博士（環境科学）, 北海道大学, Mar. 2017

褐藻

走化性

走光性

運動

鞭毛

配偶子

受精

生理

Cell biology

Phycology

Life Science, Cell biology, 藻類学

Flagellar proteomic analysis of the brown alga Mutimo cylindricus revealed a novel calcium-binding protein abundantly localized in the anterior flagellum.
Nana Kinoshita-Terauchi; Kogiku Shiba; Makoto Terauchi; Hideki Noguchi; Kazuo Inaba
Protist, 175, 6, 126070, 126070, Dec. 2024, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
English, Scientific journal, Mutimo cylindricus gametes have two flagella with different structures : an anterior and a posterior flagellum. Their flagellar waveforms are regulated by calcium ions through various mechanisms, however the factors involved in this regulation remain largely unknown To elucidate the molecular basis underlying the difference between the two flagella, we performed a flagellar proteomic analysis of male M. cylindricus gametes. We identified 848 proteins shared with Ectocarpus siliculosus, including 28 calcium-binding proteins. Among the EF-hand proteins, a 111 kDa protein showed predominant localization along the anterior flagellum. Immunogold localization suggested that this protein is associated with outer doublet microtubules. This is the first report to show heterogeneous localization of a calcium-binding protein between two flagellar axonemes and suggests that calcium-binding proteins are involved in the specific regulation of the anterior flagellum.

Distinct regulation of two flagella by calcium during chemotaxis of male gametes in the brown alga Mutimo cylindricus (Cutleriaceae, Tilopteridales).
Nana Kinoshita-Terauchi; Kogiku Shiba; Taiki Umezawa; Kazuo Inaba
Journal of phycology, 60, 2, 409, 417, Apr. 2024, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
English, Scientific journal, Brown algal male gametes show chemotaxis to the sex pheromone that is released from female gametes. The chemotactic behavior of the male gametes is controlled by the changes in the beating of two flagella known as the anterior and posterior flagellum. Our previous study using Mutimo cylindricus showed that the sex pheromone induced an increment in both the deflection angle of the anterior flagellum and sustained unilateral bend of the posterior flagellum, but the mechanisms regulating these two flagellar waveforms were not fully revealed. In this study, we analyzed the changes in swimming path and flagellar waveforms with a high-speed recording system under different calcium conditions. The extracellular Ca2+ concentration at 10-3 M caused an increment in the deflection angle of the anterior flagellum only when ionomycin was absent. No sustained unilateral bend of the posterior flagellum was induced either in the absence or presence of ionomycin in extracellular Ca2+ concentrations below 10-2 M. Real-time Ca2+ imaging revealed that there is a spot near the basal part of anterior flagellum showing higher Ca2+ than in the other parts of the cell. The intensity of the spot slightly decreased when male gametes were treated with the sex pheromone. These results suggest that Ca2+-dependent changes in the anterior and posterior flagellum are regulated by distinct mechanisms and that the increase in the anterior flagellar deflection angle and sustained unilateral bend of the posterior flagellum may not be primarily induced by the Ca2+ concentration.

Enantioselective Total Synthesis of Multifidene, a Sex Pheromone of Brown Algae
Taiki Umezawa; Misaki Hara; Nana Kinoshita-Terauchi; Fuyuhiko Matsuda
Organics, 3, 3, 187, 195, MDPI AG, 08 Jul. 2022, [Peer-reviewed]
English, Scientific journal, The total synthesis of multifidene, a sex pheromone found in brown algae, is described. The synthesis features the highly enantioselective and diastereoselective addition reaction of an aldehyde to a nitroolefin in the presence of a Hayashi–Jørgensen catalyst and a Nef reaction initiated by visible light irradiation. These key reactions enabled the 11-step synthesis from commercially available compounds. The synthetic pheromones are examined with gametes.

Flagellar motility during sperm chemotaxis and phototaxis in fucalean algae
Nana Kinoshita-Terauchi; Alecia Bellgrove; Kogiku Shiba; Kazuo Inaba
European Journal of phycology, 56, 1, 85, 93, Jun. 2020, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal, 12432734

