Researcher basic information

• PhD (Hokkaido University)

• https://researchmap.jp/mahi2647?lang=en

• https://orcid.org/0000-0001-6635-722X
• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=202201001804869147

• 60830031

• https://orcid.org/0000-0001-6635-722X

• 202201001804869147

• Shortwave-infrared imaging
• Organic synthesis
• Fluorescent probes
• Molecular imaging

• Life Science, Bioorganic chemistry

• Bachelor's degree program, School of Science
• Master's degree program, Graduate School of Life Science
• Doctoral (PhD) degree program, Graduate School of Life Science

Research activity information

• Oct. 2021, Best poster award RIKEN BDR retreat-2021
• Oct. 2018, Japan Student Services Organization (JASSO) scholarship award
• Oct. 2014, Japan government MEXT scholarship award

• Sphingomyelin synthase inhibitors from edible mushrooms prevent overweight and improve vitamin D homeostasis in a mouse model of diet-induced obesity
  Enkhmaa Enkhbat; Yuta Murai; Kohei Yuyama; Hui Sun; Mahadeva M. M. Swamy; Yoshiko Suga; Masaki Anetai; Kenji Monde
  Frontiers in Nutrition, 12, Frontiers Media SA, 10 Dec. 2025
  Scientific journal, Introduction
  
  Sphingomyelin synthase (SMS) is a key enzyme in sphingolipid metabolism that converts phosphatidylcholine and ceramide into sphingomyelin and diacylglycerol, thereby influencing lipid signaling and metabolic regulation. Identifying natural SMS inhibitors is of interest for the prevention and management of obesity and related disorders.
  
  Methods
  
  We screened more than 860 extracts from medicinal plants and mushrooms using a cell-based assay for SMS inhibition. Active fractions were purified, and grifolin and grifolic acid were isolated from the edible mushroom Albatrellus confluens (Alb. & Schwein.) Kotl. & Pouzar and structurally confirmed. Their inhibitory activity was validated in vitro against sphingomyelin synthase 1 (SMS1) and sphingomyelin synthase 2 (SMS2), and their metabolic effects were tested by dietary supplementation (0.1% in high-fat diet) in male C57BL/6J mice for 9 weeks.
  
  Results
  
  Both compounds inhibited SMS activity at low micromolar concentrations. In vivo , they significantly reduced body weight gain, improved glucose tolerance, and alleviated hepatic steatosis in high-fat diet–fed mice. Treatment also preserved circulating 25-hydroxyvitamin D₃ [25(OH)D₃] concentrations and downregulated hepatic expression of the vitamin D–catabolizing enzyme CYP24A1.
  
