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YOSHIMURA Yoshitaka

Faculty of Dental Medicine　Division of Dental Medicine　Department of Pathobiological ScienceAssociate Professor

Researcher basic information

■ Degree
  • 1995 Ph.D. Hokkaido University, Graduate School of Dentistry, Hokkaido University
■ URL
researchmap URLホームページURL■ Various IDs
Researcher number
  • 30230816
J-Global ID■ Research Keywords and Fields
Research Keyword
  • mechanical stress
  • osteonecrosis of the jaw
  • 免疫学
  • 機能系基礎歯科学
  • 歯根吸収
  • 破歯細胞
  • 乳歯
  • Dental Pharmacology
  • root resorption
  • odontoclast
  • deciduous teeth
Research Field
  • Life Science, Surgical dentistry
  • Life Science, Immunology
  • Life Science, Oral biological science
  • Life Science, Developmental dentistry
■ Educational Organization

Career

■ Career
Career
  • Jun. 2019 - Present
    Faculty of Dental Medicine and Graduate School of Dental Medicine, Hokkaido University, Department of Pharmacology,, Associate professor
  • Apr. 2017 - May 2019
    北海道大学大学院歯学研究院口腔病態学分野細胞分子薬理学教室, 准教授
  • 2014 - 2019
    Associate professor-Department of Oral Pathobiological Science, Graduate School of Dental Medicine, Hokkaido University
  • Nov. 2014 - Mar. 2017
    北海道大学大学院歯学研究科口腔病態学講座（細胞分子薬理学教室）, 准教授
  • Apr. 2007 - Oct. 2014
    北海道大学大学院歯学研究科口腔病態学講座（細胞分子薬理学教室）, 助教
  • 2007 - 2014
    Assistant professor-Department of Oral Pathobiological Science, Graduate School of Dental Medicine, Hokkaido University
  • Apr. 2000 - Mar. 2007
    北海道大学大学院歯学研究科（口腔病態学講座細胞分子薬理学分野）, 助手
  • 2000 - 2007
    Research Associate,Instructor-Department of Oral Pathobiological Science, Graduate School of Dental Medicine, Hokkaido University
  • Apr. 1990 - Mar. 2000
    北海道大学（歯学部歯科薬理学講座), 助手
  • 1990 - 2000
    Research Associate,Instructor-Department of Dental Pharmacology, School of Dentistry, Hokkaido University
Educational Background
  • Apr. 1984 - Mar. 1990, Hokkaido University, School of Dental Medicine, 歯学科
  • Apr. 1984 - Mar. 1990, Hokkaido University, Faculty of Dentistry, 1990 B.Sc., D.D.S. Hokkaido University, School of Dentistry
Committee Memberships
  • 2018 - Present
    歯科基礎医学会, 代議員, Society
  • May 2015 - Present
    北海道歯学会, 評議員, Society
  • 2003 - Present
    日本薬理学会, 評議員, Society

Research activity information

■ Papers
  • Hypoxia enhances osteoclastogenesis in periodontal ligament cells via expression of VEGF and RANKL
    Kenchi Hase; Misa Ishiyama; Shoko Ozawa; Yoshitaka Yoshimura; Takashi Kikuiri
    Pediatric Dental Journal, 35, 1, 100336, 100336, Elsevier BV, Apr. 2025, [Peer-reviewed]
    Scientific journal
  • Promoting Dentin Bridge Formation Through N-Acetyl-L-Cysteine Application in Rat Molar Pulpotomy: An Experimental Study
    Kota Takagi; Koichi Nakamura; Yoshitaka Yoshimura; Yasutaka Yawaka
    Journal of Functional Biomaterials, 16, 4, 117, 117, MDPI AG, 27 Mar. 2025, [Peer-reviewed]
    Scientific journal, Pulpotomy is performed when tooth decay reaches the dental pulp or when the crown is fractured due to trauma. Mineral trioxide aggregate (MTA) is commonly used in pulpotomy, but its prognosis can be variable. N-acetyl-L-cysteine (NAC), an antioxidant amino acid, has garnered attention due to its potential benefits. This study aimed to investigate the effects of MTA and NAC on pulpotomy outcomes. We used Sprague Dawley rat maxillary molars to perform pulpotomy and employed Superbond C&B, MTA, and MTA mixed with NAC (MTA–NAC) for pulp capping. We obtained tissue sections 3 and 7 days postpulpotomy, conducting histological analysis by examining the morphology of pulp tissue and assessing dentin sialophosphoprotein (DSPP) and osteopontin expression levels. At 3 days postpulpotomy, MTA and MTA–NAC reduced the inflammatory response. At 7 days postpulpotomy, dentin bridge formation was observed following MTA–NAC application, and although MTA resulted in DSPP- and osteopontin-positive areas, these areas were more extensive following MTA–NAC application. Given that adding NAC to MTA enhanced dentin bridge formation, MTA–NAC appears to be a superior option for pulp capping.
