Researcher basic information

• Ph.D, Institute of Science Tokyo

• https://researchmap.jp/7000014923?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201601017616557142

• 201601017616557142

• 骨格系の進化医学
• 口腔顎顔面の進化医学
• Neuro-Skeletal Pharmacology
• Metabolic Bone Disease
• Skeletal Development
• Bone Metabolism

• Life Science, Oral biological science, Body Plan and Skeletal Development
• Life Science, Oral biological science, Biochemistry and Molecular Biology・Pharmacological Sciences

• Bachelor's degree program, School of Dental Medicine
• Doctoral (PhD) degree program, Graduate school of Dental Medicine

Research activity information

• Jul. 2018, Japanese Society for Bone and Mineral Research, Academic Award
  IIMURA Tadahiro
• Sep. 2010, Japanese Association for Oral Biology, Academic Award
  IIMURA Tadahiro
• Apr. 2010, 日本歯科骨粗鬆症研究会, 優秀演題賞
  飯村 忠浩
• May 2008, Japanese Society for Developmental Biologists, Most Down-Loaded Paper Award
  IIMURA Tadahiro
• Mar. 1999, International Association for Dental Research, Hatton Travel Award
  IIMURA Tadahiro

• A Case of Mandibular Osteomyelitis Occurring in a Patient With Parry-Romberg Syndrome
  Riyu Koguchi-Yoshida; Michita Tayama; Hiroshi Kaneko; Haruhisa Watanabe; Tadahiro Iimura; Yutaka Maruoka
  Cureus, Springer Science and Business Media LLC, 12 Dec. 2025
  Scientific journal
• Bone Metabolic Changes and Osteoporosis During Pregnancy and Lactation: A View from Dental Medicine.
  Mai Nishiura; Haruhisa Watanabe; Atsuko Nakanishi-Kimura; Marie Hoshi-Numahata; Shinnosuke Nishimoto; Fumi Ueno; Riyu Koguchi; Ryutaro Takemoto; Yusuke Kurakane; Lang Bao; Tadahiro Iimura
  International journal of molecular sciences, 26, 21, 28 Oct. 2025, [International Magazine]
  English, Scientific journal, Pregnancy- and lactation-associated osteoporosis (PLO) is receiving increasing attention. During pregnancy and lactation, bone metabolism is dramatically changed to supply minerals to the fetus and infant, which is a major cause of PLO. Weaning of lactation is clinically a primary choice to treat lactation-induced osteoporosis since breastfeeding is a key regulator of the pathophysiology during lactation. However, breastfeeding is beneficial to the physical and mental development of infants. We also discuss the beneficial effects of breastfeeding on the oral and maxillofacial development of infants. Pharmacological treatment of PLO is also discussed. This review also discusses how dynamic regulatory changes in bone metabolism during pregnancy and lactation affect homeostasis of the temporomandibular joint (TMJ) and alveolar bone in mothers, from the perspectives of TMJ diseases and orthodontic treatment.
• Establishment of artificial intelligence-driven fluorescence morphometry reveals involvement of osteocyte perilacunar remodeling specifically in mandibular bone of ovariectomized rats.
  Atsuko Nakanishi-Kimura; Haruhisa Watanabe; Marie Hoshi-Numahata; Masae Goseki-Sone; Tadahiro Iimura
  Microscopy (Oxford, England), 17 Oct. 2025, [International Magazine]
  English, Scientific journal, Bone dynamically changes its shape and structure in response to extra-tissue environments, so that bone morphometry has been a substantial method to evaluate pathophysiology of bone. Osteocytes embedded in mineralized bone matrix play key roles in systemic bone metabolism and characterize distinct bone sites. The jawbone has been described as a unique bone in the context of vertebrate evolution and function. Bone loss in the mandibular bone is less obvious in osteoporotic conditions than in other bones, such as vertebral and limb long bones, both in animal models and in clinical studies. Since osteocyte lacunae are complex and small (-10µm in length) in shape and size, respectively, comprehensive and unbiased morphometrical analysis of changes in the size of osteocyte lacunae was still an obstacle. This study established an artificial intelligence-driven morphometry with wide-field microscopy-based imaging of osteocyte lacunae. Successive comparative analyses demonstrated active perilacunar bone remodeling in the mandibular bone than in the parietal bone. This approach enabled us to statistically compare morphometric parameters in a more comprehensive and unbiased manner. We further discuss the possible unique contribution of the mandibular bone to the pathophysiology of osteoporosis. This study established an artificial intelligence-driven morphometry with wide-field microscopy-based imaging of osteocyte lacunae. Successive comparative analyses demonstrated active perilacunar bone remodeling in the mandibular bone than in the parietal bone. This approach enabled us to statistically compare morphometric parameters in a more comprehensive and unbiased manner.
• 細胞外基質ラミニンは褐色細胞腫由来PC-12細胞の脱分化を抑制し,本来の副腎髄質細胞機能を回復する
  西浦 まい; 渡辺 陽久; 飯村 忠浩
  日本口腔科学会雑誌, 74, 2, 118, 118, (NPO)日本口腔科学会, Jul. 2025
  Japanese
• 細胞外基質ラミニンは褐色細胞腫由来PC-12細胞の脱分化を抑制し,本来の副腎髄質細胞機能を回復する
  西浦 まい; 渡辺 陽久; 飯村 忠浩
  日本口腔科学会雑誌, 74, 2, 118, 118, (NPO)日本口腔科学会, Jul. 2025
  Japanese
• SARS-CoV-2 infection promotes lung thrombosis by inducing integrinβ3 expression in vascular endothelial cells.
  Wataru Ito; Yuya Sakurai; Nako Maishi; Ryo Takeda; Takahito Teshirogi; Li Yu; Yasuhiro Hida; Michihito Sasaki; Yasuko Orba; Takuya Tsumita; Haruhisa Watanabe; Tadahiro Iimura; Terufumi Kubo; Shinsuke Toba; Akihiko Sato; Aya Matsuda; Daisuke Kyuno; Makoto Osanai; Yoichi Ohiro; Toshihiko Torigoe; Hirofumi Sawa; Kyoko Hida
  Scientific reports, 15, 1, 20447, 20447, 01 Jul. 2025, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Severe COVID-19 shows a high incidence of pulmonary thrombosis. However, the molecular mechanism underlying this phenomenon remains unclear. We have performed RNA sequencing of isolated endothelial cells (ECs) from infected mid-aged and young mice. Compared to young mice, Integrinβ3 (ITGB3) expression levels were higher in ECs of mid-aged mice which showed thrombosis in lungs. SARS-CoV-2 exposure increased the number of adhered platelets on the EC monolayer in vitro. Knockdown of ITGB3 in ECs decreased platelet adhesion to them. Among the molecules known as SARS-CoV-2 receptors, Kringle-containing transmembrane protein 1 contributed to ITGB3 upregulation in ECs by SARS-CoV-2. Histological analysis showed that ITGB3-positive blood vessels were frequently detected not only in infected-mid-aged mouse lungs but also in COVID-19-affected human autopsy lungs. This study suggests that the induction of ITGB3 expression in ECs is one of the mechanisms of thrombosis in severe COVID-19 pneumonia.
• A Long-Term (41 Years) Radiographic Follow-Up Study of the Onset and Development of a Jawbone Lesion in a Patient With Gnathodiaphyseal Dysplasia.
  Riyu Koguchi; Haruhisa Watanabe; Mai Nishiura; Tadahiro Iimura; Yutaka Maruoka
  Cureus, 17, 7, e88763, Jul. 2025, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Gnathodiaphyseal dysplasia (GDD) is an autosomal dominant syndrome characterized by bone fragility, sclerosis of tubular bones, and cemento-osseous lesions of the jawbones. This report describes the long-term radiographic follow-up study of jaw lesions in a GDD-affected woman of >40 years of age from the age of one onward. At three years of age, widening of the diaphyseal cortices in the femur and radius was observed, although no symptoms were observed in the jawbones. At nine years of age, we observed a slightly sclerotic appearance in the alveolar and jawbones adjacent to the permanent tooth roots. After completion of the secondary dentition at 14 years of age, increased density of the sclerotic mass was clearly observed. Although the etiology and pathogenesis are uncertain, this study observed that the onset of the jawbone lesion had already appeared after the mixed dentition stage, and the sclerotic mass developed with age.
• Chemokine receptor 5 signaling in oral diseases and degenerative temporomandibular joint disease.
  Haruhisa Watanabe; Riyu Koguchi; Takashi S Kajii; Yutaka Maruoka; Tadahiro Iimura
  Journal of oral biosciences, 67, 2, 100666, 100666, Jun. 2025, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, BACKGROUND: Chemokine receptor 5 (CCR5)-mediated signals are involved in various biological responses and inflammatory diseases. Recent studies have revealed the roles of this signaling pathway in bone metabolism, metabolic bone diseases, and joint diseases. HIGHLIGHT: Through preclinical and clinical studies, our research group has demonstrated that CCR5 signaling is deeply involved in degenerative changes in the temporomandibular joint (TMJ). CONCLUSION: In this short review, we outline the diverse functions of CCR5 signaling in oral and degenerative TMJ diseases.
• Genome-wide analyses of susceptibility genes responsible for mandibular prognathism in the Japanese population.
  Marie Hoshi-Numahata; Atsuko Nakanishi-Kimura; Haruhisa Watanabe; Mai Nishiura; Shinnosuke Nishimoto; Fumi Ueno; Riyu Koguchi; Akira Oka; Yoshiaki Sato; Takashi S Kajii; Tadahiro Iimura
  Journal of oral biosciences, 67, 2, 100670, 100670, Jun. 2025, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, BACKGROUND: Mandibular prognathism (MP) is a type of malocclusion characterized by an imbalance in the anteroposterior position of the upper and lower jaws. The prevalence of MP in Japan is relatively high, suggesting a unique genetic background in the population. HIGHLIGHT: Genome-wide analyses identified susceptibility genes responsible for mandibular prognathism in the Japanese population. CONCLUSION: Identification of the genes associated with malocclusion will pave the way for personalized and precise medicine and contribute to craniofacial biology.
• Cholesterol metabolism and neuroinflammatory changes in a non-human primate spinal nerve ligation model.
  Hiroshi Yamane; Suguru Koyama; Takayuki Komatsu; Tomoya Tanaka; Riyu Koguchi; Haruhisa Watanabe; Mai Nishiura; Satoru Yoshikawa; Tadahiro Iimura
  Scientific reports, 15, 1, 11462, 11462, 03 Apr. 2025, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Neuropathic pain remains one of the major neurological conditions with high unmet medical needs. Poor translation from preclinical studies using rodent models to clinical trials is one of the major obstacles to the development of new pharmacological medications to treat neuropathic pain. The aims of this study were to establish a behavioral test to evaluate spontaneous pain in a spinal nerve ligation (SNL) model using cynomolgus monkey as a non-human primate (NHP) model. After right unilateral L7 SNL surgery in cynomolgus monkeys, the percentage of weight-bearing on ipsilateral hindlimb significantly decreased, which was well-associated with an analytical score of electroencephalography (EEG). Transcriptomic analysis of RNA-seq results from the dorsal part of the spinal cord identified pathways matching those in equivalent rodent models, along with NHP-specific pathways, suggesting that neuroinflammation and cholesterol transportation/metabolism were the main pathways altered in this NHP model. Additionally, several upregulated genes observed here were previously reported uniquely in clinical studies, but not in rodent models. This study provides a potentially useful model that can aid our understanding of pathophysiological mechanism of neuropathic pain and the development of pain relief therapies by inducing a robust behavioral phenotype and changes in gene expression resembling those in patients.
• Selective Pyk2 inhibition enhances bone restoration through SCARA5-mediated bone marrow remodeling in ovariectomized mice.
  Yunqing Liu; Mai Nishiura; Mika Fujii; Sumiti Sandhu; Yasutaka Yawaka; Yutaka Yamazaki; Akira Hasebe; Tadahiro Iimura; Sek Won Kong; Ji-Won Lee
  Cell communication and signaling : CCS, 22, 1, 561, 561, 22 Nov. 2024, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Understanding the intricate cellular interactions involved in bone restoration is crucial for developing effective strategies to promote bone healing and mitigate conditions such as osteoporosis and fractures. Here, we provide compelling evidence supporting the anabolic effects of a pharmacological Pyk2 inhibitor (Pyk2-Inh) in promoting bone restoration. In vitro, Pyk2 signaling inhibition markedly enhances alkaline phosphatase (ALP) activity, a hallmark of osteoblast differentiation, through activation of canonical Wnt/β-catenin signaling. Notably, analysis of human mesenchymal stem cells through RNA-seq revealed a novel candidate, SCARA5, identified through Pyk2-Inh treatment. We demonstrate that Scara5 plays a crucial role in suppressing the differentiation from stromal cells into adipocytes, and accelerates lineage commitment to osteoblasts, establishing Scara5 as a negative regulator of bone formation. Additionally, Pyk2 inhibition significantly impedes osteoclast differentiation and bone resorption. In a co-culture system comprising osteoblasts and osteoclasts, Pyk2-Inh effectively suppressed osteoclast differentiation, accompanied by a substantial increase in the transcriptional expression of Tnfrsf11b and Csf1 in osteoblasts, highlighting a dual regulatory role in osteoblast-osteoclast crosstalk. In an ovariectomized mouse model of osteoporosis, oral administration of Pyk2-Inh significantly increased bone mass by simultaneously reducing bone resorption, promoting bone formation and decreasing bone marrow fat. These results suggest Pyk2 as a potential therapeutic target for both adipogenesis and osteogenesis in bone marrow. Our findings underscore the importance of Pyk2 signaling inhibition as a key regulator of bone remodeling, offering promising prospects for the development of novel osteoporosis therapies.
• 骨形成促進薬PTH製剤は骨粗鬆症モデルラットの顎骨特異的に骨細胞性骨リモデリング調節を行う AI駆動型形態計測による解析
  中西 徳子[木村]; 星 麻里絵[沼端]; 渡辺 陽久; 西浦 まい; 飯村 忠浩
  Journal of Oral Biosciences Supplement, 2024, [P1, 33], (一社)歯科基礎医学会, Nov. 2024
  Japanese
• 顎関節退行性病変における臨床病態解明とケモカインCCL5の関与
  渡辺 陽久; 西浦 まい; 星 麻里絵[沼端]; 中西 徳子[木村]; 飯村 忠浩
  Journal of Oral Biosciences Supplement, 2024, [P2, 40], (一社)歯科基礎医学会, Nov. 2024
  Japanese
• 褐色細胞腫の分化誘導療法を基盤とした新規薬物療法の探索
  西浦 まい; 渡辺 陽久; 中西 徳子[木村]; 星 麻里絵[沼端]; 八若 保孝; 飯村 忠浩
  Journal of Oral Biosciences Supplement, 2024, [P2, 41], (一社)歯科基礎医学会, Nov. 2024
  Japanese
• AI駆動型形態計測を用いた頭蓋骨・下顎骨における骨形成促進薬テリパラチドの薬理作用特異性
  中西 徳子[木村]; 高倉 綾; 星 麻里絵[沼端]; 佐藤 嘉晃; 高尾 亮子; 飯村 忠浩
  日本骨形態計測学会雑誌, 34, 2, 80, 81, 日本骨形態計測学会, Nov. 2024
  Japanese
• 骨形成促進薬PTH製剤は骨粗鬆症モデルラットの顎骨特異的に骨細胞性骨リモデリング調節を行う AI駆動型形態計測による解析
  中西 徳子[木村]; 星 麻里絵[沼端]; 渡辺 陽久; 西浦 まい; 飯村 忠浩
  Journal of Oral Biosciences Supplement, 2024, [P1, 33], (一社)歯科基礎医学会, Nov. 2024, [Last author, Corresponding author]
  Japanese
• 顎関節退行性病変における臨床病態解明とケモカインCCL5の関与
  渡辺 陽久; 西浦 まい; 星 麻里絵[沼端]; 中西 徳子[木村]; 飯村 忠浩
  Journal of Oral Biosciences Supplement, 2024, [P2, 40], (一社)歯科基礎医学会, Nov. 2024, [Last author, Corresponding author]
  Japanese
• 褐色細胞腫の分化誘導療法を基盤とした新規薬物療法の探索
  西浦 まい; 渡辺 陽久; 中西 徳子[木村]; 星 麻里絵[沼端]; 八若 保孝; 飯村 忠浩
  Journal of Oral Biosciences Supplement, 2024, [P2, 41], (一社)歯科基礎医学会, Nov. 2024, [Last author, Corresponding author]
  Japanese
• AIとGISによる新規形態計測法の確立とイヌ皮質骨リモデリングに対するテリパラチドの薬理作用解明
  星 麻里絵[沼端]; 高倉 綾; 中西 徳子[木村]; 渡辺 陽久; 高田 健太郎; 西浦 まい; 佐藤 嘉晃; 高尾 亮子[川端]; 飯村 忠浩
  日本骨形態計測学会雑誌, 34, 2, 76, 77, 日本骨形態計測学会, Nov. 2024, [Invited], [Last author, Corresponding author]
  Japanese
• 女性における顎関節の退行性病変の病態と血清CCL5値の上昇との関連性
  渡辺 陽久; 中西 徳子[木村]; 星 麻里絵[沼端]; 丸岡 豊; 北川 善政; 飯村 忠浩
  日本骨形態計測学会雑誌, 34, 2, 78, 79, 日本骨形態計測学会, Nov. 2024, [Invited], [Last author, Corresponding author]
  Japanese
• 骨粗鬆症モデルラットへの骨形成促進薬PTH製剤投与による下顎骨の骨小腔周囲性骨リモデリング調節
  中西 徳子; 星 麻里絵; 佐藤 嘉晃; 飯村 忠浩
  日本矯正歯科学会大会プログラム・抄録集, 83回, 186, 186, (公社)日本矯正歯科学会, Oct. 2024
  Japanese
• ゲノムワイド解析による下顎前突症疾患感受性遺伝子の探索と同定
  星 麻里絵[沼端]; 梶井 貴史; 中西 徳子[木村]; 渡辺 陽久; 西浦 まい; 上野 楓実; 西本 慎之介; 孝口 里侑; 佐藤 嘉晃; 飯村 忠浩
  北海道歯学雑誌, 45, 12, 15, 北海道歯学会, Sep. 2024, [Last author, Corresponding author]
  Japanese, 下顎前突症には遺伝学的要因が深く関わることが古くから知られており、現在では下顎前突症の大半は多因子遺伝に基づく表現型であると考えられている。今回、下顎前突症の原因となる疾患感受性遺伝子の同定に関するこれまでの知見を以下の項目に分けて概説した。1)下顎前突症におけるゲノムワイド連鎖解析。2)ゲノムワイド連鎖解析の代替戦略とされたゲノムワイド関連研究。3)下顎前突症における疾患感受性候補遺伝子(PLXNA2とBEST3)の同定。
• 副甲状腺ホルモン1型受容体作動薬の顎骨への薬理作用と歯科矯正治療への応用
  中西 徳子[木村]; 星 麻里絵[沼端]; 上野 楓実; 西本 慎之介; 西浦 まい; 孝口 里侑; 渡辺 陽久; 佐藤 嘉晃; 飯村 忠浩
  北海道歯学雑誌, 45, 16, 21, 北海道歯学会, Sep. 2024, [Last author, Corresponding author]
  Japanese, 骨粗鬆症治療薬は骨吸収抑制薬と骨形成促進薬に大別され、前者の代表的な薬剤にはビスホスホネート製剤やRANKL抗体製剤、後者にはPTH1型受容体作動薬(PTH1R作動薬)や抗スクレロスチン抗体製剤がある。骨粗鬆症治療薬の薬理作用はこれまで主に体幹・四肢骨で評価されており、顎骨への作用については不明な点が多い。今回、PTHに関連した顎骨疾患について解説するとともに、PTH1R作動薬投与による顎骨への影響、PTH1R作動薬投与の歯科矯正治療への応用についてこれまでの知見を概説した。
• 抗老化作用に注目した骨再生応用のためのヒト骨髄由来間葉系幹細胞培養条件の探索
  渡辺 陽久; 北川 善政; 飯村 忠浩
  日本口腔科学会雑誌, 73, 2, 174, 175, (NPO)日本口腔科学会, Sep. 2024, [Last author, Corresponding author]
  Japanese
• Enhanced phagocytosis associated with multinucleated microglia via Pyk2 inhibition in an acute β-amyloid infusion model
  Ji-Won Lee; Kaito Mizuno; Haruhisa Watanabe; In-Hee Lee; Takuya Tsumita; Kyoko Hida; Yasutaka Yawaka; Yoshimasa Kitagawa; Akira Hasebe; Tadahiro Iimura; Sek Won Kong
  Journal of Neuroinflammation, 21, 1, Springer Science and Business Media LLC, 06 Aug. 2024, [Peer-reviewed]
  Scientific journal
• 骨粗鬆症モデルラットへのテリパラチド投与による下顎骨骨小腔の3次元形態変化 骨細胞性・骨小腔周囲リモデリング調節の可能性
  中西 徳子[木村]; 高倉 綾; 星 麻里絵[沼端]; 高尾 亮子; 飯村 忠浩
  日本骨代謝学会学術集会プログラム抄録集, 42回, 184, 184, (一社)日本骨代謝学会, Jun. 2024
  Japanese
• テリパラチドによるイヌ皮質骨代謝への薬理作用解明を目的とした微細構造解析 地理情報システム(GIS)とAI駆動型形態認識システムの応用
  星 麻里絵 is an anti-osteoporotic drug with bone anabolic effects. Clinical and preclinical studies have indicated that TPTD has value in oral and maxillofacial bone therapies, including jawbone regeneration, periodontal tissue repair, and the treatment of medication-related osteonecrosis of the jaw. However, it is unclear whether the craniofacial bones respond to TPTD similarly to the axial and appendicular bones. Recent studies showed that TPTD acts on both osteocytes and osteoblasts. This study aimed to characterize distinct craniofacial bone sites, with a focus on morphometric changes in osteocytic lacunae in ovariectomized rats receiving TPTD. METHODS: Conventional bone histomorphometric analyses of mandibular and parietal bone sections were conducted. High-resolution confocal imaging-based three-dimensional fluorescence morphometric analyses of osteocytic lacunae in distinct mandibular and parietal bone sites were conducted. RESULTS: We observed dynamic changes in the morphometric characteristics of osteocytic lacunae specifically in alveolar and other mandibular bone sites upon TPTD administration. CONCLUSIONS: These findings suggest that osteocytes in mandibular bone (specifically, alveolar bone) have unique functional characteristics of osteocytic perilacunar remodeling.
• Evaluation of cortical bone remodeling in canines treated with daily and weekly administrations of teriparatide by establishing AI-driven morphometric analyses and GIS-based spatial mapping.
  Marie Hoshi-Numahata; Aya Takakura; Atsuko Nakanishi-Kimura; Haruhisa Watanabe; Kentaro Takada; Mai Nishiura; Yoshiaki Sato; Ryoko Takao-Kawabata; Tadahiro Iimura
  Bone reports, 19, 101720, 101720, Dec. 2023, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Larger animal models with a well-developed Haversian system, as observed in humans, are ideal to analyze cortical bone remodeling in pharmacological studies of anti-osteoporosis drugs, although they have some limitations in controlling individual variability in size, weight, age, and number. This study aimed to morphometrically analyze cortical bone remodeling focusing on Haversian canals in dogs using four regimens of TPTD with daily and weekly administrations at lower and higher weekly doses (4.9 μg/kg/week and 19.8 μg/kg/week, respectively) for 9 months. A micro-computed tomography-based analysis showed no significant differences among regimen groups. By establishing artificial intelligence (AI)-driven morphometric analyses and geographical information system (GIS)-based spatial mapping of Haversian canals that does not require confocal microscopy but is possible with more commonly used wide field microscopes, we successfully observed significant morphometric distinctions among regimens applied even in dogs. Our analytical results suggested that the daily higher regimen specifically increased the number of eroded pores creating spaces between existing canals, thus stimulating cortical bone remodeling.
• Centrosome clustering control in osteoclasts through CCR5-mediated signaling
  Ji-Won Lee; In-Hee Lee; Haruhisa Watanabe; Yunqing Liu; Kazuaki Sawada; Masashi Maekawa; Shunsuke Uehara; Yasuhiro Kobayashi; Yuuki Imai; Sek Won Kong; Tadahiro Iimura
  Scientific Reports, 13, 1, Springer Science and Business Media LLC, 27 Nov. 2023, [Peer-reviewed], [Last author, Corresponding author]
  Scientific journal, Abstract
  