  Conclusion
  
  These findings demonstrate, for the first time, that mushroom-derived metabolites can act as dual-substrate SMS inhibitors with beneficial effects on lipid and vitamin D metabolism. Grifolin and grifolic acid thus represent promising candidates for development as functional food components or nutraceuticals to prevent obesity and metabolic syndrome.
• Shortwave Infrared (SWIR)-Emitting Tamoxifen-Conjugated Cyanine Dyes for Estrogen Receptor-Targeted Cancer Imaging
  Aravind Kanda Swamy; Mahadeva M. M. Swamy; Kohei Yuyama; Takeshi Jin; Kenji Monde
  ACS Medicinal Chemistry Letters, 16, 8, 1641, 1647, Jul. 2025, [Peer-reviewed]
• Screening of a Fraction with Higher Amyloid β Aggregation Inhibitory Activity from a Library Containing 210 Mushroom Extracts Using a Microliter-Scale High-Throughput Screening System with Quantum Dot Imaging
  Gegentuya Huanood; Mahadeva M. M. Swamy; Rina Sasaki; Keiya Shimamori; Masahiro Kuragano; Enkhmaa Enkhbat; Yoshiko Suga; Masaki Anetai; Kenji Monde; Kiyotaka Tokuraku
  Foods, 13, 23, 3740, 3740, MDPI AG, 22 Nov. 2024, [Peer-reviewed]
  Scientific journal, Alzheimer’s disease (AD) is a highly prevalent neurodegenerative disease hallmarked by amyloid plaques and neurofibrillary tangles. Amyloid plaques are formed by the amyloid β (Aβ) aggregation, so substances that inhibit this aggregation are useful for preventing and treating AD. Mushrooms are widely used medicinal fungi with high edible and nutritional value. Mushrooms have a variety of biologically active ingredients, and studies have shown that they have certain effects in anti-bacterial, anti-oxidation, anti-inflammatory, anti-tumor, and immune regulation. Previously, we developed a microliter-scale high-throughput screening (MSHTS) system using quantum dot (QD) nanoprobes to screen Aβ aggregation inhibitors. In this study, we appraised the Aβ aggregation inhibitory activity of 210 natural mushrooms from Hokkaido (Japan) and found 11 samples with high activity. We then selected Elfvingia applanata and Fuscoporia obliqua for extraction and purification as these samples were able to suppress Aβ-induced neurocytotoxicity and were readily available in large quantities. We found that the ethyl acetate (EtOAc) extract of E. applanata has high Aβ aggregation inhibitory activity, so we performed silica gel column chromatography fractionation and found that fraction 5 (f5) of the EtOAc extract displayed the highest Aβ aggregation inhibitory activity among all mushroom samples. The half-maximal effective concentration (EC50) value was 2.30 µg/mL, higher than the EC50 of 10.7 µg/mL for rosmarinic acid, a well-known Aβ aggregation inhibitor. This inhibitory activity decreased with further purification, suggesting that some compounds act synergistically. The f5 fraction also inhibited the deposition of Aβ aggregates on the cell surface of human neuroblastoma SH-SY5Y cells. Our expectation is that f5, with additional tests, may eventually prove to be an inhibitor for the prevention of AD.
• Testing the Simplified Molecular Dynamics Approach to Improve the Reproduction of ECD Spectra and Monitor Aggregation
  Attila Mándi; Aliz Rimóczi; Andrea Vasas; Judit Hohmann; Mahadeva M. M. Swamy; Kenji Monde; Roland A. Barta; Máté Kicsák; István Komáromi; Krisztina Fehér; Tibor Kurtán
  International Journal of Molecular Sciences, 25, 12, 6453, 6453, MDPI AG, 12 Jun. 2024, [Peer-reviewed]
  Scientific journal, A simplified molecular-dynamics-based electronic circular dichroism (ECD) approach was tested on three condensed derivatives with limited conformational flexibility and an isochroman-2H-chromene hybrid, the ECD spectra of which could not be precisely reproduced by the conventional ECD calculation protocol. Application of explicit solvent molecules at the molecular mechanics (MD) level in the dynamics simulations and subsequent TDDFT-ECD calculation for the unoptimized MD structures was able to improve the agreements between experimental and computed spectra. Since enhancements were achieved even for molecules with limited conformational flexibility, deformations caused by the solvent molecules and multitudes of conformers produced with unoptimized geometries seem to be key factors for better agreement. The MD approach could confirm that aggregation of the phenanthrene natural product luzulin A had a significant contribution to a specific wavelength range of the experimental ECD. The MD approach has proved that dimer formation occurred in solution and this was responsible for the anomalous ECD spectrum. The scope and limitations of the method have also been discussed.
• Biocompatible and Water-Soluble Shortwave-Infrared (SWIR)-Emitting Cyanine-Based Fluorescent Probes for In Vivo Multiplexed Molecular Imaging
  Mahadeva M. M. Swamy; Yuta Murai; Kenji Monde; Setsuko Tsuboi; Aravind K. Swamy; Takashi Jin
  ACS Applied Materials & Interfaces, 16, 17253, 17266, American Chemical Society (ACS), 01 Apr. 2024, [Peer-reviewed]
  English, Scientific journal
• Stereochemistry‐activity relationship of ceramide‐induced exosome production to clear amyloid‐β in Alzheimer's disease
  Mariam Abdelrasoul; Kohei Yuyama; Mahadeva M. M. Swamy; Yuta Murai; Kenji Monde
  Chirality, 35, 9, 577, 585, Apr. 2023, [Peer-reviewed]
  Scientific journal
• Stereochemistry of Sphingolipids in Ganglioside GM3 Enhances Recovery of Nervous Functionality
  Sajeer Koolath; Yuta Murai; Tomoya Suzuki; Mahadeva M; M. Swamy; Seigo Usuki; Kenji Monde
  ACS Med. Chem. Lett., in press, 9, 1237, 1241, American Chemical Society (ACS), 2023, [Peer-reviewed]
  English, Scientific journal
• A near-infrared fluorescent long-chain fatty acid toward optical imaging of cardiac metabolism in living mice
  Mahadeva M. M. Swamy; Mohamad Zarif Mohd; Zubir; Mutmainah; Setsuko Tsuboi; Yuta Murai; Kenji Monde; Ken-ichi Hiranoc; Takashi Jin
