学，生殖機構，発生，内分泌，ホルモン，オムニバス，現代生命科学，知的財産
• 2021, 医学総論, Principles of Medicine, 博士後期課程, 医学院, 運動器，病態，治療法，臨床研究 Musculoskeletal system, etiology, treatment, clinical research
• 2021, 医学総論, Principles of Medicine, 博士後期課程, 医学院, 運動器，形態学、骨軟骨代謝学，免疫学、運動学、生体材料工学、生体力学，再生工学 Musculoskeletal system, bone and cartilage metabolism, immunology, kinematics, pathology, anatomy, biomechanics, bioengineering
• 2021, 基盤医学研究, Dissertation Research in Medical Sciences, 博士後期課程, 医学院, 運動器，形態学、骨・軟骨代謝学，免疫学、運動学、生体力学，生体工学，生体材料 Musculoskeletal organs, Morphology, Bone and Cartilage metabolism, Immunology, Kinematics, Biomechanics, Biomaterials
• 2021, 臨床医学研究, Dissertation Research in Clinical Medicine, 博士後期課程, 医学院, 脊椎再建医学、組織工学、骨代謝、分子生物学 Spinal reconstruction, tissue engineering, bone metabolism, molecular biology.

researchmap

プロフィール情報

学位

• 医学博士（北海道大学大学院医学研究科）

プロフィール情報

• プロフィール

  略歴：2007年 北海道大学大学院医学研究科博士課程 修了，2006年より米国NIH National Institute of Dental and Craniofacial Research Visiting fellowとしてこの研究を行う．2009年より北海道大学大学院医学研究科 助教．
  研究テーマ：足の外科，膝関節外科，先天性小児疾患，器官形態形成．
  現在は，整形外科医としての臨床業務と基礎研究との二足の草鞋を履かせてもらっています．両立は非常に困難ですが，基礎・臨床を両方とも経験している立場として，少しでも互いの橋渡しができるようがんばっていきたいと思います．

• 姓

  小野寺, オノデラ

• 名

  智洋, トモヒロ

• ID各種

  201201043828545099

対象リソース

http://kaken.nii.ac.jp/d/r/70547174.ja.html

業績リスト

研究キーワード

• 再生医療   バイオマテリアル   整形外科・関節外科   

研究分野

• ライフサイエンス / 生体医工学
• ライフサイエンス / 生体材料学
• ライフサイエンス / 整形外科学

経歴

• 2024年04月 - 現在 北海道大学大学院医学研究院 専門医学系部門 機能再生医学分野 整形外科学教室 准教授
• 2016年04月 - 現在 北海道大学病院 整形外科 講師
• 2011年04月 - 2016年03月 北海道大学 医学(系)研究科(研究院) 助教

委員歴

• 2015年08月 - 現在   北海道整形災害外科学会   評議員
• 2014年09月 - 現在   日本足の外科学会   評議員
• 2014年09月 - 現在   東日本整形災害外科学会   評議員

受賞

• 2019年 日本足の外科学会 日本足の外科学会日韓トラベリングフェローシップ
  
• 2018年09月 日本医師会 日本医師会医学研究奨励賞
   

  受賞者: 小野寺智洋
• 2018年09月 日本整形外科学会 日整会Travelling Fellowship（Korean Orthopaedic Association Annual Congress）
   

  受賞者: 小野寺智洋
• 2013年 整形災害外科学研究助成財団 アルケア奨励賞
   

  受賞者: 小野寺智洋
• 2012年 北海道整形災害外科学会 学術奨励賞
   

  受賞者: 小野寺智洋
• 2011年09月 北海道大学大学院医学研究科 第30回 高桑榮松奨学基金奨励賞
   

  受賞者: 小野寺智洋
• 2011年 北海道大学医学部同窓会 フラテ研究奨励賞
   

  受賞者: 小野寺智洋
• 2007年09月 日本学術振興会 JSPS Postdoctoral Fellowship for Research Abroad
   

  受賞者: 小野寺智洋
• 2007年09月 NIH The Fellows Award for Research Excellence
   

  受賞者: 小野寺智洋

論文

• CCR7 depletion alleviates bony growth imbalance following physeal injury in mice.

  WooYoung Kim, Yuko Sakai, Masatake Matsuoka, Yoshiaki Hosokawa, Ryuichi Fukuda, Kentaro Homan, Tomohiro Onodera, Norimasa Iwasaki

  Scientific reports 14 1 24891 - 24891 2024年10月22日 

  Growth plates are the frequent sites of skeletal injury in children, leading to skeletal growth imbalances. Chemokines, including the receptor CCR7, play a crucial role in stem cell recruitment and cartilage homeostasis, with previous studies linking CCR7 to osteoarthritis progression. However, its role in growth plate cartilage remains unclear. We analyzed the role of CCR7 in the physeal cartilage repair process in mice model. Physeal injury was created in the proximal tibia in 3-week-old C57BL/6 mice (WT) and CCR7-knockout mice (CCR7-/-). Tibial length was measured macroscopically and sections of the physeal injury were analyzed histologically and immunohistochemically. Height and bone volume of the tibial growth plate and bone mineral density (BMD) of the subchondral area were measured by micro-CT. Mesenchymal stem cells (MSCs) were harvested and gene expression after osteogenic differentiation was analyzed using qRT-PCR. At 1, 3 and 5 weeks postoperatively, injured tibiae of CCR7-/- mice were less shortened than those of WT mice. Bone volume of the physeal bridge was significantly lower in CCR7-/- mice than in WT mice. In contrast, BMD of the subchondral area was comparable between CCR-/- and WT mice, and between sham and operated tibiae. In osteogenic differentiation, CCR7-/- mice showed significantly lowered expression of osteogenic markers such as Osterix, Runx2 and Type X collagen. We demonstrated CCR7 depletion in mice inhibited physeal bridge formation and ameliorated growth imbalances after physeal injury.
• Evaluating the Alignment of Artificial Intelligence-Generated Recommendations With Clinical Guidelines Focused on Soft Tissue Tumors.

  Masatake Matsuoka, Tomohiro Onodera, Ryuichi Fukuda, Koji Iwasaki, Masanari Hamasaki, Taku Ebata, Yoshiaki Hosokawa, Eiji Kondo, Norimasa Iwasaki

  Journal of surgical oncology 2024年09月05日 

  BACKGROUND: The integration of artificial intelligence (AI), particularly, in oncology, has significantly shifted the paradigms of medical diagnostics and treatment planning. However, the utility of AI, specifically OpenAI's ChatGPT, in soft tissue sarcoma treatment, remains unclear. METHODS: We evaluated ChatGPT's alignment with the Japanese Orthopaedic Association (JOA) clinical practice guidelines on the management of soft tissue tumors 2020. Twenty-two clinical questions (CQs) were formulated to encompass various aspects of sarcoma diagnosis, treatment, and management. ChatGPT's responses were classified into "Complete Alignment," "Partial Alignment," or "Nonalignment" based on the recommendation and strength of evidence. RESULTS: ChatGPT demonstrated an 86% alignment rate with the JOA guidelines. The AI provided two instances of complete alignment and 17 instances of partial alignment, indicating a strong capability to match guideline criteria for most questions. However, three discrepancies were identified in areas concerning the treatment of atypical lipomatous tumors, perioperative chemotherapy for synovial sarcoma, and treatment strategies for elderly patients with malignant soft tissue tumors. Reassessment with guideline input led to some adjustments, revealing both the potential and limitations of AI in complex sarcoma care. CONCLUSION: Our study demonstrates that AI, specifically ChatGPT, can align with clinical guidelines for soft tissue sarcoma treatment. It also underscores the need for continuous refinement and cautious integration of AI in medical decision-making, particularly in the context of treatment for soft tissue sarcoma.
• Higher association of articular surface destruction with rheumatoid forefoot deformity arising from dislocation of the metatarsophalangeal joints in the lateral column.

  Yuki Suzuki, Tomohiro Onodera, Koji Iwasaki, Masatake Matsuoka, Masanari Hamasaki, Eiji Kondo, Norimasa Iwasaki

  Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association 2024年08月14日 

  BACKGROUND: To evaluate the relationship between the pattern and severity of metatarsophalangeal (MTP) joint dislocation in the lesser toes and severity of joint destruction in rheumatoid forefoot deformities. METHODS: Participants comprised of 13 patients (16 feet) who underwent resection arthroplasty of the metatarsal head of the lesser toes for rheumatoid arthritis of the MTP joints. Correlations between preoperative radiographic findings and histological grades second to fifth metatarsal heads taken intraoperatively were analyzed. RESULTS: In 62 metatarsal heads, complete dislocation of the MTP joint in the lesser toes significantly resulted in severe joint destruction compared to mild or moderate dislocation (P < 0.05). The proportion of severe cartilage damage in MTP joints with complete dislocation was 100 % in the 5th MTP joint, 83.3 % in the 4th MTP joint, and 58.3 % in the 2nd and 3rd MTP joints. Moreover, complete dislocation of the MTP joints in the lateral column showed the most severe joint destruction compared to that in the medial column (P = 0.03). CONCLUSION: Complete dislocation of the MTP joint in the lateral column is related to joint destruction in rheumatoid forefoot deformities.
• Amputation surgery associated with shortened survival in patients with localized extremity bone sarcoma.

  Nayuhito Yanagisawa, Masatake Matsuoka, Tomohiro Onodera, Koji Iwasaki, Masanari Hamasaki, Taku Ebata, Yoshiaki Hosokawa, Eiji Kondo, Norimasa Iwasaki

  Journal of orthopaedics 54 124 - 130 2024年08月 

  BACKGROUND: This study assesses survival rates among patients with localized extremity bone sarcoma who have undergone amputation, pinpointing subpopulations that are disproportionately affected by amputation-related survival disparities. METHODS: Examination of data was conducted using the Surveillance, Epidemiology, and End Results (SEER) program, analyzing records of 3765 patients diagnosed with localized extremity bone sarcoma between 2000 and 2019. Of these, 857 received amputations (Amputation cohort), and 2908 underwent limb-sparing surgeries. Propensity score matching, considering demographic and clinical features, was utilized to ensure a fair comparison. RESULTS: Following propensity score matching, the study focused on 1714 cases. The Amputation cohort was observed to have significantly poorer survival rates (Cancer-Specific Survival [CSS]: Hazard Ratio [HR] = 1.28, 95% Confidence Interval [CI]: 1.05-1.55; Overall Survival [OS]: HR = 1.37, 95% CI: 1.15-1.63). Subsequent subgroup analysis indicated that individuals with tumors exceeding 8 cm in size or those located in the lower limbs were notably at a higher risk of shortened survival (for tumors >8 cm - CSS: HR = 1.32, 95% CI: 1.02-1.71; OS: HR = 1.39, 95% CI: 1.09-1.76; for lower limb tumors - CSS: HR = 1.25, 95% CI: 1.01-1.54; OS: HR = 1.33, 95% CI: 1.11-1.61). CONCLUSIONS: Our findings demonstrate that patients diagnosed with localized extremity bone sarcoma undergoing amputation exhibit lower survival rates, especially in cases involving tumors of greater size or those situated in the lower limbs. In patient groups where amputation is inevitable, careful follow-up is required after surgical intervention.
• Depletion of b-series ganglioside prevents limb length discrepancy after growth plate injury.

  Yoshiaki Hosokawa, Masatake Matsuoka, Yuko Sakai, Ryuichi Fukuda, Keizumi Matsugasaki, Kentaro Homan, Jun-Ichi Furukawa, Tomohiro Onodera, Norimasa Iwasaki

  BMC musculoskeletal disorders 25 1 565 - 565 2024年07月20日 

  INTRODUCTION: Growth plate damage in long bones often results in progressive skeletal growth imbalance and deformity, leading to significant physical problems. Gangliosides, key glycosphingolipids in cartilage, are notably abundant in articular cartilage and regulate chondrocyte homeostasis. This suggests their significant roles in regulating growth plate cartilage repair. METHODS: Chondrocytes from 3 to 5 day-old C57BL/6 mice underwent glycoblotting and mass spectrometry. Based on the results of the glycoblotting analysis, we employed GD3 synthase knockout mice (GD3-/-), which lack b-series gangliosides. In 3-week-old mice, physeal injuries were induced in the left tibiae, with right tibiae sham operated. Tibiae were analyzed at 5 weeks postoperatively for length and micro-CT for growth plate height and bone volume at injury sites. Tibial shortening ratio and bone mineral density were measured by micro-CT. RESULTS: Glycoblotting analysis indicated that b-series gangliosides were the most prevalent in physeal chondrocytes among ganglioside series. At 3 weeks, GD3-/- exhibited reduced tibial shortening (14.7 ± 0.2 mm) compared to WT (15.0 ± 0.1 mm, P = 0.03). By 5 weeks, the tibial lengths in GD3-/- (16.0 ± 0.4 mm) closely aligned with sham-operated lengths (P = 0.70). Micro-CT showed delayed physeal bridge formation in GD3-/-, with bone volume measuring 168.9 ± 5.8 HU at 3 weeks (WT: 180.2 ± 3.2 HU, P = 0.09), but normalizing by 5 weeks. CONCLUSION: This study highlights that GD3 synthase knockout mice inhibit physeal bridge formation after growth plate injury, proposing a new non-invasive approach for treating skeletal growth disorders.
• Radiation therapy improves survival in elderly patients with localized extremity soft tissue sarcoma.

  Masatake Matsuoka, Tomohiro Onodera, Koji Iwasaki, Masanari Hamasaki, Taku Ebata, Yoshiaki Hosokawa, Ryuichi Fukuda, Eiji Kondo, Norimasa Iwasaki

  Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association 2024年07月15日 

  BACKGROUND: This study aims to evaluate the efficacy of radiation therapy in enhancing survival outcomes among elderly patients diagnosed with localized extremity soft tissue sarcomas (STSs). Furthermore, it seeks to explore whether the survival benefits conferred by radiation therapy differ according to tumor characteristics and treatment modalities. METHODS: We conducted a retrospective analysis using the Surveillance, Epidemiology, and End Results (SEER) database to identify patients aged 80 years and older diagnosed with localized extremity STSs. The study assessed 1498 eligible patients, analyzing cancer-specific survival (CSS) and overall survival (OS) through Kaplan-Meier survival analysis and Cox proportional hazards modeling. Propensity score matching was employed to adjust for potential confounders. RESULTS: Among the analyzed patients, 773 patients underwent radiation therapy (Radiation group), while 725 patients either did not receive radiation therapy or it was unknown if they had (No-Radiation group). The 5-year OS rate was 40% in the radiation group compared to 38% in the no-radiation group. After propensity score matching, radiation therapy was associated with a significant improvement in OS (P = 0.005, HR = 0.8, 95% CI 0.7-0.9). Subgroup analyses indicated that patients undergoing primary tumor resection benefited most from radiation therapy in terms of OS. CONCLUSION: Radiation therapy is associated with improved overall survival in elderly patients with localized extremity STSs. These findings suggest that radiation therapy should be considered as a key component of the treatment strategy for this patient population, taking into account individual patient characteristics and comorbidities.
• Paradoxical Change in Subchondral Bone Density in the Medial Compartment of the Proximal Tibial Articular Surface After High Tibial Osteotomy: A Detailed Subchondral Bone Density Analysis.

  Yuki Fujie, Koji Iwasaki, Masanari Hamasaki, Yuki Suzuki, Masatake Matsuoka, Tomohiro Onodera, Eiji Kondo, Norimasa Iwasaki

  The American journal of sports medicine 52 9 3635465241256100 - 3635465241256100 2024年06月20日 

  BACKGROUND: High tibial osteotomy (HTO) aims to realign the varus knee to alleviate stress in the medial compartment. However, detailed information on the impact of HTO on stress distribution across the tibiofemoral joint surface still needs to be completely elucidated. PURPOSE/HYPOTHESIS: The present study aimed to analyze the subchondral bone density distribution to validate the alignment threshold causing paradoxical changes. We hypothesized that there would be a paradoxical stress change in the medial compartment beyond a specific threshold for lower limb realignment after HTO. STUDY DESIGN: Case series; Level of evidence, 4. METHODS: A retrospective clinical study of 32 knees from 30 patients who underwent medial opening-wedge HTO between 2015 and 2019 was conducted at Hokkaido University Hospital. The subchondral bone density across the tibiofemoral joint was analyzed using computed tomography-osteoabsorptiometry before and after HTO. The high-density area (HDA) within the medial and lateral compartments and their subregions, which were quartered in the coronal plane, was specifically examined. RESULTS: The hip-knee-ankle angle, medial proximal tibial angle (MPTA), joint line obliquity (JLO), and joint line convergence angle significantly changed after HTO (P < .01). The HDA of the medial compartment to the total HDA ratio decreased from 83% to 77%. Paradoxically, the HDA in the most central subregion of the medial compartment increased from 24% to 30%. There were significant differences between MPTA and JLO in patients with and without paradoxical changes in the HDA. MPTA and JLO cutoff values causing paradoxical changes in the HDA were 94° and 4°, respectively. CONCLUSION: There was a paradoxical stress increase in the M4 region at the medial compartment associated with the MPTA and JLO beyond specific thresholds. Therefore, surgical planning should be cautiously performed to prevent overcorrection, which can lead to adverse stress distribution changes.
• Elucidation of the association between additional distal femoral resection and extension angle improvement following the actual surgical steps with the Robot-TKA system.

  Ryosuke Hishimura, Koji Iwasaki, Yuki Suzuki, Masatake Matsuoka, Tomohiro Onodera, Eiji Kondo, Norimasa Iwasaki

  The Knee 49 36 - 44 2024年06月04日 

  BACKGROUND: This study investigates the association between additional distal femoral resection and improved flexion contracture in total knee arthroplasty (TKA) with a robot-assisted system. Flexion contracture is a common issue in patients with knee osteoarthritis, which causes postoperative complications and functional limitations. This study aims to evaluate the effectiveness of additional bone resection in flexion contracture correction and knee extension angle improvement after the actual surgical steps. METHODS: The study included 11 patients who underwent posterior-stabilized (PS)-type TKA with a robot-assisted system. The surgical technique consisted of precise bone resection and range of motion evaluation using a navigation system. A precut technique was used to facilitate posterior access and remove osteophytes that cause the contracture. The amount of additional distal femoral resection was determined based on the thickness of the insert trial required for achieving full extension. RESULTS: The flexion contracture correction angle and the amount of additional distal femoral resection demonstrated a linear relationship. An average of 2.0° with the standard error (SE) of 0.6° improvement in flexion contracture was observed per 1.0 mm of additional bone resection. The postoperative evaluation demonstrated a significant improvement in knee extension angle, thereby reducing the contracture degree. CONCLUSION: This study was the first to perform the additional distal femoral resection on the living knee, which closely replicates the actual surgical steps. The current study revealed that an additional 1.0 mm of distal femoral resection in PS-type TKA improves knee extension angle by 2.0° (SE 0.6°) within an additional resection range of 1.0 mm to 3.3 mm.
• Atypical lipomatous tumor/well differentiated liposarcomaの脱分化率の検討 米国SEERデータベースを用いた研究

  松岡 正剛, 小野寺 智洋, 福田 龍一, 岩崎 浩司, 濱崎 雅成, 江畑 拓, 細川 吉暁, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 98 6 S1500 - S1500 (公社)日本整形外科学会 2024年06月
• 軟部腫瘍診療ガイドラインを用いた対話型オープンAIによる診療支援ツールとしての可能性

  松岡 正剛, 小野寺 智洋, 福田 龍一, 岩崎 浩司, 濱崎 雅成, 江畑 拓, 細川 吉暁, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 98 6 S1533 - S1533 (公社)日本整形外科学会 2024年06月
• Ultra-rare sarcomaの臨床的特徴 米国SEERデータベースを用いた研究

  福田 龍一, 松岡 正剛, 小野寺 智洋, 岩崎 浩司, 江畑 拓, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 98 6 S1617 - S1617 (公社)日本整形外科学会 2024年06月
• 四肢原発非円形細胞肉腫において治療開始までの期間が生存に与える影響 米国SEERデータベースを用いた研究

  高橋 桜子, 松岡 正剛, 小野寺 智洋, 岩崎 浩司, 濱崎 雅成, 江畑 拓, 細川 吉暁, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 98 6 S1619 - S1619 (公社)日本整形外科学会 2024年06月
• 総腓骨神経麻痺を契機に診断された膝外側半月板嚢腫の1例

  奥村 眞子, 松岡 正剛, 小野寺 智洋, 岩崎 浩司, 宮石 陸, 細川 吉暁, 濱崎 雅成, 江畑 拓, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 98 6 S1664 - S1664 (公社)日本整形外科学会 2024年06月
• ヒト変形性膝関節症血清における新規N型糖鎖バイオマーカーの網羅的解析とその臨床的意義

  小野寺 智洋, 宝満 健太郎, 花松 久寿, 古川 潤一, 岩崎 浩司, 松岡 正剛, 濱崎 雅成, 江畑 拓, 細川 吉暁, 近藤 英司, 岩崎 倫政

  日本関節病学会誌 43 2 175 - 175 (一社)日本関節病学会 2024年06月
• 同位体顕微鏡による関節軟骨内水動態の組織学的評価

  松ヶ崎 圭純, 小野寺 智洋, 宝満 健太郎, 亀田 浩之, 工藤 與亮, 坂本 直哉, 細川 吉暁, 松岡 正剛, 福田 龍一, 坂井 裕子, 劉 越, 岩崎 倫政

  日本関節病学会誌 43 2 176 - 176 (一社)日本関節病学会 2024年06月
• Analysis of foot-originating malignant bone tumors: Epidemiology, characteristics, and survival outcomes.

  Masatake Matsuoka, Tomohiro Onodera, Koji Iwasaki, Masanari Hamasaki, Taku Ebata, Yoshiaki Hosokawa, Ryuichi Fukuda, Eiji Kondo, Norimasa Iwasaki

  Foot and ankle surgery : official journal of the European Society of Foot and Ankle Surgeons 2024年05月29日 

  BACKGROUND: The study examines the characteristics and outcomes of foot-originating malignant bone tumors via Surveillance Epidemiology and End Results (SEER) database analysis. METHODS: A retrospective review of 14,695 malignant bone tumor cases from 2000 to 2019 was conducted. RESULTS: Of the eligible cases, 147 (2.3 %) were foot-origin tumors, typically smaller and more commonly treated with surgery than those in other locations. These tumors were more frequently treated with surgical resection, with a higher proportion undergoing amputation. In contrast, foot-origin tumors were less often managed with chemotherapy and radiation. Foot-origin tumors exhibited higher survival rates compared to non-foot-origin tumors as shown in univariate analysis, although multivariate analysis did not reflect significant differences. CONCLUSION: Foot-originating malignant bone tumors tend to be smaller and are frequently surgically treated, correlating with favorable survival outcomes. These findings point to early detection as a potential factor in the improved survival rates, not necessarily the tumor's origin.
• Glycosphingolipids in Osteoarthritis and Cartilage-Regeneration Therapy: Mechanisms and Therapeutic Prospects Based on a Narrative Review of the Literature.

  Kentaro Homan, Tomohiro Onodera, Masatake Matsuoka, Norimasa Iwasaki

  International journal of molecular sciences 25 9 2024年04月30日 

  Glycosphingolipids (GSLs), a subtype of glycolipids containing sphingosine, are critical components of vertebrate plasma membranes, playing a pivotal role in cellular signaling and interactions. In human articular cartilage in osteoarthritis (OA), GSL expression is known notably to decrease. This review focuses on the roles of gangliosides, a specific type of GSL, in cartilage degeneration and regeneration, emphasizing their regulatory function in signal transduction. The expression of gangliosides, whether endogenous or augmented exogenously, is regulated at the enzymatic level, targeting specific glycosyltransferases. This regulation has significant implications for the composition of cell-surface gangliosides and their impact on signal transduction in chondrocytes and progenitor cells. Different levels of ganglioside expression can influence signaling pathways in various ways, potentially affecting cell properties, including malignancy. Moreover, gene manipulations against gangliosides have been shown to regulate cartilage metabolisms and chondrocyte differentiation in vivo and in vitro. This review highlights the potential of targeting gangliosides in the development of therapeutic strategies for osteoarthritis and cartilage injury and addresses promising directions for future research and treatment.
• The alterations in nerve growth factor concentration in plasma and synovial fluid before and after total knee arthroplasty.

  Tomohiro Onodera, Koji Iwasaki, Masatake Matsuoka, Yasuhide Morioka, Shinji Matsubara, Eiji Kondo, Norimasa Iwasaki

  Scientific reports 14 1 8943 - 8943 2024年04月18日 

  Total knee arthroplasty (TKA) is an effective procedure for pain relief; however, the emergence of postsurgical pain remains a concern. In this study, we investigated the production of nerve growth factor (NGF) and mediators that affect NGF production and their function in the synovial fluid and plasma after TKA. This study included 19 patients (20 knees) who had rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and knee osteoarthritis (OA) who underwent TKA, categorized into OA and non-OA groups. The levels of NGF, inflammatory cytokines, and lipid mediators were analyzed before and after surgery. The intraoperative synovial fluid NGF concentration was more than seven times higher in the non-OA group than in the OA group. The intra-articular NGF levels increased significantly by more than threefold postoperatively in the OA group but not in the non-OA group. Moreover, the levels of inflammatory cytokines and lipid mediators were increased in the synovial fluid of both groups. The intra-articular cytokines or NGF concentrations positively correlated with postoperative pain. Targeted NGF control has the potential to alleviate postsurgical pain in TKA, especially in patients with OA, emphasizing the importance of understanding NGF dynamics under different knee conditions.
• Surgical Resection of Primary Tumor for Bone Metastatic Breast Cancer Patients at Initial Presentation.

  Nayu Kitsuya, Masatake Matsuoka, Tomohiro Onodera, Isao Yokota, Koji Iwasaki, Yuki Suzuki, Masanari Hamasaki, Eiji Kondo, Norimasa Iwasaki

  Anticancer research 44 4 1591 - 1601 2024年04月 

  BACKGROUND/AIM: The purpose of this study was to investigate whether primary tumor resection in patients with bone metastatic breast cancer has an impact on survival using the Surveillance, Epidemiology, and End Results database, considering subtype classification. PATIENTS AND METHODS: We included all female patients with bone metastatic breast cancer at initial presentation between 2010 and 2016 with known hormone receptor (HR) and human epidermal growth factor receptor 2 (HER2) statuses. Cases showing unknown cause of death or unknown HR/HER2 status were excluded. Survival analysis was performed using Cox proportional hazards modeling to calculate hazard ratios (HZR). RESULTS: Of the 13,450 patients included in this study, 2,073 patients were HR+/HER2+, 8,597 patients were HR+/HER2-, 797 patients were HR-/HER2+, and 1,182 patients were HR-/HER2- (triple-negative). Five-year overall survival (OS) rate was 34.5% in HR+/HER2+, 26.0% in HR+/HER2-, 29.2% in HR-/HER2+ and 8.0% in triple-negative. Triple-negative patients showed the worsen OS [HR+/HER2+: HZR=2.1, 95% confidence interval (CI)=1.9-2.3; HR+/HER2-: HZR= 2.4, 95%CI=2.2-2.6; HR-/HER2+: HZR=1.5, 95%CI=1.3-1.6]. After excluding patients who died within six months, primary tumor resection prolonged survival in each subtype classification except HR-/HER2+. CONCLUSION: Patients with triple-negative bone metastatic breast cancer showed unfavorable survival. Primary tumor resection prolonged survival in each subtype except for HR-/HER2+.
• Bone Density Distribution Pattern in the Lateral Wall of the Femoral Intercondylar Notch: Implications for the Direct Insertion of the Femoral ACL Attachment.

  Yutaro Sugawara, Koji Iwasaki, Yuki Suzuki, Ryosuke Hishimura, Shinji Matsubara, Masatake Matsuoka, Tomohiro Onodera, Eiji Kondo, Norimasa Iwasaki

  Orthopaedic journal of sports medicine 12 4 23259671241236807 - 23259671241236807 2024年04月 

  BACKGROUND: The ideal position of the femoral bone tunnel in the anterior cruciate ligament (ACL) is controversial. The functional importance of the ACL fiber varies depending on where it is attached to the femur. Functionally important fibers can cause high mechanical stress on the bone, and the Wolff law predicts that bone mineral density will increase at high mechanical stress sites. PURPOSE/HYPOTHESIS: The purpose of this study was to use computed tomography imaging to determine the distribution pattern of bone density in the lateral intercondylar wall. It was hypothesized that the high-density area (HDA) of the lateral intercondylar wall would reflect the functional insertion of the ACL as reported in previous anatomic studies. STUDY DESIGN: Descriptive epidemiology study. METHODS: Data from 39 knees without ACL injuries were retrospectively collected. The HDA of the lateral intercondylar wall was defined as the region containing the top 10% of the radiodensity values. The shape of the HDA was approximated as an ellipse, and the quadrant method was used to determine the center of the ellipse. The association between the ratio of the minor axis to the major axis of the ellipse and background characteristics was investigated. RESULTS: According to the quadrant method, the center of the HDA ellipse was 33.6% in the deep-shallow direction and 23.4% in the high-low direction. The center of the ellipse was comparable to the anatomic center of the ACL footprint, as previously reported. The ratio of the minor axis to the major axis of the ellipse was 0.58 (95% CI, 0.54-0.62). There was a significant negative correlation between the ratio of the minor axis to the major axis of the HDA ellipse and the posterior tibial slope (r = -0.38, P = .02). CONCLUSION: The center of the HDA ellipse was found to be similar to the anatomic center of the ACL footprint. Considering the mechanical stress responses in bone, the HDA of the lateral intercondylar wall has the potential to represent the ACL insertion, especially functional insertion.
• Articular cartilage corefucosylation regulates tissue resilience in osteoarthritis.

  Kentaro Homan, Tomohiro Onodera, Hisatoshi Hanamatsu, Jun-Ichi Furukawa, Daisuke Momma, Masatake Matsuoka, Norimasa Iwasaki

  eLife 12 2024年03月11日 

  This study aimed to investigate the glycan structural changes that occur before histological degeneration in osteoarthritis (OA) and to determine the mechanism by which these glycan conformational changes affect cartilage degeneration. An OA model was established in rabbits using mannosidase injection, which reduced high-mannose type N-glycans and led to cartilage degeneration. Further analysis of glycome in human OA cartilage identified specific corefucosylated N-glycan expression patterns. Inhibition of N-glycan corefucosylation in mice resulted in unrecoverable cartilage degeneration, while cartilage-specific blocking of corefucosylation led to accelerated development of aging-associated and instability-induced OA models. We conclude that α1,6 fucosyltransferase is required postnatally to prevent preosteoarthritic deterioration of articular cartilage. These findings provide a novel definition of early OA and identify glyco-phenotypes of OA cartilage, which may distinguish individuals at higher risk of progression.
• 遠隔転移を有する原発性悪性骨腫瘍において原発巣切除は生存を改善するか? 米国SEERデータベースを用いた研究

  福田 龍一, 松岡 正剛, 小野寺 智洋, 岩崎 浩司, 江畑 拓, 濱崎 雅成, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 98 2 S94 - S94 (公社)日本整形外科学会 2024年03月
• 足部に発生した原発性悪性骨腫瘍の生存解析 SEER databaseを用いた研究

  松岡 正剛, 小野寺 智洋, 岩崎 浩司, 濱崎 雅成, 江畑 拓, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 98 2 S277 - S277 (公社)日本整形外科学会 2024年03月
• 80歳以上高齢者に発生した四肢発生軟部肉腫における放射線照射

  松岡 正剛, 小野寺 智洋, 岩崎 浩司, 濱崎 雅成, 江畑 拓, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 98 2 S415 - S415 (公社)日本整形外科学会 2024年03月
• 女性乳がんサブタイプ分類が骨転移症例における生存へ与える影響 米国SEERデータベースを用いた研究

  松岡 正剛, 木津谷 菜悠, 小野寺 智洋, 横田 勲, 岩崎 浩司, 濱崎 雅成, 江畑 拓, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 98 2 S417 - S417 (公社)日本整形外科学会 2024年03月
• コラーゲン膜を用いた自家培養軟骨移植の臨床成績 骨膜被覆との比較

  甲斐原 拓真, 近藤 英司, 濱崎 雅成, 江畑 拓, 原 健人, 岩崎 浩司, 松岡 正剛, 小野寺 智洋, 門間 太輔, 岩崎 倫政

  日本整形外科学会雑誌 98 2 S448 - S448 (公社)日本整形外科学会 2024年03月
• 逆V字型高位脛骨骨切り術後のスポーツ復帰

  江畑 拓, 近藤 英司, 濱崎 雅成, 原 健人, 岩崎 浩司, 松岡 正剛, 小野寺 智洋, 岩崎 倫政

  日本整形外科学会雑誌 98 3 S818 - S818 (公社)日本整形外科学会 2024年03月
• 内側閉鎖式大腿骨遠位内反骨切り術が膝蓋大腿関節の応力分布に与える影響 CT osteoabsorptiometry法を用いた検討

  濱崎 雅成, 近藤 英司, 江畑 拓, 松岡 正剛, 岩崎 浩司, 小野寺 智洋, 井上 雅之, 安田 和則, 八木 知徳, 岩崎 倫政

  日本整形外科学会雑誌 98 3 S911 - S911 (公社)日本整形外科学会 2024年03月
• 高位脛骨骨切り術前後の膝関節矢状面における応力分布の変化についての検討

  藤江 裕貴, 岩崎 浩司, 江畑 拓, 濱崎 雅成, 松岡 正剛, 小野寺 智洋, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 98 3 S913 - S913 (公社)日本整形外科学会 2024年03月
• 膝軟骨下骨脆弱性骨折の骨髄浮腫面積が高位脛骨骨切り術後の臨床成績に与える影響

  原 健人, 近藤 英司, 薮内 康史, 江畑 拓, 濱崎 雅成, 松岡 正剛, 岩崎 浩司, 小野寺 智洋, 八木 知徳, 岩崎 倫政

  日本整形外科学会雑誌 98 3 S959 - S959 (公社)日本整形外科学会 2024年03月
• 脛骨関節面の軟骨下骨の骨密度分布に与える半月板の影響の検討に適した半月板評価法の検討

  岩崎 浩司, 佐藤 知哉, 江畑 拓, 濱崎 雅成, 原 健人, 松岡 正剛, 小野寺 智洋, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 98 3 S1097 - S1097 (公社)日本整形外科学会 2024年03月
• The Influence of Patello-Femoral Overstuffing After Modular Unlinked Bicompartmental Knee Arthroplasty (BiKA) for Medial Tibio-Femoral and Patello-Femoral Osteoarthritis of the Knee.

  Rikiya Baba, Yasumitsu Ohkoshi, Tatsunori Maeda, Ko Suzuki, Akane Iwadate, Koji Iwasaki, Tomohiro Onodera, Eiji Kondo, Norimasa Iwasaki

  The Journal of arthroplasty 39 3 638 - 644 2024年03月 

  BACKGROUND: The factors affecting results after bicompartmental knee arthroplasty (BiKA) have not been fully elucidated. This major ligament-preserving procedure may be more susceptible to overstuffing of the patello-femoral (PF) joint than the major ligament-sacrificing total knee arthroplasty. Currently, we investigated the effect of PF overstuffing after BiKA on its clinical outcome. METHODS: There were 71 patients (74 knees) who underwent modular unlinked BiKA at our clinic who had a follow-up of 5 to 9 years. Final follow-up results were assessed by evaluating knee range of motion, the 2011 Knee Society Score (2011KSS), Japanese Knee Osteoarthritis Measure, and radiological findings. The degree of postoperative PF overstuffing was evaluated by computed tomography and magnetic resonance images for 55 knees, and the correlation between the degree of overstuffing and postoperative clinical results were examined. RESULTS: Overall clinical results improved significantly after surgery without any revision cases. The X-ray measurements showed the improved coronal alignments and the appropriate implant installation angles. Higher degree of postoperative PF overstuffing caused by insufficient amount of osteotomy on the anterior surface of the femur correlated with worse postoperative total 2011KSS at 2 years after surgery (Spearman's rank correlation coefficient (rs) = -0.387, P = .004), as opposed to no correlation at the time of the final follow-up (Spearman's rank correlation coefficient = 0.068, P = .623). CONCLUSION: Modular unlinked BiKA provided patients with a high level of satisfaction and functional improvement over 5 to 9 years postoperatively. However, because PF overstuffing affects initial patient satisfaction, the amount of osteotomy should be determined carefully during the surgery.
• Simultaneous medial closing wedge distal femoral varus osteotomy and double-bundle anterior cruciate ligament reconstruction in the symptomatic femoral valgus deformity: A case report.

  Masatake Matsuoka, Eiji Kondo, Koji Iwasaki, Tomohiro Onodera, Ryuichi Nakamura, Hiroshi Nakayama, Takenori Akiyama, Daisuke Momma, Norimasa Iwasaki

  Joint diseases and related surgery 35 2 422 - 432 2024年02月13日 

  Distal femoral varus osteotomy (DFVO) is a widely recognized surgical procedure used to address valgus malalignment in patients with knee joint disorders. However, it still remains unclear whether anterior cruciate ligament (ACL) reconstruction can be performed in a single procedure along with DFVO. Herein, we present a 73-year-old female patient who developed lateral osteoarthritis of the knee with valgus alignment due to chronic ACL deficiency following a twisting injury during skiing. She was physiologically very active, and strongly demanded to return to sports. We performed a combined procedure involving a medial closing wedge DFVO using an anatomical locking plate, along with double-bundle ACL reconstruction. The postoperative radiograph confirmed successful correction of knee alignment, specifically achieving varus alignment with precise conformance of the anatomical plate to the medial contour of the distal femur following the osteotomy. The patient resumed her previous sports activities without experiencing knee pain. The operated knee demonstrated restored anterior stability, as indicated by negative Lachman test results, and regained full range of motion. Both the Knee Injury and Osteoarthritis Outcome Score and the 2011 Knee Society score demonstrated continuous postoperative improvements over the three-year follow-up period, indicating positive functional outcomes and joint preservation. To the best of our knowledge, this is the first case of medial closing wedge DFVO with anatomic double-bundle ACL reconstruction in the symptomatic femoral valgus deformity with chronic ACL deficiency in the literature.
• Bone metastatic cancer of unknown primary at initial presentation.

  Masatake Matsuoka, Tomohiro Onodera, Isao Yokota, Koji Iwasaki, Yuki Suzuki, Hamasaki Masanari, Eiji Kondo, Norimasa Iwasaki

  Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 26 2 461 - 467 2024年02月 

  INTRODUCTION: Cancer of unknown primary (CUP) is a challenging malignancy. The purpose of this study was to investigate the clinical characteristics and prognosis of bone metastatic CUP using the population-based Surveillance, Epidemiology, and End Results (SEER) database. METHODS: From the SEER database, we identified 1908 patients with bone metastatic CUP at initial presentation between 2010 and 2018. Histology was subdivided following International Classification of Diseases for Oncology codes as Adenocarcinoma, Squamous cell, Neuroendocrine, or Carcinoma not otherwise specified (NOS). Cox proportional hazard modeling was applied using factors of age, sex, ethnicity, histological subtype, and therapeutic intervention. RESULTS: Among the 1908 patients, histology was Neuroendocrine in 240 patients, Squamous cell in 201 patients, Adenocarcinoma in 810 patients and NOS in 657 patients. In each subtype, patients tended to be predominantly male and white. Chemotherapy was introduced for 28% of patients and radiation for 34% in the entire cohort. Survival in patients with bone metastatic CUP was unfavorable, with a median survival of 2 months. Among the histological subtypes, Adenocarcinoma showed shorter survival than the other groups. In addition, treatment interventions such as chemotherapy and radiation therapy prolonged survival, particularly for Squamous cell, Adenocarcinoma and NOS, but not for Neuroendocrine. DISCUSSION: Bone metastatic CUP showed extremely poor prognosis, but treatment interventions such as chemotherapy and radiation generally offered survival benefits. Further randomized clinical research is needed to confirm the present results.
• Magnetic Resonance Water Tracer Imaging Using 17 O-Labeled Water.

  Hiroyuki Kameda, Naoya Kinota, Daisuke Kato, Takaaki Fujii, Taisuke Harada, Yuji Komaki, Hiroyuki Sugimori, Tomohiro Onodera, Moyoko Tomiyasu, Takayuki Obata, Kohsuke Kudo

  Investigative radiology 59 1 92 - 103 2024年01月01日 

  Magnetic resonance imaging (MRI) is a crucial imaging technique for visualizing water in living organisms. Besides proton MRI, which is widely available and enables direct visualization of intrinsic water distribution and dynamics in various environments, MR-WTI (MR water tracer imaging) using 17 O-labeled water has been developed, benefiting from the many advancements in MRI software and hardware that have substantially improved the signal-to-noise ratio and made possible faster imaging. This cutting-edge technique allows the generation of novel and valuable images for clinical use. This review elucidates the studies related to MRI water tracer techniques centered around 17 O-labeled water, explaining the fundamental principles of imaging and providing clinical application examples. Anticipating continued progress in studies involving isotope-labeled water, this review is expected to contribute to elucidating the pathophysiology of various diseases related to water dynamics abnormalities and establishing novel imaging diagnostic methods for associated diseases.
• Atelocollagen-associated autologous chondrocyte implantation for the repair of large cartilage defects of the knee: Results at three to seven years.

  Takuma Kaibara, Eiji Kondo, Masatake Matsuoka, Koji Iwasaki, Tomohiro Onodera, Keita Sakamoto, Yoshitaka Oda, Zen-Ichi Tanei, Daisuke Momma, Shinya Tanaka, Norimasa Iwasaki

  Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association 29 1 207 - 216 2024年01月 

  BACKGROUND: Recently, various types of engineered autologous chondrocyte implantation (ACI) have been developed. Atelocollagen-associated ACI (A-ACI) is the only ACI procedure covered by Japanese Health Insurance since 2013. The indications of the A-ACI are traumatic cartilage defects and osteochondral dissecans (OCD) for knee joints. PURPOSE: To evaluate midterm clinical results after A-ACI for the treatment for full-thickness cartilage defects of the knee. METHODS: Thirteen consecutive patients who underwent A-ACI between 2014 and 2018 had been prospectively enrolled in this study. There were 11 men and 2 women with a mean age of 34 years at the time of surgery. The causes of the cartilage defect were trauma in 10 knees and OCD in 3 knees. The total number of lesions was 15, which were comprised of the medial femoral condyle in 5 knees, the lateral femoral condyle in 5 knees, and the femoral trochlea in 5 knees. The mean size of the lesion was 5.3 cm2. Each knee was clinically and radiologically evaluated preoperatively and postoperatively. RESULTS: The mean Lysholm score improved significantly from 74.0 points to 94.0 points (p = 0.008) and each subscale in Knee injury and Osteoarthritis Outcome Score improved significantly (p < 0.001) at the mean final follow-up period of 51 months (range, 36-84 months). The magnetic resonance observation of cartilage repair tissue 2.0 score at the mean follow-up of 38 months was significantly higher than that at 2 months postoperatively (p = 0.014). According to the International Cartilage Repair Society (ICRS) grading scale, 3 knees were graded as normal, 3 knees as nearly normal, and 1 knee as severely abnormal in second-look arthroscopic evaluation at a mean of 22 months (range, 8-41 months) after A-ACI. CONCLUSION: The present study showed a significant subjective and objective clinical improvement in the A-ACI for large cartilage defects of the knee at a mean follow-up of 51 months (range, 36-84 months).
• Assessing knee joint biomechanics and trunk posture according to medial osteoarthritis severity.

  Yuki Suzuki, Yasumitsu Ohkoshi, Kensaku Kawakami, Kenta Shimizu, Shuya Chida, Kengo Ukishiro, Tomohiro Onodera, Koji Iwasaki, Tatsunori Maeda, Sho'ji Suzuki, Eiji Kondo, Norimasa Iwasaki

  Scientific reports 13 1 19186 - 19186 2023年11月06日 

  During progression of knee osteoarthritis (OA), gait biomechanics changes three-dimensionally; however, its characteristics and trunk posture according to OA severity remain unknown. The present study investigated three-dimensional knee joint biomechanics and trunk posture according to knee OA severity. Overall, 75 patients (93 knees) with medial knee OA [Kellgren-Lawrence grade ≥ 2, grade 2: 20 patients with 24 knees (mean 60.0 years old); grade 3: 25 with 28 knees (mean 62.0 years old); grade 4: 30 with 41 knees (mean 67.9 years old)] and 14 healthy controls (23 knees, mean 63.6 years old) underwent gait analysis using an optical motion capture system and point cluster technique. In grade 2 knee OA, the relative contribution of the knee adduction moment (KAM) increased significantly (P < 0.05), and that of the knee flexion moment decreased (P < 0.05) prior to significant progression of varus knee deformity. Grade 3 knee OA showed significant exacerbation of varus knee deformity (P < 0.01) and KAM increase (P < 0.001). The maximum knee extension angle decreased (P < 0.05) and trunk flexion increased during gait in grade 4 knee OA (P < 0.001). Our study clarified the kinematics and kinetics of medial knee OA with trunk flexion according to severity. Kinetic conversion occurred in grade 2 knees prior to progression of varus deformities, knee flexion contractures, and sagittal imbalance during gait in patients with severe knee OA.
• Effect of Patient Age on Clinical and Radiological Outcomes After Medial Open-Wedge High Tibial Osteotomy: A Comparative Study With 344 Knees.

  Koji Yabuuchi, Eiji Kondo, Takuma Kaibara, Jun Onodera, Koji Iwasaki, Masatake Matsuoka, Tomohiro Onodera, Norimasa Iwasaki, Tomonori Yagi, Kazunori Yasuda

  Orthopaedic journal of sports medicine 11 10 23259671231200227 - 23259671231200227 2023年10月 

  BACKGROUND: There exists some controversy regarding whether patient age is a predictive factor for outcomes after high tibial osteotomy (HTO). PURPOSE/HYPOTHESIS: The purpose of this study was to evaluate whether patient age affects clinical and radiological outcomes after medial open-wedge HTO (OWHTO) in a large population with a wider age range than previous studies. It was hypothesized that there would be no differences in outcomes when compared across age-groups. STUDY DESIGN: Cohort study; Level of evidence, 3. METHODS: A retrospective comparative study was conducted using 344 patients (303 knees) who underwent OWHTO from 2009 to 2018. These patients were divided into 3 groups based on age at the time of surgery: ≥55 years (group Y: 76 knees in 57 patients), 56 to 64 years (group M: 129 knees in 120 patients), and ≤65 years (group O: 139 knees in 126 patients). Clinical and radiological evaluations were performed immediately before surgery and at the final follow-up period, at a mean of 5.1 years (range, 3-11 years). Comparisons among the 3 groups were conducted with 1-way analysis of variance for continuous variables. When a significant result was obtained, a post hoc test with Bonferroni correction was conducted for multiple comparisons. RESULTS: In clinical evaluations, there were no significant differences among the 3 groups either preoperatively or postoperatively concerning the Japanese Orthopaedic Association score, the Lysholm score, or the Knee injury and Osteoarthritis Outcome Score (KOOS), with the exception of the preoperative KOOS Symptoms subscale, which was significantly higher in group Y versus group O (48.9 ± 18.7 vs 58.7 ± 15.4, respectively; P = .011). The Tegner activity score was significantly different among the groups, both preoperatively and postoperatively (P < .001 for both). There was no significant difference in the occurrence of complications or the survival rate at final follow-up among the 3 groups. CONCLUSION: The study findings suggest that patient age does not affect clinical and radiological outcomes after OWHTO.
• Targeted next-generation sequencing for detection of PIK3CA mutations in archival tissues from patients with Klippel-Trenaunay syndrome in an Asian population : List the full names and institutional addresses for all authors.

  Yuki Sasaki, Kosuke Ishikawa, Kanako C Hatanaka, Yumiko Oyamada, Yusuke Sakuhara, Tadashi Shimizu, Tatsuro Saito, Naoki Murao, Tomohiro Onodera, Takahiro Miura, Taku Maeda, Emi Funayama, Yutaka Hatanaka, Yuhei Yamamoto, Satoru Sasaki

  Orphanet journal of rare diseases 18 1 270 - 270 2023年09月04日 

  BACKGROUND: Klippel-Trenaunay syndrome (KTS) is a rare slow-flow combined vascular malformation with limb hypertrophy. KTS is thought to lie on the PIK3CA-related overgrowth spectrum, but reports are limited. PIK3CA encodes p110α, a catalytic subunit of phosphatidylinositol 3-kinase (PI3K) that plays an essential role in the PI3K/AKT/mammalian target of rapamycin (mTOR) signaling pathway. We aimed to demonstrate the clinical utility of targeted next-generation sequencing (NGS) in identifying PIK3CA mosaicism in archival formalin-fixed paraffin-embedded (FFPE) tissues from patients with KTS. RESULTS: Participants were 9 female and 5 male patients with KTS diagnosed as capillaro-venous malformation (CVM) or capillaro-lymphatico-venous malformation (CLVM). Median age at resection was 14 years (range, 5-57 years). Median archival period before DNA extraction from FFPE tissues was 5.4 years (range, 3-7 years). NGS-based sequencing of PIK3CA achieved an amplicon mean coverage of 119,000x. PIK3CA missense mutations were found in 12 of 14 patients (85.7%; 6/8 CVM and 6/6 CLVM), with 8 patients showing the hotspot variants E542K, E545K, H1047R, and H1047L. The non-hotspot PIK3CA variants C420R, Q546K, and Q546R were identified in 4 patients. Overall, the mean variant allele frequency for identified PIK3CA variants was 6.9% (range, 1.6-17.4%). All patients with geographic capillary malformation, histopathological lymphatic malformation or macrodactyly of the foot had PIK3CA variants. No genotype-phenotype association between hotspot and non-hotspot PIK3CA variants was found. Histologically, the vessels and adipose tissues of the lesions showed phosphorylation of the proteins in the PI3K/AKT/mTOR signaling pathway, including p-AKT, p-mTOR, and p-4EBP1. CONCLUSIONS: The PI3K/AKT/mTOR pathway in mesenchymal tissues was activated in patients with KTS. Amplicon-based targeted NGS could identify low-level mosaicism from low-input DNA extracted from FFPE tissues, potentially providing a diagnostic option for personalized medicine with inhibitors of the PI3K/AKT/mTOR signaling pathway.
• アテロコラーゲンを用いた自家培養軟骨移植後に軟骨下骨嚢胞を生じた1例

  甲斐原 拓真, 近藤 英司, 濱崎 雅成, 鈴木 裕貴, 岩崎 浩司, 松岡 正剛, 小野寺 智洋, 種井 善一, 田中 伸哉, 岩崎 倫政

  東日本整形災害外科学会雑誌 35 3 361 - 361 東日本整形災害外科学会 2023年08月
• Single-step ultra-purified alginate gel implantation in patients with knee chondral defects.

  Tomohiro Onodera, Daisuke Momma, Masatake Matsuoka, Eiji Kondo, Koji Suzuki, Masayuki Inoue, Masaki Higano, Norimasa Iwasaki

  The bone & joint journal 105-B 8 880 - 887 2023年08月01日 

  AIMS: Implantation of ultra-purified alginate (UPAL) gel is safe and effective in animal osteochondral defect models. This study aimed to examine the applicability of UPAL gel implantation to acellular therapy in humans with cartilage injury. METHODS: A total of 12 patients (12 knees) with symptomatic, post-traumatic, full-thickness cartilage lesions (1.0 to 4.0 cm2) were included in this study. UPAL gel was implanted into chondral defects after performing bone marrow stimulation technique, and assessed for up to three years postoperatively. The primary outcomes were the feasibility and safety of the procedure. The secondary outcomes were self-assessed clinical scores, arthroscopic scores, tissue biopsies, and MRI-based estimations. RESULTS: No obvious adverse events related to UPAL gel implantation were observed. Self-assessed clinical scores, including pain, symptoms, activities of daily living, sports activity, and quality of life, were improved significantly at three years after surgery. Defect filling was confirmed using second-look arthroscopy at 72 weeks. Significantly improved MRI scores were observed from 12 to 144 weeks postoperatively. Histological examination of biopsy specimens obtained at 72 weeks after implantation revealed an extracellular matrix rich in glycosaminoglycan and type II collagen in the reparative tissue. Histological assessment yielded a mean overall International Cartilage Regeneration & Joint Preservation Society II score of 69.1 points (SD 10.4; 50 to 80). CONCLUSION: This study provides evidence supporting the safety of acellular UPAL gel implantation in facilitating cartilage repair. Despite being a single-arm study, it demonstrated the efficacy of UPAL gel implantation, suggesting it is an easy-to-use, one-step method of cartilage tissue repair circumventing the need to harvest donor cells.
• アテロコラーゲンを用いた自家培養軟骨移植後に軟骨下骨嚢胞を生じた1例

  甲斐原 拓真, 近藤 英司, 濱崎 雅成, 鈴木 裕貴, 岩崎 浩司, 松岡 正剛, 小野寺 智洋, 種井 善一, 田中 伸哉, 岩崎 倫政

  東日本整形災害外科学会雑誌 35 3 361 - 361 東日本整形災害外科学会 2023年08月
• Noncanonical Pyroptosis Triggered by Macrophage-Derived Extracellular Vesicles in Chondrocytes Leading to Cartilage Catabolism in Osteoarthritis.

  Taku Ebata, Mohamad Alaa Terkawi, Keita Kitahara, Shunichi Yokota, Junki Shiota, Yoshio Nishida, Gen Matsumae, Hend Alhasan, Masanari Hamasaki, Kazutoshi Hontani, Tomohiro Shimizu, Daisuke Takahashi, Tsutomu Endo, Tomohiro Onodera, Ken Kadoya, Norimasa Iwasaki

  Arthritis & rheumatology (Hoboken, N.J.) 75 8 1358 - 1369 2023年08月 

  OBJECTIVES: The severity of osteoarthritis and cartilage degeneration are highly correlated with the development of synovitis, which is mediated by the activity of inflammatory macrophages. A better understanding of intercellular communication between inflammatory macrophages and chondrocytes should aid in the discovery of novel therapeutic targets. Here, we explored the pathological role of inflammatory macrophage extracellular vesicles in cartilage degeneration. METHODS: Macrophages were stimulated by treatment with bacterial lipopolysaccharides to mimic the state of inflammatory macrophages and the resulting extracellular vesicles were harvested for chondrocyte stimulation in vitro and intraarticular injection in a mouse model. The stimulated chondrocytes were further subjected to RNA-seq analysis and other functional assays. The action of caspase-11 was disrupted in vitro using a specific siRNA or wedelolactone, and in experimental OA-murine models by the intraarticular injection of wedelolactone. RESULTS: Stimulated chondrocytes exhibited a significant elevation in the expression of chondrocyte catabolic factors. Consistent with these results, RNA-seq analyses of stimulated chondrocytes indicated that upregulated genes are mainly categorized into apoptotic process and TNF-signaling pathway which suggests the induction of apoptotic process. Moreover, these chondrocytes exhibited a significant elevation in the expression of pyroptosis-related molecules that were correlated with the expression of chondrocyte catabolic factors. The disruption of caspase-11 significantly alleviated pyroptotic and catabolic processes in stimulated chondrocytes and the pathological changes in collagenase-and joint instability-induced OA models. CONCLUSIONS: Our results provide a new insight into the pathological mechanisms of OA and suggest that non-canonical pyroptosis in chondrocytes represents an attractive therapeutic target for future treatment.
• Symptomatic bilateral complete discoid medial menisci of the knee in a child: A case report.

