Researcher basic information

• PhD, Purdue University, West Lafayette, 2009

• https://researchmap.jp/takasuka_te?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201401014411760951
• https://orcid.org/0000-0002-4413-2924
• http://www.agr.hokudai.ac.jp/takasuka/

• https://orcid.org/0000-0002-4413-2924

• 201401014411760951

• Life Science, Genome biology

• Bachelor's degree program, School of Agriculture
• Master's degree program, Graduate School of Global Food Resources
• Doctoral (PhD) degree program, Graduate School of Global Food Resources

Research activity information

• Endothelial cell-specific molecule 1 induced by interferon tau promotes angiogenesis in bovine endothelial cells.
  Hayato Naito; Kinari Kurata; Yasuhiro Yamazaki; Haruka Ukita; Hanako Bai; Manabu Kawahara; Taichi E Takasuka; Masashi Takahashi
  Theriogenology, 256, 117855, 117855, 05 Feb. 2026, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Interferon tau (IFNT), a pregnancy recognition factor in ruminants, maintains the corpus luteum and induces the expression of many genes in the uterus; however, the functions of these genes remain unclear. In this study, we investigated the effect of IFNT on endothelial cell-specific molecule 1 (ESM1) expression, along with the subsequent roles of ESM1 in bovine vascular endothelial cells in an in vitro cell culture environment. Quantitative polymerase chain reaction (qPCR) analysis showed that IFNT treatment significantly increased the expression levels of ESM1 in endometrial epithelial cells compared with those in non-treated control cells. qPCR and Western blot analyses showed an increasing trend in vascular endothelial growth factor (VEGF) expression following ESM1 treatment in bovine vascular endothelial cells. In addition, ESM1 treatment tended to increase cell proliferation and significantly increased the formation of tube-like structures of endothelial cells compared to those in non-treated control cells. These results suggest that IFNT induces ESM1 expression in endometrial epithelial cells. Moreover, ESM1 may contribute to angiogenesis via the expression of angiogenic factors, such as VEGF, in bovine endothelial cells.
• Multi-proteomics reveals integrated metabolic and regulatory networks for xylan catabolism in Streptomyces sp. SirexAA-E.
  Tatsuya Nagano; Keisuke Ohashi; Petra Banko; Vijay Kumar; Chiaki Hori; Brian G Fox; Taichi E Takasuka
  Microbiology spectrum, e0225125, 20 Nov. 2025, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, An insect-associated bacterium, Streptomyces sp. SirexAA-E (SirexAA-E) secretes plant biomass-degrading enzymes depending on the available cell wall materials. SirexAA-E readily utilizes xylan, one of the major hemicelluloses. However, the enzyme composition and pathways supporting xylan metabolism in SirexAA-E and other Streptomycetes have not been reported. We aimed to understand changes in both extracellular and intracellular protein productions by cultivating SirexAA-E in defined media containing either xylose, xylobiose, or xylan. Proteomics showed each carbon source gave specific extracellular protein composition when compared to glucose. Furthermore, intracellular proteomics using a pair-wise Tandem Mass Tag (TMT)-labeling LC-MS/MS identified 1,037 proteins with changes in the xylose-, xylobiose-, or xylan-dependent proteome relative to the glucose-derived proteome. We found that numerous proteins related to oxidative phosphorylation were enriched in the pentose-grown proteomes relative to the glucose proteome. This observation is consistent with a high demand for ATP to support protein secretion and intake of xylose and xylooligosaccharides. Additionally, several transporters for carbon uptake were identified in the genome of SirexAA-E by protein homology comparison with Streptomyces coelicolor, and several key transcriptional regulators used by SirexAA-E for catabolizing xylan were found by pull-down proteomics. The current study provides new insights into the extensive extracellular and intracellular responses of a cellulolytic Streptomyces to the major plant hemicellulose.IMPORTANCEStreptomyces sp. SirexAA-E can efficiently degrade cellulose, xylan, and mannan, the major polysaccharide components of woody biomass. Our previous work showed the relative simplicity of the secreted proteome used to degrade cellulose. In this study, we report on the extracellular and intracellular proteomic responses of SirexAA-E during growth on xylan. The substrate-specific proteomic profiles have given a new understanding of the regulation of xylanolytic enzymes and additional metabolic pathways supporting growth on a pentose sugar. These groupings of regulatory and structural proteins provide a blueprint for construction of more robust strains for biomass valorization., 51331192;31001902
• Mapping multi-substrate specificity of Arabidopsis aminotransferases.
  Kaan Koper; Marcos V V de Oliveira; Sebastian Huß; Shogo Hataya; Fayaz Soleymani; Charles Hawkins; Seung Y Rhee; Taichi E Takasuka; Zoran Nikoloski; Hiroshi A Maeda
  Nature plants, 11, 9, 1863, 1876, Sep. 2025, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal, Nitrogen is an essential element in all organisms, and its availability and use efficiency directly impact organismal growth and performance, especially in plants. Aminotransferases are core enzymes of the nitrogen metabolic network for synthesizing various organonitrogen compounds. Although each aminotransferase can potentially catalyse hundreds of transamination reactions with different combinations of amino and keto acid substrates, the full functionality of many aminotransferases remains elusive. Here we employed high-throughput gene synthesis and enzyme assay platforms to determine the substrate specificities of 38 aminotransferases of Arabidopsis thaliana and unveiled many previously unrecognized activities among a total of 4,104 reactions tested. The integration of these biochemical data in an enzyme-constrained metabolic model of Arabidopsis and in silico simulation further revealed that the promiscuity of aminotransferases may alter nitrogen distribution profiles and contribute to the robustness of the nitrogen metabolic network. This study provides foundational knowledge for deciphering the plant nitrogen metabolic network and improving nitrogen use efficiency in crops.
• Genome integration and expression of β-glucosidase in Priestia megaterium enhanced poly(3-hydroxybutyrate) production from cellobiose and cellulose.
  Vijay Kumar; Tatsuya Nagano; Taichi E Takasuka
  Bioresource technology, 132681, 132681, 14 May 2025, [Peer-reviewed], [Last author, Corresponding author], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal, Polyhydroxyalkanoates (PHAs) production using cellulosic biomass is a promising way for sustainable manufacturing of bioplastics. Priestia megaterium is an ideal choice as it can use glucose and xylose for PHA production. To further improve the strain for PHA production from cellobiose, we integrate exogenous β-glucosidase (Bgl) from Bacillus sp. GL1 (BsBgl) in the PHA depolymerase (phaZ1) deletion background (ΔZ1) using CRISPR-Cas. The deletion of phaZ1 in P. megaterium showed a significant improvement in the PHA accumulation whereas BsBgl expression resulted in robust activity and improved growth using cellobiose as a sole carbon source compared to other Bgl targets. To further improve the strain, four native promoters were examined for intracellular BsBgl expression, and the PHA promoter (PphaR) and citrate synthase promoter (Pcitz) showed 2.0- and 4.5-fold higher activities of BsBgl, compared to the xylose promoter (Pxyl). The rate of cellobiose utilization in engineered strains P2 (PphaRBsbgl_ΔZ1) and P4 (PcitzBsbgl_ΔZ1) was improved to 1.6-fold and 2.6-fold, whereas poly(3-hydroxybutyrate) (P3HB) yield for the respective strains was around 3-fold to the wild-type. The strain P2 turned out to be better for cellobiose to PHA production. Further, the strain P2 in a co-culture with cellulolytic Streptomyces sp. SirexAA-E in a consolidated bioprocessing yielded 76 mg of P3HB/ g of carboxymethylcellulose, which is 4.3-times higher than the co-culture with the wild-type. Thus, the present work improved the cellobiose utilization and P3HB accumulation of P. megaterium. The current study paves the way for designing efficient cell factories for cellulosic biomass into bioplastic in the future.
• Chondroprotective functions of neutrophil-derived extracellular vesicles by promoting the production of secreted frizzled-related protein 5 in cartilage.
  Keita Kitahara; Taku Ebata; Chen Liyile; Yoshio Nishida; Yuki Ogawa; Taiki Tokuhiro; Junki Shiota; Tatsuya Nagano; Taichi E Takasuka; Tsutomu Endo; Tomohiro Shimizu; Hend Alhasan; Tsuyoshi Asano; Daisuke Takahashi; Kentaro Homan; Tomohiro Onodera; Ken Kadoya; M Alaa Terkawi; Norimasa Iwasaki
  Cell communication and signaling : CCS, 22, 1, 569, 569, 27 Nov. 2024, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: Osteoarthritis (OA) is the most common degenerative joint disease characterized by cartilage degradation and various degrees of inflammation in the synovium. Growing evidence highlights that neutrophil extracellular vesicles (EVs) play a protective role in arthritic joints by promoting the resolution of inflammation and the synthesis of proteoglycans in cartilage. However, this homeostatic function is dependent on the activation state of neutrophils and the surrounding environment/tissues. Hence, we explored the chondroprotective functions of neutrophil-derived EVs under different stimulation conditions and the underlying molecular mechanism. METHODS: Human blood-derived neutrophils, murine bone marrow-derived neutrophils, C-28I2 cells and primary chondrocytes were used. Neutrophils were stimulated with different cytokines, and their EVs were isolated for chondrocyte stimulation and further subjected to RNA-sequencing analysis. Two experimental murine OA models were used, and the treatment was performed by intraarticular injections. RESULTS: Conditioned medium from neutrophils stimulated with TGF-β (N-β) had the greatest inhibitory effect on the expression of catabolic factors in stimulated chondrocytes. These protective effects were not impaired when conditioned medium of N-β from AnxA1-deficient mice was used. Consistent with these results, EVs isolated from N-β significantly reduced the expression of catabolic factors in stimulated chondrocytes. Bulk RNA-seq analysis revealed that secreted frizzled-related protein 5 (SFRP5) is upregulated in N-β-EV-stimulated chondrocytes. Furthermore, recombinant SFRP5 treatment significantly reduced the expression of catabolic factors in vitro and catabolic process in experimental murine OA models. CONCLUSIONS: The current study emphasizes the potential therapeutic application of neutrophils in OA and provides new knowledge on the molecular mechanisms underlying their function.
• Identification and characterization of methoxy- and dimethoxyhydroquinone 1,2-dioxygenase from Phanerochaete chrysosporium.
  Hiroyuki Kato; Yasushi Takahashi; Hiromitsu Suzuki; Keisuke Ohashi; Ryunosuke Kawashima; Koki Nakamura; Kiyota Sakai; Chiaki Hori; Taichi E Takasuka; Masashi Kato; Motoyuki Shimizu
  Applied and environmental microbiology, e0175323, 23 Jan. 2024, [Peer-reviewed], [International Magazine]