  Osteoclasts uniquely resorb calcified bone matrices. To exert their function, mature osteoclasts maintain the cellular polarity and directional vesicle trafficking to and from the resorbing bone surface. However, the regulatory mechanisms and pathophysiological relevance of these processes remain largely unexplored. Bone histomorphometric analyses in Ccr5-deficient mice showed abnormalities in the morphology and functional phenotype of their osteoclasts, compared to wild type mice. We observed disorganized clustering of nuclei, as well as centrosomes that organize the microtubule network, which was concomitant with impaired cathepsin K secretion in cultured Ccr5-deficient osteoclasts. Intriguingly, forced expression of constitutively active Rho or Rac restored these cytoskeletal phenotypes with recovery of cathepsin K secretion. Furthermore, a gene-disease enrichment analysis identified that PLEKHM1, a responsible gene for osteopetrosis, which regulates lysosomal trafficking in osteoclasts, was regulated by CCR5. These experimental results highlighted that CCR5-mediated signaling served as an intracellular organizer for centrosome clustering in osteoclasts, which was involved in the pathophysiology of bone metabolism.
• 最新の歯学 「お酒に強い」と「骨が丈夫」の意外な関連性
  星 麻里絵[沼端]; 中西 徳子[木村]; 渡辺 陽久; 西浦 まい; 佐藤 嘉晃; 飯村 忠浩
  北海道歯学雑誌, 44, 10, 11, 北海道歯学会, Sep. 2023, [Last author, Corresponding author]
  Japanese
• 【顎関節の退行性病変におけるケモカインCCL5の役割とバイオマーカーとしての可能性】
  渡辺 陽久; 星 麻里絵; 西浦 まい; 丸岡 豊; 北川 善政; 飯村 忠浩
  北海道歯学雑誌, 44, 12, 15, 北海道歯学会, Sep. 2023, [Last author, Corresponding author]
  Japanese
• Skeletal-Vascular Interactions in Bone Development, Homeostasis, and Pathological Destruction.
  Haruhisa Watanabe; Nako Maishi; Marie Hoshi-Numahata; Mai Nishiura; Atsuko Nakanishi-Kimura; Kyoko Hida; Tadahiro Iimura
  International journal of molecular sciences, 24, 13, 30 Jun. 2023, [Peer-reviewed], [Invited], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Bone is a highly vascularized organ that not only plays multiple roles in supporting the body and organs but also endows the microstructure, enabling distinct cell lineages to reciprocally interact. Recent studies have uncovered relevant roles of the bone vasculature in bone patterning, morphogenesis, homeostasis, and pathological bone destruction, including osteoporosis and tumor metastasis. This review provides an overview of current topics in the interactive molecular events between endothelial cells and bone cells during bone ontogeny and discusses the future direction of this research area to find novel ways to treat bone diseases.
• 骨形態計測研究の新展開-新時代を拓く若手女性研究者たち CCR5を介した細胞の極性調節における中心体クラスター形成の役割(The role of centrosome clustering in CCR5-medaited cell polarity)
  李 智媛; 飯村 忠浩
  日本骨形態計測学会雑誌, 33, 1, 118, 118, 日本骨形態計測学会, May 2023, [Last author, Corresponding author]
  English
• AI駆動型形態計測を用いたイヌ皮質骨ハバース管リモデリングに対する骨形成促進薬テリパラチドの薬理作用
  星 麻里絵[沼端]; 高倉 綾; 中西 徳子[木村]; 李 智媛; 佐藤 嘉晃; 高尾 亮子; 飯村 忠浩
  日本骨形態計測学会雑誌, 33, 1, 172, 172, 日本骨形態計測学会, May 2023, [Last author, Corresponding author]
  Japanese
• Association between an Increased Serum CCL5 Level and Pathophysiology of Degenerative Joint Disease in the Temporomandibular Joint in Females.
  Haruhisa Watanabe; Takashi Iori; Ji-Won Lee; Takashi S Kajii; Aya Takakura; Ryoko Takao-Kawabata; Yoshimasa Kitagawa; Yutaka Maruoka; Tadahiro Iimura
  International journal of molecular sciences, 24, 3, 01 Feb. 2023, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Degenerative joint disease of the temporomandibular joints (DJD-TMJ) clinically manifests with symptoms such as orofacial pain, joint sounds and limited jaw movements. Our research group previously reported the functional necessity of a chemokine-chemokine receptor axis of CCL5-CCR5 in osteoclasts. Accumulated studies reported that this axis was involved in the pathogenesis of bone and joint destructive diseases, suggesting CCL5 as a potent biomarker. This study investigated whether or not the serum level of CCL5 can be a biomarker of DJD-TMJ and concomitantly analyzed changes in the serum and urine levels of bone markers to see whether or not changes in the rate of bone metabolism were predisposing. We enrolled 17 female subjects with diagnosed DJD-TMJ and sexually and age-matched 17 controls. The serum CCL5 level in DJD-TMJ subjects was significantly higher than that in the control subjects. Multivariate analyses indicated an association between an augmented CCL5 level and the rate of bone metabolism, especially in relatively young DJD-TMJ subjects without other systemic symptoms. A principal component analysis of serum markers and our pharmacological experiment using a postmenopausal model of ovariectomized rats suggested that an augmented serum CCL5 level specifically reflected DJD-TMJ and that covert changes in the rate of bone metabolism predisposed individuals to DJD-TMJ.
• Expansion of the osteocytic lacunar-canalicular system involved in pharmacological action of PTH revealed by AI-driven fluorescence morphometry in female rabbits.
  Aya Takakura; Takanori Sato; Ji-Won Lee; Kyoko Hirano; Ryoko Takao-Kawabata; Toshinori Ishizuya; Tadahiro Iimura
  Scientific reports, 12, 1, 16799, 16799, 07 Oct. 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Osteoporosis is an age-related disorder that is characterized by reduced bone mass. Its prevention and treatment are important healthcare issues for maintaining social activity in aged societies. Although bone fractures mostly occur at sites of weakened cortical bone, pathophysiological and pharmacological evaluations of bone mass have tended to be predominantly assessed in trabecular bone. To statistically characterize cortical bone remodeling, we originally established multimode fluorescence imaging and artificial intelligence (AI)-driven morphometric analyses in six-month-old female rabbits with well-defined cortical remodeling, similar to that in humans. We evaluated three distinct administration frequencies of teriparatide [TPTD; human parathyroid hormone, hPTH (1-34)]: once (1/w), twice (2/w), and seven times (7/w) a week, with the same total dose (140 μg/kg/week). Our analyses revealed significant expansions of the osteocytic lacunar-canalicular system and Haversian canals accompanied by the development of cortical porosity and endosteal naïve bone formation induced by a frequent administration regimen (7/w) of TPTD; however, once-weekly (1/w) and twice-weekly (2/w) administration of TPTD showed little effect. These findings demonstrate a clear contrast between the effects of frequent and infrequent administration of TPTD on cortical bone metabolism and suggest that osteocytic bone remodeling is involved in the pharmacological action of PTH.
• 最新の歯学 矯正治療時における骨メカノセンサーとしての骨細胞の役割
  中西 徳子[木村]; 星 麻里絵[沼端]; 佐藤 孝紀; 李 智媛; 佐藤 嘉晃; 飯村 忠浩
  北海道歯学雑誌, 43, 5, 8, 北海道歯学会, Sep. 2022, [Last author, Corresponding author]
  Japanese
• Nanoscale-tipped wire array injections transfer DNA directly into brain cells ex vivo and in vivo.
  Rika Numano; Akihiro Goryu; Yoshihiro Kubota; Hirohito Sawahata; Shota Yamagiwa; Minako Matsuo; Tadahiro Iimura; Hajime Tei; Makoto Ishida; Takeshi Kawano
  FEBS open bio, 12, 4, 835, 851, Apr. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Genetic modification to restore cell functions in the brain can be performed through the delivery of biomolecules in a minimally invasive manner into live neuronal cells within brain tissues. However, conventional nanoscale needles are too short (lengths of ~10 µm) to target neuronal cells in ~1-mm-thick brain tissues because the neuronal cells are located deep within the tissue. Here, we report the use of nanoscale-tipped wire (NTW) arrays with diameters < 100 nm and wire lengths of ~200 µm to address biomolecule delivery issues. The NTW arrays were manufactured by growth of silicon microwire arrays and nanotip formation. This technique uses pinpoint, multiple-cell DNA injections in deep areas of brain tissues, enabling target cells to be marked by fluorescent protein (FP) expression vectors. This technique has potential for use for electrophysiological recordings and biological transfection into neuronal cells. Herein, simply pressing an NTW array delivers and expresses plasmid DNA in multiple-cultured cells and multiple-neuronal cells within a brain slice with reduced cell damage. Additionally, DNA transfection is demonstrated using brain cells ex vivo and in vivo. Moreover, knockdown of a critical clock gene after injecting a short hairpin RNA (shRNA) and a genome-editing vector demonstrates the potential to genetically alter the function of living brain cells, for example, pacemaker cells of the mammalian circadian rhythms. Overall, our NTW array injection technique enables genetic and functional modification of living cells in deep brain tissue areas, both ex vivo and in vivo.
• Histone H3K27 demethylase, Utx, regulates osteoblast-to-osteocyte differentiation.
  Yuhan Xia; Aoi Ikedo; Ji-Won Lee; Tadahiro Iimura; Kazuki Inoue; Yuuki Imai
  Biochemical and biophysical research communications, 590, 132, 138, 29 Jan. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Osteocytes are master regulators of skeletal homeostasis. However, little is known about the molecular mechanism of their differentiation. Epigenetic regulations, especially H3K27me3 modification, play critical roles in cell differentiation. Here, we found that H3K27me3 in the loci of osteocyte-expressing genes decreased during osteocyte differentiation and that H3K27me3 demethylase, Utx, was bound to the loci of those genes. To investigate the physiological functions of Utx in vivo, we generated late osteoblast-to-osteocyte specific Utx knockout mice using Dmp1-cre mice (UtxΔOcy/ΔOcy). Micro CT analyses showed that UtxΔOcy/ΔOcy displayed osteopenic phenotypes with lower bone volume and trabecular number, and greater trabecular separation. Bone histomorphometric analysis showed that bone mineralization and formation were significantly lower in UtxΔOcy/ΔOcy. Furthermore, Dmp1 expression and the number of osteocytes were significantly decreased in UtxΔOcy/ΔOcy. These results suggest that Utx in Dmp1-expressing osteoblast/osteocyte positively regulates osteoblast-to-osteocyte differentiation through H3K27me3 modifications in osteocyte genes. Our results provide new insight into the molecular mechanism of osteocyte differentiation.
• The Lipid-Binding Defective Dynamin 2 Mutant in Charcot-Marie-Tooth Disease Impairs Proper Actin Bundling and Actin Organization in Glomerular Podocytes.
  Eriko Hamasaki; Natsuki Wakita; Hiroki Yasuoka; Hikaru Nagaoka; Masayuki Morita; Eizo Takashima; Takayuki Uchihashi; Tetsuya Takeda; Tadashi Abe; Ji-Won Lee; Tadahiro Iimura; Moin A Saleem; Naohisa Ogo; Akira Asai; Akihiro Narita; Kohji Takei; Hiroshi Yamada
  Frontiers in cell and developmental biology, 10, 884509, 884509, 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Dynamin is an endocytic protein that functions in vesicle formation by scission of invaginated membranes. Dynamin maintains the structure of foot processes in glomerular podocytes by directly and indirectly interacting with actin filaments. However, molecular mechanisms underlying dynamin-mediated actin regulation are largely unknown. Here, biochemical and cell biological experiments were conducted to uncover how dynamin modulates interactions between membranes and actin in human podocytes. Actin-bundling, membrane tubulating, and GTPase activities of dynamin were examined in vitro using recombinant dynamin 2-wild-type (WT) or dynamin 2-K562E, which is a mutant found in Charcot-Marie-Tooth patients. Dynamin 2-WT and dynamin 2-K562E led to the formation of prominent actin bundles with constant diameters. Whereas liposomes incubated with dynamin 2-WT resulted in tubule formation, dynamin 2-K562E reduced tubulation. Actin filaments and liposomes stimulated dynamin 2-WT GTPase activity by 6- and 20-fold, respectively. Actin-filaments, but not liposomes, stimulated dynamin 2-K562E GTPase activity by 4-fold. Self-assembly-dependent GTPase activity of dynamin 2-K562E was reduced to one-third compared to that of dynamin 2-WT. Incubation of liposomes and actin with dynamin 2-WT led to the formation of thick actin bundles, which often bound to liposomes. The interaction between lipid membranes and actin bundles by dynamin 2-K562E was lower than that by dynamin 2-WT. Dynamin 2-WT partially colocalized with stress fibers and actin bundles based on double immunofluorescence of human podocytes. Dynamin 2-K562E expression resulted in decreased stress fiber density and the formation of aberrant actin clusters. Dynamin 2-K562E colocalized with α-actinin-4 in aberrant actin clusters. Reformation of stress fibers after cytochalasin D-induced actin depolymerization and washout was less effective in dynamin 2-K562E-expressing cells than that in dynamin 2-WT. Bis-T-23, a dynamin self-assembly enhancer, was unable to rescue the decreased focal adhesion numbers and reduced stress fiber density induced by dynamin 2-K562E expression. These results suggest that the low affinity of the K562E mutant for lipid membranes, and atypical self-assembling properties, lead to actin disorganization in HPCs. Moreover, lipid-binding and self-assembly of dynamin 2 along actin filaments are required for podocyte morphology and functions. Finally, dynamin 2-mediated interactions between actin and membranes are critical for actin bundle formation in HPCs.
• 顎関節軟骨退行性疾患におけるバイオマーカーおよび病態機能の探索
  服部 倫寛; 北川 善政; 丸岡 豊; 飯村 忠浩
  日本口腔科学会雑誌, 70, 2, 149, 149, (NPO)日本口腔科学会, Jul. 2021, [Last author, Corresponding author]
  Japanese
• Effects of parathyroid hormone (PTH) and other osteoporosis drugs on osteoporotic pain
  Tomoya Tanaka; Ryoko; Takao-Kuwabata; Tadahiro Iimura
  Journal of Japanese Society for Bone Morphometry, 31, 2, 167, 173, 01 Jul. 2021, [Peer-reviewed], [Invited], [Last author, Corresponding author]
  Japanese, Scientific journal, 12943875;12943874
• A Quantitative Analysis of Bone Lamellarity and Bone Collagen Linearity Induced by Distinct Dosing and Frequencies of Teriparatide Administration in Ovariectomized Rats and Monkeys.
  Takanori Sato; Aya Takakura; Ji-Won Lee; Kazuaki Tokunaga; Haruka Matsumori; Ryoko Takao-Kawabata; Tadahiro Iimura
  Microscopy (Oxford, England), 08 Jun. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, The lamellar structure of bone, which endows biomechanical rigidity to support the host organism is observed in mammals, including humans. It is therefore essential to develop a quantitative analysis to evaluate the lamellarity of bone, which would especially useful for the pharmacological evaluation of anti-osteoporotic drugs. This study applied a current system for the semi-automatic recognition of fluorescence signals to the analysis of un-decalcified bone sections from rat and monkey specimens treated with teriparatide (TPTD). Our analyses on bone formation pattern and collagen topology indicated that TPTD augmented bone lamellarity and bone collagen linearity, which were possibly associated with the recovery of collagen crosslinking, thus endowing bone rigidity.
• Genetic variation analyses indicate conserved SARS-CoV-2-host interaction and varied genetic adaptation in immune-response factors in modern human evolution.
  Ji-Won Lee; In-Hee Lee; Takanori Sato; Sek Won Kong; Tadahiro Iimura
  Development, growth & differentiation, 63, 3, 219, 227, 17 Feb. 2021, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
  English, Scientific journal, Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 is a pandemic as of early 2020. Upon infection, SARS-CoV-2 attaches to its receptor of angiotensin-converting enzyme 2 (ACE2) on the surface of host cells and then is internalized into host cells via enzymatic machineries. This subsequently stimulates immune response factors. Since the host-immune response and severity of COVID-19 vary among individuals, genetic risk factors for severe COVID-19 cases have been investigated. Our research group recently conducted a survey of genetic variants among SARS-CoV-2-interacting molecules across populations, noting near absence of difference in allele frequency spectrum between populations in these genes. Recent genome-wide association studies have identified genetic risk factors for severe COVID-19 cases in a segment of chromosome 3 that involves six genes encoding three immune-regulatory chemokine receptors and another three molecules. The risk haplotype seemed to be inherited from Neanderthals, suggesting genetic adaptation against pathogens in modern human evolution. Therefore, SARS-CoV-2 uses highly conserved molecules as its virion interaction, whereas its immune-response appears to be genetically biased in individuals to some extent. We herein review the molecular process of SARS-CoV-2 infection with adding our further survey of genetic variants of its related immune effectors. We also discuss aspects of modern human evolution.
• Two macrophages, osteoclasts and microglia: from development to pleiotropy.
  Ji-Won Lee; In-Hee Lee; Tadahiro Iimura; Sek Won Kong
  Bone research, 9, 1, 11, 11, 10 Feb. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Tissue-resident macrophages are highly specialized to their tissue-specific microenvironments, activated by various inflammatory signals and modulated by genetic and environmental factors. Osteoclasts and microglia are distinct tissue-resident cells of the macrophage lineage in bone and brain that are responsible for pathological changes in osteoporosis and Alzheimer's disease (AD), respectively. Osteoporosis is more frequently observed in individuals with AD compared to the prevalence in general population. Diagnosis of AD is often delayed until underlying pathophysiological changes progress and cause irreversible damages in structure and function of brain. As such earlier diagnosis and intervention of individuals at higher risk would be indispensable to modify clinical courses. Pleiotropy is the phenomenon that a genetic variant affects multiple traits and the genetic correlation between two traits could suggest a shared molecular mechanism. In this review, we discuss that the Pyk2-mediated actin polymerization pathway in osteoclasts and microglia in bone and brain, respectively, is the horizontal pleiotropic mediator of shared risk factors for osteoporosis and AD.
• Changes in the intra- and peri-cellular sclerostin distribution in lacuno-canalicular system induced by mechanical unloading
  Ryuta Osumi; Ziyi Wang; Yoshihito Ishihara; Naoya Odagaki; Tadahiro Iimura; Hiroshi Kamioka
  Journal of Bone and Mineral Metabolism, Springer Science and Business Media LLC, 25 Aug. 2020, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, INTRODUCTION: Mechanical stimuli regulate Sclerostin (Scl), a negative regulator of bone formation, expression in osteocytes. However, the detailed Scl distribution in osteocytes in response to mechanical unloading remains unclear. MATERIALS AND METHODS: Twelve-week-old male rats were used. The sciatic and femoral nerves on the right side were excised as mechanical unloading treatment. A sham operation was performed on the left side. One week after neurotrauma, the bone density of the femora was evaluated by peripheral quantitative computed tomography, and immunofluorescence was performed in coronal sections of the femoral diaphysis. The mean fluorescence intensity and fluorescent profile of Scl from the marrow to the periosteal side were analyzed to estimate the Scl expression and determine to which side (marrow or periosteal) the Scl prefers to distribute in response to mechanical unloading. The most sensitive region indicated by the immunofluorescence results was further investigated by transmission electron microscopy (TEM) with immunogold staining to show the Scl expression changes in different subcellular structures. RESULTS: In femur distal metaphysis, neurotrauma-induced mechanical unloading significantly decreased the bone density, made the distribution of Scl closer to the marrow on the anterior and medial side, and increased the Scl expression only on the lateral side. TEM findings showed that only the expression of Scl in canaliculi was increased by mechanical unloading. CONCLUSIONS: Our results showed that even short-term mechanical unloading is enough to decrease bone density, and mechanical unloading not only regulated the Scl expression but also changed the Scl distribution in both the osteocyte network and subcellular structures.
• Teriparatide relieves ovariectomy-induced hyperalgesia in rats, suggesting the involvement of functional regulation in primary sensory neurons by PTH-mediated signaling.
  Tanaka T; Takao-Kawabata R; Takakura A; Shimazu Y; Nakatsugawa M; Ito A; Lee JW; Kawasaki; Iimura T
  Sci Rep., 10, 1, 5346, 24 Mar. 2020, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal, 12943875
• Zscan10 suppresses osteoclast differentiation by regulating expression of Haptoglobin.
  Yanagihara Y; Inoue K; Saeki N; Sawada Y; Yoshida S; Lee J; Iimura T; Imai Y
  Bone, 122, 93, 100, Feb. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Zinc finger and SCAN domain containing 10 (Zscan10) was identified as a novel transcription factor that is involved in osteoclast differentiation in our previous report. However, the biological functions of Zscan10 are not fully understood except its roles in the maintenance of genome stability and pluripotency of embryonic stem cells. Therefore, the purpose of this study was to clarify the function of Zscan10 in somatic cells, especially during osteoclast differentiation. First, Zscan10 KO RAW264 (KO) cells were established by genome editing using CRISPR/Cas9 and single cell sorting. Then, control (Ctrl) and KO cells were differentiated into osteoclasts by RANKL stimulation. We observed that TRAP activity and the expression levels of differentiation marker genes, such as Nfatc1, were significantly increased and the expression of inhibitory factors, such as Irf8, was decreased in KO cells compared to Ctrl cells. These results suggest that Zscan10 might regulate transcription of the genes that negatively control osteoclastogenesis. To understand gene expression profiles controlled by Zscan10, RNA-seq was performed and stringent analyses identified the haptoglobin gene (Hp) as a possible target of Zscan10. In addition, ChIP against Zscan10 revealed that Zscan10 could interact with its binding motif located near the Hp gene locus as well as the transcription start site of Hp, suggesting that Zscan10 can directly regulate transcription of Hp. Finally, to examine the effects of Hp on osteoclastogenesis, KO cells were treated with recombinant Hp (rHp). rHp treatment suppressed TRAP activity of KO cells without affecting cell viability. Furthermore, it has been reported that Hp KO mice exhibit decreased bone mass and increased osteoclast number. Importantly, hemolytic disease patients exhibited decreased serum level of Hp as well as low bone mineral density. Taken together, this study suggests that Zscan10 negatively regulates osteoclast differentiation through transcription of Hp.
• Raman Spectroscopic Analysis to Detect Reduced Bone Quality after Sciatic Neurectomy in Mice.
  Ishimaru Y; Oshima Y; Imai Y; Iimura T; Takanezawa S; Hino K; Miura H
  Molecules, 23, 23, pii: E3081, Nov. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Bone mineral density (BMD) is a commonly used diagnostic indicator for bone fracture risk in osteoporosis. Along with low BMD, bone fragility accounts for reduced bone quality in addition to low BMD, but there is no diagnostic method to directly assess the bone quality. In this study, we investigated changes in bone quality using the Raman spectroscopic technique. Sciatic neurectomy (NX) was performed in male C57/BL6J mice (NX group) as a model of disuse osteoporosis, and sham surgery was used as an experimental control (Sham group). Eight months after surgery, we acquired Raman spectral data from the anterior cortical surface of the proximal tibia. We also performed a BMD measurement and micro-CT measurement to investigate the pathogenesis of osteoporosis. Quantitative analysis based on the Raman peak intensities showed that the carbonate/phosphate ratio and the mineral/matrix ratio were significantly higher in the NX group than in the Sham group. There was direct evidence of alterations in the mineral content associated with mechanical properties of bone. To fully understand the spectral changes, we performed principal component analysis of the spectral dataset, focusing on the matrix content. In conclusion, Raman spectroscopy provides reliable information on chemical changes in both mineral and matrix contents, and it also identifies possible mechanisms of disuse osteoporosis.
• ラマン顕微鏡を用いた糖尿病モデルマウスの骨質の計測
  石丸 泰光; 大嶋 佑介; 今井 祐記; 飯村 忠浩; 高根沢 聡太; 日野 和典; 三浦 裕正
  日本整形外科学会雑誌, 92, 8, S2062, S2062, (公社)日本整形外科学会, Aug. 2018
  Japanese
• コラーゲン線維構築を阻害することで生じる骨細胞形態の変化
  橋本 真奈; 田畑 香織; 飯村 忠浩; 上岡 寛; 原 徹; 長岡 紀幸; 大嶋 佑介
  日本骨代謝学会学術集会プログラム抄録集, 36回, 169, 169, (一社)日本骨代謝学会, Jul. 2018