  Analyst, 147, 19, 4206, 4212, Aug. 2022, [Peer-reviewed]
  Scientific journal
• Shortwave-infrared (SWIR) emitting annexin V for high-contrast fluorescence molecular imaging of tumor apoptosis in living mice
  Mahadeva M. M. Swamy; Setsuko Tsuboi; Yuta Murai; Kenji Monde; Takashi Jin
  RSC Advances, 12, 30, 19632, 19639, Jun. 2022, [Peer-reviewed]
  English, Scientific journal
• Shortwave-Infrared fluorescent molecular imaging probes based on π-conjugation extended indocyanine green
  Mahadeva M. M. Swamy, Yuta Murai, Kenji Monde, Setsuko Tsuboi, Takashi Jin
  Bioconjugate Chemistry, 32, 8, 1541, 1547, 18 Aug. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Recently, shortwave-infrared (SWIR) fluorescence imaging for the optical diagnostics of diseases has attracted much attention as a new noninvasive imaging modality. For this application, the development of SWIR molecular imaging probes with high biocompatibility is crucial. Although many types of biocompatible SWIR fluorescent probes based on organic dyes have been reported, there are no SWIR-emitting molecular imaging probes that can be used for the detection of specific biomolecules in vivo. To apply SWIR-emitting molecular imaging probes to biomedical fields, we developed a biocompatible SWIR fluorescent dye based on π-conjugation extended indocyanine green (ICG), where ICG is the only approved near-infrared dye by the US Food and Drug Administration (FDA) for use in the clinic. Using the π-conjugation extended ICG, we prepared SWIR molecular imaging probes that can be used for in vivo tumor imaging. Herein, we demonstrate noninvasive SWIR fluorescence imaging of human epidermal growth factor receptor 2 (HER2)-positive and epidermal growth factor receptor (EGFR)-positive breast tumors using π-conjugation extended ICG and monoclonal antibody conjugates. The presented π-conjugation extended ICG analog probes will be a breakthrough to apply SWIR fluorescence imaging in biomedical fields.
• Daurichromenic acid from the Chinese traditional medicinal plant Rhododendron dauricum inhibits sphingomyelin synthase and Aβ aggregation
  Hadya Virupaksha Deepak; Mahadeva M. M. Swamy; Yuta Murai; Yoshiko Suga; Masaki Anetai; Takuro Yo; Masahiro Kuragano; Koji Uwai; Kiyotaka Tokuraku; Kenji Monde
  Molecules, 25, 18, 4077, 4088, 07 Sep. 2020, [Peer-reviewed]
• Determination of the absolute configurations and sensory properties of the enantiomers of a homologous series (C6-C10) of 2-mercapto-4-alkanones
  Christiane Kiske; Anja Devenie Riegel; Ronja Hopf; Anna Kvind; Iulia Poplacean; Tohru Taniguchi; Mahadeva M. M. Swamy; Kenji Monde; Wolfgang Eisenreich; Karl-Heinz Engel
  J. Agric. Food Chem., 64, 4, 1187, 1196, 03 Jan. 2019, [Peer-reviewed]
  Scientific journal
• Structure-inspired design of a sphingolipid mimic sphingosine-1-phosphate receptor agonist from a naturally occurring sphingomyelin synthase inhibitor
  Mahadeva M. M. Swamy; Yuta Murai; Yusuke Ohno; Akio Kihara; Susumu Mitsutake; Yasuyuki Igarashi; Jian Yu; Min Yao; Yoshiko Suga; Masaki Anetai; Kenji Monde
  Chem. Commun., 54, 90, 12758, 12761, 08 Nov. 2018, [Peer-reviewed]
  Scientific journal
• Chemical and genetic rescue of an ep300 knockdown model for Rubinstein Taybi Syndrome in zebrafish
  Aswini Babu; Mageshi Kamaraj; Moumita Basu; Debanjan Mukherjee; Shruti Kapoor; Shashi Ranjan; Mahadeva M. M. Swamy; Stephanie Kaypee; Vinod Scaria; Tapas K. Kundu; Chetana Sachidanandan
  Biochim. Biophys. Acta, 1864, 4, 1203, 1215, Apr. 2018, [Peer-reviewed]
  Scientific journal
• Inhibition of histone/lysine acetyltransferase activity kills CoCl2-treated and hypoxia-exposed gastric cancer cells and reduces their invasiveness
  Suvasmita Rath; Lopamudra Das; Shrikant Babanrao Kokate; Nilabh Ghosh; Pragyesh Dixi; Niranjan Rou; Shivaram P. Singh; Subhasis Chattopadhyay; Hassan Ashktorab; Duane T. Smoo; Mahadeva M. M. Swamy; Tapas K. Kundu; Sheila E. Crowe; Asima Bhattacharyya
  Int. J. Biochem. Cell Biol., 82, 28, 40, Jan. 2017, [Peer-reviewed]
  Scientific journal
• Reducing molecular flexibility by cyclization for elucidation of absolute configuration by CD calculations: Daurichromenic acid
  Attila Mandi; Mahadeva M. M. Swamy; Tohru Taniguchi; Masaki Anetai; Kenji Monde
  Chirality, 28, 6, 453, 459, Jun. 2016, [Peer-reviewed]
  Scientific journal
• Stereochemistry of a rhododaurichromanic acid derivative
  Mahadeva M. M. Swamy; Attila Mándi; Masaki Anetai; Kenji Monde
  Nat. Prod. Commun., 11, 2, 193, 195, Feb. 2016, [Peer-reviewed]
  Scientific journal
• Hydrazinobenzoylcurcumin inhibits androgen receptor activity and growth of castration-resistant prostate cancer in mice
  Min Wu; Sahn-Ho Kim; Indrani Datta; Albert Levin; Gregory Dyson; Jing Li; Stephanie Kaypee; Mahadeva M. M. Swamy; Nilesh Gupta; Ho Jeong Kwon; Mani Menon; Tapas K. Kundu; G. Prem-Veer Reddy
  Oncotarget, 6, 8, 6136, 6150, Mar. 2015, [Peer-reviewed]
  Scientific journal
• Garcinol sensitizes human head and neck carcinoma to cisplatin in a xenograft mouse model despite downregulation of proliferative biomarkers
  Feng Li; Muthu K; Shanmugam; Kodappully Sivaraman Siveen; Fan Wang; Tina H. Ong; Ser Yue Loo; Mahadeva M. M. Swamy; Somnath Mandal; Alan Prem Kumar; Boon Cher Goh; Tapas Kundu; Kwang Seok Ahn; Ling Zhi Wang; Kam Man Hui; Gautam Sethi
  Oncotarget, 6, 7, 5147, 5163, Mar. 2015, [Peer-reviewed]
  Scientific journal
• Naphthoquinone-mediated inhibition of lysine acetyltransferase KAT3B/p300, basis for non-toxic inhibitor synthesis
  Mohankrishna Dalvoy Vasudevarao; Pushpak Mizar; Sujata Kumari; Somnath Mandal; Soumik Siddhanta; Mahadeva M. M. Swamy; Stephanie Kaypee; Ravindra C Kodihalli; Amrita Banerjee; Chandrabhas Naryana; Dipak Dasgupta; Tapas K. Kundu
  J. Biol. Chem., 289, 11, 7702, 7717, Mar. 2014, [Peer-reviewed]
  Scientific journal