  • エストロゲンによる異なった受容体を介するマクロファージ様細胞のNa, K-ATPase活性促進作用
    平沢宏太; 出山義昭; 吉村善隆; 野谷健治; 鈴木邦明
    北海道歯学雑誌, 44, 16, 25, Sep. 2023, [Peer-reviewed]
    Scientific journal
  • 骨芽細胞の増殖，分化と石灰化におけるNa,K-ATPaseの役割
    山田淳一; 出山義昭; 吉村善隆; 鈴木邦明; 八若保孝
    北海道歯学雑誌, 44, 26, 36, Sep. 2023, [Peer-reviewed]
    Scientific journal
  • Na,K-ATPase活性のフッ素による阻害
    沖野雄一郎; 出山義昭; 吉村善隆; 鈴木 邦明; 本多, 丘人
    北海道歯学雑誌, 44, 37, 48, Sep. 2023, [Peer-reviewed]
    Scientific journal
  • RAW細胞の継代数が破骨細胞分化誘導系に与える影響
    栁澤瞳子; 吉村善隆; 早川貴子; 福島久夢; 飯村忠浩; 佐藤嘉晃
    北海道歯学雑誌, 43, 29, 35, Sep. 2022, [Peer-reviewed], [Corresponding author], [Domestic magazines]
    Japanese, Scientific journal
  • Lactoferrin directly suppresses osteoclastogenesis
    Hiromichi Kato; Yoshitaka Yoshimura; Hajime Minamikawa; Kuniaki Suzuki; Yutaka Yamazaki
    Hokkaido Journal of Dental Science, 43, 49, 56, Sep. 2022, [Peer-reviewed], [Corresponding author], [Domestic magazines]
    English, Scientific journal
  • 口腔がん細胞のシスプラチン耐性化に伴う白金系抗がん剤及び強心配糖体に対する感受性の変化
    義達理恵子; 鈴木邦明; 吉村善隆; 南川元; 鄭漢忠
    北海道歯学雑誌, 43, 29, 35, Sep. 2022, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal
  • Mg，Ca及びZnのヒトアルカリ性ホスファターゼ活性に対する作用
    半谷純一; 鈴木邦明; 吉村善隆; 南川元; 兼平孝; 本多丘人
    北海道歯学雑誌, 43, 9, 18, Sep. 2022, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal
  • A rare case of multiple brain abscesses caused by apical periodontitis of deciduous teeth in congenital heart disease: a case report.
    Shizuka Takahashi; Hisato Segoe; Takashi Kikuiri; Yuji Maruo; Tomonobu Sato; Yutaka Watanabe; Zhao Jimei; Yoshitaka Yoshimura; Misa Ishiyama; Atsuhito Takeda; Yasutaka Yawaka; Tetsuo Shirakawa
    BMC oral health, 22, 1, 261, 261, 28 Jun. 2022, [Peer-reviewed], [International Magazine]
    English, Scientific journal, BACKGROUND: A brain abscess is a focal infection in which abscesses form in the brain. A brain abscess is a rare but fatal disease when rupture occurs into the ventricles. We report a case of multiple brain abscesses caused by a hematogenous infection from the apical periodontitis of deciduous teeth. CASE PRESENTATION: The patient was a 7-years and 8-months-old male with congenital heart disease. The patient sought medical attention due to fever and headache, for which he was started on three antibiotics with a diagnosis of multiple brain abscesses. Given that apical periodontitis of deciduous teeth was strongly suspected as the source of the brain abscess, the deciduous teeth were extracted. Immediately after deciduous teeth extraction, the patient's headache and neurological symptoms disappeared. CONCLUSIONS: After teeth extraction, a clear shrinkage of the brain abscess was observed, and the patient was discharged from the hospital.