  English, Scientific journal, White-rot fungi, such as Phanerochaete chrysosporium, are the most efficient degraders of lignin, a major component of plant biomass. Enzymes produced by these fungi, such as lignin peroxidases and manganese peroxidases, break down lignin polymers into various aromatic compounds based on guaiacyl, syringyl, and hydroxyphenyl units. These intermediates are further degraded, and the aromatic ring is cleaved by 1,2,4-trihydroxybenzene dioxygenases. This study aimed to characterize homogentisate dioxygenase (HGD)-like proteins from P. chrysosporium that are strongly induced by the G-unit fragment of vanillin. We overexpressed two homologous recombinant HGDs, PcHGD1 and PcHGD2, in Escherichia coli. Both PcHGD1 and PcHGD2 catalyzed the ring cleavage in methoxyhydroquinone (MHQ) and dimethoxyhydroquinone (DMHQ). The two enzymes had the highest catalytic efficiency (kcat/Km) for MHQ, and therefore, we named PcHGD1 and PcHGD2 as MHQ dioxygenases 1 and 2 (PcMHQD1 and PcMHQD2), respectively, from P. chrysosporium. This is the first study to identify and characterize MHQ and DMHQ dioxygenase activities in members of the HGD superfamily. These findings highlight the unique and broad substrate spectra of PcHGDs, rendering them attractive candidates for biotechnological applications.IMPORTANCEThis study aimed to elucidate the properties of enzymes responsible for degrading lignin, a dominant natural polymer in terrestrial lignocellulosic biomass. We focused on two homogentisate dioxygenase (HGD) homologs from the white-rot fungus, P. chrysosporium, and investigated their roles in the degradation of lignin-derived aromatic compounds. In the P. chrysosporium genome database, PcMHQD1 and PcMHQD2 were annotated as HGDs that could cleave the aromatic rings of methoxyhydroquinone (MHQ) and dimethoxyhydroquinone (DMHQ) with a preference for MHQ. These findings suggest that MHQD1 and/or MHQD2 play important roles in the degradation of lignin-derived aromatic compounds by P. chrysosporium. The preference of PcMHQDs for MHQ and DMHQ not only highlights their potential for biotechnological applications but also underscores their critical role in understanding lignin degradation by a representative of white-rot fungus, P. chrysosporium.
• In vitro co-expression chromatin assembly and remodeling platform for plant histone variants.
  Petra Banko; Kei-Ichi Okimune; Szilvia K Nagy; Akinori Hamasaki; Ryo Morishita; Hitoshi Onouchi; Taichi E Takasuka
  Scientific reports, 14, 1, 936, 936, 10 Jan. 2024, [Peer-reviewed], [Last author, Corresponding author], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal, Histone variants play a central role in shaping the chromatin landscape in plants, yet, how their distinct combinations affect nucleosome properties and dynamics is still largely elusive. To address this, we developed a novel chromatin assembly platform for Arabidopsis thaliana, using wheat germ cell-free protein expression. Four canonical histones and five reported histone variants were used to assemble twelve A. thaliana nucleosome combinations. Seven combinations were successfully reconstituted and confirmed by supercoiling and micrococcal nuclease (MNase) assays. The effect of the remodeling function of the CHR11-DDR4 complex on these seven combinations was evaluated based on the nucleosome repeat length and nucleosome spacing index obtained from the MNase ladders. Overall, the current study provides a novel method to elucidate the formation and function of a diverse range of nucleosomes in plants.
• Quantitative analysis of The High Yield Hydrolysis of Kelp by Laminarinase and Alginate Lyase.
  Taichi Takasuka; Hoon Kim; Kai Deng; Christopher M Bianchetti; Kaho Yamashita; Emily T Beebe; Lai F Bergeman; Kirk A Vander Meulen; Samuel Deutsch; John Ralph; Paul D Adams; Trent R Northen; Brian G Fox
  Chembiochem : a European journal of chemical biology, e202300357, 04 Jul. 2023, [Peer-reviewed], [Lead author], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal, Kelp is an abundant, farmable biomass containing laminarin and alginate as major polysaccharides, providing an excellent model substrate for study of their deconstruction by simple enzyme mixtures. Our previous study showed strong reactivity of glycoside hydrolase family 55 during hydrolysis of purified laminarin, raising the question of its reactivity with intact kelp. In this study, we determined that combination of a single glycoside hydrolase family 55 β-1,3-exoglucanase with a broad specificity alginate lyase from polysaccharide lyase family 18 gives efficient hydrolysis of untreated kelp to a mixture of simple sugars glucose, gentiobiose, mannitol-end glucose, and mannuronic and guluronic acids and their soluble oligomers. Quantitative assignments from nanostructure initiator mass spectrometry (NIMS) and 2D HSQC NMR and analysis of the reaction time-course are provided. The data suggests that binary combinations of enzymes targeted to the unique polysaccharide composition of marine biomass are sufficient to deconstruct kelp into soluble sugars for microbial fermentation.
• Binding properties of recombinant LDL receptor and LOX-1 receptor to LDL measured using bio-layer interferometry and atomic force microscopy.
  Seiji Takeda; Ao Hamamuki; Kanako Ushirogata; Taichi E Takasuka
  Biophysical chemistry, 300, 107069, 107069, 14 Jun. 2023, [Peer-reviewed], [Last author], [International Magazine]
  English, Scientific journal, Oxidation of low-density lipoproteins (LDLs) triggers a recognition by scavenger receptors such as lectin-like oxidized LDL receptor-1 (LOX-1) and is related to inflammation and cardiovascular diseases. Although LDLs that are recognized by LOX-1 can be risk-related LDLs, conventional LDL detection methods using commercially available recombinant receptors remain undeveloped. Using a bio-layer interferometry (BLI), we investigated the binding of recombinant LOX-1 (reLOX-1) and LDL receptors to the oxidized LDLs. The recombinant LDL receptor preferably bound minimally modified LDLs, while the reLOX-1 recognized extensively oxidized LDLs. An inversed response of the BLI was observed during the binding in the case of reLOX-1. AFM study showed that the extensively oxidized LDLs and aggregates of LDLs were observed on the surface, supporting the results. Altogether, a combined use of these recombinant receptors and the BLI method is useful in detecting high-risk LDLs such as oxidized LDLs and modified LDLs.
• In Vitro Synthesis and Design of Kinesin Biomolecular Motors by Cell-Free Protein Synthesis.
  Daisuke Inoue; Keisuke Ohashi; Taichi E Takasuka; Akira Kakugo
  ACS synthetic biology, 23 May 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Kinesin is a biomolecular motor that generates force and motility along microtubule cytoskeletons in cells. Owing to their ability to manipulate cellular nanoscale components, microtubule/kinesin systems show great promise as actuators of nanodevices. However, classical in vivo protein production has some limitations for the design and production of kinesins. Designing and producing kinesins is laborious, and conventional protein production requires specific facilities to create and contain recombinant organisms. Here, we demonstrated the in vitro synthesis and editing of functional kinesins using a wheat germ cell-free protein synthesis system. The synthesized kinesins propelled microtubules on a kinesin-coated substrate and showed a higher binding affinity with microtubules than E. coli-produced kinesins. We also successfully incorporated affinity tags into the kinesins by extending the original sequence of the DNA template by PCR. Our method will accelerate the study of biomolecular motor systems and encourage their wider use in various nanotechnology applications.
• Consolidated bioprocessing of plant biomass to polyhydroxyalkanoate by co-culture of Streptomyces sp. SirexAA-E and Priestia megaterium.
  Vijay Kumar; Brian G Fox; Taichi E Takasuka
  Bioresource technology, 376, 128934, 128934, May 2023, [Peer-reviewed], [Last author, Corresponding author], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal, Polyhydroxyalkanoate (PHA) production from plant biomass is an ideal way to realize sustainable PHA-based bioplastic. The present study demonstrated consolidated bioconversion of plant biomass to PHA by co-culturing two specialized bacteria, cellulolytic Streptomyces sp. SirexAA-E and PHA producing Priestia megaterium. In monoculture, S. sp. SirexAA-E does not produce PHA, while P. megaterium did not grow on plant polysaccharides. The co-culture showed poly(3-hydroxybutyrate) (PHB) production using purified polysaccharides, including cellulose, xylan, mannan and their combinations, and plant biomass (Miscanthus, corn stalk and corn leaves) as sole carbon sources, confirmed by GC-MS. The co-culture inoculated with 1:4 (v/v) ratio of S. sp. SirexAA-E to P. megaterium produced 40 mg PHB/g Miscanthus using 0.5% biomass loading. Realtime PCR showed ∼85% S. sp. SirexAA-E and ∼15% P. megaterium in the co-culture. Thus, this study provides a concept of proof for one-pot bioconversion of plant biomass into PHB without separate saccharification processes.
• Genomic and Secretomic Analyses of the Newly Isolated Fungus Perenniporia fraxinea SS3 Identified CAZymes Potentially Related to a Serious Pathogenesis of Hardwood Trees.
  Ruy Matsumoto; Jakia Jerin Mehjabin; Hideki Noguchi; Toshizumi Miyamoto; Taichi E Takasuka; Chiaki Hori
  Applied and environmental microbiology, e0027223, 26 Apr. 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Perenniporia fraxinea can colonize living trees and cause severe damage to standing hardwoods by secreting a number of carbohydrate-activate enzymes (CAZymes), unlike other well-studied Polyporales. However, significant knowledge gaps exist in understanding the detailed mechanisms for this hardwood-pathogenic fungus. To address this issue, five monokaryotic P. fraxinea strains, SS1 to SS5, were isolated from the tree species Robinia pseudoacacia, and high polysaccharide-degrading activities and the fastest growth were found for P. fraxinea SS3 among the isolates. The whole genome of P. fraxinea SS3 was sequenced, and its unique CAZyme potential for tree pathogenicity was determined in comparison to the genomes of other nonpathogenic Polyporales. These CAZyme features are well conserved in a distantly related tree pathogen, Heterobasidion annosum. Furthermore, the carbon source-dependent CAZyme secretions of P. fraxinea SS3 and a nonpathogenic and strong white-rot Polyporales member, Phanerochaete chrysosporium RP78, were compared by activity measurements and proteomic analyses. As seen in the genome comparisons, P. fraxinea SS3 exhibited higher pectin-degrading activities and higher laccase activities than P. chrysosporium RP78, which were attributed to the secretion of abundant glycoside hydrolase family 28 (GH28) pectinases and auxiliary activity family 1_1 (AA1_1) laccases, respectively. These enzymes are possibly related to fungal invasion into the tree lumens and the detoxification of tree defense substances. Additionally, P. fraxinea SS3 showed secondary cell wall degradation capabilities at the same level as that of P. chrysosporium RP78. Overall, this study suggested mechanisms for how this fungus can attack the cell walls of living trees as a serious pathogen and differs from other nonpathogenic white-rot fungi. IMPORTANCE Many studies have been done to understand the mechanisms underlying the degradation of plant cell walls of dead trees by wood decay fungi. However, little is known about how some of these fungi weaken living trees as pathogens. P. fraxinea belongs to the Polyporales, a group of strong wood decayers, and is known to aggressively attack and fell standing hardwood trees all over the world. Here, we report CAZymes potentially related to plant cell wall degradation and pathogenesis factors in a newly isolated fungus, P. fraxinea SS3, by genome sequencing in conjunction with comparative genomic and secretomic analyses. The present study provides insights into the mechanisms of the degradation of standing hardwood trees by the tree pathogen, which will contribute to the prevention of this serious tree disease.
• Biochemical characterization of plant aromatic aminotransferases
  Koper, K; Hataya, S; Hall, A.G; Takasuka, T.E; Maeda, H.A
  Methods Enzymol., Dec. 2022, [Peer-reviewed], [Invited], ［Internationally co-authored］, [International Magazine]
  English
• AtTrxh3, a Thioredoxin, Is Identified as an Abscisic AcidBinding Protein in Arabidopsis thaliana
  Tomoaki Anabuki; Keisuke Ohashi; Taichi E. Takasuka; Hideyuki Matsuura; Kosaku Takahashi
  Molecules, 27, 1, 161, 161, MDPI AG, 28 Dec. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Abscisic acid (ABA, 1) is a plant hormone that regulates various plant physiological processes such as seed developing and stress responses. The ABA signaling system has been elucidated; binding of ABA with PYL proteins triggers ABA signaling. We have previously reported a new method to isolate a protein targeted with a bioactive small molecule using a biotin linker with alkyne and amino groups, a protein cross-linker, and a bioactive small molecule with an azido group (azido probe). This method was used to identify the unknown ABA binding protein of Arabidopsis thaliana. As a result, AtTrxh3, a thioredoxin, was isolated as an ABA binding protein. Our developed method can be applied to the identification of binding proteins of bioactive compounds.