  Japanese
• PV1, a novel Plasmodium falciparum merozoite dense granule protein, interacts with exported protein in infected erythrocytes.
  Masayuki Morita; Hikaru Nagaoka; Edward H Ntege; Bernard N Kanoi; Daisuke Ito; Takahiro Nakata; Ji-Won Lee; Kazuaki Tokunaga; Tadahiro Iimura; Motomi Torii; Takafumi Tsuboi; Eizo Takashima
  Scientific reports, 8, 1, 3696, 3696, 27 Feb. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Upon invasion, Plasmodium falciparum exports hundreds of proteins across its surrounding parasitophorous vacuole membrane (PVM) to remodel the infected erythrocyte. Although this phenomenon is crucial for the parasite growth and virulence, elucidation of precise steps in the export pathway is still required. A translocon protein complex, PTEX, is the only known pathway that mediates passage of exported proteins across the PVM. P. falciparum Parasitophorous Vacuolar protein 1 (PfPV1), a previously reported parasitophorous vacuole (PV) protein, is considered essential for parasite growth. In this study, we characterized PfPV1 as a novel merozoite dense granule protein. Structured illumination microscopy (SIM) analyses demonstrated that PfPV1 partially co-localized with EXP2, suggesting the protein could be a PTEX accessory molecule. Furthermore, PfPV1 and exported protein PTP5 co-immunoprecipitated with anti-PfPV1 antibody. Surface plasmon resonance (SPR) confirmed the proteins' direct interaction. Additionally, we identified a PfPV1 High-affinity Region (PHR) at the C-terminal side of PTP5 where PfPV1 dominantly bound. SIM analysis demonstrated an export arrest of PTP5ΔPHR, a PTP5 mutant lacking PHR, suggesting PHR is essential for PTP5 export to the infected erythrocyte cytosol. The overall results suggest that PfPV1, a novel dense granule protein, plays an important role in protein export at PV.
• Detection of changes in bone quality of osteoporotic model induced by sciatic nerve resection by using Raman spectroscopy
  Yasumitsu Ishimaru; Yusuke Oshima; Yuuki Imai; Tadahiro Iimura; Sota Takanezawa; Kazunori Hino; Hiromasa Miura
  Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 10497, SPIE, 2018, [Peer-reviewed]
  English, International conference proceedings
• 骨組織の顕微鏡研究.
  飯村忠浩; 李 智媛
  顕微鏡, 53, 1, 24, 28, (公社)日本顕微鏡学会, 2018, [Peer-reviewed], [Invited]
  Japanese
• Higher Frequencies of Teriparatide develops Cortical Porosity.
  高倉綾; 高倉綾; LEE Ji-Won; 山根宏志; 高尾亮子; 飯村忠浩; 飯村忠浩; 飯村忠浩; 飯村忠浩
  日本骨形態計測学会雑誌, 28, 1, 31, 37, 日本骨形態計測学会, 2018, [Peer-reviewed], [Invited]
  Japanese
• Uhrf1 is indispensable for normal limb growth by regulating chondrocyte differentiation through specific gene expression
  Michiko Yamashita; Kazuki Inoue; Noritaka Saeki; Maky Ideta-Otsuka; Yuta Yanagihara; Yuichiro Sawada; Iori Sakakibara; Jiwon Lee; Koichi Ichikawa; Yoshiaki Kamei; Tadahiro Iimura; Katsuhide Igarashi; Yasutsugu Takada; Yuuki Imai
  Development (Cambridge), 145, 1, dev157412, Company of Biologists Ltd, 01 Jan. 2018, [Peer-reviewed]
  English, Scientific journal
• ラマン顕微鏡を用いた坐骨神経切除マウスの骨質の計測
  石丸 泰光; 日野 和典; 三浦 裕正; 大嶋 佑介; 高根沢 聡太; 今井 祐記; 飯村 忠浩
  愛媛医学, 36, 4, 253, 253, 愛媛医学会, Dec. 2017
  Japanese
• The HIV co-receptor CCR5 regulates osteoclast function
  Ji-Won Lee; Akiyoshi Hoshino; Kazuki Inoue; Takashi Saitou; Shunsuke Uehara; Yasuhiro Kobayashi; Satoshi Ueha; Kouji Matsushima; Akira Yamaguchi; Yuuki Imai; Tadahiro Iimura
  NATURE COMMUNICATIONS, 8, 1, 2226, Dec. 2017, [Peer-reviewed]
  English, Scientific journal
• Bone Formation Is Coupled to Resorption Via Suppression of Sclerostin Expression by Osteoclasts
  Masanori Koide; Yasuhiro Kobayashi; Teruhito Yamashita; Shunsuke Uehara; Midori Nakamura; B. Yukihiro Hiraoka; Yuki Ozaki; Tadahiro Iimura; Hisataka Yasuda; Naoyuki Takahashi; Nobuyuki Udagawa
  JOURNAL OF BONE AND MINERAL RESEARCH, 32, 10, 2074, 2086, Oct. 2017, [Peer-reviewed]
  English, Scientific journal
• 骨疾患の病態解明と新たな治療法開発への基礎的アプローチ マウスジェネティクスを用いたHIV感染患者の骨病態解明
  飯村 忠浩; 李 智媛
  Journal of Oral Biosciences Supplement, 2017, 111, 111, (一社)歯科基礎医学会, Sep. 2017
  Japanese
• ラマン顕微鏡を用いた骨粗鬆症モデルマウスにおける骨質変化の測定
  石丸 泰光; 大嶋 佑介; 今井 祐記; 飯村 忠浩; 高根沢 聡太; 日野 和典; 三浦 裕正
  中国・四国整形外科学会雑誌, 29, 3, 412, 412, 中国・四国整形外科学会, Sep. 2017
  Japanese
• Osteocyte Biologyアップデート 骨細胞ネットワーク形成に与えるコラーゲン線維構築の関与
  上岡 寛; 橋本 真奈; 長岡 紀幸; 大嶋 佑介; 飯村 忠浩; 原 徹
  Journal of Oral Biosciences Supplement, 2017, 148, 148, (一社)歯科基礎医学会, Sep. 2017
  Japanese
• 破骨細胞由来のLIFはsclerostinの発現低下を介して、骨形成を促進する
  小出 雅則; 小林 泰浩; 山下 照仁; 上原 俊介; 中村 美どり; 平岡 行博; 尾崎 友輝; 飯村 忠浩; 高橋 直之; 宇田川 信之
  Journal of Oral Biosciences Supplement, 2017, 239, 239, (一社)歯科基礎医学会, Sep. 2017
  Japanese
• ラマン顕微鏡を用いた骨粗鬆症モデルマウスにおける骨質の評価
  石丸 泰光; 大嶋 佑介; 今井 祐記; 飯村 忠浩; 日野 和典; 三浦 裕正
  中部日本整形外科災害外科学会雑誌, 60, 秋季学会, 145, 145, (一社)中部日本整形外科災害外科学会, Sep. 2017
  Japanese
• ラマン顕微鏡を用いた骨粗鬆症モデルマウスの骨質の計測
  石丸 泰光; 大嶋 佑介; 今井 祐記; 飯村 忠浩; 高根沢 聡太; 日野 和典; 三浦 裕正
  日本整形外科学会雑誌, 91, 8, S1566, S1566, (公社)日本整形外科学会, Aug. 2017
  Japanese
• 電子顕微鏡・光学顕微鏡による最先端・骨イメージング 直交配置型FIB-SEMでみる、骨細胞ネットワーク形成とコラーゲン細線維構築の関与
  橋本 真奈; 長岡 紀幸; 大嶋 佑介; 飯村 忠浩; 原 徹; 上岡 寛
  日本骨形態計測学会雑誌, 27, 1, S58, S58, 日本骨形態計測学会, May 2017
  Japanese
• 電子顕微鏡・光学顕微鏡による最先端・骨イメージング ラマン分光・イメージングによる骨粗鬆症モデルの骨質評価
  大嶋 佑介; 石丸 泰光; 三浦 裕正; 今井 祐記; 飯村 忠浩
  日本骨形態計測学会雑誌, 27, 1, S59, S59, 日本骨形態計測学会, May 2017
  Japanese
• Acute development of cortical porosity and endosteal naive bone formation from the daily but not weekly short-term administration of PTH in rabbit
  Hiroshi Yamane; Aya Takakura; Yukari Shimadzu; Toshiyuki Kodama; Ji-Won Lee; Yukihiro Isogai; Toshinori Ishizuya; Ryoko Takao-Kawabata; Tadahiro Iimura
  PLOS ONE, 12, 4, e0175329, Apr. 2017, [Peer-reviewed]
  English, Scientific journal
• Administration frequency as well as dosage of PTH are associated with development of cortical porosity in ovariectomized rats
  Aya Takakura; Ji-Won Lee; Kyoko Hirano; Yukihiro Isogai; Toshinori Ishizuya; Ryoko Takao-Kawabata; Tadahiro Iimura
  BONE RESEARCH, 5, 17002, Apr. 2017, [Peer-reviewed]
  English, Scientific journal
• Shedding quantitative fluorescence light on novel regulatory mechanisms in skeletal biomedicine and biodentistry
  Ji-Won Lee; Tadahiro Iimura
  Japanese Dental Science Review, 53, 1, 2, 10, Elsevier Ltd, 01 Feb. 2017, [Peer-reviewed], [Invited]
  English
• Evaluation of bone quality in osteoporosis model mice by Raman spectroscopy
  Yasumitsu Ishimaru; Yusuke Oshima; Yuuki Imai; Tadahiro Iimura; Sota Takanezawa; Kazunori Hino; Hiromasa Miura
  Proceedings of SPIE - The International Society for Optical Engineering, 10251, SPIE, 2017, [Peer-reviewed]
  English, International conference proceedings
• Short-term intermittent administration of parathyroid hormone facilitates osteogenesis by different mechanisms in cancellous and cortical bone
  Kenji Ogura; Tadahiro Iimura; Yuji Makino; Ayano Sugie-Oya; Aya Takakura; Ryoko Takao-Kawabata; Toshinori Ishizuya; Keiji Moriyama; Akira Yamaguchi
  Bone Reports, 5, 7, 14, Elsevier Inc., 01 Dec. 2016, [Peer-reviewed]
  English, Scientific journal
• 歯科医学の基礎と臨床をつなぐ三次元超微形態学の新たな可能性 骨細胞ネットワーク形成に与えるコラーゲン線維集束化の関与
  橋本 真奈; 長岡 紀幸; 飯村 忠浩; 大嶋 佑介; 原 徹; 上岡 寛
  Journal of Oral Biosciences Supplement, 2016, 103, 104, (一社)歯科基礎医学会, Sep. 2016
  Japanese
• 破骨細胞の構造学的な機能におけるケモカインを介したシグナル伝達系の役割(Rising Stars in Skeletal Biology Roles of Chemokine-mediated signaling in architectural function of osteoclasts)
  李 智媛; 星野 昭芳; 井上 和樹; 上原 俊介; 小林 泰浩; 山口 朗; 今井 祐記; 飯村 忠浩
  日本骨代謝学会学術集会プログラム抄録集, 34回, 130, 130, (一社)日本骨代謝学会, Jul. 2016
  English
• [Bone Cell Biology Assessed by Microscopic Approach. An application of two-photon microscopy for histopathological analysis of bone and cartilage].
  Iimura T
  Clinical calcium, 25, 10, 1513, 1520, Oct. 2015, [Peer-reviewed]
• 【筋と骨】 筋・骨格系の統合的な発生・発達におけるメカニカルストレスとHox遺伝子群
  飯村 忠浩
  THE BONE, 29, 3, 265, 271, (株)メディカルレビュー社, Oct. 2015
  Japanese
• 骨細胞の機能と形態のヘテロジェニティ
  李 智媛; 飯村 忠浩
  THE BONE, 29, 3, 213, 218, (株)メディカルレビュー社, Oct. 2015
  Japanese
• 二光子励起顕微鏡を用いた軟骨変性の評価
  清松 悠; 大嶋 佑介; 斎藤 卓; 疋田 温彦; 三浦 裕正; 宮崎 剛; 飯村 忠浩; 今村 健志
  日本整形外科学会雑誌, 89, 8, S1455, S1455, (公社)日本整形外科学会, Sep. 2015
  Japanese
• 骨吸収の促進は骨細胞におけるsclerostinの発現を低下させ、Wnt/β-cateninシグナルを促進する
  小出 雅則; 小林 泰浩; 山下 照仁; 上原 俊介; 飯村 忠浩; 中村 美どり; 高橋 直之; 宇田川 信之; 保田 尚孝
  Journal of Oral Biosciences Supplement, 2015, 228, 228, (一社)歯科基礎医学会, Sep. 2015
  Japanese
• 【顕微学的アプローチによる骨の細胞機能の解明】 軟骨・骨の組織病理学的解析における2光子励起顕微鏡の応用
  飯村 忠浩
  Clinical Calcium, 25, 10, 1513, 1520, (株)医薬ジャーナル社, Sep. 2015
  Japanese
• 各種顕微鏡の進歩 さまざまな顕微鏡の革新的技術開発が拓く次世代多次元イメージング(第5回) 超解像顕微鏡
  飯村 忠浩
  病理と臨床, 33, 8, 893, 898, (株)文光堂, Aug. 2015
  Japanese
• 骨関連細胞ネットワークin vitro再構築系の2光子励起顕微鏡による解析
  疋田 温彦; 飯村 忠浩; 大嶋 佑介; 今村 健志
  日本骨代謝学会学術集会プログラム抄録集, 33回, 161, 161, (一社)日本骨代謝学会, Jul. 2015
  Japanese
• Analyses of bone modeling and remodeling using in vitro reconstitution system with two-photon microscopy
  Atsuhiko Hikita; Tadahiro Iimura; Yusuke Oshima; Takashi Saitou; Shin Yamamoto; Takeshi Imamura
  BONE, 76, 5, 17, Jul. 2015, [Peer-reviewed]
  English, Scientific journal
• Oral mucosal fibroblasts overexpressing BMP-2 differentiate into osteoblasts and participate in new bone formation during bone regeneration
  Lin Zayar; Iimura Tadahiro; Kasugai Shohei; Yamaguchi Akira
  JOURNAL OF ORAL BIOSCIENCES, 57, 2, 118, 123, May 2015, [Peer-reviewed]
• Quantitative in situ fluorescence imaging to unveil the morphological and functional heterogeneity of osteocytes
  Ji-Won Lee; Tadahiro Iimura
  Journal of Oral Biosciences, 57, 2, 76, 79, Japanese Association for Oral Biology, 01 May 2015, [Peer-reviewed]
  English, Scientific journal
• Oral mucosal fibroblasts overexpressing BMP-2 differentiate into osteoblasts and participate in new bone formation during bone regeneration
  Zayar Lin; Tadahiro Iimura; Shohei Kasugai; Akira Yamaguchi
  Journal of Oral Biosciences, 57, 2, 118, 123, Japanese Association for Oral Biology, 01 May 2015, [Peer-reviewed]
  English, Scientific journal
• 骨細胞のin situ蛍光計測による機能解析
  飯村 忠浩
  O.li.v.e., 5, 2, 123, 123, (株)メディカルレビュー社, May 2015
  Japanese
• Rosmarinic acid exerts an antiosteoporotic effect in the RANKL-induced mouse model of bone loss by promotion of osteoblastic differentiation and inhibition of osteoclastic differentiation
  Ji-Won Lee; Midori Asai; Sang-Kyung Jeon; Tadahiro Iimura; Takayuki Yonezawa; Byung-Yoon Cha; Je-Tae Woo; Akira Yamaguchi
  MOLECULAR NUTRITION & FOOD RESEARCH, 59, 3, 386, 400, Mar. 2015, [Peer-reviewed]
  English, Scientific journal
• Hox genes control vertebrate body elongation by collinear Wnt repression
  Nicolas Denans; Tadahiro Iimura; Olivier Pourquie
  ELIFE, 4, Feb. 2015, [Peer-reviewed]
  English, Scientific journal
• Quantitative SHG imaging in osteoarthritis model mice, implying a diagnostic application
  Hiroshi Kiyomatsu; Yusuke Oshima; Takashi Saitou; Tsuyoshi Miyazaki; Atsuhiko Hikita; Hiromasa Miura; Tadahiro Iimura; Takeshi Imamura
  BIOMEDICAL OPTICS EXPRESS, 6, 2, 405, 420, Feb. 2015, [Peer-reviewed]
  English, Scientific journal
• In vivo detection of cancer cells with immunoconjugated fluorescent probes by macro zoom microscopy and two-photon microscopy
  Shigehiro Koga; Yusuke Oshima; Atsuhiko Hikita; Koichi Sato; Motohira Yoshida; Yuji Yamamoto; Tadahiro Iimura; Yuji Watanabe; Takeshi Imamura
  Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 9339, SPIE, 2015, [Peer-reviewed]
  English, International conference proceedings
• Changes in chemical composition of bone matrix in ovariectomized (OVX) rats detected by Raman spectroscopy and multivariate analysis
  Yusuke Oshima; Tadahiro Iimura; Takashi Saitou; Takeshi Imamura
  PHOTONIC THERAPEUTICS AND DIAGNOSTICS XI, 9303, 2015, [Peer-reviewed]
  English, International conference proceedings
• 超域ネットワーク型｣生命医学研究支援体制の構築に向けて.
  飯村 忠浩
  愛媛ジャーナル, 29, 75, 77, 2015, [Invited]
  Japanese, Scientific journal
• Changes in the spatial distribution of sclerostin in the osteocytic lacuno-canalicular system in alveolar bone due to orthodontic forces, as detected on multimodal confocal fluorescence imaging analyses
  Yuriko Nishiyama; Tsutomu Matsumoto; Ji-Won Lee; Takashi Saitou; Takeshi Imamura; Keiji Moriyama; Akira Yamaguchi; Taclahiro Iimura
  ARCHIVES OF ORAL BIOLOGY, 60, 1, 45, 54, Jan. 2015, [Peer-reviewed]
  English, Scientific journal
• Changes in chemical composition of bone matrix in ovariectomized (OVX) rats detected by Raman spectroscopy and multivariate analysis
  Yusuke Oshima; Tadahiro Iimura; Takashi Saitou; Takeshi Imamura
  Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 9303, SPIE, 2015
  English, International conference proceedings
• Live Imaging-Based Model Selection Reveals Periodic Regulation of the Stochastic G1/S Phase Transition in Vertebrate Axial Development
  Mayu Sugiyama; Takashi Saitou; Hiroshi Kurokawa; Asako Sakaue-Sawano; Takeshi Imamura; Atsushi Miyawaki; Tadahiro Iimura
  PLOS COMPUTATIONAL BIOLOGY, 10, 12, e1003957, Dec. 2014, [Peer-reviewed]
  English, Scientific journal
• In vivo subcellular imaging of tumors in mouse models using a fluorophore-conjugated anti-carcinoembryonic antigen antibody in two-photon excitation microscopy
  Shigehiro Koga; Yusuke Oshima; Naoki Honkura; Tadahiro Iimura; Kenji Kameda; Koichi Sato; Motohira Yoshida; Yuji Yamamoto; Yuji Watanabe; Atsuhiko Hikita; Takeshi Imamura
  CANCER SCIENCE, 105, 10, 1299, 1306, Oct. 2014, [Peer-reviewed]
  English, Scientific journal
• 骨代謝研究領域におけるバイオイメージング技術の発展からその応用まで ラマン分光・イメージング技術の開発と骨代謝研究への応用
  大嶋 佑介; 飯村 忠浩; 疋田 温彦; 今村 健志
  日本骨代謝学会学術集会プログラム抄録集, 32回, 161, 161, (一社)日本骨代謝学会, Jul. 2014
  Japanese
• 【Molecular Imaging 2014 分子イメージングはどこまで進んだか】 分子イメージングの最新動向 光イメージングの最新動向 バイオイメージングの現状と展望
  今村 健志; 疋田 温彦; 大嶋 佑介; 飯村 忠浩
  INNERVISION, 29, 7, 39, 41, (株)インナービジョン, Jun. 2014
  Japanese
• 【骨バイオイメージング】 次世代骨バイオイメージング ラマン分光法による骨組織の分子計測と骨質評価への応用
  大嶋 佑介; 飯村 忠浩; 疋田 温彦; 今村 健志
  THE BONE, 28, 2, 197, 202, (株)メディカルレビュー社, Jun. 2014
  Japanese
• 【骨バイオイメージング】 次世代骨バイオイメージング 2光子励起顕微鏡を用いた骨・軟骨組織イメージング
  疋田 温彦; 大嶋 佑介; 飯村 忠浩; 今村 健志
  THE BONE, 28, 2, 191, 195, (株)メディカルレビュー社, Jun. 2014
  Japanese
• 【骨バイオイメージング】 静的イメージング 骨細胞の3次元解析
  飯村 忠浩
  THE BONE, 28, 2, 165, 172, (株)メディカルレビュー社, Jun. 2014
  Japanese
• 医歯工連携シンポジウム 画像イメージングと骨形態計測の接点 骨・軟骨組織の2光子励起顕微鏡・第2次高調波発生を用いた観察
  疋田 温彦; 大嶋 佑介; 清松 悠; 飯村 忠浩; 今村 健志
  日本骨形態計測学会雑誌, 24, 2, S53, S53, 日本骨形態計測学会, May 2014
  Japanese
• 医歯工連携シンポジウム 画像イメージングと骨形態計測の接点 ラマン分光分析を基盤とした骨質計測技術の開発
  大嶋 佑介; 飯村 忠浩; 疋田 温彦; 今村 健志
  日本骨形態計測学会雑誌, 24, 2, S54, S54, 日本骨形態計測学会, May 2014
  Japanese
• 【骨代謝 つくり、壊し、変える-そのメカニズムと最新治療 分子機構から骨粗鬆症・リウマチなど骨疾患への応用まで】(第I部) 骨代謝制御機構を知る (第3章)骨をみる イメージングの現状と展望 骨細胞の形態と機能ダイナミズムの可視化・定量化
  飯村 忠浩
  実験医学, 32, 7, 1067, 1073, (株)羊土社, May 2014
  Japanese
• 蛍光生体4Dイメージングが拓く新たな形態学
  今村 健志; 疋田 温彦; 飯村 忠浩; 大嶋 佑介
  日本病理学会会誌, 103, 1, 152, 152, (一社)日本病理学会, Mar. 2014
  Japanese
• A possible role of DMP1 as a negative regulator of FGF23 production in functional heterogeneity osteocytes: Three-dimensional morphological approaches.
  Ji-Won Lee; Akira Yamaguchi; Tadahiro Iimura
  JOURNAL OF BONE AND MINERAL RESEARCH, 29, S171, S171, Feb. 2014, [Peer-reviewed]
  English
• Functional heterogeneity of osteocytes in FGF23 production: the possible involvement of DMP1 as a direct negative regulator.
  Lee JW; Yamaguchi A; Iimura T
  BoneKEy reports, 3, 543, 543, 2014, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Fibroblast growth factor 23 (FGF23) and dentin matrix protein (DMP1) are hallmarks of osteocytes in bone. However, the mechanisms underlying the actions of DMP1 as a local factor regulating FGF23 and bone mineralization are not well understood. We first observed spatially distinct distributions of FGF23- and DMP1-positive osteocytic lacunae in rat femurs using immunohistochemistry. Three-dimensional immunofluorescence morphometry further demonstrated that the distribution and relative expression levels of these two proteins exhibited reciprocally reversed patterns especially in midshaft cortical bone. These in vivo findings suggest a direct role of DMP1 in FGF23 expression in osteocytes. We next observed that the inoculation of recombinant DMP1 in UMR-106 osteoblast/osteocyte-like cells and long-cultured MC3T3-E1 osteoblastic cells showed significant downregulation of FGF23 production. This effect was rescued by incubation with an focal adhesion kinase (FAK) inhibitor or MEK (mitogen-activated protein kinase (MAPK)/extracellular signal regulated kinase (ERK)) inhibitor but not inhibitors of phosphoinositide 3-kinase or Rho kinase. Consistently, the levels of phosphorylated FAK, ERK and p38 were significantly elevated, indicating that exogenous DMP1 is capable of activating FAK-mediated MAPK signaling. These findings suggest that DMP1 is a local, direct and negative regulator of FGF23 production in osteocytes involved in the FAK-mediated MAPK pathway, proposing a relevant pathway that coordinates the extracellular environment of osteocytic lacunae and bone metabolism.
• [Intravital optical imaging of bone and cartilage tissues].
  Imamura T; Hikita A; Oshima Y; Iimura T
  Clinical calcium, 23, 12, 1767, 1773, Dec. 2013, [Peer-reviewed]
• 【骨・軟骨疾患と再生】 骨・軟骨組織の生体光イメージング
  今村 健志; 疋田 温彦; 大嶋 佑介; 飯村 忠浩
  Clinical Calcium, 23, 12, 1767, 1773, (株)医薬ジャーナル社, Nov. 2013
  Japanese
• 【骨細胞:骨を制御する司令塔】 骨細胞ネットワークのイメージング
  飯村 忠浩
  THE BONE, 27, 3, 271, 277, (株)メディカルレビュー社, Aug. 2013
  Japanese
• Retention of bone strength by feeding of Milk and dairy products in ovariectomized rats: involvement of changes in serum levels of 1alpha, 25(OH)(2)D-3 and FGF23
  Rieko Tanabe; Mayu Haraikawa; Natsuko Sogabe; Aoi Sugimoto; Yuka Kawamura; Satoshi Takasugi; Masashi Nagata; Ayako Nakane; Akira Yamaguchi; Tadahiro Iimura; Masae Goseki-Sone
  JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 24, 6, 1000, 1007, Jun. 2013, [Peer-reviewed]
  English, Scientific journal
• RANKL synthesized by both stromal cells and cancer cells plays a crucial role in osteoclastic bone resorption induced by oral cancer.
  Kiyoshi Sato; Ji-Won Lee; Kei Sakamoto; Tadahiro Iimura; Kou Kayamori; Hisataka Yasuda; Masanobu Shindoh; Masako Ito; Ken Omura; Akira Yamaguchi
  Am. J. Pathol., 182, 5, 1890, 1899, May 2013, [Peer-reviewed]
  English, The molecular mechanisms underlying bone destruction by invading oral cancer are not well understood. Using IHC, we demonstrated that receptor activator of nuclear factor-κB ligand (RANKL)-positive fibroblasts and cancer cells were located at sites of bone invasion in human oral cancers. HSC3 and HO-1-N-1, human oral cancer cell lines, expressed RANKL and stimulated Rankl expression in the UAMS-32 murine osteoblastic cell line. We discriminated the roles of RANKL synthesized by stromal cells and cancer cells in cancer-associated bone resorption by using species-specific RANKL antibodies against murine RANKL and human RANKL, respectively. Osteoclastogenesis induced by the conditioned medium of HSC3 and HO-1-N-1 cells in a co-culture of murine bone marrow cells and UAMS-32 cells was inhibited by the addition of antibodies against either mouse or human RANKL. HSC3-induced bone destruction was greatly inhibited by the administration of anti-mouse RANKL antibody in a xenograft model. HO-1-N-1-induced bone destruction was inhibited by the administration of either anti-mouse or anti-human RANKL antibody. Bone destruction induced by the transplantation of human RANKL-overexpressing cells
• RANKL Synthesized by Both Stromal Cells and Cancer Cells Plays a Crucial Role in Osteoclastic Bone Resorption Induced by Oral Cancer
  Kiyoshi Sato; Ji-Won Lee; Kei Sakamoto; Tadahiro Iimura; Kou Kayamori; Hisataka Yasuda; Masanobu Shindoh; Masako Ito; Ken Omura; Akira Yamaguchi
  AMERICAN JOURNAL OF PATHOLOGY, 182, 5, 1890, 1899, May 2013, [Peer-reviewed]
  English, Scientific journal
• The role of osteocytes in bone resorption during orthodontic tooth movement
  T. Matsumoto; T. Iimura; K. Ogura; K. Moriyama; A. Yamaguchi
  Journal of Dental Research, 92, 4, 340, 345, Apr. 2013, [Peer-reviewed]
  English, Scientific journal
• Visualization of an endogenous retinoic acid gradient across embryonic development
  Satoshi Shimozono; Tadahiro Iimura; Tetsuya Kitaguchi; Shin-ichi Higashijima; Atsushi Miyawaki
  NATURE, 496, 7445, 363, +, Apr. 2013, [Peer-reviewed]
  English, Scientific journal
• Spatiotemporal disorder in the axial skeleton development of the Mesp2-null mouse: A model of spondylocostal dysostosis and spondylothoracic dysostosis
  Yuji Makino; Yu Takahashi; Rieko Tanabe; Yoshihiro Tamamura; Takashi Watanabe; Mayu Haraikawa; Miwako Hamagaki; Kenji Hata; Jun Kanno; Toshiyuki Yoneda; Yumiko Saga; Masae Goseki-Sone; Kazuo Kaneko; Akira Yamaguchi; Tadahiro Iimura
  BONE, 53, 1, 248, 258, Mar. 2013, [Peer-reviewed]
  English, Scientific journal
• 生後骨発達におけるSclerostin発現分布の時空間的変化 蛍光イメージングによる骨組織のグローカルな観察
  渡辺 高; 原田 清; 山口 朗; 飯村 忠浩
  THE BONE, 27, 1, 5, 10, (株)メディカルレビュー社, Mar. 2013
  Japanese
• Roles of chemokine receptor CX3CR1 in maintaining murine bone homeostasis through the regulation of both osteoblasts and osteoclasts
  Akiyoshi Hoshino; Satoshi Ueha; Sanshiro Hanada; Toshio Imai; Masako Ito; Kenji Yamamoto; Kouji Matsushima; Akira Yamaguchi; Tadahiro Iimura
  JOURNAL OF CELL SCIENCE, 126, 4, 1032, 1045, Feb. 2013, [Peer-reviewed]
  English, Scientific journal
• Developmental biology and etiology of axial skeleton: Lessons from a mouse model of spondylocostal dysostosis and spondylothoracic dysostosis
  Yuji Makinoa; Kazuo Kanekob; Akira Yamaguchia; Tadahiro Iimura
  Journal of Oral Biosciences, 55, 4, 175, 179, Japanese Association for Oral Biology, 2013, [Peer-reviewed]
  English
• 進行性/特発性下顎頭吸収におけるケモカイン受容体異常により生じる骨/軟骨減少に関する研究(Study of Osteo-/Chondropenia Caused by Impaired Chemokine Receptor and for Progressive/Idiopathic Condylar Resorption)
  Maruoka Yutaka; Kanaya Fumihide; Hoshino Akiyoshi; Iimura Tadahiro; Imai Hideki; Otsuka Ryo; Ueha Satoshi; Fujioka Kohki; Katsuragawa Yozo; Shimbo Takuro; Mimori Akio; Yamazaki Tsutomu; Manome Yoshinobu; Moriyama Keiji; Omura Ken; Matsushima Kouji; Yamamoto Kenji
  日本顎変形症学会雑誌, 22, 補冊, S15, S22, (NPO)日本顎変形症学会, Dec. 2012, [Peer-reviewed]
  English, 日本国内の進行性下顎頭吸収(PCR)の179症例を検討した。自覚症状を伴わないPCR患者が多くみられた。患者の一部は咬合不調和のため口腔外科よりも矯正歯科を受診していた。患者の多くを女性が占めた。PCR患者は特発性の若年患者と自己免疫疾患などの合併症がある50代以上の患者の二峰性分布を示した。PCR患者の主訴として顎関節症よりも咬合不調和が多かった。一側性よりも両側性障害が多かった。PCR患者の初診時の診断の多くは顎関節症であり、両疾患の判別の困難さが示唆された。PCRの治療は顎関節症に対するものと同様であった。単純な顎関節症、顎変形や咬合異常と診断され、適切な治療を受けていないPCR患者の存在が示唆された。
• Increasing participation of sclerostin in postnatal bone development, revealed by three-dimensional immunofluorescence morphometry
  Takashi Watanabe; Yoshihiro Tamamura; Akiyoshi Hoshino; Yuji Makino; Hiroshi Kamioka; Teruo Amagasa; Akira Yamaguchi; Tadahiro Iimura
  BONE, 51, 3, 447, 458, Sep. 2012, [Peer-reviewed]
  English, Scientific journal
• Canine Oral Mucosal Fibroblasts Differentiate into Osteoblastic Cells in Response to BMP-2
  Kohsuke Umehara; Tadahiro Iimura; Kei Sakamoto; Zayar Lin; Shohei Kasugai; Yoshimasa Igarashi; Akira Yamaguchi
  ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY, 295, 8, 1327, 1335, Aug. 2012, [Peer-reviewed]
  English, Scientific journal
• CXCL2 synthesized by oral squamous cell carcinoma is involved in cancer-associated bone destruction
  Erika Oue; Ji-Won Lee; Kei Sakamoto; Tadahiro Iimura; Kazuhiro Aoki; Kou Kayamori; Yasuyuki Michi; Masashi Yamashiro; Kiyoshi Harada; Teruo Amagasa; Akira Yamaguchi
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 424, 3, 456, 461, Aug. 2012, [Peer-reviewed]
  English, Scientific journal
• A fluorescence spotlight on the clockwork development and metabolism of bone
  Tadahiro Iimura; Ayako Nakane; Mayu Sugiyama; Hiroki Sato; Yuji Makino; Takashi Watanabe; Yuzo Takagi; Rika Numano; Akira Yamaguchi
  JOURNAL OF BONE AND MINERAL METABOLISM, 30, 3, 254, 269, May 2012, [Peer-reviewed]
  English
• Cdc42 is required for chondrogenesis and interdigital programmed cell death during limb development
  Ryo Aizawa; Atsushi Yamada; Dai Suzuki; Tadahiro Iimura; Hidetoshi Kassai; Takeshi Harada; Masayuki Tsukasaki; Gou Yamamoto; Tetsuhiko Tachikawa; Kazuki Nakao; Matsuo Yamamoto; Akira Yamaguchi; Atsu Aiba; Ryutaro Kamijo
  MECHANISMS OF DEVELOPMENT, 129, 1-4, 38, 50, Mar. 2012, [Peer-reviewed]
  English, Scientific journal
• Structural differences in the osteocyte network between the calvaria and long bone revealed by three-dimensional fluorescence morphometry, possibly reflecting distinct mechano-adaptations and sensitivities
  Akiko Himeno-Ando; Yuichi Izumi; Akira Yamaguchi; Tadahiro Iimura
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 417, 2, 765, 770, Jan. 2012, [Peer-reviewed]
  English, Scientific journal
• Comparative morphology of the osteocyte lacunocanalicular system in various vertebrates
  Lei Cao; Takeshi Moriishi; Toshihiro Miyazaki; Tadahiro Iimura; Miwako Hamagaki; Ayako Nakane; Yoshihiro Tamamura; Toshihisa Komori; Akira Yamaguchi
  JOURNAL OF BONE AND MINERAL METABOLISM, 29, 6, 662, 670, Nov. 2011, [Peer-reviewed]
  English, Scientific journal
• Illumination of vertebrate development by fluorescence live imaging
  IIMURA Tadahiro; SUGIYAMA Mayu; MAKINO Yuji; NAKANE Ayako; WATANABE Takashi; YAMAGUCHI Akira
  Cytometry Research, 20, 3, 57, 63, Japan Cytometry Society, 25 Mar. 2011
  English,