  Yuki Suzuki, Eiji Kondo, Takuma Kaibara, Masatake Matsuoka, Ryosuke Hishimura, Koji Iwasaki, Tomohiro Onodera, Daisuke Momma, Shinya Tanaka, Norimasa Iwasaki

  Joint diseases and related surgery 34 2 455 - 462 2023年04月26日 

  Bilateral complete discoid medial menisci are extremely rare congenital anatomic variants of the knee. Currently, arthroscopic partial meniscectomy with or without peripheral suture repair is performed for symptomatic discoid meniscus. However, most of the outcomes are short-term. In this article, we present a pediatric case of symptomatic bilateral complete discoid medial menisci and highlight the effectiveness of arthroscopic partial meniscectomy with or without peripheral suture on symptomatic complete discoid medial menisci based on 60-month excellent clinical and functional results.
• Simultaneous and sialic acid linkage-specific N- and O-linked glycan analysis by ester-to-amide derivatization.

  Hisatoshi Hanamatsu, Yoshiaki Miura, Takashi Nishikaze, Ikuko Yokota, Kentaro Homan, Tomohiro Onodera, Yoshihiro Hayakawa, Norimasa Iwasaki, Jun-Ichi Furukawa

  Glycoconjugate journal 40 2 259 - 267 2023年04月 

  Characterization of O-glycans linked to serine or threonine residues in glycoproteins has mostly been achieved using chemical reaction approaches because there are no known O-glycan-specific endoglycosidases. Most O-glycans are modified with sialic acid residues at the non-reducing termini through various linkages. In this study, we developed a novel approach for sialic acid linkage-specific O-linked glycan analysis through lactone-driven ester-to-amide derivatization combined with non-reductive β-elimination in the presence of hydroxylamine. O-glycans released by non-reductive β-elimination were efficiently purified using glycoblotting via chemoselective ligation between carbohydrates and a hydrazide-functionalized polymer, followed by modification of methyl or ethyl ester groups of sialic acid residues on solid-phase. In-solution lactone-driven ester-to-amide derivatization of ethyl-esterified O-glycans was performed, and the resulting sialylated glycan isomers were discriminated by mass spectrometry. In combination with PNGase F digestion, we carried out simultaneous, quantitative, and sialic acid linkage-specific N- and O-linked glycan analyses of a model glycoprotein and human cartilage tissue. This novel glycomic approach will facilitate detailed characterization of biologically relevant sialylated N- and O-glycans on glycoproteins.
• Early Changes in Postural Balance Following Inverted V-Shaped High Tibial Osteotomy in Patients With Knee Osteoarthritis.

  Kento Sabashi, Takeshi Chiba, Koji Iwasaki, Tomohiro Onodera, Eiji Kondo, Norimasa Iwasaki, Harukazu Tohyama

  Journal of applied biomechanics 39 2 124 - 129 2023年04月01日 

  Patients with knee osteoarthritis and varus knee deformity have impaired postural balance, resulting in decreased walking performance and an increased risk of falls. This study aimed to investigate the early changes in the postural balance following inverted V-shaped high tibial osteotomy (HTO). Fifteen patients with medial knee osteoarthritis were recruited. Postural balance was assessed using the center-of-pressure (COP) data during single-leg standing before and 6 weeks after inverted V-shaped HTO. The maximum range, mean velocity, and area of COP movements in the anteroposterior and mediolateral directions were analyzed. Preoperative and postoperative visual analog scale for knee pain was assessed. The maximum range of COP in the mediolateral direction decreased (P = .017), whereas the mean velocity of COP in the anteroposterior direction increased 6 weeks postoperatively (P = .011). The visual analog scale score for knee pain significantly improved at 6 weeks postoperatively (P = .006). Valgus correction with inverted V-shaped HTO resulted in improved postural balance in the mediolateral direction and good short-term clinical outcomes early following surgery. Early rehabilitation after inverted V-shaped HTO should focus on postural balance in the anteroposterior direction.
• Amputation surgery associated with shortened survival in patients with localized soft tissue sarcoma.

  Masatake Matsuoka, Tomohiro Onodera, Isao Yokota, Koji Iwasaki, Ryosuke Hishimura, Yuki Suzuki, Masanari Hamasaki, Akira Iwata, Eiji Kondo, Norimasa Iwasaki

  Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association 29 2 637 - 645 2023年03月15日 

  BACKGROUND: The objectives of this study were to clarify whether localized extremity soft tissue sarcoma (STS) patients who underwent amputation surgery experienced worsened survival and to identify those patients for whom amputation surgery worsened survival. METHODS: Using the Surveillance, Epidemiology, and End Results database, we identified 8897 patients with localized extremity STS between 1983 and 2016. Of these 6431 patients, 733 patients underwent amputation surgery (Amputation group), and 5698 underwent limb-sparing surgery (Limb-sparing group). RESULTS: After adjusting for patient background by propensity score matching, a total of 1346 patients were included. Patients in the Amputation group showed worsened survival (cancer-specific survival (CSS): hazard ratio (HR) = 1.42, 95% confidence interval (CI) 1.15-1.75, overall survival (OS): HR = 1.41, 95%CI 1.20-1.65). In subclass analysis, patients with high-grade STS, spindle cell sarcoma and liposarcoma in the Amputation group showed shortened survival (high-grade-CSS: HR = 1.44, 95%CI 1.16-1.77, OS: HR = 1.38, 95%CI 1.18-1.62; spindle cell sarcoma-CSS: HR = 4.75, 95%CI 1.56-14.4, OS: HR = 2.32, 95%CI 1.45-3.70; liposarcoma-CSS: HR = 2.91, 95%CI 1.54-5.50, OS: HR = 2.32, 95%CI 1.45-3.70). CONCLUSIONS: Survival was shortened in localized extremity STS patients who received amputation surgery.
• 大腿骨顆間外側壁における骨密度分布 前十字靱帯の大腿骨付着部に関する考察

  菅原 悠太郎, 岩崎 浩司, 遠藤 香織, 菱村 亮介, 松原 新史, 松岡 正剛, 門間 太輔, 小野寺 智洋, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 97 2 S193 - S193 (公社)日本整形外科学会 2023年03月
• 変形性膝関節症患者における下腿加速度と膝関節の荷重分布との関係 ウェアラブルセンサーを用いて

  佐橋 健人, 岩崎 浩司, 安倍 雄一郎, 渡邊 謙太郎, 千徳 風真, 千葉 健, 小野寺 智洋, 近藤 英司, 岩崎 倫政, 遠山 晴一

  日本整形外科学会雑誌 97 3 S864 - S864 (公社)日本整形外科学会 2023年03月
• Higher Association of Pelvis-Knee-Ankle Angle Compared With Hip-Knee-Ankle Angle With Knee Adduction Moment and Patient-Reported Outcomes After High Tibial Osteotomy.

  Koji Iwasaki, Yasumitsu Ohkoshi, Yoshiaki Hosokawa, Shuya Chida, Kengo Ukishiro, Kensaku Kawakami, Sho'ji Suzuki, Tatsunori Maeda, Tomohiro Onodera, Eiji Kondo, Norimasa Iwasaki

  The American journal of sports medicine 51 4 977 - 984 2023年03月 

  BACKGROUND: High tibial osteotomy (HTO) reduces the load distribution of the medial compartment by modifying leg alignment. Knee adduction moment (KAM), a surrogate measure of dynamic loading in the knee joint, decreases after HTO. However, leg alignment does not fully account for KAM. PURPOSE: To assess the association between the pelvis-knee-ankle angle (PKA), a novel radiographic parameter reflecting leg alignment and pelvic width, and KAM and patient-reported outcomes after HTO. STUDY DESIGN: Cross sectional study; Level of evidence, 3. METHODS: PKA is the angle between the line from the midpoint of the anterior superior iliac spine to the center of the knee joint and the mechanical axis of the tibia. In this study, 54 patients with medial compartment knee osteoarthritis and varus alignment who underwent 3-dimensional gait analysis preoperatively and 2 years after medial open-wedge HTO were evaluated. The primary outcomes were hip-knee-ankle angle (HKA), PKA, KAM peaks, and Knee Society Score (KSS). Single and multivariate regression analysis including PKA and KAM peaks as well as other demographic and radiologic factors was performed. RESULTS: HKA was weakly correlated with the first peak KAM (r = -0.33; P < .01) and second peak KAM (r = -0.27; P = .01) before HTO, but not significantly correlated after HTO. PKA was moderately correlated with the first peak KAM (r = 0.45; P < .01) and second peak KAM (r = 0.45; P < .01) before HTO and with the first peak KAM (r = 0.51; P < .01) and second peak KAM (r = 0.56; P < .01) after HTO. Multivariate linear regression revealed that postoperative PKA was still associated with the KAM peaks after HTO. Only postoperative PKA was correlated with the KSS satisfaction subscale (r = -0.30; P = .03). CONCLUSION: Although HKA was not correlated with KAM peaks after HTO, PKA was significantly correlated with KAM peaks in patients with varus knee osteoarthritis after HTO.
• Three-stage total knee arthroplasty combined with deformity correction and leg lengthening using Taylor spatial frames and conversion to internal fixation for severe intra- and extra-articular deformities and hypoplasia in a patient with hemophilic knee arthropathy: A case report.

  Akira Fukushima, Koji Iwasaki, Ryousuke Hishimura, Shinji Matsubara, Zenta Joutoku, Masatake Matsuoka, Tomoyuki Endo, Tomohiro Onodera, Eiji Kondo, Norimasa Iwasaki

  The Knee 40 90 - 96 2023年01月 

  BACKGROUND: Hemophilic arthropathy is a cause of severe knee deformity, because chronic synovitis due to repeated hemarthrosis affects the area of the epiphyseal plates in juvenile cases. Total knee arthroplasty (TKA) is the standard treatment for end-stage knee arthropathy. However, it is difficult to perform one-stage TKA in patients with severe intra- and extra-articular deformities. CASE PRESENTATION: We reported a case of hemophilic arthropathy in a 55-year-old male with leg length discrepancy of 4 cm, limited range of knee motion (-40° extension and 85° flexion), intra-articular deformity (medial proximal tibial angle: 69°; mechanical lateral distal femoral angle: 79°), extra-articular deformity at the distal femoral metaphyseal (30° valgus and 45° flexion deformity), and varus malalignment (% mechanical axis: 33%). We planned a three-stage TKA. Firstly, we performed gradual correction and lengthening of the distal femur using Taylor spatial flame. Six months after surgery, we performed conversion surgery from external fixation to internal fixation. Finally, we performed TKA with rotating hinged type implant. Two years after surgery, physical examination showed a normal gait, leg length discrepancy of 2 cm (the right leg was shorter), improvement in the range of knee motion (0° extension and 100° flexion). CONCLUSION: To the best of our knowledge, this presents the first combination of three-stage TKA with correction of femoral deformity and leg lengthening using a Taylor spatial frame and conversion to internal fixation in a patient with hemophilic knee arthropathy and severe intra- and extra-articular deformities.
• Comparison of clinical features between patients with bone and soft tissue fibrosarcomas.

  Masatake Matsuoka, Tomohiro Onodera, Isao Yokota, Koji Iwasaki, Ryosuke Hishimura, Yuki Suzuki, Eiji Kondo, Norimasa Iwasaki

  Journal of surgical oncology 126 7 1299 - 1305 2022年12月 

  BACKGROUND AND OBJECTIVES: Fibrosarcomas predominantly arise in soft tissues, but can also develop in bone. Because of their rarity, whether bone development has an impact on clinical features has not been addressed. METHODS: We included fibrosarcoma patients diagnosed between 1983 and 2016 in the Surveillance, Epidemiology, and End Results database. Differences in clinical features between fibrosarcoma of bone (FS-B) and fibrosarcoma of soft tissue (FS-ST) were investigated. RESULTS: After excluding patients without information regarding cause of death, site of origin, distant tumor or survival, 1443 patients were included. Of those, 98 patients had FS-B. Patients with FS-B were younger, more frequently male, with fibrosarcomas that more often developed in an extremity and were histologically high-grade. In contrast, no difference in potential to metastasize was observed. Survival was almost equal between FS-B and FS-ST (FS-B/FS-ST: cancer-specific survival, hazard ratio [HR]: 1.2, 95% confidence interval [CI]: 0.8-1.7; overall survival, HR: 1.3, 95% CI: 0.9-1.7). CONCLUSIONS: Our results clearly indicated that patient backgrounds differed, such as younger age and greater tendencies to affect males, develop in an extremity and show high-grade tumor in patients with FS-B. In contrast, no differences were observed in distant metastatic potential or survival.
• Establishment of the removal method of undifferentiated induced pluripotent stem cells coexisting with chondrocytes using R-17F antibody.

  Takuji Miyazaki, Hisatoshi Hanamatsu, Tomohiro Onodera, Jun-Ichi Furukawa, Liang Xu, Kentaro Homan, Rikiya Baba, Toshisuke Kawasaki, Norimasa Iwasaki

  Regenerative medicine 17 11 793 - 803 2022年11月 

  Aim: Tumorigenicity of residual undifferentiated induced pluripotent stem cells (iPSCs) is a major concern. The purpose of this study was to investigate the optimal conditions for removal of iPSCs using R-17F antibody, which recognizes specific glycosphingolipids glycans on undifferentiated iPSCs and exhibits selective cytotoxicity to iPSCs. Materials & methods: After adding of R-17F and secondary antibody to co-cultured iPSCs and chondrocytes, residual iPSCs were quantitatively evaluated by iPS specific glycome analysis. Results: Undifferentiated iPSCs were sufficiently removed using R-17F in combination with an equal amount of a secondary antibody. Furthermore, teratomas were not observed upon transplantation of co-cultured cells pretreated under the same conditions into testes of immunodeficient mice. Conclusion: This removal method incorporating R-17F may be useful for regenerative medicine using iPSCs.
• Occurrence Rate of Cyclops Lesion After Anatomic Double-Bundle ACL Reconstruction: Comparison Between Remnant Tissue Preservation and Resection Methods.

  Ryosuke Hishimura, Eiji Kondo, Yuki Suzuki, Masatake Matsuoka, Koji Iwasaki, Tomohiro Onodera, Daisuke Momma, Tomonori Yagi, Kazunori Yasuda, Norimasa Iwasaki

  Orthopaedic journal of sports medicine 10 10 23259671221130688 - 23259671221130688 2022年10月 

  BACKGROUND: The occurrence rate of cyclops lesion after anatomic double-bundle anterior cruciate ligament (ACL) reconstruction with remnant tissue preservation remains unclear. HYPOTHESIS: The study hypotheses were as follows: (1) the occurrence rate of cyclops lesion will be comparable between the remnant-preserving and remnant-resecting ACL reconstruction methods, and (2) there will be no significant differences in clinical outcomes between the remnant-preserving and remnant-resecting procedures. METHODS: This retrospective comparative study involved 177 patients who underwent unilateral anatomic double-bundle ACL reconstruction using hamstring tendon autografts from 2014 to 2018 at our hospital. According to the Crain classification of ACL remnant tissue, 98 patients with remnant types I, II, or III underwent the remnant-preserving procedure (group A), and the remaining 79 patients with remnant type IV underwent the remnant-resecting procedure (group B). All patients underwent second-look arthroscopy. Patients were evaluated according to arthroscopic and clinical results at postoperative 15.2 ± 8.4 months (mean ± SD). Statistical comparisons between groups were made using the paired Student t test, chi-square test, and Fisher exact test. STUDY DESIGN: Cohort study; Level of evidence, 3. RESULTS: At second-look arthroscopy, the incidence of cyclops lesions was significantly higher in group B than in group A (29.1% vs 13.3%; P = .0139). Cyclops lesions were divided into 4 locations: femoral side (type 1), midsubstance (type 2), tibial side (type 3), and anterior (type 4) of the ACL graft. The ratio of the tibial-side cyclops lesion (type 3) was significantly higher in group B than in group A (P = .0354). There were no significant differences in the clinical evaluation scores between the procedures. Side-to-side anterior laxity was significantly less in group A than in group B (0.7 vs 1.6 mm; P = .0035). Concerning postoperative laceration and synovium coverage of the grafts, group A was significantly better than group B (P < .0001). CONCLUSION: In this cohort of patients undergoing double-bundle ACL reconstruction, resection of the ACL remnant was associated with a significantly higher rate of cyclops lesion formation when compared with preservation of the remnant.
• 軟骨細胞パイロトーシス制御による軟骨変性抑制効果

  江畑 拓, 照川 アラー, 横田 隼一, 照川 ヘンド, 松前 元, 清水 智弘, 高橋 大介, 小野寺 智洋, 角家 健, 岩崎 倫政

  日本整形外科学会雑誌 96 8 S1580 - S1580 (公社)日本整形外科学会 2022年09月
• In Reply to the Letter to the Editor Regarding "Does Primary Tumor Resection in Patients with Metastatic Primary Mobile Vertebral Column Sarcoma Improve the Survivals?".

  Masatake Matsuoka, Tomohiro Onodera, Isao Yokota, Norimasa Iwasaki

  World neurosurgery 165 210 - 210 2022年09月
• 変形性膝関節症における歩行中の関節面傾斜の変化 ウェアラブルセンサーによる歩行解析

  岩崎 浩司, 佐橋 健人, 安倍 雄一郎, 千葉 健, 小野寺 智洋, 近藤 英司, 遠山 晴一

  日本整形外科学会雑誌 96 8 S1788 - S1788 (公社)日本整形外科学会 2022年09月
• Liver metastasis in soft tissue sarcoma at initial presentation.

  Yuta Kawae, Masatake Matsuoka, Tomohiro Onodera, Isao Yokota, Koji Iwasaki, Ryosuke Hishimura, Yuki Suzuki, Eiji Kondo, Norimasa Iwasaki

  Journal of surgical oncology 126 6 1074 - 1079 2022年07月06日 

  BACKGROUND AND OBJECTIVES: Noncontrast computed tomography of the chest is the standard imaging modality for cancer staging in patients with soft tissue sarcoma (STS), but appropriate candidates for liver screening have not been clarified. METHODS: We reviewed all patients with STS diagnosed between 2010 and 2018 in the Surveillance, Epidemiology, and End Results database. Incidence of liver metastasis at initial presentation and high incidence of liver metastasis by histological subtype were investigated. In addition, risk factors for liver metastasis were investigated by multiple logistic regression analysis. RESULTS: After excluding patients without information about liver metastasis, cause of death or primary liver tumor, 47 260 patients were included in this study. Of those, 1471 patients (3.2%) showed liver metastasis at initial presentation. The histological subtype showing the highest incidence of liver metastasis was desmoplastic small round cell tumor (30.3%), followed by malignant hemangioendothelioma (11.5%) and angiomyoliposarcoma (10.5%). Deep-rooted tumor, location in the body wall, retroperitoneum, or thorax/peritoneum, high histological grade, and higher T stage were associated with higher incidences of liver metastasis at initial presentation. CONCLUSION: Risk factors for liver metastasis were deep location, trunk development, larger tumor size, high histological grade, and specific histological subtypes.
• Effect of the Inverted V-Shaped Osteotomy on Patellofemoral Joint and Tibial Morphometry as Compared With the Medial Opening Wedge High Tibial Osteotomy.

  Eiji Kondo, Koji Yabuuchi, Zenta Joutoku, Shinji Matsubara, Koji Iwasaki, Masatake Matsuoka, Tomohiro Onodera, Daisuke Momma, Masayuki Inoue, Tomonori Yagi, Norimasa Iwasaki, Kazunori Yasuda

  The American journal of sports medicine 50 9 2439 - 2452 2022年07月 

  BACKGROUND: Recent studies have reported that medial opening wedge (OW) high tibial osteotomy (HTO) induces patella baja, resulting in degenerative changes in the patellofemoral joint. We have developed an inverted V-shaped (iV) HTO, which is classified as a neutral wedge osteotomy. HYPOTHESES: The study hypotheses were as follows: (1) patellar height, posterior tibial slope, and tibial length will not change between pre- and postoperative evaluations after iV-HTO; (2) the lateral shift ratio of the patella and the distance between the tibial tubercle and the trochlear groove may be significantly decreased after iV-HTO. STUDY DESIGN: Cohort study; Level of evidence, 3. METHODS: A total of 191 patients (220 knees) who underwent HTO for medial osteoarthritis were enrolled retrospectively in this study: 107 knees underwent OW-HTO and 113 knees underwent iV-HTO. Clinical and radiological evaluations were performed before and at least 3 years after surgery. RESULTS: Postoperatively, the mean Caton-Deschamps ratio was significantly decreased (P < .0001) from 0.95 to 0.79 in the OW group, while there were no significant changes in the iV group. The mean posterior tibial slope was significantly increased (P < .0001) from 8.5° to 10.5° in the OW group, while there were no significant differences in the iV group. Although the entire leg length was significantly increased (P < .0003) in both groups after HTO, there were no significant differences in tibial length between the pre- and postoperative periods in the iV group. Regarding the congruity of the patellofemoral joint, the mean lateral shift ratio did not significantly change in the OW group, whereas it was significantly decreased (P = .0012) from 11.5% to 8.8% in the iV group. The mean tibial tubercle-trochlear groove distance was significantly decreased (P < .0001) from 12.8 to 9.7 mm in the iV group, while it was significantly increased in the OW group (P < .0001). Concerning the clinical outcome, the Japanese Orthopaedic Association (JOA) and Lysholm knee scores at final follow-up (OW vs iV: JOA, 91.2 vs 90.1; Lysholm, 92.5 vs 89.0) were significantly increased (P < .0001) as compared with the preoperative values (OW vs iV: JOA, 68.3 vs 66.8; Lysholm, 67.9 vs 61.0). CONCLUSION: Patellar height, posterior tibial slope, and tibial length did not change after the iV-HTO, while they were significantly changed after the OW-HTO. Although the preoperative degrees of varus knee and patellofemoral osteoarthritis were more severe in the iV group than the OW group, the iV-HTO led to altered patellofemoral joint congruity.
• Does Primary Tumor Resection in Patients with Metastatic Primary Mobile Vertebral Column Sarcoma Improve Survival?

  Masatake Matsuoka, Tomohiro Onodera, Isao Yokota, Koji Iwasaki, Ryosuke Hishimura, Yuki Suzuki, Akira Iwata, Eiji Kondo, Norimasa Iwasaki

  World neurosurgery 163 e647-e654  2022年04月18日 

  BACKGROUND: Primary mobile vertebral column sarcoma is an exceedingly rare malignancy. Although primary tumor resection has been reported to prolong survival in patients with metastatic bone sarcoma, whether primary tumor resection in patients with advanced primary mobile vertebral column sarcoma is associated with survival remains unclear owing to the rarity of this pathological entity. METHODS: Using the Surveillance, Epidemiology, and End Results database, 182 patients with metastatic primary mobile vertebral column sarcoma were identified between 1983 and 2015. Of the 182 patients enrolled, 101 patients (55%) underwent primary tumor resection (Surgery group) and 81 patients (45%) did not undergo resection (No Surgery group). To account for imbalances in the basic characteristics of patients between groups, propensity score matching was performed. Survival analysis was performed by weighted Cox proportional hazards modeling to calculate hazard ratios. RESULTS: After adjusting for patient background characteristics, 138 patients were included for the analysis (Surgery group: 69 patients; No Surgery group: 69 patients). The Surgery group did not show improved cancer-specific survival (hazard ratio = 0.73, 95% CI 0.49-1.10). Similarly, the Surgery group did not show improved overall survival compared with the No Surgery group (hazard ratio = 0.80, 95% CI 0.55-1.16). CONCLUSIONS: To our knowledge, this is the first study to indicate that surgical resection for advanced primary mobile vertebral column sarcoma does not have a positive impact on survival.
• Intra-articular osteoid osteoma at the elbow mimicking arthritis: a case report

  Ryuichi Fukuda, Masatake Matsuoka, Daisuke Kawamura, Takeshi Endo, Hiromi Kanno-Okada, Atsushi Urita, Yuichiro Matsui, Tomohiro Onodera, Norimasa Iwasaki

  Annals of Joint 7 20 - 20 2022年04月
• Optical coherence tomography evaluation of the spatiotemporal effects of 3D bone marrow stromal cell culture using a bioreactor.

  Jun Yamaguchi, Tomohiro Onodera, Kentaro Homan, Xu Liang, Masatake Matsuoka, Takuji Miyazaki, Hosokawa Yoshiaki, Mitsuru Saito, Norimasa Iwasaki

  Journal of biomedical materials research. Part B, Applied biomaterials 110 8 1853 - 1861 2022年03月09日 

  Performing cell culture in a three-dimensional (3D) environment has various advantages. In cartilage tissue engineering, 3D in vitro cultures utilizing biomaterials and bioreactors can mimic the biological environment. However, the biggest drawback of these 3D culture systems is a limited ability to evaluate 3D cell distribution. Optical coherence tomography (OCT) has recently been used to evaluate 3D cellular morphology and structure in a timely manner. Here, we showed that OCT could be used to visually assess the distribution and the morphology of bone marrow stromal cells under chondrogenic 3D cultivation using alginate gels and rotary culture. In particular, OCT was able to visualize living cells embedded in alginate gels in a non-destructive and 3D manner, as well as quantitatively evaluate cell distribution and spheroid volume. We also found that cells were centralized in rotary culture but peripherally distributed in static culture, while rotary culture enhanced the hypertrophy of marrow stromal cells (MSCs) embedded in alginate gels. Together, our findings demonstrate that OCT can be used to evaluate the spatiotemporal effects of 3D cultivation using alginate gels and rotary culture. Therefore, this method may allow the observation of pre-cultured tissue over time and the optimization of culture conditions for regenerative tissue engineering.
• 関節リウマチ患者の下肢手術数の傾向と併存症発生に関する調査

  糸賀 稜, 清水 智弘, 高橋 大介, 小野寺 智洋, 岩崎 倫政

  日本整形外科学会雑誌 96 2 S334 - S334 (公社)日本整形外科学会 2022年03月
• 関節リウマチ患者の下肢手術数の傾向と併存症に関する調査

  糸賀 稜, 清水 智弘, 小野寺 智洋, 岩崎 倫政

  日本リウマチ学会総会・学術集会プログラム・抄録集 66回 623 - 623 (一社)日本リウマチ学会 2022年03月
• Double-bundle anterior cruciate ligament reconstruction using autologous hamstring tendon hybrid grafts in a patient with hypermobile Ehlers-Danlos Syndrome: A case report.

  Ryosuke Hishimura, Eiji Kondo, Masatake Matsuoka, Koji Iwasaki, Yasuyuki Kawaguchi, Yuki Suzuki, Tomohiro Onodera, Daisuke Momma, Norimasa Iwasaki

  The Knee 35 81 - 86 2022年03月 

  BACKGROUND: Ehlers-Danlos syndrome (EDS) is a connective tissue disorder characterized by skin hyperextensibility, joint hypermobility, and tissue friability. Hypermobile type Ehlers-Danlos syndrome (hEDS) is considered one of the EDS subtypes characterized by generalized joint hypermobility. Although there have been a few case reports which described surgical considerations for anterior cruciate ligament (ACL) reconstructions in patients with other types of EDS, no reports have described those in patients with hEDS. CASE PRESENTATION: We report a case of ACL injury in an 18-year-old male patient with hEDS. The patient was successfully treated with an anatomic double-bundle ACL reconstruction using autologous hamstring tendon hybrid grafts which consist of hamstring tendons connected in a series with commercially available polyester tape. The autogenous tendon portion of the anteromedial and posterolateral bundles were composed of 4 and 2 strands of hamstring tendons, respectively. After 2 weeks of knee joint immobilization, continuous passive motion exercise of the knee joint and partial weight-bearing was allowed. A hinged knee brace was used for a period of 5 months postoperatively. Second-look arthroscopy at 30 months showed that the ACL graft had no laceration and an excellent coverage of the synovium. At 36 months after surgery, the side-to-side differences in the anterior laxity was remarkably improved. The operated knee showed negative Lachman test and had a full range of motion. CONCLUSIONS: To the best of our knowledge, this represents the first report of anatomic double-bundle ACL reconstruction in patients with hEDS and demonstrates excellent clinical and functional outcomes.
• 変形性膝関節症患者における膝および体幹の加速度と膝関節伸展筋力との関係 ウェアラブルセンサーを用いて

  佐橋 健人, 岩崎 浩司, 千葉 健, 小野寺 智洋, 近藤 英司, 岩崎 倫政, 遠山 晴一

  日本整形外科学会雑誌 96 2 S75 - S75 (公社)日本整形外科学会 2022年03月
• 関節リウマチ患者の下肢手術数の傾向と併存症に関する調査

  糸賀 稜, 清水 智弘, 小野寺 智洋, 岩崎 倫政

  日本リウマチ学会総会・学術集会プログラム・抄録集 66回 623 - 623 (一社)日本リウマチ学会 2022年03月
• 手指原発の悪性骨腫瘍における特徴 米国SEERデータベースを用いた研究

  池 翔太, 松岡 正剛, 小野寺 智洋, 横田 勲, 岩崎 浩司, 菱村 亮介, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 96 3 S609 - S609 (公社)日本整形外科学会 2022年03月
• 四肢原発軟部肉腫症例における患肢温存術と切断術の比較 米国SEERデータベースを用いた研究

  松岡 正剛, 小野寺 智洋, 横田 勲, 岩崎 浩司, 菱村 亮介, 鈴木 裕貴, 岩田 玲, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 96 3 S1156 - S1156 (公社)日本整形外科学会 2022年03月
• 初診時遠隔転移を有する椎体発生悪性骨腫瘍において原発巣切除は生存を改善するか 米国SEERデータベースを用いた研究

  松岡 正剛, 小野寺 智洋, 横田 勲, 岩崎 浩司, 菱村 亮介, 鈴木 裕貴, 岩田 玲, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 96 3 S1157 - S1157 (公社)日本整形外科学会 2022年03月
• 関節リウマチ患者の下肢手術数の傾向と併存症発生に関する調査

  糸賀 稜, 清水 智弘, 高橋 大介, 小野寺 智洋, 岩崎 倫政

  日本整形外科学会雑誌 96 2 S334 - S334 (公社)日本整形外科学会 2022年03月
• Primary Malignant Osseous Neoplasms in the Hand.

  Shota Ike, Masatake Matsuoka, Tomohiro Onodera, Isao Yokota, Koji Iwasaki, Ryosuke Hishimura, Yuki Suzuki, Eiji Kondo, Norimasa Iwasaki

  Anticancer research 42 3 1635 - 1640 2022年03月 

  BACKGROUND: Primary malignant osseous neoplasms of the hand are rare malignancies. Comprehensive demographic and survival data regarding primary malignant osseous neoplasms of the hand are lacking in the literature. PATIENTS AND METHODS: Using the Surveillance, Epidemiology, and End Results (SEER) database, we identified all patients with primary malignant osseous neoplasms of the hand diagnosed between 1983 and 2015. Demographic data were searched for primary osseous neoplasms in the hand and higher incidence of histological subtype. RESULTS: A total of 197 patients were analyzed: 103 patients were diagnosed with histologically low-grade tumor, and 31 were diagnosed with high-grade tumor. Five-year cancer-specific and overall survival rates for the entire cohort were 91.4% and 81.9%, respectively. Histological high tumor grade and regional stage from SEER historic stage data were associated with unfavorable cancer-specific survival. CONCLUSION: Special caution is required if patients have histologically high-grade tumor or tumor extending beyond the periosteum into surrounding joints, as these features worsen cancer-specific mortality.
• The effect of local injection of tranexamic acid into peri-articular tissue versus drain clamping in total knee arthroplasty: a randomized controlled trial.

  Ryosuke Hishimura, Tomohiro Onodera, Yasumitsu Ohkoshi, Kazufumi Okada, Masatake Matsuoka, Shinji Matsubara, Koji Iwasaki, Eiji Kondo, Norimasa Iwasaki

  BMC musculoskeletal disorders 23 1 111 - 111 2022年02月02日 

  BACKGROUND: Tranexamic acid (TXA) is used as a synthetic anti-fibrinolytic agent for total knee arthroplasty (TKA) to reduce postoperative bleeding. Though the effects on bleeding reduction of several methods of administering TXA have been demonstrated, the optimal method remains controversial. Recently, the hemostatic effect of periarticular local injection of TXA during TKA was reported. Although this method can be expected to suppress postoperative bleeding without placing a drain, its hemostatic effect has not yet been assessed in comparison with local injection and other methods of administering TXA. The aim of this randomized, prospective study was to assess the efficacy of local injection of TXA during TKA. METHODS: To confirm the effect of the local injection of TXA, drain clamping was set as the control. The subjects included a prospective series of 109 patients randomly divided into 2 groups: the local injection (group L) and the drain clamping (group D). The main outcome measure was postoperative bleeding. Secondary outcomes included pain, physical measurements, and laboratory findings. RESULTS: The calculated total blood loss (CTBL) in groups L and D was nearly equal and did not show the non-inferiority of group L to group D (883 ± 248 vs. 841 ± 257 ml, P = .564). Drained blood loss was significantly higher in group L than in group D (395 ± 130 vs 276 ± 78.8 ml, P < .0001). There was no significant difference in hidden blood loss between the groups (488 ± 269 vs 565 ± 261 ml, P = .131). The other laboratory findings and physical measurements were identical between the groups. CONCLUSIONS: Although CTBL in group L did not show non-inferiority to group D, the local injection of TXA was considered to be superior for suppressing bleeding considering the risk of the adverse effects of using a drain. TRIAL REGISTRATION: This was a randomized, prospective study registered with UMIN Clinical Trials Registry (Registration number: UMIN000036146, date of disclosure: 10/3/2019).
• 変形性関節症の病態におけるマクロファージの役割

  小野寺 智洋, 江畑 拓, 濱崎 雅成, 照川 アラー, 岩崎 倫政

  リウマチ科 67 2 216 - 220 (有)科学評論社 2022年02月
• Discrepancy in the Distribution Patterns of Subchondral Bone Density Across the Ankle Joint After Medial Opening-Wedge and Lateral Closing-Wedge High Tibial Osteotomy.

  Shinji Matsubara, Tomohiro Onodera, Koji Iwasaki, Ryosuke Hishimura, Masatake Matsuoka, Eiji Kondo, Norimasa Iwasaki

  The American journal of sports medicine 50 2 478 - 485 2022年02月 

  BACKGROUND: High tibial osteotomy (HTO) changes the alignment and dynamics of the ankle joint; however, differences in the stress distribution of the ankle joint after opening-wedge HTO (OWHTO) and closing-wedge HTO (CWHTO) are not understood. It is believed that subchondral bone density of the articular surface reflects the pattern of cumulative stress distribution across the joint surface. PURPOSE: To clarify the effects of OWHTO and CWHTO on the distribution patterns of subchondral bone density across the ankle joint using computed tomography (CT)-osteoabsorptiometry. STUDY DESIGN: Cohort study; Level of evidence, 4. METHODS: Radiographic and CT data of 18 cases who underwent OWHTO (OW group), 12 cases who underwent CWHTO (CW group), and 11 cases with unilateral anterior cruciate ligament injury serving as controls were retrospectively reviewed. The subchondral bone density of the distal tibia was assessed in the 3 groups using CT-osteoabsorptiometry. The distal tibial surface of the ankle joint was divided into 4 parts in the coronal direction, and the percentage of the high-density area (%HDA) to each subregion was compared before and after HTO. RESULTS: Preoperatively, comparing %HDA among the 3 groups, there were no significant differences in any regions. In the OW group, postoperative %HDA in the most medial region was significantly increased compared with preoperative %HDA (49.3% to 53.0%; P = .011), and postoperative %HDA in the most lateral region was significantly decreased (21.4% to 17.2%; P = .003). On the other hand, in the CW group, postoperative %HDA in the most medial region was significantly decreased (55.7% to 35.7%; P = .001), and %HDA in the second lateral region was significantly increased (23.6% to 29.2%; P = .002). CONCLUSION: The ankle distribution pattern of subchondral bone density shifted significantly medially after OWHTO without fibular osteotomy, whereas the distribution pattern shifted laterally after CWHTO with fibular osteotomy. When the OWHTO is performed for patients with medial ankle osteoarthritis, surgeons should pay attention to potential postoperative progression of ankle osteoarthritis due to medial shift of the stress distribution in the ankle joint.
• Establishment of a New Qualitative Evaluation Method for Articular Cartilage by Dynamic T2w MRI Using a Novel Contrast Medium as a Water Tracer.

  Yoshiaki Hosokawa, Tomohiro Onodera, Kentaro Homan, Jun Yamaguchi, Kohsuke Kudo, Hiroyuki Kameda, Hiroyuki Sugimori, Norimasa Iwasaki

  Cartilage 13 3 19476035221111503 - 19476035221111503 2022年07月 

  Objective In the early stages of cartilage damage, diagnostic methods focusing on the mechanism of maintaining the hydrostatic pressure of cartilage are thought to be useful. O-17-labeled water, which is a stable isotope of oxygen, has the advantage of no radiation exposure or allergic reactions and can be detected by magnetic resonance imaging (MRI). This study aimed to evaluate MRI images using O-17-labeled water in a rabbit model. Design Contrast MRI with O-17-labeled water and macroscopic and histological evaluations were performed 4 and 8 weeks after anterior cruciate ligament transection surgery in rabbits. A total of 18 T2-weighted images were acquired, and O-17-labeled water was manually administered on the third scan. The O-17 concentration in each phase was calculated from the signal intensity at the articular cartilage. Macroscopic and histological grades were evaluated and compared with the O-17 concentration. Results An increase in O-17 concentration in the macroscopic and histologically injured areas was observed by MRI. Macroscopic evaluation showed that the O-17 concentration significantly increased in the damaged site group. Histological evaluations also showed that O-17 concentrations significantly increased at 36 minutes 30 seconds after initiating MRI scanning in the Osteoarthritis Research Society International (OARSI) grade 3 (0.493 in grade 0, 0.659 in grade 1, 0.4651 in grade 2, and 0.9964 in grade 3, P < 0.05). Conclusion O-17-labeled water could visualize earlier articular cartilage damage, which is difficult to detect by conventional methods.
• Surgical resection of primary tumor in the extremities improves survival for metastatic soft-tissue sarcoma patients: a population-based study of the SEER database.

  M Matsuoka, T Onodera, I Yokota, K Iwasaki, S Matsubara, R Hishimura, E Kondo, N Iwasaki

  Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 23 12 2474 - 2481 2021年12月 

  PURPOSE: The objectives of this study were to clarify whether resection of primary tumor in the extremities for patients with metastatic soft-tissue sarcoma (STS) improves survival, and to clarify patient groups for whom primary tumor resection should be considered. METHODS/PATIENTS: Using the surveillance, epidemiology, and end results database, we identified 1453 patients with metastatic STS of the extremities at initial presentation between 1983 and 2016. Of these 1453 patients, 898 patients underwent primary tumor resection (Surgery group), and 555 patients did not (No-surgery group). RESULTS: After adjusting for patient background by propensity score matching, a total of 804 patients were included for analysis. Patients in the Surgery group showed improved survival (cancer-specific survival (CSS) hazard ratio (HR) = 0.59, 95% confidence interval (CI) 0.50-0.71 overall survival rate (OS) HR = 0.60, 95% CI 0.51-0.70). In subclass analysis, patients with high-grade STS, undifferentiated pleomorphic sarcoma, leiomyosarcoma, or synovial sarcoma showed improved survival in the Surgery group (high grade-CSS HR = 0.57, 95% CI 0.45-0.72, OS HR = 0.58, 95% CI 0.48-0.71; undifferentiated pleomorphic sarcoma-CSS HR = 0.60, 95% CI 0.42-0.84, OS HR = 0.61, 95% CI 0.46-0.82; leiomyosarcoma-CSS HR = 0.50, 95% CI 0.33-0.75, OS HR = 0.50, 95% CI 0.35-0.72; synovial sarcoma-CSS HR = 0.46, 95% CI 0.31-0.68, OS HR = 0.43, 95% CI 0.30-0.62). CONCLUSIONS: Our results indicated that primary tumor resection in metastatic STS exerts positive impacts on survival. Further clinical research is needed to confirm these results.
• Quantification of Cartilage Surface Degeneration by Curvature Analysis Using 3D Scanning in a Rabbit Model.

  Dawei Liang, Tomohiro Onodera, Masanari Hamasaki, Ryosuke Hishimura, Kentaro Homan, Liang Xu, Yuan Tian, Satoshi Kanai, Norimasa Iwasaki

  Cartilage 13 2_suppl 1734S-1741S  2021年12月 

  OBJECTIVE: Accurate analysis to quantify cartilage morphology is critical for evaluating degenerative conditions in osteoarthritis (OA). Three-dimensional (3D) optical scanning provides 3D data for the entire cartilage surface; however, there is no consensus on how to quantify it. Our purpose was to validate a 3D method for evaluating spatiotemporal alterations in degenerative cartilages in a rabbit OA model by analyzing their curvatures at various stages of progression. DESIGN: Twelve rabbits underwent anterior cruciate ligament transection (ACLT) unilaterally and were divided into 4 groups: 4 weeks control, 4 weeks OA, 8 weeks control, and 8 weeks OA. 3D scanning, India ink staining, and histological assessments were performed in all groups. In 3D curvature visualization, the surfaces of the condyles were divided into 8 areas. The standard deviations (SD) of mean curvatures from all vertices of condylar surfaces and subareas were calculated. RESULTS: Regarding the site of OA change, curvature analysis was consistent with India ink scoring. The SD of mean curvature correlated strongly with the India ink Osteoarthritis Research Society International (OARSI) score. In curvature histograms, the curvature distribution in OA was more scattered than in control. Of the 8 areas, significant OA progression in the posterolateral part of the lateral condyle (L-PL) was observed at 4 weeks. The histology result was consistent with the 3D evaluation in terms of representative section. CONCLUSIONS: This study demonstrated that 3D scanning with curvature analysis can quantify the severity of cartilage degeneration objectively. Furthermore, the L-PL was found to be the initial area where OA degeneration occurred in the rabbit ACLT model.
• No patella resurfacing total knee arthroplasty leads to reduction in the thickness of patellar cartilage to less than half within 5 years: a quantitative longitudinal evaluation using MRI.

  Dai Sato, Masayuki Inoue, Takuro Sasaki, Jun Uchida, Tomohiro Onodera, Eiji Kondo, Norimasa Iwasaki

  Journal of experimental orthopaedics 8 1 107 - 107 2021年11月24日 

  PURPOSE: Patellar resurfacing in total knee arthroplasty (TKA) remains controversial as recent meta-analyses have not shown its clear superiority; however, most authors recommend it because it is associated with less frequent anterior knee pain and need for reoperation. We aimed to clarify the changes in patellar cartilage thickness in no patellar resurfacing TKA using a ceramic femoral component on magnetic resonance imaging (MRI). METHODS: Between 2009 and 2014, 40 consecutive patients (59 knees) were included in this study. All patients underwent TKA using zirconia ceramic femoral implants without patellar resurfacing. Indications for no patellar resurfacing TKA were absence of anterior knee pain, patellar compression pain, and osteoarthritic changes in the patellofemoral joint on plain radiography. The mean postoperative follow-up duration was 81.5 months (range, 25-131 months). Clinical and radiological evaluations were performed preoperatively and 5 years after TKA. Patellar cartilage thickness was evaluated preoperatively and every year for 5 years after TKA using MRI T2-weighted imaging. The patellar cartilage was divided into three regions of interest: medial, central, and lateral. To standardise the variation in patellar thickness among patients, the percent cartilage thickness was calculated. RESULTS: The implant's position was appropriate in all cases. Compared to preoperative scores, 5 years postoperatively, the Japanese Orthopedic Association score and Oxford knee score significantly improved from 52.1 to 84.7; mean tilting angle and congruence angle did not change significantly; mean lateral shift ratio significantly increased from 7.1% to 14.6%; cartilage thickness significantly decreased (P < 0.05); and the percentage cartilage thickness of the central, medial, and lateral cartilage zones gradually thinned to less than half. Four patients underwent conversion to patellar resurfacing due to anterior knee pain, without loosening the femoral and tibial implants. CONCLUSION: The patellar cartilage thickness decreased to less than half its preoperative level within 5 years after no patellar resurfacing TKA; this would led to clinical problems and conversion to patellar resurfacing. LEVEL OF EVIDENCE: Level III.
• 関節リウマチ患者の2010年以降の下肢手術数の動向と併存症発生に関する調査

  糸賀 稜, 清水 智弘, 高橋 大介, 小野寺 智洋, 岩崎 倫政

  日本関節病学会誌 40 3 285 - 285 (一社)日本関節病学会 2021年11月
• Brain Metastasis in Soft Tissue Sarcoma at Initial Presentation.

  Ryo Itoga, Masatake Matsuoka, Tomohiro Onodera, Isao Yokota, Koji Iwasaki, Shinji Matsubara, Ryosuke Hishimura, Yuki Suzuki, Akira Iwata, Eiji Kondo, Norimasa Iwasaki

  Anticancer research 41 11 5611 - 5616 2021年11月 

  BACKGROUND/AIM: Brain metastasis is a rare condition among patients with soft tissue sarcoma (STS), and its precise incidence remains unclear. The aim of this study was to investigate which patients should be screened for brain metastasis. PATIENTS AND METHODS: We identified all patients with STS diagnosed between 2010 and 2015 in the SEER database. Incidence of brain metastasis at initial presentation and higher incidence of brain metastasis by histological subtype were investigated. In addition, risk factors for brain metastasis were examined. RESULTS: A total of 26,676 patients were included for analysis, of whom 162 patients (0.6%) had brain metastasis. Alveolar soft part sarcoma (6.3%), malignant hemangioendothelioma (3.1%) and malignant schwannoma (2.6%) showed higher incidence of brain metastasis. Deep-rooted tumor, trunk tumor, and histological high-grade tumor tended to show higher incidence of brain metastasis. CONCLUSION: Risk factors for brain metastasis were deep location, trunk development and histologically high-grade tumor, or specific histological subtypes.
• Stress on the posteromedial region of the proximal tibia increased over time after anterior cruciate ligament injury.

  Soya Miura, Koji Iwasaki, Eiji Kondo, Kaori Endo, Shinji Matsubara, Masatake Matsuoka, Tomohiro Onodera, Norimasa Iwasaki

  Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA 30 5 1744 - 1751 2021年09月10日 

  PURPOSE: Anterior cruciate ligament (ACL) injury induces anterior and rotatory instability of the knee. However, the effect of this instability on the stress distribution in the knee joint in living participants is not clear. The aim of this study was to compare the distribution pattern of subchondral bone density across the proximal tibia in the knees with and without ACL injury, and to investigate the correlation between the distribution patterns of the subchondral bone density and the duration of ACL-deficiency. METHODS: Radiographic and computed tomography (CT) data pertaining to 20 patients with unilateral ACL injury without combined injury (ACL-deficient group) and 19 nontraumatic subjects (control group) were collected retrospectively. Subchondral bone density of the proximal tibia was assessed using CT-osteoabsorptiometry. Both the medial and lateral compartments of the proximal tibia were divided into three subregions of equal width in the sagittal direction. The percentage of high subchondral bone density areas (HDA%) in each subregion was quantitatively analyzed. RESULTS: HDA% of the posteromedial region was significantly higher in the ACL-deficient group (mean: 21.6%) than in the control group (14.7%) (p = 0.002). In contrast, HDA% of the anteromedial region was significantly lower in the ACL-deficient group (9.4%) than in the control group (15.3%) (p = 0.048). The logarithm of the time elapsed from ACL injury to CT examination showed a significant correlation with HDA% in the posteromedial region (p = 0.032). CONCLUSIONS: Subchondral bone density in the posteromedial region significantly increased after ACL injury and correlated with the duration of ACL-deficiency in semi-log manner in meniscus intact knees. The increase in stress on the posteromedial region after ACL injury, which induces a change in the subchondral bone density, justifies early ACL reconstruction after ACL injury.
• Medial closed-wedge distal femoral osteotomy with local bone grafts for large collapsed steroid-induced osteonecrosis of the lateral femoral condyle: A case report.

  Takuma Kaibara, Eiji Kondo, Masatake Matsuoka, Koji Iwasaki, Tomohiro Onodera, Daisuke Momma, Norimasa Iwasaki

  Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association 2021年08月04日
• 大腿骨顆間外側壁における骨密度分布 前十字靱帯の大腿骨付着部についての考察

  菅原 悠太郎, 岩崎 浩司, 遠藤 香織, 菱村 亮介, 松原 新史, 松岡 正剛, 門間 太輔, 小野寺 智洋, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 95 8 S1715 - S1715 (公社)日本整形外科学会 2021年08月
• 遠隔転移を有する四肢発生軟部肉腫症例において原発巣切除が生存に与える影響

  松岡 正剛, 小野寺 智洋, 横田 勲, 岩崎 浩司, 松原 新史, 菱村 亮介, 近藤 英司, 岩崎 倫政

  東日本整形災害外科学会雑誌 33 3 383 - 383 東日本整形災害外科学会 2021年08月
• 軟部肉腫患者において初診時脳転移発生に寄与するリスク因子の探索 米国SEERデータベースを用いた研究

  糸賀 稜, 松岡 正剛, 小野寺 智洋, 横田 勲, 岩崎 浩司, 松原 新史, 菱村 亮介, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 95 8 S1550 - S1550 (公社)日本整形外科学会 2021年08月
• 手指原発の悪性骨腫瘍の検討

  池 翔太, 松岡 正剛, 小野寺 智洋, 横田 勲, 岩崎 浩司, 菱村 亮介, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 95 8 S1557 - S1557 (公社)日本整形外科学会 2021年08月
• 関節リウマチ患者の下肢手術数の傾向と併存症発生に関する調査

  糸賀 稜, 清水 智弘, 高橋 大介, 小野寺 智洋, 岩崎 倫政

  東日本整形災害外科学会雑誌 33 3 285 - 285 東日本整形災害外科学会 2021年08月
• 炎症性マクロファージ由来細胞外小胞を介した軟骨変性メカニズムの解明

  江畑 拓, Terkawi Alaa, 松前 元, 横田 隼一, Alhasan Hend, 清水 智弘, 高橋 大介, 本谷 和俊, 小野寺 智洋, 角家 健, 岩崎 倫政

  日本整形外科学会雑誌 95 8 S1520 - S1520 (公社)日本整形外科学会 2021年08月
• 炎症性マクロファージ由来細胞外小胞を介した軟骨変性メカニズムの解明

  江畑 拓, Terkawi Alaa, 松前 元, 横田 隼一, Alhasan Hend, 清水 智弘, 高橋 大介, 本谷 和俊, 小野寺 智洋, 角家 健, 岩崎 倫政

  日本整形外科学会雑誌 95 8 S1520 - S1520 (公社)日本整形外科学会 2021年08月
• 遠隔転移を有する四肢発生軟部肉腫症例において原発巣切除が生存に与える影響

  松岡 正剛, 小野寺 智洋, 横田 勲, 岩崎 浩司, 松原 新史, 菱村 亮介, 近藤 英司, 岩崎 倫政

  東日本整形災害外科学会雑誌 33 3 383 - 383 東日本整形災害外科学会 2021年08月
• 軟部肉腫患者において初診時脳転移発生に寄与するリスク因子の探索 米国SEERデータベースを用いた研究

  糸賀 稜, 松岡 正剛, 小野寺 智洋, 横田 勲, 岩崎 浩司, 松原 新史, 菱村 亮介, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 95 8 S1550 - S1550 (公社)日本整形外科学会 2021年08月
• 手指原発の悪性骨腫瘍の検討

  池 翔太, 松岡 正剛, 小野寺 智洋, 横田 勲, 岩崎 浩司, 菱村 亮介, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 95 8 S1557 - S1557 (公社)日本整形外科学会 2021年08月
• 関節リウマチ患者の下肢手術数の傾向と併存症発生に関する調査

  糸賀 稜, 清水 智弘, 高橋 大介, 小野寺 智洋, 岩崎 倫政

  東日本整形災害外科学会雑誌 33 3 285 - 285 東日本整形災害外科学会 2021年08月
• Delayed Onset of Infectious Foreign Body Granuloma for Nonabsorbable Sutures After Open Achilles Tendon Repair: A Case Report.