• Histone chaperone‐mediated co‐expression assembly of tetrasomes and nucleosomes
  Kei‐ichi Okimune; Shogo Hataya; Kazuki Matsumoto; Kanako Ushirogata; Petra Banko; Seiji Takeda; Taichi E. Takasuka
  FEBS Open Bio, Wiley, 06 Oct. 2021, [Peer-reviewed], [Last author, Corresponding author], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal
• Mannose- and Mannobiose-Specific Responses of the Insect-Associated Cellulolytic Bacterium Streptomyces sp. Strain SirexAA-E
  Keisuke Ohashi; Shogo Hataya; Akane Nakata; Kazuki Matsumoto; Natsumi Kato; Wakana Sato; Camila Carlos-Shanley; Emily T. Beebe; Cameron R. Currie; Brian G. Fox; Taichi E. Takasuka
  Applied and Environmental Microbiology, 87, 14, American Society for Microbiology, 25 Jun. 2021, [Peer-reviewed], [Last author, Corresponding author], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal, Streptomyces
  sp. SirexAA-E, a microbial symbiont of biomass-harvesting insects, secretes a suite of polysaccharide-degrading enzymes dependent on the available carbon sources., 29716061
• De novo reconstitution of chromatin using wheat germ cell‐free protein synthesis
  Yaeta Endo; Nobuaki Takemori; Szilvia K. Nagy; Kei‐ichi Okimune; Rohinton Kamakaka; Hitoshi Onouchi; Taichi E. Takasuka
  FEBS Open Bio, 11, 6, 1552, 1564, Wiley, Jun. 2021, [Peer-reviewed], [Last author, Corresponding author], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal, DNA is packaged with histones to form chromatin that impinges on all nuclear processes, including transcription, replication and repair, in the eukaryotic nucleus. A complete understanding of these molecular processes requires analysis of chromatin context in vitro. Here, Drosophila four core histones were produced in a native and unmodified form using wheat germ cell-free protein synthesis. In the assembly reaction, four unpurified core histones and three chromatin assembly factors (dNAP-1, dAcf1 and dISWI) were incubated with template DNA. We then assessed stoichiometry with the histones, nucleosome arrays, supercoiling and the ability of the chromatin to serve as a substrate for histone-modifying enzymes. Overall, our method provides a new avenue to produce chromatin that can be useful in a wide range of chromatin research.
• Transport-coupled ubiquitination of the borate transporter BOR1 for its boron-dependent degradation
  Akira Yoshinari; Takuya Hosokawa; Marcel Pascal Beier; Keishi Oshima; Yuka Ogino; Chiaki Hori; Taichi E Takasuka; Yoichiro Fukao; Toru Fujiwara; Junpei Takano
  The Plant Cell, 33, 2, 420, 438, Oxford University Press ({OUP}), 03 Dec. 2020, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal,
  Plants take up and translocate nutrients through transporters. In Arabidopsis thaliana, the borate exporter BOR1 acts as a key transporter under boron (B) limitation in the soil. Upon sufficient-B supply, BOR1 undergoes ubiquitination and is transported to the vacuole for degradation, to avoid overaccumulation of B. However, the mechanisms underlying B-sensing and ubiquitination of BOR1 are unknown. In this study, we confirmed the lysine-590 residue in the C-terminal cytosolic region of BOR1 as the direct ubiquitination site and showed that BOR1 undergoes K63-linked polyubiquitination. A forward genetic screen identified that amino acid residues located in vicinity of the substrate-binding pocket of BOR1 are essential for the vacuolar sorting. BOR1 variants that lack B-transport activity showed a significant reduction of polyubiquitination and subsequent vacuolar sorting. Coexpression of wild-type (WT) and a transport-defective variant of BOR1 in the same cells showed degradation of the WT but not the variant upon sufficient-B supply. These findings suggest that polyubiquitination of BOR1 relies on its conformational transition during the transport cycle. We propose a model in which BOR1, as a B transceptor, directly senses the B concentration and promotes its own polyubiquitination and vacuolar sorting for quick and precise maintenance of B homeostasis.
• Reconstitution of Drosophila and human chromatins by wheat germ cell-free co-expression system.
  Kei-Ichi Okimune; Szilvia K Nagy; Shogo Hataya; Yaeta Endo; Taichi E Takasuka
  BMC biotechnology, 20, 1, 62, 62, 01 Dec. 2020, [Peer-reviewed], [Last author, Corresponding author], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal, BACKGROUND: Elaboration of the epigenetic regulation of chromatin is a long-standing aim in molecular and cellular biology. Hence, there is a great demand for the development of in vitro methods to reconstitute chromatin that can be used directly for biochemical assays. The widely used wheat germ cell-free protein expression method provides broad applications to investigate the function and structure of eukaryotic proteins. Such advantages, including high translation efficiency, flexibility, and possible automatization, are beneficial for achieving native-like chromatin substrates for in vitro studies. RESULTS: We describe a novel, single-step in vitro chromatin assembly method by using the wheat germ cell-free protein synthesis. We demonstrated that both Drosophila and human chromatins can be reconstituted in the course of the in vitro translation of core histones by the addition of chromatin assembly factors, circular plasmid, and topoisomerase I in an ATP-dependent manner. Drosophila chromatin assembly was performed in 4 h at 26 °C, in the presence of premixed mRNAs encoding the core histones, dAcf1/dISWI chromatin remodeling complex, and nucleosome assembly protein, dNAP1. Similarly, the human chromatin was assembled by co-expressing the human core histones with Drosophila chromatin remodeling factor, dISWI, and chromatin chaperone, dNLP, for 6 h at 26 °C. The presence of reconstituted chromatin was monitored by DNA supercoiling assay, also the regular spacing of nucleosomes was assessed by Micrococcal nuclease assay. Furthermore, Drosophila linker histone H1-containing chromatin was reconstituted, affirming that the in vitro assembled chromatin is suitable for downstream applications. CONCLUSIONS: The method described in this study allows the assembly of Drosophila and human chromatins, possibly in native-like form, by using a wheat germ cell-free protein expression. Although both chromatins were reconstituted successfully, there were unexpected differences with respect to the required ratio of histone-coding mRNAs and the reaction time. Overall, our new in vitro chromatin reconstitution method will aid to characterize the unrevealed structure, function, and regulation of chromatin dynamics.
• A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis.
  Evan M Glasgow; Elias I Kemna; Craig A Bingman; Nicole L Ing; Kai Deng; Christopher M Bianchetti; Taichi E Takasuka; Trent R Northen; Brian G Fox
  The Journal of biological chemistry, 16 Oct. 2020, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal, Broad-specificity glycoside hydrolases (GHs) contribute to plant biomass hydrolysis by degrading a diverse range of polysaccharides, making them useful catalysts for renewable energy and biocommodity production. Discovery of new GHs with improved kinetic parameters or more tolerant substrate binding sites could increase the efficiency of renewable bioenergy production even further. GH5 has over 50 subfamilies exhibiting selectivities for reaction with β-(1,4)-linked oligo- and polysaccharides. Among these, subfamily 4 (GH5_4) contains numerous broad-selectivity endoglucanases that hydrolyze cellulose, xyloglucan, and mixed-linkage glucans. We previously surveyed the whole subfamily and found over 100 new broad-specificity endoglucanases, although the structural origins of broad specificity remained unclear. A mechanistic understanding of GH5_4 substrate specificity would help inform the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases. Here we report structures of ten new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and mass spectrometry. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan. The positions of key aromatic residues determine the overall reaction rate and breadth of substrate tolerance, and they contribute to differences in oligosaccharide cleavage patterns. Our new composite model identifies several critical structural features that confer broad specificity and may be readily engineered into existing industrial enzymes. We demonstrate that GH5_4 endoglucanases can have broad specificity without sacrificing high activity, making them a valuable addition to the biomass deconstruction toolset.
• Proteomic characterization of lignocellulolytic enzymes secreted by the insect-associated fungus, Daldinia decipiens oita, isolated from the forest in northern Japan.
  Chiaki Hori; Ruopu Song; Kazuki Matsumoto; Ruy Matsumoto; Benjamin B Minkoff; Shuzo Oita; Hideho Hara; Taichi E Takasuka
  Applied and environmental microbiology, 14 Feb. 2020, [Peer-reviewed], [Last author, Corresponding author], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal, Wood-devastating insects utilize their symbiotic microbes with lignocellulose-degrading abilities to extract energy from recalcitrant woods. It is well known that free-living lignocellulose-degrading fungi secrete various carbohydrate-active enzymes (CAZymes) to degrade plant cell wall components, mainly cellulose, hemicellulose and lignin. However, CAZymes from insect-symbiotic fungi have not been well documented except for a few examples. In this study, an insect-associated fungus, Daldinia decipiens oita was isolated as a potential symbiotic fungus of female Xiphydriaalbopicta captured from Hokkaido forest. This fungus was grown in seven different media containing a single carbon source, glucose, cellulose, xylan, mannan, pectin, poplar, or larch, and the secreted proteins were identified by LC-MS/MS. 128 CAZymes including domains of 92 glycoside hydrolases (GHs), 15 carbohydrate esterases (CEs), 5 polysaccharide lyases (PLs), 17 auxiliary activities (AAs), and 11 carbohydrate-binding modules (CBMs) were identified, and these are involved in degradation of cellulose and hemicellulose but not lignin. Together with the result of polysaccharide-degrading activity measurements, we concluded that D. decipiens oita tightly regulates the expression of these CAZymes in response to the tested plant cell wall materials. Overall, this study described the detailed proteomic approach of a woodwasp-associated fungus and revealed that the new isolate, D. decipiens oita, secretes diverse CAZymes to efficiently degrade lignocellulose in the symbiotic environment.ImportanceRecent studies show the potential impacts of insect symbiont microbes on biofuels application with regards to their degradation capability of a recalcitrant plant cell wall. In this study, we describe a novel fungal isolate, D. decipiens oita, as a single symbiotic fungus from the Xiphydria woodwasp found in northern forests of Japan. Our detailed secretome analyses of D. decipiens oita together with activity measurements reveal that this insect-associated fungus exhibits high and broad activities for plant cell wall material degradation, suggesting potential applications within the biomass conversion industry for plant mass degradation.
• Identification of recombinant AtPYL2, an abscisic acid receptor, in E. coli using a substrate-derived bioactive small molecule, a biotin linker with alkyne and amino groups, and a protein cross-linker.
  Tomoaki Anabuki; Yusuke Ito; Keisuke Ohashi; Taichi E Takasuka; Hideyuki Matsuura; Kosaku Takahashi