  Development of organism is a dynamic but coordinated process that involves cell proliferation, migration and changes in cell function. Molecular biology and genome science promoted this realm of biomedical science by elucidating various common rules. Fluorescence live imaging has made it possible to quantitatively analyze multicellular process in 4 dimensions, thus providing coherent understandings of distinct levels of description form molecular levels to tissue, organ and organism. Application of this approach has given further insight and comprehension of dynamic process, not mere description of molecular hierarchy, into developmental biology and medicine. In this review paper, we, through introducing current topics in the body patterning, overview how the live imaging of fluorescent proteins has shed new lights on developmental biology.

• イヌの口腔粘膜線維芽細胞とrhBMP2を利用した骨再生療法の可能性の検討
  梅原 康佑; 五十嵐 順正; 飯村 忠浩; 山口 朗
  口腔病学会雑誌, 78, 1, 46, 46, 口腔病学会, Mar. 2011
  Japanese
• Lighting up skeletal biology by fluorescent imaging
  Tadahiro Iimura; Mayu Sugiyama; Takashi Watanabe; Ayako Nakane; Yuji Makino; Akira Yamaguchi
  Journal of Oral Biosciences, 53, 2, 97, 108, Japanese Association for Oral Biology, 2011, [Peer-reviewed]
  English
• Deficiency of Chemokine Receptor CCR1 Causes Osteopenia Due to Impaired Functions of Osteoclasts and Osteoblasts
  Akiyoshi Hoshino; Tadahiro Iimura; Satoshi Ueha; Sanshiro Hanada; Yutaka Maruoka; Mitsuori Mayahara; Keiko Suzuki; Toshio Imai; Masako Ito; Yoshinobu Manome; Masato Yasuhara; Takaaki Kirino; Akira Yamaguchi; Kouji Matsushima; Kenji Yamamoto
  JOURNAL OF BIOLOGICAL CHEMISTRY, 285, 37, 28826, 28837, Sep. 2010, [Peer-reviewed]
  English, Scientific journal
• Roles of Interleukin-6 and Parathyroid Hormone-Related Peptide in Osteoclast Formation Associated with Oral Cancers Significance of Interleukin-6 Synthesized by Stromal Cells in Response to Cancer Cells
  Kou Kayamori; Kei Sakamoto; Tomoki Nakashima; Hiroshi Takayanagi; Kei-ichi Morita; Ken Omura; Su Tien Nguyen; Yoshio Miki; Tadahiro Iimura; Akiko Himeno; Takumi Akashi; Hisafumi Yamada-Okabe; Etsuro Ogata; Akira Yamaguchi
  AMERICAN JOURNAL OF PATHOLOGY, 176, 2, 968, 980, Feb. 2010, [Peer-reviewed]
  English, Scientific journal
• Roles of interleukin-6 and parathyroid hormone-related peptide in osteoclast formation associated with oral cancers: Significance of interleukin-6 synthesized by stromal cells in response to cancer cells
  Kou Kayamori; Kei Sakamoto; Tomoki Nakashima; Hiroshi Takayanagi; Kei-Ichi Morita; Ken Omura; Su Tien Nguyen; Yoshio Miki; Tadahiro Iimura; Akiko Himeno; Takumi Akashi; Hisafumi Yamada-Okabe; Etsuro Ogata; Akira Yamaguchi
  American Journal of Pathology, 176, 2, 968, 980, Elsevier Inc., 2010, [Peer-reviewed]
  English, Scientific journal
• Hox genes, a molecular constraint for the development and evolution of the the Vertebrate Body Plan
  Tadahiro Iimura; Akiko Himeno; Ayako Nakane; Akira Yamaguchi
  Journal of Oral Biosciences, 52, 2, 155, 163, Japanese Association for Oral Biology, 2010, [Peer-reviewed]
  English
• Illuminating cell-cycle progression in the developing zebrafish embryo
  Mayu Sugiyama; Asako Sakaue-Sawano; Tadahiro Iimura; Kiyoko Fukami; Tetsuya Kitaguchi; Koichi Kawakami; Hitoshi Okamoto; Shin-ichi Higashijima; Atsushi Miyawaki
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 106, 49, 20812, 20817, Dec. 2009, [Peer-reviewed]
  English, Scientific journal
• ESTABLISHMENT OF HOX VERTEBRAL IDENTITIES IN THE EMBRYONIC SPINE PRECURSORS
  Tadahiro Iimura; Nicolas Denans; Olivier Pourquie
  HOX GENES, 88, 201, +, 2009, [Peer-reviewed]
  English, In book
• bHLH proteins and their role in somitogenesis
  Miguel Maroto; Tadahiro Iimura; J. Kim Dale; Yasumasa Bessho
  SOMITOGENESIS, 638, 124, 139, 2008, [Peer-reviewed]
  English, Scientific journal
• Manipulation and electroporation of the avian segmental plate and Somites in vitro
  Tadahiro Iimura; Olivier Pourquie
  AVIAN EMBRYOLOGY, 2ND EDITION, 87, 257, +, 2008, [Peer-reviewed]
  English, In book
• Hox genes in time and space during vertebrate body formation
  Tadahiro Iimura; Olivier Pourquie
  DEVELOPMENT GROWTH & DIFFERENTIATION, 49, 4, 265, 275, May 2007, [Peer-reviewed]
  English
• Dual mode of paraxial mesoderm formation during chick gastrulation
  Tadahiro Limura; Xuesong Yang; Cornelis J. Weijer; Olivier Pourquie
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 104, 8, 2744, 2749, Feb. 2007, [Peer-reviewed]
  English, Scientific journal
• Colinear Activation of Hox Genes Controls the Timing of Mesoderm Formation: A New Linkage between Time and Space during Vertebrate Body Formation
  飯村忠浩
  細胞工学, 25, 11, 1294, 1299, (株)学研メディカル秀潤社, Oct. 2006
  Japanese
• Collinear activation of Hoxb genes during gastrulation is linked to mesoderm cell ingression
  Tadahiro Iimura; Olivier Pourquie
  NATURE, 442, 7102, 568, 571, Aug. 2006, [Peer-reviewed]
  English, Scientific journal
• Development and proliferation: Segmentation clock.
  飯村忠浩
  生体の科学, 55, 5, 458, 459, (公財)金原一郎記念医学医療振興財団, Oct. 2004
  Japanese
• 21世紀の歯科医学・医療 歯科医療からみた再生医学 歯・歯周組織の再生医療 頭部神経堤細胞は,頭蓋顔面領域における胚性幹細胞である
  江藤 一洋; 飯村 忠浩
  日本歯科医学会誌, 22, 92, 95, 日本歯科医学会, Mar. 2003
  Japanese
• Phosphate depletion enhances bone morphogenetic protein-4 gene expression in a cultured mouse marrow stromal cell line ST2
  M Goseki-Sone; A Yamada; R Hamatani; L Mizoi; T Iimura; Ezawa, I
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 299, 3, 395, 399, Dec. 2002, [Peer-reviewed]
  English, Scientific journal
• Synergistic effect of fibroblast growth factor-4 in ectopic bone formation induced by bone morphogenetic protein-2
  K Kubota; S Iseki; S Kuroda; S Oida; T Iimura; WR Duarte; K Ohya; Ishikawa, I; S Kasugai
  BONE, 31, 4, 465, 471, Oct. 2002, [Peer-reviewed]
  English, Scientific journal
• 頭蓋顔面骨格の形の分化とパラドックス 頭部神経堤細胞は将来の骨の形を記憶しているのか?
  飯村 忠浩
  The Quintessence, 21, 10, 2219, 2219, クインテッセンス出版(株), Oct. 2002
  Japanese
• 時を刻む遺伝子と脊椎動物の発生 時間遺伝子と頭蓋顔面の進化
  飯村 忠浩
  The Quintessence, 21, 8, 1779, 1779, クインテッセンス出版(株), Aug. 2002
  Japanese
• The effect of swimming on cartilage formation
  A Yamada; Y Maruoka; K Asahi; T Iimura; S Oida; Ezawa, I; M Goseki-Sone
  JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, 48, 3, 238, 241, Jun. 2002, [Peer-reviewed]
  English, Scientific journal
• 時を刻む遺伝子と脊椎動物の発生 幹細胞と時間遺伝子の関係
  飯村 忠浩
  The Quintessence, 21, 6, 1368, 1368, クインテッセンス出版(株), Jun. 2002
  Japanese
• 時を刻む遺伝子と脊椎動物の発生 頭や顔の発生と時計遺伝子の関係
  飯村 忠浩
  The Quintessence, 21, 4, 907, 907, クインテッセンス出版(株), Apr. 2002
  Japanese
• Onset of the segmentation clock in the chick embryo: evidence for oscillations in the somite precursors in the primitive streak
  C Jouve; T Iimura; O Pourquie
  DEVELOPMENT, 129, 5, 1107, 1117, Mar. 2002, [Peer-reviewed]
  English, Scientific journal
• 時を刻む遺伝子と脊椎動物の発生 骨のかたちと発生における時間の関係
  飯村 忠浩
  The Quintessence, 21, 2, 451, 451, クインテッセンス出版(株), Feb. 2002
  Japanese
• 頭蓋顔面骨格の発生と線維芽細胞増殖因子(FGF) 頭蓋骨癒合症と骨芽細胞の分化メカニズム
  飯村 忠浩
  The Quintessence, 20, 12, 2603, 2603, クインテッセンス出版(株), Dec. 2001
  Japanese
• 頭蓋顔面骨格の発生と線維芽細胞増殖因子(FGF) FGF2は頭部神経堤細胞の骨格系細胞への分化を刺激する
  飯村 忠浩
  The Quintessence, 20, 10, 2142, 2142, クインテッセンス出版(株), Oct. 2001
  Japanese
• FGF-8とBMP-4は上下顎隆起の発生する場所を決めている
  飯村 忠浩
  The Quintessence, 20, 8, 1697, 1697, クインテッセンス出版(株), Aug. 2001
  Japanese
• Msx-1及びMax-2のダブルノックアウトマウスと歯の発生
  飯村 忠浩
  The Quintessence, 20, 6, 1243, 1243, クインテッセンス出版(株), Jun. 2001
  Japanese
• Msx-2はエナメル芽細胞の発生に必須の遺伝子である
  飯村 忠浩
  The Quintessence, 20, 4, 176, 176, クインテッセンス出版(株), Apr. 2001
  Japanese
• 【再生医学の最前線】 歯・顎顔面の発生と再生
  飯村 忠浩; 江藤 一洋
  分子心血管病, 2, 1, 41, 51, (株)先端医学社, Feb. 2001
  Japanese, 歯周病によって,歯周組織が失われると,歯そのものが健全でも歯を失うことになり,咀嚼や構音等の口腔機能を低下させる.しかしながら,歯周組織治療薬エムドゲインの登場によって,歯周組織を再生修復することが臨床的に可能になった.生物学的方法で口腔機能の改善が可能になったというこの事実は,歯科医療に大きなパラダイムの転換を迫るものになりそうである.エムドゲイン開発の大きなきっかけになったのは,発生の重要なイベントである組織間相互作用,即ち上皮-間葉の相互作用を模倣したことである.歯と顎顔面の発生学の立場から,歯科領域における再生医療の可能性について述べた
• 歯科医のためのDNA学再入門 メンデルのエンドウ豆から,ポストゲノムまで ヒトの遺伝子とポストゲノム時代の歯科医学
  飯村 忠浩
  The Quintessence, 20, 2, 403, 412, クインテッセンス出版(株), Feb. 2001
  Japanese
• Msx 1は口蓋裂の責任遺伝子か?
  飯村 忠浩
  The Quintessence, 20, 2, 421, 421, クインテッセンス出版(株), Feb. 2001
  Japanese
• 【21世紀の歯科医学 再生歯科医学の幕開け】 21世紀の分子歯科医学 幹細胞と再生歯科医学
  飯村 忠浩; 江藤 一洋
  The Quintessence, 20, 1, 85, 94, クインテッセンス出版(株), Jan. 2001
  Japanese
• メンデルのエンドウ豆から,ポストゲノムまで(その1) 遺伝子,染色体,DNA,そして誰でも持っている遺伝子異常
  飯村 忠浩
  The Quintessence, 20, 1, 207, 217, クインテッセンス出版(株), Jan. 2001
  Japanese
• 先天的な歯の数の減少と遺伝子変異 歯数の減少は進化か? あるいは遺伝子異常か?
  飯村 忠浩
  The Quintessence, 19, 12, 2531, 2531, クインテッセンス出版(株), Dec. 2000
  Japanese
• FRONT ATLAS 頭蓋顎顔面の分子発生学 遺伝子DNAは発生をどう説明するのか? 遺伝子・かたちの変化・そして時間
  飯村 忠浩; 江藤 一洋
  The Quintessence, 19, 12, 2353, 2367, クインテッセンス出版(株), Dec. 2000
  Japanese
• 頭蓋顎顔面の分子発生学 脳神経の発生 脳神経を解きほぐす
  飯村 忠浩; 江藤 一洋
  The Quintessence, 19, 11, 2147, 2154, クインテッセンス出版(株), Nov. 2000
  Japanese
• アトラス特別講座 頭蓋顎顔面の分子発生学 頭蓋の発生と発生異常
  飯村 忠浩; 江藤 一洋
  The Quintessence, 19, 10, 1933, 1939, クインテッセンス出版(株), Oct. 2000
  Japanese
• New Bio-science for Dental Field 過剰歯と鎖骨頭蓋異形成症の責任遺伝子(CBFA1) その変異がもたらすもの
  飯村 忠浩
  The Quintessence, 19, 10, 2123, 2123, クインテッセンス出版(株), Oct. 2000
  Japanese
• 頭蓋顎顔面の分子発生学 歯の発生 歯周組織の発生と再生
  飯村 忠浩; 江藤 一洋
  The Quintessence, 19, 9, 1723, 1729, クインテッセンス出版(株), Sep. 2000
  Japanese
• ラット初期胚におけるN-Deacetylase/N-Sulfotransferase(NDST)の発現
  池田 美子; 飯村 忠浩; 柳下 正樹
  歯科基礎医学会雑誌, 42, 5抄録集, 408, 408, (一社)歯科基礎医学会, Aug. 2000
  Japanese
• 頭蓋顎顔面の分子発生学 歯の発生 歯胚のシグナル中心 エナメル結節と歯冠形態の形成
  飯村 忠浩; 江藤 一洋
  The Quintessence, 19, 8, 1509, 1515, クインテッセンス出版(株), Aug. 2000
  Japanese
• 鎖骨頭蓋異形成症と代謝性骨疾患
  飯村 忠浩
  The Quintessence, 19, 8, 1700, 1700, クインテッセンス出版(株), Aug. 2000
  Japanese
• 頭蓋顎顔面の分子発生学 歯の発生 上皮-間葉相互作用と分子シグナルネットワーク
  飯村 忠浩; 江藤 一洋
  The Quintessence, 19, 7, 1315, 1322, クインテッセンス出版(株), Jul. 2000
  Japanese
• 若年性歯周炎とカテプシンC遺伝子の変異
  飯村 忠浩
  The Quintessence, 19, 6, 1291, 1291, クインテッセンス出版(株), Jun. 2000
  Japanese
• 頭蓋顎顔面の分子発生学 歯の発生 歯胚の誘導
  飯村 忠浩; 江藤 一洋
  The Quintessence, 19, 6, 1097, 1104, クインテッセンス出版(株), Jun. 2000
  Japanese
• 医学用語解説 Hox遺伝子群
  飯村 忠浩
  炎症と免疫, 8, 4, 468, 471, (株)先端医学社, Jun. 2000
  Japanese
• 頭蓋顎顔面の分子発生学 顎顔面の発生 頭部神経堤細胞は顎顔面の発生の主役である(2)
  飯村 忠浩; 江藤 一洋
  The Quintessence, 19, 5, 875, 882, クインテッセンス出版(株), May 2000
  Japanese
• 頭蓋顎顔面の分子発生学 顎顔面の発生 頭部神経堤細胞は顎顔面の発生の主役である(1)
  飯村 忠浩; 江藤 一洋
  The Quintessence, 19, 4, 661, 668, クインテッセンス出版(株), Apr. 2000
  Japanese
• 歯胚の発生位置を決めるメカニズム FGFとBMPの2つのシグナルが歯胚形成領域を決める
  飯村 忠浩
  The Quintessence, 19, 4, 847, 848, クインテッセンス出版(株), Apr. 2000
  Japanese
• 頭蓋顎顔面の分子発生学 顎顔面の発生 顔面の原基の形態形成
  飯村 忠浩; 江藤 一洋
  The Quintessence, 19, 3, 447, 453, クインテッセンス出版(株), Mar. 2000
  Japanese
• Molecular craniofacial embryology. Early Development, Gastrulation and Anterior Specification. Fertilized Egg to Specification of Anterior Structure.
  飯村忠浩; 江藤一洋
  Quintessence, 19, 2, 233, 240, クインテッセンス出版(株), Feb. 2000
  Japanese
• 胚発生における歯種の決定メカニズム 切歯か臼歯かは胚発生過程の顎のなかで決定される
  飯村 忠浩
  The Quintessence, 19, 2, 413, 414, クインテッセンス出版(株), Feb. 2000
  Japanese
• Introduction to Molecular Craniofacial Embryology. Facial Primordia: The Embryonic Origin of Facial Structures.
  飯村忠浩; 江藤一洋
  Quintessence, 19, 1, 3, 9, クインテッセンス出版(株), Jan. 2000
  Japanese
• Overexpression of nm23-H2/NDP kinase B in a human oral squamous cell carcinoma cell line results in reduced metastasis, differentiated phenotype in the metastatic site, and growth factor-independent proliferative activity in culture
  H Miyazaki; M Fukuda; Y Ishijima; Y Takagi; T Iimura; A Negishi; R Hirayama; N Ishikawa; T Amagasa; N Kimura
  CLINICAL CANCER RESEARCH, 5, 12, 4301, 4307, Dec. 1999
  English, Scientific journal
• Phosphate depletion enhances tissue-nonspecific alkaline phosphatase gene expression in a cultured mouse marrow stromal cell line ST2
  M Goseki-Sone; A Yamada; K Asahi; A Hirota; Ezawa, I; T Iimura
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 265, 1, 24, 28, Nov. 1999, [Peer-reviewed]
  English, Scientific journal
• Anabolic effect of aminoterminally truncated fibroblast growth factor 4 (FGF4) on bone
  S Kuroda; S Kasugai; S Oida; T Iimura; K Ohya; T Ohyama
  BONE, 25, 4, 431, 437, Oct. 1999, [Peer-reviewed]
  English, Scientific journal
• 分子発生 神経堤細胞の分布パターンと成長因子に対する応答性の関連性 顔面組織の分化・形態形成を理解する鍵
  飯村 忠浩; 今井 元; 青戸 一司; 江藤 一洋
  口腔病学会雑誌, 66, 3, 295, 295, 口腔病学会, Sep. 1999
  Japanese
• 歯根膜組織におけるS100A4カルシウム結合タンパクの発現とその生理的役割
  ワーグナー ドゥアルテ; 池田 かおり; 石川 烈; 春日井 昇平; 大谷 啓一; 飯村 忠浩; 大井田 新一郎
  口腔病学会雑誌, 66, 2, 223, 223, 口腔病学会, 30 Jun. 1999
  Japanese
• Molecular diagnosis of hypophosphatasia with severe periodontitis
  H Watanabe; M Goseki-Sone; T Iimura; S Oida; H Orimo; Ishikawa, I
  JOURNAL OF PERIODONTOLOGY, 70, 6, 688, 691, Jun. 1999, [Peer-reviewed]
  English, Scientific journal
• Periodontal ligament cells secrete S100A4 protein, an inhibitor of mineralization.
  WR Duarte; S Kasugai; T Iimura; H Kondo; S Oida; K Takenaga; K Ohya; Ishikawa, I
  JOURNAL OF DENTAL RESEARCH, 78, 5, 1124, 1124, May 1999, [Peer-reviewed]
  English
• Expression of mRNA encoding tissue-nonspecific alkaline phosphatase in human dental tissues
  M Goseki-Sone; T Iimura; K Takeda; A Nifuji; Y Ogata; M Yanagishita; S Oida
  CALCIFIED TISSUE INTERNATIONAL, 64, 2, 160, 162, Feb. 1999, [Peer-reviewed]
  English, Scientific journal
• Extracellular role of S100A4 calcium-binding protein in the periodontal ligament
  WR Duarte; T Iimura; K Takenaga; K Ohya; Ishikawa, I; S Kasugai
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 255, 2, 416, 420, Feb. 1999, [Peer-reviewed]
  English, Scientific journal
• Effects of mechanical stress on the mRNA expression of S100A4 and cytoskeletal components by periodontal ligament cells
  Wagner Rodrigues Duarte; Yuko Mikuni-Takagaki; Toshio Kawase; Tadahiro Iimura; Shinichiro Oida; Keiichi Ohya; Keizo Takenaga; Isao Ishikawa; Shohei Kasugai
  Journal of Medical and Dental Sciences, 46, 3, 117, 122, 1999, [Peer-reviewed]
  English, Scientific journal
• Expression of the mutant (1735T-DEL) tissue-nonspecific alkaline phosphatase gene from hypophosphatasia patients
  M Goseki-Sone; H Orimo; T Iimura; H Miyazaki; K Oda; H Shibata; M Yanagishita; Y Takagi; H Watanabe; T Shimada; S Oida
  JOURNAL OF BONE AND MINERAL RESEARCH, 13, 12, 1827, 1834, Dec. 1998, [Peer-reviewed]
  English, Scientific journal
• 哺乳類顔面軟骨誘導因子としてのBMPの役割
  飯村 忠浩; 今井 元; 石黒 聡子; 池田 美子; 江藤 一洋
  歯科基礎医学会雑誌, 40, 抄録, 393, 393, (一社)歯科基礎医学会, Sep. 1998
  Japanese
• Partial breakdown of glycated alkaline phosphatases mediated by reactive oxygen species
  Koyama, I; M Yakushijin; M Goseki; T Iimura; T Sato; M Sonoda; S Hokari; T Komoda
  CLINICA CHIMICA ACTA, 275, 1, 27, 41, Jul. 1998, [Peer-reviewed]
  English, Scientific journal
• Hypophosphatasia: Identification of five novel missense mutations (G507A, G705A, A748G, T1155C, G1320A) in the tissue-nonspecific alkaline phosphatase gene among Japanese patients
  M Goseki-Sone; H Orimo; T Iimura; Y Takagi; H Watanabe; K Taketa; S Sato; H Mayanagi; T Shimada; S Oida
  HUMAN MUTATION, 11, S263, S267, 1998, [Peer-reviewed]
  English, Scientific journal
• cDNA cloning of S100 calcium-binding proteins from bovine periodontal ligament and their expression in oral tissues
  W. R. Duarte; S. Kasugai; T. Iimura; S. Oida; K. Takenaga; K. Ohya; I. Ishikawa
  Journal of Dental Research, 77, 9, 1694, 1699, Intern. and American Associations for Dental Research, 1998, [Peer-reviewed]
  English, Scientific journal
• Gene analysis of 7 japanese families with hypophosphatasia
  Hideo Orimo; Masae Goseki-Sone; Tadahiro Iimura; Yuzo Takagi; Hisashi Watanabe; Kazuhisa Taketa; Seiji Sato; Hideaki Mayanagi; Akira Ohtake; Shinichiro Oida; Takashi Shimada
  Japanese Journal of Human Genetics, 42, 103, Dec. 1997, [Peer-reviewed]
  Hypophosphatasia (HOPS) is an autosomal recessive disease with skeretal hypomineralization due to deficiency of tissue-nonspecific alkaline phosphatase (TNSALP). We screened the TNSALP gene in 7 Japanese families with HOPS using PCR-SSCP-direct sequencing method and found 9 novel mutations. E281K, AF310, and frameshifts downstream from S328 and from L503 were found in 3 cases of perinatal/infantile type. F310L and V365I, A160T and F310L were found in a childhood type and an adult type, respectively. A94T, E174G and frameshift from L503 were found in 2 cases of odontohypophosphatasia. Partial intronic sequences of the TNSALP gene were determined to design the PCR primers that amplify whole coding exons. The mutations found in Japanese HOPS were completely different from those in North America and there were no clear relations between the mutation sites and phenotypes.
• Characterization of two length cDNA for human MSX-2 from dental pulp-derived cells
  T Iimura; K Takeda; M Goseki; M Maruoka; S Sasaki; S Oida
  DNA SEQUENCE, 8, 1-2, 87, 92, 1997, [Peer-reviewed]
  English, Scientific journal
• Pax-6 is involved in the specification of hindbrain motor neuron subtype
  Noriko Osumi; Arisa Hirota; Hideyo Ohuchi; Masato Nakafuku; Tadahiro Iimura; Shigeru Kuratani; Michio Fujiwara; Sumihare Noji; Kazuhiro Eto
  Development, 124, 15, 2961, 2972, 1997, [Peer-reviewed]
  English, Scientific journal
• Effects of ovariectomy on intestinal alkaline phosphatase expression in rats
  Hiroko N. Matsumoto; Asako Yamamoto; Tadahiro Iimura; Shinichiro Oida; Ikuko Ezawa; Satoshi Sasaki; Masae Goseki-Sone
  Journal of Nutritional Science and Vitaminology, 43, 5, 529, 539, Center for Academic Publications Japan, 1997, [Peer-reviewed]
  English, Scientific journal
• Characterization of two length cDNA for human MSX-2 from dental pulp-derived cells
  Tadahiro Iimura; Kohsuke Takeda; Masae Gosekp; Yutaka Maruoka; Satoshi Sasaki; Shinichro Oida
  Mitochondrial DNA, 8, 1-2, 87, 92, 1997, [Peer-reviewed]
  English, Scientific journal
• Expression of mRNA encoding intestinal type alkaline phosphatase in rat liver and its increase by fat-feeding
  M GosekiSone; S Oida; T Iimura; A Yamamoto; HN Matsumoto; N Omi; K Takeda; Y Maruoka; Ezawa, I; S Sasaki
  LIVER, 16, 6, 358, 364, Dec. 1996, [Peer-reviewed]
  English, Scientific journal
• Autocrine motility factor and its receptor expressions in human oral squamous cell carcinoma (SCC) cells
  Y Niinaka; SI Oida; A Ishisaki; K Takeda; T Iimura; Y Maruoka; F Momose; A Negishi; H Ichijo; T Amagasa; S Sasaki; H Watanabe; A Raz
  INTERNATIONAL JOURNAL OF ONCOLOGY, 9, 3, 433, 438, Sep. 1996, [Peer-reviewed]
  English, Scientific journal
• Molecular structure of the mouse amelogenin genomic DNA
  S Oida; H Miyazaki; T Iimura; M Suzuki; S Sasaki; H Shimokawa
  DNA SEQUENCE, 6, 5, 307, 310, 1996
  English, Scientific journal
• Homeobox gene expression during bone formation induced by BMP
  T IImura; O Baba; Y Maruoka; K Takeda; S Sasaki; H Shimokawa; S Oida
  MOLECULAR AND DEVELOPMENTAL BIOLOGY OF CARTILAGE, 785, 274, 277, 1996, [Peer-reviewed]
  English, Scientific journal
• Molecular structure of the mouse amelogenin genomic DNA
  Shinichiro Oida; Hidetaka Miyazaki; Tadahiro Iimura; Michiko Suzuki; Satoshi Sasaki; Hitoyata Shimokawa
  Mitochondrial DNA, 6, 5, 307, 310, 1996, [Peer-reviewed]
  English, Scientific journal
• Molecular cloning of mouse amelogenin genes from mandibular cDNA library
  Shinichiro OIDA; Tadahiro IIMURA; Naoya ARAI; Kohsuke TAKEDA; Akira ISHISAKI; Yutaka MARUOKA; Tatuo TERASHIMA; Hitoyata SHIMOKAWA; Satoshi SASAKI; Deaprtment of Bilchemistry Faculty of Dentistry Tokyo Medical and Dental University; Deaprtment of Bilchemistry Faculty of Dentistry Tokyo Medical and Dental University; Department of First Oral and Maxillofacial Surgery Faculty of Dentistry Tokyo Medical and Dental University; Department of First Oral and Maxillofacial Surgery Faculty of Dentistry Tokyo Medical and Dental University; Department of First Oral and Maxillofacial Surgery Faculty of Dentistry Tokyo Medical and Dental University; Department of Second Oral and Maxillofacial Surgery Faculty of Dentistry Tokyo Medical and Dental University; Department of Anatomy Faculty of Dentistry Tokyo Medical and Dental University; Deaprtment of Bilchemistry Faculty of Dentistry Tokyo Medical and Dental University; Deaprtment of Bilchemistry Faculty of Dentistry Tokyo Medical and Dental University
  Journal of hard tissue biology = Journal of hard tissue biology, 42, 2, 64, 69, 1995
  English, Amelogenin is an enamel-specuflc protein expressed by the ameloblasts and a marker for the differentiation of the enamel epithelial cells. In this paper, we isolated two different cDNAs of amelogenin, which were spliced alternatively, from a mouse mandibular cDNA library using a PCRcloned mouse amelogenin cDNA as a probe. Nucleotide sequence analysis showed that both of them contained the non-coding first exon in comparison with the sequences of previously reported human and bovine amelogenin genomic DNA and RT-PCR cloned mouse amelogenin cDNA. Expression of amelogenin gene was investigated by in situ hybridization and the northern blot analysis using the cloned cDNAs of mouse amelogenin.
• CLONING AND SEQUENCE OF BONE MORPHOGENETIC PROTEIN-4 (BMP-4) FROM A HUMAN PLACENTAL CDNA LIBRARY
  S OIDA; T IIMURA; Y MARUOKA; K TAKEDA; S SASAKI
  DNA SEQUENCE, 5, 5, 273, 275, 1995
  English
• MOLECULAR-CLONING AND SEQUENCE OF BOVINE MSX-1 HOMEOBOX-CONTAINING GENE CDNA FROM A BOVINE ODONTOBLAST LIBRARY
  T IIMURA; S OIDA; K TAKEDA; Y MARUOKA; H SHIMOKAWA; K IBARAKI; S SASAKI
  DNA SEQUENCE, 5, 4, 233, 237, 1995
  English
• Molecular cloning and sequence of bovine msx-1 homeobox-containing gene cDNA from a bovine odontoblast library
  Tadahiro Iimura; Shinichiro Oida; Kohsuke Takeda; Yutaka Maruoka; Hitoyata Shimokawa; Kyomi Ibaraki; Satoshi Sasaki
  Mitochondrial DNA, 5, 4, 233, 237, Informa Healthcare, 1995, [Peer-reviewed]
  English, Scientific journal
• IDENTIFICATION OF BONE-TYPE ALKALINE-PHOSPHATASE MESSENGER-RNA FROM HUMAN PERIODONTAL-LIGAMENT CELLS
  M GOSEKI; S OIDA; K TAKEDA; Y OGATA; T IIMURA; Y MARUOKA; S SASAKI
  JOURNAL OF DENTAL RESEARCH, 74, 1, 319, 322, Jan. 1995, [Peer-reviewed]
  English, Scientific journal
• Expression of prostaglandin E receptor subtypes in bone: Expression of EP2 in bone development
  S. Kasugai; S. Oida; T. Iimura; N. Arai; K. Takeda; K. Ohya; S. Sasaki
  Bone, 17, 1, 1, 4, 1995, [Peer-reviewed]
  English, Scientific journal
• Cloning and sequence of bone morphogenetic protein 4 (BMP-4) from a human placental cDNA library
  Shinichiro Oida; Tadahiro Iimura; Yutaka Maruoka; Kohsuke Takeda; Satoshi Sasaki
  Mitochondrial DNA, 5, 5, 273, 275, Informa Healthcare, 1995, [Peer-reviewed]
  English, Scientific journal
• Production of functional human bone morphogenetic protein-2 using a baculovirus/Sf-9 insect cell system
  Y. Maruoka; S. Oida; T. Iimura; K. Takeda; I. Asahina; S. Enomoto; S. Sasaki
  Biochemistry and Molecular Biology International, 35, 5, 957, 963, 1995, [Peer-reviewed]
  English, Scientific journal
• Molecular cloning and expression of homeobox-containing genes during hard tissue development
  T. Iimura
  Kokubyo Gakkai zasshi. The Journal of the Stomatological Society, Japan, 61, 4, 590, 604, 口腔病学会, 01 Dec. 1994
  Japanese
• MOLECULAR-CLONING OF RAT BONE MORPHOGENETIC PROTEIN (BMP) TYPE IA RECEPTOR AND ITS EXPRESSION DURING ECTOPIC BONE-FORMATION INDUCED BY BMP
  K TAKEDA; S OIDA; H ICHIJO; T IIMURA; Y MARUOKA; T AMAGASA; S SASAKI
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 204, 1, 203, 209, Oct. 1994, [Peer-reviewed]
  English, Scientific journal
• MODULATION OF RESPONSES TO TGF-BETA BY 1,25-DIHYDROXYVITAMIN-D-3 IN MG-63 OSTEOBLASTIC CELLS - POSSIBLE INVOLVEMENT OF REGULATION OF TGF-BETA TYPE-II RECEPTOR
  T IIMURA; S OIDA; H ICHIJO; M GOSEKI; Y MARUOKA; K TAKEDA; S SASAKI
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 204, 2, 918, 923, Oct. 1994, [Peer-reviewed]
  English, Scientific journal
• CHANGES IN HOMEOBOX-CONTAINING GENE-EXPRESSION DURING ECTOPIC BONE-FORMATION INDUCED BY BONE MORPHOGENETIC PROTEIN
  T IIMURA; S OIDA; K TAKEDA; Y MARUOKA; S SASAKI
  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 201, 2, 980, 987, Jun. 1994, [Peer-reviewed]
  English, Scientific journal
• Expression of bone morphogenetic protein genes in the human dental pulp cells
  K. Takeda; S. Oida; M. Goseki; T. Iimura; Y. Maruoka; T. Amagasa; S. Sasaki
  Bone, 15, 5, 467, 470, 1994, [Peer-reviewed]
  English, Scientific journal
• PCR amplification and cloning of a bone morphogenetic protein(BMP) c DNA
  S.Oida; A.Nifuji; M.Tamura; Y.Maruoka; T.Iimura; S.Sasaki
  Jpn. J. Oral Biol., 34, 5, 612, 615, 1992
• Fluorescent imaging approach and perspective for the circadian development and metabolism of bone
  Nakane A; Numano R; Takagi Y; Yamaguchi A; Iimura T
  Jounal of Bone Morphometry., 23, 41, 50, [Peer-reviewed]
  English