  Ryo Itoga, Masatake Matsuoka, Tomohiro Onodera, Ai Shimizu, Koji Iwasaki, Shinji Matsubara, Ryosuke Hishimura, Eiji Kondo, Norimasa Iwasaki

  The Journal of foot and ankle surgery : official publication of the American College of Foot and Ankle Surgeons 60 6 1297 - 1300 2021年07月20日 

  Foreign body granulomas from sutures are more common among non-absorbable sutures compared to absorbable sutures and have been reported as a postoperative complication in a variety of medical fields. However, only a few cases of delayed foreign body reaction have been reported and addressed with orthopedics. We present a patient with systemic lupus erythematosus who developed an infectious suture granuloma that extended to the entire Achilles tendon and was induced by nonabsorbable sutures that were used for open Achilles tendon repair 20 years before. This granuloma was resistant to antibiotic treatment and drainage and required surgical intervention. The tumor was marginally resected, and the continuity of the Achilles tendon was preserved. Histopathological examination detected many suture materials surrounded by xanthochromic necrotic lesions and the lesion was diagnosed as a foreign body granuloma. At 1 year after the operation, the patient did not exhibit exacerbation of infection or recurrence of the tumor, and she became able to walk by herself. To the best of our knowledge, this represents the first report of an infectious delayed foreign body granuloma in autoimmune disorder patient after open Achilles tendon repair. Surgical intervention should be considered, as treatment with conservative therapy such as antibiotics may be difficult.
• 初診時遠隔転移を有する四肢発生軟部肉腫症例における原発巣切除の意義 米国SEERデータベースを用いた研究

  松岡 正剛, 小野寺 智洋, 横田 勲, 岩崎 浩司, 松原 新史, 菱村 亮介, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 95 6 S1266 - S1266 (公社)日本整形外科学会 2021年06月
• 軟部肉腫患者において初診時脳転移発生に寄与するリスクファクターの検討 米国SEERデータベースを用いた研究

  糸賀 稜, 松岡 正剛, 小野寺 智洋, 横田 勲, 岩崎 浩司, 松原 新史, 菱村 亮介, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 95 6 S1314 - S1314 (公社)日本整形外科学会 2021年06月
• 遠隔転移を有する脊椎発生悪性骨腫瘍において原発巣切除は生存を改善するか

  松岡 正剛, 小野寺 智洋, 横田 勲, 岩崎 浩司, 菱村 亮介, 岩田 玲, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 95 6 S1348 - S1348 (公社)日本整形外科学会 2021年06月
• 四肢原発骨・軟部肉腫症例における患肢温存術と切断術の比較 米国SEERデータベースを用いた研究

  中條 誠也, 松岡 正剛, 小野寺 智洋, 横田 勲, 岩崎 浩司, 菱村 亮介, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 95 6 S1359 - S1359 (公社)日本整形外科学会 2021年06月
• 手指原発の悪性骨腫瘍の特徴 米国SEERデータベースを用いた研究

  池 翔太, 松岡 正剛, 小野寺 智洋, 横田 勲, 岩崎 浩司, 菱村 亮介, 近藤 英司, 岩崎 倫政

  日本整形外科学会雑誌 95 6 S1396 - S1396 (公社)日本整形外科学会 2021年06月
• Ultrapurified Alginate Gel Containing Bone Marrow Aspirate Concentrate Enhances Cartilage and Bone Regeneration on Osteochondral Defects in a Rabbit Model

  Liang Xu, Atsushi Urita, Tomohiro Onodera, Ryosuke Hishimura, Takayuki Nonoyama, Masanari Hamasaki, Dawei Liang, Kentaro Homan, Jian Ping Gong, Norimasa Iwasaki

  The American Journal of Sports Medicine 036354652110141 - 036354652110141 2021年06月01日 

  Ultrapurified alginate (UPAL) gel implantation has been demonstrated as effective in cartilage repair for osteochondral defects; however, cell transplantation within UPAL gels would be required to treat larger defects. The combination of UPAL gel and bone marrow aspirate concentrate (BMAC) would enhance cartilage repair and subchondral bone repair for large osteochondral defects. Controlled laboratory study. A total of 104 osteochondral defects (1 defect per knee) of 52 rabbits were randomly divided into 4 groups (26 defects per group): defects without any treatment (Defect group), defects treated using UPAL gel alone (UPAL group), defects treated using UPAL gel containing allogenic bone marrow mesenchymal stromal cells (UPAL-MSC group), and defects treated using UPAL gel containing BMAC (UPAL-BMAC group). At 4 and 16 weeks postoperatively, macroscopic and histologic evaluations and measurements of repaired subchondral bone volumes of reparative tissues were performed. Collagen orientation and mechanical properties of the reparative tissue were assessed at 16 weeks. The defects in the UPAL-BMAC group were repaired with hyaline-like cartilage with well-organized collagen structures. The histologic scores at 4 weeks were significantly higher in the UPAL-BMAC group (16.9 ± 2.0) than in the Defect group (4.7 ± 1.9; P < .05), the UPAL group (10.0 ± 3.3; P < .05), and the UPAL-MSC group (12.2 ± 2.9; P < .05). At 16 weeks, the score in the UPAL-BMAC group (24.4 ± 1.7) was significantly higher than those in the Defect group (9.0 ± 3.7; P < .05), the UPAL group (14.2 ± 3.9; P < .05), and the UPAL-MSC group (16.3 ± 3.6; P < .05). At 4 and 16 weeks, the macroscopic evaluations were significantly superior in the UPAL-BMAC group compared with the other groups, and the values of repaired subchondral bone volumes in the UPAL-BMAC group were significantly higher than those in the Defect and UPAL groups. The mechanical properties of the reparative tissues were significantly better in the UPAL-BMAC group than in the other groups. The implantation of UPAL gel containing BMAC-enhanced hyaline-like cartilage repair and subchondral bone repair of osteochondral defects in a rabbit knee model. These data support the potential clinical application of 1-step treatment for large osteochondral defects using biomaterial implantation with cell transplantation.
• Effect of High Tibial Osteotomy on the Distribution of Subchondral Bone Density Across the Proximal Tibial Articular Surface of the Knee With Medial Compartment Osteoarthritis.

  Koji Iwasaki, Eiji Kondo, Shinji Matsubara, Masatake Matsuoka, Kaori Endo, Isao Yokota, Tomohiro Onodera, Norimasa Iwasaki

  The American journal of sports medicine 49 6 1561 - 1569 2021年05月 

  BACKGROUND: The effect of high tibial osteotomy (HTO) on the stress distribution across the knee joint is not completely understood. Subchondral bone density is considered to reflect the pattern of stress distribution across a joint surface. PURPOSE: To assess the distribution of subchondral bone density across the proximal tibia in nonarthritic knees and in the knees of patients with osteoarthritis (OA) before and after HTO. STUDY DESIGN: Cohort study; Level of evidence, 3. METHODS: We retrospectively collected radiological and computed tomography data from 16 patients without OA (control group) and 17 patients with OA. Data from the OA group were collected before and 1.5 years after HTO. Subchondral bone density of the proximal tibia was assessed with computed tomography-osteoabsorptiometry. The locations and percentages represented by high-density areas (HDAs) on the articular surface were quantitatively analyzed. RESULTS: The ratio of the HDA of the medial compartment to the total HDA (medial ratio) was significantly higher in the preoperative OA group (mean, 80.1%) than in the control group (61.3%) (P < .001). After HTO, the medial ratio decreased significantly to 75.1% (P = .035 in comparison with preoperative values) and was significantly correlated with the hip-knee-ankle angle in both groups: control (r = -0.551; P = .033) and OA (r = -0.528; P = .043). The change in medial ratio after HTO was significantly correlated with the change in hip-knee-ankle angle (r = 0.587; P = .035). In the medial compartment, the HDA in the most lateral region of 4 subregions increased after HTO, but that in 3 medial subregions decreased. CONCLUSION: In this exploratory study, HTO shifted the HDA of the medial compartment of the proximal tibial articular surface toward the lateral compartment. In contrast, the HDA of the most lateral region of the medial compartment increased after HTO. This change in subchondral bone density may result from the change in stress distribution.
• Flightless I is a catabolic factor of chondrocytes that promotes hypertrophy and cartilage degeneration in osteoarthritis

  Taku Ebata, Mohamad Alaa Terkawi, Masanari Hamasaki, Gen Matsumae, Tomohiro Onodera, Mahmoud Khamis Aly, Shunichi Yokota, Hend Alhasan, Tomohiro Shimizu, Daisuke Takahashi, Kentaro Homan, Ken Kadoya, Norimasa Iwasaki

  iScience 102643 - 102643 2021年05月
• Changes in the Contact Stress Distribution Pattern of the Patellofemoral Joint After Medial Open-Wedge High Tibial Osteotomy: An Evaluation Using Computed Tomography Osteoabsorptiometry.

  Toshiaki Kameda, Eiji Kondo, Tomohiro Onodera, Koji Iwasaki, Jun Onodera, Kazunori Yasuda, Norimasa Iwasaki

  Orthopaedic journal of sports medicine 9 4 2325967121998050 - 2325967121998050 2021年04月 

  Background: Medial open-wedge high tibial osteotomy (OWHTO) theoretically causes distalization and lateralization of the tibial tuberosity and the patella. Purpose/Hypothesis: The purpose of the study was to identify any changes in the stress distribution of subchondral bone density across the patellofemoral (PF) joint before and after OWHTO through the use of computed tomography (CT) osteoabsorptiometry. We hypothesized that OWHTO would alter the distribution of contact stress in the PF joint. Study Design: Case series; Level of evidence, 4. Methods: A total of 17 patients (17 knees) who underwent OWHTO were enrolled in this study between September 2013 and September 2015. All patients underwent radiologic examination preoperatively and at 1 year postoperatively, and the distribution patterns of subchondral bone density through the articular surface of the femoral trochlea and patella were assessed preoperatively and >1 year postoperatively using CT osteoabsorptiometry. The quantitative analysis of the obtained mapping data focused on location of the high-density area (HDA) through the articular surface of the PF joint. The percentage of HDA at each divided region of the articular surface of the femoral trochlea and the patella was calculated. Results: In the radiologic evaluation, the Blackburne-Peel ratio was significantly reduced (P < .001) after surgery, and the tilting angle of the patella was significantly decreased (P < .001). On CT evaluation, the percentage of HDA in the lateral notch and lateral trochlea of the femur and in the medial portion of the lateral facet of the patella increased significantly after OWHTO surgery (P ≤ .038). Conclusion: OWHTO significantly increased the stress distribution pattern of the lateral trochlea of the femur and the medial portion of the lateral facet of the patella. The procedure significantly lowered the patellar height and significantly decreased the patellar tilting angle after surgery.
• Local Administration of Low-Dose Nerve Growth Factor Antibody Reduced Pain in a Rat Osteoarthritis Model.

  Yuan Tian, Tomohiro Onodera, Mohamad Alaa Terkawi, Koji Iwasaki, Ryosuke Hishimura, Dawei Liang, Takuji Miyazaki, Norimasa Iwasaki

  International journal of molecular sciences 22 5 2021年03月04日 

  Systemic injection of a nerve growth factor (NGF) antibody has been proven to have a significant relevance in relieving osteoarthritis (OA) pain, while its adverse effects remain a safety concern for patients. A local low-dose injection is thought to minimize adverse effects. In this study, OA was induced in an 8-week-old male Sprague-Dawley (SD) rat joint by monoiodoacetate (MIA) injection for 2 weeks, and the effect of weekly injections of low-dose (1, 10, and 100 µg) NGF antibody or saline (control) was evaluated. Behavioral tests were performed, and at the end of week 6, all rats were sacrificed and their knee joints were collected for macroscopic and histological evaluations. Results showed that 100 µg NGF antibody injection relieved pain in OA rats, as evidenced from improved weight-bearing performance but not allodynia. In contrast, no significant differences were observed in macroscopic and histological scores between rats from different groups, demonstrating that intra-articular treatment does not worsen OA progression. These results suggest that local administration yielded a low effective NGF antibody dose that may serve as an alternative approach to systemic injection for the treatment of patients with OA.
• Acellular Cartilage Repair Technique Based on Ultrapurified Alginate Gel Implantation for Advanced Capitellar Osteochondritis Dissecans

  Daisuke Momma, Tomohiro Onodera, Daisuke Kawamura, Atsushi Urita, Yuichiro Matsui, Rikiya Baba, Tadanao Funakoshi, Makoto Kondo, Toshiya Endo, Eiji Kondo, Norimasa Iwasaki

  Orthopaedic Journal of Sports Medicine 9 3 232596712198967 - 232596712198967 2021年03月01日 

  One of the most important limitations of osteochondral autograft transplant is the adverse effect on donor sites in the knee. Ultrapurified alginate (UPAL) gel is a novel biomaterial that enhances hyaline-like cartilage repair for articular defects. To avoid the need for knee cartilage autografting when treating osteochondritis dissecans (OCD) of the capitellum, we developed a surgical procedure involving a bone marrow stimulation technique (BMST) augmented by implantation of UPAL gel. BMST augmented by UPAL gel implantation improves the cartilage repair capacity and provides satisfactory clinical outcomes in OCD of the capitellum. Case series; Level of evidence, 4. A total of 5 athletes with advanced capitellar OCD in the dominant elbow underwent BMST augmented by implantation of UPAL gel. The osteochondral defects were filled with UPAL gel after BMST. At a mean follow-up of 97 weeks, all patients were evaluated clinically and radiographically. At final follow-up, all 5 patients had returned to competitive-level sports, and 4 patients were free from elbow pain. The mean Timmerman-Andrews score significantly improved from 100 to 194 points. Radiographically, all patients exhibited graft incorporation and a normal contour of the subchondral cortex. Magnetic resonance imaging showed that the preoperative heterogeneity of the lesion had disappeared, and the signal intensity had returned to normal. Arthroscopic examinations consistently exhibited improvement in the International Cartilage Regeneration and Joint Preservation Society (ICRS) grade of lesions from 3 or 4 to 1 or 2 in 4 patients at 85 weeks postoperatively. Histologic analysis of biopsy specimens revealed an average total ICRS Visual Assessment Scale II histologic score of 1060. The acellular cartilage repair technique using UPAL gel for advanced capitellar OCD provided satisfactory clinical and radiographic results. The present results suggest that this novel technique is a useful, minimally invasive approach for treating cartilaginous lesions in athletes.
• 関節リウマチ患者の下肢手術数の傾向と併存症発生に関する調査

  糸賀 稜, 清水 智弘, 高橋 大介, 小野寺 智洋, 岩崎 倫政

  日本リウマチ学会北海道・東北支部学術集会抄録集 30回 45 - 45 (一社)日本リウマチ学会-北海道・東北支部 2021年02月
• 大腿骨顆間窩外側壁における骨密度分布 前十字靱帯の大腿骨付着部に関する考察

  菅原 悠太郎, 遠藤 香織, 菱村 亮介, 松原 新史, 松岡 正剛, 小野寺 智洋, 岩崎 倫政, 岩崎 浩司, 門間 大輔, 近藤 英司

  北海道整形災害外科学会雑誌 63 139th suppl 36 - 36 北海道整形災害外科学会 2021年
• Hallux Valgus With Second Brachymetatarsia Discovered Accidentally: A Case Report.

  Shinji Matsubara, Tomohiro Onodera, Koji Iwasaki, Ryosuke Hishimura, Masatake Matsuoka, Eiji Kondo, Norimasa Iwasaki

  The Journal of foot and ankle surgery : official publication of the American College of Foot and Ankle Surgeons 60 5 1060 - 1062 2021年 

  Hallux valgus deformity is one of the most common foot and ankle diseases, while brachymetatarsia is a rare foot anomaly with pathological shortening of a metatarsal bone. We present a case of hallux valgus deformity possibly due to second brachymetatarsia. As the hallux valgus was associated with dorsal dislocation of the second toe that made it difficult to evaluate the length of the second toe, the patient was unaware of the second metatarsal shortening until the lengths of the toes compared by manual reposition of the second MTP joint. In this case, proximal osteotomy of the first metatarsal on the hallux valgus and single-stage bone lengthening of the second metatarsal with iliac bone grafting on the second brachymetatarsia were performed. One year after the operation, the callosity of the third toe resolved and the clinical scores were improved. In the case of a hallux valgus deformity with second dorsal dislocation of the toes, surgeons should consider that there are rare cases with second metatarsal shortening. When hallux valgus associated with second brachymetatarsia is diagnosed, second metatarsal lengthening should be considered in addition to hallux valgus surgery.
• Impact of Smoking History on Pulmonary Metastasis-free Survival in Patients With Soft-tissue Sarcoma.

  Masatake Matsuoka, Masanori Okamoto, Tamotsu Soma, Isao Yokota, Ryuta Arai, Tomohiro Onodera, Eiji Kondo, Norimasa Iwasaki, Hiroaki Hiraga

  Cancer diagnosis & prognosis 1 2 89 - 94 2021年 

  BACKGROUND/AIM: Although smoking history is predictive of poor pulmonary metastasis-free survival (PMFS) in patients with epithelial tumors, the impact of smoking history on PMFS in those with soft-tissue sarcoma (STS) is not known. PATIENTS AND METHODS: Patients undergoing treatment for STS at our institutes between 2008 and 2017 were enrolled. Patients were excluded if they had metastatic lesion, or had a histopathological classification demonstrating small round-cell sarcoma. The impact of smoking history on PMFS and overall survival was examined with multivariate analysis using a Cox proportional hazards model. RESULTS: A total of 250 patients were retrospectively reviewed. Patients with smoking history had worse PMFS on multivariate analysis (hazard ratio=2.00, 95% confidence interval=1.12-3.60). On the other hand, smoking history did not significantly affect overall survival (hazard ratio=1.26, 95% confidence interval=0.61-2.58). CONCLUSION: Patients with STS need to be followed-up by frequent clinical assessments if they have a smoking history.
• 非円形軟部肉腫症例において喫煙歴は肺転移発生の危険因子である

  松岡 正剛, 相馬 有, 新井 隆太, 平賀 博明, 小野寺 智洋, 岩崎 倫政, 横田 勲, 近藤 英司

  北海道整形災害外科学会雑誌 63 139th suppl 19 - 19 北海道整形災害外科学会 2021年
• アキレス腱縫合術20年後に発症した腱縫合糸に対する感染性異物性肉芽腫の1例

  糸賀 稜, 松岡 正剛, 小野寺 智洋, 松原 新史, 菱村 亮介, 岩崎 倫政, 清水 亜衣, 岩崎 浩司, 近藤 英司

  北海道整形災害外科学会雑誌 63 139th suppl 79 - 79 北海道整形災害外科学会 2021年
• Angiosarcoma after revision total knee arthroplasty.

  Ryuichi Fukuda, Masatake Matsuoka, Tomohiro Onodera, Koji Iwasaki, Daisuke Tanaka, Hiroaki Hiraga, Hiromi Kanno-Okada, Yoshihiro Matsuno, Eiji Kondo, Norimasa Iwasaki

  The Knee 28 151 - 158 2021年01月 

  BACKGROUND: Hemarthrosis after total knee arthroplasty (TKA) is a relatively rare complication. Although most cases are effectively treated with conservative therapy, some cases require angiographic embolization or surgical intervention. Angiosarcoma is a rare malignant tumor derived from the vascular endothelium with neovascular hyperplasia and mainly arises in the skin and superficial soft tissue, and less frequently in deep soft tissue and bone. Although malignant neoplasms such as angiosarcoma in the vicinity of orthopedic implants were reported, the causal relationship between development of the malignant tumor and the orthopedic implant is widely debated in the literature. CASE PRESENTATION: We report the case of a 68-year-old female with angiosarcoma that developed in the knee joint 2 years after revision TKA. The patient exhibited severe persistent bleeding, which reached 1000-1400 ml per day for 4 months. Histological analysis of the synovial tissue in the knee joint showed large cells with nuclear atypia. Immunohistochemical staining showed cells that were positive for CD31, CD34, and D2-40, and she was diagnosed with angiosarcoma. The patient underwent an amputation at the level of the thigh, and her general condition immediately improved after the operation. The patient did not exhibit bleeding from the site of amputation, and no local recurrence or distant metastases were detected 1 year after the amputation. CONCLUSIONS: To the best of our knowledge, this represents the first report of angiosarcoma 2 years after revision TKA. Further careful follow up is needed, given the high-grade malignancy.
• 関節リウマチ患者の下肢手術件数の傾向と併存症発生に関する調査

  糸賀 稜, 清水 智弘, 高橋 大介, 小野寺 智洋, 岩崎 倫政

  北海道整形災害外科学会雑誌 63 139th suppl 44 - 44 北海道整形災害外科学会 2021年
• 摩耗軟骨片活性化マクロファージにより軟骨細胞に発現上昇するFlightless Iの機能解析

  江畑 拓, テルカウイ・アラー, 松前 元, 横田 隼一, アルハサン・ヘンド, 濱崎 雅成, 清水 智弘, 高橋 大介, 小野寺 智洋, 岩崎 倫政

  北海道整形災害外科学会雑誌 63 139th suppl 63 - 63 北海道整形災害外科学会 2021年
• 変形性膝関節症症例におけるウェアラブルセンサーを用いた歩行評価時の最適なセンサー貼付位置の検討

  佐橋 健人, 千葉 健, 岩崎 浩司, 小野寺 智洋, 岩崎 倫政, 近藤 英司, 遠山 晴一

  北海道整形災害外科学会雑誌 63 139th suppl 35 - 35 北海道整形災害外科学会 2021年
• Alterations of Glycosphingolipid Glycans and Chondrogenic Markers during Differentiation of Human Induced Pluripotent Stem Cells into Chondrocytes.

  Liang Xu, Hisatoshi Hanamatsu, Kentaro Homan, Tomohiro Onodera, Takuji Miyazaki, Jun-Ichi Furukawa, Kazutoshi Hontani, Yuan Tian, Rikiya Baba, Norimasa Iwasaki

  Biomolecules 10 12 2020年12月01日 

  Due to the limited intrinsic healing potential of cartilage, injury to this tissue may lead to osteoarthritis. Human induced pluripotent stem cells (iPSCs), which can be differentiated into chondrocytes, are a promising source of cells for cartilage regenerative therapy. Currently, however, the methods for evaluating chondrogenic differentiation of iPSCs are very limited; the main techniques are based on the detection of chondrogenic genes and histological analysis of the extracellular matrix. The cell surface is coated with glycocalyx, a layer of glycoconjugates including glycosphingolipids (GSLs) and glycoproteins. The glycans in glycoconjugates play important roles in biological events, and their expression and structure vary widely depending on cell types and conditions. In this study, we performed a quantitative GSL-glycan analysis of human iPSCs, iPSC-derived mesenchymal stem cell like cells (iPS-MSC like cells), iPS-MSC-derived chondrocytes (iPS-MSC-CDs), bone marrow-derived mesenchymal stem cells (BMSCs), and BMSC-derived chondrocytes (BMSC-CDs) using glycoblotting technology. We found that GSL-glycan profiles differed among cell types, and that the GSL-glycome underwent a characteristic alteration during the process of chondrogenic differentiation. Furthermore, we analyzed the GSL-glycome of normal human cartilage and found that it was quite similar to that of iPS-MSC-CDs. This is the first study to evaluate GSL-glycan structures on human iPS-derived cartilaginous particles under micromass culture conditions and those of normal human cartilage. Our results indicate that GSL-glycome analysis is useful for evaluating target cell differentiation and can thus support safe regenerative medicine.
• 摩耗軟骨片による滑膜炎が誘導する軟骨細胞新規分泌因子「Flightless I」の機能解析

  江畑 拓, Terkawi Alaa, 小野寺 智洋, 木田 博朗, 松前 元, 田 園, Alhasan Hend, 濱崎 雅成, 清水 智弘, 高橋 大介, 岩崎 倫政

  日本整形外科学会雑誌 94 8 S1690 - S1690 (公社)日本整形外科学会 2020年09月
• ヒトiPS細胞の軟骨分化誘導過程におけるスフィンゴ糖脂質糖鎖の網羅的発現解析

  徐 亮, 花松 久寿, 宝満 健太郎, 小野寺 智洋, 古川 潤一, 本谷 和俊, 宮崎 拓自, 田 園, 岩崎 倫政

  日本整形外科学会雑誌 94 8 S1815 - S1815 (公社)日本整形外科学会 2020年09月
• 細胞特異的糖鎖解析による残存未分化iPS細胞の定量的評価法の確立

  宮崎 拓自, 花松 久寿, 小野寺 智洋, 徐 亮, 古川 潤一, 宝満 健太郎, 川嵜 敏祐, 岩崎 倫政

  日本整形外科学会雑誌 94 8 S1874 - S1874 (公社)日本整形外科学会 2020年09月
• 関節軟骨におけるフコシル化糖鎖の欠損は可逆的修復を阻害し軟骨変性を進行させる

  宝満 健太郎, 小野寺 智洋, 古川 潤一, 花松 久寿, 濱崎 雅成, 徐 亮, 宮崎 拓自, 細川 吉暁, 岩崎 倫政

  日本整形外科学会雑誌 94 8 S1970 - S1970 (公社)日本整形外科学会 2020年09月
• ヒトiPS細胞の軟骨分化誘導過程におけるスフィンゴ糖脂質糖鎖の網羅的発現解析

  徐 亮, 花松 久寿, 宝満 健太郎, 小野寺 智洋, 古川 潤一, 本谷 和俊, 宮崎 拓自, 田 園, 岩崎 倫政

  日本整形外科学会雑誌 94 8 S1815 - S1815 (公社)日本整形外科学会 2020年09月
• 細胞特異的糖鎖解析による残存未分化iPS細胞の定量的評価法の確立

  宮崎 拓自, 花松 久寿, 小野寺 智洋, 徐 亮, 古川 潤一, 宝満 健太郎, 川嵜 敏祐, 岩崎 倫政

  日本整形外科学会雑誌 94 8 S1874 - S1874 (公社)日本整形外科学会 2020年09月
• 関節軟骨におけるフコシル化糖鎖の欠損は可逆的修復を阻害し軟骨変性を進行させる

  宝満 健太郎, 小野寺 智洋, 古川 潤一, 花松 久寿, 濱崎 雅成, 徐 亮, 宮崎 拓自, 細川 吉暁, 岩崎 倫政

  日本整形外科学会雑誌 94 8 S1970 - S1970 (公社)日本整形外科学会 2020年09月
• Large osteochondral defect in the lateral femoral condyle reconstructed by Atelocollagen-associated autologous chondrocyte implantation combined with anterior cruciate ligament reconstruction.

  Takuma Kaibara, Eiji Kondo, Masatake Matsuoka, Koji Iwasaki, Tomohiro Onodera, Daisuke Momma, Naoki Seito, Susumu Mikami, Norimasa Iwasaki

  BMC musculoskeletal disorders 21 1 494 - 494 2020年07月27日 

  BACKGROUND: Articular surface damage commonly associated with rupture of the anterior cruciate ligament (ACL). Large osteochondral defect, which consists of a severe depression fracture and a large cartilage defect, need to be treated due to deformation of the articular surface as it can impact the clinical outcome of ACL reconstruction. Although autologous chondrocyte implantation is one of the useful options in such cases, it can be questioned whether the reconstruction of the ACL and osteochondral defect should be performed in one procedure alone. CASE PRESENTATION: We report a case of a 38-year-old male with a deep depression fracture extending to the edge of the lateral femoral condyle associated with ACL injury after twisting his right knee while skiing. The patient was successfully treated with tissue-engineered cartilage transplantation covered by the periosteum with an iliac bone graft combined with anatomic double-bundle ACL reconstruction. Histopathological examination of the transplanted cartilage taken at second-look arthroscopy showed a cartilage-like tissue in the middle to deep zone in which the extracellular matrix was largely stained with Safranin O. The patient was able to return to his previous level of skiing activity without any experience of knee pain. Magnetic resonance imaging at 4 years after surgery showed that the graft integrated to the border zone and subchondral bone. The operated knee showed negative Lachman test and had a full range of motion. CONCLUSIONS: To our knowledge, this is the first report of anatomic double-bundle ACL reconstruction with tissue-engineered cartilage transplantation and an iliac bone graft to restore the lateral edge of the femoral condyle.
• Role of Chondrocytes in the Development of Rheumatoid Arthritis Via Transmembrane Protein 147-Mediated NF-κB Activation.

  Mitsutoshi Ota, Yuki Tanaka, Ikuma Nakagawa, Jing-Jing Jiang, Yasunobu Arima, Daisuke Kamimura, Tomohiro Onodera, Norimasa Iwasaki, Masaaki Murakami

  Arthritis & rheumatology (Hoboken, N.J.) 72 6 931 - 942 2020年06月 

  OBJECTIVE: We have previously reported that the coactivation of NF-κB and STAT3 in nonimmune cells, including synovial fibroblasts, enhances the expression of NF-κB target genes and plays a role in chronic inflammation and rheumatoid arthritis (RA). This study was undertaken to examine the role of NF-κB activation in chondrocytes and better understand the pathogenesis of RA. Furthermore, transmembrane protein 147 (TMEM147) was investigated as a representative NF-κB activator in chondrocytes. METHODS: Clinical samples from RA patients were analyzed by immunohistochemistry. Specimens obtained from patients with polydactyly were used as control samples. The functional contribution of chondrocytes and TMEM147 to arthritis was examined in several murine models of RA. In vitro experiments (quantitative polymerase chain reaction, RNA interference, immunoprecipitation, and confocal microscopy) were performed to investigate the mechanism of action of TMEM147 in chondrocytes. RESULTS: Samples obtained from RA patients and mouse models of RA showed coactivation of NF-κB and STAT3 in chondrocytes (P < 0.001). This coactivation induced a synergistic expression of NF-κB targets in vitro (P < 0.01). Chondrocyte-specific deletion of STAT3 significantly suppressed the development of cytokine-induced RA (P < 0.01). TMEM147 was highly expressed in chondrocytes from RA patient samples and the mouse models of RA. Gene silencing of TMEM147 or anti-TMEM147 antibody treatment inhibited the cytokine-mediated activation of NF-κB in vitro (P < 0.01) and suppressed cytokine-induced RA in vivo (P < 0.01). Mechanistically, TMEM147 molecules acted as scaffold proteins for the NF-κB complex, which included breakpoint cluster region and casein kinase 2, and enhanced NF-κB activity. CONCLUSION: These results suggest that chondrocytes play a role in the development of RA via TMEM147-mediated NF-κB activation and indicate a novel therapeutic strategy for RA.
• Therapeutic effects and adaptive limits of an acellular technique by ultrapurified alginate (UPAL) gel implantation in canine osteochondral defect models.

  Tomohiro Onodera, Rikiya Baba, Yasuhiko Kasahara, Toshihiko Tsuda, Norimasa Iwasaki

  Regenerative therapy 14 154 - 159 2020年06月 

  Background: The aim of this study was to clarify the objective therapeutic effects of an acellular technique by ultrapurified alginate (UPAL) gel implantation in canine osteochondral defect models. Methods: Two osteochondral defects (diameters: 3.0 and 5.0 mm) were created on each patellar groove in both knees of 10 dogs. Defects were divided into four groups (n = 10 each): Group 1, untreated 3.0-mm defect; Group 2, 3.0-mm defect with UPAL gel; Group 3, untreated 5.0-mm defect; and Group 4, 5.0-mm defect with UPAL gel. All surgical procedures were performed by individuals unfamiliar with the technique at an independent institution. Articular surfaces were evaluated grossly and histologically at 27 weeks after operation. Results: UPAL gel-treated osteochondral defects showed significantly improved gross appearance in Group 4 and histological appearance in Groups 2 and 4. Reparative tissues in the 3.0-mm defect with UPAL gel were replaced by hyaline-like cartilage tissue. The 5.0-mm defects with UPAL gel were mostly covered with fibrocartilaginous tissue, whereas UPAL gel-untreated defects mostly remained uncovered by any tissue. Conclusions: Although an acellular technique using UPAL gel implantation significantly enhanced osteochondral repair in canines, reparative tissues of the large defect with alginate gel comprised of fibrocartilaginous tissue. This surgical technique is effective, especially for small cartilage injuries. Further improvements are required before clinical application in cases of severe osteochondral defects in humans.
• Clinical Outcomes and Complications During and After Medial Open-Wedge High Tibial Osteotomy Using a Locking Plate: A 3- to 7-Year Follow-up Study.

  Koji Yabuuchi, Eiji Kondo, Jun Onodera, Tomohiro Onodera, Tomonori Yagi, Norimasa Iwasaki, Kazunori Yasuda

  Orthopaedic journal of sports medicine 8 6 2325967120922535 - 2325967120922535 2020年06月 

  Background: Outcomes and complications at mid- or long-term follow-up after medial open-wedge high tibial osteotomy (MOWHTO) with the TomoFix locking plate have not been fully evaluated. Purpose: To evaluate the complications and midterm clinical outcomes after MOWHTO using a TomoFix. Study Design: Case series; Level of evidence, 4. Methods: Enrolled in this study were 80 patients (85 knees) who underwent MOWHTO with the TomoFix locking plate between 2009 and 2013. There were 66 women and 14 men, with a mean age of 61.5 years at the time of surgery. The diagnosis was medial osteoarthritis in 76 knees and spontaneous osteonecrosis of the knee in 9 knees. Metal removal and second-look arthroscopy were performed in all cases. Clinical and radiological examinations were performed at final follow-up after surgery (mean, 4.5 years). Results: The mean Japanese Orthopaedic Association score and Knee injury and Osteoarthritis Outcome Score improved significantly from pre- to postoperatively (P < .0001). The weightbearing line percentage shifted to pass through a point 67.7% lateral from the medial edge of the tibial plateau. The Caton-Deschamps index changed significantly from 0.88 to 0.66 at final follow-up (P < .0001). The mean posterior tibial slope changed significantly from 8.9° to 11.9° at final follow-up (P < .0001). Limb length was significantly increased after MOWHTO (10.3 mm; P < .0001). During plate removal, 14 locking screws were found to be broken in 9 knees (10.6%). The articular cartilage grade of the patellofemoral joint was significantly higher in the second arthroscopy than in the first arthroscopy (P < .0001). The cumulative rate of all complications was 41.2%, with major complications (ie, those requiring additional or extended treatment) in 24.7%. Conclusion: Postoperative outcome scores indicated significant improvement after MOWHTO, although the cumulative rate of all complications was 41.2% and the rate of major complications was 24.7%. These results indicate that MOWHTO with the TomoFix is a technically demanding procedure. Careful preoperative planning and meticulous surgical technique are needed to decrease the incidence of complications associated with MOWHTO.
• Transcriptional profiling of murine macrophages stimulated with cartilage fragments revealed a strategy for treatment of progressive osteoarthritis.

  Masanari Hamasaki, Mohamad Alaa Terkawi, Tomohiro Onodera, Yuan Tian, Taku Ebata, Gen Matsumae, Hend Alhasan, Daisuke Takahashi, Norimasa Iwasaki

  Scientific reports 10 1 7558 - 7558 2020年05月05日 [査読有り][通常論文]
   

  Accumulating evidence suggests that synovitis is associated with osteoarthritic process. Macrophages play principal role in development of synovitis. Our earlier study suggests that interaction between cartilage fragments and macrophages exacerbates osteoarthritic process. However, molecular mechanisms by which cartilage fragments trigger cellular responses remain to be investigated. Therefore, the current study aims at analyzing molecular response of macrophages to cartilage fragments. To this end, we analyzed the transcriptional profiling of murine macrophages exposed to cartilage fragments by RNA sequencing. A total 153 genes were differentially upregulated, and 105 genes were down-regulated in response to cartilage fragments. Bioinformatic analysis revealed that the most significantly enriched terms of the upregulated genes included scavenger receptor activity, integrin binding activity, TNF signaling, and toll-like receptor signaling. To further confirm our results, immunohistochemical staining was performed to detected regulated molecules in synovial tissues of OA patients. In consistence with RNA-seq results, MARCO, TLR2 and ITGα5 were mainly detected in the intima lining layer of synovial tissues. Moreover, blockade of TLR2 or ITGα5 but not Marco using specific antibody significantly reduced production of TNF-α in stimulated macrophages by cartilage fragments. Our data suggested that blocking TLR2 or ITGα5 might be promising therapeutic strategy for treating progressive osteoarthritis.
• Autophagy promotes citrullination of VIM (vimentin) and its interaction with major histocompatibility complex class II in synovial fibroblasts.

  Eri Sugawara, Masaru Kato, Yuki Kudo, Wenshi Lee, Ryo Hisada, Yuichiro Fujieda, Kenji Oku, Toshiyuki Bohgaki, Olga Amengual, Shinsuke Yasuda, Tomohiro Onodera, Shigetsugu Hatakeyama, Tatsuya Atsumi

  Autophagy 16 5 946 - 955 2020年05月 

  We aimed to investigate the involvement of macroautophagy/autophagy in autoimmunity in rheumatoid arthritis (RA) through citrullination of VIM (vimentin) and its interaction with MHC class II in synovial fibroblasts (SFs). The cell surface expression of MHC class II and B7 costimulatory molecules on SFs was analyzed by flow cytometry after treatment with IFNG/IFN-γ (interferon gamma). Intracellular citrullinated autoantigens in SFs were analyzed by immunoblotting using serum from anti-citrullinated peptide antibodies (ACPA)-positive patient as a primary antibody. SFs were incubated in serum-free medium or treated with proteasome inhibitor MG132 to induce autophagy. An autophagy inhibitor 3-methyladenin (3-MA) was used. Intracellular citrullinated VIM (cVIM) was evaluated by immunoblotting and immunocytochemistry. The interaction between MHC class II and cVIM was evaluated with co-immunoprecipitation and proximity ligation assay (PLA). We demonstrated that MHC class II, CD274/B7-H1 and PDCD1LG2/B7-DC were expressed on SFs following treatment with IFNG whereas CD276/B7-H3 was detected on SFs regardless of the presence of IFNG. ACPA-positive sera recognized a 54 kDa protein in SFs. By immunoprecipitation, the 54 kDa protein recognized by RA sera was revealed to be cVIM. Following induction of autophagy, intracellular cVIM was increased in SFs but the effect was canceled by 3-MA. The interaction between MHC class II and cVIM was demonstrated by co-immunoprecipitation. Furthermore, PLA revealed the significant increase of MHC class II-cVIM interaction following induction of autophagy. Our findings suggest that SFs may contribute to the autoimmunity in RA through citrullination of VIM and its interaction with MHC class II promoted by autophagy.Abbreviations: 3-MA: 3-methyladenine; ACPA: anti-citrullinated peptide antibodies; anti-CCP: anti-cyclic citrullinated peptide antibody; cVIM: citrullinated VIM; BECN1: beclin1; DAPI: 4',6-diamidino-2-phenylindole; FBS: fetal bovine serum; HLA: human leukocyte antigen; IFNG/IFN-γ: interferon gamma; IL6: interleukin 6; IP: immunoprecipitation; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MFI: mean fluorescence index; MHC: major histocompatibility complex; OA: osteoarthritis; PADI: peptidyl arginine deiminase; PepA: pepstatin A; PBS: phosphate-buffered saline; PtdIns3K: phosphatidylinositol 3-kinase; RA: rheumatoid arthritis; SFs: synovial fibroblasts; siRNA: small interfering RNA; VIM: vimentin.
• Which Contributes to Meniscal Repair, the Synovium or the Meniscus? An In Vivo Rabbit Model Study With the Freeze-Thaw Method.

  WooYoung Kim, Tomohiro Onodera, Eiji Kondo, Mohamad Alaa Terkawi, Kentaro Homan, Ryosuke Hishimura, Norimasa Iwasaki

  The American journal of sports medicine 48 6 1406 - 1415 2020年05月 [査読有り][通常論文]
   

  BACKGROUND: During meniscal tissue repair, the origin of the reparative cells of damaged meniscal tissue remains unclear. HYPOTHESIS: Comparison of the influence between meniscal and synovial tissues on meniscal repair by the in vivo freeze-thaw method would clarify the origin of meniscal reparative cells. STUDY DESIGN: Controlled laboratory study. METHODS: A total of 48 mature Japanese white rabbits were divided into 4 groups according to the tissue (meniscal or synovial) that received freeze-thaw treatment. The meniscus of each group had a 2 mm-diameter cylindrical defect filled with alginate gel. Macroscopic and histologic evaluations of the reparative tissues were performed at 1, 3, and 6 weeks postoperatively. Additional postoperative measurements included cell density, which was the number of meniscal cells in the cut area per cut area (mm2) of meniscus; cell density ratio, which was the cell density of the sample from each group per the average cell density of the intact meniscus; and cell death rate, which was the number of cells stained by propidium iodide per the number of cells stained by Hoechst 33342 of the meniscal tissue adjacent to the defect. RESULTS: The macroscopic and histologic evaluations of the non-synovium freeze-thaw groups were significantly superior to those of the synovium freeze-thaw groups at 3 and 6 weeks postoperatively. Additionally, the meniscal cell density ratio and cell death rate in the freeze-thaw groups were significantly lower than those in the non-meniscal freeze-thaw groups at 3 and 6 weeks postoperatively. CONCLUSION: The freeze-thawed meniscus recovered few cells in its tissue even after 6 weeks. However, the defect was filled with fibrochondrocytes and proteoglycan when the synovium was intact. On the basis of these results, it is concluded that synovial cells are the primary contributors to meniscal injury repair. CLINICAL RELEVANCE: In meniscal tissue engineering, there is no consensus on the best cell source for meniscal repair. Based on this study, increasing the synovial activity and contribution should be the main objective of meniscal tissue engineering. This study can establish the foundation for future meniscal tissue engineering.
• Rosai-Dorfman disease: earlier spontaneous regression of skin lesions relative to nasal, pharyngeal, and bone lesions.

  Kazumasa Sato, Hideyuki Ujiie, Shinichi Nakazato, Mika Watanabe, Erika Watanabe, Teruki Yanagi, Yuji Nakamaru, Dai Takagi, Ryuta Arai, Tomohiro Onodera, Takeshi Kondo, Takanori Teshima, Hiroshi Shimizu

  European journal of dermatology : EJD 30 2 182 - 183 2020年04月01日
• 軟部肉腫において喫煙歴は肺転移発生の危険因子である

  松岡 正剛, 相馬 有, 新井 隆太, 横田 勲, 小野寺 智洋, 近藤 英司, 岩崎 倫政, 平賀 博明

  日本整形外科学会雑誌 94 3 S576 - S576 (公社)日本整形外科学会 2020年03月
• MicroRNA-9 ameliorates destructive arthritis through down-regulation of NF-κB1-RANKL pathway in fibroblast-like synoviocytes.

  Wen Shi Lee, Shinsuke Yasuda, Michihiro Kono, Yuki Kudo, Sanae Shimamura, Michihito Kono, Yuichiro Fujieda, Masaru Kato, Kenji Oku, Tomohiro Shimizu, Tomohiro Onodera, Norimasa Iwasaki, Tatsuya Atsumi

  Clinical immunology (Orlando, Fla.) 212 108348 - 108348 2020年03月 [査読有り][通常論文]
   

  We investigated the effect of miR-9 on fibroblast-like synoviocytes (FLS) from RA patients and animal arthritis model. The binding of miR-9 to NF-κB1 3'UTR was analyzed by luciferase reporter assay and immunoprecipitation. ChIP assay and luciferase promoter assay were performed to identify the binding of NF-κB1 to RANKL promoter and its activity. FLS were treated with miR-9/anti-miR-9 to evaluate cell proliferation and the expression of RANKL. Therapeutic effect of intra-articular miR-9 was evaluated in type-II collagen-induced arthritis in rats. miR-9 bound to the 3'-UTR of NF-κB1 and downregulated NF-κB1. NF-κB1 bound to RANKL promoter and increased the promoter activity of RANKL. RANKL was downregulated by miR-9. Proliferation of FLS was increased by miR-9 inhibitor. miR-9 dampened experimental arthritis by lowering inflammatory state, reducing RANKL and osteoclasts formation. Our findings revealed miR-9-NF-κB1-RANKL pathway in RA-FLS, further, miR-9 ameliorated inflammatory arthritis in vivo which propose therapeutic implications of miR- 9 in RA.
• 好中球由来細胞外小胞が軟骨細胞に与える影響

  江畑 拓, テルカウイ・アラー, 松前 元, 木田 博朗, 田 園, ヘンド・アルハサン, 高橋 大介, 小野寺 智洋, 岩崎 倫政

  北海道整形災害外科学会雑誌 62 138th suppl 5 - 5 北海道整形災害外科学会 2020年
• 線状強皮症による足関節拘縮に対し全身療法と外科的加療の併用により改善が得られた1例

  山口 哲也, 松岡 正剛, 小野寺 智洋, 松原 新史, 岩崎 倫政, 西江 渉, 清水 亜衣, 後藤 佳子, 近藤 英司, 岩崎 浩司

  北海道整形災害外科学会雑誌 62 138th suppl 41 - 41 北海道整形災害外科学会 2020年
• Blockade of XCL1/Lymphotactin Ameliorates Severity of Periprosthetic Osteolysis Triggered by Polyethylene-Particles.

  Yuan Tian, Mohamad Alaa Terkawi, Tomohiro Onodera, Hend Alhasan, Gen Matsumae, Daisuke Takahashi, Masanari Hamasaki, Taku Ebata, Mahmoud Khamis Aly, Hiroaki Kida, Tomohiro Shimizu, Keita Uetsuki, Ken Kadoya, Norimasa Iwasaki

  Frontiers in immunology 11 1720 - 1720 2020年 

  Periprosthetic osteolysis induced by orthopedic implant-wear particles continues to be the leading cause of arthroplasty failure in majority of patients. Release of the wear debris results in a chronic local inflammatory response typified by the recruitment of immune cells, including macrophages. The cellular mediators derived from activated macrophages favor the osteoclast-bone resorbing activity resulting in bone loss at the site of implant and loosening of the prosthetic components. Emerging evidence suggests that chemokines and their receptors are involved in the progression of periprosthetic osteolysis associated with aseptic implant loosening. In the current study, we investigated the potential role of chemokine C-motif-ligand-1 (XCL1) in the pathogenesis of inflammatory osteolysis induced by wear particles. Expressions of XCL1 and its receptor XCR1 were evident in synovial fluids and tissues surrounding hip-implants of patients undergoing revision total hip arthroplasty. Furthermore, murine calvarial osteolysis model induced by ultra-high molecular weight polyethylene (UHMWPE) particles was used to study the role of XCL1 in the development of inflammatory osteolysis. Mice received single injection of recombinant XCL1 onto the calvariae after implantation of particles exhibited significantly greater osteolytic lesions than the control mice. In contrast, blockade of XCL1 by neutralizing antibody significantly reduced bone erosion and the number of bone-resorbing mature osteoclasts induced by UHMWPE particles. In consistence with the results, transplantation of XCL1-soaked sponge onto calvariae caused osteolytic lesions coincident with excessive infiltration of inflammatory cells and osteoclasts. These results suggested that XCL1 might be involved in the development of periprosthetic osteolysis through promoting infiltration of inflammatory cells and bone resorbing-osteoclasts. Our further results demonstrated that supplementing recombinant XCL1 to cultured human monocytes stimulated with the receptor activator of nuclear factor kappa-B ligand (RANKL) promoted osteoclastogenesis and the osteoclast-bone resorbing activity. Moreover, recombinant XCL1 promoted the expression of inflammatory and osteoclastogenic factors, including IL-6, IL-8, and RANKL in human differentiated osteoblasts. Together, these results suggested the potential role of XCL1 in the pathogenesis of periprosthetic osteolysis and aseptic loosening. Our data broaden knowledge of the pathogenesis of aseptic prosthesis loosening and highlight a novel molecular target for therapeutic intervention.
• Conservatively Treated Femoral Intertrochanteric Fracture With Early Asymptomatic Novel Coronavirus Disease 2019 (COVID-19): A Case Report.

  Yuki Suzuki, Toshihiko Kasashima, Kazutoshi Hontani, Yasuhiro Yamamoto, Kanako Ito, Liang Xu, Masatake Matsuoka, Koji Iwasaki, Tomohiro Onodera, Eiji Kondo, Norimasa Iwasaki

  Geriatric orthopaedic surgery & rehabilitation 11 2151459320969380 - 2151459320969380 2020年 

  Introduction: The ongoing outbreak of novel coronavirus disease 2019 (COVID-19) is a worldwide problem. Although diagnosing COVID-19 in fracture patients is important for selecting treatment, diagnosing early asymptomatic COVID-19 is difficult. We describe herein a rare case of femoral intertrochanteric fracture concomitant with early asymptomatic novel COVID-19. Case presentation: An 87-year-old Japanese woman was transferred to our emergency room with a right hip pain after she fell. She had no fever, fatigue, or respiratory symptoms on admission and within the 14 days before presenting to our hospital, and no specific shadow was detected in chest X-ray. However, chest computed tomography (CT) was performed considering COVID-19 pandemic, and showed ground-glass opacities with consolidation in the dorsal segment of the right lower lung field. Then, qualitative real-time reverse-transcriptase-polymerase-chain-reaction (RT-PCR) was carried out and turned out to be positive. She was diagnosed right femoral intertrochanteric fracture with concomitant COVID-19 infection. Conservative treatment was applied to the fracture due to infection. After admission, fever and oxygen demand occurred but she recovered from COVID-19. Throughout the treatment period, no cross-infection from the patient was identified in our hospital. Conclusion: This case highlights the importance of considering chest CT as an effective screening method for infection on hospital admission in COVID-19-affected areas, especially in trauma patients with early asymptomatic novel COVID-19.
• Evaluation of Residual Human-Induced Pluripotent Stem Cells in Human Chondrocytes by Cell Type-Specific Glycosphingolipid Glycome Analysis Based on the Aminolysis-SALSA Technique.

  Takuji Miyazaki, Hisatoshi Hanamatsu, Liang Xu, Tomohiro Onodera, Jun-Ichi Furukawa, Kentaro Homan, Rikiya Baba, Toshisuke Kawasaki, Norimasa Iwasaki

  International journal of molecular sciences 21 1 2019年12月28日 [査読有り][通常論文]
   

  Cartilage damage may eventually lead to osteoarthritis because it is difficult to repair. Human-induced pluripotent stem cell (iPSC)-derived chondrocytes may potentially be used to treat cartilage damage, but the tumorigenicity of iPSCs is a major concern for their application in regenerative medicine. Many glycoconjugates serve as stem cell markers, and glycosphingolipids (GSLs) including H type 1 antigen (Fucα1-2Galβ1-3GlcNAc) have been expressed on the surface of iPSCs. The purpose of the present study was to investigate whether GSL-glycome analysis is useful for quality control of residual iPSCs in chondrocytes. We performed GSL-glycome analysis of undifferentiated iPSCs in chondrocytes by combining glycoblotting and aminolysis-sialic acid linkage-specific alkylamidation (SALSA) method, enabling the detection of small quantities of iPSC-specific GSL-glycans from 5 × 104 cells. Furthermore, we estimated the residual amount of iPSCs using R-17F antibody, which possesses cytotoxic activity toward iPSCs that is dependent on the Lacto-N-fucopentaose I (LNFP I) of GSL. Moreover, we could detect a small number of LNFP I during mesenchymal stem cells (MSCs) differentiation from iPSCs. This is the first demonstration that GSL-glycome analysis is useful for detecting undifferentiated iPSCs, and can thereby support safe regenerative medicine.
• Impact of Surgical Timing on Clinical Outcomes in Anatomic Double-Bundle Anterior Cruciate Ligament Reconstruction Using Hamstring Tendon Autografts.

  Rikiya Baba, Eiji Kondo, Koji Iwasaki, Zenta Joutoku, Jun Onodera, Tomohiro Onodera, Tomonori Yagi, Norimasa Iwasaki, Kazunori Yasuda

  Orthopaedic journal of sports medicine 7 11 2325967119880553 - 2325967119880553 2019年11月 

  Background: To date, no studies have analyzed the influence on clinical outcomes of the interval between an anterior cruciate ligament (ACL) injury and double-bundle (DB) reconstruction with hamstring tendon autografts. Hypotheses: (1) Performing ACL reconstruction sooner after an injury will reduce postoperative anterior and rotatory knee instability, (2) postoperative range of knee motion or functional results will not be affected by different intervals between injury and surgery, and (3) preoperative isokinetic peak torque of the quadriceps and hamstring muscles will be lower in patients undergoing surgery earlier, while postoperative muscle strength will not be affected by surgery timing. Study Design: Cohort study; Level of evidence, 3. Methods: This study was conducted on a total of 171 patients who had undergone anatomic DB ACL reconstruction with hamstring tendon autografts. The patients were divided into 3 groups based on the time to surgery: (1) ≤1 month after the injury (group E; n = 25), (2) between 1 and 3 months after the injury (group M; n = 72), and (3) >3 months after the injury (group D; n = 74). Patients were assessed for a minimum of 2 years after surgery. Results: Concerning postoperative anterior laxity, 1-way analysis of variance demonstrated a significant difference (P = .0274) among the 3 groups. Anterior laxity was significantly less in group E than in group D (P = .0206). Spearman rank correlation analysis showed a significant correlation (ρ = 0.200; P = .0327) between anterior knee laxity and time to surgery. Also, a significant correlation (P = .0461) was found between the degree of the pivot-shift phenomenon and time to surgery. There were no significant differences in loss of knee extension or flexion among the 3 groups, nor were there any differences in the Lysholm knee score or International Knee Documentation Committee grade. Postoperatively, there were no significant differences in peak torque of the quadriceps or hamstring muscles among the 3 groups. Conclusion: Early DB reconstruction led to significantly less anterior laxity compared with delayed reconstruction. There were no significant differences in postoperative range of knee motion or functional results among the 3 time intervals between injury and surgery in this study.
• 【4疾患からみる膠原病のいま】小児線状強皮症に対してシクロスポリン内服が有効であった2例

  眞井 翔子, 藤田 靖幸, 夏賀 健, 西江 渉, 小野寺 智洋, 岡 敏明, 清水 宏

  皮膚科の臨床 61 12 1797 - 1802 金原出版(株) 2019年11月 [査読有り][通常論文]
   

  ＜文献概要＞線状強皮症は皮膚の硬化と萎縮によって,整容面の問題だけではなく不可逆的な筋や関節の拘縮をきたしうる。小児発症が多く,治療効果と副作用の点から治療の選択に苦慮することが多い。われわれは,小児の四肢に生じた線状強皮症2例に対してシクロスポリン内服療法を行ったところ,1例は症状の改善を認め,1例では進行を抑制することができた。ともに経過中に重大な有害事象を生じず,小児のシクロスポリン内服療法は治療の選択肢として有力であると考えた。
• Assessment of valgus laxity after release of the medial structure in medial open-wedge high tibial osteotomy: an in vivo biomechanical study using quantitative valgus stress radiography.

  Dai Sato, Eiji Kondo, Koji Yabuuchi, Jun Onodera, Tomohiro Onodera, Tomonori Yagi, Keita Sakamoto, Akira Takasawa, Norimasa Iwasaki, Kazunori Yasuda

  BMC musculoskeletal disorders 20 1 481 - 481 2019年10月27日 

  BACKGROUND: To perform medial open-wedge high tibial osteotomy (OWHTO), surgeons expose the medial-proximal tibia by releasing or cutting the superficial layer of the medial collateral ligament (sMCL). Biomechanically, the sMCL provides primary restraint against valgus forces. Therefore, any release of the sMCL can cause valgus instability of the knee joint. The purpose of this study was to assess valgus laxity after release of the medial structure of the knee during OWHTO. METHODS: Between 2009 and 2015, 84 consecutive patients (93 knees) who underwent OWHTO using a locking plate were enrolled in this study. All patients underwent radiological examinations before surgery, during surgery, 1 year after surgery, and after plate removal to objectively assess valgus laxity. The medial joint space (MJS) and the joint line convergence angle (JLCA) of the knee were evaluated using quantitative valgus stress radiography. Clinical evaluation was performed 2 years after surgery. RESULTS: The mean functional knee score improved significantly, from 65.5 to 91.1 points (p < 0.0001). The mechanical axis percentage shifted to pass through a point 69.7% lateral from the medial edge of the tibial plateau. The MJS and JLCA increased significantly during OWHTO surgery (11.0 mm, 7.4 °, p < 0.0001). However, no significant differences were noted in the MJS and JLCA among preoperative, 1-year postoperative periods and after plate removal. CONCLUSION: Valgus laxity was significantly greater after release of the sMCL. However, no significant differences were noted in valgus laxity in preoperative, 1-year postoperative periods and after plate removal. Complete release of the sMCL did not cause postoperative valgus laxity after OWHTO surgery. TRIAL REGISTRATION: Trial registration number: No.012-0360.
• Depletion of glycosphingolipids induces excessive response of chondrocytes under mechanical stress.