  Bioorganic & medicinal chemistry letters, 29, 21, 126634, 126634, 01 Nov. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Target protein identification of bioactive small molecules is one of the most important research in forward chemical genetics. The affinity chromatography technique to use a resin bound with a small molecule is often used for identification of a target protein of a bioactive small molecule. Here we report a new method to isolate a protein targeted with a bioactive small molecule using a biotin linker with alkyne and amino groups, protein cross-linker containing disulfide bond, and a bioactive small molecule with an azido group (azido probe). After an azido probe is associated with a target protein, the complex of a target protein and azido probe is covalently bound through the biotin linker by azide-alkyne Huisgen cycloaddition and protein cross-linker containing disulfide bond. This ternary complex is immobilized on an affinity matrix with streptavidin, and then the target protein is selectively eluted with a buffer containing a reducing agent for cleavage of disulfide bonds. This method uses a probe having an azido group, which a small functional group, and has the possibility to be a solution strategy to overcome the hindrance of a functional group introduced into the probe that reduces association a target protein. The effectiveness of the method in this study was shown using linker 1, 3'-azidoabscisic acid 3, and protein cross-linker containing a disulfide bond (DTSSP 5).
• Antarctic heterotrophic bacterium Hymenobacter nivis P3(T) displays light-enhanced growth and expresses putative photoactive proteins
  Mia Terashima; Keisuke Ohashi; Taichi E. Takasuka; Hisaya Kojima; Manabu Fukui
  ENVIRONMENTAL MICROBIOLOGY REPORTS, 11, 2, 227, 235, Apr. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Genomes of Neutrophilic Sulfur-Oxidizing Chemolithoautotrophs Representing 9 Proteobacterial Species From 8 Genera
  Tomohiro Watanabe; Hisaya Kojima; Kazuhiro Umezawa; Chiaki Hori; Taichi E. Takasuka; Yukako Kato; Manabu Fukui
  FRONTIERS IN MICROBIOLOGY, 10, 316, Feb. 2019, [Peer-reviewed]
  English, Scientific journal
• Extent and Origins of Functional Diversity in a Subfamily of Glycoside Hydrolases.
  Glasgow EM; Vander Meulen KA; Takasuka TE; Bianchetti CM; Bergeman LF; Deutsch S; Fox BG
  Journal of molecular biology, Jan. 2019, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal
• Towards Better Problem Finding and Creativity in Graduate School Education: A Case Study of Nitobe School Program
  Ankit Ravankar; Shotaro Imai; Abhijeet Ravankar; Tomohiro Agatsuma; Tomomichi Kato; Taichi Takasuka; Teruyuki Tsuji; Kaori K. Shigetomi; Ken Saito
  Proceedings - 2018 7th International Congress on Advanced Applied Informatics, IIAI-AAI 2018, 414, 418, 02 Jul. 2018, [Peer-reviewed]
  English, Scientific journal
• Optimization of simultaneous saccharification and fermentation conditions with amphipathic lignin derivatives for concentrated bioethanol production
  Ningning Cheng; Keiichi Koda; Yutaka Tamai; Yoko Yamamoto; Taichi E. Takasuka; Yasumitsu Uraki
  BIORESOURCE TECHNOLOGY, 232, 126, 132, May 2017, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal
• Effect of instructor's actions and attitudes on student's motivation and discussion process in TBL class for graduate students.
  Imai S; Ravankar RA; Shimamura M; Takasuka TE; Chiba G; Yamanaka Y
  International Journal of Institutional Research and Management., 1, 17, 35, Apr. 2017, [Peer-reviewed]
  English, Scientific journal
• Problem-based learning and problem finding among university graduate students.
  Ravankar RA; Imai S; Shiamura M; Chiba G; Takasuka TE
  J Higher Education and Lifelong Learning, 24, 24, 9, 20, 北海道大学高等教育推進機構, Apr. 2017, [Peer-reviewed]
  English, In recent years, problem-based learning (PBL) techniques have been gaining momentum inschools and university curricula around the world. The main advantage of the PBL method is that it promotescreative problem solving, improves cognition and enhances overall thought processes in learners. For mostPBL-style programmes, problem solving is at the core, although the notion of problem discovery or problemfinding is not seriously considered. In most cases, students are always presented with a structured and welldefinedproblem, but have no experience of solving an ill-structured problem or ʻwicked' problem. Thepresent study focuses on problem finding as a critical step towards developing problem solving skills inuniversity graduate students. The study aims at understanding the importance of problem formulation andcreativity, and focuses as well on our attempt to teach problem finding as an important tool in thedevelopment of creative thinking and problem solving among graduate students. The study is part of a specialgraduate programme called the Nitobe School at Hokkaido University in Japan, which started in 2015. In anactive learning classroom setting, this course is intended to support graduate students in their discovery of illstructuredproblems, help them to understand their formulation and thereby improve their problem solvingskills. We present the results of our teaching method for the first year at the Nitobe School and share ourfindings through this work.
• Evolution of High Cellulolytic Activity in Symbiotic Streptomyces through Selection of Expanded Gene Content and Coordinated Gene Expression
  Adam J. Book; Gina R. Lewin; Bradon R. McDonald; Taichi E. Takasuka; Evelyn Wendt-Pienkowski; Drew T. Doering; Steven Suh; Kenneth F. Raffa; Brian G. Fox; Cameron R. Currie
  PLOS BIOLOGY, 14, 6, Jun. 2016, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal
• ,
  Taichi TAKASUKA; Chiaki HORI; James ELLINGER; Yuki TOBIMATSU
  化学と生物, 54, 3, 156, 158, Japan Society for Bioscience, Biotechnology, and Agrochemistry, 2016, [Peer-reviewed]
  Japanese, Scientific journal
• Discussion on a method of Team-Based-Learning style lecture for graduate students in a research university
  Shotaro Imai; Ankit A. Ravankar; Michiyo Shimamura; Taichi E. Takasuka; Go Chiba; Yasuhiro Yamanaka
  PROCEEDINGS 2016 5TH IIAI INTERNATIONAL CONGRESS ON ADVANCED APPLIED INFORMATICS IIAI-AAI 2016, 537, 541, 2016, [Peer-reviewed]
  English, International conference proceedings
• Nurturing Problem-Finding Skills in Graduate Students through Problem Based Learning Approaches
  Ankit A. Ravankar; Shotaro Imai; Michiyo Shimamura; Go Chiba; Taichi Takasuka; Yasuhiro Yamanaka
  PROCEEDINGS 2016 5TH IIAI INTERNATIONAL CONGRESS ON ADVANCED APPLIED INFORMATICS IIAI-AAI 2016, 542, 546, 2016, [Peer-reviewed]
  English, International conference proceedings
• Multifunctional cellulase catalysis targeted by fusion to different carbohydrate-binding modules
  Johnnie A. Walker; Taichi E. Takasuka; Kai Deng; Christopher M. Bianchetti; Hannah S. Udell; Ben M. Prom; Hyunkee Kim; Paul D. Adams; Trent R. Northen; Brian G. Fox
  BIOTECHNOLOGY FOR BIOFUELS, 8, 220, Dec. 2015, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Active Site and Laminarin Binding in Glycoside Hydrolase Family 55
  Christopher M. Bianchetti; Taichi E. Takasuka; Sam Deutsch; Hannah S. Udell; Eric J. Yik; Lai F. Bergeman; Brian G. Fox
  JOURNAL OF BIOLOGICAL CHEMISTRY, 290, 19, 11819, 11832, May 2015, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Use of Nanostructure-Initiator Mass Spectrometry to Deduce Selectivity of Reaction in Glycoside Hydrolases.
  Deng K; Takasuka TE; Bianchetti CM; Bergeman LF; Adams PD; Northen TR; Fox BG
  Frontiers in bioengineering and biotechnology, 3, 165, 2015, [Peer-reviewed]
  English, Scientific journal
• Development of a High Throughput Platform for Screening Glycoside Hydrolases Based on Oxime-NIMS.
  Deng K; Guenther JM; Gao J; Bowen BP; Tran H; Reyes-Ortiz V; Cheng X; Sathitsuksanoh N; Heins R; Takasuka TE; Bergeman LF; Geertz-Hansen H; Deutsch S; Loqué D; Sale KL; Simmons BA; Adams PD; Singh AK; Fox BG; Northen TR
  Frontiers in bioengineering and biotechnology, 3, 153, 2015, [Peer-reviewed]
  English, Scientific journal
• Cellulolytic Streptomyces Strains Associated with Herbivorous Insects Share a Phylogenetically Linked Capacity To Degrade Lignocellulose
  Adam J. Book; Gina R. Lewin; Bradon R. McDonald; Taichi E. Takasuka; Drew T. Doering; Aaron S. Adams; Joshua A. V. Blodgett; Jon Clardy; Kenneth F. Raffa; Brian G. Fox; Cameron R. Currie
  APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80, 15, 4692, 4701, Aug. 2014, [Peer-reviewed]
  English, Scientific journal
• Evolution of substrate specificity in bacterial AA10 lytic polysaccharide monooxygenases
  Adam J. Book; Ragothaman M. Yennamalli; Taichi E. Takasuka; Cameron R. Currie; George N. Phillips; Brian G. Fox
  BIOTECHNOLOGY FOR BIOFUELS, 7, 109, Aug. 2014, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Structure-guided analysis of catalytic specificity of the abundantly secreted chitosanase SACTE_ 5457 from Streptomyces sp SirexAA-E
  Taichi E. Takasuka; Christopher M. Bianchetti; Yuki Tobimatsu; Lai F. Bergeman; John Ralph; Brian G. Fox
  PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 82, 7, 1245, 1257, Jul. 2014, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Rapid Kinetic Characterization of Glycosyl Hydrolases Based on Oxime Derivatization and Nanostructure-Initiator Mass Spectrometry (NIMS)
  Kai Deng; Taichi E. Takasuka; Richard Heins; Xiaoliang Cheng; Lai F. Bergeman; Jian Shi; Ryan Aschenbrener; Sam Deutsch; Seema Singh; Kenneth L. Sale; Blake A. Simmons; Paul D. Adams; Anup K. Singh; Brian G. Fox; Trent R. Northen
  ACS CHEMICAL BIOLOGY, 9, 7, 1470, 1479, Jul. 2014, [Peer-reviewed]
  English, Scientific journal
• Biochemical Properties and Atomic Resolution Structure of a Proteolytically Processed beta-Mannanase from Cellulolytic Streptomyces sp SirexAA- E
  Taichi E. Takasuka; Justin F. Acheson; Christopher M. Bianchetti; Ben M. Prom; Lai F. Bergeman; Adam J. Book; Cameron R. Currie; Brian G. Fox
  PLOS ONE, 9, 4, e94166, Apr. 2014, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Miniaturized Sequencing Gel System for Quick Analysis of DNA by Hydroxyl Radical Cleavage
  Taichi E. Takasuka; Yi-Ju Hsieh; Arnold Stein
  APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 172, 1, 1, 8, Jan. 2014, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Cell-free translation of biofuel enzymes.
  Takasuka TE; Walker JA; Bergeman LF; Vander Meulen KA; Makino S; Elsen NL; Fox BG
  Methods in molecular biology (Clifton, N.J.), 1118, 71, 95, 2014, [Peer-reviewed], [Lead author]
• Fusion of Dioxygenase and Lignin-binding Domains in a Novel Secreted Enzyme from Cellulolytic Streptomyces sp SirexAA-E
  Christopher M. Bianchetti; Connor H. Harmann; Taichi E. Takasuka; Gregory L. Hura; Kevin Dyer; Brian G. Fox
  JOURNAL OF BIOLOGICAL CHEMISTRY, 288, 25, 18574, 18587, Jun. 2013, [Peer-reviewed]
  English, Scientific journal
• Aerobic deconstruction of cellulosic biomass by an insect-associated Streptomyces.
  Takasuka TE; Book AJ; Lewin GR; Currie CR; Fox BG
  Scientific reports, 3, 1030, 2013, [Peer-reviewed], [Lead author]
• A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres
  Aarthi Chandrasekaran; Kai Deng; Chung-Yan Koh; Taichi Takasuka; Lai F. Bergeman; Brian G. Fox; Paul D. Adams; Anup K. Singh
  CHEMICAL COMMUNICATIONS, 49, 48, 5441, 5443, 2013, [Peer-reviewed]
  English, Scientific journal
• Global Gene Expression Patterns in Clostridium thermocellum as Determined by Microarray Analysis of Chemostat Cultures on Cellulose or Cellobiose
  Allison Riederer; Taichi E. Takasuka; Shin-ichi Makino; David M. Stevenson; Yury V. Bukhman; Nathaniel L. Elsen; Brian G. Fox
  APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77, 4, 1243, 1253, Feb. 2011, [Peer-reviewed]
  English, Scientific journal
• Proliferating Cell Nuclear Antigen (PCNA) Is Required for Cell Cycle-regulated Silent Chromatin on Replicated and Nonreplicated Genes
  Andrew Miller; Jiji Chen; Taichi E. Takasuka; Jennifer L. Jacobi; Paul D. Kaufman; Joseph M. K. Irudayaraj; Ann L. Kirchmaier
  JOURNAL OF BIOLOGICAL CHEMISTRY, 285, 45, 35142, 35154, Nov. 2010, [Peer-reviewed]
  English, Scientific journal
• Direct measurements of the nucleosome-forming preferences of periodic DNA motifs challenge established models
  Taichi E. Takasuka; Arnold Stein
  NUCLEIC ACIDS RESEARCH, 38, 17, 5672, 5680, Sep. 2010, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Are nucleosome positions in vivo primarily determined by histone-DNA sequence preferences?
  Arnold Stein; Taichi E. Takasuka; Clayton K. Collings
  NUCLEIC ACIDS RESEARCH, 38, 3, 709, 719, Jan. 2010, [Peer-reviewed]
  English, Scientific journal
• Sequence Information Encoded in DNA that May Influence Long-Range Chromatin Structure Correlates with Human Chromosome Functions
  Taichi E. Takasuka; Alfred Cioffi; Arnold Stein
  PLOS ONE, 3, 7, e2643, Jul. 2008, [Peer-reviewed], [Lead author]
  English, Scientific journal