• Garcinol and its role in chronic diseases
  Amit K. Behera, Mahadeva M. M. Swamy, Nagashayana, Tapas K. Kundu
  Adv. Exp. Med. Biol., Sep. 2016

• COMPOUND, PRODUCTION METHOD THEREFOR, COMPLEX, AND SHORT WAVELENGTH INFRARED FLUORESCENT AGENT
  Patent right, Takashi Jin; Mahadeva Swamy M. M.; Kenji Monde; Yuta Murai
  US20240174645A1, May 2024
• COMPOUND, PRODUCTION METHOD THEREFOR, COMPLEX, AND SHORT WAVELENGTH INFRARED FLUORESCENT AGENT
  Patent right, Takashi Jin; Mahadeva Swamy M. M.; Kenji Monde; Yuta Murai, RIKEN
  WO2022196694, Sep. 2022
• Complexes and shortwave-infrared fluorescent probes, manufacturing methods and thereof
  Patent right, Jin Takashi; M. M. Mahadeva Swamy; Monde Kenji; Murai Yuta, RIKEN; Hokkaido University
  特願2021-041717, 2021
• Daurichromenic acid, naturally occurring SMS inhibitor
  Patent right, Monde Kenji; Murai Yuta; M. M. Mahadeva Swamy, Hokkaido University
  特願2017-236847, 2017
• Ginkgolic acid, naturally occurring SMS inhibitor
  Patent right, Monde Kenji; Murai Yuta; M. M. Mahadeva Swamy, Hokkaido University
  特願2017-236846, 2017