  • Osteoclast formation from mouse bone marrow cells on micro/nano-scale patterned surfaces
    Tsukasa Akasaka; Hiroshi Hayashi; Miho Tamai; Yoshitaka Yoshimura; Yoh-ichi Tagawa; Hirofumi Miyaji; Ko Nakanishi; Yasuhiro Yoshida
    Journal of Oral Biosciences, 64, 2, 237, 244, Elsevier BV, Jun. 2022, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • Different micro/nano-scale patterns of surface materials influence osteoclastogenesis and actin structure
    Tsukasa Akasaka; Miho Tamai; Yoshitaka Yoshimura; Natsumi Ushijima; Shinichiro Numamoto; Atsuro Yokoyama; Hirofumi Miyaji; Ryo Takata; Shuichi Yamagata; Yoshiaki Sato; Ko Nakanishi; Yasuhiro Yoshida
    Nano Research, 15, 5, 4201, 4211, Springer Science and Business Media LLC, May 2022, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Abstract

    The surface topography of a material can influence osteoclast activity. However, the surface structural factors that promote osteoclast activity have not yet been investigated in detail. Therefore, we investigated osteoclastogenesis by testing various defined patterns with different dimensions and shapes. The systematic patterns, made of a cyclo-olefin polymer, were prepared at a micron-, submicron-, and nano-scale with a groove, hole, or pillar shape with a 1:1 pitch ratio. RAW264.7 cells were cultured on these patterns in the presence of the receptor activator of NF-κB ligand (RANKL). Osteoclast formation was induced in the order: pillar > groove ≥ hole. The two-dimensional factors also indicated that submicron-sized patterns strongly induced osteoclast formation. The optimal pillar dimension for osteoclast formation was 500 nm in diameter and 2 µm in height. Furthermore, we observed two types of characteristic actin structure, i.e., belt-like structures with small hollow circles and isolated ring-like structures, which formed on or around the pillars depending on size and height. Furthermore, resorption pits were observed mainly on the top of calcium phosphate-coated pillars. Thus, osteoclasts prefer convex shapes, such as pillars for differentiation and resorption. Our results indicate that osteoclastogenesis can be controlled by designing surfaces with specific morphologies.
  • Anti-RANKL Inhibits Thymic Function and Causes DRONJ in Mice
    Yusuke Nakamura; Takashi Kikuiri; Takahiro Sugiyama; Ayako Maeda; Daisuke Izumiyama; Daigo Yahata; Yoshitaka Yoshimura; Tetsuo Shirakawa; Yoshimasa Kitagawa
    International Journal of Dentistry, 2022, 1, 9, Hindawi Limited, 15 Apr. 2022, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Background. Denosumab, a human monoclonal antibody against receptor activator of nuclear factor-kappa B ligand (RANKL), is a novel bone antiresorptive agent used in patients with osteoporosis or metastatic bone cancer. Denosumab-related osteonecrosis of the jaw (DRONJ) has been recently reported in patients using denosumab. However, the mechanisms of DRONJ are not fully understood. Appropriate pathogenic mechanisms of DRONJ have yet to be established. Therefore, we investigated the pathogenesis of DRONJ in mice. Methods. Anti-mouse RANKL monoclonal antibody and melphalan were performed to create a mouse model of DRONJ-like lesions in female C57BL/6J mice. We examined the development of DRONJ-like lesions and immune function. Results. We showed that administration of anti-mouse RANKL monoclonal antibody and melphalan caused DRONJ-like lesions that recapitulated major clinical manifestations of the human disease, including the characteristic features of an open alveolar socket and exposed necrotic bone. In the analysis using a mouse model of DRONJ-like lesion, it was revealed that anti-mouse RANKL monoclonal antibody and melphalan suppress autoimmune regulator (AIRE) expression in the thymus and imbalanced T cell populations. Conclusion. This study suggests evidence of an immunity-based mechanism of DRONJ-like disease. This work may contribute to a better understanding of the pathogenesis of human DRONJ.