• 骨モデリング期におけるコラーゲン線維構築と骨細胞ネットワーク形成の関係について
  上岡寛; 橋本真奈; 高橋春香; 田畑香織; 長岡紀幸; 原徹; 飯村忠浩, 日本骨形態計測学会雑誌, 35, 1, 2025
• 多様な顕微鏡を用いた,骨細胞ネットワーク形成におけるコラーゲン細線維集束の重要性の解明
  橋本 真奈; 高橋 春香; 田畑 香織; 長岡 紀幸; 原 徹; 飯村 忠浩; 上岡 寛, 日本骨形態計測学会雑誌, 34, 2, 68, 69, Nov. 2024
  日本骨形態計測学会, Japanese
• AI駆動型形態計測を用いた頭蓋骨・下顎骨における骨形成促進薬テリパラチドの薬理作用特異性
  中西 徳子[木村]; 高倉 綾; 星 麻里絵[沼端]; 佐藤 嘉晃; 高尾 亮子; 飯村 忠浩, 日本骨形態計測学会雑誌, 34, 2, 80, 81, Nov. 2024
  日本骨形態計測学会, Japanese
• 骨粗鬆症モデルラットへの骨形成促進薬PTH製剤投与による下顎骨の骨小腔周囲性骨リモデリング調節
  中西 徳子; 星 麻里絵; 佐藤 嘉晃; 飯村 忠浩, 日本矯正歯科学会大会プログラム・抄録集, 83回, 186, 186, Oct. 2024
  (公社)日本矯正歯科学会, Japanese
• 顎骨の特殊性 骨代謝と薬理作用からみた顎骨の特殊性
  飯村 忠浩, 日本口腔科学会雑誌, 73, 2, 84, 84, Sep. 2024
  (NPO)日本口腔科学会, Japanese
• PTH1型受容体作用薬の薬理作用アップデート シグナル,骨微細構造,疼痛軽減
  飯村 忠浩, 日本整形外科学会雑誌, 98, 8, S1797, S1797, Sep. 2024
  (公社)日本整形外科学会, Japanese
• 骨粗鬆症モデルラットへのテリパラチド投与による下顎骨骨小腔の3次元形態変化 骨細胞性・骨小腔周囲リモデリング調節の可能性
  中西 徳子[木村]; 高倉 綾; 星 麻里絵[沼端]; 高尾 亮子; 飯村 忠浩, 日本骨代謝学会学術集会プログラム抄録集, 42回, 184, 184, Jun. 2024
  (一社)日本骨代謝学会, Japanese
• テリパラチドによるイヌ皮質骨代謝への薬理作用解明を目的とした微細構造解析 地理情報システム(GIS)とAI駆動型形態認識システムの応用
  星 麻里絵[(沼端]; 高倉 綾; 中西 徳子[木村]; 高尾 亮子; 飯村 忠浩, 日本骨代謝学会学術集会プログラム抄録集, 42回, 188, 188, Jun. 2024
  (一社)日本骨代謝学会, Japanese
• PTH1型受容体作動薬の薬理作用アップデート 細胞内シグナル、骨微細構造、疼痛軽減
  飯村 忠浩, 日本骨形態計測学会雑誌, 34, 1, 99, 99, May 2024
  日本骨形態計測学会, Japanese
• My Three-Sword style as a parent, a clinician and a researcher; Potential applications of an osteoporosis drug to orthodontics
  星(沼端)麻里絵; 星(沼端)麻里絵; 中西(木村)徳子; 中西(木村)徳子; 渡辺陽久; 渡辺陽久; 西浦まい; 西浦まい; 上野楓実; 上野楓実; 西本慎之介; 西本慎之介; 孝口里侑; 孝口里侑; 佐藤嘉晃; 飯村忠浩, 北海道矯正歯科学会雑誌, 52, 1, 2024
• 多様な顕微鏡を用いた,骨細胞ネットワーク形成におけるコラーゲン細線維束化の重要性の解明
  橋本真奈; 高橋春香; 田畑香織; 長岡紀幸; 原徹; 飯村忠浩; 上岡寛, 日本骨形態計測学会雑誌, 34, 1, 143, 143, 2024
  日本骨形態計測学会, Japanese
• 空間マッピング法とAI駆動型形態計測法による骨形成促進薬PTH製剤のイヌ皮質骨代謝への薬理作用解明
  星 麻里絵[沼端]; 中西 徳子[木村]; 高田 健太郎; 佐藤 嘉晃; 飯村 忠浩, 日本矯正歯科学会大会プログラム・抄録集, 82回, 178, 178, Nov. 2023
  (公社)日本矯正歯科学会, Japanese
• 骨粗鬆症モデルラットへの骨形成促進薬PTH製剤投与による下顎骨の骨細胞性リモデリング調節
  中西 徳子[木村]; 星 麻里絵[沼端]; 佐藤 嘉晃; 飯村 忠浩, 日本矯正歯科学会大会プログラム・抄録集, 82回, 180, 180, Nov. 2023
  (公社)日本矯正歯科学会, Japanese
• 「臨床応用を目指した骨・軟骨研究」 骨代謝改善薬のドラッグリポジショニング研究
  飯村 忠浩, Journal of Oral Biosciences Supplement, 2023, [MS3, 02], Sep. 2023
  (一社)歯科基礎医学会, Japanese
• PTH製剤の新規薬理作用 骨コラーゲン配列,皮質骨リモデリング,疼痛軽減
  飯村 忠浩, 日本整形外科学会雑誌, 97, 8, S1813, S1813, Aug. 2023
  (公社)日本整形外科学会, Japanese
• Skeletal-Vascular Interactions in Bone Development, Homeostasis, and Pathological Destruction
  Haruhisa Watanabe; Nako Maishi; Marie Hoshi-Numahata; Mai Nishiura; Atsuko Nakanishi-Kimura; Kyoko Hida; Tadahiro Iimura, International Journal of Molecular Sciences, 24, 13, 10912, 10912, 30 Jun. 2023, [Peer-reviewed]
  Bone is a highly vascularized organ that not only plays multiple roles in supporting the body and organs but also endows the microstructure, enabling distinct cell lineages to reciprocally interact. Recent studies have uncovered relevant roles of the bone vasculature in bone patterning, morphogenesis, homeostasis, and pathological bone destruction, including osteoporosis and tumor metastasis. This review provides an overview of current topics in the interactive molecular events between endothelial cells and bone cells during bone ontogeny and discusses the future direction of this research area to find novel ways to treat bone diseases., MDPI AG, English, Introduction scientific journal
• AI駆動型形態計測を用いた頭蓋骨・下顎骨における骨形成促進薬テリパラチドの薬理作用特異性
  中西 徳子[木村]; 高倉 綾; 星 麻里絵[沼端]; 佐藤 嘉晃; 高尾 亮子; 飯村 忠浩, 日本骨形態計測学会雑誌, 33, 1, 171, 171, May 2023
  日本骨形態計測学会, Japanese
• 破骨細胞の分化成熟におけるPyk2の役割
  西浦 まい; 八若 保孝; 飯村 忠浩; 李 智媛, 日本骨形態計測学会雑誌, 33, 1, 197, 197, May 2023
  日本骨形態計測学会, Japanese
• 薬剤関連顎骨壊死・臨床研究のフロンティア 骨形成促進薬の薬理作用と顎骨壊死への影響
  庵 敬; 北川 善政; 飯村 忠浩, 日本骨形態計測学会雑誌, 33, 1, 104, 104, May 2023
  日本骨形態計測学会, Japanese
• 薬剤関連顎骨壊死・臨床研究のフロンティア MRONJの薬理病態と治療
  渡辺 陽久; 北川 善政; 飯村 忠浩, 日本骨形態計測学会雑誌, 33, 1, 105, 105, May 2023
  日本骨形態計測学会, Japanese
• 女性における顎関節の退行性病変の病態と血清CCL5値の上昇との関連性
  渡辺 陽久; 庵 敬; 李 智媛; 梶井 貴史; 高倉 綾; 高尾 亮子; 北川 善政; 丸岡 豊; 飯村 忠浩, 日本骨形態計測学会雑誌, 33, 1, 150, 150, May 2023
  日本骨形態計測学会, Japanese
• 【最新の骨粗鬆症学(第2版)-骨粗鬆症学の最新知見-】骨研究フロンティア 骨代謝を調節する微細環境 骨代謝を調節する微小環境 血管系,神経系
  飯村 忠浩, 日本臨床, 81, 増刊1 最新の骨粗鬆症学, 118, 122, Jan. 2023
  (株)日本臨床社, Japanese
• AI駆動型形態計測法とGIS空間マッピング法による骨粗鬆症治療薬の新規薬理効果解析法の確立と薬理作用解明
  星(沼端)麻里絵; 星(沼端)麻里絵; 高倉綾; 中西(木村)徳子; 中西(木村)徳子; 渡辺陽久; 渡辺陽久; 高田健太郎; 西浦まい; 西浦まい; 佐藤嘉晃; 高尾(川端)亮子; 飯村忠浩, 日本顕微鏡学会北海道支部学術講演会講演要旨集, 2023 (CD-ROM), 2023
• 骨粗鬆症モデルラットにおける骨形成促進薬PTH製剤の頭蓋骨・下顎骨への薬理作用特異性
  中西 徳子; 佐藤 孝紀; 星 麻里絵; 佐藤 嘉晃; 飯村 忠浩, 日本矯正歯科学会大会プログラム・抄録集, 81回, 166, 166, Oct. 2022
  (公社)日本矯正歯科学会, Japanese
• RAW細胞の継代数が破骨細胞分化誘導系に与える影響
  柳澤 瞳子; 吉村 善隆; 早川 貴子; 福島 久夢; 飯村 忠浩; 佐藤 嘉晃, 北海道歯学雑誌, 43, 29, 35, Sep. 2022
  北海道歯学会, Japanese
• 最新の歯学 骨格痛における感覚神経節マクロファージの病態機能と創薬展開
  庵 敬; 佐藤 孝紀; 李 智媛; 北川 善政; 飯村 忠浩, 北海道歯学雑誌, 43, 2, 4, Sep. 2022
  骨・関節の障害に由来する慢性疼痛は骨格痛と呼ばれ、骨粗鬆症、変形性関節症、転移性骨腫瘍など多様な疾患群によって引き起こされる。脊髄後根神経節(DRG)は骨格系の一次求心性の神経細胞体が集合する感覚神経節である。近年、骨格痛の発症・遷延にはDRGにおけるマクロファージの活性化やそれに付随して生じる神経炎症が関わっていることが明らかになった。炎症性マクロファージ抑制や神経炎症をターゲットとした新規治療薬の創薬について述べた。, 北海道歯学会, Japanese
• PTHの薬理作用アップデート シグナル、骨コラーゲン配列への影響、投与頻度別効果、疼痛改善効果
  飯村 忠浩, 日本骨代謝学会学術集会プログラム抄録集, 40回, 109, 109, Jul. 2022
  (一社)日本骨代謝学会, Japanese
• 骨形態計測からわかった骨形成促進薬の動的作用～この結果をどう臨床に生かすか～ 骨形態計測と蛍光イメージング形態計測解析で見る、骨形成促進薬テリパラチドの骨微細構造への薬理作用
  飯村 忠浩, 日本骨形態計測学会雑誌, 32, 1, S84, S84, May 2022
  日本骨形態計測学会, Japanese
• AI-駆動型蛍光形態計測によるPTH製剤の骨微細構造および骨コラーゲン整調効果の解明
  佐藤 孝紀; 高倉 綾; 李 智媛; 高尾 亮子; 飯村 忠浩, 日本骨形態計測学会雑誌, 32, 1, S168, S168, May 2022
  日本骨形態計測学会, Japanese
• 3次元蛍光イメージングによる脳のミクログリア貪食能の定量評価法の確立
  渡辺陽久; 渡辺陽久; 李智媛; 北川善政; 飯村忠浩, 日本顕微鏡学会北海道支部学術講演会講演要旨集, 2022 (CD-ROM), 2022
• 3次元蛍光イメージングによる骨粗鬆症誘導性神経炎症の解析
  庵敬; 庵敬; 渡辺陽久; 渡辺陽久; 李智媛; 北川善政; 飯村忠浩, 日本顕微鏡学会北海道支部学術講演会講演要旨集, 2022 (CD-ROM), 2022
• SARS-CoV-2感染マウスを用いた肺血管内皮細胞の重症化関連遺伝子の解析
  武田遼; 武田遼; 伊藤航; 手代木孝仁; 佐々木道仁; 大場靖子; 大場靖子; 間石奈湖; 松田彩; 佐藤彰彦; 鳥羽晋輔; 李智媛; 飯村忠浩; 樋田泰浩; 北川善政; 澤洋文; 澤洋文; 澤洋文; 樋田京子, 日本血管生物医学会学術集会プログラム・抄録集, 30th (CD-ROM), 2022
• 骨粗鬆症治療薬PTH製剤による骨微細構造および骨コラーゲンの整調効果の解明 新規蛍光イメージング解析による定量的トポロジー解析による骨質評価法の確立(Teriparatide administration harmonizes the arrangement of bone collagen and bone microstructure: a novel fluorescence analysis to evaluate bone quality)
  佐藤 孝紀; 李 智媛; 飯村 忠浩; 高倉 綾; 高尾 亮子; 徳永 和明; 松森 はるか, Journal of Oral Biosciences Supplement, 2021, 154, 154, Oct. 2021
  (一社)歯科基礎医学会, Japanese
• 薬理作用からみた薬剤関連顎骨壊死
  飯村忠浩; 高倉綾, 日本骨形態計測学会雑誌, 31, 2, S85, S85, 2021
  日本骨形態計測学会, Japanese
• 新規蛍光形態計測法および第2次高調波イメージングによる骨粗鬆症治療薬PTH製剤による骨質改善効果の定量評価法の確立
  佐藤孝紀; 李智媛; 飯村忠浩, 日本顕微鏡学会北海道支部学術講演会講演要旨集, 2021 (CD-ROM), 2021
• Teriparatide administration harmonizes the arrangement of bone collagen and bone microstructure-a novel fluorescence analysis to evaluate bone quality-
  佐藤孝紀; LEE JW; 飯村忠浩, Journal of Oral Biosciences Supplement (Web), 2021, 2021
• 歯科薬理学の新しい潮流 骨代謝疾患とドラッグリポジショニング研究
  飯村 忠浩, Journal of Oral Biosciences Supplement, 2020, 76, 76, Sep. 2020
  (一社)歯科基礎医学会, Japanese
• 霊長類を用いた歯髄再生制御機構の定量的蛍光イメージング解析
  戸井田 侑; 佐藤 孝紀; 飯村 忠浩, Journal of Oral Biosciences Supplement, 2020, 349, 349, Sep. 2020
  (一社)歯科基礎医学会, Japanese
• PTH製剤は骨コラーゲン線維の連続性を高める 非線形光学を応用した霊長類椎体骨コラーゲンの定量的トポロジー解析
  佐藤 孝紀; 戸井田 侑; 飯村 忠浩, Journal of Oral Biosciences Supplement, 2020, 350, 350, Sep. 2020
  (一社)歯科基礎医学会, Japanese
• Send our paradigm shifts to the world-Drug discovery and development for stomatology and medical sciences
  飯村忠浩, 北海道歯学雑誌, 40, 2, 127, 127, 2020
  北海道歯学会, Japanese
• ラット骨粗鬆症性痛覚過敏に対するテリパラチドの薬理作用の解明-蛍光形態計測と分子オミクスによる解析-
  田中智哉; 田中智哉; 高倉綾; 高倉綾; 高尾亮子; 李智媛; 飯村忠浩, 日本骨形態計測学会雑誌, 30, 1, S112, S112, 2020
  日本骨形態計測学会, Japanese
• Neuropharmacological effect of PTH as a skeletal pain reliever
  戸井田侑; 佐藤孝紀; 山下航司; 山下航司; 劉雲青; 劉雲青; 田中智哉; 田中智哉; 飯村忠浩, 北海道歯学雑誌, 41, 1, 25, 26, 2020
  北海道歯学会, Japanese
• Sudachitin suppresses osteoclastogenesis
  萇崎誠治; 吉村善隆; 飯村忠浩; 山崎裕, 北海道歯学雑誌, 40, 2, 95, 104, 2020
  北海道歯学会, Japanese
• Linkage between HIV infection and bone resorptive diseases revealed by a novel pharmacology on osteoimmunology
  佐藤孝紀; 戸井田侑; 服部倫寛; 服部倫寛; LI J.; 飯村忠浩, 北海道歯学雑誌, 41, 1, 27, 29, 2020
  北海道歯学会, Japanese
• Edaravone suppresses osteoclastogenesis
  大西香織; 吉村善隆; 飯村忠浩; 山崎裕, 北海道歯学雑誌, 40, 2, 105, 113, 2020
  北海道歯学会, Japanese
• Metabolic bone diseases and drug repositioning researches
  飯村忠浩, Journal of Oral Biosciences Supplement (Web), 2020, 2020
• Quantitative fluorescence imaging analysis of tooth pulp regeneration in primate model
  戸井田侑; 佐藤孝紀; 飯村忠浩, Journal of Oral Biosciences Supplement (Web), 2020, 2020
• Teriparatide administration augments the linearity of bone collagen fibers-quantitative collagen topology analyses by an advanced nonlinear optics imaging-
  佐藤孝紀; 戸井田侑; 飯村忠浩, Journal of Oral Biosciences Supplement (Web), 2020, 2020
• Mechanical Loading-induced Changes in the Sclerostin Distribution around Osteocytes
  Ryuta Osumi; Yoshihito Ishihara; Naoya Odagaki; Ziyi Wang; Hiroshi Kamioka; Tadahiro Iimura, Journal of Bone and Mineral Research, 34, S1, S153, S153, Sep. 2019, [International Magazine]
  Poster Presentation on 2019 ASBMR Annual Meeting.
  Available at https://www.asbmr.org/meetings/annualmeeting/AbstractDetail?aid=65f25eb6-4dd7-42de-adaf-04ba34206836
  