High potassium seawater inhibits ascidian sperm chemotaxis, but does not affect the male gamete chemotaxis of a brown alga.
Nana Kinoshita-Terauchi; Kogiku Shiba; Makoto Terauchi; Francisco Romero; Héctor Vincente Ramírez-Gómez; Manabu Yoshida; Taizo Motomura; Hiroshi Kawai; Takuya Nishigaki
Zygote (Cambridge, England), 27, 4, 225, 231, Aug. 2019, [Peer-reviewed], [Lead author], [International Magazine]
English, Male gamete chemotaxis towards the female gamete is a general strategy to facilitate the sexual reproduction in many marine eukaryotes. Biochemical studies of chemoattractants for male gametes of brown algae have advanced in the 1970s and 1980s, but the molecular mechanism of male gamete responses to the attractants remains elusive. In sea urchin, a K+ channel called the tetraKCNG channel plays a fundamental role in sperm chemotaxis and inhibition of K+ efflux through this channel by high K+ seawater blocks almost all cell responses to the chemoattractant. This signalling mechanism could be conserved in marine invertebrates as tetraKCNG channels are conserved in the marine invertebrates that exhibit sperm chemotaxis. We confirmed that high K+ seawater also inhibited sperm chemotaxis in ascidian, Ciona intestinalis (robusta), in this study. Conversely, the male gamete chemotaxis towards the female gamete of a brown alga, Mutimo cylindricus, was preserved even in high K+ seawater. This result indicates that none of the K+ channels is essential for male gamete chemotaxis in the brown alga, suggesting that the signalling mechanism for chemotaxis in this brown alga is quite different from that of marine invertebrates. Correlated to this result, we revealed that the channels previously proposed as homologues of tetraKCNG in brown algae have a distinct domain composition from that of the tetraKCNG. Namely, one of them possesses two repeats of the six transmembrane segments (diKCNG) instead of four. The structural analysis suggests that diKCNG is a cyclic nucleotide-modulated and/or voltage-gated K+ channel.

A brown algal sex pheromone reverses the sign of phototaxis by cAMP/Ca2+ dependent signaling in brown algal male gametes of Mutimo cylindricus (Cutleriaceae)
Nana Kinoshita-Terauchi; Kogiku Shiba; Taiki Umezawa; Fuyuhiko Matsuda; Taizo Motomura; Kazuo Inaba
Journal of photochemistry and photobiology B, 192, 113, 123, Mar. 2019, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal, 12432734

褐藻の有性生殖における複合的走性システムの解明
寺内菜々
化学と生物, 57, 549, 553, 2019, [Peer-reviewed], [Invited], [Lead author, Last author, Corresponding author]
Japanese, Research institution, 12432734

褐藻遊走細胞の走化性・走光性研究の新展開
木ノ下菜々
藻類 Jpn. J. Phycol., 66, 27, 31, 2018, [Peer-reviewed], [Invited], [Lead author, Last author, Corresponding author]
Japanese, Scientific journal

Calcium Control of the Sign of Phototaxis in Brown Algal Gametes of Mutimo cylindricus
Nana Kinoshita; Chikako Nagasato; Taizo Motomura
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 93, 5, 1216, 1223, Oct. 2017, [Peer-reviewed], [Lead author]
English, Scientific journal

Phototaxis and chemotaxis of brown algal swarmers
Nana Kinoshita; Chikako Nagasato; Taizo Motomura
JOURNAL OF PLANT RESEARCH, 130, 3, 443, 453, May 2017, [Peer-reviewed], [Invited], [Lead author]
English, Scientific journal

Chemotaxis and phototaxis of gametes in brown algae having two heterogeneous flagella
Nana Terauchi
Hokkaido University, 2017, [Peer-reviewed]
English, Doctoral thesis

Chemotactic movement in sperm of the oogamous brown algae, Saccharina japonica and Fucus distichus
Nana Kinoshita; Chikako Nagasato; Taizo Motomura
PROTOPLASMA, 254, 1, 547, 555, Jan. 2017, [Peer-reviewed], [Lead author]
English, Scientific journal

Flagellar waveforms of gametes in the brown alga Ectocarpus siliculosus
Nana Kinoshita; Kogiku Shiba; Kazuo Inaba; Gang Fu; Chikako Nagasato; Taizo Motomura
EUROPEAN JOURNAL OF PHYCOLOGY, 51, 2, 139, 148, May 2016, [Peer-reviewed], [Lead author]
English, Scientific journal

褐藻の受精〜性フェロモンと鞭毛運動〜
木ノ下菜々; 本村泰三
JAMBIO News Letter, 7, 6, 2016, [Invited], [Lead author]
Japanese, Research society

Chemotaxis in the anisogamous brown alga Mutimo cylindricus (Cutleriaceae, Tilopteridales)
Nana Kinoshita; Chikako Nagasato; Atsuko Tanaka; Taizo Motomura
PHYCOLOGIA, 55, 4, 359, 364, 2016, [Peer-reviewed], [Lead author]
English, Scientific journal

Three-dimensional organization of flagellar basal apparatus in Ectocarpus gametes
Nana Kinoshita; Gang Fu; Toshiaki Ito; Taizo Motomura
PHYCOLOGICAL RESEARCH, 64, 1, 19, 25, Jan. 2016, [Peer-reviewed], [Lead author]
English, Scientific journal