• Phosphorylation And Dephosphorylation Regulate Polar Localization Of A Borate Transporter AtBOR1
  吉田麟太郎; 室啓太; 清水優大; 大橋慧介; 荻野由香; 堀千明; 笠井光治; 高須賀太一; 藤原徹; 高野順平; 高野順平, 日本植物生理学会年会(Web), 65th, 2024
• シロイヌナズナの根におけるホウ酸トランスポーターBOR1の細胞内偏在機構の解明
  吉田麟太郎; 室啓太; 清水優大; 大橋慧介; 荻野由香; 堀千明; 笠井光治; 高須賀太一; 藤原徹; 高野順平; 高野順平, 日本土壌肥料学会講演要旨集(Web), 70, 2024
• Novel rhamnogalacturonan lyase from Aspergillus nidulans: Structure and physiological roles
  鈴木裕満; 亀山綾音; 高須賀太一; 堀千明; 加藤雅士; 志水元亨, 糸状菌分子生物学コンファレンス(Web), 22nd, 2023
• Human chromatin reconstitution by cell-free translation for epigenetic modifier enzyme assays
  SK Nagy; P Banko; A Tar; K Bendali; T Meszaros; TE Takasuka, FEBS Open Bio 11 (Suppl. 1) DOI: 10.1002/2211-5463.13206, Jul. 2021
• Functional analysis of a novel rhamnogalacturonan lyase derived from Aspergillus nidulans
  伊東昂希; 鈴木裕満; 鈴木健吾; 酒井杏匠; 高須賀太一; 堀千明; 加藤雅士; 志水元亨, 日本農芸化学会大会講演要旨集(Web), 2021, 2021
• Functional and structural analysis of novel rhamnogalacturonan lyase from Aspergillus nidulans
  鈴木裕満; 伊東昂希; 酒井杏匠; 堀千明; 高須賀太一; 加藤雅士; 志水元亨, 日本生物工学会大会講演要旨集, 73rd, 2021
• A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis
  Evan M. Glasgow; Elias I. Kemna; Craig A. Bingman; Nicole Ing; Kai Deng; Christopher M. Bianchetti; Taichi E. Takasuka; Trent R. Northen; Brian G. Fox, Journal of Biological Chemistry, 295, 51, 17752, 17769, 18 Dec. 2020
  American Society for Biochemistry and Molecular Biology Inc., English, Book review
• 糸状菌Aspergillus nidulansのセクレトーム解析から同定されたhypothetical proteinの機能解析
  大堀沙貴子; 酒井杏匠; 糀谷紗季; 山口愛彩; 木島尚輝; 松江渚; 小栗莉奈; 高須賀太一; 堀千明; 志水元亨; 加藤雅士, 日本農芸化学会大会講演要旨集(Web), 2019, 2019
• 糸状菌Aspergillus nidulansが種々の多糖応答的に生産する細胞外酵素の網羅的解析
  山口 愛彩; 酒井 杏匠; 糀谷 紗季; 木島 尚輝; 大堀 紗貴子; 小栗 莉奈; 高須賀 太一; 堀 千明; 志水 元亨; 加藤 雅士, 日本生物工学会大会講演要旨集, 平成30年度, 166, 166, Aug. 2018
  (公社)日本生物工学会, Japanese
• Aspergillus nidulansのセクレトーム解析から見出された新規ガラクタン分解酵素
  鈴木裕満; 酒井杏匠; 新沢祐大; 鈴木健吾; 糀谷紗季; 山口愛彩; 松江渚; 高須賀太一; 堀千明; 志水元亨; 加藤雅士, 日本農芸化学会大会講演要旨集(Web), 2018, 2018
• Aspergillus nidulansのセクレトーム解析から見出された新規rhamnogalacturonan lyase
  鈴木裕満; 酒井杏匠; 鈴木健吾; 山口愛彩; 高須賀太一; 堀千明; 志水元亨; 加藤雅士, 日本農芸化学会中部支部例会講演要旨集(Web), 183rd, 2018
• Substrate Binding Effects of Carbohydrate Binding Modules on the Catalytic Activity of a Multifunctional Cellulase
  Johnnie A. Walker; Taichi E. Takasuka; Kai Deng; Christopher M. Bianchetti; Hannah Udell; Ben M. Prom; Hyunkee Kim; Trent R. Northern; Brian G. Fox, BIOPHYSICAL JOURNAL, 108, 2, 225A, 225A, Jan. 2015
  English, Summary international conference