  • Bone resorption improvement by conditioned medium of stem cells from human exfoliated deciduous teeth in ovariectomized mice
    Ayako Maeda; Takashi Kikuiri; Yoshitaka Yoshimura; Yasutaka Yawaka; Tetsuo Shirakawa
    Experimental and Therapeutic Medicine, 23, 4, Spandidos Publications, 21 Feb. 2022, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • Occurrence of subcutaneous emphysema during a caries filling procedure: A case report
    Takashi Kikuiri; Kureha Saito; Airi Iida; Yoshitaka Yoshimura; Yasutaka Yawaka; Tetsuo Shirakawa
    Pediatric Dental Journal, 2022, [Peer-reviewed]
    English, Scientific journal
  • エチドロネートによるヒト骨型アルカリ性ホスファターゼ活性阻害に対する２価金属と基質の影響
    島田英知; 鈴木邦明; 吉村善隆; 南川元; 山崎裕
    北海道歯学雑誌, 42, 36, 43, Sep. 2021, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal
  • 骨芽細胞様細胞（MC3T3-E1）のthapsigargin感受性Ca依存ATPaseの分離
    工藤智也; 出山義昭; 吉村善隆; 鈴木邦明; 山崎裕
    北海道歯学雑誌, 42, 28, 35, Sep. 2021, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal
  • 腫瘍及び非腫瘍細胞由来アルカリ性ホスファターゼの阻害剤に対する反応性の相違
    川村良; 出山義昭; 吉村善隆; 鈴木邦明; 山崎裕
    北海道歯学雑誌, 42, 19, 27, Sep. 2021, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal
  • N-acetylcysteine attenuates PGE2 and ROS production stimulated by 4-META/MMA-based resin in murine osteoblastic cells.
    Koichi Nakamura; Hajime Minamikawa; Shizuka Takahashi; Yoshitaka Yoshimura; Yasutaka Yawaka
    Dental materials journal, 40, 3, 808, 812, 29 May 2021, [Peer-reviewed], [Domestic magazines]
    English, Scientific journal, This study examined the effects of N-acetylcysteine (NAC) on the inflammatory reactions of murine osteoblastic cells cultured on the 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate (4-META/MMA)-based resin. Superbond C&B (SB) was used as the 4-META/MMA-based resin and placed in a 48-well cell culture plate. The cells were cultured in αMEM (control) as well as on SB and SB in αMEM with NAC (SB+NAC). They were examined using the WST-1 proliferation assay, real-time PCR, enzyme-linked immunosorbent assay (ELISA), intracellular reactive oxygen species (ROS) measurements, and cellular glutathione (GSH) detection. COX-2 and IL-6 gene expressions were upregulated in SB; however, they were suppressed by NAC. Furthermore, PGE2 production in the culture medium was increased in SB, whereas NAC decreased the PGE2 production. NAC lowered the ROS level in the culture medium and significantly increased the intracellular GSH level. The present in vitro study demonstrated that NAC might be effective for dental material detoxification.
  • Effects of N-acetylcysteine on root resorption after tooth replantation
    Mitsuhiko Nishimi; Koichi Nakamura; Akina Hisada; Kazuki Endo; Shuya Ushimura; Yoshitaka Yoshimura; Yasutaka Yawaka
    Pediatric Dental Journal, 30, 2, 72, 79, Elsevier BV, Aug. 2020, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • スダチチンは破骨細胞の分化誘導を抑制する
    萇崎誠治; 吉村善隆; 飯村忠浩; 山崎裕
    北海道歯学雑誌, 40, 2, 95, 104, Apr. 2020, [Peer-reviewed], [Corresponding author], [Domestic magazines]
    Japanese, Scientific journal
  • Edaravone suppresses osteoclastogenesis
    大西香織; 吉村善隆; 飯村忠浩; 山崎裕
    北海道歯学雑誌, 40, 2, 105, 113, Apr. 2020, [Peer-reviewed], [Corresponding author], [Domestic magazines]
    Japanese, Scientific journal
  • 間歇的な機械的刺激はRAW264.7細胞における破骨細胞分化を抑制する.