  Shinji Matsubara, Tomohiro Onodera, Eijiro Maeda, Daisuke Momma, Masatake Matsuoka, Kentaro Homan, Toshiro Ohashi, Norimasa Iwasaki

  Journal of biomechanics 94 22 - 30 2019年09月20日 [査読有り][通常論文]
   

  Glycosphingolipids (GSLs) are ubiquitous membrane components that play an indispensable role in maintaining chondrocyte homeostasis. To gain better insight into roles of GSLs, we studied the effects of GSL-deletion on the physiological responses of chondrocytes to mechanical stress. Mice lacking Ugcg gene (Ugcg-/-) were genetically generated to obtain GSL-deficient mice, and their chondrocytes from the joints were used for functional analyses in vitro culture experiments. The cells were seeded in a three-dimensional collagen gel and subjected to 5%, 10% or 16% cyclic tensile strain for either 3 or 24 h. The gene expressions of chondrocyte anabolic and catabolic factors, and the induction of Ca2+ signaling were analyzed. Our results revealed that chondrocytes derived from GSL-deficient mice exhibited an elevation in the expression of catabolic factors (ADAMTS-5, MMP-13) following the exposure to strain with amplitudes of 10%. Likewise, applying cyclic tensile strain with these amplitudes resulted in an increased Ca2+ oscillation ratio in chondrocytes from GSL-deficient as compared to the ratio from control mice. These results demonstrated that deletion of GSL stimulated the catabolic responses of chondrocytes to mechanical stress via the augmentation of the sensitivity to mechanical stress that may lead to the cartilage deterioration. These findings suggest that the regulation of the physiological responses of chondrocytes by GSLs could be a potential target in a therapeutic intervention in osteoarthritis.
• 軟骨破片刺激モデルにおいて軟骨細胞は軟骨内骨化関連の遺伝子発現が上昇する

  江畑 拓, 濱崎 雅成, Terkawi M. Alaa, 小野寺 智洋, 松前 元, 田 園, Alhasan Hend, 宮崎 拓自, 徐 亮, 菱村 亮介, 岩崎 倫政

  日本整形外科学会雑誌 93 8 S1688 - S1688 (公社)日本整形外科学会 2019年09月
• インプラントの破片によって引き起こされる炎症性骨溶解の発生におけるケモカインXCL1/リンホタクチンの関与

  田 園, テルカウイ・アラー, 小野寺 智洋, アルハサン・ヘンド, 松前 元, 高橋 大介, 濱崎 雅成, 江畑 拓, 岩崎 倫政

  日本骨代謝学会学術集会プログラム抄録集 37回 183 - 183 2019年09月
• Alteration of the Total Cellular Glycome during Late Differentiation of Chondrocytes.

  Kentaro Homan, Hisatoshi Hanamatsu, Jun-Ichi Furukawa, Kazue Okada, Ikuko Yokota, Tomohiro Onodera, Norimasa Iwasaki

  International journal of molecular sciences 20 14 2019年07月19日 [査読有り][通常論文]
   

  In normal articular cartilage, chondrocytes do not readily proliferate or terminally differentiate, and exhibit a low level of metabolism. Hypertrophy-like changes of chondrocytes have been proposed to play a role in the pathogenesis of osteoarthritis by inducing protease-mediated cartilage degradation and calcification; however, the molecular mechanisms underlying these changes are unclear. Glycans are located on the outermost cell surface. Dynamic cellular differentiation can be monitored and quantitatively characterized by profiling the glycan structures of total cellular glycoproteins. This study aimed to clarify the alterations in glycans upon late differentiation of chondrocytes, during which hypertrophy-like changes occur. Primary mouse chondrocytes were differentiated using an insulin-induced chondro-osteogenic differentiation model. Comprehensive glycomics, including N-glycans, O-glycans, free oligosaccharides, glycosaminoglycan, and glycosphingolipid, were analyzed for the chondrocytes after 0-, 10- and 20-days cultivation. The comparison and clustering of the alteration of glycans upon hypertrophy-like changes of primary chondrocytes were performed. Comprehensive glycomic analyses provided complementary alterations in the levels of various glycans derived from glycoconjugates during hypertrophic differentiation. In addition, expression of genes related to glycan biosynthesis and metabolic processes was significantly correlated with glycan alterations. Our results indicate that total cellular glycan alterations are closely associated with chondrocyte hypertrophy and help to describe the glycophenotype by chondrocytes and their hypertrophic differentiation. our results will assist the identification of diagnostic and differentiation biomarkers in the future.
• Correction osteotomy for bilateral varus knee deformity caused by premature epiphyseal closure induced by hypervitaminosis A: a case report.

  Masatake Matsuoka, Tomohiro Onodera, Tokifumi Majima, Koji Iwasaki, Daisuke Takahashi, Eiji Kondo, Norimasa Iwasaki

  BMC musculoskeletal disorders 20 1 287 - 287 2019年06月15日 

  BACKGROUND: A vitamin A derivative, 13-cis-retinoic acid (isotretinoin), has been administered to treat several types of pediatric cancer and has improved survival rates in patients despite being known to induce premature epiphyseal closure. As the number of patients treated by 13-cis-retinoic acid increases, demands for salvage treatment after systemic retinoid therapy are emerging. However, few studies have described the surgical treatment of this disease. CASE PRESENTATION: We report a case with bilateral varus knee deformity due to premature epiphyseal closure that occurred during treatment with isotretinoin for neuroblastoma. The patient was successfully treated with correction osteotomy using a Taylor spatial frame in the right knee joint and femoral closed wedge osteotomy using a locking plate in left knee joint. Histopathological examination of the growth plate showed polar irregularity of chondrocytes and decreased cartilage matrix without apoptosis. In contrast, arthroscopic findings showed an intact joint surface. No recurrence of varus deformity was evident on follow-up at 1 year. CONCLUSIONS: To the best of our knowledge, this represents the first report of correction osteotomy for varus knee deformity due to premature epiphyseal closure that occurred during treatment with isotretinoin.
• ACL遺残組織温存の賛否 解剖学的2束前十字靱帯再建術後の骨孔拡大における遺残組織温存の効果

  近藤 英司, 舛田 哲朗, 小野寺 純, 小野寺 智洋, 岩崎 浩司, 井上 雅之, 岩崎 倫政, 八木 知徳, 安田 和則

  JOSKAS 44 4 564 - 564 (一社)日本関節鏡・膝・スポーツ整形外科学会 2019年05月
• Chondrogenic differentiation of mouse induced pluripotent stem cells using the three-dimensional culture with ultra-purified alginate gel.

  Kazutoshi Hontani, Tomohiro Onodera, Michiyo Terashima, Daisuke Momma, Masatake Matsuoka, Rikiya Baba, Zenta Joutoku, Shinji Matsubara, Kentaro Homan, Ryosuke Hishimura, Liang Xu, Norimasa Iwasaki

  Journal of biomedical materials research. Part A 107 5 1086 - 1093 2019年05月 [査読有り][通常論文]
   

  As articular cartilages have rarely healed by themselves because of their characteristics of avascularity and low cell density, surgical intervention is ideal for patients with cartilaginous injuries. Because of structural characteristics of the cartilage tissue, a three-dimensional culture of stem cells in biomaterials is a favorable system on cartilage tissue engineering. Induced pluripotent stem cells (iPSCs) are a new cell source in cartilage tissue engineering for its characteristics of self-renewal capability and pluripotency. However, the optimal cultivation condition for chondrogenesis of iPSCs is still unknown. Here we show that a novel chondrogenic differentiation method of iPSCs using the combination of three-dimensional cultivation in ultra-purified alginate gel (UPAL gel) and multi-step differentiation via mesenchymal stem cell-like cells (iPS-MSCs) could efficiently and specifically differentiate iPSCs into chondrocytes. The iPS-MSCs in UPAL gel culture sequentially enhanced the expression of chondrogenic marker without the upregulation of that of osteogenic and adipogenic marker and histologically showed homogeneous chondrogenic extracellular matrix formation. Our results suggest that the pluripotency of iPSCs can be controlled when iPSCs are differentiated into iPS-MSCs before embedding in UPAL gel. These results lead to the establishment of an efficient three-dimensional system to engineer artificial cartilage tissue from iPSCs for cartilage regeneration. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1086-1093, 2019.
• 遺残組織温存解剖学的二束前十字靱帯再建術の再鏡視所見 非温存群との年齢別検討

  江畑 拓, 近藤 英司, 岩崎 浩司, 珍部 正嗣, 小野寺 智洋, 小野寺 純, 安田 和則, 八木 知徳, 岩崎 倫政

  JOSKAS 44 2 426 - 427 (一社)日本関節鏡・膝・スポーツ整形外科学会 2019年04月 

  遺残組織温存二束ACL再建術(R法)と従来の二束再建術(D法)の再鏡視所見を年齢別に比較した。片側ACL損傷に対して膝屈筋腱ハイブリッド代用材料を用いて解剖学的二束ACL再建を行い、術後1年で再鏡視を行った171例を対象とした。遺残組織の連続性を認めないType IVの症例に対してはD法(89例、平均28歳)を、遺残組織の連続性があるType IからIIIの症例に対してはR法(82例、平均30歳)を行った。それぞれを24歳以下のY群、25歳以上39歳以下のM群、40歳以上のO群の三つの年齢群に分け、術後1年経過時の臨床成績および再鏡視所見を年齢別に評価した。30°屈曲位における膝前方不安定性の対健側差について各年齢群で比較したところ、両術式間に有意な差はなかった。また、Lysholm scoreおよびIKDC評価に関しても同様に両術式間で有意差を認めなかった。各術式の年齢群間の比較では、D法においてO群はY群と比べ有意に不良であったが、R法では3群間に有意な差はなかった。
• CCL21/CCR7 axis regulating juvenile cartilage repair can enhance cartilage healing in adults.

  Zenta Joutoku, Tomohiro Onodera, Masatake Matsuoka, Kentaro Homan, Daisuke Momma, Rikiya Baba, Kazutoshi Hontani, Masanari Hamasaki, Shinji Matsubara, Ryosuke Hishimura, Norimasa Iwasaki

  Scientific reports 9 1 5165 - 5165 2019年03月26日 [査読有り][通常論文]
   

  Juvenile tissue healing is capable of extensive scarless healing that is distinct from the scar-forming process of the adult healing response. Although many growth factors can be found in the juvenile healing process, the molecular mechanisms of juvenile tissue healing are poorly understood. Here we show that juvenile mice deficient in the chemokine receptor CCR7 exhibit diminished large-scale healing potential, whereas CCR7-depleted adult mice undergo normal scar-forming healing similar to wild type mice. In addition, the CCR7 ligand CCL21 was transiently expressed around damaged cartilage in juvenile mice, whereas it is rarely expressed in adults. Notably, exogenous CCL21 administration to adults decreased scar-forming healing and enhanced hyaline-cartilage repair in rabbit osteochondral defects. Our data indicate that the CCL21/CCR7 axis may play a role in the molecular control mechanism of juvenile cartilage repair, raising the possibility that agents modulating the production of CCL21 in vivo can improve the quality of cartilage repair in adults. Such a strategy may prevent post-traumatic arthritis by mimicking the self-repair in juvenile individuals.
• Identification of IL-27 as potent regulator of inflammatory osteolysis associated with vitamin E-blended ultra-high molecular weight polyethylene debris of orthopedic implants

  Terkawi MA, Kadoya K, Takahashi D, Tian Y, Hamasaki M, Matsumae G, Alhasan H, Elmorsy S, Uetsuki K, Onodera T, Takahata M, Iwasaki N

  Acta Biomaterialia 89 242 - 251 2019年03月 [査読有り][通常論文]
   

  Vitamin E-blended ultra-high molecular weight polyethylene (VE-UHMWPE) is a newly introduced material for prosthetic components that has proven a better mechanical performance with lesser adverse cellular responses than conventional polyethylene in experimental animal models. However, the mechanisms by which VE-UHMWPE particles trigger a reduced osteolytic activity are unclear and remain to be investigated. Therefore, the current study aims at exploring a possible anti-osteolytic mechanism associated with VE-UHMWPE particles. Transcriptional profiling and bioinformatic analyses of human macrophages stimulated by VE-UHMWPE particles revealed a distinct transcriptional program from macrophages stimulated with UHMWPE particles. Out of the up-regulated genes, IL-27 was found to be significantly elevated in macrophages cultured with VE-UHMWPE particles as compared to these with UHMWPE particles (p = 0.0084). Furthermore, we studied the potential anti-osteolytic function of IL-27 in osteolysis murine model. Interestingly, administration of recombinant IL-27 onto calvariae significantly alleviated osteolytic lesions triggered by UHMWPE particles (p = 0.0002). Likewise, IL-27 inhibited differentiation of osteoclasts (p = 0.0116) and reduced inflammatory response (p < 0.0001) elicited by conventional UHMWPE particles in vitro. This is the first study demonstrating the involvement of IL-27 in macrophage response to VE-UHMWPE particles and its regulatory role in osteolysis. Our data highlight a novel therapeutic agent for treatment of inflammatory osteolysis induced by polyethylene debris. STATEMENT OF SIGNIFICANCE: Aseptic loosening due to inflammatory osteolysis remains the major cause of arthroplasty failure and represents a substantial economic burden worldwide. Ideal approach to prevent this failure should be directed to minimize inflammatory response triggered by wear particles at the site of implant. Understanding the mechanism by which VE-UHMWPE particles triggers lesser cellular responses and reduced osteolysis as compared to conventional UHMWPE particles may aid in discovery of regulatory factors. In the current study, we reported that IL-27 is a potent regulator of inflammatory osteolysis involved in the reduced biologic activities and osteolytic potentials associated with VE-UHMWPE particles. Initiating the production IL-27 in vivo after total joint arthroplasties might be a novel strategy to prolong the life-spam of implant.
• Effects of Ultra-Purified Alginate Gel Implantation on Meniscal Defects in Rabbits.

  WooYoung Kim, Tomohiro Onodera, Eiji Kondo, Yasuyuki Kawaguchi, Mohamad Alaa Terkawi, Rikiya Baba, Kazutoshi Hontani, Zenta Joutoku, Shinji Matsubara, Kentaro Homan, Ryosuke Hishimura, Norimasa Iwasaki

  The American journal of sports medicine 47 3 640 - 650 2019年03月 [査読有り][通常論文]
   

  BACKGROUND: Many tissue-engineered methods for meniscal repair have been studied, but their utility remains unclear. HYPOTHESIS: Implantation of low-endotoxin, ultra-purified alginate (UPAL) gel without cells could induce fibrocartilage regeneration on meniscal defects in rabbits. STUDY DESIGN: Controlled laboratory study. METHODS: Forty-two mature Japanese White rabbits were divided into 2 groups of 21 animals each. In each animal, a cylindrical defect measuring 2 mm in diameter was created with a biopsy punch on the anterior horn of the medial meniscus. In the control group, no treatment was applied on the left medial meniscal defect. In the UPAL gel group, the right medial meniscal defect was injected with the UPAL gel and gelated by a CaCl2 solution. Samples were evaluated at 3, 6, and 12 weeks postoperatively. For biomechanical evaluation, 6 additional samples from intact animals were used for comparison. RESULTS: The macroscopic score was significantly greater in the UPAL gel group than in the control group at 3 weeks (mean ± SE: 5.6 ± 0.82 vs 3.4 ± 0.83, P = .010), 6 weeks (5.9 ± 0.72 vs 2.5 ± 0.75, P = .026), and 12 weeks (5.2 ± 1.21 vs 1.0 ± 0.63, P = .020). The histological score was significantly greater in the UPAL group than in the control group at 3 weeks (2.1 ± 0.31 vs 1.2 ± 0.25, P = .029) and 12 weeks (2.2 ± 0.55 vs 0.3 ± 0.21, P = .016). The mean stiffness of the reparative tissue in the UPAL gel group was significantly greater than that in the control group at 6 weeks (24.325 ± 3.920 N/mm vs 8.723 ± 1.190 N/mm, P = .006) and at 12 weeks (27.804 ± 6.169 N/mm vs not applicable [because of rupture]). CONCLUSION: The UPAL gel enhanced the spontaneous repair of fibrocartilage tissues in a cylindrical meniscal defect in rabbits. CLINICAL RELEVANCE: These results imply that the acellular UPAL gel may improve the repair of traumatic meniscal injuries.
• Preoperative radiographic and clinical factors associated with postoperative floating of the lesser toes after resection arthroplasty for rheumatoid forefoot deformity.

  Onodera T, Nakano H, Homan K, Kondo E, Iwasaki N

  BMC musculoskeletal disorders 20 1 87 - 87 2019年02月19日 [査読有り][通常論文]
   

  BACKGROUND: This study aimed to clarify the characteristics associated with postoperative floating of the lesser toes, especially focusing on the medial and lateral lessor toes, after arthrodesis of the first metatarsophalangeal joint and resection arthroplasty of the lessor toes in rheumatoid forefoot deformity. METHODS: Fourty-seven feet of 43 people who underwent resection arthroplasty of the metatarsal head of the lesser toes for rheumatoid arthritis of the metatarsophalangeal joints were included. We retrospectively evaluated the preoperative radiographic findings and clinical characteristics of the patients, and the occurrence of postoperative floating of the lesser toes. The mean duration of follow-up was 36.5 (range 12 to 114) months. RESULTS: Preoperative dislocation grades of the second and third toes that demonstrated postoperative floating were significantly higher than those of toes that did not experience postoperative floating. The hallux valgus deformity before surgery was significantly more severe in toes with postoperative floating of the second and third lessor toes than those with no floating (p < 0.05). In addition, the Japanese Society for Surgery of the Foot (JSSF) hallux scale scores before surgery in toes with postoperative floating of the fourth and fifth lessor toes were significantly worse than those in non-dislocating toes (p < 0.05). CONCLUSIONS: The preoperative condition of the first metatarsophalangeal joint, including hallux valgus deformity, pain, range of motion, activity of daily living, and function is significantly different between postoperative floating of the lesser toes and non-floating of them after resection arthroplasty for rheumatoid forefoot deformity.
• 軟骨破片刺激モデルにおいて軟骨細胞は軟骨内骨化関連の遺伝子発現が上昇する

  江畑 拓, 濱崎 雅成, アラー・テルカウイ, 小野寺 智洋, 松前 元, 田 園, ヘンド・アルハサン, 宮崎 拓自, 徐 亮, 細川 吉暁, 岩崎 倫政

  北海道整形災害外科学会雑誌 61 137th suppl 35 - 35 北海道整形災害外科学会 2019年
• 骨軟骨欠損に対する骨髄穿刺濃縮細胞と高純度アルギン酸ゲル併用移植の検討

  徐 亮, 瓜田 淳, 小野寺 智洋, 菱村 亮介, 濱崎 雅成, 梁 大偉, 宝満 健太郎, 岩崎 倫政

  北海道整形災害外科学会雑誌 61 137th suppl 13 - 13 北海道整形災害外科学会 2019年
• 軟骨再生医療への応用を標的とした未分化iPS細胞特異的除去法の検討

  宮崎 拓自, 小野寺 智洋, 徐 亮, 宝満 健太郎, 濱崎 雅成, 岩崎 倫政, 花松 久寿, 古川 潤一, 川嵜 敏祐

  北海道整形災害外科学会雑誌 61 137th suppl 34 - 34 北海道整形災害外科学会 2019年
• 両先天性内反母趾に対し両第I中足骨外反矯正骨切り術を施行した1例

  徳廣 泰貴, 小野寺 智洋, 上徳 善太, 岩崎 浩司, 新井 隆太, 岩崎 倫政, 近藤 英司

  北海道整形災害外科学会雑誌 61 136th suppl 36 - 36 北海道整形災害外科学会 2019年
• 膝十字靱帯不全を合併した内側型変形性膝関節症に対する膝十字靱帯再建術と高位脛骨骨切り術の二期的手術の短期成績

  清水 寛和, 岩崎 浩司, 近藤 英司, 小野寺 智洋, 新井 隆太, 上徳 善太, 岩崎 倫政

  北海道整形災害外科学会雑誌 61 137th suppl 23 - 23 北海道整形災害外科学会 2019年
• A Novel Cartilage Fragments Stimulation Model Revealed that Macrophage Inflammatory Response Causes an Upregulation of Catabolic Factors of Chondrocytes In Vitro.

  Hamasaki M, Terkawi MA, Onodera T, Homan K, Iwasaki N

  Cartilage 12 1947603519828426 354 - 361 2019年01月 [査読有り][通常論文]
   

  OBJECTIVE: Osteoarthritis is a progressive joint disease characterized by cartilage degradation and synovial inflammation. Presence of cartilage fragments in the joint due to degradation of cartilage is thought to be associated with local inflammatory response and progressive osteoarthritic process. Understanding the mechanism by which cartilage fragments elicit this destructive process should aid in designing novel therapeutic approaches. Therefore, objective of current study is to establish an in vitro model to examine the cross-talk between chondrocytes and cartilage fragments-stimulated macrophages. DESIGN: Cartilage fragments were prepared from femoral head cartilages of mice and analyzed using a scanning electron microscope and particle size analyzer. Bone marrow-derived macrophages were co-cultured with cartilage fragments and chondrocytes using transwell co-culture system. Macrophage inflammatory mediators in supernatant of cultures were determined by ELISA and gene expression of macrophages and chondrocyte were quantified by qRT-PCR. RESULTS: Shapes of cartilage fragments were irregular with sizes ranged between 0.54 and 55 μm. Macrophages cultured with cartilage fragments released significantly higher concentrations of TNF-α, IL-6, and NO than those of mock and control. Consistently, gene expressions of TNF-α, IL-6, and MMP-9 were significantly increased in stimulated macrophages. The elevation in production of pro-inflammatory molecules in stimulated macrophages cultures were coincident with an increase in gene expression of chondrocyte MMP-13, iNOS, and IL-6. CONCLUSION: We developed an in vitro co-culture model to study the impact of stimulation of macrophage by cartilage fragments on the expression of chondrocyte carbolic factors. Our results revealed that cartilage fragments triggered macrophages inflammatory response that enhanced the production of chondrocyte catabolic factors.
• Osteochondral Autograft Transplantation Technique Augmented by an Ultrapurified Alginate Gel Enhances Osteochondral Repair in a Rabbit Model

  Ryosuke Hishimura, Tomohiro Onodera, Kazutoshi Hontani, Rikiya Baba, Kentaro Homan, Shinji Matsubara, Zenta Joutoku, WooYoung Kim, Takayuki Nonoyama, Takayuki Kurokawa, Jian Ping Gong, Norimasa Iwasaki

  American Journal of Sports Medicine 47 2 468 - 478 2019年01月 [査読有り][通常論文]
  
• Coordinated existence of multiple gangliosides is required for cartilage metabolism.

  Momma D, Onodera T, Homan K, Matsubara S, Sasazawa F, Furukawa J, Matsuoka M, Yamashita T, Iwasaki N

  Osteoarthritis Cartilage 2018年11月 [査読有り][通常論文]
  
• マウスiPS細胞からの軟骨分化誘導と糖鎖解析を用いた新規バイオマーカーの探索

  本谷 和俊, 小野寺 智洋, 寺島 理代, 門間 太輔, 松岡 正剛, 馬場 力哉, 上徳 善太, 松原 新史, 宝満 健太郎, 菱村 亮介, 金 佑泳, 濱崎 雅成, 徐 亮, 岩崎 倫政

  北海道整形災害外科学会雑誌 60 1 145 - 145 北海道整形災害外科学会 2018年08月
• 軟骨細胞の力学的ストレス応答におけるスフィンゴ糖脂質の機能解析

  松原 新史, 小野寺 智洋, 門間 太輔, 馬場 力哉, 本谷 和俊, 上徳 善太, 宝満 健太郎, 岩崎 倫政, 大橋 俊朗, 前田 英次郎

  北海道整形災害外科学会雑誌 60 1 145 - 146 北海道整形災害外科学会 2018年08月
• 自家骨軟骨柱移植術に高純度アルギン酸ゲルを併用した治療効果の検討

  菱村 亮介, 小野寺 智洋, 上徳 善太, 宝満 健太郎, 松原 新史, 金 佑泳, 濱崎 雅成, 岩崎 倫政

  日本整形外科学会雑誌 92 8 S1639 - S1639 (公社)日本整形外科学会 2018年08月
• 軟骨変性と肥大分化における軟骨細胞グライコームの類似性

  宝満 健太郎, 小野寺 智洋, 花松 久寿, 古川 潤一, 上徳 善太, 松原 新史, 菱村 亮介, 金 佑泳, 濱崎 雅成, 宮崎 拓自, 岩崎 倫政

  日本整形外科学会雑誌 92 8 S1653 - S1653 (公社)日本整形外科学会 2018年08月
• マクロファージ共培養モデルにおいて軟骨破片が引き起こすマクロファージの炎症反応および軟骨細胞への影響

  濱崎 雅成, Terkawi Mohamad Alaa, 小野寺 智洋, 宝満 健太郎, 上徳 善太, 松原 新史, 菱村 亮介, 徐 亮, 宮崎 拓自, 田 園, 岩崎 倫政

  日本整形外科学会雑誌 92 8 S2012 - S2012 (公社)日本整形外科学会 2018年08月
• 膝複合靱帯損傷を合併した脛骨高原骨折術後下肢外反変形に対してDouble Level Osteotomyを行った1例

  下田 康平, 近藤 英司, 珍部 正嗣, 小野寺 智洋, 新井 隆太, 岩崎 浩司, 岩崎 倫政

  北海道整形災害外科学会雑誌 60 1 109 - 115 北海道整形災害外科学会 2018年08月 [査読無し][通常論文]
   

  右脛骨高原骨折術後変形治癒に対して自家腸骨移植術、複合靱帯再建術、およびDouble level osteotomy(DLO)を行った症例を経験した。症例は33歳男性。3年前に交通事故で受傷。右脛骨高原骨折に対し他院にて骨接合術が行われたが、術後の変形治癒、膝内側および後方不安定性のため当科紹介となった。初診時、右脛骨外側関節面の著しい陥没と右膝外反および後方不安定性を認めた。脛骨関節面再建のため抜釘および自家腸骨移植術を行い、2期的に1束後十字靱帯再建と、内側側副靱帯浅層および後斜走靱帯再建を行った。しかしながら徐々に外反変形が進行、下肢機能軸は125%、Medial proximal tibial angle(MPTA)が97°であったため、関節面傾斜を考慮に、大腿骨および脛骨の内側楔状閉鎖式DLOを行った。術後6ヵ月で、関節可動域は0〜130°、膝前後・外反不安定性の左右差は認めず、単純X線にて下肢機能軸が35%、MPTA85°、Lateral distal femoral angle 89°となった。JOA scoreは80点である。今後長期の経過観察の必要がある。(著者抄録)
• Bone Marrow Stimulation Technique Augmented by an Ultrapurified Alginate Gel Enhances Cartilage Repair in a Canine Model.

  Rikiya Baba, Tomohiro Onodera, Masatake Matsuoka, Kazutoshi Hontani, Zenta Joutoku, Shinji Matsubara, Kentaro Homan, Norimasa Iwasaki

  The American journal of sports medicine 46 8 1970 - 1979 2018年07月 [査読有り][通常論文]
   

  BACKGROUND: The optimal treatment for a medium- or large-sized cartilage lesion is still controversial. Since an ultrapurified alginate (UPAL) gel enhances cartilage repair in animal models, this material is expected to improve the efficacy of the current treatment strategies for cartilage lesions. HYPOTHESIS: The bone marrow stimulation technique (BMST) augmented by UPAL gel can induce hyaline-like cartilage repair. STUDY DESIGN: Controlled laboratory study. METHODS: Two cylindrical osteochondral defects were created in the patellar groove of 27 beagle dogs. A total of 108 defects were divided into 3 groups: defects without intervention (control group), defects with the BMST (microfracture group), and defects with the BMST augmented by implantation of UPAL gel (combined group). At 27 weeks postoperatively, macroscopic and histological evaluations, micro-computed tomography assessment, and mechanical testing were performed for each reparative tissue. RESULTS: The defects in the combined group were almost fully covered with translucent reparative tissues, which consisted of hyaline-like cartilage with well-organized collagen structures. The macroscopic score was significantly better in the combined group than in the control group ( P < .05). The histological scores in the combined group were significantly better than those in the control group ( P < .01) and microfracture group ( P < .05). Although the repaired subchondral bone volumes were not influenced by UPAL gel augmentation, the mechanical properties of the combined group were significantly better than those of the microfracture group ( P < .05). CONCLUSION: The BMST augmented by UPAL gel elicited hyaline-like cartilage repair that had characteristics of rich glycosaminoglycan and matrix immunostained by type II collagen antibody in a canine osteochondral defect model. The present results suggest that the current technique has the potential to be one of the autologous matrix-induced chondrogenesis techniques of the future and to expand the operative indications for the BMST without loss of its technical simplicity. CLINICAL RELEVANCE: The data support the clinical reality of 1-step minimally invasive cartilage-reparative medicine with UPAL gel without harvesting donor cells.
• Double-Bundle Anterior Cruciate Ligament Reconstruction Using Hamstring Tendon Hybrid Grafts in Patients Over 40 Years of Age: Comparisons Between Different Age Groups

  Yusuke Nishio, Eiji Kondo, Jun Onodera, Tomohiro Onodera, Tomonori Yagi, Norimasa Iwasaki, Kazunori Yasuda

  Orthopaedic Journal of Sports Medicine 6 5 2325967118773685  2018年05月21日 [査読有り][通常論文]
   

  Background: Several recent studies have reported that favorable clinical results and a high level of patient satisfaction can generally be obtained with no increased risk of complications after single-bundle anterior cruciate ligament (ACL) reconstruction performed in patients > 40 years of age. However, no studies have yet clarified the age-based differences in clinical outcomes after double-bundle reconstruction. Purpose: To compare clinical outcomes after double-bundle ACL reconstruction using hamstring tendon hybrid grafts between patients in 2 different age groups: ≥40 years and < 40 years. Study Design: Cohort study Level of evidence, 3. Methods: A retrospective study was conducted using 96 patients (48 men, 48 women mean age, 37 years) who underwent unilateral ACL reconstruction between 2008 and 2011. These patients were divided into 2 groups: group M included patients ≥40 years of age (n = 40 patients), and group Y included patients < 40 years of age (n = 56 patients). All patients underwent the same anatomic double-bundle ACL reconstruction procedure. Clinical outcomes were evaluated at 2 years after surgery. Tunnel enlargement was also evaluated by computed digital radiography at 1 week and 2 years after surgery. Results: Mean postoperative side-to-side differences in anterior laxity were 0.5 ± 1.9 mm and 1.2 ± 1.5 mm in groups M and Y, respectively there was a significant difference between the 2 groups (P =.039). There were no significant differences between the groups in Lysholm knee scores, International Knee Documentation Committee (IKDC) scores, or peak muscle torque of the hamstring. On the other hand, peak muscle torque of the quadriceps was significantly lower in group M (81%) than in group Y (89%) (P =.025). With respect to femoral tunnel enlargement, the posterolateral tunnel in group M was significantly larger than that in group Y on anteroposterior and lateral radiographs (P =.015 and P =.002, respectively). Conclusion: Equivalent clinical outcomes were seen between the 2 age groups after double-bundle ACL reconstruction. Postoperative anterior laxity was significantly less in older patients than in younger patients, however, older patients had significantly less quadriceps muscle strength than younger patients. Surgeons should be aware of residual muscle weakness and tunnel enlargement when performing double-bundle ACL reconstruction in older patients.
• 膝屈筋腱ハイブリッド代用材料を用いた遺残組織温存解剖学的2束前十字靱帯再建術の再鏡視所見 非温存群との年齢別の検討

  江畑 拓, 近藤 英司, 岩崎 浩司, 珍部 正嗣, 新井 隆太, 小野寺 智洋, 小野寺 純, 安田 和則, 八木 知徳, 岩崎 倫政

  JOSKAS 43 4 685 - 685 (一社)日本関節鏡・膝・スポーツ整形外科学会 2018年05月
• 膝屈筋腱ハイブリッド代用材料を用いた遺残組織温存解剖学的2束前十字靱帯再建術の臨床成績 非温存群との年齢別検討

  江畑 拓, 近藤 英司, 岩崎 浩司, 珍部 正嗣, 新井 隆太, 小野寺 智洋, 小野寺 純, 安田 和則, 八木 知徳, 岩崎 倫政

  日本整形外科学会雑誌 92 3 S617 - S617 (公社)日本整形外科学会 2018年03月
• 軟骨細胞肥大分化の包括的N型糖鎖プロファイリングによる糖鎖バイオマーカー探索

  宝満 健太郎, 小野寺 智洋, 花松 久寿, 古川 潤一, 門間 太輔, 松岡 正剛, 馬場 力哉, 本谷 和俊, 上徳 善太, 松原 新史, 菱村 亮介, 金 佑詠, 濱崎 雅成, 岩崎 倫政

  北海道整形災害外科学会雑誌 60 134th suppl 36 - 36 北海道整形災害外科学会 2018年
• マクロファージ共培養モデルにおいて軟骨破片が軟骨細胞に与える影響

  濱崎 雅成, テルカウイ・アラー, 小野寺 智洋, 宝満 健太郎, 上徳 善太, 松原 新史, 菱村 亮介, 徐 亮, 田 園, 岩崎 倫政

  北海道整形災害外科学会雑誌 60 134th suppl 37 - 37 北海道整形災害外科学会 2018年
• 自家骨軟骨柱移植術に高純度アルギン酸ゲルを併用した治療効果の検証

  菱村 亮介, 小野寺 智洋, 上徳 善太, 宝満 健太郎, 松原 新史, 金 佑泳, 濱崎 雅成, 岩崎 倫政

  北海道整形災害外科学会雑誌 60 135th suppl 22 - 22 北海道整形災害外科学会 2018年
• 膝屈筋腱ハイブリッド代用材料を用いた遺残組織温存解剖学的2束前十字靱帯再建術の臨床成績 非温存群との年齢別検討

  江畑 拓, 近藤 英司, 岩崎 浩司, 新井 隆太, 小野寺 智洋, 小野寺 純, 安田 和則, 八木 知徳, 岩崎 倫政

  北海道整形災害外科学会雑誌 60 135th suppl 24 - 24 北海道整形災害外科学会 2018年
• ビタミンA誘導体の長期投与による早期骨端線閉鎖により両膝内反変形を認めた一例

  長谷川 裕一, 小野寺 智洋, 岩崎 浩司, 珍部 正嗣, 新井 隆太, 近藤 英司, 岩崎 倫政

  北海道整形災害外科学会雑誌 60 135th suppl 63 - 63 北海道整形災害外科学会 2018年
• 両膝に発生した内側円板状半月板の1例

  青木 佑介, 珍部 正嗣, 近藤 英司, 岩崎 浩司, 新井 隆太, 小野寺 智洋, 安田 和則, 岩崎 倫政

  北海道整形災害外科学会雑誌 60 135th suppl 63 - 63 北海道整形災害外科学会 2018年
• 自家骨軟骨柱移植術に高純度アルギン酸ゲルを併用した治療効果の検証

  菱村 亮介, 小野寺 智洋, 上徳 善太, 宝満 健太郎, 松原 新史, 金 佑泳, 濱崎 雅成, 岩崎 倫政

  北海道整形災害外科学会雑誌 60 135th suppl 22 - 22 北海道整形災害外科学会 2018年
• Transcriptional profile of human macrophages stimulated by ultra-high molecular weight polyethylene particulate debris of orthopedic implants uncovers a common gene expression signature of rheumatoid arthritis

  Mohamad Alaa Terkawi, Masanari Hamasaki, Daisuke Takahashi, Masahiro Ota, Ken Kadoya, Tomoyo Yutani, Keita Uetsuki, Tsuyoshi Asano, Tohru Irie, Ryuta Arai, Tomohiro Onodera, Masahiko Takahata, Norimasa Iwasaki

  Acta Biomaterialia 65 417 - 425 2018年01月01日 [査読有り][通常論文]
   

  Osteolysis is a serious postoperative complication of total joint arthroplasty that leads to aseptic loosening and surgical revision. Osteolysis is a chronic destructive process that occurs when host macrophages recognize implant particles and release inflammatory mediators that increase bone-resorbing osteoclastic activity and attenuate bone-formation osteoblastic activity. Although much progress has been made in understanding the molecular responses of macrophages to implant particles, the pathways/signals that initiate osteolysis remain poorly characterized. Transcriptomics and gene-expression profiling of these macrophages may unravel key mechanisms in the pathogenesis of osteolysis and aid the identification of molecular candidates for therapeutic intervention. To this end, we analyzed the transcriptional profiling of macrophages exposed to ultra-high molecular weight polyethylene (UHMWPE) particles, the most common components used in bearing materials of orthopedic implants. Regulated genes in stimulated macrophages were involved in cytokine, chemokine, growth factor and receptor activities. Gene enrichment analysis suggested that stimulated macrophages elicited common gene expression signatures for inflammation and rheumatoid arthritis. Among the regulated genes, tumor necrosis factor superfamily member 15 (TNFSF15) and chemokine ligand 20 (CCL20) were further characterized as molecular targets involved in the pathogenesis of osteolysis. Treatment of monocyte cultures with TNFSF15 and CCL20 resulted in an increase in osteoclastogenesis and bone-resorbing osteoclastic activity, suggesting their potential contribution to loosening between implants and bone tissues. Statement of Significance Implant loosening due to osteolysis is the most common mode of arthroplasty failure and represents a great challenge to orthopedic surgeons and a significant economic burden for patients and healthcare services worldwide. Bone loss secondary to a local inflammatory response initiated by particulate debris from implants is considered the principal feature of the pathogenesis of osteolysis. In the present study, we analyzed the transcriptional profiling of human macrophages exposed to UHMWPE particles and identified a large number of inflammatory genes that were not identified previously in macrophage responses to wear particles. Our data provide a new insight into the molecular pathogenesis of osteolysis and highlights a number of molecular targets with prognostic and therapeutic implications.
• Quantitative knee cartilage measurement at MR imaging of patients with anterior cruciate ligament tear

  Kazuki Kato, Tamotsu Kamishima, Eiji Kondo, Tomohiro Onodera, Shota Ichikawa

  Radiological Physics and Technology 10 4 431 - 438 2017年12月01日 [査読有り][通常論文]
   

  In previous studies, numerous approaches were proposed that assess knee cartilage volume quantitatively using 3D magnetic resonance (MR) imaging. However, the clinical use of these approaches is limited because 3D MR is prone to metal artifacts in postoperative cases. Our purpose in this study was to validate a method for knee cartilage volume quantification using conventional MR imaging in patients who underwent anterior cruciate ligament (ACL) reconstruction surgery. The study included 16 patients who underwent MR imaging before and 1 year after ACL reconstruction surgery. Knee cartilage volumes were measured by our computer-based method with the use of T1-weighted sagittal images. We classified the cartilage into eight regions and made comparisons between preoperative and postoperative cartilage volumes in each region. There was a significant difference between preoperative and postoperative cartilage volumes with regard to medial posterior weight-bearing, medial posterior, lateral posterior weight-bearing, and lateral posterior portions (p = 0.006, 0.023, 0.017 and 0.002, respectively). These results were consistent with the previous studies showing that knee cartilage loss occurs frequently in these portions due to an anterior subluxation of the tibia accompanied by ACL tear. With our method, knee cartilage volumes could be measured quantitatively with conventional MR imaging in patients who underwent ACL reconstruction surgery.
• Preoperative knee deformity and kinematics impact postoperative knee kinematics in total knee arthroplasty

  Naoki Seito, Tomohiro Onodera, Yasuhiko Kasahara, Eiji Kondo, Norimasa Iwasaki, Tokifumi Majima

  The Knee 24 6 1462 - 1468 2017年12月 [査読有り][通常論文]
  
• Depletion of Gangliosides Enhances Articular Cartilage Repair in Mice

  Masatake Matsuoka, Tomohiro Onodera, Kentaro Homan, Fumio Sasazawa, Jun-ichi Furukawa, Daisuke Momma, Rikiya Baba, Kazutoshi Hontani, Zenta Joutoku, Shinji Matsubara, Tadashi Yamashita, Norimasa Iwasaki

  SCIENTIFIC REPORTS 7 43729  2017年03月 [査読有り][通常論文]
   

  Elucidation of the healing mechanisms in damaged tissues is a critical step for establishing breakthroughs in tissue engineering. Articular cartilage is clinically one of the most successful tissues to be repaired with regenerative medicine because of its homogeneous extracellular matrix and few cell types. However, we only poorly understand cartilage repair mechanisms, and hence, regenerated cartilage remains inferior to the native tissues. Here, we show that glycosylation is an important process for hypertrophic differentiation during articular cartilage repair. GM3, which is a precursor molecule for most gangliosides, was transiently expressed in surrounding damaged tissue, and depletion of GM3 synthase enhanced cartilage repair. Gangliosides also regulated chondrocyte hypertrophy via the Indian hedgehog pathway. These results identify a novel mechanism of cartilage healing through chondrocyte hypertrophy that is regulated by glycosylation. Manipulation of gangliosides and their synthases may have beneficial effects on articular cartilage repair.
• A rare case of multiple phosphaturic mesenchymal tumors along a tendon sheath inducing osteomalacia

  Ryuta Arai, Tomohiro Onodera, Mohamad Alaa Terkawi, Tomoko Mitsuhashi, Eiji Kondo, Norimasa Iwasaki

  BMC MUSCULOSKELETAL DISORDERS 18 1 79  2017年02月 [査読有り][通常論文]
   

  Background: Tumor-induced osteomalacia (TIO) is a rare paraneoplastic syndrome characterized by renal phosphate wasting, hypophosphatemia, reduction of 1,25-dihydroxyl vitamin D, and bone calcification disorders. Tumors associated with TIO are typically phosphaturic mesenchymal tumors that are bone and soft tissue origin and often present as a solitary tumor. The high production of fibroblast growth factor 23 (FGF23) by the tumor is believed to be the causative factor responsible for the impaired renal tubular phosphate reabsorption, hypophosphatemia and osteomalacia. Complete removal of the tumors by surgery is the most effective procedure for treatment. Identification of the tumors by advanced imaging techniques is difficult because TIO is small and exist within bone and soft tissue. However, systemic venous sampling has been frequently reported to be useful for diagnosing TIO patients. Case presentation: We experienced a case of 39-year-old male with diffuse bone pain and multiple fragility fractures caused by multiple FGF23-secreting tumors found in the hallux. Laboratory testing showed hypophosphatemia due to renal phosphate wasting and high levels of serum FGF23. Contrast-enhanced MRI showed three soft tissue tumors and an intraosseous tumor located in the right hallux. Systemic venous sampling of FGF23 revealed an elevation in the right common iliac vein and external iliac vein, which suggested that the tumors in the right hallux were responsible for overproduction of FGF23. Thereafter, these tumors were surgically removed and subjected to histopathological examinations. The three soft tissue tumors were diagnosed as phosphaturic mesenchymal tumors, which are known to be responsible for TIO. The fourth tumor had no tumor structure and was consisting of hyaline cartilage and bone tissue. Immediately after surgery, we noted a sharply decrease in serum level of FGF23, associated with an improved hypophosphatemia and a gradual relief of systematic pain that disappeared within two months of surgery. Conclusion: The authors reported an unusual case of osteomalacia induced by multiple phosphaturic mesenchymal tumors located in the hallux. Definition of tumors localization by systemic venous sampling led to successful treatment and cure this patient. The presence of osteochondral tissues in the intraosseous tumor might be developed from undifferentiated mesenchymal cells due to high level of FGF23 produced by phosphaturic mesenchymal tumors.
• Comparison of femoral head translation following eccentric rotational acetabular osteotomy and rotational acetabular osteotomy

  Tohru Irie, Daisuke Takahashi, Tsuyoshi Asano, Ryuta Arai, Takuya Konno, Tomohiro Onodera, Eiji Kondo, Norimasa Iwasaki

  HIP INTERNATIONAL 27 1 49 - 54 2017年01月 [査読有り][通常論文]
   

  Purpose: Eccentric rotational acetabular osteotomy (ERAO) is a modification of rotational acetabular osteotomy (RAO); it has been reported that ERAO allows the femoral head to translate medially and distally. However, no study has compared femoral head translation following RAO or ERAO. The purpose of this study was to compare immediate postoperative translation of the femoral head after RAO and ERAO in comparison with the preoperative position by radiological methods. Methods: Patients treated by RAO or ERAO between 2006 and 2014 were retrospectively evaluated. 19 hips (17 patients) were treated with RAO, and 25 hips (22 patients) were treated with ERAO. The acetabular roof angle and the location of the femoral head were measured on anteroposterior pelvic radiographs. Results: The mean preoperative acetabular roof angle was 20.9 degrees in the RAO group and 22.0 degrees in the ERAO group, showing no significant difference. The mean acetabular roof angle immediately postoperatively was -0.5 degrees in the RAO group and -0.4 degrees in the ERAO group, again showing no significant difference. The mean femoral head translation immediately postoperatively was 3.1 mm (95% confidence interval (CI), 1.5-4.7 mm) laterally and 3.0 mm (95% CI, 1.3-4.7 mm) proximally in the RAO group and 0.8 mm (95% CI, -0.7-2.3 mm) medially and 2.8 mm (95% CI, 1.5-4.1 mm) distally in the ERAO group; this difference was very highly significant (p<0.001). Conclusions: In contrast with RAO, ERAO resulted in significant femoral head translation both medially and distally immediately postoperatively.
• Efficacy of teriparatide in the treatment of nontraumatic osteonecrosis of the femoral head: a retrospective comparative study with alendronate

  Ryuta Arai, Daisuke Takahashi, Masahiro Inoue, Tohru Irie, Tsuyoshi Asano, Takuya Konno, Mohamad Alaa Terkawi, Tomohiro Onodera, Eiji Kondo, Norimasa Iwasaki

  BMC MUSCULOSKELETAL DISORDERS 18 1 24  2017年01月 [査読有り][通常論文]
   

  Background: Collapse of the femoral head associated with nontraumatic osteonecrosis (NOFH) is one of the most common causes of disability in young adult patients. Excessive bone resorption by osteoclast coincident with the suppression of osteogenesis are believed to be responsible for collapse progression. Alendronate that inhibits bone resorption by inducing osteoclast apoptosis has been traditionally used for treating NOFH; however, several reports documented serious complications by the use of this drug. On the other hand, teriparatide activates osteoblasts leading to an overall increase in bone volume, and is expected to reduce the progression of femoral head collapse in NOFH. Therefore, the present study was undertaken to examine pharmacological effects of teriparatide on collapse progression of NOFH and to compare these effects with alendronate. Methods: We conducted a retrospective study in our facility for comparing the pharmacological effects of teriparatide and alendronate on 32 NOFH patients diagnosed with osteoporosis. Between 2007 and 2013, patients were treated with daily administration of 20 mu g teriparatide (15 patients: 18 hips), or with 35 mg of alendronate once a week (17 patients: 22 hips). The mean period of follow-up was 18.7 months. The progression of collapse was evaluated prior to the administration and later every three months by anteroposterior radiographs. Collapse progression with > 1 mm was defined as advanced collapse, while with < 1 mm was defined as stable radiologic disease. Student's t-test and the chi-square test was used to do compare the pharmacological effects of the two groups. Results: Treatment with terparatide had a tendency to reduce the rate of advanced collapse as compared to that with alendronate (p = 0.105). Kaplan-Meier curves related to stable radiologic disease showed that teriparatide-treated patients had better stable states than these treated with alendronate (p = 0.08, log-rank test). Moreover, treatment with teriparatide resulted in a significant reduction in collapse progression as compared to that with alendronate, noted at the end of follow-up period (p = 0.049). Conclusion: The present study suggests that teriparatide has greater pharmacological effects than alendronate for treating NOFH and preventing the collapse of femoral head.
• マテリアルと細胞 ultra-purified alginate gel(UPAL gel)を用いたマウスiPS細胞からの軟骨分化誘導

  本谷 和俊, 小野寺 智洋, 寺島 理代, 門間 太輔, 松岡 正剛, 馬場 力哉, 上徳 善太, 松原 新史, 宝満 健太郎, 菱村 亮介, 金 佑泳, 徐 亮, 岩崎 倫政

  日本バイオマテリアル学会大会予稿集 シンポジウム2016 92 - 92 日本バイオマテリアル学会 2016年11月
• Use of a pneumatic tourniquet in total knee arthroplasty increases the risk of distal deep vein thrombosis: A prospective, randomized study

  Noriaki Mori, Shoichi Kimura, Tomohiro Onodera, Norimasa Iwasaki, Izumi Nakagawa, Takeshi Masuda

  KNEE 23 5 887 - 889 2016年10月 [査読有り][通常論文]
   

  Background: There has been controversy regarding the incidence of deep vein thrombosis (DVT) after total knee arthroplasty (TKA) with or without the use of a tourniquet. The aim of this randomized, prospective study was to clarify the effects of tourniquet use on DVT in TKA. Methods: The subjects were 109 patients scheduled to undergo TKA from April 2008 to March 2009 before the establishment of the American Association of Orthopedic Surgeons (AAOS) practice guidelines. They were randomized into two groups: 51 patients in the tourniquet group (group T) and 52 patients in the control group without a tourniquet (group C). We investigated the thrombotic presence using ultrasonography one week after surgery and compared both groups. Results: There was no difference in the rate of proximal DVTs (P = 0.63). However, the risk of distal DVT was significantly higher in group T than in group C (52.9% vs. 23.1%; P = 0.002). Conclusions: Use of the tourniquet in TKA increased the risk of distal DVT. (C) 2016 Elsevier B.V. All rights reserved.
• A Novel Bone Marrow Stimulation Technique Augmented by Administration of Ultrapurified Alginate Gel Enhances Osteochondral Repair in a Rabbit Model

  Rikiya Baba, Tomohiro Onodera, Daisuke Momma, Masatake Matsuoka, Kazutoshi Hontani, Sameh Elmorsy, Kaori Endo, Masahiro Todoh, Shigeru Tadano, Norimasa Iwasaki

  TISSUE ENGINEERING PART C-METHODS 21 12 1263 - 1273 2015年12月 [査読有り][通常論文]
   

  Cartilage injuries are a common health problem resulting in the loss of daily activities. Bone marrow stimulation technique, one of the surgical techniques for the cartilage injuries, is characterized by technical simplicity and less invasiveness. However, it has been shown to result in fibrous or fibrocartilaginous repair with inferior long-term results. This study focused on using ultrapurified alginate gel (UPAL gel) as an adjuvant scaffold in combination with a bone marrow stimulation technique. The objective of this study was to assess the efficacy of a bone marrow stimulation technique augmented by UPAL gel in a rabbit osteochondral defect model. To achieve this goal, three experimental groups were prepared as follows: defects without intervention, defects treated with a bone marrow stimulation technique, and defects treated with a bone marrow stimulation technique augmented by UPAL gel. The macroscopic and histological findings of the defects augmented by UPAL gel improved significantly more than those of the others at 16 weeks postoperatively. The combination technique elicited hyaline-like cartilage repair, unlike bone marrow stimulation technique alone. This combination procedure has the potential of improving clinical outcomes after use of a bone marrow stimulation technique for articular cartilage injuries.
• External rotation of the femoral component decreases patellofemoral contact stress in total knee arthroplasty

  Takashi Terashima, Tomohiro Onodera, Naohiro Sawaguchi, Yasuhiko Kasahara, Tokifumi Majima

  KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY 23 11 3266 - 3272 2015年11月 [査読有り][通常論文]
   

  The purpose of this study was to investigate the relationship between patellofemoral (PF) contact stress in vivo and the alignment of the femoral component in patients after total knee arthroplasty (TKA). Thirty knees with medial compartment osteoarthritis that underwent mobile-bearing TKA with modified gap technique were evaluated. Surgery was performed using a subvastus approach to eliminate the effect of the approach to muscle balance, with a computed tomography-based navigation system (Vector Vision 1.61; Brain Lab, Heimstetten, Germany). PF contact stress was measured by a Flexiforce pressure sensor (Nitta Co., Ltd., Osaka, Japan) intraoperatively, and the results were compared with the alignment of the femoral component after TKA. The PF contact stress was not correlated with sagittal and coronal alignment of the femoral component and patellar tracking, whereas rotational alignment of the femoral component was negatively correlated with PF contact stress (r = -0.718, p < 0.01). Regarding the alignment of the femoral component, only the rotational alignment of the femoral component was correlated with PF contact stress. PF contact stress decreased more as the femoral component rotated more externally. Case control study, Level III.
• Therapeutic effects of intra-articular ultra-purified low endotoxin alginate administration on an experimental canine osteoarthritis model

  Yukinori Tsukuda, Tomohiro Onodera, Masayuki Ito, Yasuharu Izumisawa, Yasuhiko Kasahara, Tatsuya Igarashi, Nobuo Ohzawa, Masahiro Todoh, Shigeru Tadano, Norimasa Iwasaki

  JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 103 11 3441 - 3448 2015年11月 [査読有り][通常論文]
   

  Objective: This study aimed to elucidate the therapeutic effects of intra-articular administration of ultra-purified low endotoxin alginate (UPLE-alginate) on osteoarthritis (OA) using a canine anterior cruciate ligament transection (ACLT) model. Design: We used 20 beagle dogs. ACLT was performed on the left knee of each dog and a sham operation was performed on the right knee as a control. All animals were randomly divided into the control (saline) and therapeutic (UPLE-alginate) groups. Animals in the control and therapeutic groups received weekly injections with 0.7 mL normal saline or 0.7 mL 0.5% UPLE-alginate, respectively, from 0 to 3 weeks after ACLT or sham operation. At 9 weeks after ACLT, the knee joints of all animals were observed using arthroscopy. All animals were euthanized at 14 weeks after ACLT and evaluated using morphologic assessment, histologic assessment, and biomechanical testing. Results: Arthroscopic findings showed intact cartilage surface in both groups. Morphologic findings in the therapeutic group showed milder degeneration compared with those of the control group, but there were no significant differences between groups. Histologic scores of the medial femoral condyle (MFC) and lateral femoral condyle (LFC) were better in the therapeutic group than the control group (MFC: p=0.009, LFC: p=0.009). Joint lubrication did not differ significantly between groups. Conclusion: Intra-articular administration of UPLE-alginate in the early stage of OA slowed disease progression in canines. UPLE-alginate may have potential as a therapeutic agent for OA patients and reduce the number of patients who need to undergo total joint arthroplasty. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3441-3448, 2015.
• 変形性足関節症、リウマチ性足関節炎、距骨骨壊死におけるIlizarov創外固定器を用いた足関節固定術の臨床成績の検討

  深谷 英昭, 小野寺 智洋, 中野 宏昭, 新井 隆太, 高橋 大介, 岩崎 倫政

  日本足の外科学会雑誌 36 1 157 - 161 日本足の外科学会 2015年09月01日 [査読無し][通常論文]
   

  【背景】足関節固定術に至る疾患として変形性足関節症(以下OA)・リウマチ性足関節炎(以下RA)・距骨骨壊死(以下AVN)などが挙げられ、疾患により骨癒合率や臨床成績が異なる。本研究の目的は、Ilizarov創外固定器を用いた足関節固定術(以下Ilizarov足関節固定術)において疾患毎の臨床成績を比較検討することである。【方法】対象は1999年4月〜2014年3月に当科にてIlizarov足関節固定術を施行した38例。内訳はOA群:21例、RA群:12例、AVN群:5例であった。骨癒合率・臨床成績(AOFAS scale)・創外固定器抜去までの期間・合併症を各群で比較検討した。【結果】全症例で骨癒合が得られた。創外固定器抜去までの平均期間はOA群:96.7日、RA群:102.7日、AVN群:102.6日で有意差を認めなかった。術後平均AOFAS scaleはOA群:80.8、RA群81.6、AVN群:71.7で有意差を認めなかった。【結論】Ilizarov足関節固定術は疾患に関わらず安定した臨床成績を示し、骨癒合が困難と予想される疾患に対して有用であると考えられた。(著者抄録)
• 足趾腫瘍による腫瘍性骨軟化症の一例

  新井 隆太, 小野寺 智洋, 松原 新史, 山本 励志, 岩崎 倫政

  日本足の外科学会雑誌 36 1 340 - 344 日本足の外科学会 2015年09月01日 [査読無し][通常論文]
   

  腫瘍性骨軟化症(tumor-induced osteomalacia;TIO)は、fibroblast growth factor 23(FGF23)を過剰産生する腫瘍によって惹起される腫瘍随伴症候群である。今回、足趾に発生した腫瘍によるTIOを経験した。症例は39歳男性。誘因なく全身痛が出現。腎性にリン排泄が亢進した低リン血症を認め、血中FGF23濃度は基準値上限の約4倍であった。TIOが疑われ、病巣検索目的に実施した全身静脈サンプリングでは右総腸骨静脈と右外腸骨静脈でFGF23濃度の上昇を認めた。MRIでは右第1足趾に3つの軟部腫瘤と1つの末節骨骨内腫瘤が認められた。4つの腫瘤を摘出した。病理組織診断では3つの軟部腫瘤でphosphaturic mesenchymal tumorであったが、骨内腫瘤には骨組織と硝子軟骨様組織、線維組織が認められたのみであった。術後、血中FGF23濃度の速やかな低下とリン濃度の回復、症状の改善が認められた。TIOは腫瘍切除により完治が可能であるとされ本症例も同様であった。また、FGF23の全身静脈サンプリングは腫瘍局在同定の一助になり有用であった。(著者抄録)
• Syndecan 4 Regulation of the Development of Autoimmune Arthritis in Mice by Modulating B Cell Migration and Germinal Center Formation.