• From north researchers to south researchers
  Taichi Takasuka
  seikagaku, Dec. 2019, [Others]
• Discovery of the novel insect-symbiont cellulolytic microbes and biochemical analyses of their enzymes to enhance polysaccharide deconstruction technology.
  Keisuke Ohashi; Chiaki Hori; Takasuka Taichi, Corresponding author
  seikagaku, May 2017, [Joint work]
• Lignocellulose degradation by insect-symbiont microbes
  Takasuka Taichi; Chiaki Hori; James Ellinger; Yuki Tobimatsu, Corresponding author
  kagakutoseibutsu, Feb. 2016, [Joint work]

• Secretome profiling of newly isolated polysaccharide-degrading bacillus species
  Saki Mitsumasu; Yu Kasuga; Tatsuya Nagano; Vijay Kumar; Yoshinori Hasegawa; Tomoya Maeda; Taichi Takasuka
  The 2026 Annual Meeting of JSBBA., 12 Mar. 2026, Japanese, Oral presentation
  09 Mar. 2026 - 12 Mar. 2026, 50531818
• Biotechnological approaches for sustainable bioeconomy: Integrating microbial engineering with green and blue carbon strategies
  Taichi Takasuka
  Pacifichem 2025, 15 Dec. 2025, English, Invited oral presentation
  15 Dec. 2025 - 20 Dec. 2025, 48482550;51331192;51304049, [Invited]
• Cellulolytic Streptomyces and potential application in biomaterial production from plant and kelp biomass.
  Taichi Takasuka
  Department of Biochemistry and Wisconsin Energy Institute, University of Wisconsin-Madison, 17 Sep. 2025, English
  48482550;50531818;42097727, [Invited]
• Chromatin structural and functional analyses using in vitro cell-free chromatin assembly method
  Taichi Takasuka
  Department of Biochemistry, Purdue University, West Lafayette, 12 Sep. 2025, English, Public discourse
  50198340, [Invited]
• Proteomic analysis of intracellular response of a highly cellulolytic Streptomyces sp. SirexAA-E to xylooligosaccharides
  Nagano Tatsuya; Ohashi Keisuke; Banko Petra; Kumar Vijay; Hori Chiaki; Fox Brian; Takasuka Taichi
  20th International Symposium on the Biology of Actinomycetes, The Netherlands, 17 Jun. 2025, English, Poster presentation
  15 Jun. 2025 - 19 Jun. 2025, 42097727
• Comprehensive functional characterization of aminotransferases for the construction of Nitrogen Metabolic Pathway in E. coli.
  Shyogo Hataya; Sora Fukui; Kaan Koper; Sebastian Huss; Zoran Nikoloski; Hiroshi Maeda; Taichi Takasuka
  MBSJ 47th, 29 Nov. 2024, English, Poster presentation
  27 Nov. 2024 - 29 Nov. 2024
• Enhanced poly(3-hydroxybutyrate) production from cellobiose by genome engineered Priestia megaterium using CRISPR-Cas9 technology
  Tatsuya Nagano; Vijay Kumar; Taichi Takasuka
  MBSJ 47th, 29 Nov. 2024, English, Poster presentation
  27 Nov. 2024 - 29 Nov. 2024
• Biochemical elucidation of novel bi-function in a xylan-responsive transcriptional regulator in Streptomyces sp. SirexAA-E
  Naoki Hayashi; Keisuke Ohashi; Tatsuya Nagano; Keiichi Okimune; Taichi Takasuka
  MBSJ 47th, 29 Nov. 2024, English, Poster presentation
  27 Nov. 2024 - 29 Nov. 2024
• Study of metal ion dependence function of PL17 family enzyme
  Sora Fukui; Brian Fox; Taichi Takasuka
  MBSJ 47th, 29 Nov. 2024, English, Poster presentation
  27 Nov. 2024 - 29 Nov. 2024
• Single particle analysis of nucleosomes assembled by wheat germ cell-free co-expression system
  Keiichi Okimune; Taiki Azuma; Junko Haga; Petra Banko; Ryo Morishita; Shunsuke Kita; Katsumi Maenaka; Taichi Takasuka
  MBSJ 47th, 28 Nov. 2024, English
  27 Nov. 2024 - 29 Nov. 2024
• Assessment of histone chaperone activitiesin plant H3-H4 octasome formation
  Junko Haga; Petra Banko; Keiichi Okimune; Taiki Azuma; Taichi Takasuka
  MBSJ 47th, 28 Nov. 2024, English, Poster presentation
  27 Nov. 2024 - 29 Nov. 2024
• Study of human H3-H4 octasome reconstitution with related chaperones in wheat germ cell-free co-expression platform
  Taiki Azuma; Keiichi Okimune; Junko Haga; Petra Banko; Taichi Takasuka
  MBSJ 47th, 28 Nov. 2024, English, Poster presentation
  27 Nov. 2024 - 29 Nov. 2024
• Isolation and omics analyses of cellulolytic microbes from Tomakomai Forest
  Mitsumasu Saki; Hayash Naoki; Nagano Tatsuya; Hasegawa Yoshinori; Takasuka Taichi
  MBSJ 47th, 27 Nov. 2024, English, Poster presentation
  27 Nov. 2024 - 29 Nov. 2024
• Study of human chromatin in vitro
  Taiki Azuma; Taichi Takasuka
  International Fisheries Symposium 2024, 21 Nov. 2024, English, Poster presentation
  20 Nov. 2024 - 21 Nov. 2024
• Assessment of histone chaperone activity
  Junko Haga; Taichi Takasuka
  International Fisheries Symposium 2024, 21 Nov. 2024, English, Poster presentation
  20 Nov. 2024 - 21 Nov. 2024
• Xylan-induced glyoxylate pathway in cellulolytic Streptomyces
  Tatsuya Nagano; Vijay Kumar; Taichi Takasuka
  Annual meeting for JSBBA, 27 Mar. 2024, Japanese, Oral presentation
  24 Mar. 2024 - 27 Mar. 2024, 31001902;29716061
• Minimal enzymatic combination to extract intact fucoidans from marine biomass.
  Sora Fukui; Taichi Takasuka
  Annual meeting for JSBBA, 25 Mar. 2024, Japanese, Oral presentation
  24 Mar. 2024 - 27 Mar. 2024, 29716010
• Genome engineering of Priestia megaterium for improved polyhydroxyalkanoate production from cellulosic-biomass in a co-culture system with cellulolytic Streptomyces sp. SirexAA-E
  Vijay Kumar; Taichi Takasuka
  The Molecular Biology Society of Japan, 08 Dec. 2023, English, Poster presentation
  06 Dec. 2023 - 08 Dec. 2023, 31001902;42097727
• Establishment of a method for extracting useful monosaccharides and natural fucoidan from algal biomass using multiple brown algae degrading enzymes
  Sora Fukui; Taichi Takasuka; Masashi Hosokawa; Hideyuki Kurihara; Shota Atsumi
  The Molecular Biology Society of Japan, 08 Dec. 2023, English, Poster presentation
  06 Dec. 2023 - 08 Dec. 2023
• Reconstitution of functional plant H3-H4 octasomes
  Junko Haga; Petra Banko; Keiichi Okimune; Taiki Azuma; Taichi Takasuka
  The Molecular Biology Society of Japan, 07 Dec. 2023, English, Poster presentation
  06 Dec. 2023 - 08 Dec. 2023
• In vitro co-expression chromatin assembly and remodeling platform for plant histone variants
  Petra Banko; Kei-ichi Okimune; Szilvia Krisztina Nagy; Taichi Takasuka
  The Molecular Biology Society of Japan, 07 Dec. 2023, English, Poster presentation
  06 Dec. 2023 - 08 Dec. 2023
• Functional histone variants-containing human chromatin assembly in vitro.
  Taiki Azuma; Taici Takasuka; Junko Haga; Kei-ichi Okimune; Petra Banko
  The Molecular Biology Society of Japan, 07 Dec. 2023, English, Poster presentation
  06 Dec. 2023 - 08 Dec. 2023
• Dissecting dual functions of a novel xylan-responsive transcriptional regulator, SsXybR, in Streptomyces sp. SirexAA-E
  Naoki Hayashi; keisuke Ohashi; Tatsuya Nagano; Vijay Kumar; Taichi takasuka
  The Molecular Biology Society of Japan, 07 Dec. 2023, English, Poster presentation
  06 Dec. 2023 - 08 Dec. 2023, 29716061;42097727
• The induced glyoxylate shunt of cellulolytic Streptomyces. sp. SirexAA-E to xylan
  Tatsuya Nagano; Keisuke Ohashi; Naoki Hayashi; Vijay Kumar; Taichi Takasuka
  The Molecular Biology Society of Japan, 07 Dec. 2023, English, Poster presentation
  06 Dec. 2023 - 08 Dec. 2023, 42097727;29716061
• Comprehensive functional characterization of aminotransferases for the construction of nitrogen metabolic pathway in E. coli.
  Shogo Hatay; Sora Fukui; Taichi Takasuka
  The Molecular Biology Society of Japan, 06 Dec. 2023, English, Poster presentation
  06 Dec. 2023 - 08 Dec. 2023
• Investigation of the Interaction Between LDL and LDL-Related Receptors Using Bio-Layer Interferometry and Atomic Force Microscopy
  武田 晴治; 三上 宏騎; 佐藤 希; 後潟 夏菜子; 高須賀 太一
  The 90th ECSJ Annual Meeting, 27 Mar. 2023, Japanese, Oral presentation
  27 Mar. 2023 - 29 Mar. 2023
• Absolute quantitative mesurement of aminotransferases in E. coli for the construction of nitrogen metabolic pathway