    加藤結香; 吉村善隆; 上村光太郎; 南川 元; 鈴木邦明; 飯田順一郎
    北海道歯学雑誌, 40, 1, 5, 12, Sep. 2019, [Peer-reviewed], [Corresponding author], [Domestic magazines]
    Japanese, Scientific journal
  • Patients with SATB2-associated syndrome exhibiting multiple odontomas
    Takashi Kikuiri; Hiroyuki Mishima; Hideto Imura; Satoshi Suzuki; Yusuke Matsuzawa; Takashi Nakamura; Satoshi Fukumoto; Yoshitaka Yoshimura; Satoshi Watanabe; Akira Kinoshita; Takahiro Yamada; Masanobu Shindoh; Yoshihiko Sugita; Hatsuhiko Maeda; Yasutaka Yawaka; Tadashi Mikoya; Nagato Natsume; Koh ichiro Yoshiura
    American Journal of Medical Genetics, Part A, 176, 12, 2614, 2622, Dec. 2018, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • 口腔がん細胞(KB)およびそのシスプラチン耐性株(KB-R)に対するネダプラチンおよびカルボプラチンの作
    義達理恵子; 鈴木邦明; 吉村善隆; 鄭 漢忠; 平野 正康
    北海道歯学雑誌, 39, 1, 11, 16, 北海道歯学会, Sep. 2018, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal
  • ラット及びウサギ脳Na, K-ATPase活性に対するbufadienolidesの作用
    李 加; 鈴木邦明; 蘇 韶懿; 南川 元; 吉村善隆
    北海道歯学雑誌, 38, 2, 227, 233, Mar. 2018, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal
  • 骨芽細胞様細胞（MC3T3-E1）のカルモジュリン依存性Ca-ATPase活性の性質害
    井坂一真; 鈴木邦明; 南川 元; 吉村善隆
    北海道歯学雑誌, 38, 2, 219, 226, Mar. 2018, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal
  • ビスホスホネートと２価金属イオンとの拮抗によるアルカリ性ホスファターゼ活性の阻害
    三上 翔; 鈴木邦明; 島田英知; 吉村善隆; 南川 元; 山崎 裕
    北海道歯学雑誌, 38, 2, 211, 218, Mar. 2018, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal
  • 持続的な機械的刺激はRAW264.7細胞における破骨細胞分化を抑制する
    上村光太郎; 吉村善隆; 南川 元; 鈴木 邦明; 飯田順一郎
    北海道歯学雑誌, 38, 2, 169, 176, Mar. 2018, [Peer-reviewed], [Corresponding author], [Domestic magazines]
    Japanese, Scientific journal
  • 圧縮力は培養後期における破骨細胞の分化・融合を抑制する
    宮上雄希; 吉村善隆; 南川 元; 鈴木邦明; 飯田順一郎
    北海道歯学雑誌, 38, 2, 131, 139, Mar. 2018, [Peer-reviewed], [Corresponding author], [Domestic magazines]
    Japanese, Scientific journal
  • Fusion of RAW 264.7 macrophage cells on micro-scale fine pillar patterns.
    Honma J; Akasaka T; Tamai M; Yoshimura Y; Taira T; Miyaji H; Yamagata S; Sato Y; Yoshida Y
    Digest Journal of Nanomaterials and Biostructures, 13, 4, 1123, 1131, 2018, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • Effect of a nano-scale fine hole pattern on the differentiation of RAW264.7 cells into osteoclasts.