  Tsutomu Endo, Koyu Ito, Junko Morimoto, Masashi Kanayama, Daichi Ota, Masahiro Ikesue, Shigeyuki Kon, Daisuke Takahashi, Tomohiro Onodera, Norimasa Iwasaki, Toshimitsu Uede

  Arthritis & rheumatology (Hoboken, N.J.) 67 9 2512 - 22 2015年09月 [査読有り][通常論文]
   

  OBJECTIVE: Syndecan 4 has been implicated as a critical mediator of inflammatory responses because of its functions as a coreceptor and reservoir for growth factors and chemokines. Although syndecan 4 is known to be expressed on B cells, its role in immune responses remains unclear. The purpose of this study was to investigate the contribution of syndecan 4 to the development of immune arthritis in murine models. METHODS: The clinical severity of 3 immunopathologically distinct models, collagen-induced arthritis (CIA), antigen-induced arthritis (AIA), and collagen antibody-induced arthritis (CAIA), was evaluated in wild-type (WT) mice and in syndecan 4-deficient (Sdc4(-/-) ) mice. Germinal center (GC) formation, B cell profiles, humoral immune responses, and B cell migration were analyzed during the course of CIA. RESULTS: Sdc4(-/-) mice were resistant to the induction of CIA, which is T cell and B cell dependent, but AIA and CAIA, which are T cell dependent and T cell independent, respectively, occurred with equal frequency in WT mice and Sdc4(-/-) mice. Furthermore, Sdc4(-/-) mice had reduced numbers of B cells and deficient GC formation in draining lymph nodes (DLNs) during the course of CIA, resulting in reduced production of collagen-specific autoantibodies. Compared with B cells from WT mice, B cells from Sdc4(-/-) mice showed reduced chemotactic migration toward stromal cell-derived factor 1 (SDF-1) and reduced SDF-1-mediated Akt phosphorylation. Consistent with these findings, in vivo transfer experiments showed that fewer Sdc4(-/-) B cells than WT B cells were found in and around the follicles in the DLNs. CONCLUSION: Our findings suggest that syndecan 4 contributes to the development of CIA by promoting GC formation and autoantibody production through its regulation of SDF-1-mediated B cell migration.
• An Articular Cartilage Repair Model in Common C57Bl/6 Mice

  Masatake Matsuoka, Tomohiro Onodera, Fumio Sasazawa, Daisuke Momma, Rikiya Baba, Kazutoshi Hontani, Norimasa Iwasaki

  TISSUE ENGINEERING PART C-METHODS 21 8 767 - 772 2015年08月 [査読有り][通常論文]
   

  To analyze the genetic and biomolecular mechanisms underlying cartilage repair, an optimized mouse model of osteochondral repair is required. Although several models of articular cartilage injury in mice have recently been established, the articular surface in adult C57Bl/6 mice heals poorly. Since C57Bl/6 mice are the most popular strain of genetically manipulated mice, an articular cartilage repair model using C57Bl/6 mice would be helpful for analysis of the mechanisms of cartilage repair. The purpose of this study was to establish a cartilage repair model in C57Bl/6 mice using immature animals. To achieve this goal, full-thickness injuries were generated in 3-week-old (young), 4-week-old (juvenile), and 8-week-old (adult) C57Bl/6 mice. To investigate the reproducibility and consistency of full-thickness injuries, mice were sacrificed immediately after operation, and cartilage thickness at the patellar groove, depth of the cartilage injury, cross-sectional width, and cross-sectional area were compared among the three age groups. The depth of cartilage injury/cartilage thickness ratio (%depth) and the coefficient of variation (CV) for each parameter were also calculated. At 8 weeks postoperatively, articular cartilage repair was assessed using a histological scoring system. With respect to the reproducibility and consistency of full-thickness injuries, cartilage thickness, depth of cartilage injury, and cross-sectional area were significantly larger in young and juvenile mice than in adult mice, whereas cross-sectional width and %depth were almost equal among the three age groups. CVs of %depths were less than 10% in all groups. With respect to articular cartilage repair, young and juvenile mice showed superior results. In conclusion, we established a novel cartilage repair model in C57Bl/6 mice. This model will be valuable in achieving mechanistic insights into the healing process of the joint surface, as it will facilitate the use of genetically modified mice, which are most commonly developed on a C57Bl/6 background.
• A Comparative Study With In Vitro Ultrasonographic and Histologic Grading of Metatarsal Head Cartilage in Rheumatoid Arthritis

  Tomohiro Onodera, Yasuhiko Kasahara, Toshiyuki Kasemura, Yuki Suzuki, Eiji Kondo, Norimasa Iwasaki

  FOOT & ANKLE INTERNATIONAL 36 7 774 - 779 2015年07月 [査読有り][通常論文]
   

  Background: Ultrasonography is among the valid methods to assess articular cartilage in the foot. This study aimed to evaluate the validity of ultrasonographic grading to assess metatarsal head articular cartilage for rheumatoid forefoot deformity in vivo and to compare the findings with in vitro ultrasonographic and histologic gradings. Methods: Participants were 15 patients scheduled to undergo resection arthroplasty of the metatarsal heads of the lesser toes because of rheumatoid arthritis of the metatarsophalangeal joints. Ultrasonographic examination was performed in vivo the day before surgery. Specimens of the second to fifth metatarsal heads taken intraoperatively were graded from in vitro ultrasonographic and histologic evaluations. Correlations among in vivo ultrasonographic, in vitro ultrasonographic, and histologic gradings were analyzed. Results: In 46 metatarsal heads, the distribution of grading ranged from grade 1 to 6 for in vivo ultrasonographic examinations and from grade 1 to 4 for histologic examinations. In vivo ultrasonographic grading showed significant correlation to both in vitro ultrasonographic grading (P < .001, R = 0.74) and histologic grading (P < .001, R = 0.67). Conclusions: The significant correlations between in vivo ultrasonographic and histologic gradings suggest that a semiquantitative in vivo ultrasonographic assessment of forefoot deformity in rheumatoid arthritis may be possible. Ultrasonographic grading may prove useful for pre- and postoperative evaluation of remaining joint function in rheumatoid forefoot deformity. An ultrasonographic grading system for remaining joint surfaces might be helpful in selecting surgical procedures such as joint-sparing osteotomy and metatarsal head resection. Level of Evidence: Level IV, case series.
• Correlation Between Knee Kinematics and Patellofemoral Contact Pressure in Total Knee Arthroplasty

  Takuya Konno, Tomohiro Onodera, Yusuke Nishio, Yasuhiko Kasahara, Norimasa Iwasaki, Tokifumi Majima

  JOURNAL OF ARTHROPLASTY 29 12 2305 - 2308 2014年12月 [査読有り][通常論文]
   

  The aim of this study is to evaluate the relationship between patellofemoral contact stress and intraoperative knee kinematic patterns after mobile bearing total knee arthroplasty (TKA). Medial osteoarthritic knees of forty-six posterior-stabilized total knee prostheses were evaluated using a computed tomography-guided navigation system. Subjects were divided into two groups based on intraoperative knee kinematic patterns: the medial pivot group (n = 19) and the non-medial pivot group (n = 27). Mean intraoperative patellofemoral contact stress was significantly lower in the medial pivot group than in the non-medial pivot group (1.7 MPa vs. 3.2 MPa, P < 0.05). An intraoperative medial pivot pattern results in reduced patello-femoral contact stress. (C) 2014 Elsevier Inc. All rights reserved.
• Quantitative glycomics monitoring of induced pluripotent- and embryonic stem cells during neuronal differentiation

  Michiyo Terashima, Maho Amano, Tomohiro Onodera, Shin-Ichiro Nishimura, Norimasa Iwasaki

  STEM CELL RESEARCH 13 3 454 - 464 2014年11月 [査読有り][通常論文]
   

  Alterations in the structure of cell surface glycoforms occurring during the stages of stem cell differentiation remain unclear. We describe a rapid glycoblotting-based cellular glycomics method for quantitatively evaluating changes in glycoform expression and structure during neuronal differentiation of murine induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs). Our results show that changes in the expression of cellular N-glycans are comparable during the differentiation of iPSCs and ESCs. The expression of bisect-type N-glycans was significantly up-regulated in neurons that differentiated from both iPSCs and ESCs. From a glycobiological standpoint, iPSCs are an alternative neural cell source in addition to ESCs. (C) 2014 The Authors. Published by Elsevier B.V.
• Intraoperative Medial Pivot Affects Deep Knee Flexion Angle and Patient-Reported Outcomes After Total Knee Arthroplasty

  Yusuke Nishio, Tomohiro Onodera, Yasuhiko Kasahara, Daisuke Takahashi, Norimasa Iwasaki, Tokifumi Majima

  JOURNAL OF ARTHROPLASTY 29 4 702 - 706 2014年04月 [査読有り][通常論文]
   

  The aim of this study was to evaluate the relationship between clinical results including patient-reported outcomes and intraoperative knee kinematic patterns after total knee arthroplasty (TKA). A cross-sectional survey of forty consecutive medial osteoarthritis patients who had a primary TKA using a CT-based navigation system was conducted. Subjects were divided into two groups based on intraoperative kinematic patterns: a medial pivot group (n = 20) and a non-medial pivot group (n = 20). Subjective outcomes with the new Knee Society Score and clinical outcomes were evaluated. The functional activities, patient satisfaction and the knee flexion angle of the medial pivot group were significantly better than those of the non-medial pivot group. An intraoperative medial pivot pattern positively influences deep knee flexion and patient-reported outcomes. (C) 2014 Elsevier Inc. All rights reserved.
• 股関節後方脱臼骨折の治療成績

  清水 智弘, 高橋 大介, 笠原 靖彦, 小野寺 智洋, 西尾 悠介, 紺野 拓也, 岩崎 倫政, 眞島 任史, 井上 正弘, 田辺 明彦, 入江 徹

  北海道整形災害外科学会雑誌 55 2 346 - 346 北海道整形災害外科学会 2014年03月
• Depletion of gangliosides enhances cartilage degradation in mice

  F. Sasazawa, T. Onodera, T. Yamashita, N. Seito, Y. Tsukuda, N. Fujitani, Y. Shinohara, N. Iwasaki

  OSTEOARTHRITIS AND CARTILAGE 22 2 313 - 322 2014年02月 [査読有り][通常論文]
   

  Objective: Glycosphingolipids (GSLs) are ubiquitous membrane components that play a functional role in maintaining chondrocyte homeostasis. We investigated the potential role of gangliosides, one of the major components of GSLs, in osteoarthritis (OA) pathogenesis. Design: Both age-associated and instability-induced OA models were generated using GM3 synthase knockout (GM3.5(-/-)) mice. A cartilage degradation model and transiently GM3S-transfected chondrocytes were analyzed to evaluate the function of gangliosides in OA development. The amount of each series of GSLs in chondrocytes after IL-1 alpha stimulation was profiled using mass spectrometry (MS). Results: OA changes in GM3.5(-/-) mice were dramatically enhanced with aging compared to those in wildtype (WT) mice. GM3.5(-/-) mice showed more severe instability-induced pathologic OA in vivo. Ganglioside deficiency also led to the induction of matrix metalloproteinase (MMP)-13 and ADAMTS-5 secretion and chondrocyte apoptosis in vitro. In contrast, transientGM3.5(-/-) transfection of chondrocytes suppressed MMP-13 and ADAMTS-5 expression after interleukin (IL)-1 alpha stimulation. GSL profiling revealed the presence of abundant gangliosides in chondrocytes after IL-1 alpha stimulation. Conclusion: Gangliosides play a critical role in OA pathogenesis by regulating the expression of MMP-13 and ADAMTS-5 and chondrocyte apoptosis. Based on the obtained results, we propose that gangliosides are potential target molecules for the development of novel OA treatments. (C) 2013 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
• Celecoxib does not affect the release of hyaluronic acid in end stage osteoarthritic joints

  Daisuke Takahashi, Tokifumi Majima, Tomohiro Onodera, Yasuhiko Kasahara, Masahiro Inoue, Toru Irie, Toshiyuki Kasemura

  MODERN RHEUMATOLOGY 23 5 934 - 938 2013年09月 [査読有り][通常論文]
   

  Non-steroidal anti-inflammatory drugs play a major role in the management of osteoarthritis (OA). However, it remains unknown whether these drugs affect cartilage and synovial metabolism in osteoarthritic joints. The aim of this study was to evaluate the effects of a selective cyclooxygenase (COX)-2 inhibitor, celecoxib, on synovial fluids and tissues in severely osteoarthritic knees. Patients were randomized into three groups and medicated two weeks prior to total knee arthroplasty with celecoxib, diclofenac sodium, or no medication (control). We checked for the presence of matrix metalloproteinase-3 (MMP-3), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and hyaluronic acid (HA) in the synovial fluids of all three groups. MMP-3 significantly decreased in the celecoxib-treated patients (p = 0.0031). On the other hand, there were no significant differences among the three groups in their TNF-alpha and IL-1 beta levels. HA in the joint fluid was significantly increased in the diclofenac-treated group, while HA was not changed in the celecoxib-treated patients. Our study suggests that celecoxib did not affect the level of HA in the joint fluid in the knee in severe OA, in contrast to the effect of the dual COX inhibitor.
• Posterior Femoral Condylar Offset After Total Knee Replacement in The Risk of Knee Flexion Contracture

  Tomohiro Onodera, Tokifumi Majima, Osamu Nishiike, Yasuhiko Kasahara, Daisuke Takahashi

  JOURNAL OF ARTHROPLASTY 28 7 1112 - 1116 2013年08月 [査読有り][通常論文]
   

  The aim of this study was to clarify the risk of knee flexion contracture associated with a posterior femoral condylar offset after total knee replacement (TKR). Radiographs from 100 healthy Japanese volunteers were included in the study. We evaluated femoral component posterior offset in various implants and compared them with the normal Japanese knee. Posterior offset of the femoral condyle is up to a maximum of 4.7 times greater than that of the healthy Japanese knee in all knee implants. Excess posterior offset of the femoral condyle in TKR prostheses may cause knee joint flexion contracture due to the relative shortening of the posterior soft tissue. (c) 2013 Elsevier Inc. All rights reserved.
• Sagittal Alignment of the First Metatarsophalangeal Joint after Arthrodesis for Rheumatoid Forefoot Deformity

  Akihiko Tanabe, Tokifumi Majima, Tomohiro Onodera, Naohiro Sawaguchi, Takuya Watanabe, Yasuhiko Kasahara, Daisuke Takahashi

  JOURNAL OF FOOT & ANKLE SURGERY 52 3 343 - 347 2013年05月 [査読有り][通常論文]
   

  The present study assessed the midterm results of reconstruction for rheumatoid forefoot deformity with arthrodesis of the first metatarsophalangeal (MTP) joint, scarf osteotomy, resection arthroplasty of the metatarsal head of the lesser toes, and surgical repair of hammertoe deformity (arthrodesis of the proximal interphalangeal joint). Special focus was placed on the sagittal alignment of the first metatarsophalangeal joint after arthrodesis. We retrospectively evaluated the postoperative clinical outcomes and radiographic findings for 16 consecutive female patients (20 feet) with symptomatic rheumatoid forefoot deformities. The mean duration of follow-up was 7.9 (range 4 to 13) years. All first MTP joints and first metatarsal bones were fused successfully. The mean value of the American Orthopaedic Foot and Ankle Society and Japanese Society for Foot Surgery clinical scores significantly improved overall, except for 2 patients (10%), who complained of first toe pain at the final follow-up visit owing to sagittal misalignment of the fused first MTP joint. Sagittal alignment of the first metatarsal varies greatly because of the rheumatoid midfoot and hindfoot deformities. Therefore, inclination of the first metatarsal should be considered when determining the first MTP joint sagittal fusion angle. (C) 2013 by the American College of Foot and Ankle Surgeons. All rights reserved.
• Hemorrhagic-acquired factor XIII deficiency associated with tocilizumab for treatment of rheumatoid arthritis

  Masatake Matsuoka, Tokifumi Majima, Tomohiro Onodera, Masahiro Ieko, Masayoshi Souri, Akitada Ichinose, Takashi Kurita, Yasuhiko Kasahara, Masahiro Inoue, Daisuke Takahashi

  INTERNATIONAL JOURNAL OF HEMATOLOGY 96 6 781 - 785 2012年12月 [査読有り][通常論文]
   

  Factor XIII (FXIII) is the final enzyme in the coagulation cascade. Acquired FXIII deficiency is caused by inhibitors of FXIII or decreased synthesis and/or increased consumption of FXIII, which leads to severe bleeding. Recently, we experienced a case of hemorrhagic-acquired factor XIII deficiency that occurred during treatment with the IL-6 inhibitor tocilizumab for rheumatoid arthritis. A 48-year-old man was referred because of right hip pain due to a hematoma. Laboratory findings showed that routine coagulation tests were normal, while FXIII activity was slightly low (52.4 %). The patient was successfully treated with plasma-derived factor XIII concentrates. The time course of recovery suggests that tocilizumab might have inhibited FXIII production. To our knowledge, this is the first report of acquired factor XIII deficiency associated with administering of tocilizumab. When recurrent bleeding is seen during administering of tocilizumab, acquired factor XIII deficiency may have been induced, thus attending physicians should consider this disease in a differential diagnosis.
• Outcome of Transfibular Ankle Arthrodesis with Ilizarov Apparatus

  Tomohiro Onodera, Tokifumi Majima, Yasuhiko Kasahara, Daisuke Takahashi, Shuji Yamazaki, Ryo Ando, Akio Minami

  FOOT & ANKLE INTERNATIONAL 33 11 964 - 968 2012年11月 [査読有り][通常論文]
   

  Background: Both transfibular ankle arthrodesis with fibular onlay grafting and ankle arthrodesis using the Ilizarov apparatus have resulted in acceptable fusion rates. This study analyzed outcomes in the authors' practice for all patients who underwent transfibular ankle arthrodesis with fibular onlay grafting using the Ilizarov apparatus. Materials and Methods: A retrospective review was conducted of all patients who experienced severe ankle pain associated with osteoarthritis, rheumatoid arthritis, talar osteonecrosis, or septic arthritis and underwent transfibular ankle arthrodesis with an Ilizarov apparatus between 1995 and 2010. Postoperative clinical outcomes were evaluated for 42 consecutive patients (43 feet) who received primary or revision ankle arthrodesis using the Ilizarov technique at two centers. Mean duration of follow-up was 4.1 (range, 1.5 to 13) years. Outcomes were assessed using the ankle-hindfoot scale of the American Orthopaedic Foot and Ankle Society. Results: All ankles fused successfully. Mean clinical outcome improved significantly from 36.2 (range, 8 to 64) preoperatively to 77.4 (range, 0 to 86; p < .05) postoperatively. Conclusion: Transfibular ankle arthrodesis using the Ilizarov external fixation system and fibular onlay strut grafting can achieve a very high rate of bony union and lead to general improvements in clinical outcome. Forefoot stabilization with a forefoot ring achieved rigid stabilization resulting in a shorter duration of external fixation.
• Long-term stress distribution patterns of the ankle joint in varus knee alignment assessed by computed tomography osteoabsorptiometry

  Tomohiro Onodera, Tokifumi Majima, Norimasa Iwasaki, Tamotsu Kamishima, Yasuhiko Kasahara, Akio Minami

  INTERNATIONAL ORTHOPAEDICS 36 9 1871 - 1876 2012年09月 [査読有り][通常論文]
   

  The stress distribution of an ankle under various physiological conditions is important for long-term survival of total ankle arthroplasty. The aim of this study was to measure subchondral bone density across the distal tibial joint surface in patients with malalignment/instability of the lower limb. We evaluated subchondral bone density across the distal tibial joint in patients with malalignment/instability of the knee by computed tomography (CT) osteoabsorptiometry from ten ankles as controls and from 27 ankles with varus deformity/instability of the knee. The quantitative analysis focused on the location of the high-density area at the articular surface, to determine the resultant long-term stress on the ankle joint. The area of maximum density of subchondral bone was located in the medial part in all subjects. The pattern of maximum density in the anterolateral area showed stepwise increases with the development of varus deformity/instability of the knee. Our results should prove helpful for designing new prostheses and determining clinical indications for total ankle arthroplasty.
• Interruption of glycosphingolipid synthesis enhances osteoarthritis development in mice

  Naoki Seito, Tadashi Yamashita, Yukinori Tsukuda, Yuichiro Matsui, Atsushi Urita, Tomohiro Onodera, Takeomi Mizutani, Hisashi Haga, Naoki Fujitani, Yasuro Shinohara, Akio Minami, Norimasa Iwasaki

  ARTHRITIS AND RHEUMATISM 64 8 2579 - 2588 2012年08月 [査読有り][通常論文]
   

  Objective Glycosphingolipids (GSLs) are ubiquitous membrane components that modulate transmembrane signaling and mediate cell-to-cell and cell-to-matrix interactions. GSL expression is decreased in the articular cartilage of humans with osteoarthritis (OA). This study was undertaken to determine the functional role of GSLs in cartilage metabolism related to OA pathogenesis in mice. Methods We generated mice with knockout of the chondrocyte-specific Ugcg gene, which encodes an initial enzyme of major GSL synthesis, using the Cre/loxP system (Col2-Ugcg-/- mice). In vivo OA and in vitro cartilage degradation models were used to evaluate the effect of GSLs on the cartilage degradation process. Results Although Col2-Ugcg-/- mice developed and grew normally, OA changes in these mice were dramatically enhanced with aging, through the overexpression of matrix metalloproteinase 13 and chondrocyte apoptosis, compared to their wild-type (WT) littermates. Col2-Ugcg-/- mice showed more severe instability-induced pathologic OA in vivo and interleukin-1a (IL-1a)induced cartilage degradation in vitro. IL-1a stimulation of chondrocytes from WT mice significantly increased Ugcg messenger RNA expression and up-regulated GSL metabolism. Conclusion Our results indicate that GSL deficiency in mouse chondrocytes enhances the development of OA. However, this deficiency does not affect the development and organization of cartilage tissue in mice at a young age. These findings indicate that GSLs maintain cartilage molecular metabolism and prevent disease progression, although GSLs are not essential for chondrogenesis of progenitor and stem cells and cartilage development in young mice. GSL metabolism in the cartilage is a potential target for developing a novel treatment for OA.
• Ganglioside GM3 Has an Essential Role in the Pathogenesis and Progression of Rheumatoid Arthritis

  Yukinori Tsukuda, Norimasa Iwasaki, Naoki Seito, Masashi Kanayama, Naoki Fujitani, Yasuro Shinohara, Yasuhiko Kasahara, Tomohiro Onodera, Koji Suzuki, Tsuyoshi Asano, Akio Minami, Tadashi Yamashita

  PLOS ONE 7 6 e40136  2012年06月 [査読有り][通常論文]
   

  Rheumatoid arthritis (RA), a chronic systemic inflammatory disorder that principally attacks synovial joints, afflicts over 2 million people in the United States. Interleukin (IL)-17 is considered to be a master cytokine in chronic, destructive arthritis. Levels of the ganglioside GM3, one of the most primitive glycosphingolipids containing a sialic acid in the structure, are remarkably decreased in the synovium of patients with RA. Based on the increased cytokine secretions observed in in vitro experiments, GM3 might have an immunologic role. Here, to clarify the association between RA and GM3, we established a collagen-induced arthritis mouse model using the null mutation of the ganglioside GM3 synthase gene. GM3 deficiency exacerbated inflammatory arthritis in the mouse model of RA. In addition, disrupting GM3 induced T cell activation in vivo and promoted overproduction of the cytokines involved in RA. In contrast, the amount of the GM3 synthase gene transcript in the synovium was higher in patients with RA than in those with osteoarthritis. These findings indicate a crucial role for GM3 in the pathogenesis and progression of RA. Control of glycosphingolipids such as GM3 might therefore provide a novel therapeutic strategy for RA.
• Repair of Rabbit Osteochondral Defects by an Acellular Technique with an Ultrapurified Alginate Gel Containing Stromal Cell-Derived Factor-1

  Atsushi Sukegawa, Norimasa Iwasaki, Yasuhiko Kasahara, Tomohiro Onodera, Tatsuya Igarashi, Akio Minami

  TISSUE ENGINEERING PART A 18 9-10 934 - 945 2012年05月 [査読有り][通常論文]
   

  The objective of this study was to determine whether the local administration of stromal cell-derived factor-1 (SDF-1) using ultrapurified alginate gel (UPAL gel) could improve reparative tissues of osteochondral defects compared with those without treatment. For the investigation, a full-thickness osteochondral defect 4.5mm in diameter and 3mm in depth was created in the patella groove of the distal femur in rabbits. Local expression of SDF-1 protein was temporarily upregulated at 1 week after creating the osteochondral defect. The local administration of SDF-1 enhanced the migration of host cells, mainly bone marrow stromal cells (BMSCs), to the site of the osteochondral defect. In vitro cell migration assay supported this result. In the SDF-1 (UPAL gel containing SDF-1) treatment group, the histological scores and the compressive modulus of reparative tissues were significantly improved compared with the no-treatment and vehicle (UPAL gel without SDF-1) groups. On the other hand, SDF-1 did not influence the cellular proliferation and chondrogenesis of BMSCs. Based on the results obtained here, we speculate that SDF-1 enhances the reparative process of osteochondral injuries not through direct effects on the behavior of host cells, but through increased migration of host cells to the injured site. UPAL gel, as a vehicle material, may play an important role in chondrogenesis of recruited cells, mainly BMSCs. The cell-free approach with local administration of SDF-1 may be an effective strategy for developing a minimally invasive technique for cartilage tissue regeneration.
• Effect of the Japanese herbal medicine, Boiogito, on the osteoarthritis of the knee with joint effusion

  Tokifumi Majima, Masahiro Inoue, Yasuhiko Kasahara, Tomohiro Onodera, Daisuke Takahashi, Akio Minami

  Sports Medicine, Arthroscopy, Rehabilitation, Therapy and Technology 4 1 3  2012年01月10日 [査読有り][通常論文]
   

  Background: Boiogito (Japanese herbal medicine, Tsumura Co. Tokyo, Japan) contains sinomenin which inhibits inflammatory reactions. Since sinomenine is a principle component of the Boiogito, there is a possibility of it being effective on osteoarthritis (OA) of the knee with joint effusion. However, there is no report concerning the effectiveness of Boiogito on knee OA. The objective of the present study is to investigate the therapeutic effect of Boiogito on OA of the knee associated with joint effusion in a comparative study among randomly assigned groups.Methods: Study was performed using 50 patients who were diagnosed with primary osteoarthritis of the knee with joint effusion. The patients were randomly assigned to two groups: one group (25 patients) using both loxoprofen (2-{4-[(2-oxocyclopentyl) methyl]} propanoic acid) and Boiogito and the other group (25 patients) using loxoprofen, and were evaluated during a 12 week observation period. The assessment parameters including knee scores in the Knee Society Rating System including Knee score and Functional scores, amount of joint effusion by joint puncture in clinically detected cases, the 36-items short form of the Medical Outcome Study Questionnaire (SF-36) as a measurement of health related quality of life were used.Results: The knee scores based on the Knee Society Rating System were improved in both groups. The staircase climbing up and down ability in the Knee society rating system functional score was significantly improved in the group using Boiogito and loxoprofen compared to the loxoprofen group. In the evaluation using SF-36, significant improvements were found in the scores in both groups in physical functioning after 12 weeks. The amount of joint fluid was significantly decreased at 4, 8 and 12 weeks compared to pre-administration baseline in the group using Boiogito and loxoprofen. A side effect of Boiogito, dry mouth, was found in one case. The symptom was mild and improved immediately after discontinuation of administration.Conclusion: The results indicated that Boiogito have a possibility for a treatment modality for joint effusion with osteoarthritis of the knee. © 2012 Majima et al licensee BioMed Central Ltd.
• Risk of Deep Venous Thrombosis in Drain Clamping With Tranexamic Acid and Carbazochrome Sodium Sulfonate Hydrate in Total Knee Arthroplasty

  Tomohiro Onodera, Tokifumi Majima, Naohiro Sawaguchi, Yasuhiko Kasahara, Takayuki Ishigaki, Akio Minami

  JOURNAL OF ARTHROPLASTY 27 1 105 - 108 2012年01月 [査読有り][通常論文]
   

  The aim of this randomized prospective study was to clarify risks associated with a drain-clamping method using tranexamic acid and carbazochrome sodium sulfonate hydrate after total knee arthroplasty (TKA). Subjects comprised 100 patients scheduled to undergo TKA, randomized into 2 groups: 50 patients received the drain-clamping method using tranexamic acid and carbazochrome sodium sulfonate hydrate and 50 patients received drain-clamping with saline. Although bleeding volume was significantly lower in the group with tranexamic acid and carbazochrome sodium sulfonate hydrate, risk of asymptomatic deep venous thrombosis as detected by ultrasonography was comparable between groups. Tranexamic acid and carbazochrome sodium sulfonate hydrate in the drain-clamping method help reduce bleeding after TKA without increasing the risk of deep venous thrombosis.
• Alterations of High-Mannose Type N-Glycosylation in Human and Mouse Osteoarthritis Cartilage

  Atsushi Urita, Tomoya Matsuhashi, Tomohiro Onodera, Hiroaki Nakagawa, Megumi Hato, Maho Amano, Naoki Seito, Akio Minami, Shin-Ichiro Nishimura, Norimasa Iwasaki

  ARTHRITIS AND RHEUMATISM 63 11 3428 - 3438 2011年11月 [査読有り][通常論文]
   

  Objective. The process of N-glycosylation is involved in the pathogenesis of various diseases. However, little is known about the contribution of changes in N-glycans in osteoarthritis (OA). The aim of this study was to identify the alterations in N-glycans in human OA cartilage, to characterize the messenger RNA (mRNA) expression of N-glycan biosynthesis enzyme genes (N-glycogenes) in mouse articular chondrocytes during cartilage degradation, and to analyze the relationship between altered N-glycan patterns and mechanisms of cartilage degradation. Methods. Alterations in N-glycans were analyzed in human OA cartilage and degraded mouse cartilage by high-performance liquid chromatography and mass spectrometry. N-glycogene mRNA expression in mouse chondrocytes was measured using reverse transcription-polymerase chain reaction. To assess the relationship between the altered N-glycans and degradation of mouse cartilage, experiments involving either knockdown or overexpression of N-glycogenes were performed in mouse articular chondrocytes. Results. Alterations in high-mannose type N-glycans were observed in both human OA cartilage and degraded mouse cartilage. The expression of beta 1,2N-acetylglucosaminyltransferase I (GlcNAc-TI) mRNA, which converts high-mannose type N-glycans, was significantly increased in degraded mouse cartilage. Mouse chondrocytes with suppressed GlcNAc-TI expression had reduced levels of matrix metalloproteinase 13 (MMP-13) and ADAMTS-5 (aggrecanase 2) mRNA following stimulation with interleukin-1 alpha (IL-1 alpha). In contrast, mouse chondrocytes overexpressing GlcNAc-TI had increased levels of MMP-13 and ADAMTS-5 mRNA following stimulation with IL-1 alpha. Conclusion. These findings indicate that alterations in high-mannose type N-glycans and N-glycogenes in chondrocytes correlate with the release of MMP-13 and ADAMTS-5 during cartilage degradation. These findings suggest that N-glycans play a crucial role in the initiation and progression of OA.
• Metal on metal THA後にALVALを生じた1例

  清水 智弘, 井上 正弘, 沢口 直弘, 渡部 琢哉, 笠原 靖彦, 小野寺 智洋, 高橋 大介, 安藤 亮, 三浪 明男, 春藤 基之, 菅野 大己, 増田 武志

  北海道整形災害外科学会雑誌 52 2 348 - 348 北海道整形災害外科学会 2011年03月
• Btbd7 Regulates Epithelial Cell Dynamics and Branching Morphogenesis

  Tomohiro Onodera, Takayoshi Sakai, Jeff Chi-feng Hsu, Kazue Matsumoto, John A. Chiorini, Kenneth M. Yamada

  SCIENCE 329 5991 562 - 565 2010年07月 [査読有り][通常論文]
   

  During embryonic development, many organs form by extensive branching of epithelia through the formation of clefts and buds. In cleft formation, buds are delineated by the conversion of epithelial cell-cell adhesions to cell-matrix adhesions, but the mechanisms of cleft formation are not clear. We have identified Btbd7 as a dynamic regulator of branching morphogenesis. Btbd7 provides a mechanistic link between the extracellular matrix and cleft propagation through its highly focal expression leading to local regulation of Snail2 (Slug), E-cadherin, and epithelial cell motility. Inhibition experiments show that Btbd7 is required for branching of embryonic mammalian salivary glands and lungs. Hence, Btbd7 is a regulatory gene that promotes epithelial tissue remodeling and formation of branched organs.
• 人工股関節置換術術後に偽腫瘍を生じた1例

  清水 智弘, 井上 正弘, 渡部 琢哉, 沢口 直弘, 笠原 靖彦, 小野寺 智洋, 高橋 大介, 安藤 亮, 三浪 明男, 眞島 任史, 武田 直樹

  日本整形外科学会雑誌 84 6 S920 - S920 (公社)日本整形外科学会 2010年06月
• Cleft Formation and Branching morphogenesis of Salivary Gland: Exploration of New Functional Genes.

  Sakai T, Onodera T, Yamada KM

  Interface Oral Health Science 2009 1 13 - 19 2010年 [査読有り][通常論文]
  
• Local Upregulation of Stromal Cell-Derived Factor-1 After Ligament Injuries Enhances Homing Rate of Bone Marrow Stromal Cells in Rats

  Kazumi Shimode, Norimasa Iwasaki, Tokifumi Majima, Tadanao Funakoshi, Naohiro Sawaguchi, Tomohiro Onodera, Akio Minami

  TISSUE ENGINEERING PART A 15 8 2277 - 2284 2009年08月 [査読有り][通常論文]
   

  In the present study the local expression of stromal cell-derived factor-1 (SDF-1) after ligament injury and correlated change in the homing rate of systemically induced bone marrow stromal cells (BMSCs) to the injured site were clarified using a rat medial collateral ligament (MCL) injury model. SDF-1 was temporarily upregulated peaking at 2 weeks after injury. Correlated with the alteration in SDF-1 expression, the homing rate of systemically induced BMSCs was temporarily upregulated peaking at 2-4 weeks after injury. The SDF-1 expression in the MCL seems to play a crucial role in cell homing. In addition, SDF-1 did not influence the BMSCs behavior in vitro in terms of the proliferation, adhesivity, and expression of ligament fibroblast markers. The cell-based therapy for ligament and tendon injury with reference to local expression of SDF-1 may be one of the available applications.
• Embryonic organ culture.

  Takayoshi Sakai, Tomohiro Onodera

  Current protocols in cell biology Chapter 19 Unit 19.8  2008年12月 [査読有り][通常論文]
   

  This unit provides detailed protocols for dissecting embryonic organs and performing organ culture to study questions in developmental biology. Procedures are described here for dissecting organs such as kidney, lung, and salivary gland. The unit also contains commentary including troubleshooting for embryonic organ culture.
• Patterned Immobilization of Unprotected Carbohydrates on an Aminooxy Polymer-Grafted Solid Surface

  Ryosuke Kamitani, Kenichi Niikura, Tomohiro Onodera, Norimasa Iwasaki, Hideyuki Shimaoka, Kuniharu Ijiro

  Bulletin of the Chemical Society of Japan 80 9 433 - 439 Chemical Society of Japan 2007年08月 [査読有り][通常論文]
  
• Bone marrow stromal cells act as feeder cells for tendon fibroblasts through soluble factors

  Kazumi Shimode, Norimasa Iwasaki, Tokifumi Majima, Tadanao Funakoshi, Naohiro Sawaguchi, Tomohiro Onodera, Akio Minami

  TISSUE ENGINEERING 13 2 333 - 341 2007年02月 [査読有り][通常論文]
   

  Feeder effects of bone marrow stromal cells (BMSCs) on tendon fibroblasts were investigated using a coculture method for the application of ligament or tendon tissue engineering and cell therapy. BMSCs had significant effects on enhancing cell proliferation, the ability of cells to migrate, and cell adhesivity but little effect on the extracellular matrix (ECM) synthesis of tendon fibroblasts without cell-cell contact. Furthermore, the conditioned medium from BMSCs, despite the existence of fibroblasts, significantly increased the number of fibroblasts. Based on these results, the mechanism of the feeder effects is considered to be a certain signal of soluble factors from BMSCs to the fibroblasts. Comparative proteome analysis of the conditioned medium from co-culture of fibroblasts and BMSCs revealed less expression of plasminogen, which showed inhibitory effects on fibroblast proliferation. With regard to the relationships between plasminogen and BMSCs in the co-culture system, we speculate that BMSCs allow resolution of plasminogen or its cleavage activity in the medium via some mechanism.
• Specific cell behavior of human fibroblast onto carbohydrate surface detected by glycoblotting films

  Tomohiro Onodera, Kenichi Niikura, Norimasa Iwasaki, Noriko Nagahori, Hideyuki Shimaoka, Ryusuke Kamitani, Tokifumi Majima, Akio Minami, Shin-Ichiro Nishimura

  BIOMACROMOLECULES 7 11 2949 - 2955 2006年11月 [査読有り][通常論文]
   

  We synthesized an aminooxyl polymer that is reactive with the reduced end of carbohydrates using our sugar-displaying approach. The carbohydrates were easily immobilized on the polymer film (glycoblotting film) by simple immersion in a in sugar solution through stable oxime bond. The in vitro behaviors of human fibroblasts on the carbohydrate-coated surface were investigated. The adhesion of human fibroblasts on the cellobiose- and cellotriose-coated surfaces was much greater than on the other coated surfaces and the noncoated surface. This result indicated that simple structural differences in carbohydrates induced biological changes in human cells, especially cell adhesion. Our approach provides a high-throughput assay system for carbohydrate-related cell adhesion and proliferation.
• Extracellular matrix modulates expression of cell-surface proteoglycan genes in fibroblasts

  Naohiro Sawaguchi, T. Majima, N. Iwasaki, T. Funakoshi, K. Shimode, T. Onodera, A. Minami

  Connective Tissue Research 47 3 141 - 148 2006年05月 [査読有り][通常論文]
   

  Cell-surface proteoglycans are involved in many functions, including interactions with components of the extracellular microenvironment. They also act as coreceptors that bind and modify the actions of various growth factors, cytokines, and the extracellular matrix (ECM). This study investigated the regulation by the ECM of the expression of cell-surface proteoglycans (CD44, syndecan-1-4, betaglycan, glypican-1). We examined the changes in the expression levels of cell-surface proteoglycan genes in intact tendon, monolayer culture, and under various culture conditions. There was a significant increase in the expression of CD44 and syndecan-4 mRNAs during cell isolation from the tendon. With the switch to a 3D culture environment, there was a significant increase in the expression of CD44 at each passage point relative to its expression in 2D at those passage points. Syndecan-4 mRNA also increased steadily at each passage point in 3D culture environment. This influence on cell surface proteoglycans gene expression may indicate that collagen gel culture mimics in vivo tendon environment. This study provides further insight into the regulation of cell-surface proteoglycans in ligament and tendon fibroblasts by the ECM and 3D culture conditions. Copyright © Taylor & Francis Group, LLC.
• High density sugar-displaying materials using the glycoblotting methods for cell patterning

  Ryosuke Kamitani, Kenichi Niikura, Tomohiro Onodera, Norimasa Iwasaki, Masataka Fumoto, Hideyuki Shimaoka, Kuniharu Ijiro, Shin-Ichiro Nishimura

  Polymer Preprints, Japan 55 1 1895  2006年 

  Synthetic sugar-based materials have been interested in important tools, because cell surface carbohydrates are involved in numerous biological functions, including cellular recognition, adhesion, cell growth regulation. In this study, we present novel sugar-displaying materials by using the glycoblotting method combined with photo-patterning. The glycoblotting method is based on the chemoselective ligation of carbohydrates with reactive aminooxyl groups attached to the polymer side chains. Cell patternings were created on the sugar-displaying substrate by the specific cell-carbohydrate interactions.

MISC

• 遠隔転移を有する原発性悪性骨腫瘍において原発巣切除は生存を改善するか? 米国SEERデータベースを用いた研究

  福田 龍一, 松岡 正剛, 小野寺 智洋, 岩崎 浩司, 江畑 拓, 濱崎 雅成, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 98 (2) S94 -S94 2024年03月
• 内側型変形性膝関節症および膝蓋大腿関節症に対する組み合わせ式二顆置換術(BiKA)の術後10年成績

  馬場 力哉, 大越 康充, 前田 龍智, 岩館 茜, 岩崎 浩司, 小野寺 智洋, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 98 (2) S168 -S168 2024年03月
• 農業従事者における変形性膝関節症に対する手術加療の短期成績 膝周囲骨切り術と人工膝関節全置換術の比較

  上徳 善太, 横山 慎, 小林 悠人, 下田 康平, 福井 隆史, 太田 光俊, 本宮 真, 安井 啓悟, 小野寺 智洋, 岩崎 倫政 日本整形外科学会雑誌 98 (2) S190 -S190 2024年03月
• 足部に発生した原発性悪性骨腫瘍の生存解析 SEER databaseを用いた研究

  松岡 正剛, 小野寺 智洋, 岩崎 浩司, 濱崎 雅成, 江畑 拓, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 98 (2) S277 -S277 2024年03月
• 脛骨関節面の骨密度分布に半月板逸脱と下肢アライメントが与える影響

  佐藤 知哉, 岩崎 浩司, 濱崎 雅成, 鈴木 裕貴, 松岡 正剛, 小野寺 智洋, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 98 (2) S399 -S399 2024年03月
• 80歳以上高齢者に発生した四肢発生軟部肉腫における放射線照射

  松岡 正剛, 小野寺 智洋, 岩崎 浩司, 濱崎 雅成, 江畑 拓, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 98 (2) S415 -S415 2024年03月
• 女性乳がんサブタイプ分類が骨転移症例における生存へ与える影響 米国SEERデータベースを用いた研究

  松岡 正剛, 木津谷 菜悠, 小野寺 智洋, 横田 勲, 岩崎 浩司, 濱崎 雅成, 江畑 拓, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 98 (2) S417 -S417 2024年03月
• コラーゲン膜を用いた自家培養軟骨移植の臨床成績 骨膜被覆との比較

  甲斐原 拓真, 近藤 英司, 濱崎 雅成, 江畑 拓, 原 健人, 岩崎 浩司, 松岡 正剛, 小野寺 智洋, 門間 太輔, 岩崎 倫政 日本整形外科学会雑誌 98 (2) S448 -S448 2024年03月
• 外反膝に対して行った内側閉鎖式大腿骨遠位内反骨切り術の成績と膝蓋大腿関節のバイオメカニクスに与える影響

  濱崎 雅成, 松岡 正剛, 岩崎 浩司, 鈴木 裕貴, 小野寺 智洋, 岩崎 倫政, 近藤 英司, 井上 雅之, 八木 知徳, 安田 和則 日本関節病学会誌 43 (1) 63 -71 2024年03月
• エビデンスに基づくCKD診療ガイドライン2023

  丸山 彰一, 神田 英一郎, 久米 真司, 猪阪 善隆, 石倉 健司, 臼井 丈一, 内田 啓子, 岡田 浩一, 今田 恒夫, 斎藤 知栄, 鈴木 仁, 田中 哲洋, 坪井 直毅, 中川 直樹, 西尾 妙織, 深水 圭, 本田 浩一, 升谷 耕介, 横山 啓太郎, 和田 淳, 和田 隆志, 和田 健彦, 淺沼 克彦, 旭 浩一, 阿部 雅紀, 石本 卓嗣, 川浪 大治, 駒場 大峰, 佐田 憲映, 祖父江 理, 仲谷 慎也, 中司 敦子, 日比野 聡, 藤井 秀毅, 星野 純一, 細島 康宏, 前嶋 明人, 丸山 之雄, 森山 能仁, 安田 日出夫, 安田 宜成, 山本 卓, 石川 英二, 市川 大介, 伊藤 健太, 岩下 山連, 上田 誠二, 上田 裕之, 上村 治, 江里口 雅裕, 大島 恵, 大矢 昌樹, 岡本 孝之, 小口 英世, 小野寺 正輝, 貝藤 裕史, 忰田 亮平, 片山 鑑, 金子 佳代子, 上條 祐司, 神谷 雅人, 唐澤 一徳, 川口 武彦, 川嶋 聡子, 神田 祥一郎, 菅野 義彦, 菊池 洋平, 木原 正夫, 金口 翔, 栗田 宜明, 桑原 頌治, 桑原 孝成, 小泉 賢洋, 河野 圭志, 小坂 志保, 後藤 俊介, 坂口 悠介, 佐藤 隆太, 佐藤 涼介, 座間味 亮, 重冨 奈穂子, 柴田 茂, 島袋 渡, 清水 さやか, 新家 俊郎, 杉本 圭相, 杉本 俊郎, 孫 大輔, 高井 奈美, 田口 博基, 竹内 裕紀, 辰元 為仁, 田中 健一, 田邉 起, 田村 功一, 辻 章志, 辻田 誠, 寺野 千香子, 遠山 直志, 戸田 晋, 永井 恵, 中沢 大悟, 長洲 一, 中野 敏昭, 長浜 正彦, 中屋 来哉, 西 健太朗, 西脇 宏樹, 延山 理恵, 花房 規男, 濱崎 祐子, 濱田 陸, 樋口 一世, 深町 大介, 藤井 直彦, 藤崎 毅一郎, 程内 栄子, 本田 崇, 毎熊 政行, 松木 孝樹, 三浦 健一郎, 三崎 太郎, 水野 智博, 三村 洋美, 宮本 聡, 宮脇 義亜, 牟田 久美子, 村田 智博, 谷澤 雅彦, 柳原 剛, 矢野 裕一朗, 山岸 昌一, 横井 秀基, 吉崎 健, 脇 大輔, 渡邊 博志, 渡辺 博文, 渡辺 昌文, 朝比奈 悠太, 畔上 達彦, 飯田 倫理, 井口 昭, 井口 智洋, 井熊 大輔, 石井 輝, 石塚 喜世伸, 泉 裕一郎, 板野 精之, 市川 大介, 市川 一誠, 伊藤 雄伍, 伊藤 辰将, 内田 大介, 大熊 輝之, 大田 南欧美, 大西 康博, 大野 祥子, 大畑 拓也, 大山 勝宏, 岡 香奈子, 岡 樹史, 緒方 浩顕, 小田 圭子, 小田 直樹, 小原 由紀, 梶保 祐子, 梶本 幸男, 片桐 大輔, 蒲澤 秀門, 神吉 智子, 亀井 啓太, 川口 祐輝, 河原崎 宏雄, 木村 浩, 工藤 光介, 黒岡 直子, 桑形 尚吾, 高上 紀之, 古志 衣里, 近藤 悠希, 齋木 良介, 齋藤 友広, 齋藤 浩孝, 佐々木 彰, 佐藤 浩司, 猿渡 淳二, 志田 龍太郎, 菅原 亮佑, 鈴木 克彦, 諏訪部 達也, 平 大樹, 高士 祐一, 武田 尚子, 武田 有記, 田中 茂, 田中 祥子, 谷口 美紗, 塚本 俊一郎, 鶴田 悠木, 寺下 真帆, 土井 洋平, 徳永 孝史, 泊 弘毅, 鳥越 健太, 内藤 順子, 中井 健太郎, 長岡 由女, 中川 詩織, 中川 輝政, 中島 章雄, 中島 悠里, 永田 大, 永野 伸郎, 中村 祐貴, 永山 泉, 西沢 慶太郎, 西堀 暢浩, 忍頂寺 毅史, 服部 洸輝, 花井 豪, 濱田 昌実, 原田 真, 春原 浩太郎, 平井 健太, 平林 陽介, 福田 俊悟, 藤澤 諭, 藤丸 拓也, 堀越 慶輔, 本城 保菜美, 松尾 浩司, 丸山 啓介, 宮内 隆政, 宮崎 絋平, 武藤 正浩, 村島 美穂, 矢野 彰三, 山内 壮作, 山口 哲志, 山田 俊輔, 山原 康佑, 山本 脩人, 山脇 正裕, 湯浅 貴博, 吉田 学郎, 芦村 龍一, 若林 華恵, 若松 拓也, 渡邉 公雄, 渡邉 健太郎, 渡邉 周平, 小杉 智規, 日本腎臓学会 日本腎臓学会誌 66 (1) i,1 -259 2024年01月
• 変形性膝関節症の重症度別外的膝関節モーメント成分の寄与率変化の検討

  鈴木 裕貴, 小野寺 智洋, 岩崎 倫政, 大越 康充, 前田 龍智, 川上 健作, 清水 健太, 千田 周也, 浮城 健吾, 岩崎 浩司, 鈴木 昭二, 近藤 英司 日本関節病学会誌 42 (4) 340 -346 2023年12月 

  目的:内側型変形性膝関節症(KOA:knee osteoarthritis)の運動力学的特徴に関しては,いまだ解明されていない部分が多い。本研究の目的は,重症度別モーメント寄与率の特徴を明らかにすることである。方法:当院で歩行解析を実施し得たKOA症例77例93膝(北大KOA重症度分類;Stage II:19例22膝,Stage III:26例30膝,Stage IV:17例24膝,Stage V:15例19膝)を対象としてStageごとに群分けした。全症例に対し,膝関節可動域,歩行速度,単純X線所見を評価した。また,ポイントクラスター法に準じた光学式モーションキャプチャー技術と逆動力学計算により,床反力ピーク時における外的膝関節モーメントの総量(TJM:total joint moment)とそれに対する各モーメントの寄与率を求めた。結果:歩行速度と膝屈曲可動域はKOAの重症例ほど有意に小さかった(P<0.05)。KOA重症例では,femorotibial angleは大きく,TJMは大きく,TJM第一ピーク時における各モーメント寄与率は,内反モーメント(KAM:knee adduction moment)の割合が有意に大きく,屈曲モーメント(KFM:knee flexion moment)の割合が有意に小さかった。考察:KOAの重症例においては,高度の膝内反変形による長いレバーアームがKAMの寄与率が大きい要因と考えられた。また,膝屈曲可動域とKFMの寄与率が低いことから,大腿四頭筋力と膝関節機能の不良が示唆された。(著者抄録)
• 【変形性関節症の病態に迫る-疾患修飾による治療を展望して-】変形性関節症発症機序の解明に向けた軟骨変性制御機構における糖鎖機能解析

  松岡 正剛, 小野寺 智洋, 岩崎 倫政 整形・災害外科 66 (12) 1459 -1463 2023年11月 

  ＜文献概要＞変形性関節症において,関節軟骨の病態進行と関連してN型糖鎖の変化が生じ,関節軟骨の恒常性維持において糖脂質が重要な役割を果たすことも報告されている。特にガングリオシドと呼ばれる糖脂質のサブグループが関節軟骨に豊富に存在していることが明らかにされている。さらに,関節軟骨修復過程の研究においても糖脂質の重要性が示されており,一部の糖脂質の欠損により関節軟骨の修復が促進されることが報告されている。これらの研究結果から,糖鎖修飾や糖脂質の制御が変形性関節症の治療標的となる可能性があることが示唆されている。
• 遺伝性低リン血症性くる病患者に対するブロスマブ投与による下肢アライメント改善効果の検討

  坂井 裕子, 小野寺 智洋, 松岡 正剛, 中村 明枝, 岩崎 倫政 日本小児整形外科学会雑誌 32 (3) S111 -S111 2023年11月
• 【整形外科外来Red Flags 2023】膝関節痛のRed Flags 非外傷

  小野寺 智洋 Orthopaedics 36 (10) 205 -211 2023年10月 

  膝関節痛は,整形外科医が日常で最もよく見かける症状のひとつである.外傷や変形性関節症などのcommon situationsが大多数を占めるなかで,外傷に起因しない膝関節痛の頻度は,変形性関節症を除くとさほど多くはない.しかし,そのなかには重篤な後遺症や合併症を引き起こす病態が隠れているため,診察に当たっては,明確で論理的な戦略の元に理学所見を取り,また検査をオーダーしなければならない.また,確定診断に至るためには,鑑別診断の列挙・疫学的知識・危険度に応じた検査の優先順位などを瞬時に想像できる.臨床医としての総合力が求められる.そこで本稿では,日常外来診療で遭遇する可能性のある非外傷性の膝関節痛において,特に注意が必要な病態を症状別に列挙し,その疫学的特徴や診断に至るキーポイントを中心に述べる.(著者抄録)
• 重症頭部外傷慢性期患者の口腔内特徴についての放射線学的検証

  児島 美智子, 安中 恵, 吹野 茉里奈, 村上 久美子, 八巻 智洋, 小野寺 晋志, 伊東 大祐, 小瀧 勝, 小林 繁樹 The Japanese Journal of Rehabilitation Medicine 60 (秋季特別号) S504 -S504 2023年10月
• 広範な膝軟骨損傷に対するアテロコラーゲンを用いた自家培養軟骨移植術後の活動レベルの変化

  甲斐原 拓真, 近藤 英司, 小野寺 智洋, 門間 太輔, 岩崎 倫政 日本臨床スポーツ医学会誌 31 (4) S300 -S300 2023年10月
• 四肢原発悪性骨腫瘍症例における患肢温存術と切断術の比較 米国SEERデータベースを用いた研究

  柳澤 那由他, 松岡 正剛, 小野寺 智洋, 岩崎 浩司, 鈴木 裕貴, 濱崎 雅成, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 97 (8) S1887 -S1887 2023年08月
• 診断時遠隔転移を有する四肢発生原発性悪性骨腫瘍の原発巣切除が生存に与える影響 米国SEERデータベースを用いた研究

  福田 龍一, 松岡 正剛, 小野寺 智洋, 岩崎 浩司, 鈴木 裕貴, 濱崎 雅成, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 97 (8) S1888 -S1888 2023年08月
• 遠隔転移を有する軟部肉腫症例において原発巣切除は生存を延長する SEERデータベースを用いた研究

  松岡 正剛, 小野寺 智洋, 横田 勲, 岩崎 浩司, 松原 新史, 菱村 亮介, 近藤 英司, 岩崎 倫政 北海道整形災害外科学会雑誌 64 (2) 49 -58 2023年03月 

  【目的】本研究の目的は,遠隔転移を有する四肢発生軟部肉腫(Soft tissue sarcoma:STS)症例について,原発巣切除が生存を延長するか否かを明らかにし,さらに原発巣切除を考慮すべき患者群を明らかにすることである.【方法】SEERデータベースを用いて,1983年から2016年の間に初診時に遠隔転移を有する四肢発生STS1453例を抽出した.そのうち原発巣切除を施行された患者は898名,されなかった症例は555名であった.傾向スコアマッチングにより患者背景を調整後,804名を解析対象とし切除群では生存が延長した(Cancer specific survival(CSS):Hazard ratio[HR]=0.59,95% confidence interval[CI]0.50-0.71;Overall survival[OS]:HR=0.60,95% CI 0.60-0.71).サブクラス解析では,病理学的高悪性度腫瘍や未分化多形肉腫,平滑筋肉腫,滑膜肉腫症例において,原発巣切除は生存を改善した(高悪性度;CSS:HR=0.57,95% CI 0.45-0.72,OS:HR=0.58,95% CI 0.48-0.71,未分化多形肉腫;CSS:HR=0.60,95% CI 0.42-0.84,OS:HR=0.61,95% CI 0.46-0.82,平滑筋肉腫;CSS:HR=0.50,95% CI 0.33-0.75,OS HR=0.50,95% CI 0.35-0.72,滑膜肉腫;CSS:HR=0.46,95% CI 0.31-0.68,OS:HR=0.43,95% CI 0.30-0.62).【結語】本研究は,遠隔転移を有する四肢発生STS症例において原発巣切除術が生存を延長することを示した.今後,本研究結果を追試するための前向き臨床試験が必要である.(著者抄録)
• 大腿骨顆間外側壁における骨密度分布-前十字靱帯の大腿骨付着部に関する考察-

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• Protective Role of Optineurin Against Joint Destruction in Rheumatoid Arthritis Synovial Fibroblasts.