  Poster Sessions, Presentation Number: SAT-562
  Session: Poster Session I
  Saturday, September 21, 2019 12:30 PM - 2:30 PM, Orange County Convention Center , West Hall C, The American Society for Bone and Mineral Research (ASBMR), English, Summary international conference
• Sclerostinの機械的負荷に伴う分布域変化の新たな機構の解析
  大住隆太; 王紫儀; 石原嘉人; 小田垣直弥; 飯村忠浩; 上岡寛, 日本骨形態計測学会雑誌, 29, 1, S128, S128, 2019
  日本骨形態計測学会, Japanese
• マルチモード蛍光観察による新規の骨代謝病態機能探索
  飯村忠浩; 李智媛, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 123rd, 2018
• 骨組織・細胞の形態学的観察法-光学顕微鏡の基礎から論文映えする写真の撮り方-
  飯村忠浩; 飯村忠浩; 飯村忠浩, 日本骨代謝学会学術集会プログラム抄録集, 36th, 127, 127, 2018
  (一社)日本骨代謝学会, Japanese
• 新規デンスグラニュールタンパク質PV1のマラリア原虫感染赤血球内タンパク質輸送における機能
  森田将之; 高島英造; 長岡ひかる; NTEGE Edward H.; KANOI Bernard N.; 伊藤大輔; LEE Ji-Won; 徳永和明; 飯村忠浩; 鳥居本美; 坪井敬文, 日本寄生虫学会大会プログラム・抄録集, 87th, 2018
• Zscan10はHaptoglobinの転写を介して破骨細胞分化を負に制御する
  柳原裕太; 柳原裕太; 井上和樹; 井上和樹; 佐伯法学; 佐伯法学; 沢田雄一郎; 沢田雄一郎; LEE Jiwon; 飯村忠浩; 飯村忠浩; 今井祐記; 今井祐記, 日本骨代謝学会学術集会プログラム抄録集, 36th, 157, 157, 2018
  (一社)日本骨代謝学会, Japanese
• 機械的負荷による骨細胞Sclerostin発現の分布域変化の解析
  大住隆太; WANG Z.; 石原嘉人; 小田垣直弥; 飯村忠浩; 上岡寛, 日本矯正歯科学会大会プログラム・抄録集, 77th, 245, 245, 2018
  (公社)日本矯正歯科学会, Japanese
• 卵巣摘除ラットの痛覚過敏に対するテリパラチドの改善作用および神経特異的バイオシグナルの同定
  田中智哉; 田中智哉; 高尾亮子; 飯村忠浩; 飯村忠浩; 飯村忠浩; 飯村忠浩, 日本骨代謝学会学術集会プログラム抄録集, 36th, 158, 158, 2018
  (一社)日本骨代謝学会, Japanese
• FIB-SEMで探る骨形成
  上岡寛; 橋本真奈; 長岡紀幸; 飯村忠浩; 原徹, 日本分子生物学会年会プログラム・要旨集(Web), 41st, 2018
• デコンボリューション蛍光顕微鏡と超解像レーザー顕微鏡 骨の生物医学的マルチモード解析
  飯村 忠浩, Journal of Oral Biosciences Supplement, 2017, 108, 108, Sep. 2017
  (一社)歯科基礎医学会, Japanese
• 歯科インプラント埋入後に新生されたオステオンの構造特性
  是澤 和人; 松永 智; 小高 研人; 森田 純晴; 廣内 英智; 飯村 忠浩; 阿部 伸一, Journal of Oral Biosciences Supplement, 2017, 199, 199, Sep. 2017
  (一社)歯科基礎医学会, Japanese
• Unveiling a rhythmic regulatory mode hidden in developmental tissue growth by fluorescence live imaging-based mathematical modeling
  Tadahiro Iimura; Ji-Won Lee, Journal of Oral Biosciences, 59, 1, 6, 11, 01 Feb. 2017
  Japanese Association for Oral Biology, English, Book review
• 産学および分野横断連携研究の取り組みと成果
  飯村忠浩; 飯村忠浩; 飯村忠浩, 日本口腔科学会学術集会プログラム・抄録集, 71st, 2, 70, 70, 2017
  (NPO)日本口腔科学会, Japanese
• 骨形態計測法で明らかになったHIV治療薬標的分子CCR5の破骨細胞機能分化における必須の機能
  李智媛; 飯村忠浩; 飯村忠浩; 飯村忠浩; 飯村忠浩, 日本骨形態計測学会雑誌, 27, 1, S113, S113, 2017
  日本骨形態計測学会, Japanese
• 先進光学顕微鏡の骨形態計測法への応用展開
  飯村忠浩; 飯村忠浩; 飯村忠浩; 飯村忠浩, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 122nd, 2017
• テリパラチドによる皮質骨空隙形成は投与頻度に依存する~卵巣摘除ラットのマルチスケール骨形態解析~
  高倉綾; 高倉綾; 李智媛; 高尾亮子; 飯村忠浩; 飯村忠浩; 飯村忠浩; 飯村忠浩, 日本骨形態計測学会雑誌, 27, 1, S151, S151, 2017
  日本骨形態計測学会, Japanese
• デコンボリューション蛍光顕微鏡と超解像レーザー顕微鏡-骨の生物医学的マルチモード解析-
  飯村忠浩; 飯村忠浩, Journal of Oral Biosciences Supplement (Web), 2017, 2017
• マウスジェネティクスを用いたHIV感染患者の骨病態解明
  飯村忠浩; 飯村忠浩; 飯村忠浩; LEE J-W, Journal of Oral Biosciences Supplement (Web), 2017, 2017
• 卵巣摘除ラットの痛覚過敏およびテリパラチド作用についての機序検討
  田中智哉; 田中智哉; 高尾亮子; 伊藤彰敏; 飯村忠浩; 飯村忠浩, 日本骨粗鬆症学会雑誌, 3, Suppl.1, 259, 259, 2017
  (一社)日本骨粗鬆症学会, Japanese
• 高頻度のテリパラチド投与は皮質骨空隙形成を誘発する。-卵巣摘除ラットでの検討-
  高倉綾; 高倉綾; LEE Ji-Won; 高尾亮子; 飯村忠浩; 飯村忠浩; 飯村忠浩; 飯村忠浩, 日本骨代謝学会学術集会プログラム抄録集, 35th, 192, 192, 2017
  (一社)日本骨代謝学会, Japanese
• ヒト歯科インプラント周囲顎骨における構造特性
  松永智; 是澤和人; 小高研人; 松永智; 是澤和人; 小高研人; 吉成正雄; 飯村忠浩; 山口朗; 中野貴由; 阿部伸一, 日本骨形態計測学会雑誌, 27, 1, S135, S135, 2017
  日本骨形態計測学会, Japanese
• 赤血球内タンパク質輸送に関わる新規デンスグラニュールタンパク質PV1の機能解析
  森田将之; 高島英造; 長岡ひかる; KANOI Bernard N.; 伊藤大輔; THONGKUKIATKUL Amporn; 李智媛; 徳永和明; 飯村忠浩; 鳥居本美; 坪井敬文, 日本寄生虫学会大会プログラム・抄録集, 86th, 2017
• 歯科インプラント埋入後に新生されたオステオンの構造特性
  是澤和人; 松永智; 小高研人; 森田純晴; 廣内英智; 飯村忠浩; 阿部伸一, Journal of Oral Biosciences Supplement (Web), 2017, 2017
• エピゲノム制御因子Uhrf1によるDNAメチル化制御を介した軟骨分化・骨格形成
  山下美智子; 山下美智子; 井上和樹; 井上和樹; 佐伯法学; 佐伯法学; 榊原伊織; LEE Jiwon; 大塚まき; 亀井義明; 五十嵐勝秀; 高田泰次; 飯村忠浩; 飯村忠浩; 今井祐記, 日本骨代謝学会学術集会プログラム抄録集, 35th, 163, 163, 2017
  (一社)日本骨代謝学会, Japanese
• HIV治療薬標的分子CCR5の破骨細胞機能分化における必須の機能-CCR5を標的としたHIV治療は骨吸収性疾患に対してもメリットをもたらす可能性がある
  LEE Ji-Won; 上原俊介; 小林泰浩; 山口朗; 今井祐記; 飯村忠浩; 飯村忠浩; 飯村忠浩; 飯村忠浩, 日本骨代謝学会学術集会プログラム抄録集, 35th, 186, 186, 2017
  (一社)日本骨代謝学会, Japanese
• FIB-SEMで探るコラーゲン細線維の集束化と骨細胞ネットワーク形成の関連について
  上岡寛; 橋本真奈; 長岡紀幸; 飯村忠浩; 大嶋佑介; 原徹, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 122nd, 2017
• 骨細胞ネットワーク形成に与えるコラーゲン線維構築の関与
  上岡寛; 橋本真奈; 長岡紀幸; 大嶋佑介; 飯村忠浩; 原徹, Journal of Oral Biosciences Supplement (Web), 2017, ROMBUNNO.US10‐4 (WEB ONLY), 2017
  Japanese
• 破骨細胞由来のLIFはsclerostinの発現低下を介して,骨形成を促進する
  小出雅則; 小林泰浩; 山下照仁; 上原俊介; 中村美どり; 平岡行博; 尾崎友輝; 飯村忠浩; 高橋直之; 宇田川信之; 宇田川信之, Journal of Oral Biosciences Supplement (Web), 2017, ROMBUNNO.O2‐D8 (WEB ONLY), 2017
  Japanese
• 破骨細胞由来のLIFは骨細胞におけるsclerostinの発現を低下させ,骨形成を促進する
  小出雅則; 小林泰浩; 山下照仁; 上原俊介; 尾崎友輝; 飯村忠浩; 中村美どり; 保田尚孝; 高橋直之; 宇田川信之, 日本骨代謝学会学術集会プログラム抄録集, 35th, 174, 174, 2017
  (一社)日本骨代謝学会, Japanese
• アプライド・イメージング 先進イメージングによる生命医学のブレークスルー マルチモーダル超解像法を用いた骨系細胞の機能解析
  飯村 忠浩; 李 智媛, Journal of Oral Biosciences Supplement, 2016, 70, 70, Sep. 2016
  (一社)歯科基礎医学会, Japanese
• 硬組織の形態・機能解析のための光学顕微鏡のマルチモードな活用法 微分干渉から先進レーザー顕微鏡の応用例
  飯村 忠浩; 李 智媛, Journal of Oral Biosciences Supplement, 2016, 86, 86, Sep. 2016
  (一社)歯科基礎医学会, Japanese
• 機能と形態の統合的アプローチによる血管・唾液腺・骨の共創的研究 機能的生細胞イメージングとオミクス解析による破骨細胞調節機能の解明(Unveiling novel regulatory mechanisms of osteoclasts by combinatorial approaches of functional live-imaging and molecular omics)
  李 智媛; 飯村 忠浩, Journal of Oral Biosciences Supplement, 2016, 139, 139, Sep. 2016
  (一社)歯科基礎医学会, English
• 骨形成の超解像イメージング解析
  飯村忠浩; 飯村忠浩, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 121st, 2016
• 2光子励起顕微鏡を用いたSHGイメージングによる変形性関節症の診断的定量評価法
  清松悠; 大嶋佑介; 大嶋佑介; 斎藤卓; 疋田温彦; 宮崎剛; 今村健志; 今村健志; 飯村忠浩; 三浦裕正; 三浦裕正, 日本軟骨代謝学会プログラム・抄録集, 29th, 2016
• マルチモーダル超解像法を用いた骨系細胞の機能解析
  飯村忠浩; 飯村忠浩; 飯村忠浩; 飯村忠浩; LEE J-W, Journal of Oral Biosciences Supplement (Web), 2016, 2016
• 硬組織の形態・機能解析のための光学顕微鏡のマルチモードな活用法-微分干渉から先進レーザー顕微鏡の応用例-
  飯村忠浩; 飯村忠浩; 飯村忠浩; 飯村忠浩; LEE J-W, Journal of Oral Biosciences Supplement (Web), 2016, 2016
• 副甲状腺ホルモン:PTHの分子シグナルと薬理効果
  飯村忠浩, 日本骨代謝学会学術集会プログラム抄録集, 34th, 139, 139, 2016
  (一社)日本骨代謝学会, Japanese
• 医理工融合研究センターにおける学際的研究の推進~異分野融合は異文化融合~
  飯村忠浩, 日本口腔科学会学術集会プログラム・抄録集, 70th, 2, 105, 106, 2016
  (NPO)日本口腔科学会, Japanese
• 副甲状腺ホルモンの間欠投与による骨形成促進作用について
  小倉健司; 小倉健司; 飯村忠浩; 山口朗; 山口朗; 森山啓司, 日本矯正歯科学会大会プログラム・抄録集, 75th, 201, 201, 2016
  (公社)日本矯正歯科学会, Japanese
• 超解像顕微鏡の生物医学的応用「ここまで微細に,こんな綺麗に見える!」
  飯村忠浩; 飯村忠浩; 飯村忠浩, 日本組織細胞化学会総会・学術集会講演プログラム・予稿集, 57th, 41, 41, 2016
  日本組織細胞化学会, Japanese
• タンパク質輸送機構に関連すると考えられる熱帯熱マラリア原虫デンスグラニュールタンパク質PV1の機能解析
  森田将之; 高島英造; 長岡ひかる; 伊藤大輔; BERNARD N. Kanoi; 中田貴敬; 西莉菜; 飯村忠浩; 鳥居本美; 坪井敬文, 日本寄生虫学会大会プログラム・抄録集, 85th, 2016
• 短期間の副甲状腺ホルモンの間欠投与は海綿骨と皮質骨で異なるメカニズムにより骨形成を促進する
  小倉健司; 小倉健司; 飯村忠浩; 牧野祐司; 杉江綾乃; 高倉綾; 高尾亮子; 石津谷俊則; 森山啓司; 山口朗; 山口朗, 日本骨代謝学会学術集会プログラム抄録集, 34th, 212, 212, 2016
  (一社)日本骨代謝学会, Japanese
• 骨細胞ネットワーク形成に与えるコラーゲン線維集束化の関与
  橋本真奈; 長岡紀幸; 飯村忠浩; 大嶋佑介; 原徹; 上岡寛, Journal of Oral Biosciences Supplement (Web), 2016, 2016
• 生体機能の多次元バイオイメージング ライブイメージングと数理計測で見る脊椎動物の発生ボディプランの調節機構
  飯村 忠浩, Journal of Oral Biosciences Supplement, 2015, 55, 55, Sep. 2015
  (一社)歯科基礎医学会, Japanese
• 骨の細胞イメージング-a new horizon-骨のメカニズムをどこまで観ることができるか 蛍光細胞イメージングと骨形態計測で見る骨代謝におけるケモカインシグナルの役割
  李 智媛; 飯村 忠浩, Journal of Oral Biosciences Supplement, 2015, 100, 100, Sep. 2015
  (一社)歯科基礎医学会, Japanese
• 骨代謝関連細胞ネットワークin vitro再構築系の2光子イメージング
  疋田温彦; 飯村忠浩; 大嶋佑介; 齋藤卓; 山本真; 今村健志, 日本骨形態計測学会雑誌, 25, 2, S71, S71, 15 May 2015
  日本骨形態計測学会, Japanese
• 第二次高調波発生(SHG)イメージングによる軟骨変性の定量的・組織学的評価法の開発
  大嶋佑介; 清松悠; 齋藤卓; 三浦裕正; 飯村忠浩; 今村健志, 日本骨形態計測学会雑誌, 25, 2, S45, S45, 15 May 2015
  日本骨形態計測学会, Japanese
• マルチスケール画像解析の骨生物医学への応用展開
  飯村忠浩; 飯村忠浩; 飯村忠浩; 飯村忠浩; 李智媛, 日本骨形態計測学会雑誌, 25, 2, S46, S46, 2015
  日本骨形態計測学会, Japanese
• ライブイメージングと数理計測で見る脊椎動物の発生ボディプランの調節機構
  飯村忠浩; 飯村忠浩; 飯村忠浩, Journal of Oral Biosciences Supplement (Web), 2015, 2015
• 蛍光細胞イメージングと骨形態計測で見る骨代謝におけるケモカインシグナルの役割
  LEE J; 飯村忠浩; 飯村忠浩; 飯村忠浩, Journal of Oral Biosciences Supplement (Web), 2015, 2015
• In vivo detection of cancer cells with immunoconjugated fluorescent probes by macro zoom microscopy and two-photon microscopy
  Shigehiro Koga; Yusuke Oshima; Atsuhiko Hikita; Koichi Sato; Motohira Yoshida; Yuji Yamamoto; Tadahiro Iimura; Yuji Watanabe; Takeshi Imamura, REPORTERS, MARKERS, DYES, NANOPARTICLES, AND MOLECULAR PROBES FOR BIOMEDICAL APPLICATIONS VII, 9339, 2015, [Peer-reviewed]
  English
• 骨吸収の促進は骨細胞におけるsclerostinの発現を低下させ,骨形成を促進する
  小出雅則; 小林泰浩; 山下照仁; 上原俊介; 尾崎友輝; 飯村忠浩; 中村美どり; 保田尚孝; 高橋直之; 宇田川信之; 宇田川信之, 日本骨代謝学会学術集会プログラム抄録集, 33rd, 181, 181, 2015
  (一社)日本骨代謝学会, Japanese
• 骨吸収の促進は骨細胞におけるsclerostinの発現を低下させ,Wnt/β‐cateninシグナルを促進する
  小出雅則; 小林泰浩; 山下照仁; 上原俊介; 飯村忠浩; 中村美どり; 高橋直之; 宇田川信之; 宇田川信之, Journal of Oral Biosciences Supplement (Web), 2015, ROMBUNNO.O2‐E2 (WEB ONLY), 2015
  Japanese
• 中軸骨格形態形成の観察と計算モデル
  齋藤 卓; 今村 健志; 飯村 忠浩, 日本骨形態計測学会雑誌, 24, 2, S105, S105, May 2014
  日本骨形態計測学会, Japanese
• Evaluation of cartilage based on second harmonic generation microscopy.
  Hiroshi Kiyomatsu; Takeshi Imamura; Atsuhiko Hikita; Tadahiro Iimura; Tsuyoshi Miyazaki; Yusuke Ohsima; Takashi Saitou; Hiromasa Miura, JOURNAL OF BONE AND MINERAL RESEARCH, 29, S235, S235, Feb. 2014
  English, Summary international conference
• 骨細胞の機能的3次元蛍光計測
  飯村忠浩, 日本骨形態計測学会雑誌, 24, 2, S55, S55, 2014
  日本骨形態計測学会, Japanese
• ケモカイン受容体CX3CR1の骨代謝における役割
  飯村忠浩; 飯村忠浩; 飯村忠浩; 飯村忠浩; 山口朗, 日本骨代謝学会学術集会プログラム抄録集, 32nd, 144, 144, 2014
  (一社)日本骨代謝学会, Japanese
• DMP1は骨細胞におけるFGF23産生の抑制因子である-蛍光3次元形態計測と細胞生物学的アプローチ-
  李智媛; 李智媛; 山口朗; 飯村忠浩; 飯村忠浩; 飯村忠浩; 飯村忠浩, 日本骨代謝学会学術集会プログラム抄録集, 32nd, 290, 290, 2014
  (一社)日本骨代謝学会, Japanese
• ラマン分光法および多変量解析による骨粗鬆症ラットの骨基質の解析
  大嶋佑介; 飯村忠浩; 齋藤卓; 今村健志, 日本分子生物学会年会プログラム・要旨集(Web), 37th, 1P-0994 (WEB ONLY), 2014
  Japanese
• 椎間板軟骨の初期発生におけるPax1の役割
  牧野祐司; 牧野祐司; 金子和夫; 山口朗; 飯村忠浩, 日本骨代謝学会学術集会プログラム抄録集, 31st, 103, 103, 2013
  (一社)日本骨代謝学会, Japanese
• 中軸骨格の形態発生の分子基盤-蛍光イメージングで見るボディプランと骨形態疾患-
  飯村忠浩, 松本歯学, 39, 2, 171, 171, 2013
  松本歯科大学学会, Japanese
• 3次元蛍光イメージング法による骨発達の分子メカニズムの探索
  飯村忠浩, 日本口腔科学会雑誌, 62, 1, 37, 37, 2013
  (NPO)日本口腔科学会, Japanese
• 椎間板発生における転写因子Pax1の役割の検討
  牧野祐司; 牧野祐司; 玉村禎宏; 金子和夫; 山口朗; 飯村忠浩, 日本整形外科学会雑誌, 87, 8, S1480, S1480, 2013
  (公社)日本整形外科学会, Japanese
• 医療応用をめざしたラマン分光イメージングシステムの開発
  大嶋佑介; 大嶋佑介; 大嶋佑介; 飯村忠浩; 飯村忠浩; 疋田温彦; 疋田温彦; 今村健志; 今村健志, 最先端光計測とライフサイエンスの近未来-バイオ・ラマン2017(3) 理研シンポジウム 平成25年, 2013
• 異骨症モデルマウスを用いた骨格発生と病態の時空間的解析
  飯村 忠浩, Journal of Oral Biosciences Supplement, 2012, 76, 76, Sep. 2012
  (一社)歯科基礎医学会, Japanese
• 蛍光3次元イメージング形態計測によるSclerostinの時空間的発現変化と生後骨発達における役割
  渡辺 高; 山口 朗; 飯村 忠浩, Journal of Oral Biosciences Supplement, 2012, 122, 122, Sep. 2012
  (一社)歯科基礎医学会, Japanese
• 蛍光3次元イメージング形態計測による頭頂骨と長管骨の骨細胞ネットワークの構造の定量的比較
  飯村忠浩; 飯村忠浩; 山口朗; 山口朗, 日本骨形態計測学会雑誌, 22, 1, S76, S76, 2012
  日本骨形態計測学会, Japanese
• 口腔癌による骨破壊では間質細胞と癌細胞の両者が産生するRANKLが重要である
  佐藤潔; 李智媛; 飯村忠浩; 保田尚孝; 伊東昌子; 小村健; 山口朗, 日本骨代謝学会学術集会プログラム抄録集, 30th, 218, 218, 2012
  (一社)日本骨代謝学会, Japanese
• モデルマウスを用いた脊椎肋骨異骨症および脊椎胸郭異骨症の病因解析
  牧野祐司; 牧野祐司; 高橋雄; 玉村禎宏; 田辺里枝子; 祓川摩有; 五関(曽根)正江; 波多賢二; 米田俊之; 菅野純; 金子和夫; 山口朗; 山口朗; 飯村忠浩; 飯村忠浩, 日本骨形態計測学会雑誌, 22, 1, S117, S117, 2012
  日本骨形態計測学会, Japanese
• 蛍光3次元イメージング形態計測によるSclerostinの時空間的発現変化の定量的解析と生後骨発達における役割
  飯村忠浩; 飯村忠浩; 渡辺高; 渡辺高; 渡辺高; 牧野祐司; 牧野祐司; 牧野祐司; 金子和夫; 天笠光雄; 山口朗; 山口朗, 日本骨形態計測学会雑誌, 22, 1, S78, S78, 2012
  日本骨形態計測学会, Japanese
• 脊椎肋骨異骨症・脊椎胸郭異骨症の発生病因-Mesp2-nullマウスの解析-
  牧野祐司; 高橋雄; 玉村禎宏; 田辺里枝子; 祓川摩有; 五関(曽根)正江; 菅野純; 金子和夫; 山口朗; 飯村忠浩, 日本整形外科学会雑誌, 86, 8, S1291, S1291, 2012
  (公社)日本整形外科学会, Japanese
• 骨芽細胞分化と骨形成における骨芽細胞特異的転写因子の細胞内局在変化-蛍光イメージングによる解析-
  渡辺高; 渡辺高; 渡辺高; 天笠光雄; 山口朗; 飯村忠浩; 飯村忠浩, 日本口腔科学会雑誌, 61, 1, 172, 172, 2012
  (NPO)日本口腔科学会, Japanese
• 3次元蛍光イメージグと計測で見る骨格の発生異常と骨の発達のメカニズム
  飯村忠浩; 山口朗, 日本骨代謝学会学術集会プログラム抄録集, 30th, 138, 138, 2012
  (一社)日本骨代謝学会, Japanese
• 異骨症モデルマウスを用いた骨格発生と病態の時空間的解析
  飯村忠浩, Journal of Oral Biosciences Supplement (Web), 2012, 2012
• 蛍光3次元イメージング形態計測によるSclerostinの時空間的発現変化と生後骨発達における役割
  渡辺高; 山口朗; 山口朗; 飯村忠浩; 飯村忠浩, Journal of Oral Biosciences Supplement (Web), 2012, 2012
• 進行性下顎頭吸収の診断基準策定とその治療に関する研究 血液,および尿検体からのマーカー検索 PCR患者の治療過程の報告 CCR5ノックアウトマウスの解析
  丸岡豊; 桂川陽三; 今井英樹; 大塚亮; 山本健二; 星野昭芳; 飯村忠浩; 新保卓郎; 山崎力; 叶谷文秀, 進行性下顎頭吸収の診断基準策定とその治療に関する研究 平成23年度 総括・分担研究報告書, 19, 31, 2012
  Japanese
• 進行性下顎頭吸収の診断基準策定とその治療に関する研究 全国の医療機関に対する疾患の実態把握調査 血液,および尿検体からのマーカー検索 PCR患者の治療過程 CCR1,およびCCR5ノックアウトマウスの解析 データベースに基づく関節リウマチ適正治療の調査
  丸岡豊; 三森明夫; 桂川陽三; 今井英樹; 大塚亮; 山本健二; 星野昭芳; 飯村忠浩; 新保卓郎; 山崎力, 進行性下顎頭吸収の診断基準策定とその治療に関する研究 平成22-23年度 総合研究報告書, 37, 71, 2012
  Japanese
• 低分子量Gタンパク質Cdc42は四肢形成における軟骨分化と肢芽指間部の細胞死に必須である
  相澤怜; 山田篤; 鈴木大; 飯村忠浩; 山本剛; 山口朗; 山本松男; 上條竜太郎, 日本骨代謝学会学術集会プログラム抄録集, 30th, 211, 211, 2012
  (一社)日本骨代謝学会, Japanese
• Cdc42は四肢形成における軟骨形成と肢芽指間域のアポトーシスを制御する
  相澤怜; 山田篤; 鈴木大; 塚崎雅之; 山本剛; 飯村忠浩; 山口朗; 山本松男; 上條竜太郎, Journal of Oral Biosciences, 53, Supplement, 138, 138, 01 Sep. 2011
  (一社)歯科基礎医学会, Japanese
• Hox genes control the axis elongation process in chicken embryo
  Nicolas Denans; Tadahiro Iimura; Olivier Pourquie, DEVELOPMENTAL BIOLOGY, 356, 1, 235, 235, Aug. 2011
  English, Summary international conference
• 骨の成長と代謝における概日リズムの働き 蛍光イメージングによるアプローチと展望
  中根 綾子; 沼野 利佳; 佐藤 博紀; 高木 裕三; 山口 朗; 飯村 忠浩, 日本骨形態計測学会雑誌, 21, 1, 41, 50, May 2011
  日本骨形態計測学会, Japanese
• Current topics in fluorescent bio-imaging in skeletal development and bone biology
  飯村忠浩; 飯村忠浩; 中根綾子; 中根綾子; 中根綾子; 姫野彰子; 姫野彰子; 姫野彰子; 杉山真由; 山口朗; 山口朗, 日本骨形態計測学会雑誌, 21, 1, 17, 24, 2011
  日本骨形態計測学会, Japanese
• 矯正力による歯の移動における骨細胞の役割
  松本力; 松本力; 飯村忠浩; 山口朗, 日本骨形態計測学会雑誌, 21, 2, S63, S63, 2011
  日本骨形態計測学会, Japanese
• 脊椎肋骨異骨症(SCDO)2型モデルMesp2(-/-)マウスの椎体における骨・軟骨組織の発生・分化過程の解析-分節時計は椎体と椎間板の規則的空間配置に必須である-
  牧野祐司; 牧野祐司; 田辺里枝子; 波多賢二; 五関(曽根)正江; 金子和夫; 山口朗; 飯村忠浩, 日本骨代謝学会学術集会プログラム抄録集, 29th, 223, 223, 2011
  (一社)日本骨代謝学会, Japanese
• 進行性下顎頭吸収の診断基準策定とその治療に関する研究 基礎班:骨組織におけるケモカイン受容体CCR1機能の解析
  星野昭芳; 飯村忠浩; 松島綱治; 上羽悟史; 山本健二, 進行性下顎頭吸収の診断基準策定とその治療に関する研究 平成22年度 総括・分担研究報告書, 2011
• 定量的in situ蛍光イメージングによる骨芽細胞特異的転写因子の骨組織内での分布と細胞内局在の変化の観察
  渡辺高; 渡辺高; 天笠光雄; 山口朗; 飯村忠浩, 日本骨形態計測学会雑誌, 21, 2, S102, S102, 2011
  日本骨形態計測学会, Japanese
• 進行性下顎頭吸収の診断基準策定とその治療に関する研究 全国の医療機関に対する疾患の実態把握調査 血液,および尿検体からのマーカー検索
  丸岡豊; 三森明夫; 桂川陽三; 今井英樹; 大塚亮; 飯村忠浩; 新保卓郎; 山崎力, 進行性下顎頭吸収の診断基準策定とその治療に関する研究 平成22年度 総括・分担研究報告書, 17, 39, 2011
  Japanese
• 生体イメージングの現状と応用 脊椎動物の発生におけるライブイメージング
  飯村 忠浩, Cytometry Research, 20, Suppl., 58, 58, Jun. 2010
  (一社)日本サイトメトリー学会, Japanese
• 骨成長における概日リズムの可視化
  中根 綾子; 姫野 彰子; 沼野 利佳; 高木 裕三; 山口 朗; 飯村 忠浩, 日本骨形態計測学会雑誌, 20, 1, S81, S81, May 2010
  日本骨形態計測学会, Japanese
• 発生遺伝学から脊椎骨異形成症の責任遺伝子の同定へのプロセス
  飯村忠浩, 日本顎変形症学会雑誌, 20, 2, 78, 78, 2010
  (NPO)日本顎変形症学会, Japanese
• 蛍光イメージングと計測による細胞機能の探索
  飯村忠浩, 日本骨代謝学会学術集会プログラム抄録集, 28th, 119, 119, 2010
  (一社)日本骨代謝学会, Japanese
• 骨組織の細胞機能イメージング法による新規の機能解析
  飯村忠浩, Journal of Oral Biosciences, 52, Supplement, 59, 59, 2010
  (一社)歯科基礎医学会, Japanese
• 骨代謝におけるケモカイン受容体CCR1の機能解析
  星野昭芳; 星野昭芳; 飯村忠浩; 山本健二; 山口朗, 日本骨代謝学会学術集会プログラム抄録集, 28th, 164, 164, 2010
  (一社)日本骨代謝学会, Japanese
• 蛍光イメージングによる骨細胞の形態・機能分化の計測
  姫野彰子; 姫野彰子; 中根綾子; 中根綾子; 和泉雄一; 山口朗; 飯村忠浩, 日本骨形態計測学会雑誌, 20, 1, S76, S76, 2010
  日本骨形態計測学会, Japanese
• 進行性下顎頭吸収の診断基準策定とその治療に関する研究 全国の医療機関に対する疾患の実態把握調査診断基準確立のためのマーカー探索 CCR1,CCR5ノックアウトマウスの顎関節の微細構造解析
  丸岡豊; 小村健; 森山啓司; 三森明夫; 今井英樹; 大塚亮; 飯村忠浩; 新保卓郎; 山崎力, 進行性下顎頭吸収の診断基準策定とその治療に関する研究 平成21年度 総括・分担研究報告書, 11, 30, 2010
  Japanese
• 骨のin vivo蛍光イメージングの現状と展望
  飯村忠浩, 日本骨形態計測学会雑誌, 19, 1, S33, S33, 2009
  日本骨形態計測学会, Japanese
• 口腔扁平上皮癌による骨破壊メカニズム
  栢森高; 姫野彰子; 飯村忠浩; 山口朗, 癌と骨病変研究会抄録集, 12th, 2009
• Phosphate starvation enhances bone morphogenetic protein gene expression in a cultured mouse marrow stromal cell line ST2
  L Mizoi; Ezawa, I; T Iimura; M Goseki-Sone, BONE, 32, 5, S111, S111, May 2003
  English, Summary international conference
• 頭蓋顔面骨格の形の分化とパラドックス (2) 頭部中はい葉は骨の形づくりをサポートする
  飯村忠浩, Quintessence, 21, 12, 2002
• rhFGF4 promotes ectopic bone formation induced by rhBMP2.
  K Ikeda; S Kuroda; S Oida; T Iimura; WR Duarte; K Ohya; Ishikawa, I; S Kasugai, JOURNAL OF BONE AND MINERAL RESEARCH, 15, S257, S257, Sep. 2000
  English, Summary international conference
• アトラス特別講座 最終回 (12) 頭蓋顎顔面の分子発生学 最終回 遺伝子DNAは発生をどう説明するのか? 遺伝子・かたちの変化・そして時間
  飯村忠浩; 江藤一洋, Quintessence, 19, 12, 2000
• Role of S100A4 calcium-binding protein in mineralized nodule formation.
  WR Duarte; S Kasugai; T Iimura; K Takenaga; K Ikeda; K Ohya; Ishikawa, I, JOURNAL OF DENTAL RESEARCH, 79, 221, 221, 2000
  English, Summary international conference
• Expression of FGFs and their receptors during BMP-2 induced bone formation
  K Ikeda; S Kasugai; WR Duarte; S Kuroda; S Oida; T Iimura; K Ohya; Ishikawa, I, JOURNAL OF DENTAL RESEARCH, 79, 218, 218, 2000
  English, Summary international conference
• 哺乳類顎顔面の発生におけるBMP-Msx遺伝子カスケードの役割
  飯村 忠浩, 解剖学雑誌, 74, 5, 587, 587, Oct. 1999
  (一社)日本解剖学会, Japanese
• Effects of high extracellular calcium on the expression of the calcium-binding protein S100A4 by osteoblastic MC3T3-E1 cells.
  WR Duarte; S Kasugai; K Takenaga; K Ikeda; T Iimura; K Ohya; Ishikawa, I, JOURNAL OF BONE AND MINERAL RESEARCH, 14, S220, S220, Sep. 1999
  English, Summary international conference
• Anabolic effects of amino-terminally truncated rhFGF4 on bone: Histomorphometric analysis.
  S Kuroda; S Kasugai; H Kondo; S Yoneda; D Ito; H Mukohyama; S Oida; T Iimura; K Ohya; T Ohyama, JOURNAL OF BONE AND MINERAL RESEARCH, 14, S330, S330, Sep. 1999
  English, Summary international conference
• 哺乳類顔面の発生におけるBMP-Msx遺伝子カスケードの役割
  飯村 忠浩; 今井 元; 青戸 一司; 江藤 一洋, 歯科基礎医学会雑誌, 41, 5, 469, 469, 20 Aug. 1999
  歯科基礎医学会, Japanese
• Amino-terminally truncated rhFGF4の作製とその骨量増加作用
  黒田 真司; 春日井 昇平; 大井田 新一郎; 飯村 忠浩; 向山 仁; 近藤 尚知; 米田 哲; 伊藤 大輔; 大谷 啓一; 大山 喬史, 日本骨代謝学会雑誌, 17, 2, 210, 210, Jun. 1999
  (一社)日本骨代謝学会, Japanese
• ラット初期胚におけるN-Deacetylase/N-Sulfotransferase(ND/NS)の発現
  池田 美子; 飯村 忠浩; 石黒 聡子; 江藤 一洋; 柳下 正樹, 歯科基礎医学会雑誌, 40, 抄録, 450, 450, Sep. 1998
  (一社)歯科基礎医学会, Japanese
• Differentiation and induction of head neural crest cell of mammals embryo to cartilage by BMP.
  飯村忠浩; 今井元; 石黒聡子; 江藤一洋, 日本発生生物学会大会発表要旨集, 31st, 158, 158, 1998
  日本発生生物学会, Japanese
• Contribution of fore-gut endoderm to tooth germ formation.
  今井元; 飯村忠浩; 青戸一司; 池田正明; 江藤一洋, 日本発生生物学会大会発表要旨集, 31st, 1998
• Detection of S100A4 mRNA and protein in the periodontal ligament
  WR Duarte; S Kasugai; T Iimura; S Oida; K Takenaga; K Ohya; Ishikawa, I, JOURNAL OF DENTAL RESEARCH, 77, 761, 761, 1998
  English, Summary international conference
• 卵巣摘出ラットにおける小腸型アルカリホスファターゼの遺伝子発現について
  松本 裕子; 山本 麻子; 飯村 忠浩, Journal of Nutritional Science and Vitaminology, 43, 5, 529, 539, Oct. 1997
  卵巣摘出(OVX)により,骨中カルシウム・リン含量及び骨強度は有意に減少した.小腸ALPの酵素活性は,OVXにより減少したが,同じ小腸酵素であるsucraseやtrehalase活性には影響がなかった.更に小腸型ALP遺伝子であるRTIN-1とRTIN-2の2種類のmRNAについてRT-PCR法により,遺伝子発現を調べた結果,OVXラットにおいてRTIN-1のmRNAの発現レベルは変化していなかったが,RTIN-2のmRNA発現レベルが顕著に低下していた.以上のことからOVXにより,RTIN-2型ALPのmRNAの発現が抑制され,小腸でのCa・Pの代謝において間接的な影響を及ぼしている可能性が示唆された, (公社)日本栄養・食糧学会, English
• 頭蓋顔面の発生におけるBMP遺伝子群の発現と機能
  飯村 忠浩, 歯科基礎医学会雑誌, 39, 抄録, 467, 467, Sep. 1997
  (一社)歯科基礎医学会, Japanese
• Chondrogenesis accelerating effect by swimming ( water motion ).
  山本麻子; 五関正江; 丸岡豊; 旭久美子; 飯村忠浩; 大井田新一郎; 江沢郁子, 日本骨代謝学会雑誌, 15, 2, 1997
• Functional analysis of BMP and Msx genes during Craniofacial Development.
  飯村忠浩; 石黒聡子; 今井元; 大井田新一郎; 江藤一洋, 日本分子生物学会年会プログラム・講演要旨集, 20th, 1997
• Cloning and expression of S100 family gene in periodontal ligament.
  WR Duarte; S Kasugai; T Iimura; S Oida; K Ohya; Ishikawa, I, JOURNAL OF DENTAL RESEARCH, 76, 3504, 3504, 1997
  English, Summary international conference
• Pax-6ミュータントラットにおける体性運動性脳神経の発生異常
  大隈-山下 典子; 飯村 忠浩; 江藤 一洋, 日本分子生物学会年会プログラム・講演要旨集, 19, 529, 529, 01 Aug. 1996
  Japanese
• TGF-βスーパーファミリーによるアメロジェニン遺伝子の発現
  大井田 新一郎; 田村 正人; 飯村 忠浩; 鈴木 ミチ子; 下川 仁弥太, 日本分子生物学会年会プログラム・講演要旨集, 19, 825, 825, 01 Aug. 1996
  Japanese, Summary national conference
• Altered expression patterns of developmental genes in rat Small eye embryos.
  大隅典子; 石黒聡子; 飯村忠浩; 大井田新一郎; YU R; 梅園和彦; 江藤一洋, 日本分子生物学会年会プログラム・講演要旨集, 18th, 204, Nov. 1995
  Japanese
• PROSTAGLANDIN-E2 EFFECTS ON MINERALIZED-TISSUE FORMATION IN-VITRO DEPEND ON THE CELL STAGE OF OSTEOBLASTIC LINEAGE
  S KASUGAI; S OZAWA; H KONDO; H MUKOHYAMA; S OIDA; T IIMURA; N ARAI; K OHYA, JOURNAL OF BONE AND MINERAL RESEARCH, 10, S420, S420, Aug. 1995
  English, Summary international conference
• Molecuiar diagnosis of hypophosphatasia.
  五関正江; 折茂英生; 中島英逸; 高木裕三; 飯村忠浩; 佐藤清二; 真柳秀昭; 島田隆; 大井田新一郎, 日本分子生物学会年会プログラム・講演要旨集, 18th, 1995
• PRODUCTION OF FUNCTIONAL HUMAN BONE MORPHOGENETIC PROTEIN-2 USING A BACULOVIRUS SYSTEM
  Y MARUOKA; S OIDA; T IIMURA; K TAKEDA; ASAHINA, I; S ENOMOTO; S SASAKI, JOURNAL OF DENTAL RESEARCH, 74, 568, 568, 1995
  English, Summary international conference
• CHANGES IN HOMEOBOX-CONTAINING GENE-EXPRESSION DURING ECTOPIC BONE-FORMATION INDUCED BY BONE MORPHOGENETIC PROTEIN
  T IIMURA; S OIDA; K TAKEDA; Y MARUOKA; S SASAKI, JOURNAL OF DENTAL RESEARCH, 74, 480, 480, 1995
  English, Summary international conference
• EXPRESSION OF BONE MORPHOGENETIC PROTEINS IN ODONTOBLASTS
  S OIDA; K TAKEDA; T IIMURA; Y MARUOKA; H SIMOKAWA; S SASAKI, JOURNAL OF DENTAL RESEARCH, 74, 568, 568, 1995
  English, Summary international conference
• 骨組織の発生・分化におけるホメオボックスとBMP
  飯村忠浩; 大井田新一郎; 下川仁弥太, 口腔病学会雑誌, 62, 4, 580, 580, 1995
  口腔病学会, Japanese
• BMPの異所性軟骨・骨誘導過程におけるホメオボックス遺伝子発現の変化
  飯村 忠浩, 歯科基礎医学会雑誌, 36, 抄録, 83, 83, Sep. 1994
  (一社)歯科基礎医学会, Japanese
• バキュロウィルス(AcNPV)-昆虫細胞系を用いたリコンビナントヒト骨形成タンパク質(rhBMP-2)の生産
  丸岡豊; 大井田新一郎; 飯村忠浩; 武田弘資; 五関正江; 榎本昭二; 佐々木哲, 日本骨代謝学会雑誌, 12, 2, 1994
• BMPの異所性軟骨・骨誘導過程におけるホメオボックス遺伝子の発現
  飯村忠浩; 大井田新一郎; 武田弘資; 丸岡豊; 佐々木哲, 日本骨代謝学会雑誌, 12, 2, 112, 112, 1994
  (一社)日本骨代謝学会, Japanese
• 異所性軟骨・骨誘導におけるラットBMP-6遺伝子の発現とクローニング
  大井田新一郎; 飯村忠浩; 武田弘資; 丸岡豊; 佐々木哲, 日本骨代謝学会雑誌, 12, 2, 1994
• Altered expression of Hox and Msx homeobox genes during ectopic bone formation induced by bone morphogenetic proteins
  Iimura Tadahiro; Oida Shinichiro; Takeda Kohsuke; Maruoka Yutaka; Sasaki Satoshi, Journal of hard tissue biology, 3, 1, 51, 51, 1994
  日本硬組織研究技術学会, Japanese
• ヒト骨肉腫由来細胞株(MG-63)の分化におけるアクチビンAの生物学的作用について
  飯村 忠浩, 歯科基礎医学会雑誌, 35, 抄録, 180, 180, Sep. 1993
  (一社)歯科基礎医学会, Japanese
• AcNPV-昆虫細胞発現系を用いたヒトBMP-4の生産
  丸岡豊; 大井田新一郎; 飯村忠浩; 武田弘資; 佐々木哲; 榎本昭二, 日本分子生物学会年会プログラム・講演要旨集, 16th, 1993
• Production of Human BMP-4 in E. coli Expression System
  Oida Shinichiro; Maruoka Yutaka; Iimura Tadahiro; Takeda Kousuke; Sasaki Satosi, Journal of hard tissue biology, 2, 1, 45, 45, 1993
  日本硬組織研究技術学会, Japanese
• Cloning of human bone morphogenetic protein-2(hBMP-2) cDNA by PCR method.
  Maruoka Yutaka; Oida Shinichiro; Iimura Tadahiro; Nifuji Akira; Tamura Masato; Takeda Kohsuke; Sasaki Satoshi; Enomoto Shoji, Journal of Oral Biosciences, 35, 1, 64, 74, 1993
  In this study, we attempt to amplify the complementary DNAs (cDNAs) for human bone morphogenetic proteins (hBMPs), which have a strong activity of bone induction, from a cDNA library derived from a human osteosarcoma cell line MG63, using the polymerase chain reaction (PCR) method with two specific primers. Four recombinant clones, which encoded the mature region of hBMP-2, have been amplified. However, they did not contain termination codon at their 3′-terminus. PCR was performed again in order to ligate termination codon to one of the cDNAs. Sequence analysis revealed several mutations including one frameshift mutation. After elimination of the defected nucleotide moiety, a cDNA preparation encoding mature region of hBMP was obtained. It was confirmed that the amino acid sequence translated from this cDNA was identical to that of the mature region of hBMP-2., Japanese Association for Oral Biology, Japanese
• ヒト骨肉腫由来細胞株(MG-63)からのPCR法によるヒトアクチビン受容体のクローニング
  飯村 忠浩, 日本口腔科学会雑誌, 41, 5, 857, 857, Dec. 1992
  (NPO)日本口腔科学会, Japanese
• ヒト骨肉腫由来骨芽細胞様株(MG-63)からのヒトアクチビン受容体cDNAクローニングとその発現
  飯村 忠浩, 歯科基礎医学会雑誌, 34, 抄録, 128, 128, Sep. 1992
  (一社)歯科基礎医学会, Japanese
• 骨形成タンパク質(BMP)遺伝子のクローニングと細胞・組織での遺伝子発現
  大井田新一郎; 丸岡豊; 飯村忠浩; 武田弘資; 佐々木哲, 日本骨代謝学会雑誌, 10, 3, 1992
• ヒト骨肉腫由来骨芽細胞様株(MG-63)からのヒトアクチビン受容体cDNAクローニングとその発現
  飯村忠浩; 大井田新一郎; 石崎明; 丸岡豊; 武田弘資; 佐々木哲, 日本骨代謝学会雑誌, 10, 3, 139, 139, 1992
  (一社)日本骨代謝学会, Japanese
• 骨誘導因子(BMP)の骨芽細胞系細胞への影響
  桜井規雄; 石崎明; 大井田新一郎; 丸岡豊; 飯村忠浩; 三木貴司; 森正次; 朝比奈泉; 榎本昭二, 日本骨代謝学会雑誌, 10, 3, 1992