  Fukui Sora; Hataya Shogo; Takasuka Taichi
  The 45th Annual Meeting of the Molecular Biology Society of Japan, 02 Dec. 2022, English, Poster presentation
  30 Nov. 2022 - 02 Dec. 2022
• In vitro chromatin assembly platform for Arabidopsis thaliana
  Banko Petra; Okimune Keiichi; Nagy Szilvia; Takasuka Taichi
  The 45th Annual Meeting of the Molecular Biology Society of Japan, 01 Dec. 2022, English, Poster presentation
  30 Nov. 2022 - 02 Dec. 2022
• Fine-tuning of xylan specific gene regulations by a newly determined transcriptional regulator in Streptomyces sp. SirexAA-E.
  Hayashi Naoki; Nagano Tatsuya; Ohashi Keisuke; Takasuka Taichi
  The 45th Annual Meeting of the Molecular Biology Society of Japan, 30 Nov. 2022, English, Poster presentation
  30 Nov. 2022 - 02 Dec. 2022, 29716061
• The activation of glyoxylate shunt of cellulolytic Streptomyces. sp. SirexAA-E to Xylan.
  Nagano Tatsuya; Ohashi Keisuke; Hayashi Naoki; Kumar Vijay; Takasuka Taichi
  The 45th Annual Meeting of the Molecular Biology Society of Japan, 30 Nov. 2022, English, Poster presentation
  30 Nov. 2022 - 02 Dec. 2022, 29716061
• Development of epigenetic enzyme screening using wheat-germ co-expression nucleosome assembly method
  Okimune Keiichi; Banko Petra; Takasuka Taichi
  The 45th Annual Meeting of the Molecular Biology Society of Japan, 01 Dec. 2022, English, Poster presentation
  29 Nov. 2022 - 02 Dec. 2022
• Development of a high throughput aminotransferase assay platform using cell-free protein synthesis and nanostructure-initiator mass spectrometry (NIMS)
  Hataya Shogo; Takasuka Taichi
  Annual meeting of the molecular biology society of Japan, 03 Dec. 2021, English, Poster presentation
  01 Dec. 2021 - 04 Dec. 2021
• Development of in vitro yeast heterochromatin reconstitution using wheat germ cell-free synthesis
  Shimazu Takumi; Okimune Keiichi; Hataya Shogo; Takasuka Taichi
  Annual meeting of the molecular biology society of Japan, 03 Dec. 2021, English
  01 Dec. 2021 - 04 Dec. 2021
• Molecular mechanisms of multiple xylan-responsive transcriptional regulators in the cellulolytic Streptomyces sp. SirexAA-E.
  Ohashi Keisuke; Nakata Akane; Nagano Tatsuya; Takasuka Taichi
  Annual meeting of the molecular biology society of Japan, 03 Dec. 2021, English
  01 Dec. 2021 - 04 Dec. 2021
• Xylan-specific response of the cellulolytic Streptomyces. sp. SirexAA-E by quantitative proteomics approach
  Nagano Tatsuya; ohashi Keisuke; Nakata Akane; Takasuka Taichi
  Annual meeting of the molecular biology society of Japan, 02 Dec. 2021, English
  01 Dec. 2021 - 04 Dec. 2021
• In vitro chromatin assembly platform for Arabidopsis thaliana
  Petra Banko; Okimune Keiichi; Nagy Szilvia; Hamasaki Akinori; Morishita Ryo; Takasuka Taichi
  Annual meeting of the molecular biology society of Japan, 02 Dec. 2021, English, Poster presentation
  01 Dec. 2021 - 04 Dec. 2021
• Tetrasome and nucleosome assembly using wheat germ cell-free protein synthesis system.
  Okimune Keiichi; Hataya Shogo; Matsumoto Kazuki; Ushirogata Kanako; Banko Petra; Takeda Seiji; Takasuka Taichi
  Annual meeting of the molecular biology society of Japan, 01 Dec. 2021, English, Poster presentation
  01 Dec. 2021 - 04 Dec. 2021
• Establishment of CRISPR/Cas system in the cellulolytic Streptomyces mutants for constitutive biomass-degrading enzyme secretion
  Akane Nakata; Keisuke Ohashi; Taichi Takasuka
  The 2021 Annual Meeting of The Japan Society for Bioscience, Biotechnology and Agrochemistry, 19 Mar. 2021, Oral presentation
  18 Mar. 2021 - 21 Mar. 2021
• Mannose and mannobiose specific responses of insect associated cellulolytic Streptomyces.
  Keisuke OHASHI; Shogo HATAYA; Akane NAKATA; Kazuki MATSUMOTO; Natsumi KATO; Currie Cameron R; Fox Brian G; Taichi TAKASUKA
  The 2021 Annual Meeting of The Japan Society for Bioscience, Biotechnology and Agrochemistry, 19 Mar. 2021, Oral presentation
  18 Mar. 2021 - 21 Mar. 2021
• In vitro Drosophila nucleosome assembly by cell-free protein co-expression method
  Keiichi Okimune; Shogo Hataya; Kazuki Matsumoto; Kanako Ushirogata; Taichi Takasuka
  The 2021 Annual Meeting of The Japan Society for Bioscience, Biotechnology and Agrochemistry, 19 Mar. 2021, Oral presentation
  18 Mar. 2021 - 21 Mar. 2021
• Reconstitution of Drosophila and human chromatin by wheat germ cell-free co-expression system
  Keiichi Okimune; Szilvia Nagy; Shogo Hataya; Yaeta Endo; Taichi Takasuka
  The 43rd Annual Meeting of the Molecular Biology Society of Japan, 02 Dec. 2020, English, Poster presentation
  02 Dec. 2020 - 04 Dec. 2020
• Development of epigenetic enzyme screening method using in vitro reconsituted Drosophila chromatin
  Keiichi Okimune; Kazuki Matsumoto; Taichi Takasuka
  The 20th Annual Meeting of the Protein Science Society of Japan, Jun. 2020, English
• Development of epigenetic enzyme screening using in vitro reconstituted human chromatin
  Keiichi Okimune; Szilvia Nagy; Taichi Takasuka
  The 42nd Annual Meeting of the Molecular Biology Society of Japan, 07 Dec. 2019, English, Poster presentation
  03 Dec. 2019 - 07 Dec. 2019
• Molecular mechanisms of a novel xylan-responsive transcriptional regulator in the cellulolytic Streptomyces sp. SirexAA-E
  Akane Nakata; Keisuke Ohashi; Taichi Takasuka
  The 42nd Annual Meeting of the Molecular Biology Society of Japan, 06 Dec. 2019, English, Poster presentation
  03 Dec. 2019 - 07 Dec. 2019
• Development of epigenetic enzyme screening method using in vitro reconsituted Drosophila nucleosome.
  Kazuki Matsumoto; Szilvia Nagy; Taichi Takasuka
  The 42nd Annual Meeting of the Molecular Biology Society of Japan, 05 Dec. 2019, English, Poster presentation
  03 Dec. 2019 - 07 Dec. 2019
• Monosaccharide specific enzyme secretion in two insect symbiont Streptomyces
  Keisuke Ohashi; Kazuki Matsumoto; Taichi Takasuka
  日本農芸化学会2018年度大会, 19 Mar. 2018, English
• In vitro reconstitution of PCNA-p15 centered DNA replication and repair complexes
  Kazuki Matsumoto; Taichi Takasuka
  Consortium of Biological Sciences 2017, 08 Dec. 2017, English
  06 Dec. 2017 - 09 Dec. 2017
• Biochemical and proteomics characterization of the plant biomass-degrading potential of insect associated Streptomyces spp.
  Keisuke Ohashi; Taichi Takasuka
  Consortium of Biological Sciences 2017, 07 Dec. 2017, English
  06 Dec. 2017 - 09 Dec. 2017
• Phylogenetic-guided PDI functional annotation to establish in vitro oxidative protein folding
  Nagy Szilvia; Taichi Takasuka
  Consortium of Biological Sciences 2017, 06 Dec. 2017, English
  06 Dec. 2017 - 09 Dec. 2017
• Responses of cellulolytic Streptomyces to plant biomass
  Taichi Takasuka
  Consortium of Biological Sciences 2017, 06 Dec. 2017, English
  06 Dec. 2017 - 09 Dec. 2017
• Workshop "Diversity of academic career"
  Taichi Takasuka
  Consortium of Biological Sciences 2017, Japanese, Nominated symposium
  06 Dec. 2017 - 09 Dec. 2017
• Omics and genome-enabled technology to understand phylogenetic-based enzyme functions
  Taichi Takasuka
  環境微生物系学会合同大会2017, 31 Aug. 2017, English, Invited oral presentation
  29 Aug. 2017 - 31 Aug. 2017, [Invited]
• Phylogenetic-guided biochemical annotation for glycoside hydrolase families
  Taichi Takasuka
  The 15th Annual Meeting of the Protein Science Society of Japan, 26 Jun. 2015, English, Invited oral presentation
  24 Jun. 2015 - 26 Jun. 2015, [Invited]
• Establishment of a method for extracting useful monosaccharides and natural fucoidan from algal biomass using multiple brown algae degrading enzymes
  Sora Fukui; Taichi Takasuka; Masashi Hosokawa; Hideyuki Kurihara; Shota Atsumi
  The Molecular Biology Society of Japan, 06 Dec. 2012, English, Poster presentation
  06 Dec. 2012 - 08 Dec. 2012, 29716010;29716088