  Wen Shi Lee, Masaru Kato, Eri Sugawara, Michihiro Kono, Yuki Kudo, Michihito Kono, Yuichiro Fujieda, Toshiyuki Bohgaki, Olga Amengual, Kenji Oku, Shinsuke Yasuda, Tomohiro Onodera, Norimasa Iwasaki, Tatsuya Atsumi Arthritis & rheumatology (Hoboken, N.J.) 72 (9) 1493 -1504 2020年09月 [査読無し][通常論文]
   

  OBJECTIVE: Optineurin (OPTN) is an autophagy adaptor/receptor that acts as an intrinsic negative regulator of osteoclast differentiation. RANKL expressed by rheumatoid arthritis synovial fibroblasts (RASFs) is primarily responsible for the development of bone erosions in patients with RA. The aim of the present study was to explore the role of OPTN in the pathogenesis of joint destruction in RA. METHODS: RASFs were left untreated or incubated with tumor necrosis factor (TNF) or interferon-γ (IFNγ), and expression of OPTN by RASFs was analyzed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blotting. Expression of RANKL and osteoprotegerin (OPG) was evaluated in cultures of OPTN-reduced RASFs with or without TNF or IFNγ treatment. OPTN-reduced RASFs were cocultured with monocytes and stained for tartrate-resistant acid phosphatase (TRAP). IκBα, NF-κB1, and RelA protein levels were measured to evaluate NF-κB signaling. Expression of messenger RNA (mRNA) for matrix metalloproteinase 3 (MMP3), interleukin-6 (IL6), GATA binding protein 3 (GATA3), carbohydrate sulfotransferase 15 (CHST15), hyaluronan synthase 1 (HAS1), and GATA1 was analyzed by RT-qPCR. RESULTS: In RASFs incubated with TNF or IFNγ, OPTN expression was up-regulated and RANKL expression was increased, and these effects were further pronounced in OPTN-reduced RASFs (all P < 0.05 versus controls). OPG mRNA levels remained unchanged. Monocytes cocultured with OPTN-reduced RASFs differentiated to a greater extent into TRAP+ multinucleated cells compared to monocytes cocultured with control RASFs (P < 0.05). IκBα degradation and nuclear NF-κB1 expression following TNF treatment were both prolonged in OPTN-reduced RASFs (each P < 0.05 versus controls). MMP3 mRNA levels were up-regulated, while GATA3, CHST15, and HAS1 mRNA levels were down-regulated in OPTN-reduced RASFs (each P < 0.05 versus controls). CONCLUSION: OPTN plays a protective role in RA when it is up-regulated in RASFs in the presence of proinflammatory cytokines. Absence of OPTN might worsen RA by generating a joint-destructive state, as indicated by evidence of increased RANKL expression on RASFs and subsequent osteoclast differentiation.
• Glycolipid Expression Analysis Of Chondrocyte-like Cells Derived From Human Induced Pluripotent Stem Cells (hipsc).

  Liang Xu, Tomohiro Onodera, Jun-ichi Furukawa, Hisatoshi Hanamatsu, Kentaro Homan, Kazutoshi Hontani, Takuji Miyazaki, Yuan Tian, Norimasa Iwasaki The 66th Annual Meeting of Orthopaedic Research Society (ORS) 45 (PS2-2263) 2020年02月 [査読無し][通常論文]
  
• Elimination Of Residual Ipscs From Derived Chondrocytes By Using A Cytotoxic Antibody Specifically Bound To Ips May Reduce The Risk Of Tumorigenicity.

  Takuji Miyazaki, Tomohiro Onodera, Jun-ichi Furukawa, Hisatoshi Hanamatsu, Liang Xu, Kentaro Homan, Toshisuke Kawasaki, Norimasa Iwasaki The 66th Annual Meeting of Orthopaedic Research Society (ORS) 45 (PS2-1549) 2020年02月 [査読無し][通常論文]
  
• OCT(光干渉断層計)を用いたゲル内細胞分布の3次元解析

  山口純, 小野寺智洋, 宝満健太郎, 岩崎倫政, 丸毛啓史 日本軟骨代謝学会プログラム・抄録集 33rd 2020年
• OCT(光干渉断層計)を用いたゲル内細胞分布の三次元解析

  山口純, 山口純, 小野寺智洋, 宝満健太郎, 岩崎倫政, 丸毛啓史, 斎藤充 日本整形外科学会雑誌 94 (8) 2020年
• 骨軟骨欠損に対する高純度アルギン酸ゲルと骨髄穿刺濃縮細胞併用移植の検討

  徐 亮, 瓜田 淳, 小野寺 智洋, 菱村 亮介, 宝満 健太郎, 岩崎 倫政 整形外科バイオマテリアル研究会 39th 15 2019年12月 [査読無し][通常論文]
  
• OCT（光干渉断層計）を用いたゲル内細胞分布の3次元解析

  山口 純, 小野寺 智洋, 宝満 健太郎, 岩崎 倫政 整形外科バイオマテリアル研究会 39th 5 2019年12月 [査読無し][通常論文]
  
• 半月板修復に寄与する細胞種の探求

  宝満 健太郎, 小野寺 智洋, 金 佑泳, 近藤 英司, 岩崎 倫政 整形外科バイオマテリアル研究会 39th 17 2019年12月 [査読無し][通常論文]
  
• 半月板の再生を司るのは半月板か、滑膜か? －凍結解凍法を用いた家兎半月板損傷モデルの研究 －

  宝満 健太郎, 小野寺 智洋, 金 佑泳, 近藤英司, 岩崎 倫政 第41回日本バイオマテリアル学会大会 筑波 (8) 2019年11月 [査読無し][通常論文]
  
• The Selective Removal Method of Undifferentiated iPS Cells Using Specifi c Antibody for Cartilage Regenerative Medicine.

  T. Miyazaki, T. Onodera, L. Xu, H. Hanamatsu, J. Furukawa, K. Homan, M. Hamasaki, R. Hishimura, T. Kawasaki, N. Iwasaki World Congress of International Chemical Reference Substances (ICRS) 15 (P174) 2019年10月 [査読無し][通常論文]
  
• Therapeutic Effect of Ultra-Purified Alginate gel Containing Bone Marrow Aspirate Concentrate on Osteochondral Defects in a Rabbit Model.

  L. Xu, A. Urita, T. Onodera, R. Hishimura, M. Hamasaki, D. Liang, Y. Tian, K. Homan, N. Iwasaki World Congress of International Chemical Reference Substances (ICRS) 15 (P083) 2019年10月 [査読無し][通常論文]
  
• Identification of M9 high-mannose glycan regulating hypertrophy in articular cartilage.

  K. Homan, T. Onodera, J. Furukawa, H. Hanamatsu, R. Hishimura, K. Wooyoung, M. Hamasaki, L. Xu, N. Iwasaki World Congress of International Chemical Reference Substances (ICRS) 15 (10.4.8) 2019年10月 [査読無し][通常論文]
  
• Transcriptional profi ling of murine macrophages stimulated with cartilage fragments reveals a novel mechanism for osteoarthritis.

  M. Hamasaki, M.A. Terkawi, T. Onodera, K. Homan, G. Matsumae, Y. Tian, H. Alhasan, T. Ebata, N. Iwasaki World Congress of International Chemical Reference Substances (ICRS) 15 (12.2.4) 2019年10月 [査読無し][通常論文]
  
• 軟骨再生医療への応用を標的とした未分化iPS細胞特異的除去法の検討

  宮崎拓自, 小野寺智洋, 徐亮, 花松久寿, 古川潤一, 宝満健太郎, 川嵜敏祐, 岩崎倫政 日本運動器移植・再生医学研究会要旨集 38 2019年10月 [査読無し][通常論文]
  
• M9高マンノース型糖鎖は関節軟骨における肥大化を制御する

  宝満健太郎, 小野寺智洋, 花松久寿, 古川潤一, 菱村亮介, 金佑泳, 濱崎雅成, 徐亮, 岩崎倫政 日本整形外科学会雑誌 93 (8) S1682 -S1682 2019年09月09日 [査読無し][通常論文]
  
• 軟骨破片に対するマクロファージ炎症反応の網羅的遺伝子発現解析

  濱崎雅成, TERKAWI M. Alaa, 小野寺智洋, 宝満健太郎, 徐亮, 宮崎拓自, 田園, 江畑拓, 松前元, 岩崎倫政 日本整形外科学会雑誌 93 (8) S1627 -S1627 2019年09月09日 [査読無し][通常論文]
  
• 軟骨再生医療への応用を標的とした末分化iPS細胞特異的除去法の検討

  宮崎拓自, 小野寺智洋, 徐亮, 花松久寿, 古川潤一, 宝満健太郎, 濱崎雅成, 菱村亮介, 川嵜敏祐, 岩崎倫政 日本整形外科学会雑誌 93 (8) S1664 -S1664 2019年09月09日 [査読無し][通常論文]
  
• 骨軟骨欠損に対する骨髄穿刺濃縮細胞と高純度アルギン酸ゲル併用移植の検討

  XU L, 瓜田淳, 小野寺智洋, 菱村亮介, 濱崎雅成, 梁大偉, 宝満健太郎, 岩崎倫政 日本整形外科学会雑誌 93 (8) S1665 -S1665 2019年09月09日 [査読無し][通常論文]
  
• アテロコラーゲンを用いた自家培養軟骨移植の組織学的検討

  甲斐原 拓真, 近藤 英司, 岩崎 浩司, 小野寺 智洋, 遠山 晴一, 岩崎 倫政, 安田 和則 日本整形外科スポーツ医学会雑誌 39 (4) 469 -469 2019年08月 [査読無し][通常論文]
  
• 膝十字靱帯不全を伴う内側型変形性膝関節症に対する膝十字靱帯再建術と高位脛骨骨切り術の二期的手術の短期成績

  清水 寛和, 岩崎 浩司, 近藤 英司, 小野寺 智洋, 新井 隆太, 上徳 善太, 岩崎 倫政 JOSKAS 44 (4) 252 -252 2019年05月 [査読無し][通常論文]
  
• 両膝に発生した内側円板状半月板の2例

  青木 佑介, 岩崎 浩司, 近藤 英司, 珍部 正嗣, 新井 隆太, 小野寺 智洋, 安田 和則, 岩崎 倫政 JOSKAS 44 (2) 336 -337 2019年04月 [査読無し][通常論文]
   

  両膝内側円盤状半月板の10歳男児症例2例を呈示した。症例1は右膝痛と膝ロッキング症状、症例2は左膝痛と伸展制限を主訴とし、MRI所見より両膝完全型内側円板状半月板と診断した。2例ともに形成的半月板部分切除を行った。症例1は術後半年時点で症状は消失した。無症状の左膝は経過観察とした。症例2は2年後に右膝にも症状が出現したため同様の手術を行った。術後10年時点で症状の再発は認めない。
• 人工膝関節全置換術後インプラント周囲感染に対する治療成績の検討

  新井隆太, 小野寺智洋, 岩崎浩司, 近藤英司, 岩崎倫政 JOSKAS 44 (1) 242‐243 2019年03月25日 [査読無し][通常論文]
  
• 網羅的遺伝子発現解析によるマクロファージに対する軟骨破片の影響の検討

  濱崎雅成, TERKAWI Alaa, 小野寺智洋, 宝満健太郎, 菱村亮介, XU Liang, 宮崎拓自, TIAN Yuan, 岩崎倫政 日本軟骨代謝学会プログラム・抄録集 32nd 118 2019年03月 [査読無し][通常論文]
  
• 半月板と滑膜の半月板組織再生への寄与度の比較研究

  KIM WooYoung, 小野寺智洋, 近藤英司, 宝満健太郎, 菱村亮介, 岩崎倫政 日本軟骨代謝学会プログラム・抄録集 32nd 88 2019年03月 [査読無し][通常論文]
  
• 軟骨破片マクロファージ共培養モデルにおける軟骨細胞の網羅的遺伝子発現解析

  TERKAWI Mohamad Alaa, 濱崎雅成, 小野寺智洋, 宝満健太郎, 菱村亮介, XU Liang, 宮崎卓司, TIAN Yuan, 岩崎倫政 日本軟骨代謝学会プログラム・抄録集 32nd 119 2019年03月 [査読無し][通常論文]
  
• Total Glycome Analysis Through Chondrocyte Hypertrophy.

  Kentaro Homan, Tomohiro Onodera, Jun-ichi Furukawa, Hisatoshi Hanamatsu, Ryosuke Hishimura, WooYoung Kim, Masanari Hamasaki, Liang Xu, Norimasa Iwasaki The 65th Annual Meeting of Orthopaedic Research Society (ORS) 44 (PS2-065) 2019年02月 [査読無し][通常論文]
  
• Which Contributes To Meniscal Regeneration, Synovium Or Meniscus? -in Vivo Rabbit Model Study With Ultrapurified Low Endotoxin Alginate (upal) Gel And Freeze-thaw Method-.

  WooYoung Kim, Tomohiro Onodera, Eiji Kondo, Mohamad A. Terkawi, Kentaro Homan, Ryosuku Hishimura, Norimasa Iwasaki The 65th Annual Meeting of Orthopaedic Research Society (ORS)(New Investigator Recognition Award (NIRA) finalist) 44 (27-154) 2019年02月 [査読無し][通常論文]
  
• 軟骨再生医療への応用を標的とした未分化iPS細胞特異的除去法の検討

  宮崎拓自, 小野寺智洋, 徐亮, 花松久寿, 古川潤一, 宝満健太郎, 川嵜敏祐, 岩崎倫政 移植(Web) 54 (4-5) 2019年
• 骨軟骨欠損に対する骨髄穿刺濃縮細胞と高純度アルギン酸ゲル併用移植の検討

  徐亮, 瓜田淳, 小野寺智洋, 菱村亮介, 濱崎雅成, 梁大偉, 宝満健太郎, 岩崎倫政 北海道整形災害外科学会 137th (8) 13 -S1665 2019年 [査読無し][通常論文]
  
• 家兎半月板部分欠損モデルにおける高純度硬化性アルギン酸ゲル移植の半月板修復に与える効果

  金佑泳, 小野寺智洋, 近藤英司, 野々山貴行, 馬場力哉, 上徳善太, 宝満健太郎, 菱村亮介, 岩崎倫政 整形外科バイオマテリアル研究会プログラム・抄録集 38th 38 2018年11月 [査読無し][通常論文]
  
• 関節リウマチ患者に対する人工膝関節置換術後深部静脈血栓症の発生頻度

  上徳善太, 小野寺智洋, 近藤英司, 岩崎浩司, 馬場力哉, 中條正英, 岩崎倫政 日本関節病学会誌 37 (3) 318 2018年10月01日 [査読無し][通常論文]
  
• 全身性エリテマトーデスにおける骨壊死部位発症頻度の検討

  佐藤恒明, 小野寺智洋, 馬場力哉, 上徳善太, 岩崎浩司, 高橋大介, 近藤英司, 藤枝雄一郎, 岩崎倫政 日本関節病学会誌 37 (3) 239 2018年10月01日 [査読無し][通常論文]
  
• 足底腱膜炎:足底腱膜炎の治療における最新の知見 足底腱膜炎に対する治療の変遷

  小野寺 智洋 日本足の外科学会雑誌 39 (Suppl.) S314 -S314 2018年10月 [査読無し][通常論文]
  
• Ilizarov創外固定器を用いた足関節固定術の中期臨床成績の検討

  小野寺 智洋, 山崎 修司, 岩崎 浩司, 上徳 善太, 馬場 力哉, 近藤 英司, 岩崎 倫政 日本足の外科学会雑誌 39 (Suppl.) S426 -S426 2018年10月 [査読無し][通常論文]
  
• 強皮症による高度皮膚硬化を合併した前足部変形の1例

  松岡 正剛, 小野寺 智洋, 高橋 要, 近藤 英司, 岩崎 倫政 日本足の外科学会雑誌 39 (Suppl.) S451 -S451 2018年10月 [査読無し][通常論文]
  
• 全身性エリテマトーデスにおける骨壊死部位発症頻度の検討

  佐藤 恒明, 小野寺 智洋, 馬場 力哉, 上徳 善太, 岩崎 浩司, 高橋 大介, 近藤 英司, 藤枝 雄一郎, 岩崎 倫政 日本関節病学会誌 37 (3) 239 -239 2018年10月 [査読無し][通常論文]
  
• 関節リウマチ患者に対する人工膝関節置換術後深部静脈血栓症の発生頻度

  上徳 善太, 小野寺 智洋, 近藤 英司, 岩崎 浩司, 馬場 力哉, 中條 正英, 岩崎 倫政 日本関節病学会誌 37 (3) 318 -318 2018年10月 [査読無し][通常論文]
  
• 膝屈筋腱ハイブリッド代用材料を用いた2束前十字靱帯再再建術の臨床成績:1束再建との比較

  岩崎浩司, 近藤英司, 珍部正嗣, 小野寺智洋, 小野寺純, 八木知徳, 安田和則, 岩崎倫政 日本整形外科スポーツ医学会雑誌 38 (4) 432 2018年08月31日 [査読無し][通常論文]
  
• 重度複合靱帯損傷に対し後外側支持機構修復後,二期的に解剖学的2束ACL・PCL同時再建術を行った一例

  細川吉暁, 近藤英司, 珍部正嗣, 岩崎浩司, 小野寺智洋, 安田和則, 岩崎倫政 日本整形外科スポーツ医学会雑誌 38 (4) 701 2018年08月31日 [査読無し][通常論文]
  
• 長母趾屈筋腱周囲の複数のphosphaturic mesenchymal tumorの局所再発により腫瘍性骨軟化症が再燃した一例

  新井隆太, 小野寺智洋, 岩崎倫政 日本足の外科学会雑誌 39 (1) 245‐249 2018年08月31日 [査読無し][通常論文]
  
• 新鮮膝重度複合靱帯損傷に対する二期的再建術の短期成績

  上徳善太, 岩崎浩司, 近藤英司, 小野寺智洋, 珍部正嗣, 馬場力哉, 安田和則, 岩崎倫政 日本整形外科スポーツ医学会雑誌 38 (4) 702 2018年08月31日 [査読無し][通常論文]
  
• 膝屈筋腱ハイブリッド代用材料を用いた遺残組織温存解剖学的2束前十字靱帯再建術の臨床成績:非温存群との年齢別の検討

  江畑拓, 近藤英司, 岩崎浩司, 珍部正嗣, 新井隆太, 小野寺智洋, 小野寺純, 安田和則, 八木知徳, 岩崎倫政 日本整形外科スポーツ医学会雑誌 38 (4) 434 2018年08月31日 [査読無し][通常論文]
  
• 膝軟骨欠損に対するアテロコラーゲンを用いた自家培養軟骨移植の短~中期成績

  甲斐原拓真, 亀田敏明, 近藤英司, 小野寺智洋, 薮内康史, 遠山春一, 安田和則, 岩崎倫政 日本整形外科スポーツ医学会雑誌 38 (4) 385 2018年08月31日 [査読無し][通常論文]
  
• ラグビーシューズに関するアンケート調査 第95回全国高等学校ラグビーフットボール大会から

  杉本和也, 杉本和也, 杉本和也, 荻内隆司, 小野寺智洋, 亀山泰, 鳥居俊, 野口英雄, 橋本健史, 橋本健史, 山門浩太郎, 吉村一朗 日本足の外科学会雑誌 39 (1) 3‐9 2018年08月31日 [査読無し][通常論文]
  
• 新規ロッキングプレートを用いた内側楔状開大式高位脛骨骨切り術後のスポーツおよび社会復帰に関する検討

  薮内康史, 近藤英司, 小野寺純, 小野寺智洋, 八木知徳, 岩崎倫政, 安田和則 日本整形外科スポーツ医学会雑誌 38 (4) 388 2018年08月31日 [査読無し][通常論文]
  
• マクロファージ共培養モデルにおいて軟骨破片が引き起こすマクロファージの炎症反応および軟骨細胞への影響

  濱崎雅成, TERKAWI Mohamad Alaa, 小野寺智洋, 宝満健太郎, 上徳善太, 松原新史, 菱村亮介, 徐亮, 宮崎拓自, 田園, 岩崎倫政 日本整形外科学会雑誌 92 (8) S2012 -S2012 2018年08月30日 [査読無し][通常論文]
  
• 自家骨軟骨柱移植術に高純度アルギン酸ゲルを併用した治療効果の検討

  菱村亮介, 小野寺智洋, 上徳善太, 宝満健太郎, 松原新史, 金佑泳, 濱崎雅成, 岩崎倫政 日本整形外科学会雑誌 92 (8) S1639 -S1639 2018年08月30日 [査読無し][通常論文]
  
• 軟骨変性と肥大分化における軟骨細胞グライコームの類似性

  宝満健太郎, 小野寺智洋, 花松久寿, 古川潤一, 上徳善太, 松原新史, 菱村亮介, 金佑泳, 濱崎雅成, 宮崎拓自, 岩崎倫政 日本整形外科学会雑誌 92 (8) S1653 -S1653 2018年08月30日 [査読無し][通常論文]
  
• ビタミンA誘導体の長期投与による早期骨端線閉鎖により両膝内反変形を認めた一例

  長谷川裕一, 小野寺智洋, 岩崎浩司, 珍部正嗣, 新井隆太, 近藤英司, 岩崎倫政 東日本整形災害外科学会雑誌 30 (3) 314 2018年08月21日 [査読無し][通常論文]
  
• 軟骨細胞肥大における総合グライコーム解析

  宝満健太郎, 花松久寿, 古川潤一, 岡田和恵, 横田育子, PIAO Jinhua, 小野寺智洋, 岩崎倫政 日本糖質学会年会要旨集 37th 123 2018年08月20日 [査読無し][通常論文]
  
• 小児Run-over injuryによる広範囲足背部組織欠損に対してcrossleg flap施行後骨成長終了までフォローし得た1例

  珍部 正嗣, 小野寺 智洋, 新井 隆太, 岩崎 浩司, 岩崎 倫政, 近藤 英司, 綛村 俊之 北海道整形災害外科学会雑誌 60 (1) 116 -116 2018年08月 [査読無し][通常論文]
  
• マウスiPS細胞からの軟骨分化誘導と糖鎖解析を用いた新規バイオマーカーの探索

  本谷 和俊, 小野寺 智洋, 寺島 理代, 門間 太輔, 松岡 正剛, 馬場 力哉, 上徳 善太, 松原 新史, 宝満 健太郎, 菱村 亮介, 金 佑泳, 濱崎 雅成, 徐 亮, 岩崎 倫政 北海道整形災害外科学会雑誌 60 (1) 145 -145 2018年08月 [査読無し][通常論文]
  
• 軟骨細胞の力学的ストレス応答におけるスフィンゴ糖脂質の機能解析

  松原 新史, 小野寺 智洋, 門間 太輔, 馬場 力哉, 本谷 和俊, 上徳 善太, 宝満 健太郎, 岩崎 倫政, 大橋 俊朗, 前田 英次郎 北海道整形災害外科学会雑誌 60 (1) 145 -146 2018年08月 [査読無し][通常論文]
  
• 遺残組織を温存した解剖学的2束前十字靱帯再建術の臨床成績 多数例における非温存群と比較

  塩田 惇喜, 近藤 英司, 小野寺 智洋, 岩崎 浩司, 珍部 正嗣, 新井 隆太, 岩崎 倫政 日本整形外科スポーツ医学会雑誌 38 (4) 434 -434 2018年08月 [査読無し][通常論文]
  
• 両側膝複合靱帯損傷に対して一期的靱帯再建術を行った一例

  鈴木 瞭太, 岩崎 浩司, 下田 康平, 小林 英之, 忠鉢 敏弥, 珍部 正嗣, 新井 隆太, 小野寺 智洋, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 60 (1) 158 -158 2018年08月 [査読無し][通常論文]
  
• TriS Medial HTO Plate Systemを用いた内側楔状開大式高位脛骨骨切り術の短期成績 新規TriS plateでの比較

  薮内 康史, 近藤 英司, 亀田 敏明, 佐藤 大, 小野寺 智洋, 岩崎 倫政, 小野寺 純, 八木 知徳, 安田 和則 北海道整形災害外科学会雑誌 60 (1) 159 -159 2018年08月 [査読無し][通常論文]
  
• 膝複合靱帯損傷を合併した脛骨高原骨折術後下肢外反変形に対してDouble Level Osteotomyを行った1例

  下田 康平, 珍部 正嗣, 小野寺 智洋, 新井 隆太, 岩崎 浩司, 小林 英之, 鈴木 瞭太, 忠鉢 敏弥, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 60 (1) 160 -160 2018年08月 [査読無し][通常論文]
  
• 軟骨細胞にはNF-κB Arthritis Inducer 1を介する炎症回路活性化機構が存在する

  太田 光俊, 田中 勇希, 有馬 康伸, 上村 大輔, 小野寺 智洋, 村上 正晃, 岩崎 倫政 北海道整形災害外科学会雑誌 60 (1) 164 -164 2018年08月 [査読無し][通常論文]
  
• 当科における人工距骨置換術の短期臨床成績

  小林 英之, 小野寺 智洋, 近藤 英司, 岩崎 浩司, 珍部 正嗣, 岩崎 倫政, 深谷 英昭 北海道整形災害外科学会雑誌 60 (1) 188 -188 2018年08月 [査読無し][通常論文]
  
• 自家骨軟骨柱移植術に高純度アルギン酸ゲル(UPALゲル)を併用した治療増強効果

  菱村 亮介, 小野寺 智洋, 馬場 力哉, 松原 新史, 上徳 善太, 近藤 英司, 岩崎 倫政 JOSKAS 43 (4) 209 -209 2018年05月 [査読無し][通常論文]
  
• 内側楔状開大式骨切り術後の脛骨の捻転変化 Multiple Planner Reconstruction-CTを用いて

  珍部 正嗣, 近藤 英司, 岩崎 浩司, 小野寺 智洋, 小野寺 純, 井上 雅之, 八木 知徳, 安田 和則, 岩崎 倫政 JOSKAS 43 (4) 293 -293 2018年05月 [査読無し][通常論文]
  
• ロッキングプレートを用いた逆V字型高位脛骨骨切り術の短期成績

  岩崎 浩司, 近藤 英司, 珍部 正嗣, 小野寺 智洋, 小野寺 純, 井上 雅之, 青木 喜満, 八木 知徳, 安田 和則, 岩崎 倫政 JOSKAS 43 (4) 396 -396 2018年05月 [査読無し][通常論文]
  
• 両膝に発生した内側円板状半月板の2例

  青木 佑介, 珍部 正嗣, 近藤 英司, 岩崎 浩司, 新井 隆太, 小野寺 智洋, 安田 和則, 岩崎 倫政 JOSKAS 43 (4) 624 -624 2018年05月 [査読無し][通常論文]
  
• 新鮮膝重度複合靱帯損傷に対する二期的再建術の短期成績

  上徳 善太, 岩崎 浩司, 近藤 英司, 小野寺 智洋, 珍部 正嗣, 安田 和則, 岩崎 倫政 JOSKAS 43 (4) 676 -676 2018年05月 [査読無し][通常論文]
  
• 骨移植を併用した2期的前十字靱帯再再建術の臨床成績

  岩崎 浩司, 近藤 英司, 珍部 正嗣, 小野寺 智洋, 小野寺 純, 八木 知徳, 安田 和則, 岩崎 倫政 JOSKAS 43 (4) 679 -679 2018年05月 [査読無し][通常論文]
  
• 膝屈筋腱ハイブリッド代用材料を用いた遺残組織温存解剖学的2束前十字靱帯再建術の再鏡視所見 非温存群との年齢別の検討

  江畑 拓, 近藤 英司, 岩崎 浩司, 珍部 正嗣, 新井 隆太, 小野寺 智洋, 小野寺 純, 安田 和則, 八木 知徳, 岩崎 倫政 JOSKAS 43 (4) 685 -685 2018年05月 [査読無し][通常論文]
  
• 人工膝関節全置換術インプラント周囲感染に対する治療成績の検討

  新井 隆太, 小野寺 智洋, 岩崎 浩司, 珍部 正嗣, 近藤 英司, 岩崎 倫政 JOSKAS 43 (4) 747 -747 2018年05月 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術における骨棘移植の効果

  徳廣 泰貴, 薮内 康史, 小野寺 純, 小野寺 智洋, 近藤 英司, 八木 知徳, 岩崎 倫政, 安田 和則 JOSKAS 43 (4) 756 -756 2018年05月 [査読無し][通常論文]
  
• 高位脛骨骨切り術が膝蓋骨高位に与える影響 内側楔状開大式と逆V字型の比較

  細川 吉暁, 近藤 英司, 珍部 正嗣, 岩崎 浩司, 小野寺 智洋, 安田 和則, 岩崎 倫政 JOSKAS 43 (4) 757 -757 2018年05月 [査読無し][通常論文]
  
• 膝屈筋腱ハイブリッド代用材料を用いた解剖学的2束前十字靱帯再建術の臨床成績 術前待機期間の影響

  馬場 力哉, 近藤 英司, 小野寺 純, 北村 信人, 小野寺 智洋, 亀田 敏明, 安田 和則, 岩崎 倫政 JOSKAS 43 (1) 112 -113 2018年03月 [査読無し][通常論文]
   

  片側膝前十字靱帯(ACL)単独損傷に対して解剖学的2重束ACL再建術を行った179例を対象に、術前待機期間の違いが臨床成績に及ぼす影響について検討した。術前待機期間の違いにより、1ヵ月以内をI群(26例)、1〜3ヵ月内をII群(77例)、3〜6ヵ月以内をIII群(41例)、6〜24ヵ月以内をIV群(35例)に分け、術後1年時の臨床成績を後ろ向きに評価した。術後の膝前方安定性は、I群0.5±1.8mm、II群1.1±1.7mm、III群1.3±1.5mm、IV群1.3±1.8mmであり、I群が良好な傾向を認めたが4群間での有意差はなかった。Pivot-shift test、Lysholm score、IKDC評価、関節可動域に関しても群間に差はなかった。内側半月板損傷の割合は、待機期間が長くなるにつれ、その割合が増加する傾向にあり、そのうち切除を要した割合も同様の傾向を示していた。
• 高純度アルギン酸ゲルを併用した骨髄刺激法の効果 ビーグル犬骨軟骨欠損モデルを用いて

  馬場 力哉, 小野寺 智洋, 本谷 和俊, 上徳 善太, 松原 新史, 宝満 健太郎, 岩崎 倫政 北海道整形災害外科学会雑誌 59 (2) 250 -251 2018年03月 [査読無し][通常論文]
  
• 高マンノース型糖鎖の変化は早期OAに類似した可逆的軟骨変性を生じさせる

  宝満 健太郎, 小野寺 智洋, 古川 潤一, 門間 太輔, 松岡 正剛, 馬場 力哉, 本谷 和俊, 上徳 善太, 松原 新史, 菱村 亮介, 金 佑泳, 濱崎 雅成, 徐 亮, 田 園, 岩崎 倫政 北海道整形災害外科学会雑誌 59 (2) 253 -253 2018年03月 [査読無し][通常論文]
  
• 軟骨細胞の力学的ストレス応答におけるスフィンゴ糖脂質の機能解析

  松原 新史, 小野寺 智洋, 門間 太輔, 馬場 力哉, 本谷 和俊, 上徳 善太, 宝満 健太郎, 岩崎 倫政, 前田 英次郎, 大橋 俊朗 北海道整形災害外科学会雑誌 59 (2) 253 -254 2018年03月 [査読無し][通常論文]
  
• The candidates of glyco-biomarker of chondrocyte hypertrophy detected by Comprehensive N-glycan profiling.

  Kentaro Homan, Tomohiro Onodera, Jun-ichi Furukawa, Masatake Matsuoka, Daisuke Momma, Rikiya Baba, Kazutoshi Hontani, Zenta Joutoku, Shinji Matsubara, Ryosuke Hishimura, Kim WooYoung, Masanari Hamasaki, Liang Xu, Yuan Tian, Norimasa Iwasaki The 64th Annual Meeting of Orthopaedic Research Society (ORS) 43 (PS2-104) 2018年03月 [査読無し][通常論文]
  
• Effects Of Mosaicplasty Augmented By Ultrapurified Alginate Ael In A Rabbit Osteochondral Defect Model.

  Ryosuke hishimura, Tomohiro Onodera, Kentaro Homan, Shinji Matsubara, Zenta Joutoku, WooYoung Kim, Masanari Hamasaki, Liang Xu, Yuan Tian, Takuji Miyazaki, Norimasa Iwasaki The 64th Annual Meeting of Orthopaedic Research Society (ORS) 43 (PS2-116) 2018年03月 [査読無し][通常論文]
  
• Depletion of Glycoshingolipids Induces the Exvessive Response of Chondrocytes under Mechanical Stress Condition.

  Shinji Matsubara, Tomohiro Onodera, Eijiro Maeda, Momma Daisuke, Masatake Matsuoka, Rikiya Baba, Kazutoshi Hontani, Zenta Joutoku, Kentaro Homan, Toshiro Ohashi, Norimasa Iwasaki The 64th Annual Meeting of Orthopaedic Research Society (ORS) 43 (19) 2018年03月 [査読無し][通常論文]
  
• CCL21/CCR7 Axis Regulating Juvenile Cartilage Repair Can Enhance Cartilage Healing In Adult.

  Zenta Joutoku, Tomohiro Onodera, Daisuke Momma, Masatake Matsuoka, Rikiya Baba, Kazutoshi Hontani, Shinji Matsubara, Ryosuke Hishimura, Norimasa Iwasaki The 64th Annual Meeting of Orthopaedic Research Society (ORS) 43 (12) 2018年03月 [査読無し][通常論文]
  
• 新規ロッキングプレートシステムを用いた内側楔状開大式高位脛骨骨切り術の短期成績

  薮内 康史, 近藤 英司, 小野寺 純, 小野寺 智洋, 放生 憲博, 八木 知徳, 岩崎 倫政, 安田 和則 日本整形外科学会雑誌 92 (2) S132 -S132 2018年03月 [査読無し][通常論文]
  
• CT osteoabsorptiometry法を用いた内側楔状開大式高位脛骨骨切り術後の膝蓋大腿関節における応力変化

  亀田 敏明, 近藤 英司, 薮内 康史, 小野寺 智洋, 小野寺 純, 八木 知徳, 安田 和則, 岩崎 倫政 日本整形外科学会雑誌 92 (2) S135 -S135 2018年03月 [査読無し][通常論文]
  
• 前十字靱帯再建術-Update- 前十字靱帯再建術における遺残組織温存の効果

  近藤 英司, 岩崎 浩司, 珍部 正嗣, 小野寺 純, 横田 正司, 小野寺 智洋, 安田 和則, 岩崎 倫政 日本整形外科学会雑誌 92 (2) S444 -S444 2018年03月 [査読無し][通常論文]
  
• 陳旧性膝重度複合靱帯損傷に対する一期的再建術の治療成績

  松原 新史, 近藤 英司, 小野寺 純, 小野寺 智洋, 亀田 敏明, 安田 和則, 岩崎 倫政 JOSKAS 43 (1) 178 -179 2018年03月 [査読無し][通常論文]
   

  陳旧性膝重度複合靱帯損傷に対して一期的に再建術を行った33例33膝(男性29例、女性4例、平均30歳)を対象に、その治療成績について検討した。手術はACLおよびPCL損傷のみの症例に対して両側から膝屈筋腱を採取し、膝屈筋腱ハイブリッド代用材料を作製した。ACL再建は経脛骨手技を、PCL再建はout-side inガイドを用いて行った。可動域は5°以上の伸展制限を認めた症例はなかったものの、8例に屈曲15°以上の制限を認めた。KT-2000またはKnee Rax3による術後膝前後不安定性評価は、屈曲20°で2.0±1.9mm、70°で2.9±2.8mmであった。ストレスX線撮影による前後不安定性評価は、前方引き出しで健側63.0±4.1%に対して患側63.2±5.3%、後方引き出しで健側57.1±4.8%に対して患側54.2±6.7%であった。IKDC評価は、A:13例、B:12例、C:5例、D:3例と比較的良好な成績であった。術前の可動域制限の改善および感染症の予防が重要であると思われた。
• Tris plateを用いた内側開大式高位脛骨骨切り術の短期成績 抜釘症例における従来のプレートとの比較

  薮内 康史, 近藤 英司, 小野寺 純, 亀田 敏明, 佐藤 大, 小野寺 智洋, 八木 知徳, 岩崎 倫政, 安田 和則 JOSKAS 43 (1) 290 -291 2018年03月 [査読無し][通常論文]
   

  2009〜2015年にlocking plateを用いた内側楔状開大式高位脛骨骨切り術(OWHTO)を行った156例167膝(男性38例40膝、女性118例127膝)を対象に、抜釘後におけるOWHTOの臨床成績を二つのplate間で比較検討した。2009〜2014年11月にTomoFixを使用した患者をF群(129例139膝)、2014年12月〜2015年にTriS plateを使用した患者をS群(27例28膝)とした。抜釘時に臨床および画像評価を行った。さらにF群24例、S群12例に対し術後CTを撮像し内固定材の位置を解析した。術後JOA scoreは両群ともに平均91点と有意に改善した。FTAは両群ともに術後170°に、下肢機能軸は約70%に矯正された。脛骨後傾角はF群で有意に増加したが、S群では変化はなかった。CTでは、S群のプレート位置はF群に比べ有意に後方設置されていた。近位スクリュー角度は、S群はF群に比べてより有意に脛骨後面に対して平行であった。術中合併症に関しては、外側ヒンジ部骨折はF群26例(19%)、S群5例(18%)で差はなかった。一方、抜釘時合併症はF群ではスクリュー折損を8例(6%)に生じていたのに対しS群では認めなかった。
• 軟部組織での再発を来した脛骨近位部骨巨細胞腫の一例

  新井 隆太, 小野寺 智洋, 河村 太介, 亀田 敏明, 籔内 康史, 下段 俊, 細川 吉暁, 岩崎 倫政, 高桑 恵美, 近藤 英司 北海道整形災害外科学会雑誌 59 (2) 269 -269 2018年03月 [査読無し][通常論文]
  
• 血友病性膝関節症に対する人工膝関節置換術の臨床成績

  横田 隼一, 小野寺 智洋, 亀田 敏明, 林 晴久, 加藤 琢磨, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 59 (2) 281 -282 2018年03月 [査読無し][通常論文]
  
• 鏡視下足関節固定術における距腿関節間距離および足関節固定角度と骨癒合期間との関係

  林 晴久, 小野寺 智洋, 亀田 敏明, 加藤 琢磨, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 59 (2) 288 -288 2018年03月 [査読無し][通常論文]
  
• 当科におけるリウマチ性前足部変形に対する第1MTP関節温存術の治療成績

  小野寺 智洋, 林 晴久, 亀田 敏明, 新井 隆太, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 59 (2) 295 -296 2018年03月 [査読無し][通常論文]
  
• 新しいロッキングプレートを用いた内側楔状開大式高位脛骨骨切り術の臨床成績 抜釘症例での検討

  薮内 康史, 小野寺 智洋, 亀田 敏明, 佐藤 大, 岩崎 倫政, 近藤 英司, 小野寺 純, 安田 和則, 八木 知徳 北海道整形災害外科学会雑誌 59 (2) 334 -334 2018年03月 [査読無し][通常論文]
  
• 高位脛骨骨切り術における深部静脈血栓症の発症率 人工膝関節全置換術との比較

  小池 良直, 亀田 敏明, 薮内 康史, 佐藤 大, 小野寺 智洋, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 59 (2) 335 -335 2018年03月 [査読無し][通常論文]
  
• 膝屈筋腱ハイブリッド代用材料を用いた解剖学的2束前・後十字靱帯同時再建術の2例

  珍部 正嗣, 下出 和美, 石田 博英, 近藤 英司, 亀田 敏明, 小野寺 智洋, 岩崎 倫政, 小野寺 純, 後藤 圭子, 安田 和則 北海道整形災害外科学会雑誌 59 (2) 344 -345 2018年03月 [査読無し][通常論文]
  
• 膝屈筋腱ハイブリッド代用材料を用いた遺残組織温存解剖学的2束前十字靱帯再建術の臨床成績 非温存群との年齢別検討

  江畑 拓, 近藤 英司, 岩崎 浩司, 珍部 正嗣, 新井 隆太, 小野寺 智洋, 小野寺 純, 安田 和則, 八木 知徳, 岩崎 倫政 日本整形外科学会雑誌 92 (3) S617 -S617 2018年03月 [査読無し][通常論文]
  
• 陳旧性膝重度複合靱帯損傷に対する自家腱ハイブリッド代用材料を用いた一期的再建術の治療成績

  松原 新史, 近藤 英司, 小野寺 純, 小野寺 智洋, 亀田 敏明, 珍部 正嗣, 北村 信人, 安田 和則, 岩崎 倫政 日本整形外科学会雑誌 92 (3) S760 -S760 2018年03月 [査読無し][通常論文]
  
• リウマチ性外反母趾変形に対するLapidus変法と近位骨切り術の臨床成績の比較

  小野寺 智洋, 林 晴久, 近藤 英司, 岩崎 浩司, 珍部 正嗣, 岩崎 倫政 日本整形外科学会雑誌 92 (3) S1029 -S1029 2018年03月 [査読無し][通常論文]
  
• 緩徐矯正術後の高度尖足に対する創外固定装着下鏡視下足関節固定術の有用性

  小野寺 智洋, 岩崎 浩司, 珍部 正嗣, 林 晴久, 深谷 英昭, 山崎 修司, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 92 (3) S1051 -S1051 2018年03月 [査読無し][通常論文]
  
• 遺残組織を温存した解剖学的2束前十字靱帯再建術の臨床成績 多数例における非温存群と比較

  塩田 惇喜, 近藤 英司, 小野寺 智洋, 岩崎 浩司, 珍部 正嗣, 小野寺 純, 安田 和則, 八木 知徳, 岩崎 倫政 日本整形外科学会雑誌 92 (3) S1205 -S1205 2018年03月 [査読無し][通常論文]
  
• 膝屈筋腱ハイブリッド代用材料を用いた一期的および二期的2束前十字靱帯再再建術の臨床成績

  岩崎 浩司, 近藤 英司, 珍部 正嗣, 小野寺 智洋, 小野寺 純, 八木 知徳, 安田 和則, 岩崎 倫政 日本整形外科学会雑誌 92 (3) S1206 -S1206 2018年03月 [査読無し][通常論文]
  
• 人工膝関節全置換術後インプラント周囲感染に対する治療成績の検討

  新井 隆太, 小野寺 智洋, 岩崎 浩司, 珍部 正嗣, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 92 (3) S1287 -S1287 2018年03月 [査読無し][通常論文]
  
• 8-plateを用いた成長抑制術を行い脚長補正に2年を要した下肢片側肥大症の1例

  甲斐原 拓真, 小野寺 智洋, 薮内 康史, 亀田 敏明, 近藤 英司, 岩崎 倫政 北海道整形災害外科学会雑誌 : 北海道整形災害外科学会機関誌 59 (2) 225 -228 2018年03月 [査読無し][通常論文]
  
• 軟骨再生研究 (誌上シンポジウム 北海道における整形外科基礎研究)

  小野寺 智洋, 岩崎 倫政 北海道整形災害外科学会雑誌 : 北海道整形災害外科学会機関誌 59 (2) 209 -212 2018年03月 [査読無し][通常論文]
  
• 基礎研究体制と概要 : 北海道大学整形外科 (誌上シンポジウム 北海道における整形外科基礎研究)

  角家 健, 近藤 英司, 高畑 雅彦, 須藤 英毅, 古川 潤一, 小野寺 智洋, テルカウイ アラー, 岩崎 倫政 北海道整形災害外科学会雑誌 : 北海道整形災害外科学会機関誌 59 (2) 197 -204 2018年03月 [査読無し][通常論文]
  
• マクロファージ共培養モデルにおいて軟骨破片が軟骨細胞に与える影響

  濱崎雅成, TERKAWI Mohamad Alaa, 小野寺智洋, 宝満健太郎, 上徳善太, 松原新史, 菱村亮介, KIM WooYoung, XU Liang, 岩崎倫政 日本軟骨代謝学会プログラム・抄録集 31st 75 2018年02月 [査読無し][通常論文]
  
• 軟骨細胞肥大分化における統合グライコミクス

  宝満健太郎, 小野寺智洋, 花松久寿, 古川潤一, 上徳善太, 松原新史, 菱村亮介, KIM WooYoung, 濱崎雅成, XU Liang, 宮崎拓自, TIAN Yuan, 岩崎倫政 日本軟骨代謝学会プログラム・抄録集 31st 94 2018年02月 [査読無し][通常論文]
  
• リウマチ性膝関節症に対するTKAではBMI,MMP‐3低値が術後感染のリスク因子となりうる

  青木佑介, 小野寺智洋, 岩崎浩司, 珍部正嗣, 新井隆太, 近藤英司, 岩崎倫政 北海道整形災害外科学会 134th 55 2018年 [査読無し][通常論文]
  
• マクロファージ共培養モデルにおいて軟骨破片が軟骨細胞に与える影響

  濱崎雅成, テルカウイ アラー, 小野寺智洋, 宝満健太郎, 上徳善太, 松原新史, 菱村亮介, 徐亮, 田園, 岩崎倫政 北海道整形災害外科学会 134th (134th suppl) 37 -37 2018年 [査読無し][通常論文]
  
• 軟骨細胞肥大分化の包括的N型糖鎖プロファイリングによる糖鎖バイオマーカー探索

  宝満健太郎, 小野寺智洋, 花松久寿, 古川潤一, 門間太輔, 松岡正剛, 馬場力哉, 本谷和俊, 上徳善太, 松原新史, 菱村亮介, 金佑泳, 濱崎雅成, 岩崎倫政 北海道整形災害外科学会 134th (134th suppl) 36 -36 2018年 [査読無し][通常論文]
  
• 骨軟骨柱移植術に高純度アルギン酸ゲル(UPALゲル)移植を併用した治療効果の検討

  菱村亮介, 小野寺智洋, 宝満健太郎, KIM Woo Yong, 濱崎雅成, XU Liang, 岩崎倫政 日本バイオマテリアル学会大会予稿集(Web) 40th 166 (WEB ONLY) -166 2018年 [査読無し][通常論文]
  
• リウマチ性足関節症に対するIlizarov創外固定器を用いた足関節固定術と鏡視下足関節固定術の比較検討

  林 晴久, 小野寺 智洋, 深谷 英昭, 岩崎 倫政, 近藤 英司, 山崎 修司 日本創外固定・骨延長学会雑誌 29 148 -148 2018年01月 [査読無し][通常論文]
  
• 重度麻痺性尖足変形に対する創外固定装着下鏡視下足関節固定術の小経験

  小野寺 智洋, 林 晴久, 深谷 英昭, 岩崎 倫政, 近藤 英司, 山崎 修司 日本創外固定・骨延長学会雑誌 29 157 -157 2018年01月 [査読無し][通常論文]
  
• 小児線状強皮症に対してシクロスポリン内服が有効であった3例

  眞井 翔子, 藤田 靖幸, 宮内 俊成, 藤村 悠, 夏賀 健, 乃村 俊史, 西江 渉, 清水 宏, 小野寺 智洋, 岡 敏明 日本皮膚科学会雑誌 128 (1) 62 -62 2018年01月 [査読無し][通常論文]
  
• TriS Medial HTO Plate Systemを用いた内側楔状開大式高位脛骨骨切り術の短期成績

  薮内 康史, 近藤 英司, 小野寺 智洋, 亀田 敏明, 佐藤 大, 岩崎 倫政, 小野寺 純, 安田 和則, 八木 知徳 日本関節病学会誌 36 (4) 427 -433 2017年12月 [査読無し][通常論文]
   

  内側型変形性膝関節症や特発性膝骨壊死に対して、locking plateを用いた内側楔状開大式高位脛骨骨切り術を施行した50例54膝(男性19例、女性31例、36〜78歳)を対象とした。JOAスコアは、平均68.9→89.0点に有意に改善した。Lysholmスコアは平均59.6→81.3点に、KOOSは、5項目いずれも術後に有意に改善した。ROMは、伸展で平均-2.3→-1.0°に有意に改善した。FTAは、平均180.2→169.7°、WBLは、平均23.8→69.1%に矯正された。CT評価では、プレートは横断面で平均29.0%の位置に設置され、近位後方のスクリュー角度と後方骨皮質とのなす角度は平均150°であった。膝窩動脈から最後方スクリューの延長線上への垂線の距離は21.5mmであった。合併症は8例(14.8%)に認め、表層感染1例、脛骨外側ヒンジ部骨折7例であった。術後1年経過した14例に抜釘を行ったが、抜釘までの間にプレートによる著しい皮膚刺激症状を認めた症例はなかった。抜釘時にスクリュー折損などの内固定材料の折損症例もなかった。
• ケモカインCCL21を用いた軟骨再生のためのインテリジェントマテリアルの開発

  上徳 善太, 小野寺 智洋, 松岡 正剛, 馬場 力哉, 本谷 和俊, 宝満 健太郎, 岩崎 倫政 日本バイオマテリアル学会大会予稿集 39回 96 -96 2017年11月13日 [査読無し][通常論文]
  
• ケモカインCCL21を用いた軟骨再生のためのインテリジェントマテリアルの開発

  上徳 善太, 小野寺 智洋, 松岡 正剛, 馬場 力哉, 本谷 和俊, 宝満 健太郎, 岩崎 倫政 日本バイオマテリアル学会大会予稿集 39回 96 -96 2017年11月 [査読無し][通常論文]
  
• 最新基礎科学 知っておきたい 高純度アルギン酸ゲルを用いた関節軟骨に対する新規治療法の開発

  小野寺 智洋, 馬場 力哉, 五十嵐 達弥, 佃 幸憲, 祐川 敦, 岩崎 倫政 臨床整形外科 52 (11) 1050 -1054 2017年11月 [査読無し][通常論文]
  
• 足趾に局所再発を来した腫瘍性骨軟化症の1例

  新井隆太, 小野寺智洋, 近藤英司, 岩崎倫政 日本足の外科学会雑誌 38 (2) S315 2017年10月11日 [査読無し][通常論文]
  
• 血友病性膝関節症に対する人工膝関節置換術の臨床成績

  横田 隼一, 小野寺 智洋, 亀田 敏明, 林 晴久, 加藤 琢磨, 近藤 英司, 岩崎 倫政 日本関節病学会誌 36 (3) 322 -322 2017年10月 [査読無し][通常論文]
  
• TriS plateを用いた内側楔状開大式高位脛骨骨切り術の短期成績 抜釘症例における従来のプレートとの比較

  薮内 康史, 近藤 英司, 小野寺 純, 竹内 博紀, 小野寺 智洋, 八木 知徳, 岩崎 倫政, 安田 和則 日本関節病学会誌 36 (3) 335 -335 2017年10月 [査読無し][通常論文]
  
• 65歳以上の高齢者に対する内側楔状開大式高位脛骨骨切り術の臨床成績

  竹内 博紀, 藪内 康史, 近藤 英司, 小野寺 純, 小野寺 智洋, 八木 知徳, 岩崎 倫政, 安田 和則 日本関節病学会誌 36 (3) 336 -336 2017年10月 [査読無し][通常論文]
  
• 両側膝複合靱帯損傷に対して一期的靱帯再建術を行った一例

  鈴木 瞭太, 近藤 英司, 忠鉢 敏弥, 下田 康平, 小林 英之, 岩崎 浩司, 珍部 正嗣, 新井 隆太, 小野寺 智洋, 岩崎 倫政 日本関節病学会誌 36 (3) 379 -379 2017年10月 [査読無し][通常論文]
  
• 麻痺性尖足変形に対する創外固定装着下鏡視下足関節固定術の臨床成績

  小野寺 智洋, 林 晴久, 深谷 英昭, 山崎 修司, 近藤 英司, 岩崎 倫政 日本足の外科学会雑誌 38 (2) S229 -S229 2017年10月 [査読無し][通常論文]
  
• 従来型MRIに対応した独自のソフトウエアを用いた膝軟骨体積の評価(Assessment of Knee Cartilage Volume Using an Original Software for Conventional MRI)