• 現代歯科薬理学 第7版
  分担執筆:飯村忠浩, 骨代謝と骨粗鬆症治療薬
  医歯薬出版, 15 Jan. 2024, 4263456777, 416, Japanese, [Contributor]
• 口腔組織・発生学（第3版）
  飯村忠浩, 蛍光顕微鏡と蛍光バイオイメージング
  医歯薬出版, Jan. 2024, 9784263456767, [Contributor]
• 最新の骨粗鬆症学 第2版
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  日本臨牀社, Jan. 2023, [Contributor]
• 愛媛大学「研究室からこんにちは！」10
  飯村 忠浩, 画像によるライブイメージングで発生生物学を研究・がん診断などの応用が期待できるHox遺伝子
  アトラス出版, 2016, [Contributor]
• 骨ペディア 骨疾患・骨代謝キーワード事典
  飯村 忠浩, 第6章 keyword 21 「ケモカイン」
  羊土社, 2015, [Contributor]
• 口腔組織・発生学 （改訂 新版）
  飯村 忠浩, 蛍光バイオイメージング
  医歯薬出版, 2015, [Contributor]
• Key Word 1996-97 炎症免疫系
  飯村 忠浩, Hox 遺伝子
  先端医学社, 1996, [Contributor]
• 実験医学別冊・骨粗鬆症-分子メカニズムから病態，診断，治療まで
  飯村 忠浩, 骨基質タンパク、ハイドロキシアパタイトと石灰化骨、類骨
  羊土社, 1995, [Contributor]

• 骨形成型骨粗鬆症治療薬：PTH１型受容体作動薬の基礎薬理学および臨床薬理学 -歯科医師として知っておきたいこと-
  飯村忠浩
  北海道医療大学先端研究推進センターセミナー, 19 Feb. 2025, Invited oral presentation
  [Invited]
• Pharmacological effects of PTH on bone pain
  Tadahiro Iimura
  XVIth Congress of the International Society of Bone Morphometry (ISBM 2024 9/30-10/3 Toronto), 02 Oct. 2024, English, Invited oral presentation
  30 Sep. 2024 - 03 Oct. 2024, [Invited]
• 骨代謝と薬理作用からみた顎骨の特殊性
  飯村忠浩
  第78回日本口腔科学会学術集会, 21 Jul. 2024, Invited oral presentation
  [Invited]
• 骨代謝改善薬のドラッグリポジショニング研究
  飯村忠浩
  第65回歯科基礎医学会学術大会, 18 Sep. 2023, Invited oral presentation
• 骨粗鬆症薬PTH 投与で折れにくい骨の形成を確認
  飯村忠浩
  日本歯科新聞, 27 Jul. 2021, Media report
• ネアンデルタール人の遺伝子がコロナ重症化の原因 リスク3倍、東アジアとアフリカはほとんどなし
  飯村忠浩
  朝日新聞出版 雑誌「AERA」, Nov. 2020, Media report
• 現代人に絶滅人類の遺伝子 ウイルス攻略へ敵か味方か
  飯村忠浩
  日本経済新聞, 17 Oct. 2020, Media report
• 感染 民俗差なし 遺伝子レベル 北大など分析
  飯村忠浩
  毎日新聞, 28 Sep. 2020, Media report
• 骨代謝疾患とドラッグリポジショニング研究
  飯村忠浩
  第62回歯科基礎医学会学術大会, 13 Sep. 2020, Invited oral presentation
• 口腔と全身の運動器薬理学研究
  飯村 忠浩
  北海道大学大学院歯学研究院セミナー, 12 Dec. 2018, Japanese
  [Domestic Conference]
• CC ケモカイン受容体のゲノム進化と骨代謝における病態機能解析
  飯村 忠浩
  日仏生物学会第 189 回例会, 01 Dec. 2018, Japanese
  [Domestic Conference]
• 骨組織・細胞の形態学的観察法−光学顕微鏡の基礎から論文映えする写真の撮り方−
  飯村 忠浩
  第36回日本骨代謝学会学術集会, 28 Jul. 2018, Japanese
  [Invited], [Domestic Conference]
• 骨Ontogenyの学際的理解の追求
  飯村 忠浩
  第36回日本骨代謝学会学術集会, 27 Jul. 2018, Japanese
  [Invited], [Domestic Conference]
• マルチモード蛍光観察による新規の骨代謝病態機能探索
  飯村 忠浩; 李 智媛
  第123回日本解剖学会総会・全国学術集会, 28 Mar. 2018, Japanese
  [Invited], [Domestic Conference]
• ドラッグ・リポジョショニングとプロテオ創薬 による骨代謝改善標的分子の探索
  飯村 忠浩
  第410/411回 北海道歯学会例会, 07 Dec. 2017
  [Invited]
• 超解像イメージングによる骨系細胞の微細構造と動態機能解析
  飯村 忠浩
  第44回日本臨床バイオメカニクス学会, 24 Nov. 2017, Japanese
  [Invited], [Domestic Conference]
• HIV治療標的分子CCR5の 骨代謝における役割
  飯村 忠浩
  遺伝子病制御研究所 血管生物学セミナー, 01 Nov. 2017
  [Invited]
• 運動器研究における組織・細胞の形態学的観察法−私なら、こうする−
  飯村 忠浩
  日本骨代謝学会 Skeletal Science Retreat 2017, 26 Oct. 2017, Japanese
  [Invited], [Domestic Conference]
• 骨代謝のマルチモード・イメージング解析
  飯村 忠浩
  日本顕微鏡学会 平成29年度関西支部特別講演会, 04 Oct. 2017, Japanese
  [Invited], [Domestic Conference]
• マウスジェネティクスを用いたHIV感染患者の骨病態解明
  飯村 忠浩; 李 智媛
  第59回歯科基礎医学会学術大会, 16 Sep. 2017, Japanese
  [Invited], [Domestic Conference]
• 骨粗鬆症治療薬の育薬研究
  飯村 忠浩
  第24回NPO法人 東京血管疾患研究所セミナー, 01 Jul. 2017, Japanese
  [Invited], [Domestic Conference]
• 産学および分野横断連携研究の取り組みと成果
  飯村 忠浩
  第71回日本口腔科学会学術集会, 28 Apr. 2017, Japanese
  [Invited], [Domestic Conference]
• 先進光学顕微鏡の骨形態計測法への応用展開
  飯村 忠浩
  第122回日本解剖学会総会・全国学術集会, 28 Mar. 2017, Japanese
  [Invited], [Domestic Conference]
• 超解像顕微鏡の生物医学応用「ここまで微細に、こんなに綺麗に見える！」
  飯村 忠浩
  第57回日本組織細胞化学会総会・学術集会, 04 Sep. 2016, Japanese
  [Invited], [Domestic Conference]
• 硬組織の形態・機能解析のための光学顕微鏡のマルチモードな活用法−微分干渉から先進レーザー顕微鏡の応用例−
  飯村 忠浩; 李 智媛
  第58回歯科基礎医学会学術大会, 25 Aug. 2016, Japanese
  [Invited], [Domestic Conference]
• マルチモーダル超解像法を用いた骨系細胞の機能解析
  飯村 忠浩; 李 智媛
  第58回歯科基礎医学会学術大会, 25 Aug. 2016, Japanese
  [Invited], [Domestic Conference]
• 副甲状腺ホルモン：PTHの分子シグナルと薬理効果
  飯村 忠浩
  第34回日本骨代謝学会学術集会 / 第3回アジア太平洋骨代謝学会議, 22 Jul. 2016, Japanese
  [Invited], [Domestic Conference]
• 副甲状腺ホルモン：PTHの基礎生物医学－最近の知見から－
  飯村 忠浩
  第35回長崎骨粗鬆症研究会, 29 Jun. 2016, English
  [Invited], [Domestic Conference]
• 医理工融合研究センターにおける学際的研究の推進−異分野融合は異文化融合−
  飯村 忠浩
  第70回日本口腔科学会学術集会, 16 Apr. 2016, Japanese
  [Invited], [Domestic Conference]
• 骨形成の超解像イメージング解析
  飯村 忠浩
  第121回日本解剖学会総会・全国学術集会, 28 Mar. 2016, Japanese
  [Invited], [Domestic Conference]
• ライブイメージングと数理計測で見る脊椎動物の発生ボディプランの調節機構
  飯村 忠浩
  第57回歯科基礎医学会学術大会, 12 Sep. 2015, Japanese
  [Invited], [Domestic Conference]
• 骨吸収の促進は骨細胞におけるsclerostinの発現を低下させ、Wnt/β-cateninシグナルを促進する
  小出 雅則; 小林 泰浩; 山下 照仁; 上原 俊介; 飯村 忠浩; 中村 美どり; 高橋 直之; 宇田川 信之; 保田 尚孝
  Journal of Oral Biosciences Supplement, Sep. 2015, Japanese
• 骨の細胞イメージング-a new horizon-骨のメカニズムをどこまで観ることができるか 蛍光細胞イメージングと骨形態計測で見る骨代謝におけるケモカインシグナルの役割
  李 智媛; 飯村 忠浩
  Journal of Oral Biosciences Supplement, Sep. 2015, Japanese
• 生体機能の多次元バイオイメージング ライブイメージングと数理計測で見る脊椎動物の発生ボディプランの調節機構
  飯村 忠浩
  Journal of Oral Biosciences Supplement, Sep. 2015, Japanese
• 二光子励起顕微鏡を用いた軟骨変性の評価
  清松 悠; 大嶋 佑介; 斎藤 卓; 疋田 温彦; 三浦 裕正; 宮崎 剛; 飯村 忠浩; 今村 健志
  日本整形外科学会雑誌, Sep. 2015, Japanese
• 骨吸収の促進は骨細胞におけるsclerostinの発現を低下させ、骨形成を促進する
  小出 雅則; 小林 泰浩; 山下 照仁; 上原 俊介; 尾崎 友輝; 飯村 忠浩; 中村 美どり; 保田 尚孝; 高橋 直之; 宇田川 信之
  日本骨代謝学会学術集会プログラム抄録集, Jul. 2015, Japanese
• 骨関連細胞ネットワークin vitro再構築系の2光子励起顕微鏡による解析
  疋田 温彦; 飯村 忠浩; 大嶋 佑介; 今村 健志
  日本骨代謝学会学術集会プログラム抄録集, Jul. 2015, Japanese
• マルチスケール画像解析の骨生物医学への応用展開
  飯村忠浩; 李 智媛
  第35回日本骨形態計測学会, 05 Jun. 2015, Japanese
  [Domestic Conference]
• 骨代謝関連細胞ネットワークin vitro再構築系の2光子イメージング
  疋田 温彦; 飯村 忠浩; 大嶋 佑介; 齋藤 卓; 山本 真; 今村 健志
  日本骨形態計測学会雑誌, May 2015, Japanese
• 骨バイオイメージングの新たな展開 マルチスケール画像解析の骨生物医学への応用展開
  飯村 忠浩; 李 智媛
  日本骨形態計測学会雑誌, May 2015, Japanese
• 骨バイオイメージングの新たな展開 第二次高調波発生(SHG)イメージングによる軟骨変性の定量的・組織学的評価法の開発
  大嶋 佑介; 清松 悠; 齋藤 卓; 三浦 裕正; 飯村 忠浩; 今村 健志
  日本骨形態計測学会雑誌, May 2015, Japanese
• 蛍光３次元計測で見る骨細胞の機能的ヘテロジェニティ
  飯村 忠浩
  第7回骨・軟骨フロンティア, 30 Nov. 2014, Japanese
  [Invited], [Domestic Conference]
• DMP1は骨細胞におけるFGF23産生の抑制因子である 蛍光3次元形態計測と細胞生物学的アプローチ
  李 智媛; 山口 朗; 飯村 忠浩
  日本骨代謝学会学術集会プログラム抄録集, Jul. 2014, Japanese
• 骨代謝研究領域におけるバイオイメージング技術の発展からその応用まで ラマン分光・イメージング技術の開発と骨代謝研究への応用
  大嶋 佑介; 飯村 忠浩; 疋田 温彦; 今村 健志
  日本骨代謝学会学術集会プログラム抄録集, Jul. 2014, Japanese
• 骨代謝のダイナミクスの新たな潮流 ケモカイン受容体CX3CR1の骨代謝における役割
  飯村 忠浩; 山口 朗
  日本骨代謝学会学術集会プログラム抄録集, Jul. 2014, Japanese
• Retrieval of regulatory mode of G1/S transition in vertebrate axial development by quantitative live imaging and mathematical models
  IIMURA Tadahiro
  The 37th Naito Conference, 16 Jun. 2014, English
  [Invited], [International presentation]
• Fluorescence morphometric analysis of mechano-sensing osteocytes in bone
  IIMURA Tadahiro
  International Symposium on Mechanobiology 2014, 22 May 2014, English
  [Invited], [International presentation]
• 中軸骨格形態形成の観察と計算モデル
  齋藤 卓; 今村 健志; 飯村 忠浩
  日本骨形態計測学会雑誌, May 2014, Japanese
• 医歯工連携シンポジウム 画像イメージングと骨形態計測の接点 骨細胞の機能的3次元蛍光計測
  飯村 忠浩
  日本骨形態計測学会雑誌, May 2014, Japanese
• 医歯工連携シンポジウム 画像イメージングと骨形態計測の接点 ラマン分光分析を基盤とした骨質計測技術の開発
  大嶋 佑介; 飯村 忠浩; 疋田 温彦; 今村 健志
  日本骨形態計測学会雑誌, May 2014, Japanese
• 医歯工連携シンポジウム 画像イメージングと骨形態計測の接点 骨・軟骨組織の2光子励起顕微鏡・第2次高調波発生を用いた観察
  疋田 温彦; 大嶋 佑介; 清松 悠; 飯村 忠浩; 今村 健志
  日本骨形態計測学会雑誌, May 2014, Japanese
• 中軸骨格の形態発生の分子基盤 蛍光イメージングで見るボディプランと骨形態疾患
  飯村 忠浩
  松本歯学, Dec. 2013, Japanese
• 椎間板発生における転写因子Pax1の役割の検討
  牧野 祐司; 玉村 禎宏; 金子 和夫; 山口 朗; 飯村 忠浩
  日本整形外科学会雑誌, Aug. 2013, Japanese
• 椎間板軟骨の初期発生におけるPax1の役割
  牧野 祐司; 金子 和夫; 山口 朗; 飯村 忠浩
  日本骨代謝学会学術集会プログラム抄録集, May 2013, Japanese
• 3次元蛍光イメージング法による骨発達の分子メカニズムの探索
  飯村 忠浩
  日本口腔科学会雑誌, Jan. 2013, Japanese
• 蛍光3次元イメージング形態計測によるSclerostinの時空間的発現変化と生後骨発達における役割
  渡辺 高; 山口 朗; 飯村 忠浩
  Journal of Oral Biosciences Supplement, Sep. 2012, Japanese
• 異骨症モデルマウスを用いた骨格発生と病態の時空間的解析
  飯村 忠浩
  Journal of Oral Biosciences Supplement, Sep. 2012, Japanese
• 脊椎肋骨異骨症・脊椎胸郭異骨症の発生病因 Mesp2-nullマウスの解析
  牧野 祐司; 高橋 雄; 玉村 禎宏; 田辺 里枝子; 祓川 摩有; 五関 正江; 根; 菅野 純; 金子 和夫; 山口 朗; 飯村 忠浩
  日本整形外科学会雑誌, Aug. 2012, Japanese
• 口腔癌による骨破壊では間質細胞と癌細胞の両者が産生するRANKLが重要である
  佐藤 潔; 李 智媛; 飯村 忠浩; 保田 尚孝; 伊東 昌子; 小村 健; 山口 朗
  日本骨代謝学会学術集会プログラム抄録集, Jul. 2012, Japanese
• 低分子量Gタンパク質Cdc42は四肢形成における軟骨分化と肢芽指間部の細胞死に必須である
  相澤 怜; 山田 篤; 鈴木 大; 飯村 忠浩; 山本 剛; 山口 朗; 山本 松男; 上條 竜太郎
  日本骨代謝学会学術集会プログラム抄録集, Jul. 2012, Japanese
• Bone Biologyの新機軸 3次元蛍光イメージグと計測で見る骨格の発生異常と骨の発達のメカニズム
  飯村 忠浩; 山口 朗
  日本骨代謝学会学術集会プログラム抄録集, Jul. 2012, Japanese
• ３次元蛍光光イメージングによる骨の発達メカニズムと骨代謝研究
  飯村 忠浩
  第68回日本顕微鏡学会学術講演会, 14 May 2012, Japanese
  [Invited], [Domestic Conference]
• モデルマウスを用いた脊椎肋骨異骨症および脊椎胸郭異骨症の病因解析
  牧野 祐司; 高橋 雄; 玉村 禎宏; 田辺 里枝子; 祓川 摩有; 五関 正江; 根; 波多 賢二; 米田 俊之; 菅野 純; 金子 和夫; 山口 朗; 飯村 忠浩
  日本骨形態計測学会雑誌, May 2012, Japanese
• 蛍光3次元イメージング形態計測によるSclerostinの時空間的発現変化の定量的解析と生後骨発達における役割
  飯村 忠浩; 渡辺 高; 牧野 祐司; 金子 和夫; 天笠 光雄; 山口 朗
  日本骨形態計測学会雑誌, May 2012, Japanese
• 蛍光3次元イメージング形態計測による頭頂骨と長管骨の骨細胞ネットワークの構造の定量的比較
  飯村 忠浩; 山口 朗
  日本骨形態計測学会雑誌, May 2012, Japanese
• 骨芽細胞分化と骨形成における骨芽細胞特異的転写因子の細胞内局在変化 蛍光イメージングによる解析
  渡辺 高; 天笠 光雄; 山口 朗; 飯村 忠浩
  日本口腔科学会雑誌, Jan. 2012, Japanese
• Cdc42は四肢形成における軟骨形成と肢芽指間域のアポトーシスを制御する
  相澤 怜; 山田 篤; 鈴木 大; 塚崎 雅之; 山本 剛; 飯村 忠浩; 山口 朗; 山本 松男; 上條 竜太郎
  Journal of Oral Biosciences, Sep. 2011, Japanese
• Multi-dimensional fluorescence imaging approaches for embryonic development and bone biology
  IIMURA Tadahiro
  8th Meeting of Bone Biology Forum, 20 Aug. 2011, English
  [Invited], [International presentation]
• 脊椎肋骨異骨症(SCDO)2型モデルMesp2(-/-)マウスの椎体における骨・軟骨組織の発生・分化過程の解析 分節時計は椎体と椎間板の規則的空間配置に必須である
  牧野 祐司; 田辺 里枝子; 波多 賢二; 五関 正江; 根; 金子 和夫; 山口 朗; 飯村 忠浩
  日本骨代謝学会学術集会プログラム抄録集, Jul. 2011, Japanese
• 矯正力による歯の移動における骨細胞の役割
  松本 力; 飯村 忠浩; 山口 朗
  日本骨形態計測学会雑誌, May 2011, Japanese
• 定量的in situ蛍光イメージングによる骨芽細胞特異的転写因子の骨組織内での分布と細胞内局在の変化の観察
  渡辺 高; 天笠 光雄; 山口 朗; 飯村 忠浩
  日本骨形態計測学会雑誌, May 2011, Japanese
• 骨の成長と代謝における概日リズムの働き 蛍光イメージングによるアプローチと展望
  中根 綾子; 沼野 利佳; 佐藤 博紀; 高木 裕三; 山口 朗; 飯村 忠浩
  日本骨形態計測学会雑誌, May 2011, Japanese
  骨と軟骨の代謝における様々な機能が24時間の周期性パターンを示す事は、ゲノム研究が盛んになる前の1960年代から報告されてきた。初期の研究は組織学的なアプローチが主であった。その後、ヒトの骨代謝関連ホルモンやマーカーの血清レベルが日内変動を示すことが次々と明らかにされてきた。分子クローニング技術の飛躍的進歩により、1990年代終盤には、いくつもの時計遺伝子の一次構造および機能が解明された。その後、転写因子のフィードバックループモデルがリズム中枢として働いている事が提唱され、哺乳類の時計中枢として知られている視交叉上核のみならず、様々な末梢組織においても概日リズムを刻む機構が働いていることが明らかとなってきた。骨の成長と代謝もまた、他の末梢組織と同様に、24時間周期の分子機構によって調節されている事が報告されている。さらに、ポストゲノム解析として、マイクロアレイによるトランスクリプトーム解析や、発光・蛍光のシグナルを用いたバイオイメージングによる時空間的な解析が、さらなる総合的な時計遺伝子群の機能解明のために開発されてきた。臨床的には、骨粗鬆症のような骨代謝疾患に対してより効果的な治療成績を見込んだ薬物投与の時期の決定など、概日リズムと骨に関する知見は重要となってきている。本稿では、骨の生物医学における24時間周期の機能調節に関するトピックをレビューし、最新の蛍光イメージングによるアプローチが骨の生物医学分野にどのように貢献していくのか、その可能性について展望を試みたい。(著者抄録)
• 骨の形態的解析法の進歩 骨のin vivo蛍光イメージングの現状と展望
  飯村 忠浩; 中根 綾子; 姫野 彰子; 杉山 真由; 山口 朗
  日本骨形態計測学会雑誌, May 2011, Japanese
  骨組織を対象にした"イメージング"研究が話題になりつつある。もっとも硬い組織である骨に最新の光を当てて何がわかってきたのか?本稿では、骨格系の発生や骨組織を対象とした蛍光イメージング観察のトピックスを上げ、「骨のin vivo蛍光イメージング」の現状報告とその展望について議論する。(著者抄録)
• 脊椎動物の発生におけるライブイメージング(Illumination of vertebrate development by fluorescence live imaging)
  飯村 忠浩; 杉山 真由; 牧野 祐司; 中根 綾子; 渡辺 高; 山口 朗
  Cytometry Research, Mar. 2011, English
  個体発生は細胞の増殖や移動、機能分化が動的に協調して変遷していく過程である。分子生物学やゲノム科学の進展によって、脊椎動物の発生に関わる様々な共通ルールが明らかとなってきた。蛍光ライブイメージングは、4次元での定量的な解析を可能にし、分子から細胞、組織、器官、個体までのより包括的な理解を助ける。これによって、発生・発達生物医学は、分子の階層的記述から、より動的に理解することが可能になってきた。本稿では、脊椎動物の発生・発達生物医学における最近のトピックをいくつか挙げ、その動向と今後の展望について議論したい。(著者抄録)
• 骨組織の細胞機能イメージング法による新規の機能解析
  飯村 忠浩
  Journal of Oral Biosciences, Sep. 2010, Japanese
• Hox遺伝子群と分節時計による脊椎動物の発生基盤
  飯村 忠浩
  4回北海道大学若手研究者交流会, 30 Jul. 2010, Japanese
  [Invited], [Domestic Conference]
• 骨代謝におけるケモカイン受容体CCR1の機能解析
  星野 昭芳; 飯村 忠浩; 山本 健二; 山口 朗
  日本骨代謝学会学術集会プログラム抄録集, Jul. 2010, Japanese
• 骨イメージングの新展開 超微細構造から細胞機能まで 蛍光イメージングと計測による細胞機能の探索
  飯村 忠浩
  日本骨代謝学会学術集会プログラム抄録集, Jul. 2010, Japanese
• 生体イメージングの現状と応用 脊椎動物の発生におけるライブイメージング
  飯村 忠浩
  Cytometry Research, Jun. 2010, Japanese
• 骨成長における概日リズムの可視化
  中根 綾子; 姫野 彰子; 沼野 利佳; 高木 裕三; 山口 朗; 飯村 忠浩
  日本骨形態計測学会雑誌, May 2010, Japanese
• 蛍光イメージングによる骨細胞の形態・機能分化の計測
  姫野 彰子; 中根 綾子; 和泉 雄一; 山口 朗; 飯村 忠浩
  日本骨形態計測学会雑誌, May 2010, Japanese
• 顎変形症の発症原因解明の最前線 発生遺伝学から脊椎骨異形成症の責任遺伝子の同定へのプロセス
  飯村 忠浩
  日本顎変形症学会雑誌, May 2010, Japanese
• 骨のかたちと機能の進化
  飯村 忠浩
  第16回NPO法人 東京血管疾患研究所セミナー, 12 Dec. 2009, Japanese
  [Invited], [Domestic Conference]
• Real time fluorescence imaging in early embryonic body formation
  IIMURA Tadahiro
  The 26th Naito Conference, 11 Oct. 2009, English
  [Invited], [International presentation]
• 蛍光イメージングで探る脊椎動物発生の基盤システム
  飯村 忠浩
  第1回細胞機能可視化研究会, 09 Oct. 2009, Japanese
  [Invited], [Domestic Conference]
• 蛍光イメージングによる骨発生機構の解明
  飯村 忠浩
  第3回瀬戸内フォーラム, 09 Aug. 2009, Japanese
  [Invited], [Domestic Conference]
• 骨の形態的解析法の進歩 骨のin vivo蛍光イメージングの現状と展望
  飯村 忠浩
  日本骨形態計測学会雑誌, May 2009, Japanese
• Embryonic implications of Hox and the segmentation clock genes for skeletal morphogenesis
  飯村 忠浩
  第3回 Bone Research Seminar, 27 Feb. 2009, Japanese
  [Invited], [Domestic Conference]
• Morphogenetic implications of the segmentation clock and Hox genes in early embryonic spine formation
  IIMURA Tadahiro
  Tokyo Medical and Dental University Global COE Opening Symposium, 06 Sep. 2008, English
  [Invited], [International presentation]
• Regulation and function of Hox genes during chick paraxial mesoderm development
  IIMURA Tadahiro
  RIKEN CDB セミナー, 22 Jul. 2008, Japanese
  [Domestic Conference]
• Collinear activation of Hoxb genes during gastrulation controls the timing of mesoderm formation, The 39th Developmemtal Biology Meeting
  Iimura T; Pourquie O
  The 39th Developmemtal Biology Meeting, Symposium 1:Vertebrate axis formation: Translation of temporal and spatial information, 01 Jun. 2006, English
  [Invited], [Domestic Conference]
• Hox遺伝子群による中胚葉形成タイミングの制御
  飯村 忠浩
  文部科学省科学研究費補助金「特定領域研究」細胞の運命と挙動を支配する細胞外環境のダイナミズム 第1回公開シンポジウム, 06 Mar. 2006, Japanese
  [Invited], [Domestic Conference]
• A two-step mechanism is involved in the collinear positioning of Hox gene expression boundaries along the vertebrate skeletal axis
  Iimura T; Millet S; Malapert P; Dubrulle J; Pourquié O
  44th Annual Midwest Regional Developmental Biology Meeting and Singer Symposium, 06 Jun. 2004, English
  [Domestic Conference]
• 哺乳類顎顔面の発生におけるBMP-Msx遺伝子カスケードの役割
  飯村 忠浩
  解剖学雑誌, Oct. 1999, Japanese
• BMPs as a cartilage inducer during mammalian face development.
  Iimura T; Imai H; Ishiguro K; Ikeda Y; Eto E
  77th General Session & Exhibition of the IADR, 08 Mar. 1999, English
  [Domestic Conference]

• 基本技法, 2024年, 博士後期課程, 歯学院
• 発表・論文執筆法演習Ⅰ, 2024年, 博士後期課程, 歯学院
• 発表・論文執筆法演習Ⅱ, 2024年, 博士後期課程, 歯学院
• 発表・論文執筆法演習Ⅲ, 2024年, 博士後期課程, 歯学院
• 細胞分子薬理学, 2024年, 博士後期課程, 歯学院
• 麻酔薬生体応答学研究, 2024年, 博士後期課程, 歯学院
• 歯学研究概論, 2024年, 博士後期課程, 歯学院
• 細胞内情報伝達解析学研究, 2024年, 博士後期課程, 歯学院
• 歯科学概論Ⅱ, 2024年, 学士課程, 歯学部
• 歯科学概論Ⅰ, 2024年, 学士課程, 歯学部
• 薬理学・歯科薬理学Ⅰ, 2024年, 学士課程, 歯学部
• 健康と社会, 2024年, 学士課程, 全学教育
• 一般教育演習(ﾌﾚｯｼｭﾏﾝｾﾐﾅｰ), 2024年, 学士課程, 全学教育
• 関連臨床医学, 2024年, 学士課程, 歯学部
• 健康と社会, 2024年, 学士課程, 全学教育
• 全人教育演習, 2024年, 学士課程, 歯学部
• アクティブラーニング科目Ⅳ, 2024年, 学士課程, 歯学部
• 薬理学・歯科薬理学Ⅱ, 2024年, 学士課程, 歯学部
• 薬理学・歯科薬理学実習, 2024年, 学士課程, 歯学部

• 2019
  THE JAPANESE ASSOCIATION OF ANATOMISTS
• 2019
  JAPANESE SOCIETY OF DEVELOPMENTAL BIOLOGISTS
• 日仏生物学会
• 口腔医科学フロンティア研究会
• JAPANESE STOMATOLOGICAL SOCIETY
• THE JAPANESE PHARMACOLOGICAL SOCIETY
• THE JAPANESE SOCIETY OF MICROSCOPY
• JAPANESE SOCIETY FOR BONE MORPHOMETRY
• JAPANESE ASSOCIATION FOR ORAL BIOLOGY
• JAPANESE SOCIETY FOR BONE AND MINERAL RESEARCH