沖縄島西方沖から採集された海藻（Ⅱ）
大葉英雄; 木ノ下菜々; 板木拓也
独立行政法人産業技術総合研究所 (地質調査総合センター), 55, 205, 211, 2011, [Peer-reviewed]
Japanese, Research institution

最新精子学
寺内菜々, 藻類の精子 25章
筑波大学出版会, Dec. 2025, [Contributor]

Fertilization of brown algae: flagellar function in phototaxis and chemotaxis. Sexual reproduction in Animals and Plants
Gang Fu; Nana Kinoshita; Nagasato Chikako; Taizo Motomura
Springer, 2014, [Joint work]

褐藻の有性生殖において複合的に働く 走化性・走光性のメカニズムの解明
寺内菜々
北海道植物学会, 09 Dec. 2025, Japanese, Invited oral presentation
09 Dec. 2025 - 09 Dec. 2025, [Invited]

Distinct regulation of two flagella of gametes in brown alga Mutimo cylindricus
Nana Kinoshita-Terauchi; Kogiku Shiba; Makoto Terauchi; Kazuo Inaba
APPF, 15 Apr. 2024, English, Poster presentation
14 Apr. 2024 - 18 Apr. 2024, 37035734

褐藻雄性配偶子でみられる走化性・走光性のシグナリング機構
寺内菜々
ジオラマ若手勉強会, 07 Oct. 2023, Japanese, Invited oral presentation
07 Oct. 2023 - 07 Oct. 2023, [Invited]

褐藻雄性配偶子のシグナリング機構の解明～性フェロモンによる走化性と走光性～
寺内菜々
第７回JAMBIO フォーラム, 10 Mar. 2022, Invited oral presentation
[Invited]

性フェロモンによる褐藻雄性配偶子の走光性符号の転換
寺内菜々
化学生態研究会, Jun. 2018, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Effect of sex pheromone on the sign of phototaxis in brown algal male gametes
Nana Kinoshita; Kogiku Shiba; Kazuo Inaba; Taiki Umezawa; Taizo Motomura
11th International Phycological Congress, Aug. 2017, English, Poster presentation
[International presentation]

不等長2本鞭毛を有する褐藻配偶子の正負走光性の転 換とカルシウムイオンの関係
木ノ下菜々; 長里千香子; 本村泰三
日本植物学会第80回沖縄大会, Sep. 2016, Japanese, Poster presentation
[Domestic Conference]

褐藻における雄性配偶子の走化性～同形・異形配偶子接合と卵生殖の比較解析～
木ノ下菜々; 長里千香子; 本村泰三
日本藻類学会第40回大会東京大会, Mar. 2016, Japanese, Oral presentation
[Domestic Conference]

褐藻ムチモ (異形配偶子接合) の走化性における鞭毛運動解析
木ノ下菜々; 長里千香子; 本村泰三
日本植物学会第79回大会新潟, Sep. 2015, Japanese, Poster presentation
[Domestic Conference]

褐藻シオミドロ配偶子における走化性について
木ノ下菜々; 長里千香子; 本村泰三
日本藻類学会第39回大会福岡大会, 2015, Japanese, Oral presentation
[Domestic Conference]

褐藻遊泳細胞における鞭毛運動の解析
木ノ下菜々
JAMBIOフォーラム, Jan. 2014, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Analysis of flagellar motion in brown algal swarmers
Nana Kinoshita; Fu Gang; Chikako Nagasato; Taizo Motomura
6th international meeting on the model brown alga Ectocarpus, 2014, English, Poster presentation
[International presentation]

褐藻生殖細胞の着底および単為発生メカニズムの解明
科学研究費助成事業
Apr. 2024 - Mar. 2029
日本学術振興会, 若手研究, 筑波大学, Principal investigator, 24K18150

褐藻類の走光性・走化性の系統プロファイリングによる複合的走性の制御因子の探索
Apr. 2025 - Mar. 2026
データサイエンス共同利用基盤施設 ROIS-DS-JOINT, Principal investigator, 036RP2025

褐藻類の走光性・走化性の系統プロファイリングによる複合的走性の制御因子の探索
ROIS-DS-JOINT
Jul. 2024 - Mar. 2025
データサイエンス共同利用基盤施設, 筑波大学, Principal investigator, 041RP2024

褐藻の有性生殖において複合的に働く走化性・走光性の統一的解明
特別研究員奨励金（RPD）
Apr. 2022 - Mar. 2025
日本学術振興会

不等長２本鞭毛が統御する新規因子の解明
特別研究員奨励金（PD）
Apr. 2017 - Mar. 2022
日本学術振興会, Principal investigator, Competitive research funding