  宍戸 駿, 神島 保, 加藤 一樹, 市川 翔太, 近藤 英司, 小野寺 智洋 日本放射線技術学会雑誌 73 (9) 867 -867 2017年09月 [査読無し][通常論文]
  
• Assessment of Knee Cartilage Volume Using an Original Software for Conventional MRI(和訳中)

  宍戸 駿, 神島 保, 加藤 一樹, 市川 翔太, 近藤 英司, 小野寺 智洋 日本放射線技術学会雑誌 73 (9) 867 -867 2017年09月 [査読無し][通常論文]
  
• 関節軟骨の再生医療 CCL21/CCR7は幼若個体での軟骨修復に重要である

  上徳 善太, 小野寺 智洋, 門間 太輔, 松岡 正剛, 馬場 力哉, 本谷 和俊, 松原 新史, 宝満 健太郎, 菱村 亮介, 金 佑泳, 濱崎 雅成, 徐 亮, 岩崎 倫政 日本整形外科学会雑誌 91 (8) S1467 -S1467 2017年08月 [査読無し][通常論文]
  
• 関節軟骨の再生医療 マウスiPS細胞の軟骨分化誘導と糖鎖解析を用いた新規バイオマーカーの探索

  本谷 和俊, 小野寺 智洋, 寺島 理代, 門間 太輔, 松岡 正剛, 上徳 善太, 松原 新史, 宝満 健太郎, 菱村 亮介, 濱崎 雅成, 金 佑泳, 徐 亮, 田 園, 岩崎 倫政 日本整形外科学会雑誌 91 (8) S1469 -S1469 2017年08月 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術における内側側副靱帯浅層剥離の影響 定量的膝外反ストレス撮影による検討

  佐藤 大, 薮内 康史, 小野寺 智洋, 亀田 敏明, 岩崎 倫政, 近藤 英司, 小野寺 純, 北村 信人, 安田 和則, 八木 知徳 北海道整形災害外科学会雑誌 59 (1) 162 -163 2017年08月 [査読無し][通常論文]
  
• 陳旧性膝複合靱帯損傷に対する後外側支持機構再建術(Clancy変法)の治療成績

  松原 新史, 近藤 英司, 小野寺 純, 小野寺 智洋, 亀田 敏明, 北村 信人, 安田 和則, 岩崎 倫政 日本整形外科スポーツ医学会雑誌 37 (4) 374 -374 2017年08月 [査読無し][通常論文]
  
• 膝屈筋腱ハイブリッド材料を用いた解剖学的2束後十字靱帯再建術の臨床成績

  亀田 敏明, 近藤 英司, 小野寺 智洋, 後藤 圭子, 小野寺 純, 北村 信人, 川村 大介, 安田 和則, 岩崎 倫政 日本整形外科スポーツ医学会雑誌 37 (4) 396 -396 2017年08月 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術後のスポーツおよび社会復帰に関する検討

  薮内 康史, 近藤 英司, 小野寺 純, 亀田 敏明, 佐藤 大, 小野寺 智洋, 放生 憲博, 八木 知徳, 安田 和則, 岩崎 倫政 日本整形外科スポーツ医学会雑誌 37 (4) 533 -533 2017年08月 [査読無し][通常論文]
  
• 膝屈筋腱ハイブリッド代用材料を用いた解剖学的2束前・後十字靱帯同時再建術の2例

  珍部 正嗣, 近藤 英司, 小野寺 純, 石田 博英, 小野寺 智洋, 岩崎 浩司, 下出 和美, 安田 和則, 岩崎 倫政 日本整形外科スポーツ医学会雑誌 37 (4) 607 -607 2017年08月 [査読無し][通常論文]
  
• 鏡視下膝靱帯再建術後における急性関節内感染 同一施設における10年間の検討

  亀田 敏明, 小野寺 智洋, 藪内 康史, 岩崎 倫政, 近藤 英司, 小野寺 純, 北村 信人, 安田 和則 北海道整形災害外科学会雑誌 59 (1) 118 -119 2017年08月 [査読無し][通常論文]
  
• TriS Medial HTO Plate Systemを用いた内側楔状開大式高位脛骨骨切り術

  薮内 康史, 小野寺 智洋, 亀田 敏明, 佐藤 大, 岩崎 倫政, 近藤 英司, 小野寺 純, 北村 信人, 安田 和則, 八木 知徳 北海道整形災害外科学会雑誌 59 (1) 162 -162 2017年08月 [査読無し][通常論文]
  
• 膝関節に発生したびまん型色素性絨毛結節性滑膜炎の治療成績

  新井 隆太, 小野寺 智洋, 薮内 康史, 亀田 敏明, 高橋 大介, 入江 徹, 浅野 毅, 下段 俊, 岩崎 倫政, 近藤 英司, 安田 和則 北海道整形災害外科学会雑誌 59 (1) 168 -169 2017年08月 [査読無し][通常論文]
  
• Taylor Spatial Frameを用いて治療した脛腓骨遠位部骨折後変形治癒の一例

  小池 良直, 小野寺 智洋, 藪内 康史, 亀田 敏明, 新井 隆太, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 59 (1) 173 -173 2017年08月 [査読無し][通常論文]
  
• 関節軟骨の再生医療 高純度硬化性アルギン酸ゲル移植は家兎半月板部分欠損モデルにおいて半月板修復を促進する

  金 佑泳, 近藤 英司, 小野寺 智洋, 野々山 貴行, 馬場 力哉, 本谷 和俊, 上徳 善太, 松原 新史, 宝満 健太郎, 菱村 亮介, Terkawi Muhamad, 岩崎 倫政 日本整形外科学会雑誌 91 (8) S1468 -S1468 2017年08月 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術後の膝蓋大腿関節における応力変化 CT osteoabsorptiometry法を用いて

  亀田 敏明, 近藤 英司, 薮内 康史, 小野寺 智洋, 小野寺 純, 八木 知徳, 安田 和則, 岩崎 倫政 日本整形外科学会雑誌 91 (8) S1694 -S1694 2017年08月 [査読無し][通常論文]
  
• 高位脛骨骨切り術における深部静脈血栓症の検討

  小池 良直, 亀田 敏明, 近藤 英司, 藪内 康史, 下段 俊, 浅野 毅, 新井 隆太, 入江 徹, 髙橋 大介, 小野寺 智洋, 安田 和則, 岩崎 倫政 北海道整形災害外科学会雑誌 : 北海道整形災害外科学会機関誌 59 (1) 78 -84 2017年08月 [査読無し][通常論文]
  
• 学術奨励賞・受賞記念論文 Syndecan-4 regulates development of autoimmune arthritis by modulating B cell migration and germinal center formation : 自己免疫性関節炎におけるシンデカン-4の機能解析

  遠藤 努, 伊藤 甲雄, 森本 純子, 金山 剛士, 太田 大地, 池末 昌弘, 今 重之, 小野寺 智洋, 高橋 大介, 岩崎 倫政, 上出 利光 北海道整形災害外科学会雑誌 : 北海道整形災害外科学会機関誌 59 (1) 11 -17 2017年08月 [査読無し][通常論文]
   

  関節リウマチ(RA)は代表的な自己免疫性疾患の一つである。いくつかの臨床研究においてB細胞標的治療がRAに有効であることが実証され、さらにRA患者の炎症滑膜にはリンパ濾胞様構造が観察されることから、リンパ濾胞・胚中心形成、及びB細胞による自己抗体産生がRAの病態にとって重要であることが示唆されている。本研究ではコラーゲン誘導性関節炎;CIAモデルを用いて、B細胞におけるSyndecan-4(Syn4)の機能を明らかにすることを目的とした。CIAモデルにおいてSyn4は病態を悪化させ、所属リンパ節の胚中心形成と自己抗体産生を促すことが明らかとなった。またSyn4は胚中心形成過程のB細胞遊走に重要なSDF-1に対する遊走能を促進させ、in vivoにおいて所属リンパ節へのB細胞遊走を促進させた。本研究によって、自己免疫性関節炎におけるSyn4の機能がはじめて明らかとなった。(著者抄録)
• 膝軟骨欠損に対する自家培養軟骨細胞移植(JACC)の短期成績

  甲斐原 拓真, 薮内 康史, 小野寺 智洋, 亀田 敏明, 岩崎 倫政, 近藤 英司, 遠山 晴一, 安田 和則 北海道整形災害外科学会雑誌 59 (1) 166 -166 2017年08月 [査読無し][通常論文]
  
• 超高分子ポリエチレン摩耗粉によるヒトマクロファージの遺伝子発現変化は関節リウマチと類似する

  Terkawi Alaa, 濱崎 雅成, 高橋 大介, 角家 健, 浅野 毅, 入江 徹, 小野寺 智洋, 高畑 雅彦, 岩崎 倫政 日本整形外科学会雑誌 91 (8) S1722 -S1722 2017年08月 [査読無し][通常論文]
  
• 自家骨軟骨柱移植術に高純度アルギン酸ゲルを併用した治療効果の検証

  菱村 亮介, 小野寺 智洋, 馬場 力哉, 本谷 和俊, 宝満 健太郎, 松原 新史, 上徳 善太, 金 佑泳, 岩崎 倫政 日本整形外科学会雑誌 91 (8) S1727 -S1727 2017年08月 [査読無し][通常論文]
  
• 軟骨細胞にはNF-κB arthritis inducer 1を介する炎症回路活性化機構が存在する

  太田 光俊, 田中 勇希, 蒋 菁菁, 中川 育磨, 樋口 はるか, 藤田 宗純, 有馬 康伸, 熱海 徹, 上村 大輔, 小野寺 智洋, 村上 正晃, 岩崎 倫政 日本整形外科学会雑誌 91 (8) S1728 -S1728 2017年08月 [査読無し][通常論文]
  
• 新規ロッキングプレートを用いた内側楔状開大式高位脛骨骨切り術の生体力学的および臨床的評価 従来のプレートとの比較

  薮内 康史, 近藤 英司, 小野寺 純, 亀田 敏明, 佐藤 大, 小野寺 智洋, 放生 憲博, 八木 知徳, 岩崎 倫政, 安田 和則 日本整形外科学会雑誌 91 (8) S1765 -S1765 2017年08月 [査読無し][通常論文]
  
• 軟骨細胞の力学的ストレス応答におけるスフィンゴ糖脂質の機能解析

  松原 新史, 小野寺 智洋, 前田 英次郎, 門間 太輔, 馬場 力哉, 本谷 俊彦, 上徳 善太, 宝満 健太郎, 大橋 俊朗, 岩崎 倫政 日本整形外科学会雑誌 91 (8) S1774 -S1774 2017年08月 [査読無し][通常論文]
  
• 膝複合靱帯損傷を合併した脛骨高原骨折術後下肢外反変形に対してDouble Level Osteotomyを行った1例

  下田 康平, 珍部 正嗣, 近藤 英司, 小野寺 智洋, 新井 隆太, 岩崎 浩司, 小林 英之, 鈴木 瞭太, 忠鉢 敏弥, 岩崎 倫政 東日本整形災害外科学会雑誌 29 (3) 286 -286 2017年08月 [査読無し][通常論文]
  
• Tris Medial HTO Plate Systemを用いた内側楔状開大式高位脛骨骨切り術の短期成績

  薮内 康史, 近藤 英司, 小野寺 純, 亀田 敏明, 小野寺 智洋, 佐藤 大, 八木 知徳, 安田 和則, 岩崎 倫政 東日本整形災害外科学会雑誌 29 (3) 392 -392 2017年08月 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術における内側側副靱帯浅層剥離の影響 定量的膝外反ストレス撮影による検討

  佐藤 大, 薮内 康史, 小野寺 智洋, 亀田 敏明, 岩崎 倫政, 近藤 英司, 小野寺 純, 北村 信人, 八木 知徳, 坂本 圭太, 高澤 啓, 安田 和則 日本関節病学会誌 36 (2) 131 -139 2017年07月 [査読無し][通常論文]
   

  内側側副靱帯(MCL)浅層の脛骨付着部を剥離して行った内側楔状開大式高位脛骨骨切り術(OWHTO)術後の外反不安定性を検討した。対象は、2009年から2015年にOWHTOを行った84人93膝(男性19人20膝、女性65人73膝、手術時平均60.1歳)とした。疾患は内側変形性膝関節症(OA)74例83膝、突発性膝骨壊死(ON)10例10膝であった。JOAスコアは術前平均69.1点から術後90.3点に有意に改善した。定量的外反ストレス撮影において、内側関節裂隙の開大距離(MJS)は術前から全身麻酔下のMCL浅層剥離直前で有意に増加し、MCL浅層の剥離直後にはさらに有意に増加したが、術後1年以降では術前との間に有意差は認めなかった。術後の開大距離と術中、術後のMJSと術後の開大角と術中、術後のJLCAについての相関関係を検討した結果、いずれの項目についても有意な相関係数を認めなかった。Locking plate抜去部は線維性瘢痕組織で覆われていた。Spot MRIでは、iso-intensityの肥厚したMCL浅層線維を認めた。組織所見では、OWHTO術後に骨とMCL浅層の間に瘢痕組織が介在し、不規則な膠原線維の配列を呈した。以上より、locking plateを用いたOWHTO後の短期成績は良好であった。全症例で、MCL浅層の脛骨付着部を完全に剥離してOWHTOを行ったが、MCL浅層剥離による術後の膝外反不安定性は認めなかった。
• Tris Medial HTO Plate Systemを用いた内側楔状開大式高位脛骨骨切り術の短期成績 抜釘症例における従来のプレートとの比較

  薮内 康史, 近藤 英司, 小野寺 純, 亀田 敏明, 佐藤 大, 小野寺 智洋, 八木 知徳, 岩崎 倫政, 安田 和則 JOSKAS 42 (4) 527 -527 2017年05月 [査読無し][通常論文]
  
• 陳旧性膝重度複合靱帯損傷に対する一期的再建術の治療成績

  松原 新史, 近藤 英司, 小野寺 純, 小野寺 智洋, 亀田 敏明, 北村 信人, 安田 和則, 岩崎 倫政 JOSKAS 42 (4) 617 -617 2017年05月 [査読無し][通常論文]
  
• 膝軟骨欠損に対するアテロコラーゲンを用いた自家培養軟骨移植の短〜中期成績

  甲斐原 拓真, 近藤 英司, 亀田 敏明, 小野寺 智洋, 薮内 康史, 遠山 晴一, 安田 和則, 岩崎 倫政 JOSKAS 42 (4) 285 -285 2017年05月 [査読無し][通常論文]
  
• 高度内反変形および膝蓋骨亜脱臼を伴う変形性膝関節症に対して逆V字型高位脛骨骨切り術およびBlauth‐Mann変法を行った一例

  松居祐樹, 亀田敏明, 小野寺智洋, 寺尾英将, 加藤琢磨, 林晴久, 岩崎倫政, 近藤英司, 小野寺純, 北村信人, 安田和則 北海道整形災害外科学会雑誌 58 (2) 336‐337 2017年03月24日 [査読無し][通常論文]
  
• 高マンノース型糖鎖の減少は可逆的軟骨変性を引き起こす

  宝満健太郎, 小野寺智洋, 古川潤一, 門間太輔, 松岡正剛, 馬場力哉, 本谷和俊, 上徳善太, 松原新史, 菱村亮介, KIM Wooyoung, 濱崎雅成, XU Liang, TIAN Yuan, 岩崎倫政 日本軟骨代謝学会プログラム・抄録集 30th 112 2017年03月 [査読無し][通常論文]
  
• 軟骨細胞の力学的ストレス応答におけるスフィンゴ糖脂質の機能解析

  松原新史, 小野寺智洋, 前田英次郎, 門間太輔, 馬場力哉, 本谷和俊, 上徳善太, 宝満健太郎, 大橋俊朗, 岩崎倫政 第30回日本軟骨代謝学会 120 2017年03月 [査読無し][通常論文]
  
• 高純度アルギン酸ゲルを併用した骨髄刺激法の効果―ビーグル犬骨軟骨欠損モデルを用いて―

  馬場力哉, 小野寺智洋, 松岡正剛, 本谷和俊, 上徳善太, 松原新史, 宝満健太郎, 岩崎倫政 第30回日本軟骨代謝学会 66 2017年03月 [査読無し][通常論文]
  
• 軟骨自然治癒過程に発現するCCL21による軟骨修復の検討

  上徳善太, 小野寺智洋, 古川潤一, 門間太輔, 松岡正剛, 馬場力哉, 本谷和俊, 松原新史, 宝満健太郎, 菱村亮介, 岩崎倫政 第30回日本軟骨代謝学会 30th 94 2017年03月 [査読無し][通常論文]
  
• 高マンノース型糖鎖の減少は可逆的軟骨変性を引き起こす

  宝満健太郎, 小野寺智洋, 古川潤一, 門間太輔, 松岡正剛, 馬場力哉, 本谷和俊, 上徳善太, 松原新史, 菱村亮介, 金佑泳, 濱崎雅成, 徐亮,田園, 岩崎倫政 第30回日本軟骨代謝学会 30th 120 2017年03月 [査読無し][通常論文]
  
• 糖鎖解析を用いたiPS細胞からの軟骨分化における新規バイオマーカーの探索

  本谷和俊, 小野寺智洋, 寺島理代, 門間太輔, 松岡正剛, 馬場力哉, 上徳善太, 松原新史, 宝満健太郎, 菱村亮介, 金佑泳, 濱崎雅成, 徐亮,田園, 岩崎倫政 第30回日本軟骨代謝学会 30th 101 2017年03月 [査読無し][通常論文]
  
• Identification Of Differentiation Biomarkers For The Chondrogenic Differentiation Of Induced Pluripotent Stem Cells.

  Kazutoshi Hontani, Tomohiro Onodera, Daisuke Momma, Masatake Matsuoka, Rikiya Baba, Zenta Joutoku, Shinji Matsubara, Kentaro Homan, Kentaro Homan, Ryosuke Hishimura, Kim WooYoung, Masanari Hamasaki, Liang Xu, Yuan Tian, Norimasa Iwasaki The 63th Annual Meeting of Orthopaedic Research Society (ORS) 2017年03月 [査読無し][通常論文]
  
• Depletion Of Gangliosides Enhances Articular Cartilage Repair By Suppressing The Chondrocyte Hypertrophy.

  Masatake Matsuoka, Tomohiro Onodera, Kentaro Homan, Fumio Sasazawa, Daisuke Momma, Rikiya Baba, Kazutoshi Hontani, Zenta Joutoku, Shinji Matsubara, Ryosuke Hishimura, Norimasa Iwasaki The 63th Annual Meeting of Orthopaedic Research Society (ORS) 2017年03月 [査読無し][通常論文]
  
• Effects Of Autologous Osteochondral Transplantation Augmented By Ultrapurified Alginate Gel In Rabbit Model.

  Ryosuke Hishimura, Tomohiro Onodera, Kazutoshi Hontani, Rikiya Baba, Kentaro Homan, Shinji Matsubara, Zenta Joutoku, WooYoung Kim, Norimasa Iwasaki The 63th Annual Meeting of Orthopaedic Research Society (ORS) 2017年03月 [査読無し][通常論文]
  
• CCL21 Expressed in Cartilage Repair Process in Immature Mice and Accelerated Cartilage Repair.

  Zenta Joutoku, Tomohiro Onodera, Daisuke Momma, Masatake Matsuoka, Rikiya Baba, Kazutoshi Hontani, Shinji Matsubara, Kentaro Homan, Ryosuke Hishimura, Norimasa Iwasaki The 63th Annual Meeting of Orthopaedic Research Society (ORS) 2017年03月 [査読無し][通常論文]
  
• The enzymatic cleavage of high-mannose type N-glycan induces recoverable cartilage degradation.

  Kentaro Homan, Tomohiro Onodera, Jun-ichi Furukawa, Daisuke Momma, Masatake Matsuoka, Rikiya Baba, Kazutoshi Hontani, Zenta Joutoku, Shinji Matsubara, Ryosuke Hishimura, Kim WooYoung, Masanari Hamasaki, Liang Xu, Norimasa Iwasaki The 63th Annual Meeting of Orthopaedic Research Society (ORS) 2017年03月 [査読無し][通常論文]
  
• 三次元培養下における伸張ストレス負荷モデルの確立

  松原 新史, 小野寺 智洋, 門間 太輔, 松岡 正剛, 馬場 力哉, 本谷 和俊, 上徳 善太, 宝満 健太郎, キム・ウヨン, 岩崎 倫政 北海道整形災害外科学会雑誌 58 (2) 270 -271 2017年03月 [査読無し][通常論文]
  
• ガングリオシド欠損はIndian Hedgehog pathwayを介し軟骨の肥大化を抑制する

  松岡 正剛, 小野寺 智洋, 笹沢 史生, 門間 太輔, 馬場 力哉, 本谷 和俊, 上徳 善太, 宝満 健太郎, 岩崎 倫政 北海道整形災害外科学会雑誌 58 (2) 269 -270 2017年03月 [査読無し][通常論文]
  
• 新しいLocking plate(Tris Medial HTO Plate System)を用いた内側楔状開大式高位脛骨骨切り術

  薮内 康史, 小野寺 智洋, 亀田 敏明, 岩崎 倫政, 類家 拓也, 伊藤 浩, 近藤 英司, 小野寺 純, 北村 信人, 安田 和則, 八木 知徳 北海道整形災害外科学会雑誌 58 (2) 268 -268 2017年03月 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術後の膝蓋大腿関節における応力変化 CT osteoabsorptiometry法を用いて

  亀田 敏明, 小野寺 智洋, 薮内 康史, 岩崎 倫政, 近藤 英司, 小野寺 純, 北村 信人, 安田 和則, 八木 知徳 北海道整形災害外科学会雑誌 58 (2) 268 -269 2017年03月 [査読無し][通常論文]
  
• ハイブリッド型人工股関節の術後中・長期成績

  入江 徹, 高橋 大介, 浅野 毅, 下段 俊, 亀田 敏明, 新井 隆太, 小野寺 智洋, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 58 (2) 277 -278 2017年03月 [査読無し][通常論文]
  
• THA術後の骨代謝状態の変化の検討

  浅野 毅, 高橋 大介, 入江 徹, 下段 俊, 新井 隆太, 亀田 敏明, 薮内 康史, 小野寺 智洋, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 58 (2) 279 -280 2017年03月 [査読無し][通常論文]
  
• 大腿骨頭軟骨下脆弱性骨折の予後予測因子の検討 免荷保存治療は効果があるのか

  高橋 大介, 入江 徹, 浅野 毅, 下段 俊, 新井 隆太, 薮内 康史, 亀田 敏明, 小野寺 智洋, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 58 (2) 280 -280 2017年03月 [査読無し][通常論文]
  
• 経過中にMRI所見が変化した大腿骨頭軟骨下脆弱性骨折の1例

  細川 吉暁, 浅野 毅, 高橋 大介, 入江 徹, 新井 隆太, 下段 俊, 亀田 敏明, 薮内 康史, 小野寺 智洋, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 58 (2) 280 -281 2017年03月 [査読無し][通常論文]
  
• 大腿骨転子間彎曲内反骨切り術が股関節外転角度に与える影響

  下段 俊, 入江 徹, 高橋 大介, 浅野 毅, 新井 隆太, 亀田 敏明, 小野寺 智洋, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 58 (2) 290 -290 2017年03月 [査読無し][通常論文]
  
• 非定型大腿骨骨折19例の検討 骨折部位別の比較検討

  吉田 朋世, 高橋 大介, 入江 徹, 浅野 毅, 小野寺 智洋, 岩崎 倫政, 梅本 貴央, 田辺 明彦, 宮崎 拓自, 山本 励志, 近藤 英司 北海道整形災害外科学会雑誌 58 (2) 293 -293 2017年03月 [査読無し][通常論文]
  
• 高位脛骨骨切り術における深部静脈血栓症の検討

  小池 良直, 亀田 敏明, 藪内 康史, 下段 俊, 浅野 毅, 新井 隆太, 入江 徹, 高橋 大介, 小野寺 智洋, 岩崎 倫政, 近藤 英司, 安田 和則 北海道整形災害外科学会雑誌 58 (2) 307 -308 2017年03月 [査読無し][通常論文]
  
• 距骨離断性骨軟骨炎に対して経内果的鏡視下アプローチにて骨軟骨柱移植を施行した一例

  小野寺 智洋, 近藤 英司, 岩崎 倫政, 内田 淳 北海道整形災害外科学会雑誌 58 (2) 312 -313 2017年03月 [査読無し][通常論文]
  
• 外反母趾変形矯正術前後における第1遠位指節間関節外反変形の検討

  上徳 善太, 小野寺 智洋, 松岡 正剛, 近藤 英司, 亀田 敏明, 薮内 康史, 岩崎 倫政, 山崎 修司 北海道整形災害外科学会雑誌 58 (2) 314 -314 2017年03月 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術における内側側副靱帯浅層剥離の影響 定量的膝外反ストレス撮影による検討

  佐藤 大, 近藤 英司, 薮内 康史, 小野寺 純, 小野寺 智洋, 亀田 敏明, 八木 知徳, 岩崎 倫政, 安田 和則 日本整形外科学会雑誌 91 (2) S32 -S32 2017年03月 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術後の膝蓋大腿関節における応力変化 CT osteoabsorptiometry法を用いて

  亀田 敏明, 近藤 英司, 薮内 康史, 小野寺 智洋, 小野寺 純, 安田 和則, 岩崎 倫政 日本整形外科学会雑誌 91 (2) S33 -S33 2017年03月 [査読無し][通常論文]
  
• 鏡視下足関節固定術における距腿関節間距離および足関節固定角度と骨癒合期間との関係

  林 晴久, 小野寺 智洋, 近藤 英司, 亀田 敏明, 加藤 琢磨, 岩崎 倫政 日本整形外科学会雑誌 91 (2) S87 -S87 2017年03月 [査読無し][通常論文]
  
• 鏡視下膝靱帯再建術後における急性関節内感染 同一施設における15年間の検討

  亀田 敏明, 近藤 英司, 薮内 康史, 小野寺 智洋, 小野寺 純, 北村 信人, 安田 和則, 岩崎 倫政 日本整形外科学会雑誌 91 (2) S245 -S245 2017年03月 [査読無し][通常論文]
  
• 膝軟骨欠損に対するアテロコラーゲンを用いた自家培養軟骨移植の短〜中期成績

  甲斐原 拓真, 亀田 敏明, 近藤 英司, 小野寺 智洋, 薮内 康史, 遠山 晴一, 安田 和則, 岩崎 倫政 日本整形外科学会雑誌 91 (3) S1142 -S1142 2017年03月 [査読無し][通常論文]
  
• 自家骨軟骨柱移植術に高純度アルギン酸ゲルをaugumentationした治療効果の検討

  菱村亮介, 小野寺智洋, 馬場力也, 本谷和俊, 宝満健太郎, 松原新史, 上徳善太, 金佑泳, 岩崎倫政 北海道整形災害外科学会 132nd 5 2017年 [査読無し][通常論文]
  
• 高度内反変形と膝蓋骨亜脱臼を伴う両変形性膝関節症に対して逆V字型高位脛骨骨切り術およびBlauth‐Mann変法を行った一例

  松居祐樹, 亀田敏明, 小野寺智洋, 寺尾英将, 加藤琢磨, 林晴久, 岩崎倫政, 近藤英司, 川村澄人, 小野寺純, 北村信人, 安田和則 北海道整形災害外科学会 132nd 84 2017年 [査読無し][通常論文]
  
• Chevron osteotomyを用いて行った距骨骨軟骨損傷の短期成績

  小野寺純, 北村信人, 近藤英司, 小野寺智洋, 岩崎倫政, 上田大輔, 八木知徳, 安田和則 北海道整形災害外科学会 132nd 38 2017年 [査読無し][通常論文]
  
• 膝屈筋腱ハイブリッド代用材料を用いた解剖学的2束前十字靱帯再建術の臨床成績:術前待機期間の影響

  馬場力哉, 近藤英司, 小野寺智洋, 亀田敏明, 岩崎倫政, 小野寺純, 北村信人, 安田和則 北海道整形災害外科学会 132nd 89 2017年 [査読無し][通常論文]
  
• 遺残組織を温存した解剖学的2束前十字靱帯再建術後のCyclops病変:非温存群との比較

  林晴久, 小野寺智洋, 亀田敏明, 加藤琢磨, 岩崎倫政, 近藤英司, 小野寺純, 北村信人, 安田和則 北海道整形災害外科学会 132nd 89 2017年 [査読無し][通常論文]
  
• 広範な大腿骨内側顆軟骨損傷に対して骨軟骨柱移植および高位脛骨骨切り術を行った一例

  五月女慧人, 亀田敏明, 小野寺智洋, 藤田諒, 加藤琢磨, 林晴久, 岩崎倫政, 近藤英司, 小野寺純, 北村信人, 安田和則, 福井孝明 北海道整形災害外科学会 132nd 83 2017年 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術における内側側副靱帯浅層剥離の影響 : 定量的膝外反ストレス撮影による検討

  佐藤 大, 坂本 圭太, 高澤 啓, 安田 和則, 薮内 康史, 小野寺 智洋, 亀田 敏明, 岩崎 倫政, 近藤 英司, 小野寺 純, 北村 信人, 八木 知徳 日関病誌 36 (2) 131 -139 2017年 [査読無し][通常論文]
   

  <p><b>Background:</b> To perform a medial open-wedge high tibial osteotomy (OWHTO), surgeons frequently expose the medial-proximal tibia by partial or complete distal release of the overlying insertion of the superficial layer of the medial collateral ligament (sMCL). Biomechanically, the sMCL is the primary restraint to valgus forces. Therefore, any release of the sMCL can lead to valgus instability of the knee joint. The purpose of this study was to clarify the valgus instability after the release of the distal attachment of the sMCL in OWHTO.</p><p><b>Methods:</b> Between 2009 and 2015, 84 consecutive patients (93 knees) who underwent OWHTO using a locking plate were enrolled in this study. There were 19 men and 65 women with a mean age of 60 years (range; 20 to 78 years) at the time of surgery. We performed the complete release of the distal attachment of the sMCL. Then, a biplanar osteotomy of the tibia was performed. A beta-TCP spacer was implanted in the opening space. Then, a locking plate was implanted onto the tibia. All patients underwent clinical and radiological examinations preoperatively and at one year postoperatively. To assess objective medial instability, joint line convergence angle (JLCA) and the medial joint space (MJS) were evaluated.</p><p><b>Results:</b> Postoperatively, the mean Japan Orthopaedic Association score significantly improved from 69 to 90 points (total score: 100 points, <i>P</i><0.0001). The JLCA and MJS significantly increased immediately after the release of the distal attachment of the sMCL intraoperatively (6.7° and 11.4 mm, <i>P</i><0.0001). However, there were no significant differences in the JLCA and MJS between preoperative (0.4° and 6.7 mm) and one-year postoperative evaluations (0.4° and 6.3 mm).</p><p><b>Conclusion:</b> The complete release of the sMCL did not cause postoperative valgus instability at one year after OWHTO procedure.</p>
• 豚丹毒生ワクチン針刺し後に右膝慢性滑膜炎をきたした1例

  渡辺 尭仁, 小野寺 智洋, 新井 隆太, 亀田 敏明, 薮内 康史, 近藤 英司, 岩崎 倫政, 迫田 義博 北海道医学雑誌 91 (2) 88 -89 2016年11月 [査読無し][通常論文]
  
• 豚丹毒生ワクチン針刺し後に右膝慢性滑膜炎をきたした1例

  渡辺 尭仁, 小野寺 智洋, 新井 隆太, 亀田 敏明, 薮内 康史, 近藤 英司, 岩崎 倫政, 迫田 義博 北海道医学雑誌 91 (2) 88 -89 2016年11月 [査読無し][通常論文]
  
• 遺残組織を温存した解剖学的2束前十字靱帯再建術後のCyclops病変:非温存群との比較

  林晴久, 近藤英司, 小野寺純, 北村信人, 小野寺智洋, 亀田敏明, 加藤琢磨, 安田和則, 岩崎倫政 日本関節病学会誌 35 (3) 289 2016年10月14日 [査読無し][通常論文]
  
• 学術奨励賞・受賞記念論文 iPS細胞とES細胞の神経分化過程における糖鎖構造の比較

  寺島 理代, 小野寺 智洋, 岩崎 倫政 北海道整形災害外科学会雑誌 : 北海道整形災害外科学会機関誌 58 (1) 1 -8 2016年10月
• 高純度アルギン酸gelを併用した骨髄刺激法の効果 ーbeagle犬骨軟骨欠損モデルを用いてー

  馬場力哉, 小野寺智洋, 本谷和俊, 上徳善太, 松原新史, 宝満健太郎, 岩崎倫政 日本バイオマテリアル学会シンポジウム2016 2016 25 2016年10月 [査読無し][通常論文]
  
• ultra-purified alginate gel (UPAL gel) を用いたマウスiPS細胞からの軟骨分化誘導

  本谷和俊, 小野寺智洋, 寺島理代, 門間太輔, 松岡正剛, 馬場力哉, 上徳善太, 松原新史, 宝満健太郎, 菱村亮介, 金佑泳, 徐亮, 岩崎倫政 日本バイオマテリアル学会シンポジウム 2016 13 2016年10月 [査読無し][通常論文]
  
• 骨軟骨再生に特異的に作用するケモカインの網羅的解析

  上徳善太, 小野寺智洋, 門間太輔, 松岡正剛, 馬場力哉, 本谷和俊, 松原新史, 宝満健太郎, 岩崎倫政 北海道整形災害外科学会雑誌 58 (1) 82 -83 2016年10月 [査読無し][通常論文]
  
• Ultra-purified alginate gel(UPAL gel)を用いたiPS細胞からの軟骨誘導

  本谷 和俊, 小野寺 智洋, 寺島 理代, 門間 太輔, 松岡 正剛, 馬場 力哉, 上徳 善太, 松原 新史, 宝満 健太郎 北海道整形災害外科学会雑誌 58 (1) 83 -84 2016年10月 [査読無し][通常論文]
  
• 高マンノース型糖鎖構造変化に伴う軟骨変性へのアプローチ

  宝満 健太郎, 小野寺 智洋, 門間 太輔, 松岡 正剛, 馬場 力哉, 本谷 和俊, 松原 新史, 上徳 善太, 岩崎 倫政 北海道整形災害外科学会雑誌 58 (1) 83 -83 2016年10月 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術後のスポーツ復帰に関する検討

  薮内 康史, 小野寺 智洋, 亀田 敏明, 岩崎 倫政, 小野寺 純, 横田 正司, 北村 信人, 安田 和則, 八木 知徳, 近藤 英司 北海道整形災害外科学会雑誌 58 (1) 93 -94 2016年10月 [査読無し][通常論文]
   

  <p><b>Objective</b>: The purpose of this study was to evaluate sporting activity and clinical outcome following medial open-wedge high tibial osteotomy (OWHTO) in middle and older-aged patients with medial osteoarthritis (OA).</p><p><b>Methods</b>: Between 2009 and 2014, 22 patients (25 knees) who underwent OWHTO with a locking plate were enrolled in this study. All patients were active in sports on a regular basis prior to surgery, but were unable to perform sports at the time of surgery. Inclusion criteria involved patients who had medial OA or spontaneous osteonecrosis of the knee. There were 10 men and 12 women with a mean age of 60.3 years (range: 41-74 years) at the time of surgery. We performed a biplanar osteotomy of the tibia intraoperatively. A beta-TCP spacer was implanted in the opening space. Then, a locking plate was implanted onto the tibia. All patients underwent sporting activities, clinical and radiological examinations, with determinations of complications prior to surgery and at the final follow up periods after surgery (mean 37.2 months, range: 13.0-73.1 months).</p><p><b>Results</b>: Postoperatively, the mean Japan Orthopaedic Association score significantly improved from 68.2 to 92.6 points (Total score: 100 points, <i>P</i><0.001). The Tegner activity level scale did not show significant changes pre- and postoperatively (4.1 to 3.8). However, three patients who engaged in high impact sports (competitive skiing and marathon) had decreased Tegner activity level scale after surgery.</p><p><b>Conclusion</b>: The present study has demonstrated that OWHTO for the treatment of OA in middle and older-aged patients significantly improved the short-term clinical outcome and allowed patients to return to sporting activities similar to their preoperative levels.</p>
• 内側楔状開大式高位脛骨骨切り術における内側側副靱帯浅層剥離の影響 定量的膝外反ストレス撮影による検討

  佐藤 大, 近藤 英司, 薮内 康史, 小野寺 純, 小野寺 智洋, 亀田 敏明, 八木 知徳, 岩崎 倫政, 安田 和則 日本関節病学会誌 35 (3) 252 -252 2016年10月 [査読無し][通常論文]
  
• 当科におけるリウマチ性前足部変形に対する第1MTP関節温存術の治療成績

  小野寺 智洋, 林 晴久, 亀田 敏明, 加藤 琢磨, 近藤 英司, 新井 隆太, 岩崎 倫政 日本足の外科学会雑誌 37 (2) S180 -S180 2016年10月 [査読無し][通常論文]
  
• 鏡視下足関節固定術における術後距腿関節間距離と骨癒合期間との関係

  林 晴久, 小野寺 智洋, 近藤 英司, 新井 隆太, 亀田 敏明, 加藤 琢磨, 岩崎 倫政 日本足の外科学会雑誌 37 (2) S234 -S234 2016年10月 [査読無し][通常論文]
  
• Vancouver B1大腿骨ステム周囲骨折に対するdual plating法の力学的有用性 力学試験およびFEM解析結果から

  高橋 大介, 浅野 毅, 入江 徹, 下段 俊, 亀田 敏明, 薮内 康史, 小野寺 智洋, 岩崎 倫政, 野山 義裕, 近藤 英司 北海道整形災害外科学会雑誌 58 (1) 96 -97 2016年10月 [査読無し][通常論文]
  
• SLE患者における無症候性IONの存在

  浅野 毅, 高橋 大介, 入江 徹, 下段 俊, 新井 隆太, 亀田 敏明, 薮内 康史, 小野寺 智洋, 近藤 英司, 岩崎 倫政, 渡邉 俊之, 渥美 達也 北海道整形災害外科学会雑誌 58 (1) 98 -99 2016年10月 [査読無し][通常論文]
  
• 人工股関節全置換術後深部静脈血栓症発生率のアプローチによる違い

  入江 徹, 高橋 大介, 浅野 毅, 新井 隆太, 亀田 敏明, 下段 俊, 薮内 康史, 小野寺 智洋, 岩崎 倫政, 高橋 要, 安藤 亮, 紺野 拓也, 近藤 英司 北海道整形災害外科学会雑誌 58 (1) 100 -101 2016年10月 [査読無し][通常論文]
  
• THA術前のJHEQ scoreにおける術後改善度予測因子の検証

  下段 俊, 入江 徹, 高橋 大介, 浅野 毅, 新井 隆太, 亀田 敏明, 藪内 康史, 小野寺 智洋, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 58 (1) 101 -101 2016年10月 [査読無し][通常論文]
  
• 8-plateを用いた成長抑制術を行い脚長調整に2年を要した下肢片側肥大症の一例

  甲斐原 拓真, 小野寺 智洋, 近藤 英司, 薮内 康史, 亀田 敏明, 岩崎 倫政 北海道整形災害外科学会雑誌 58 (1) 137 -138 2016年10月 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術後のスポーツ復帰に関する検討

  薮内 康史, 小野寺 智洋, 亀田 敏明, 岩崎 倫政, 小野寺 純, 横田 正司, 北村 信人, 安田 和則, 八木 知徳, 近藤 英司 北海道整形災害外科学会雑誌 58 (1) 93 -94 2016年10月 [査読無し][通常論文]
  
• The establishment of the novel mechanical stress model in three dimensional culture.

  S. Matsubara, T. Onodera, E. Maeda, D. Momma, M. Matsuoka, R. Baba, K. Hontani, Z. Joutoku, K. Homan, T. Ohashi, N. Iwasaki The13th World Congress of International Chemical Reference Substances (ICRS) 2016年09月 [査読無し][通常論文]
  
• The Efficacy of Chondrogenic differentiation with ultra-purified alginate gel (UPAL gel) for mouse iPS cells in vitro and in vivo.

  K. Hontani, T. Onodera, D. Momma, M. Matsuoka, R. Baba, Z. Joutoku, S. Matsubara, K. Homan, R. Hishimura, N. Iwasaki The13th World Congress of International Chemical Reference Substances (ICRS) 2016年09月 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術後の膝蓋大腿関節における応力変化 CT osteoabsorptiometry法を用いて

  亀田 敏明, 近藤 英司, 小野寺 純, 小野寺 智洋, 安田 和則, 岩崎 倫政 中部日本整形外科災害外科学会雑誌 59 (秋季学会) 243 -243 2016年09月 [査読無し][通常論文]
  
• 高位脛骨骨切り術における深部静脈血栓症の検討

  小池 良直, 亀田 敏明, 近藤 英司, 藪内 康史, 小野寺 智洋, 岩崎 倫政 中部日本整形外科災害外科学会雑誌 59 (秋季学会) 244 -244 2016年09月 [査読無し][通常論文]
  
• 鏡視下膝靱帯再建術後における急性関節内感染 同一施設における10年間の検討

  亀田 敏明, 近藤 英司, 小野寺 純, 小野寺 智洋, 安田 和則, 岩崎 倫政 中部日本整形外科災害外科学会雑誌 59 (秋季学会) 254 -254 2016年09月 [査読無し][通常論文]
  
• 定量的膝外反ストレス撮影による膝内側側副靱帯浅層の生体力学的評価 内側楔状開大式高位脛骨骨切り術における検討

  薮内 康史, 佐藤 大, 近藤 英司, 小野寺 純, 小野寺 智洋, 亀田 敏明, 北村 信人, 八木 知徳, 岩崎 倫政, 安田 和則 日本整形外科学会雑誌 90 (8) S1767 -S1767 2016年08月 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術後の膝蓋大腿関節における応力変化 CT osteoabsorptiometry法を用いて

  亀田 敏明, 近藤 英司, 薮内 康史, 小野寺 純, 小野寺 智洋, 北村 信人, 八木 知徳, 安田 和則, 岩崎 倫政 日本整形外科学会雑誌 90 (8) S1826 -S1826 2016年08月 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術における内側側副靱帯浅層剥離の影響 定量的膝外反ストレス撮影による検討

  佐藤 大, 薮内 康史, 近藤 英司, 小野寺 純, 小野寺 智洋, 亀田 敏明, 北村 信人, 八木 知徳, 岩崎 倫政, 安田 和則 JOSKAS 41 (4) 192 -192 2016年07月 [査読無し][通常論文]
  
• 高位脛骨骨切り術後における深部静脈血栓症の検討

  小池 良直, 亀田 敏明, 近藤 英司, 薮内 康史, 小野寺 智洋, 安田 和則, 岩崎 倫政 JOSKAS 41 (4) 239 -239 2016年07月 [査読無し][通常論文]
  
• 自家培養軟骨細胞移植(JACC)の短期成績

  甲斐原 拓真, 薮内 康史, 近藤 英司, 小野寺 智洋, 亀田 敏明, 岩崎 倫政, 安田 和則 JOSKAS 41 (4) 340 -340 2016年07月 [査読無し][通常論文]
  
• 鏡視下膝靱帯再建術後における急性関節内感染 同一施設における10年間の検討

  亀田 敏明, 近藤 英司, 小野寺 純, 小野寺 智洋, 薮内 康史, 北村 信人, 安田 和則, 岩崎 倫政 JOSKAS 41 (4) 531 -531 2016年07月 [査読無し][通常論文]
  
• 北海道における足の外科 北海道大学での足の外科

  小野寺 智洋, 亀田 敏明, 薮内 康史, 近藤 英司, 岩崎 倫政 北海道整形災害外科学会雑誌 57 (2) 216 -219 2016年04月 [査読無し][通常論文]
  
• TKA後の膝関節kinematic patternに影響を与える術前因子の検討

  清藤 直樹, 小野寺 智洋, 笠原 靖彦, 西尾 悠介, 岩崎 倫政, 近藤 英司, 眞島 任史 北海道整形災害外科学会雑誌 57 (2) 318 -319 2016年04月 [査読無し][通常論文]
  
• 特発性膝骨壊死(Spontaneous osteonecrosis of the knee)に対する骨軟骨柱移植術の治療成績

  薮内 康史, 小野寺 智洋, 清藤 直樹, 高橋 大介, 入江 徹, 浅野 毅, 新井 隆太, 中野 宏昭, 深谷 英昭, 岩崎 倫政, 近藤 英司, 眞島 任史, 笠原 靖彦 北海道整形災害外科学会雑誌 57 (2) 325 -326 2016年04月 [査読無し][通常論文]
  
• 著しく増生した滑膜により膝蓋骨脱臼を呈した膝関節滑膜炎の一例

  新井 隆太, 小野寺 智洋, 清藤 直樹, 高橋 大介, 入江 徹, 浅野 毅, 深谷 英昭, 中野 宏昭, 藪内 康史, 岩崎 倫政, 近藤 英司, 安田 和則 北海道整形災害外科学会雑誌 57 (2) 327 -327 2016年04月 [査読無し][通常論文]
  
• 恒久性膝蓋骨脱臼にGreen変法及びBlauth法合併手術を施行した1例

  濱崎 雅成, 清藤 直樹, 小野寺 智洋, 中野 宏昭, 深谷 英昭, 薮内 康史, 岩崎 倫政, 近藤 英司, 眞島 任史 北海道整形災害外科学会雑誌 57 (2) 328 -328 2016年04月 [査読無し][通常論文]
  
• 変形性足関節症とリウマチ性足関節症における鏡視下足関節固定術の臨床成績の比較

  小野寺 智洋, 清藤 直樹, 深谷 英昭, 中野 宏昭, 薮内 康史, 新井 隆太, 藤田 諒, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 57 (2) 329 -330 2016年04月 [査読無し][通常論文]
  
• リウマチ性足関節症に対するIlizarov創外固定器を用いた足関節固定術と鏡視下足関節固定術の比較検討

  深谷 英昭, 小野寺 智洋, 清藤 直樹, 中野 宏昭, 薮内 康史, 新井 隆太, 高橋 大介, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 57 (2) 330 -330 2016年04月 [査読無し][通常論文]
  
• リウマチ性前足部変形に対する切除関節形成術における術後再脱臼因子の検討

  中野 宏昭, 小野寺 智洋, 清藤 直樹, 深谷 英昭, 新井 隆太, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 57 (2) 330 -331 2016年04月 [査読無し][通常論文]
  
• 著明な骨欠損を伴うTHA再置換術後感染性ゆるみに対しTeriparatideを併用して二期的再置換術を施行した1例

  浅野 毅, 高橋 大介, 入江 徹, 中野 宏昭, 新井 隆太, 清藤 直樹, 深谷 英昭, 薮内 康史, 小野寺 智洋, 岩崎 倫政, 安藤 亮, 紺野 拓也, 近藤 英司 北海道整形災害外科学会雑誌 57 (2) 375 -375 2016年04月 [査読無し][通常論文]
  
• 特発性大腿骨頭壊死症と大腿骨軟骨下脆弱性骨折の患者背景の検討

  薮内 康史, 入江 徹, 高橋 大介, 浅野 毅, 新井 隆太, 清藤 直樹, 中野 宏昭, 深谷 英昭, 小野寺 智洋, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 57 (2) 381 -382 2016年04月 [査読無し][通常論文]
  
• 特発性大腿骨頭壊死症の骨頭圧潰進行はMRIで予測可能か?

  入江 徹, 高橋 大介, 浅野 毅, 中野 宏昭, 新井 隆太, 清藤 直樹, 深谷 英昭, 薮内 康史, 小野寺 智洋, 岩崎 倫政, 安藤 亮, 紺野 拓也, 井上 正弘, 近藤 英司 北海道整形災害外科学会雑誌 57 (2) 382 -383 2016年04月 [査読無し][通常論文]
  
• 前十字靱帯損傷に広範な大腿骨外側顆軟骨損傷および外側壁の陥凹骨折を合併した1例

  清藤 直樹, 小野寺 智洋, 岩崎 倫政, 近藤 英司, 三上 将, 安田 和則 北海道整形災害外科学会雑誌 57 (2) 406 -406 2016年04月 [査読無し][通常論文]
  
• Comprehensive Screening of Chemokines Related to Articular Cartilage Repair.

  Zenta Joutoku, Tomohiro Onodera, Daisuke Momma, Masatake Matsuoka, Rikiya Baba, Kazutoshi Hontani, Shinji Matsubara, Kentaro Homan, Ryosuke Hishimura, WooYoung Kim, Norimasa Iwasaki The 62th Annual Meeting of Orthopaedic Research Society (ORS) 2016年03月 [査読無し][通常論文]
  
• Depletion of Gangliosides Accelerated the Articular Cartilage Repair Through Indian Hedgehog Pathway.

  Masatake Matsuoka, Tomohiro Onodera, Fumio Sasazawa, Daisuke Momma, Rikiya Baba, Kazutoshi Hontani, Zenta Joutoku, Shinji Matsubara, Kentaro Homan, Norimasa Iwasaki The 62th Annual Meeting of Orthopaedic Research Society (ORS) 2016年03月 [査読無し][通常論文]
  
• Depletion of High-mannose Type N-glycans Lead to Cartilage Degradation.