• ワンダーフォーゲル実習Ⅵ, 2024年, 博士後期課程, 国際食資源学院
• 大学院共通授業科目（一般科目）：自然科学・応用科学, 2024年, 修士課程, 大学院共通科目
• バイオテクノロジー学特論, 2024年, 修士課程, 農学院
• バイオテクノロジー学特論演習, 2024年, 修士課程, 農学院
• 食資源生産論, 2024年, 修士課程, 国際食資源学院
• 大学院共通授業科目（一般科目）：自然科学・応用科学, 2024年, 修士課程, 大学院共通科目
• 食資源特別講義, 2024年, 修士課程, 国際食資源学院
• 分子細胞生物学, 2024年, 学士課程, 農学部
• 応用生命科学実験, 2024年, 学士課程, 農学部
• 応用生命科学概論, 2024年, 学士課程, 農学部
• 分子生物学, 2024年, 学士課程, 農学部

• THE JAPANESE BIOCHEMICAL SOCIETY
• The American Chemical Society
• THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

• Expansion of microbial co-culture–based production technologies for value-added products utilizing plant biomass
  ヤンマー資源循環支援機構研究助成
  Apr. 2026 - Mar. 2028
  Taichi Takasuka
  YESSA, Principal investigator
• Elucidation of the intestinal survival strategy of bifidobacteria through the coordination of amino acid biosynthesis and acquisition
  Grants-in-Aid for Scientific Research
  Apr. 2024 - Mar. 2028
  吹谷 智; 高須賀 太一
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 24K01658
• 新規セルロース分解性バチルス菌による植物バイオマスからPHAの一気通貫生産
  科学研究費助成事業
  27 Jun. 2025 - 31 Mar. 2027
  高須賀 太一
  日本学術振興会, 挑戦的研究(萌芽), 北海道大学, 25K22375
• Consolidated biomass to bioplastic conversion using a co-cultivation system
  Grants-in-Aid for Scientific Research
  01 Apr. 2023 - 31 Mar. 2027
  高須賀 太一; FOX BRIAN
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 23K27036
• In vitro reconstitution and visualization of heterochromatin in Saccharomyces cerevisiae
  一般研究助成
  Apr. 2025 - Mar. 2027
  Takasuka Taichi
  Institute for Fermentation, Osaka (IFO), Principal investigator, G-2025-2-039
• 独自共培養系による植物バイオマスのダイレクトなバイオプラスチック転換技術の開発
  科学研究費助成事業
  Apr. 2023 - Mar. 2027
  高須賀 太一
  日本学術振興会, 基盤研究(B), 北海道大学, 23H02343
• Consolidated PHA production from marine biomass
  ALCA-Next（Advanced Technologies for Carbon-Neutral）
  Sep. 2024 - Mar. 2026
  Takasuka Taichi; Maeda Tomoya
  Japan Science and Technology Agency, JPMJAN24D6
• 海洋バイオマスからPHA一貫生産技術の確立
  2024 - 2025
  高須賀 太一
  本研究では、独自技術である共培養系による褐藻バイオマス中の多糖および単糖類をターゲットにし、褐藻バイオマス原料からPHAの一気通貫生産を実証する。本共培養系については、育種予定の生態系微生物集団を利用する事で、褐藻バイオマスの分解向上、将来的にはPHAやそれ以外の化成品等の共培養一貫生産技術開発も視野に入れている。
  科学技術振興機構, 戦略的な研究開発の推進/戦略的創造研究推進事業/ALCA-Next, 24021388
• Consolidated bioconversion of cellulosic biomass to PHAs by one cell factory
  Grants-in-Aid for Scientific Research
  08 Mar. 2023 - 31 Mar. 2024
  高須賀 太一; KUMAR VIJAY
  未利用植物性バイオマスを原料としたポリヒドロキシアルカノエート（PHA）の生産は、持続可能なPHAベースの生分解性バイオプラスチックを実現する理想的な方法と考えられる。本研究では、植物バイオマスをPHAに一貫生産することを目的とし、セルロース分解能力を持つ昆虫共生放線菌Streptomyces sp. SirexAA-EとPHAを生産する事で知られるPriestia megateriumの2つの特殊な細菌を共培養することによって、統合された生物変換を実証した。単独培養では、S. sp. SirexAA-EはPHAを生産しない事を確認し、またP. megateriumは植物ポリ糖類で生育しない事を確認した。一方、共培養を行ったところ、セルロース、キシラン、マンナンなどの精製された多糖類および植物バイオマス（ミスカンサス、トウモロコシの茎や葉）を単一炭素源とし、ポリ（3-ヒドロキシ酪酸）（PHB）の生産が確認できた。さらに、S. sp. SirexAA-EとP. megateriumの共培養によって、ミスカンサスあたり40 mgのPHBの生産を確認した。したがって、植物バイオマスを原料としたPHA一貫生産技術の実施可能性を示す事ができたと考える。本研究を実施した結果、特許の出願（特願2022-074-JP01 ）および、2023年にBioresource Technol誌への掲載を実績として報告済みである（Kumar et al., Bioresource Technol, 2023）。
  Japan Society for the Promotion of Science, Grant-in-Aid for JSPS Fellows, Hokkaido University, 22KF0007
• Enzymatic conversion of kelp biomass for biofuels and health beneficial products.
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
  Apr. 2020 - Mar. 2024
  FOX BRIAN; 高須賀 太一; 細川 雅史; 栗原 秀幸
  Our aim of the current project is to develop an efficient method to depolymerize natural kelps available in the Hokkaido ocean by a combination of several enzyme functions, including laminarin-degrading enzymes and alginate-degrading enzymes. A set of kelp spp. were obtained and ready to analytically evaluate their chemical compositions for the next fiscal year. Regarding a laminarin-degrading enzyme, we had picked up a glycoside hydrolase 55 enzyme (GH55) from a highly cellulolytic insect symbiont bacterium (Bianchetti and Takasuka et al., 2015, Journal of Biological Chemistry). 7 polysaccharide lyases 18 enzymes (PL18s), which function in alginate degradation, were selected from 7 different bacterial species, and four PL18s had been recombinantly expressed and subjected to the initial enzyme screening by using pure alginic acid substrate. Moreover, two polysaccharide lyase 17 enzymes (PL17s), were gene synthesized and cloned into the bacterial expression vector. From the previous studies, PL18 is shown to be the endo-type polysaccharide lyase, which produces oligoalginic acids (>3 degrees of polymerization (DP)), while PL17 should catalyze an exo-type reaction. In the FY2021, collected kelps will be tested to be hydrolyzed by at least three different enzymes, GH55, PL17, and PL18, and end products will be analyzed.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 20H02776
• ワンセルファクトリーによるセルロース系バイオマスのPHAsへの一貫生産技術の確立
  科学研究費助成事業 特別研究員奨励費
  13 Nov. 2020 - 31 Mar. 2023
  高須賀 太一
  日本学術振興会, 特別研究員奨励費, 北海道大学, 20F20391
• 糸状菌由来の新規多糖分解酵素の探索と機能・構造解析
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
  Apr. 2020 - Mar. 2023
  志水 元亨; 高須賀 太一
  今年度、研究代表者らはペクチンのみを炭素源として生育させた際にA. nidulansが細胞外に分泌する、機能が分かっている酵素などとアミノ酸配列レベルで全く相同性を有さず、かつシグナル配列（セルラーゼなどの細胞外酵素が有する）を持つ16種の機能未知タンパク質 (hypothetical protein; HP) の機能について解析した。それらの中で2種のHPの機能を明らかに出来た。その2種のうち、新規のペクチン分解酵素 (rhamnogalacturonan lyase) の詳細な機能と立体構造を明らかにした。
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Meijo University, 20K05815
• Determining molecular mechanisms of transcriptional regulation and genome engineering toward efficient biomass degradation.
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
  Apr. 2020 - Mar. 2023
  高須賀 太一
  当該年度は、森林食害性昆虫共生放線菌であるStreptomyces sp. SirexAA-E株について、本株の生育環境中のセルロースやヘミセルロース高分子に対する応答を司る転写制御因子の同定および遺伝子発現ネットワークの解明を目的として研究を実施した。特に木質の主要なヘミセルロース成分の一つであるマンナン糖鎖への細胞応答について、２種類の異なるLacI様転写リプレッサー因子（SsCebRとSsManR）が関与している事を見出した。SsCebRは分泌セルラーゼをコードする遺伝子上流に結合し、セルロースやセロオリゴ糖非存在下では、下流の遺伝子群を抑制する事が報告されており、セロオリゴ糖存在下において、セロオリゴ糖のSsCebRへの結合によって、SsCebRがゲノムDNAから解離する事が知られていたが、本研究では生化学的手法によって、マンノオリゴ糖の１つであるマンノビオースによってもSsCebRが、ゲノムDNAから解離する事を明らかにした。また、新規転写リプレッサーとしてSsManRが、分泌マンナン分解酵素をコードする遺伝子上流に結合する事、およびマンノースおよびマンノビオースがSsManRのゲノムDNAからの解離を誘導する事を生化学的手法によって明らかにした。これらのin vitro実験系から得た知見をin vivoで試験するため、Streptomyces sp. SirexAA-E株をマンノビオースを単一炭素源として用いた培地で培養し、分泌タンパクプロテオミクス解析したところ、SsCebRおよびSsManR結合領域下流の遺伝子にコードされた分泌酵素の生産が確認できた。本研究成果はApplied and Environmental Microbiologyにて掲載された。
  Streptomyces sp. SirexAA-E株のゲノム編集についても、現在上記のSsCebR遺伝子欠損候補株が得られている。
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 20K06139
• A molecular framework for polar localization of nutrient transport proteins in the plasma membrane of plant cells
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
  Apr. 2019 - Mar. 2023
  高野 順平; 高須賀 太一
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Osaka Prefecture University, 19H00934
• Development of consolidated bioprocessing of macroalgae biomass to produce bioproducts
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
  Apr. 2018 - Mar. 2023
  渥美 正太; 高須賀 太一; 堀 千明
  効率的な糖化に向けた褐藻分解酵素（糖質分解酵素）の分泌と分解産物の化成品への転換を行える大腸菌の作出を行っており、これまで褐藻の主要成分であるラミナリン、アルギン酸、ならびにマニトール単糖に着目し、研究を行った。
  遺伝子組換え対象微生物である大腸菌は、マニトールを単一炭素源として生育する事が確認できた。また、ラミナリン分解酵素として糖質分解酵素ファミリーGH55の褐藻中のラミナリン分解能力の解明とアルギン酸分解のための糖質リアーゼファミリーPL18の褐藻中のアルギン酸分解における機能解析を行っている。GH55については、褐藻中の長鎖ラミナリンをほぼ全て可溶性のオリゴ糖や単糖分解する事を確認した。アルギン酸長鎖については、マヌロン酸で構成されるM-blockとグルロン酸で構成されるG-block、及びそれら2種の糖が混合したM/G-blockから構成されるが、いずれの構成糖成分においても、PL18が加水分解する事を確認した。
  これらの酵素を用いた後の、褐藻成分分解物の利用については代謝デザインを行っており、イソブタノール以外の有用化成品についても検討中である。
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 18H02011
• Genemic assessment of symptom on beech forest decline
  Grants-in-Aid for Scientific Research
  01 Apr. 2017 - 31 Mar. 2021
  Saito Hideyuki
  An early detection of physiological symptom before getting weaker of tree vigor is important to prevent forest decline. As part of a development research of environmental assessment technique of forest health using genomics, here we elaborate Fagus crenata draft genome and conducted the genome-wide survey of the symptom indicator by transcriptome and proteome analyses for F. crenata leaves. The investigation of natural beech forests across Japan resulted a selection of candidate gene, chloroplast ribosomal RNA. The quantitative change of ribosome generally affects the capacity of translational regulation in gene expression, probably affecting the physiological response to the environmental changes. We concluded the ribosomal RNA gene is a promising gene for assessing the symptom of forest decline.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 17H01884
• Secretome analysis of insect-symbiont highly cellulolytic bacteria for biomass utilization
  Research grant
  Apr. 2018 - Mar. 2019
  Takasuka Taichi
  Inamori Foundation, Principal investigator, Competitive research funding
• Ecological function of wood insect associated microbes(Fostering Joint International Research)
  Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research)
  2016 - 2018
  Takasuka Taichi; Fox Brian; Yoshikuni Yasuo
  The establishment of a sustainable biofuels and biochemicals production technology using biomass resources in land and sea areas is required as an alternative resource for finite fossil resources that are expected to be diminished in the future. So that we aimed at the development of efficient decomposition method of biomass crops in land and kelp in the sea area.
  Efficient plant biomass decomposition requires novel biomass-degrading enzymes, which might possess multi-substrate functions and high specific activities. To discover the new enzyme function and also to understand multifunctionality of the cellulase, we focused on 243 enzymes from the previously reported multifunctional enzyme family (GH5_4s) for functional characterization together with the Bayesian inference phylogenetic analyses.
  Additionally, an efficient kelp degradation was achieved by a minimum two enzyme combination between Glycoside hydrolase 55 family (GH55) and alginate lyase (PL18).
  Japan Society for the Promotion of Science, Fund for the Promotion of Joint International Research (Fostering Joint International Research), Hokkaido University, 15KK0269
• Ecological function of wood insect associated microbes
  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
  Apr. 2015 - Mar. 2017
  Takasuka Taichi
  In this study, we attempted to identify the woodwasp symbiont microbes from Hokkaido forest. We captured two wood wasp species, Sirex nitobei and Xiphydria cannelus. One bacteria and one fungus were isolated from a mygcangia of X. canelus, and were identified as Rahnella spp., and Daldinia decipiens species by 16s rRNA and ITS sequencing, respectively. Furthermore, we determined the hemicellulose-degrading activity in the supernatant when D. decipiens was cultured in the liquid medium containing polysaccharides such as cellulose, hemicellulose, wood biomass as sole carbon source, and glucose was used as a control culture. By utilizing proteomics, we were able to determined multiple glycoside hydrolase family enzymes, which are predicted to break down various polysaccharides present in woody biomass.
  Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, 15K18812

• Application of kelp-degrading bacteria.
  Design right, Nagano Tatsuya, Mitsumasu Saki, Maeda Tomoya, Takasuka Taichi
  特願2025-123157, Aug. 2025
• In vitro reconstitution of H3-H4 octasomes
  Patent right, Banko P.; Okimune K.; Azuma T.; Haga J.; Takasuka T.
  特願2023-188455, 2023
• In vitro plant chromatin assembly method.
  Patent right, Banko Petra; Okimune Keiichi; Takasuka Taichi
  特願2022-071114, 22 Apr. 2022
• Consolidated polyhydroxyalkanoate production.
  Design right, Kumar V; Takasuka T
  特願2023-009430, 2022
• Nucleosome assembly method using a cell-free protein synthesis platform
  Patent right, Endo Yaeta; Takasuka Taichi
  特願WO2019/102516A1, 21 Nov. 2017
• Method and Compositions for Improved Lignocellulosic Material Hydrolysis.
  Patent right, Taichi E. Takasuka; Brian G. Fox; Adam J. Book; Cameron R. Currie
  特願 20130189744
• Multifunctional Cellulase And Hemicellulase.
  Patent right, Taichi E. Takasuka; Brian G. Fox; Christopher M. Bianchetti
  特願US20140079683 A1