• 副甲状腺-交感神経・副腎髄質系連関による新規骨代謝および神経調節機構の解明
  科学研究費助成事業
  27 Jun. 2025 - 31 Mar. 2027
  飯村 忠浩; 木村 徳子; 沼野 利佳; 渡辺 陽久; 沼端 麻里絵
  日本学術振興会, 挑戦的研究(萌芽), 北海道大学, 25K22680
• Pathophysiological investigation of bone-degenerative diseases by the neuroskeletal mapping method
  科学研究費助成事業
  Sep. 2024 - Mar. 2027
  飯村 忠浩
  日本学術振興会, 国際共同研究加速基金(海外連携研究), 北海道大学, 24KK0165
• 骨微小循環改善効果に着目した顎骨壊死の病態解明と育薬研究
  国際医療医療研究開発費
  Apr. 2024 - Mar. 2027
  研究代表者:丸岡豊、研究分担者:飯村忠浩
  国立国際医療研究センター, Coinvestigator, Competitive research funding
• Pharmacological study of a shared genetic risk factor for osteoporosis and Alzheimer's disease
  Grants-in-Aid for Scientific Research
  Apr. 2023 - Mar. 2026
  研究代表者; 李智媛; 研究分担者; 飯村忠浩
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 23K09116
• A drug development study for radiation osteonecrosis of jaw with improving micro vascular-skeletal structure
  Grants-in-Aid for Scientific Research
  30 Jun. 2023 - 31 Mar. 2025
  飯村 忠浩; 丸岡 豊; 樋田 京子; 李 智媛
  Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Exploratory), Hokkaido University, 23K18347
• 第43回日本骨形態計測学会
  2023年度（第29回）助成
  Apr. 2023 - Mar. 2024
  代表者; 飯村忠浩
  （公財）伊藤医薬学術交流財団, Principal investigator, Competitive research funding
• 人体に安全な顔料を用いた医療機器認証マーカーの実用化、および適応拡大に関する研究
  国際医療研究開発費（重点研究）
  Apr. 2023 - Mar. 2024
  研究代表者; 丸岡豊; 研究分担者; 飯村忠浩
  国立国際医療研究センター, Coinvestigator, Competitive research funding
• 非侵襲イメージングによる間葉系幹細胞 品質の自動評価・選択技術の開発
  産学連携創出（スタートアップ研究）補助金
  Apr. 2022 - Mar. 2023
  研究代表者; 飯村忠浩
  ノーステック財団, Principal investigator, Competitive research funding
• 進行性下顎頭吸収・骨代謝連関におけるCCL5の病態および臨床医 学的意義の解明
  国際医療研究開発費
  Apr. 2020 - Mar. 2023
  研究代表者:丸岡豊、研究分担者:飯村忠浩
  国立国際医療研究センター, Coinvestigator, Competitive research funding
• Regulation of tooth movement-initiated mechanotransduction via osteocytic bone remodeling
  Grants-in-Aid for Scientific Research
  01 Apr. 2017 - 31 Mar. 2021
  ISHIHARA Yoshihito
  Orthodontic tooth movement (OTM) is achieved by the remodeling of the periodontal ligament (PDL) and alveolar bone in response to balanced mechanical loading. We investigated the regulatory dynamics of the SOST/Scl expression generated by orthodontic tooth movement (OTM) in vivo and in vitro. Our results provide evidence to support that factors secreted by the PDL, including SOST/Sclerostin, control alveolar bone remodeling through osteocytic SOST/Sclerostin in OTM. In addition, our findings suggest that augmented mechanical stress-mediated Ca2+ oscillations in PDL enhance the production and release of bone regulatory signals.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Okayama University, 17H04413
• 神経・骨連関による頭蓋顎顔面領域の成熟機能骨・再生機構の解明
  基盤研究（B）
  Apr. 2018 - Mar. 2021
  飯村 忠浩
  科学研究費補助金, Principal investigator, Competitive research funding
• 口腔顔面骨格痛シグナルのトランス・オミクス探索と創薬分子情報基盤の構築
  挑戦的研究（萌芽）
  Apr. 2018 - Mar. 2020
  飯村 忠浩
  科学研究費補助金, Principal investigator, Competitive research funding
• Analysis of new therapeutic target molecules for osteoporosis based on the chromatin information
  Grants-in-Aid for Scientific Research
  30 Jun. 2017 - 31 Mar. 2019
  Imai Yuuki; Yanagihara Yuta; Iimura Tadahiro; Saeki Noritaka; Lee Jiwon
  Zinc finger and SCAN domain containing 10 (Zscan10) was identified as a novel transcription factor that is involved in osteoclast differentiation in our previous report. However, the biological functions of Zscan10 are not fully understood. First, Zscan10 KO RAW264 (KO) cells were established by genome editing using CRISPR/Cas9 and single cell sorting. Zscan10 might regulate transcription of the genes that negatively control osteoclastogenesis. To understand gene expression profiles controlled by Zscan10, RNA-seq was performed and stringent analyses identified the haptoglobin gene (Hp) as a possible target of Zscan10. rHp treatment suppressed TRAP activity of KO cells without affecting cell viability. Furthermore, it has been reported that Hp KO mice exhibit decreased bone mass and increased osteoclast number. Taken together, this study suggests that Zscan10 negatively regulates osteoclast differentiation through transcription of Hp.
  Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Exploratory), Ehime University, 17K19728
• Morphometrical and quantitative transcriptome analyses of C-C chemokine receptor 5 in functional structure of osteoclasts
  Grants-in-Aid for Scientific Research
  01 Apr. 2016 - 31 Mar. 2019
  LEE JIWON; Iimura Tadahiro
  This study demonstrated that the blockade of CCR5 using its specific antibodies impaired in vitro human osteoclastogenesis with disorganized actin rings, but not osteoblastogenesis. Ccr5-deficient mice with dysfunctional osteoclasts were resistant to osteoporotic stimulation via the administration of receptor activator of nuclear factor kappa-B ligand (RANKL), which induces osteoporosis independently of the inflammatory and immunomodulatory responses. Furthermore, CCL5, a ligand for CCR5, enhanced the integrin- and chemokine-mediated pathways in osteoclast. The present study experimentally provides further evidence that CCR5 plays an essential role in bone destructive diseases through the functional regulation of osteoclasts, thus suggesting a skeletal benefit of the CCR5-targeting therapy.
  Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Ehime University, 16K20412
• イメージング・シミュレーション・オミクスによる骨格形成機構の多角的解析
  基盤研究（B）
  Apr. 2014 - Mar. 2018
  飯村 忠浩
  科学研究費補助金, Principal investigator, Competitive research funding
• Evolutional investigation of the hematopoietic niches in bone marrow
  Grants-in-Aid for Scientific Research
  01 Apr. 2014 - 31 Mar. 2016
  Yamaguchi Akira; IIMURA Tadahiro; SAKAMOTO Kei
  Hematopoietic organs differed among aquatic and land animals. In mammals, there are two niches to maintain hematopoiesis in bone marrow, osteoblastic niche on bone surface and vascular niche around sinusoid vessels in bone marrow. We found that hematopoiesis is localized on the only bone surface in xenopus, suggesting that only osteoblast niche is developed in xenopus. These results prompted us to explore development of osteoblastic niche during vertebrate evolution. In the present study, we revealed the following findings. 1) Hematopoietic cell localized on only the bone surface of bone marrow. 2) During bone regeneration, hematopoiesis also occurred newly formed bone surface. 3) There are no definite Harvasian canals in cortical bone, indication blood vessels communication between inside and outside of bone marrow i.e. very poor in Xenopus. 4) We are now investingation the changes in distribution of hematopoietic cells on bone surface after inject of 5=FU in Xenopus.
  Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, 26670657
• Comprehensive analyses of the mechanism of bone destruction by oral cancer
  Grants-in-Aid for Scientific Research
  01 Apr. 2013 - 31 Mar. 2016
  Yamaguchi Akira
  We invested the mechanism underling bone destruction by oral cancer, and obtained the
  following results. 1) Fibroblasts locating between cancer cells and bone surface produced IL-6 and its stimulate RANKL synthesis in the fibroblastic cells. 2) Oran cancer cells produced RANKL and it collaborator involved in bone resorption with RANKL synthesized by fibroblasts and osteoblasts. 3) Oral cancer cells produced CXCL2, and it stimulated RANKL production by fibroblastic cells. 4) We succeeded to generate a bone destruction model by transplantation of oral cancer cells into mandibular region of athymic mice.5) Fibroblasts locating between cancer cells and bone surface produced TGF-β and it involved in bone destruction by oral cancers.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), 25253098
• Analysis of the TGF-b signaling in cartilage by nonlinear optics imaging combined with genetic engineering techniques
  Grants-in-Aid for Scientific Research
  01 Apr. 2012 - 31 Mar. 2014
  MIURA Hiromasa; IMAMURA Takeshi; HIKITA Atsuhiko; IIMURA Tadahiro; OSHIMA Yusuke; MIYAZAKI Tsuyoshi
  Multi-photon microscopy enables to characterize and evaluate alternation of collagen matrix in vivo and in vitro based on second harmonic generation (SHG) signal. We performed SHG imaging of the cartilage degeneration induced by enzyme treatment. In the result, changes in SHG signal intensity were observed in the experimental samples. We also established an animal osteoarthritis model by syndesmectomy using H2B-GFP line mice. We successfully evaluated over time the changes in cartilage matrix and chondrocytes by SHG and two-photon excited fluorescence. Furthermore, the articular cartilages of aged mice were observed as the same manner. In comparison with those of young mice, it was possible to evaluate them as spontaneous knee osteoarthritis. Our technique that combined genetic engineering and nonlinear interaction between the molecules and photons to approach the mechanism of cartilage degeneration is promising as a translational research leading to clinical application.
  Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Ehime University, 24659680
• ３次元蛍光イメージングによる骨細胞機能ダイナミズムの可視化と骨の生理・病態解析
  基盤研究（B）
  Apr. 2011 - Mar. 2014
  飯村 忠浩
  科学研究費補助金, Principal investigator, Competitive research funding
• ３次元蛍光イメージングによる骨の形態と機能のヘテロジェナイエティの網羅的可視化
  新学術領域研究 (研究領域提案型)
  Apr. 2011 - Mar. 2013
  飯村 忠浩
  科学研究費補助金, Principal investigator, Competitive research funding
• Investigation for the bone clock development by bioluminescence and fluorescence imaging on brain and bone
  Grants-in-Aid for Scientific Research
  Apr. 2011 - Mar. 2013
  IIMURA Tadahiro; NUMANO Rika; YAMAGUCHI Akira; MIYAWAKI Atsushi
  Bone metabolism is regulated by circadian clock. On the other hand, the central clock system in brain is thought to regulate peripheral clocks including bone. We established a dual imaging system of bioluminescence and fluorescence, which enables us to monitor circadian oscillation from body to cell levels. By exploiting this system, inter-regulation between brain and bone well be further investigated.
  Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Tokyo Medical and Dental University, Principal investigator, Competitive research funding, 23659855
• Serum calcium upregulates Fgf23 expression by Dickopf-1-mediated inhibition of Wnt/beta-catenin signaling in bone
  Grants-in-Aid for Scientific Research
  2011 - 2013
  TAMAMURA Yoshihiro; YAMAGUCHI Akira; IIMURA Tadahiro; SAKAMOTO Kei
  Fgf23 is a phosphaturic factor which is secreted from osteocytes in bone. Fgf23 plays a central role for phosphate metabolism as demonstrated by elevated serum levels of Fgf23 in hypophosphatemic diseases. Post-transcriptional modification of Fgf23 is extensively studied, however mechanisms of transcriptional regulation of Fgf23 are not fully understood. In this study, we demonstrated that serum calcium regulates Fgf23 expression by Dickopf-1-mediated inhibition of Wnt/beta-catenin signaling in bone. This result will provide the possibilities of a new therapy for hypophosphatemic diseases by using anti-Dickpof-1 antibody.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), 23592696
• Maintenance and disturbance of osteonetwork: basic studies on clarification of pathophysiology of craniofacial bone diseases
  Grants-in-Aid for Scientific Research
  2010 - 2012
  YAMAGUCHI Akira; IIMURA Tadahiro; SAKAMOTO Kei; TAMAMURA Sadahiro
  We conducted experiments to clarify the pathophysiology of craniofacial bone diseases based on the concept of osteonetwork, and the following results were published. 1. Development of bioimaging techniques to visualize the netwrork among osteoblasts, osteocytes and osteoclasts. 2. Osteocytes play crucial roles in orthodontic tooth movement. 3.CCN3 is a negative regulator in bone regeneration. $. RANKL plays crucial roles in bone destruction induced by oral squamous cell carcinoma.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Tokyo Medical and Dental University, 22249061
• 脳頭蓋および顎顔面頸部の発生・発達におけ4次元蛍光イメージング法の確立
  挑戦的萌芽研究
  Apr. 2009 - Mar. 2010
  飯村 忠浩
  本研究の目的は、細胞機能を可視化できる蛍光タンパク質プローブを組み込んだ遺伝子導入動物を用いて、3次元空間的および経時的なバイオ・イメージング(4次元蛍光イメージング)を行い、複雑な脳頭蓋および顎顔面頚部の発生・発達過程における細胞機能を可視化し、より統合的に可視化する方法を確立することである。
  本研究代表者らけ、ゼブラ魚由来のCdt1とGemininの細胞周期依存的ユビキチン修飾配列を用いて、蛍光性細胞周期プローブを開発した。さらに、この蛍光プローブを全身に発現する形質転換ゼブラ魚を作製することに成功した。蛍光ライブイメージング法も確立し、脊椎動物の胚発生過程で細胞増殖と分化か協調しながら進行する様相を、初めて生きた動物で観察することに成功した。本研究代表者は、蛍光ライブーメージングによる胚発生過程の観察法の確立の部分で貢献した。この研究成果によって、胚の発生過程で生じる細胞の形態形成運動や分化過程が細胞周期の進行とどのように相関して調節されているのかを、より統合的に探ることが可能になった(Sugiyama M et al., Proc Natl Acad Sci USA.2009)。
  今後は、このような観察技術をもとに、画像解析やシュミレーションによる定性的あるいは定量的なアッセイを進あることで、生物医学的に重要なメカニズムの理解を深めることが重要であると考えられた。
  科学研究費補助金, 挑戦的萌芽研究, 東京医科歯科大学, Principal investigator, Competitive research funding, 21659426
• The better isolation and detection methods of cranial neural crest stem cells (CNCSC) and the therapeutic potentiality of CNCSC in the treatment of craniofacial abnormalities.
  Grants-in-Aid for Scientific Research
  2001 - 2004
  ETO Kazuhiro; OTA Masato; ISEKI Sachiko; IIMURA Tadahiro; IKEDA Masa-aki; NAKAHARA Taka
  We tried isolation and isolation of Cranial Neural Crest Stem Cells for the cell-based therapy in the future indicated as below.
  1)Identification of the stem cell niche of Neural Crest Cells
  a)Distribution pattern of Cranial Neural Crest Cells during Craniofacial morphogenesis in Wnt-1/Cre mouse.
  The distribution pattern of Cranial Neural Crest Cells (CNCCs) was examined in Wnt-1/Cre mouse, which indicate b-galactosidase activity in Neural Crest Cells, during craniofacial morphogenesis. B-galactosidase positive CNCCs were identified in the frontal and facial bone, and these pattern were constructed until E9.5.
  b)Method for identification of Mesenchymal Stem cells in Craniofacial region by chasing the Label-Retaining Cells (LRCs).
  2)Isolation of Cranial Neural Crest Stem Cells (CNCSCs).
  a)Modification the isolation methods of CNCSCs
  3)Molecular understanding of cell proliferation and apoptosis in cranial neural crest cells
  a)Expression pattern of cell-cycle regulators in developing cranial suture
  b)Cell cycle regulation in terminal-differentiated cells in
  c)Twist-dependent regulation of cell proliferation and apoptosis
  We examined Twist function in CNCCs in Twist null mice and found that Twist is essential for the Cranial nerve patterning, cell growth, and anti-apoptotic activity in CNCCs. We also developed the knockdown methods of Twist activity in vitro by DNazyme and, antisense morpholino nucleotide methods.
  d)The transcriptional regulation of cell-cycle regulator E2F1.
  e)Cell-cycle related acetylation of the p220/NPAT by CBP histone acetyltransferase
  4)Clinical potentialities of Cranial Neural Crest Cells
  a)Periodontal tissue regeneration by in situ tissue engineering by the combination of growth factors and scaffolds
  b)Periodontal tissue regeneration by in situ tissue engineering by the combination of cell and scaffolds
  We examined the clinical potentialities of cranial neural crest cells by tissue engineering methods, and data suggested that they will be a powerful tool in cell-based therapy in the future.
  5)Gene expression profiling of the Cranial Neural Crest Stem Cells
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Tokyo Medical and Dental University, 13357015
• Characterization of factors controlling the differentiation of periodontal ligament cells
  Grants-in-Aid for Scientific Research
  2000 - 2001
  KASUGAI Shohei; OIDA Shinichiro; ODA Shigeru; KURODA Shinji
  The purpose of this research was to characterize molecules which control the differentiation and function of periodontal ligament (PDL) cells. We constructed cDNA library of bovine PDL tissue and cloned cDNA of S100A4, one of the calcium binding proteins. We observed that mRNA expression level of S100A4 in PDL of erupted teeth was high and that PDL cells secreted this protein, which localized adjacent to collagen fibers. Addition of recombinant S100A4 protein to oseteoblastic culture inhibited mineralization. In culture, PDL cells under mechanical stress inceased mRNA expression of S100A4 together with cytoskeletal proteins. Although the expression level of this protein in MC3T3-E1 cells (osteoblastic cells) was low, inhibition of S100A4 expression in MC3T3-E1 cells stimulated mineraliztion in culture. These results strongly indicate that S100A4 is an inhibitor of meranlization in PDL tissue. On the other hand, porcine enamel matrix derivative (EMD) stimulated growth and differentiation of bovine PDL cells and Kusa cells (derived from mouse bone marrow), resulting the stimulation of mineralization in these culture. The stimulatory effects on osteoblastic cells could also contribute to periodontal tissue regeneration. It is important to clarify effective molecule (s) in EMD and Kusa cells are useful for this investigation. Porcine recombinant amelogenin stimulated the differentiation of Kusa cells indicating the possibility that amelogenin is the effective molecule.Although further studies are necessary, we could apply recombinant amelogenin to periodontal tissue regeneration.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B)., Tokyo Medical and Dental University, 11470460
• Investigation of organaizers in vertebrate craniofacial development
  Grants-in-Aid for Scientific Research
  1998 - 2001
  ETO Kazuhiro; IIMURA Tadahiro; ISEKI Sachiko; IKEDA Masa-aki; IMAI Hajime; OTA Masato
  Craniofacial development of vertebrates involves several tissue components of early stage embryos, ectoderm, neural tube, cranial neural crest cells, mesodem, foregut endoderm. To investigate the mechanism of craniofacial development provides us with not only understanding of craniofacial anomalies but also basic data for craniofacial reconstitution. Our current research results have suggested that the initiation and development of head organs requires spatio-temporal localization and interaction of these tissues:
  1 ) mandible arch formation requires the interaction between the oral ectoderm and posterior midbrain crest cell-derived mesenchyme at the proximooral part of the first pharyngeal arch;
  2) tooth bud formation begins where oral ectoderm, foregut endoderm and neural crest cells meet;
  3) Rathke's pouch is derived from the ectoderm which locates just anterior to the rostral part of the neural plate and later composes the stomodeal roof with foregut endoderm;
  4) mouse coronal and sagittal sutures are formed at a neural crest-mesoderm interface, and the initiation of skull vault bone osteogenesis coincides with the dynamic growth of cerebral hemispheres caudally. This supports the idea of organizing center that tissue boundary generates positional information for organogenesis, proposed by Meinhardt. Based on these results we are planning to clarify the mechanism of the interaction, key molecules of the events and regulation of their expression.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Tokyo Medical and Dental University, Graduate School, 10307043
• 頭蓋顔面骨の発生分化におけるホメオボックス型転写因子の機能解析
  奨励研究(A)
  Apr. 1997 - Mar. 1999
  飯村 忠浩
  RT-PCR法,cDNA library スクリーニングを行い、ラットBMP-2,BMP-4およびMsx-1,Msx-2の遺伝子をクローニングした。ラット胚(胎生9.5日から12.5日)におけるin situhybrldizationにより、BMP-2.-4.およびMsx-1.-2の発現を解析したところ、顔面の初期発生過程において、BMP-2,BMP-4は時期特異的、領域特異的に発現しその発現領域はMsx-1,Msx-2の発現領域と亀なっていた.ラット12.5日胚の顔面突起において、BMP-2,-4は上皮とその直下の間葉細胞に発現が見られ、Msx-1,Msx-2の発現はBMP発現領域を含む上皮と間葉により広く見られた。ラット12.5日胚の下顎突起の器官培養にBMP-ビーズを埋植させた実験の結果から、BMPによってMsx-1,Msx-2の発現が誘導され、異所性に軟骨組織が誘導された。また同様な器官培養系にAntisense Oligonucleotideを導入しMsx-1,Msx-2の発現を阻害する実験を行ったところ、下顎軟骨に形態異愉が見られた。以上のことがら、頭蓋顔面の発生過程において、BMPの機能は頭部神経堤細胞を軟骨へ分化誘導することでり、さらにMsxのようなホメオポックス遺伝子の発現を調節することにより軟骨の形態形成をも調節していることが明らかとなった。
  科学研究費補助金, 奨励研究(A), 東京医科歯科大学, Principal investigator, Competitive research funding, 09771530
• 骨組織へのバイオターゲッティングに関する研究
  科学研究費助成事業
  1998 - 1998
  春日井 昇平; 宮本 謙一; 飯村 忠浩; 大井田 新一郎; 藤沢 隆一
  骨組織に選択的に薬物を輸送することが可能となれば、骨疾患に対する薬物治療の大きな前進を期待することができる。ハイドロキシアパタイト(HA)は硬組織(骨・歯)以外の組織には存在しないことから、薬物にHA選択性を持たせることにより骨組織に薬物を選択的に輸送することが可能となる。そこで、酸性アミノ酸が繰り返し配列した小ペプチドを、骨組織への薬物の選択的輸送法のキャリアーとして用いる可能性について検討した。Aspの6回繰り返し配列(Asp)_6にfluorescein(FITC)を結合させた(Asp)_6-(FITC)を作製し、HAに対する結合試験おこなった。(Asp)_6-FITCは全身投与すると骨組織に沈着することが知られているテトラサイクリン・カルセインと同程度にHAに結合したが、FITCはHAに結合しなかった。(Asp)_6-FITCをラットに投与し血中濃度を測定したところ、(Asp)_6-FITCの血中濃度の急速な減少が観察された。(Asp)_6-FITCを投与したラットの骨および歯の研摩標本を作製し、共焦点レーザー顕微鏡で観察したところ、骨および歯に明瞭な蛍光標識線が見られたが、その他の組織においては蛍光が観察されなかった。一方、FITCを投与したラットにおいてはどの組織においても蛍光は観察されなかった。(Asp)_6-FITCを投与すると、24時間後にFITCの約95%尿中に排泄され約2%が骨に蓄積した。(AsP)_6-FITCを投与したマウスの大腿骨において、骨に蓄積したFITCの生物学的半減期は約14日であった。骨組織に作用を示す種々の薬物を(Asp)_6により修飾したところ、これらの薬物のHA親和性が増大した。これらの結果より(Asp)_6は骨組織への選択的薬物輸送法のためのキャリアーとして有効であることが示唆された。今後は、骨減少症の動物モデルを用いて、(Asp)_6により修飾した薬物の治療効果について検討する予定である。
  日本学術振興会, 特定領域研究(A), 東京医科歯科大学, 10145208
• Selective Drug Delivery to Bone
  Grants-in-Aid for Scientific Research
  1997 - 1998
  KASUGAI Shohei; MIYAMOTO Ken-ichi; IIMURA Tadahiro; OIDA Shinichiro; FUJISAWA Ryuichi
  Targeting a drug on hydroxyapatite (HA) could be a promising way for selective drug delivery to bone because HA does not exists in soft tissues. Some of non-collagenous proteins in bone have repeating sequence of acidic amino acids in their structures as possible HA-binding sites. The purpose of this study is. to examine whether a small peptide of repetitive Asp could work as a carrier for selective drug delivery to bone. Fluorescein (Flu) were conjugated with (Asp)_6 peptide and affinities of this compound and bone-seeking compounds (tetracycline, calcein, alizarin red S and tiludronate) to HA were spectrophotometrically measured after incubation with HA bead solution and centrifugation. Flu or (Asp)_6-Flu (1mg or 3mg, respectively, in 200mu was intravenously injected and fluorescent intensity in blood plasma was periodically measured. Twenty-four hours after injection, ground sections of bones and teeth and cryosections of soft tissues were prepared and then examined under confocal laser scanning microscope. Flu did not bind to HA, however, (Asp)_6-Flu showed high affinities to HA, which were comparable to the bone-seeking compounds. Fluorescence measurement in the plasma revealed rapid excretion of both Flu and (Asp)_6-Flu from the blood. Biological half lives of Flu and (Asp)_6-Flu were 39 and 60 minutes, respectively. In the rats injected with (Asp)_6-Flu systemically, clear fluorescent lines were observed in bones and teeth whereas no fluorescence was detected in soft tissues (brain, muscle, kidney, liver, spleen, thymus, heart, intestine, dermal connective tissue). In the animals injected with Flu systemically, the fluorescence was detected in neither haul nor connective tissues. We also examined biological half life of Flu in femurs after injecting (Asp)_6-Flu into mice and it was 14 days. Furthermore, we conjugated estradiol with (Asp)_6 and (Asp)_6-estradiol showed high affinity to HA whereas estradiol did not. (Asp)_6-estradiol inhibited bone loss in ovariectomized mice without increasing uterine weight. These results indicate that (Asp)6 conjugation increases drug affinity to HA and could be effective as a carrier for drug delivery to bone.
  In this research project we also demonstrated that rolipram, a phosphodiesterase 4 (PDE4) inhibitor which inhibits cAMP degradation specifically, exerts anabolic effect in bone and that XT-44, which is a new PDE4 inhibitor developed by Dr. Miyamoto, one of the investigators in this project, is therapeutically effective in three osteopenia models : carcinoma bearing rats, ovariectomized rats and nurolectomized rats. Furthermore we clarified active site of fibroblast growth factor (FGF) 4, and produced a recombinant protein of N-terminal truncated FGF4 containing its active site. Systemic administration with this short FGF4 to normal mice increased femur BMD.PDE4 inhibitors and this short FGF4 could be new candidates for therapeutic drugs of osteopenia, however systemic administration of these molecules might also exert unfavorable effects in other tissues. Targeting of these molecules to bone by conjugation with (Asp) 6 could open a new avenue for treatment of osteopenia including osteoporosis.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Tokyo Medical and Dental University, 09557163
• The establishment of a new type of long-term culture system in which the whole embryo cultu lowed by a mandibular organ culture.
  Grants-in-Aid for Scientific Research
  1996 - 1998
  ETO Kazuhiro; IMAI Hjime; ISEKI Sachiko; IIMURA Tadahiro; IKEDA Masaaki
  1. Teeth are formed by reciprocal interactions between the epithelium and mesenchyme in the first pharyng. Although the contribution of midbrain and hindbrain crest cells to the first pharyngeal arch has been previouined in rodent embryos no direct evidence exists that these cells are actually involved in the dental mesenc order to elucidate the contribution of the cranial neural crest cells in the tooth formation, we first identified th tion sites and stages poviding the crest, cells that, migrate to the presumed tooth-forming region of the m.prominence. Focal labeling with DiI was performed at the midbrain and anterior hindbrain crests in rat embryo labeled embryos were cultured for 30 or 60 hr. The resultant migration pattern indicated that posterior midb cells emigrating by the end of the 4-somite stage predominantly migrated to the region where tooth buds develop. Second, we established a new type of long-term culture system in which the whole embryo culture is by a mandibular organ culture. Using this system, rat embryos were maintained from the early somite stag molars in the explants were able to reach the bud stage within 8 days. Finally, to ascertain if posterior midb cells emigrating by the end of the 4-somite stage were involved in the dental mesenchyme, these cells were lat DiI and processed the long-term culture. Labeled crest cells were found to be clearly detectable in t mesenchyme. These findings indicate that the early-emigrating posterior midbrain crest cells ontribute to m molar tooth development in rat embryos.
  2. Classical transplantation experiments with various amphibian tissues have shown that tooth development re* only oral ectoderm and neural crest but also foregut endoderm. In addition, histological observation of oral n showed that the tooth germs are initiated in some ectodermal cells and neural, crest cells adjacent to foregut e These studies suggest that tooth initiation requires the presence and cooperation of these three components. mals, however, there is no direct evidence that tooth formation is involved in the region of oral ectoderm a* foregut endoderm. In order to elucidate the contribution of foregut endoderm to tooth formation, we establist type of endodermal cell tracing system with a recombinant adenovirus called Adex-lacZ, and performed en cell tracing in a long term culture system. Cells labelled with Adex-lacZ were seen next to non-labelled th epithelium, presumptive incisor epithelium. These findings show the first direct evidence in mammals that too taks place in the specified part of oral ectoderm adjacent to foregut endoderm, suggesting that foregut plays a role in tooth initiation.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Tokyo Medical and Dental Univercity, 08557097
• 骨形成タンパク質(BMP)のシグナル伝達機構に関する分子生物学的研究
  特別研究員奨励費
  Jul. 1995 - Mar. 1997
  飯村 忠浩
  科学研究費補助金, Principal investigator, Competitive research funding
• Cloning of the Genes Specifically Expressed in Teeth
  Grants-in-Aid for Scientific Research
  1995 - 1996
  KASUGAI Shohei; IIMURA Tadahiro; OIDA Shinichiro
  We constructed cDNA library of bovine periodontal ligament (PDL) and then tried to clone cDNA of the genes specifically expressed in PDL not expressed in calvaria, using the gene subtraction technique, however this experiment was unsuccessful. PDL is a unique tissue because it maintains its function under extent mechanical stress caused by occlusion. We speculate that Ca signaling and cytoskeletal elements in PDL cells play important roles in this uniqueness. Since S100A4 is a Ca-binding protein and interacts with cytoskeletal elements, we focused our research on this protein in PDL.We cloned bovine S100A4 cDNA from PDL cDNA library. The highest level of S100A4 mRNA expression was ovserved among the oral tissues examined. PDL of erupted teeth expressed this gene higher than OPDL of unerupted teeth and application of mechanical stress to cultured PDL cells increased the expression level of this gene, indicating stimulative effect of mechanical stress on S100A4 expression. Immunohistological study demonstrated intercellular and extracellular localization of S100A4 in PDL.Western blotting of the culture medium of PDL cells and analysis of S^<35>-methionine labeled culture of PDL cells demonstrated the existence of S100A4 in the medium. Thus, it is likely that PDL cells secrete S100A4 extracellularly. Osteogenic cells produce mineralized-tissue in culture and addition of recombinant S100A4 protein in this culture system inhibited mineralization. In calvaria development, S100A4 mRNA expression was detected in early stage, however its expression was undetectable when mineralization started. Furthermore, S100A4 expression of osteoblasts was not detected in situ Hybridization experiment. Thus, it is possible that S100A4 is a inhibitory factor for mineralization. We can conclude that mechanical stress stimulates S100A4 expression in PDL cells and PDL cells secrete S100A4, which acts as an inhibitor for mineralization although further study is necessary to prove this story.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Tokyo Medical and Dental University, 08457486
• 骨形成タンパク質(BMP)の細胞内シグナル伝達機構に関する分子生物学的研究
  特別研究員奨励費
  Apr. 1992 - Mar. 1995
  飯村 忠浩
  科学研究費補助金, Principal investigator, Competitive research funding

• 愛媛県立松山東高等学校スーパーグローバルハイスクール事業
  Apr. 2015 - Sep. 2016
  Lecturer