  Kentaro Homan, Tomohiro Onodera, Rikiya Baba, Daisuke Momma, Masatake Matsuoka, Kazutoshi Hontani, Shinji Matsubara, Zenta Joutoku, Ryosuke Hishimura, Kim WooYoung, Norimasa Iwasaki The 62th Annual Meeting of Orthopaedic Research Society (ORS) 2016年03月 [査読無し][通常論文]
  
• 大腿骨頭軟骨下脆弱性骨折の予後予測因子の検討 免荷保存治療は効果があるのか

  高橋 大介, 入江 徹, 浅野 毅, 下段 俊, 新井 隆太, 亀田 敏明, 薮内 康史, 小野寺 智洋, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 90 (2) S124 -S124 2016年03月 [査読無し][通常論文]
  
• SLE患者における無症候性IONの存在

  浅野 毅, 高橋 大介, 入江 徹, 下段 俊, 新井 隆太, 薮内 康史, 亀田 敏明, 小野寺 智洋, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 90 (2) S127 -S127 2016年03月 [査読無し][通常論文]
  
• 人工股関節全置換術後における患者立脚型評価と医師主導型評価の各要素の相関性の検討

  下段 俊, 入江 徹, 高橋 大介, 浅野 毅, 安藤 亮, 紺野 拓也, 亀田 敏明, 新井 隆太, 小野寺 智洋, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 90 (3) S765 -S765 2016年03月 [査読無し][通常論文]
  
• 非定型大腿骨骨折19例の検討 骨折部位別の比較検討

  吉田 朋世, 高橋 大介, 入江 徹, 浅野 毅, 梅本 貴央, 田辺 明彦, 宮崎 拓自, 山本 励志, 小野寺 智洋, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 90 (3) S825 -S825 2016年03月 [査読無し][通常論文]
  
• 人工股関節全置換術後深部静脈血栓症の発生率 前側方アプローチと後側方アプローチの比較検討

  入江 徹, 高橋 大介, 浅野 毅, 下段 俊, 安藤 亮, 紺野 拓也, 亀田 敏明, 新井 隆太, 小野寺 智洋, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 90 (3) S932 -S932 2016年03月 [査読無し][通常論文]
  
• リウマチ性前足部変形に対する第2-5中足骨骨頭切除関節形成術における術後再脱臼リスク因子の検討

  小野寺 智洋, 中野 宏昭, 近藤 英司, 亀田 敏明, 薮内 康史, 岩崎 倫政 日本整形外科学会雑誌 90 (3) S943 -S943 2016年03月 [査読無し][通常論文]
  
• Locking plateを用いた内側楔状開大式高位脛骨骨切り術の中期成績と合併症

  薮内 康史, 近藤 英司, 小野寺 純, 小野寺 智洋, 亀田 敏明, 北村 信人, 八木 知徳, 安田 和則, 岩崎 倫政 日本整形外科学会雑誌 90 (3) S960 -S960 2016年03月 [査読無し][通常論文]
  
• ガングリオシド欠損はIndian hedgehog pathwayを介し軟骨の肥大化を抑制する

  松岡正剛, 小野寺智洋, 笹沢史生, 門間太輔, 馬場力哉, 本谷和俊, 上徳善太, 松原新史, 宝満健太郎, 岩崎倫政 日本軟骨代謝学会プログラム・抄録集 29th 136 2016年02月 [査読無し][通常論文]
  
• 軟骨修復に関連するケモカインの網羅的解析

  上徳善太, 小野寺智洋, 門間太輔, 松岡正剛, 馬場力哉, 本谷和俊, 松原新史, 宝満健太郎, 菱村亮介, 岩崎倫政 第29回日本軟骨代謝学会 90 (8) 138 2016年02月 [査読無し][通常論文]
  
• 三次元培養下における伸長ストレス負荷モデルの確立

  松原新史, 小野寺智洋, 門間太輔, 松岡正剛, 馬場力哉, 本谷和俊, 上徳善太, 宝満健太郎, 菱村亮介, キムウヨン, 岩崎倫政 第29回日本軟骨代謝学会 129 2016年02月 [査読無し][通常論文]
  
• Ultra-purified alginate gel (UPAL gel)と多段階分化誘導法を併用したマウスiPS細胞の生体内における軟骨分化誘導

  本谷和俊, 小野寺智洋, 寺島理代, 門間太輔, 松岡正剛, 馬場力哉, 上徳善太, 松原新史, 宝満健太郎, 菱村亮介, 岩崎倫政 第29回日本軟骨代謝学会 29th 114 2016年02月 [査読無し][通常論文]
  
• 軟骨細胞の高マンノース型糖鎖の減少は生体内で軟骨変性を惹起する

  宝満健太郎, 小野寺智洋, 門間太輔, 松岡正剛, 馬場力哉, 本谷和俊, 松原新史, 上徳善太, 菱村亮介, 金佑泳, 岩崎倫政 第29回日本軟骨代謝学会 29th 109 2016年02月 [査読無し][通常論文]
  
• 高純度アルギン酸ゲルを併用した骨髄刺激法による家兎骨軟骨欠損の修復効果-コラーゲンの配向性と力学強度に着目して-

  馬場力哉, 小野寺智洋, 門間太輔, 松岡正剛, 本谷和俊, 遠藤香織, 東藤正浩, 但野茂, 岩崎倫政 日本軟骨代謝学会プログラム・抄録集 29th 2016年
• 大腿骨転子間弯曲内反骨切り術が股関節外転角に与える影響

  下段俊, 入江徹, 高橋大介, 浅野毅, 新井隆太, 亀田敏明, 小野寺智洋, 近藤英司, 岩崎倫政 北海道整形災害外科学会 130th 90 2016年 [査読無し][通常論文]
  
• ハイブリッド型人工股関節の術後10年以上成績

  入江徹, 高橋大介, 浅野毅, 下段俊, 亀田敏明, 安藤亮, 紺野拓也, 新井隆太, 小野寺智洋, 近藤英司, 岩崎倫政 日本人工関節学会プログラム・抄録集 46th 345 2016年 [査読無し][通常論文]
  
• In vivoにおける高純度アルギン酸ゲルと多段階分化誘導法を併用したマウスiPS細胞軟骨分化誘導

  本谷和俊, 小野寺智洋, 寺島理代, 門間太輔, 松岡正剛, 馬場力哉, 上徳善太, 松原新史, 宝満健太郎, 菱村亮介, 岩崎倫政 日本整形外科学会雑誌 90 (8) S1486 -S1486 2016年 [査読無し][通常論文]
  
• 高純度アルギン酸ゲルと多段階分化誘導法を併用したマウスiPS細胞軟骨分化誘導

  本谷和俊, 小野寺智洋, 寺島理代, 門間太輔, 松岡正剛, 馬場力哉, 上徳善太, 松原新史, 宝満健太郎, 菱村亮介, 金佑泳, 岩崎倫政 北海道整形災害外科学会雑誌 58 (suppl-2) 27 2016年 [査読無し][通常論文]
  
• 骨軟骨修復に関連するケモカインの網羅的解析

  上徳善太, 小野寺智洋, 門間太輔, 松岡正剛, 馬場力哉, 本谷和俊, 松原新史, 宝満健太郎, 岩崎倫政 北海道整形災害外科学会雑誌 58 (suppl-2) 26 2016年 [査読無し][通常論文]
  
• 豚丹毒生ワクチン針刺し後に右膝慢性非特異性滑膜炎をきたした1例

  渡辺尭仁, 小野寺智洋, 新井隆太, 迫田義博, 亀田敏明, 薮内康史, 近藤英司, 岩崎倫政 北海道整形災害外科学会 131st 103 2016年 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術後のスポーツ復帰に関する検討

  薮内 康史, 小野寺 智洋, 亀田 敏明, 岩崎 倫政, 近藤 英司, 小野寺 純, 北村 信人, 安田 和則, 八木 知徳 日本関節病学会誌 35 (2) 149 -154 2016年 [査読無し][通常論文]
   

  <p><b>Objective</b>: The purpose of this study was to evaluate sporting activity and clinical outcome following medial open-wedge high tibial osteotomy (OWHTO) in middle and older-aged patients with medial osteoarthritis (OA).</p><p><b>Methods</b>: Between 2009 and 2014, 22 patients (25 knees) who underwent OWHTO with a locking plate were enrolled in this study. All patients were active in sports on a regular basis prior to surgery, but were unable to perform sports at the time of surgery. Inclusion criteria involved patients who had medial OA or spontaneous osteonecrosis of the knee. There were 10 men and 12 women with a mean age of 60.3 years (range: 41-74 years) at the time of surgery. We performed a biplanar osteotomy of the tibia intraoperatively. A beta-TCP spacer was implanted in the opening space. Then, a locking plate was implanted onto the tibia. All patients underwent sporting activities, clinical and radiological examinations, with determinations of complications prior to surgery and at the final follow up periods after surgery (mean 37.2 months, range: 13.0-73.1 months).</p><p><b>Results</b>: Postoperatively, the mean Japan Orthopaedic Association score significantly improved from 68.2 to 92.6 points (Total score: 100 points, <i>P</i><0.001). The Tegner activity level scale did not show significant changes pre- and postoperatively (4.1 to 3.8). However, three patients who engaged in high impact sports (competitive skiing and marathon) had decreased Tegner activity level scale after surgery.</p><p><b>Conclusion</b>: The present study has demonstrated that OWHTO for the treatment of OA in middle and older-aged patients significantly improved the short-term clinical outcome and allowed patients to return to sporting activities similar to their preoperative levels.</p>
• 高純度硬化性ゲルを用いた低侵襲軟骨再生治療法の開発

  岩崎 倫政, 小野寺 智洋, 馬場 力哉 人工臓器 45 (1) 75 -77 2016年 [査読無し][通常論文]
  
• 人工膝関節再置換術後感染症治療経過中に薬剤性過敏症症候群を発症した1例

  高橋 要, 上徳 善太, 入江 徹, 笠原 靖彦, 小野寺 智洋, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 57 (1) 121 -122 2015年12月 [査読無し][通常論文]
  
• 特発性大腿骨頭壊死症患者のTHA術後満足度は可動域に影響される JHEQとJOA scoreを用いた検討

  入江 徹, 高橋 大介, 浅野 毅, 新井 隆太, 清藤 直樹, 中野 宏昭, 深谷 英昭, 小野寺 智洋, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 57 (1) 125 -126 2015年12月 [査読無し][通常論文]
  
• TKA術中のACL・半月板切除はkinematics patternに影響しない

  清藤 直樹, 小野寺 智洋, 笠原 靖彦, 西尾 悠介, 岩崎 倫政, 近藤 英司, 眞島 任史 北海道整形災害外科学会雑誌 57 (1) 131 -131 2015年12月 [査読無し][通常論文]
  
• Mobile bearing型TKAは術後non-medial pivot kinematics膝に対して膝蓋大腿関節圧を減少させる

  小野寺 智洋, 清藤 直樹, 西尾 悠介, 中野 宏昭, 深谷 英昭, 薮内 康史, 岩崎 倫政, 近藤 英司, 眞島 任史 北海道整形災害外科学会雑誌 57 (1) 131 -132 2015年12月 [査読無し][通常論文]
  
• 腫瘍性骨軟化症の3例

  山本 励志, 新井 隆太, 笠原 靖彦, 小野寺 智洋, 高橋 大介, 入江 徹, 浅野 毅, 清藤 直樹, 中野 宏昭, 深谷 英昭, 岩崎 倫政, 近藤 英司, 西尾 悠介, 安田 和則 北海道整形災害外科学会雑誌 57 (1) 176 -177 2015年12月 [査読無し][通常論文]
  
• 大腿骨転子間彎曲内反骨切り術における脚短縮理論モデルとその評価

  浅野 毅, 高橋 大介, 入江 徹, 新井 隆太, 清藤 直樹, 小野寺 智洋, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 57 (1) 180 -181 2015年12月 [査読無し][通常論文]
  
• 特発性大腿骨頭壊死症に併発した大腿骨頸部骨折に骨接合術で治療した2例

  深谷 英昭, 高橋 大介, 入江 徹, 浅野 毅, 新井 隆太, 清藤 直樹, 小野寺 智洋, 岩崎 倫政, 近藤 英司 北海道整形災害外科学会雑誌 57 (1) 183 -184 2015年12月 [査読無し][通常論文]
  
• 関節リウマチ患者の後足部変形に対する鏡視下足関節固定術の短期成績

  藤田 諒, 小野寺 智洋, 笠原 靖彦, 高橋 大介, 清藤 直樹, 浅野 毅, 新井 隆太, 近藤 英司, 岩崎 倫政 北海道整形災害外科学会雑誌 57 (1) 196 -197 2015年12月 [査読無し][通常論文]
  
• 恒久性膝蓋骨脱臼にGreen変法及びBlauth法合併手術を施行した1例

  濱崎 雅成, 小野寺 智洋, 清藤 直樹, 中野 宏明, 深谷 英明, 薮内 康史, 岩崎 倫政, 近藤 英司, 眞島 任史 北海道整形災害外科学会雑誌 57 (1) 201 -201 2015年12月 [査読無し][通常論文]
  
• リウマチ性前足部変形に対する切除関節形成術における術後再脱臼因子の検討

  中野 宏昭, 小野寺 智洋, 清藤 直樹, 深谷 英昭, 新井 隆太, 近藤 英司, 岩崎 倫政 北海道医学雑誌 90 (2) 144 -145 2015年11月 [査読無し][通常論文]
  
• 【整形外科領域における移植医療】 骨および軟骨に対する人工・生体材料、組織細胞移植の臨床成績 四肢外傷および傷病 大腿骨顆部特発性骨壊死に対する骨軟骨柱移植術の中・長期成績

  笠原 靖彦, 小野寺 智洋, 薮内 康史, 近藤 英司, 岩崎 倫政, 眞島 任史 別冊整形外科 (68) 130 -133 2015年10月 [査読無し][通常論文]
   

  大腿骨顆部特発性骨壊死に対して骨軟骨柱移植術を行った21例22膝の中・長期成績を検討した。対象は男6例6膝、女15例16膝で、手術時平均59歳、経過観察期間は平均10年であった。治療は骨軟骨柱移植術単独(M群)が16膝、高位脛骨骨切り術(HTO)+骨軟骨柱移植術併用(HM群)が6膝であった。全例で関節温存可能であった。手術年齢はM群で有意に高く、JOAスコアはHM群で有意に高く、術前後でROMに有意な変化はなかった。大腿脛骨角はM群で有意に内反変形が進行していた。MRI撮影しRoberts等の評価基準で行った軟骨評価では、両群間に有意差は認めなかった。
• 成績の評価に基づいたこれからの人工股関節置換術は? 当科における初回ハイブリッド人工股関節全置換術の長期成績

  入江 徹, 高橋 大介, 浅野 毅, 下段 俊, 亀田 敏明, 薮内 康史, 新井 隆太, 小野寺 智洋, 近藤 英司, 岩崎 倫政 日本関節病学会誌 34 (3) 332 -332 2015年10月 [査読無し][通常論文]
  
• 軟骨下脆弱性骨折の病態と治療 大腿骨頭軟骨下脆弱性骨折に対する免荷治療は効果があるのか 予後予測因子の検討

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• Lapidus変法において第1中足骨短縮量は母趾臨床成績を向上させる

  小野寺 智洋, 松岡 正剛, 山崎 修司, 近藤 英司, 岩崎 倫政 日本足の外科学会雑誌 36 (2) S260 -S260 2015年09月 [査読無し][通常論文]
  
• 内側楔状開大式高位脛骨骨切り術後のスポーツ復帰に関する検討

  薮内 康史, 近藤 英司, 小野寺 智洋, 亀田 敏明, 小野寺 純, 北村 信人, 安田 和則, 八木 知徳, 岩崎 倫政 日本整形外科スポーツ医学会雑誌 35 (4) 326 -326 2015年08月 [査読無し][通常論文]
  
• 前十字靱帯損傷および大腿骨外側顆骨軟骨損傷に対し解剖学的2束靱帯再建術・腸骨移植・自家培養軟骨移植を一期的に施行した1例

  清藤 直樹, 近藤 英司, 三上 将, 小野寺 智洋, 岩崎 倫政, 安田 和則 日本整形外科スポーツ医学会雑誌 35 (4) 351 -351 2015年08月 [査読無し][通常論文]
  
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• S-ROM THAにおけるZTT II cupライナーの長期成績

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• 関節リウマチによる高度外反変形膝と末期足関節症に対して一期的手術を施行した1例

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• 外傷性膝関節脱臼後に内反型OAが進行した1例

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• Medial Open-Wedge HTOにおいて用いる人工骨の臨床評価 ハイドロキシアパタイトvs β-リン酸三カルシウム

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• 膝窩動脈捕捉症候群に対し、観血的治療を行った1例

  藤田 諒, 笠原 靖彦, 入江 徹, 小野寺 智洋, 高橋 大介, 清藤 直樹, 浅野 毅, 新井 隆太, 近藤 英司, 岩崎 倫政 北海道整形災害外科学会雑誌 56 (2) 311 -311 2015年07月 [査読無し][通常論文]
  
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• 当科における単顆型人工膝関節置換術の短期成績 New Knee Society Scoreを用いて

  笠原 靖彦, 小野寺 智洋, 清藤 直樹, 高橋 大介, 浅野 毅, 新井 隆太, 岩崎 倫政, 西尾 悠介, 近藤 英司, 眞島 任史 北海道整形災害外科学会雑誌 56 (2) 315 -316 2015年07月 [査読無し][通常論文]
  
• 人工膝関節置換術において大腿骨顆部と脛骨後縁との相対的位置関係は屈曲拘縮の程度と相関する

  小野寺 智洋, 笠原 靖彦, 清藤 直樹, 高橋 大介, 浅野 毅, 新井 隆太, 岩崎 倫政, 西尾 悠介, 近藤 英司, 眞島 任史 北海道整形災害外科学会雑誌 56 (2) 319 -320 2015年07月 [査読無し][通常論文]
  
• TKA後の膝関節kinematics patternは術前のkinematics patternに依存する

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  膝離断性骨軟骨炎(OCD)の手術治療成績について報告した。治療を施行した膝OCD患者22例25膝28部位を対象とした。Tegner Activity scoreは平均6.3±1.4→6.1±1.4と活動性は維持された。Juvenile typeに限ると平均7.0±0.6→6.9±0.5と活動性は高いレベルで維持されていた。Adult typeに限ると平均5.5±1.6→5.2±1.5であった。Lysholm scoreは平均72.4±15.6→97.1±と9.1と有意に改善し、juvenile type、adult typeも術前に比べて術後は有意に改善した。
• 遺残組織を温存した解剖学的2束前十字靱帯再建術の臨床成績 従来の非温存再建術との比較

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• 人工膝関節置換術術中の前十字靱帯および内側半月板切除はkinematics patternに影響しない

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• 1C43 マイクロピペット吸引法を用いたスフィンゴ糖脂質欠損マウス膝軟骨細胞の力学特性評価(OS2-4:細胞・分子のバイオメカニクス(4))

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• 大腿骨顆部特発性骨壊死に対する骨軟骨柱移植術の治療成績

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• 高純度アルギン酸ゲルと多段階分化誘導法を併用したマウスiPS細胞軟骨分化誘導法の確立

  本谷和俊, 小野寺智洋, 寺島理代, 門間太輔, 松岡正剛, 馬場力哉, 上徳善太, 松原新史, 宝満健太郎, 岩崎倫政 日本整形外科学会雑誌 89 (8) S1730 -S1730 2015年 [査読無し][通常論文]
  
• ガングリオシド欠損は軟骨の肥大化を抑制することにより関節軟骨修復を促進する

  松岡正剛, 小野寺智洋, 笹沢史生, 門間太輔, 馬場力哉, 本谷和俊, 上徳善太, 松原新史, 宝満健太郎, 岩崎倫政 日本整形外科学会雑誌 89 (8) S1710 -S1710 2015年 [査読無し][通常論文]
  
• 軟骨細胞上の高マンノース型糖鎖構造の減少は軟骨変性を促進する

  宝満健太郎, 小野寺智洋, 門間太輔, 松岡正剛, 馬場力哉, 本谷和俊, 松原新史, 上徳善太, 岩崎倫政 日本整形外科学会雑誌 89 (8) S1533 -S1533 2015年 [査読無し][通常論文]
  
• 人工膝関節置換術後の大腿骨コンポーネント後顆の厚みは屈曲拘縮のリスクになりうる

  小野寺 智洋, 笠原 靖彦, 高橋 大介, 眞島 任史, 西池 修, 近藤 英司, 安田 和則 北海道整形災害外科学会雑誌 56 (1) 61 -62 2014年12月 [査読無し][通常論文]
  
• 当科における膝離断性骨軟骨炎の手術治療成績

  西尾 悠介, 笠原 靖彦, 小野寺 智洋, 高橋 大介, 浅野 毅, 新井 隆太, 清藤 直樹, 岩崎 倫政, 近藤 英司, 安田 和則, 眞島 任史 北海道整形災害外科学会雑誌 56 (1) 67 -67 2014年12月 [査読無し][通常論文]
  
• リウマチ性前足部変形の関節破壊評価に対する超音波検査の有用性

  小野寺 智洋, 綛村 俊之, 笠原 靖彦, 西尾 悠介, 高橋 大介, 岩崎 倫政, 眞島 任史, 近藤 英司, 安田 和則 北海道整形災害外科学会雑誌 56 (1) 78 -78 2014年12月 [査読無し][通常論文]
  
• リウマチ性前足部変形において外側趾の脱臼は高度関節破壊を示唆する

  鈴木 裕貴, 小野寺 智洋, 笠原 靖彦, 高橋 大介, 西尾 悠介, 清藤 直樹, 浅野 毅, 新井 隆太, 岩崎 倫政, 眞島 任史, 近藤 英司, 安田 和則 北海道整形災害外科学会雑誌 56 (1) 78 -79 2014年12月 [査読無し][通常論文]
  
• 特発性大腿骨頭壊死症におけるテリパラチドの骨頭圧潰進行抑制効果の検討

  新井 隆太, 高橋 大介, 入江 徹, 浅野 毅, 笠原 靖彦, 小野寺 智洋, 清藤 直樹, 岩崎 倫政, 井上 正弘, 紺野 拓也, 西尾 悠介, 近藤 英司, 安田 和則, 眞島 任史 北海道整形災害外科学会雑誌 56 (1) 121 -122 2014年12月 [査読無し][通常論文]
  
• 偏心性寛骨臼回転骨切り術による大腿骨頭の遠位化と内方化

  入江 徹, 高橋 大介, 浅野 毅, 新井 隆太, 笠原 靖彦, 小野寺 智洋, 清藤 直樹, 岩崎 倫政, 井上 正弘, 紺野 拓也, 西尾 悠介, 近藤 英司, 安田 和則, 眞島 任史 北海道整形災害外科学会雑誌 56 (1) 123 -123 2014年12月 [査読無し][通常論文]
  
• リウマチ性前足部変形に対する切除関節形成術:術後再脱臼関連因子の検討

  中野 宏昭, 小野寺 智洋, 清藤 直樹, 深谷 英昭, 新井 隆太, 近藤 英司, 岩崎 倫政 日本足の外科学会雑誌 35 (2) S258 -S258 2014年10月 [査読無し][通常論文]
  
• Ilizarov創外固定器を用いた足関節固定術の疾患別検討

  深谷 英昭, 小野寺 智洋, 清藤 直樹, 中野 宏昭, 新井 隆太, 高橋 大介, 近藤 英司, 岩崎 倫政 日本足の外科学会雑誌 35 (2) S280 -S280 2014年10月 [査読無し][通常論文]
  
• 変形性足関節症とリウマチ性足関節症における鏡視下足関節固定術の臨床成績の比較

  小野寺 智洋, 清藤 直樹, 深谷 英昭, 中野 宏昭, 新井 隆太, 藤田 諒, 近藤 英司, 岩崎 倫政 日本足の外科学会雑誌 35 (2) S280 -S280 2014年10月 [査読無し][通常論文]
  
• 足趾腫瘍による腫瘍性骨軟化症の1例

  新井 隆太, 小野寺 智洋, 高橋 大介, 入江 徹, 浅野 毅, 清藤 直樹, 中野 宏昭, 深谷 英昭, 近藤 英司, 岩崎 倫政 日本足の外科学会雑誌 35 (2) S299 -S299 2014年10月 [査読無し][通常論文]
  
• 偏心性寛骨臼回転骨切り術による大腿骨頭の遠位化と内方化

  入江 徹, 高橋 大介, 井上 正弘, 浅野 毅, 新井 隆太, 安藤 亮, 紺野 拓也, 小野寺 智洋, 近藤 英司, 岩崎 倫政 東日本整形災害外科学会雑誌 26 (3) 267 -267 2014年08月 [査読無し][通常論文]
  
• TKA術中のACL・半月板切除はkinematics patternに影響しない

  清藤 直樹, 小野寺 智洋, 笠原 靖彦, 西尾 悠介, 近藤 英司, 眞島 任史, 岩崎 倫政 日本整形外科学会雑誌 88 (8) S1499 -S1499 2014年08月 [査読無し][通常論文]
  
• 大腿骨転子間彎曲内反骨切り術における脚短縮理論モデルとその評価

  浅野 毅, 高橋 大介, 新井 隆太, 笠原 靖彦, 小野寺 智洋, 近藤 英司, 清藤 直樹, 岩崎 倫政 日本整形外科学会雑誌 88 (8) S1542 -S1542 2014年08月 [査読無し][通常論文]
  
• リウマチ性足関節症に対する鏡視下足関節固定術の治療成績

  小野寺 智洋, 藤田 諒, 笠原 靖彦, 清藤 直樹, 近藤 英司, 岩崎 倫政 JOSKAS 39 (4) 359 -359 2014年07月 [査読無し][通常論文]
  
• 膝関節複合靱帯損傷後内側型OAに対して脛骨骨切り術を施行した1例

  清藤 直樹, 小野寺 智洋, 笠原 靖彦, 近藤 英司, 岩崎 倫政, 眞島 任史 JOSKAS 39 (4) 425 -425 2014年07月 [査読無し][通常論文]
  
• 当科における膝離断性骨軟骨炎の手術治療成績

  西尾 悠介, 笠原 靖彦, 小野寺 智洋, 高橋 大介, 浅野 毅, 清藤 直樹, 新井 隆太, 近藤 英司, 岩崎 倫政, 眞島 任史 JOSKAS 39 (4) 496 -496 2014年07月 [査読無し][通常論文]
  
• 関節リウマチ患者の後足部変形に対する鏡視下足関節固定術の短期成績

  藤田 諒, 小野寺 智洋, 笠原 靖彦, 高橋 大介, 清藤 直樹, 浅野 毅, 新井 隆太, 近藤 英司, 岩崎 倫政 北海道医学雑誌 89 (1) 90 -90 2014年05月 [査読無し][通常論文]
  
• TKA後の膝関節kinematicsは術前のkinematicsに依存する

  清藤 直樹, 笠原 靖彦, 小野寺 智洋, 西尾 悠介, 高橋 大介, 浅野 毅, 新井 隆太, 近藤 英司, 眞島 任史, 岩崎 倫政 日本整形外科学会雑誌 88 (2) S60 -S60 2014年03月 [査読無し][通常論文]
  
• 人工膝関節置換術において大腿骨顆部と脛骨後縁との相対的位置関係は伸展可動域と相関する

  小野寺 智洋, 笠原 靖彦, 西尾 悠介, 清藤 直樹, 近藤 英司, 高橋 大介, 岩崎 倫政, 眞島 任史 日本整形外科学会雑誌 88 (2) S62 -S62 2014年03月 [査読無し][通常論文]
  
• 偏心性寛骨臼回転骨切り術による大腿骨頭の遠位化と内方化

  入江 徹, 高橋 大介, 井上 正弘, 紺野 拓也, 浅野 毅, 新井 隆太, 笠原 靖彦, 小野寺 智洋, 近藤 英司, 眞島 任史, 岩崎 倫政 日本整形外科学会雑誌 88 (2) S257 -S257 2014年03月 [査読無し][通常論文]
  
• 大腿骨顆部特発性骨壊死に対する骨軟骨柱移植術の中〜長期成績

  笠原 靖彦, 小野寺 智洋, 西尾 悠介, 清藤 直樹, 高橋 大介, 近藤 英司, 岩崎 倫政, 眞島 任史 日本整形外科学会雑誌 88 (2) S294 -S294 2014年03月 [査読無し][通常論文]
  
• 当科における膝離断性骨軟骨炎の手術治療成績

  西尾 悠介, 笠原 靖彦, 小野寺 智洋, 高橋 大介, 浅野 毅, 新井 隆太, 清藤 直樹, 近藤 英司, 安田 和則, 眞島 任史, 岩崎 倫政 日本整形外科学会雑誌 88 (2) S298 -S298 2014年03月 [査読無し][通常論文]
  
• テリパラチドは特発性大腿骨頭壊死症の骨頭圧潰進行を抑制する

  新井 隆太, 高橋 大介, 井上 正弘, 入江 徹, 紺野 拓也, 浅野 毅, 笠原 靖彦, 小野寺 智洋, 清藤 直樹, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 88 (3) S633 -S633 2014年03月 [査読無し][通常論文]
  
• 人工膝関節インプラントにおいてposterior condylar offsetと脛骨後縁との相対的位置関係は屈曲拘縮のリスクになりうる

  小野寺 智洋, 笠原 靖彦, 西池 修, 西尾 悠介, 清藤 直樹, 高橋 大介, 近藤 英司, 岩崎 倫政, 眞島 任史 日本整形外科学会雑誌 88 (3) S844 -S844 2014年03月 [査読無し][通常論文]
  
• 人工股関節置換術後のJHEQとJOA scoreの比較検討

  太田 昌博, 高橋 大介, 浅野 毅, 辻本 武尊, 小野寺 智洋, 笠原 靖彦, 近藤 英司, 岩崎 倫政 日本整形外科学会雑誌 88 (3) S963 -S963 2014年03月 [査読無し][通常論文]
  
• 遺残組織温存解剖学的2束前十字靱帯再建術の臨床成績 連続した161例による非温存再建との前向き比較研究

  小野寺 純, 近藤 英司, 北村 信人, 河口 泰之, 横田 正司, 西尾 悠介, 笠原 靖彦, 小野寺 智洋, 岩崎 倫政, 安田 和則 日本整形外科学会雑誌 88 (3) S1003 -S1003 2014年03月 [査読無し][通常論文]
  
• 外傷性膝関節脱臼後の内反膝の1例

  清藤 直樹, 笠原 靖彦, 小野寺 智洋, 高橋 大介, 浅野 毅, 新井 隆太, 西尾 悠介, 近藤 英司, 眞島 任史 北海道整形災害外科学会雑誌 55 (2) 372 -372 2014年03月 [査読無し][通常論文]
  
• スフィンゴ糖脂質の欠損は変形性関節症の進行を助長する

  清藤直樹, 山下匡, 佃幸憲, 松井雄一郎, 瓜田淳, 小野寺智洋, 藤谷直樹, 篠原康郎, 三浪明男, 岩崎倫政 日本軟骨代謝学会プログラム・抄録集 27th 78 2014年 [査読無し][通常論文]
  
• 側臥位OCM approachの安定したインプラント設置における術前ガイドワイヤー使用の有用性

  浅野 毅, 髙橋 大介, 入江 徹, 紺野 拓也, 新井 隆太, 近藤 英司, 笠原 靖彦, 小野寺 智洋, 西尾 悠介, 岩崎 倫政 日本人工関節学会プログラム・抄録集 44th 423 2014年 [査読無し][通常論文]
  
• 4ケ月健診はDDHスクリーニングに適切な時期なのか?

  髙橋 大介, 浅野 毅, 井上 正弘, 入江 徹, 紺野 拓也, 新井 隆太, 西尾 悠介, 小野寺 智洋, 笠原 靖彦, 近藤 英司, 岩崎 倫政 北海道整形災害外科学会 126th 60 2014年 [査読無し][通常論文]
  
• 当科における単顆型人工膝関節置換術の短期成績―New Knee Society Scoreを用いて―

  笠原 靖彦, 小野寺 智洋, 西尾 悠介, 清藤 直樹, 髙橋 大介, 浅野 毅, 新井 隆太, 近藤 英司, 岩崎 倫政, 眞島 任史 日本人工関節学会プログラム・抄録集 44th 523 2014年 [査読無し][通常論文]
  
• TKA後のkinematicsと術後の臨床成績の関係

  西尾 悠介, 笠原 靖彦, 小野寺 智洋, 髙橋 大介, 浅野 毅, 清藤 直樹, 新井 隆太, 近藤 英司, 岩崎 倫政, 眞島 任史 日本人工関節学会プログラム・抄録集 44th 383 2014年 [査読無し][通常論文]
  
• S​‐​R​O​M​ ​T​H​A​に​お​け​る​Z​T​T​ ​I​I​ ​c​u​p​ラ​イ​ナ​ー​の​長​期​成​績

  新井 隆太, 髙橋 大介, 井上 正弘, 入江 徹, 紺野 拓也, 浅野 毅, 笠原 靖彦, 小野寺 智洋, 清藤 直樹, 西尾 悠介, 近藤 英司, 安田 和則, 眞島 任史, 岩崎 倫政 北海道整形災害外科学会 126th 72 2014年 [査読無し][通常論文]
  
• 偽関節になった足舟状骨疲労骨折に対する治療経験 1例報告

  内田 淳, 安藤 亮, 松原 新史, 門司 順一, 小野寺 智洋, 大西 貴士 日本足の外科学会雑誌 34 (2) S344 -S344 2013年10月 [査読無し][通常論文]
  
• 当科における膝離断性骨軟骨炎の手術治療成績

  西尾 悠介, 笠原 靖彦, 小野寺 智洋, 高橋 大介, 浅野 毅, 新井 隆太, 清藤 直樹, 近藤 英司, 安田 和則, 岩崎 倫政, 眞島 任史 日本臨床スポーツ医学会誌 21 (4) S245 -S245 2013年10月 [査読無し][通常論文]
  
• 外反母趾における第1TMT関節外転不安定性の検討

  小野寺 智洋, 笠原 靖彦, 山崎 修司, 門司 順一, 岩崎 倫政, 近藤 英司, 安田 和則, 眞島 任史 日本足の外科学会雑誌 34 (2) S183 -S183 2013年10月 [査読無し][通常論文]
  
• リウマチ性前足部変形において外側趾の脱臼は高度関節破壊を示唆する

  鈴木 裕貴, 小野寺 智洋, 笠原 靖彦, 西尾 悠介, 清藤 直樹, 岩崎 倫政, 近藤 英司, 安田 和則, 眞島 任史 日本足の外科学会雑誌 34 (2) S207 -S207 2013年10月 [査読無し][通常論文]
  
• 膝屈筋腱を用いた解剖学的2束前十字靱帯再建術後の骨孔径変化 CTを用いた研究

  近藤 英司, 河口 泰之, 小野寺 純, 北村 信人, 小野寺 智洋, 笠原 靖彦, 高橋 大介, 岩崎 倫政, 安田 和則 東日本整形災害外科学会雑誌 25 (3) 360 -360 2013年08月 [査読無し][通常論文]
  
• 臨床 人工膝関節全置換術後感染における二期的再置換術の治療成績

  辻本 武尊, 笠原 靖彦, 小野寺 智洋 整形・災害外科 56 (9) 1185 -1191 2013年08月 [査読無し][通常論文]
  
• 最新原著レビュー 人工膝関節全置換術におけるトラネキサム酸を用いたドレーンクランプ法による深部静脈血栓症のリスク

  小野寺 智洋 整形外科 = Orthopedic surgery 64 (6) 589 -591 2013年06月 [査読無し][通常論文]
  
• 膝関節発生びまん型色素性絨毛結節性滑膜炎に対する滑膜全切除術の治療成績

  校條 祐輔, 笠原 靖彦, 小野寺 智洋, 高橋 大介, 綛村 俊之, 入江 徹, 眞島 任史 JOSKAS 38 (2) 318 -319 2013年04月25日
• 膝蓋腱を使用した前十字靭帯再々建術後2年で生じた膝蓋腱断裂の1例

  藤田 勝久, 笠原 靖彦, 入江 徹, 綛村 俊之, 高橋 大介, 小野寺 智洋, 眞島 任史 JOSKAS 38 (2) 314 -315 2013年04月25日
• 高度内反変形膝に対する全人工膝関節置換術の治療成績

  岩崎 浩司, 笠原 靖彦, 小野寺 智洋, 高橋 大介, 綛村 俊之, 入江 徹, 眞島 任史 JOSKAS 38 (2) 230 -231 2013年04月25日
• 距骨骨軟骨病変に対する骨軟骨柱移植術の治療成績

  小野寺 智洋, 眞島 任史, 笠原 靖彦, 高橋 大介, 入江 徹, 綛村 俊之, 須々田 幸一 JOSKAS 38 (1) 146 -147 2013年03月25日
• リウマチ性前足部変形に対する切除関節形成術 MP関節術後再脱臼関連因子の検討

  大西 貴士, 小野寺 智洋, 笠原 靖彦, 入江 徹, 綛村 俊之, 三浪 明男, 眞島 任史, 高橋 大介 北海道整形災害外科学会雑誌 54 (2) 330 -331 2013年03月 [査読無し][通常論文]
  
• 関節疾患に対する新規注射用治療製剤 (特集 次世代健康・医療・福祉の科学)

  小野寺 智洋, 岩崎 倫政, 五十嵐 達弥 化學工業 64 (1) 46 -50 2013年01月
• Btbd7/Cleftin regulates cleft formation and branching morphogenesis of epithelial cells

  Sakai Takayoshi, Onodera Tomohiro Journal of Oral Biosciences 55 (2) 73 -75 2013年 [査読無し][通常論文]
   

  During embryonic development, many internal organs are formed through repetitive branching morphogenesis of epithelial tissue. In this study, we determined that the fibronectin-induced gene BTB (POZ) domain containing 7 (Btbd7)/Cleftin plays an important role in this process, and we analyzed how this gene regulates cleft formation. We demonstrated that branching morphogenesis of epithelial tissue is controlled by Btbd7/Cleftin during salivary gland and lung formation. Btbd7/Cleftin, acting through other proteins such as E-cadherin and Snail2, regulates the branching morphogenesis of the epithelial cells that form a cleft structure. Furthermore, disruption of cell-cell adhesion by Btbd7/Cleftin induces epithelial cell migration, which is required for branching morphogenesis of the organ. © 2013 Japanese Association for Oral Biology.
• リウマチ性前足部変形に対する第1MTP関節固定術、第2-5MTP関節切除形成術における術後予後不良因子の検討

  小野寺 智洋, 田辺 明彦, 大西 貴士, 沢口 直弘, 渡部 琢哉, 笠原 靖彦, 高橋 大介, 眞島 任史 日本関節病学会誌 31 (3) 371 -371 2012年10月 [査読無し][通常論文]
  
• スフィンゴ糖脂質の欠損は変形性関節症の進行を助長する

  清藤直樹, 山下匡, 佃幸憲, 松井雄一郎, 瓜田淳, 小野寺智洋, 藤谷直樹, 篠原康郎, 三浪明男, 岩崎倫政 日本整形外科学会雑誌 86 (8) S1330 2012年08月25日 [査読無し][通常論文]
  
• リウマチ性前足部変形に対する第1MTP関節固定術、第2-5MTP関節切除形成術における術後予後不良因子の検討

  小野寺 智洋, 田辺 明彦, 大西 貴士, 沢口 直弘, 渡部 琢哉, 笠原 靖彦, 高橋 大介, 三浪 明男, 眞島 任史 北海道医学雑誌 87 (4-5) 215 -216 2012年08月 [査読無し][通常論文]
  
• ガングリオシドGM3は関節リウマチの発症,進行において重要な役割を担う

  佃幸憲, 岩崎倫政, 清藤直樹, 金山剛士, 藤谷直樹, 篠原康郎, 笠原靖彦, 小野寺智洋, 浅野毅, 三浪明男, 山下匡 日本整形外科学会雑誌 86 (8) 2012年
• 変形性関節症におけるスフィンゴ糖脂質の機能解析

  清藤直樹, 岩崎倫政, 佃幸憲, 松井雄一郎, 瓜田淳, 小野寺智洋, 三浪明男, 山下匡, 藤谷直樹, 篠原康郎 北海道整形災害外科学会 122nd 1 2012年 [査読無し][通常論文]
  
• マウスにおけるスフィンゴ糖脂質の欠損は変形性関節症の進行を助長する

  清藤直樹, 山下匡, 佃幸憲, 松井雄一郎, 瓜田淳, 小野寺智洋, 藤谷直樹, 篠原康郎, 三浪明男, 岩崎倫政 日本軟骨代謝学会プログラム・抄録集 25th 107 2012年 [査読無し][通常論文]
  
• ガングリオシドGM3は関節リウマチの発症,進行において重要な役割を担う。

  佃幸憲, 岩崎倫政, 清藤直樹, 笠原靖彦, 小野寺智洋, 浅野毅, 三浪明男, 金山剛士, 藤谷直樹, 篠原康郎, 鈴木孝治, 山下匡 北海道整形災害外科学会 122nd 1 2012年 [査読無し][通常論文]
  
• Xa阻害活性測定による合成Xa阻害剤モニタリングの試み

  小野寺 智洋, 眞島 任史, 笠原 靖彦, 松野 一彦, 遠見 真理, 三浪 明男 東日本整形災害外科学会雑誌 = Journal of the Eastern Japan Association of Orthopaedics and Traumatology 23 (2) 258 -262 2011年06月30日 [査読無し][通常論文]
  
• Cleftin: A novel fibronectin-induced gene that promotes branching morphogenesis

  Tomohiro Onodera, Takayoshi Sakai, Kenneth M. Yamada DEVELOPMENTAL BIOLOGY 319 (2) 505 -505 2008年07月 [査読無し][通常論文]
  

書籍等出版物

• 明日の足診療シリーズⅡ 足の腫瘍性病変・小児疾患の診かた

  小野寺智洋 (担当:分担執筆範囲:巻き趾、第Ⅱ章 第10節, 301-304)
  2021年
• 整形外科で起こるトラブルと求められる足場材料

  小野寺智洋 (担当:分担執筆範囲:第2章 第10 節, 生体吸収性材料の開発と安全性評価, 134-137)
  技術情報協会 2017年
• 関節リウマチの画像診断

  小野寺智洋 (担当:分担執筆範囲:進行期関節リウマチ 下肢, 136-158)
  メディカル・サイエンス・インターナショナル 2017年
• Processing and applications

  Manabu Ito, Tomohiro Onodera, Tadanao Funakoshi (担当:分担執筆)
  Springer 2015年 (ISBN: 9783662468418) x, 281 p.
• 整形外科で起こるトラブルと求められる足場材料

  小野寺智洋 (担当:分担執筆範囲:第1章 第5節, 体内埋め込み医療材料の開発とその理想的な性能・デザインの要件, 99-101)
  技術情報協会 2013年
• Current protocols in cell biology

  Tomohiro Onodera (担当:分担執筆範囲:Embryonic organ culture,)
  John Wiley 1998年 (ISBN: 0471241083) v. (looseleaf)

所属学協会

• 日本小児整形外科学会   日本足の外科学会   日本バイオマテリアル学会   東日本整形災害外科学会   日本関節病学会   日本軟骨代謝学会   日本リウマチ学会   米国整形外科基礎学会   International Cartilage Regeneration & Joint Preservation Society   日本人工関節学会   整形外科バイオマテリアル研究会   日本整形外科学会   北海道整形災害外科学会   

共同研究・競争的資金等の研究課題

• O-17標識水を用いたMRIによる関節軟骨病変の早期診断を可能とする革新的評価法の安全性および性能を検討する医師主導治験

  日本医療研究開発機構 令和6年度 「橋渡し研究プログラム」シーズC(a)

  研究期間 : 2024年04月 -2028年03月
• 機能性糖脂質分子を導入した新規軟骨再生材料の開発

  日本学術振興会：科学研究費助成事業

  研究期間 : 2024年02月 -2025年03月 

  代表者 : 小野寺 智洋
• 機能性糖脂質分子を導入した新規軟骨再生材料の開発

  日本学術振興会：科学研究費助成事業 基盤研究(B)

  研究期間 : 2022年04月 -2025年03月 

  代表者 : 小野寺 智洋, 長濱 宏治, 照川 アラー, 花松 久寿, 松岡 正剛
• 細胞架橋ゲルを基盤技術として用いた力学操作によるin vivo組織工学技術の確立

  日本学術振興会：科学研究費助成事業 基盤研究(B)

  研究期間 : 2022年04月 -2025年03月 

  代表者 : 長濱 宏治, 小野寺 智洋

   

  ヒト筋衛星細胞をアジド化マンノサミン存在下で2日間培養してアジド化筋衛星細胞を作製した後、複数のジベンゾシクロオクチン基 (DBCO) を導入した直鎖型アルギン酸とクリック架橋反応させることで筋衛星細胞ゲルを作製した。各種筋衛星細胞架橋ゲルおよびコントロールの筋衛星細胞内包ゲル（筋衛星細胞がアルギン酸が連結していない）に対して、10％のひずみが生じるように10分間だけ圧縮刺激を与えて核の形態変化を評価した結果、細胞架橋ゲル内の細胞核は大きく歪んで扁平化したが、細胞内包ゲルでは核扁平化は起こらなかったことより、細胞架橋ゲルでは与えたメカノシグナルが力学伝導体であるアルギン酸を介してゲル内の細胞に伝わり、細胞が受けたメカノシグナルがそのまま核に伝わり核が扁平化したと考えられる。骨格筋組織で発生する伸展刺激をin vitroで再現する伸展培養装置を用いて、筋衛星細胞架橋ゲルおよび内包ゲルを培養し、分化にかかる転写共役因子YAPの核内移行および筋分化について評価した。その結果、細胞架橋ゲルではYAPの核内移行が短時間で促進されたが、細胞内包ゲルではYAPの核内移行は見られなかった。以上の結果より、筋衛星細胞架橋ゲルでは、筋衛星細胞がメカノシグナルを生化学シグナルにトランスデュースするのではなく、メカノシグナルのままダイレクトに且つ素早く核に伝達することができることが分かった。大腿筋損傷マウスに筋衛星細胞架橋ゲルおよび細胞内包ゲルを投与し、筋力の変化および再建部位の組織学的評価を行った結果、細胞架橋ゲルでは元の筋力レベルまで回復が見られ、再建組織の大部分は筋線維であったが、細胞内包ゲルではほとんど筋力は回復せず、また線維化した組織が再建された。以上の動物実験はin vitro実験で得た知見と一致しており、力学刺激を活用することの意義を実証できた。
• O-17標識水を水トレーサーとして用いたMRIによる関節軟骨病変の早期診断を可能とする革新的評価法の確立と治験に向けた実用化研究

  日本医療研究開発機構(AMED) 革新的医療技術創出拠点プロジェクト橋渡し研究プログラムpreF

  研究期間 : 2022年04月 -2024年03月
• 幹細胞による安全な軟骨再生医療のための脂質分化マーカーの探索とその機能の解明

  日本学術振興会：科学研究費助成事業 基盤研究(C)

  研究期間 : 2018年04月 -2022年03月 

  代表者 : 馬場 力哉, 岩崎 倫政, 小野寺 智洋

   

  本研究はより安全で効率的なiPS細胞を用いた軟骨再生医療を実現することを目指した。①ヒトiPS細胞から軟骨細胞への分化過程で特徴的な構造変化を示す糖鎖が存在する。その糖鎖を制御することで、効率的な分化誘導法が確立できる。②細胞特異的糖鎖解析の手法を用いて、未分化iPS細胞特異的に細胞傷害性を持つ抗体R-17Fの至適使用条件を決定することができる、という2つの仮説を立てた。仮説①に対し、ヒトiPS細胞から軟骨細胞への分化過程でも、特徴的な構造変化を示す糖鎖が存在するかを検証した。その結果、マウスiPS細胞でみられたFucosyl GM1糖鎖のヒトでの発現はみられず、GM3の発現量は著しく減少し、一方GM1、GD3、GD1の発現は増加した。次に仮説②に対し、我々はAminolysis-SALSA法を応用することで、細胞特異的糖鎖解析を用いた軟骨細胞中に混入する未分化iPS細胞の検出、定量化法を確立し、またLNFP1糖鎖を認識し、細胞傷害性を持つ抗体R-17FのiPS細胞除去効果を糖鎖解析の手法で推察できることを明らかにした。また、iPS細胞と軟骨細胞の共培養にR-17F、二次抗体を添加することで、未分化iPS細胞を検出感度以下まで除去可能であることを明らかにし、さらに抗体処理した共培養細胞をSCID miceに移植し、teratomaが形成されないことを示した。仮説①に対しては、その成果を報告した(Liang Xu et al., Biomolecules,2020)。仮説②に対しては、細胞特異的糖鎖解析による未分化iPS細胞の検出、定量化法を確立し(Miyazaki et al., IJMS,2019)、また前述の方法にて、未分化iPS細胞除去の安全性も確認した。このiPS細胞の新たな検出法を用いた未分化iPS細胞の除去法の報告をもって、本研究の最終的な到達点と考えている。
• 外傷性膝関節軟骨損傷に対するO 17 標識水を用いた新たな質的評価法の確立

  JA 共済交通事故医療研究助成

  研究期間 : 2020年04月 -2022年03月
• 変形性関節症制圧を目指した新規免疫ネットワーク解析手法を用いた治療薬の開発

  文部科学省：科学研究費補助金(挑戦的研究(萌芽))

  研究期間 : 2017年06月 -2020年03月 

  代表者 : 小野寺智洋

   

  軟骨破片、マクロファージ、軟骨細胞・滑膜細胞を用いて、変形性関節症の関節内を模擬した共培養モデルを作成した。軟骨破砕片に関しては、組織破砕機を用いて実際の変形性関節症で見られる軟骨片と類似したサイズのものを作成することに成功した。本モデルを用いて、軟骨細胞・滑膜細胞の動態を観察したところ、153種類の遺伝子の統計学的有意な発現変化が見られた。現在はこれらの候補遺伝子のうち、特に関節炎と関係が深いと思われる最終候補遺伝子に対する機能解析を行っている。
• スフィンゴ糖脂質機能解析を基盤とした関節疾患の病態解明と新規治療法の開発

  日本学術振興会：科学研究費助成事業 基盤研究(B)

  研究期間 : 2016年04月 -2019年03月 

  代表者 : 岩崎 倫政, 高畑 雅彦, 小野寺 智洋, 前田 英次郎

   

  変形性関節症（OA）発症に関わる軟骨細胞上スフィンゴ糖脂質（GSLs)の分子基盤の解明と治療への応用を目指した。本プロジェクトでは、各種のGSLs関連合成酵素遺伝子ノックアウトマウス(KOマウス)を用いてIL-1刺激に対する各GSLｓ分子のシグナル制御機能を解析し、GSLs生合成経路の上流から下流に存在する主要な各GSLs分子が、OA発症に関わることを明らかにした。また、上流に存在するGSLs分子をKOすると重度のOAが発症することが明らかとなった。更に、軟骨細胞に対するメカニカル刺激に対し、GSLs分子はCaチャンネルを介して細胞内への刺激伝達を制御する機能を有することを証明した。
• 変形性関節症発症機序の解明に向けた軟骨変性制御機構における糖鎖機能解析

  日本学術振興会：科学研究費助成事業 挑戦的萌芽研究

  研究期間 : 2016年04月 -2018年03月 

  代表者 : 岩崎 倫政, 小野寺 智洋, 宝満 健太郎

   

  申請者は、これまでの研究成果より軟骨変性早期の糖鎖変化は可逆的変化であるという仮説を立てた。本研究では、in vivoおよびin vitro軟骨変性モデルを用いてこの仮説を証明し、変形性関節症（OA）早期の病態解明と新規治療法開発のための分子基盤の構築を目指した。 ウサギ膝関節内および軟骨器官培養系への高マンノース型糖鎖分解酵素の投与により早期OA変化を導くという結果を得た。さらに、酵素の投与中止により組織変性は回復を認めた。今後、得られた可逆的OA修復メカニズムを解明するための研究を推進していく方針である。
• 人工膝・股関節置換術適用患者の荷重免荷型トレッドミルにおける三次元歩行計測

  日本学術振興会：科学研究費助成事業 基盤研究(C)

  研究期間 : 2015年04月 -2018年03月 

  代表者 : 入江 徹, 小野寺 智洋, 遠山 晴一

   

  変形性股関節症に対し人工関節置換術を施行された患者の三次元歩行計測を，荷重免荷型トレッドミルを用いて50%荷重，75%荷重，100%荷重条件下で，術前，術後3週，術後6週に行った． 術後3週時，膝関節最大伸展角が50%荷重では100%荷重と比較し有意に増加し，術後6週時は50%荷重では100%荷重と比較し，股関節最大屈曲角が有意に減少し，膝関節最大伸展角が有意に増加した．荷重免荷型トレッドミルを用いてのリハビリテーションは，術前から術後3週で有効性が高く，蹴り出し力低下等の影響が出ないように実施するためには，75%程度までの荷重量を維持して実施した方が有効性が高い可能性があることが示された．
• 超高分子量ヒアルロン酸の関節軟骨マトリクスに与える影響の機序の解明と制御

  日本学術振興会：科学研究費助成事業 基盤研究(C)

  研究期間 : 2014年04月 -2017年03月 

  代表者 : 船越 忠直, 東藤 正浩, 岩崎 倫政, 小野寺 智洋

   

  変形性関節症に対する保存加療において用いられるヒアルロン酸製剤の分子量に依存する効果の違いについては不明な点が多い。ウサギ膝変形性関節症モデルを用いて異なる分子量（低分子量ヒアルロン酸(0.8 x 106 Da)および超高分子量ヒアルロン酸(6 x 106 Da）のヒアルロン酸がもつ軟骨保護作用について肉眼的、組織学的、生体力学的に検討した。超高分子量ヒアルロン酸は、優れた軟骨保護作用と低摩擦係数を示した。本研究結果により超高分子量ヒアルロン酸投与は軟骨表面の保護作用を有し、関節症性変化の進行抑制する可能性が示唆された。
• マウスiPS細胞から軟骨細胞への分化制御機能を有する糖鎖マーカーの探索

  日本学術振興会：科学研究費助成事業 挑戦的萌芽研究

  研究期間 : 2014年04月 -2016年03月 

  代表者 : 岩崎 倫政, 小野寺 智洋

   

  iPS細胞から軟骨細胞への分化に伴う細胞上の糖鎖構造解析を行った。分化に伴い複数の糖鎖分子において構造変化を認めた。これらの糖鎖分子の分化における機能的重要性が示唆された。 糖鎖は生物学的多様性を規定していると考えられており、糖鎖生物学的アプローチに基づく解析によりiPS細胞をはじめとする幹細胞の癌化や脱分化といった現象の解明が期待される。本研究成果を基盤として、今後は構造や発現変化を認めた糖鎖分子の分化および軟骨細胞の恒常性維持における機能を解析していく。
• 骨軟骨修復過程におけるスフィンゴ糖脂質の機能解析

  金原一郎記念医学医療振興財団研究助成

  研究期間 : 2015年04月 -2016年03月
• 損傷軟骨治癒過程におけるシンデカン-4の役割の解析

  文部科学省：科学研究費補助金(基盤研究(C))

  研究期間 : 2014年 -2016年 

  代表者 : 小野寺 智洋

   

  我々は幼若個体を用いることで、C57Bl/6マウスにおける軟骨全層修復モデルの確立に成功した（Matsuoka M, Onodera T et al., Tissue Eng. 2015）。本モデルは我々が独自に開発したモデルであり、本申請から発展した骨軟骨修復過程の病態解析として、糖脂質欠損マウスを用いた解析を行った（Matsuoka M, Onodera T et al., Sci Rep. 2017）。また、我々が本モデルを用いてシンデカン４欠損マウスを用いて検討を行ったところ、予想に反して野生型と比べシンデカン４欠損マウスの軟骨修復は促進される傾向を示した。
• 関節軟骨再生を目指した骨軟骨修復機構の解明

  整形災害外科学研究助成財団研究助成

  研究期間 : 2013年04月 -2014年03月
• 人工膝関節置換術における関節可動域を改善可能な可変型インサートの開発

  ノーステック財団 若手研究人材・ネットワーク育成補助金

  研究期間 : 2012年04月 -2013年03月
• ノックアウトマウスを用いたスフィンゴ糖脂質による骨・軟骨代謝調節機構の解明

  文部科学省：科学研究費補助金(挑戦的萌芽研究)

  研究期間 : 2012年 -2013年 

  代表者 : 岩崎 倫政, 高畑 雅彦, 小野寺 智洋

   

  細胞膜に存在するスフィンゴ糖脂質(glycosphingolipids、以下GSLs)は、効率的にシグナル伝達を行うためのラフト（rafts）と呼ばれる微小領域を細胞膜上に形成することで増殖、分化、活性化等の細胞機能を調節している。しかし、運動器細胞におけるGSLsの機能的役割は解明されていない。本研究では5系統の異なるGSLs関連合成酵素遺伝子ノックアウト(KO)マウスを用いてGSLsの骨および軟骨組織における機能の解明を目指す。本研究の目的は、①骨、軟骨の形成や機能・形態維持におけるGSLsの機能解析、②マウス骨、軟骨病態モデルにおけるGSLsの機能的役割の解明、の２項目である。GSLs関連合成酵素KOマウスでは明らかな発育・成長障害を認めなかったが、加齢に伴い有意な軟骨変性の進行が認められた。さらに、軟骨細胞は炎症性サイトカインであるIL-1に対する反応性が上昇しており、これに伴いMMP-13の発現やアポトーシスが亢進した。長管骨骨折モデルにおいては、GSLs関連合成酵素KOマウスにおいて内軟骨性骨化の遷延化が認められた。これらの結果より、GSLsは骨、軟骨組織の形成や発育には重要な機能を有していないことが明らかとなった。しかし、加齢や疾患の原因となる外部からの刺激に対しては、細胞に対する保護的機能を有することが示唆された。これらの成果は国内外での学会および海外ジャーナルにて発表済みである。
• 四肢形成、軟骨形成におけるBtbd7の機能解析

  文部科学省：科学研究費補助金(若手研究(B))

  研究期間 : 2011年 -2011年 

  代表者 : 小野寺 智洋

   

  Btbd7を中心とする遺伝子が軟骨形成において重要な役割を持つと仮定し、研究を進めた。マウス軟骨前駆細胞の軟骨分化誘導モデルにおけるBtbd7・Snail2・ケモカインSDF-1の発現レベルを比較したところ、一過性に上昇したのちに下降するという同様の発現パターンを示した。 そこでSDF-1に着目し、研究を推進した。SDF-1は軟骨修復過程において一過性に上昇し、SDF-1を含有したゲルを使用することによって、軟骨再生が誘導されることが明らかとなった。

産業財産権

• 特許第7191007号:線維軟骨組織損傷治療用組成物  

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• 特許第7191007号:線維軟骨組織損傷治療用組成物  

  近藤 英司, 岩崎 倫政, 小野寺 智洋, 金 佑泳, 河口 泰之  持田製薬株式会社, 国立大学法人北海道大学  202203013995074314
• 特開2022-071772:軟骨の損傷を検出するための造影剤、並びに当該造影剤を利用した軟骨の損傷を検査する方法及びプログラム  2022年05月16日

  小野寺 智洋, 工藤 與亮, 細川 吉暁, 岩崎 倫政, 亀田 浩之  国立大学法人北海道大学, 大陽日酸株式会社  202203014646185580
• 特開2021-109008:空間形成装置  2021年08月02日

  片山 政彦, 遠藤 俊弥, 小野寺 智洋, 岩崎 浩司, 岩崎 倫政  持田製薬株式会社, 国立大学法人北海道大学  202103003243779448
• 特開2021-109007:ニードル  2021年08月02日

  渡邊 望, 飯沼 和人, 田中 大地, 小野寺 智洋, 岩崎 浩司, 岩崎 倫政  持田製薬株式会社, 国立大学法人北海道大学  202103004674462472
• 特開2021-109006:注入装置  2021年08月02日

  渡邊 望, 水野 均, 日向野 理樹, 小野寺 智洋, 岩崎 浩司, 岩崎 倫政  持田製薬株式会社, 国立大学法人北海道大学  202103008424632866
• WO2018-159768:線維軟骨組織損傷治療用組成物  2018年09月07日

  近藤 英司, 岩崎 倫政, 小野寺 智洋, 金 佑泳, 河口 泰之  持田製薬株式会社, 国立大学法人北海道大学  202003009896045793
• 特開2018-023297:iPS細胞から軟骨細胞への分化判定方法及びiPS細胞から軟骨細胞への分化誘導を判定するための分化マーカー  2018年02月15日

  岩崎 倫政, 小野寺 智洋, 古川 潤一, 本谷 和俊  国立大学法人北海道大学  201803013919120584