    Takata R; Akasaka T; Tamai M; Yoshimura Y; Taira T; Miyaji H; Tagawa Y; Yamagata S; Iida J; Yoshida Y
    Digest Journal of Nanomaterials and Biostructures, 13, 2, 451, 458, 2018, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • Expression and dynamics of podoplanin in cultured osteoblasts with mechanostress and mineralization stimulus
    Tomohiro Takenawa; Takenori Kanai; Tetsuya Kitamura; Yoshitaka Yoshimura; Yoshihiko Sawa; Junichiro Iida
    Acta Histochemica et Cytochemica, 51, 1, 41, 52, Japan Society of Histochemistry and Cytochemistry, 2018, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • The difference in substrate selectivity and inhibitor sensitivity among human alkaline phosphatases
    更田恵理子; 出山義昭; 吉村善隆; 鈴木邦明; 山崎 裕
    北海道歯学雑誌, 38, 1, 48, 55, 北海道歯学会, Sep. 2017, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal, 各臓器由来のアルカリ性ホスファターゼ（ALP）の生体における真の基質と機能に関して未だに不明な点が多い．そこで，ヒトの骨型，小腸型，胎盤型及び肝臓由来のALPを購入して使用し，それらの基質選択性及び阻害剤に対する感受性を検索した．基質として，生体内には存在しないがALP活性の測定に汎用されるパラニトロフェニルリン酸（p -NPP）と，生体内に存在するナトリウムピロリン酸（Na-PPi），アデノシン三リン酸（ATP）及びピリドキサルリン酸（PLP）を使用して，各ALP活性のpH依存性と基質濃度依存性、及び阻害剤であるlevamisole及びvanadateによる阻害を検討した．活性は各基質が加水分解されて生ずる無機リンをChifflet法によって定量して測定した．骨型ALPの生体内基質とされているピロリン酸分解の至適pHはどのALPも8.8から9.1であり，また50%活性化濃度（K0.5）もALPの種類によらず3から5 mMであった．またピロリン酸を基質とした際の阻害剤の50%活性阻害濃度（Ki0.5）も類似しており，ALPの種類による違いは見いだせなかった．小腸型ALPの基質とされるPLP分解の至適pHはどのALPも9.9から10.4であり，またPLP分解のK0.5もALPの種類によらず1.1から1.6 mMであった． PLPを基質とした際の阻害剤のKi0.5は，vanadateではALPの種類によらず類似していたが，levamisoleでは異なっていた．ATPあるいはp -NPPを基質として同様の実験を行った結果，基質に対する至適pHと基質に対するK0.5はALPの由来には関係なく基質によって類似していた．また，levamisoleなどの阻害剤に対するKi0.5は基質の種類には関係なく，ALPの種類によってほぼ一定であった．ヒトに存在する各臓器由来のALPはその機能と生体内基質に対応して変化してきた可能性があると考えて本研究を行ったが，少なくとも今回検討した基質に関してはALPの種類による相違は見いだせず，むしろ阻害剤に対する感受性にALPの種類による相違が見られた．The human alkaline phosphatase (ALP), is homologous to not only other mammal ALP but also bacterial ALP. However, the substrate and function of ALP in vivo have not been cleared. Recently, we obtained human ALP as a chemical agent. In this study, we examined the differences in substrate selectivity and inhibitor sensitivity among several types of human ALP. We used 4 types of human ALP from bone, intestine, placenta, and liver. Also used were p -nitrophenyl phosphate (p -NPP), sodium pyrophosphate (Na-PPi), adenosine triphosphate (ATP) or pyridoxal phosphate (PLP) for substrate. The amount of inorganic phosphate as a result of hydrolysis by ALP was measured by Chifflet methods under different pH or substrate concentration. To examine the inhibitory effect on activity, levamisole or vanadate were used. The substrate concentration exhibiting 50% activity and the concentration exhibiting 50% inhibition were proposed as K0.5 and Ki0.5 respectively. When Na-PPi, the substrate of bone ALP, was used, the optical pH and K0.5 did not show differences among each of the ALP types (optical pH : 8.8 ～ 9.1, K0.5 : 3 ～ 5 mM). In the case of PLP, the substrate of intestine ALP, the optical pH and K0.5 did not show differences among each of the ALP types (optical pH : 9.9 ～ 10.4, K0.5 : 1.1 ～ 1.6 mM), although the optical pH shifted to the alkaline side. Additionally, the optical pH and K0.5 on p -NPP, which is not in the human body, but many researchers use it, did not show differences among any of the ALP types. However, in the case of PLP, Ki0.5 of levamisole was different among each of the ALP types, although Ki0.5 of levamisole or vanadate did not show any differences among the ALP types. We found that human ALP showed different inhibitor sensitivity among several ALP types, although the substrate selectivity was not determined.
  • Change of Na, K-ATPase and ouabain sensitivity in oral cancer cells accompanied by acquisition of cisplatin resistance
    平川 直; 出山義昭; 吉村善隆; 鈴木邦明; 鄭 漢忠
    北海道歯学雑誌, 38, 1, 40, 47, 北海道歯学会, Sep. 2017, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal, cis-diamminedichloro-platinum (II) （シスプラチン，CDDP）による癌の化学療法においては癌細胞の耐性化が障害となるが，その機序には不明な点も多い。そこで，口腔癌細胞株（Sa3，H1，KB）及びそれらのCDDP耐性株（Sa3R，H1R，KBR）と正常歯肉から採取した細胞を用いて，口腔癌細胞のCDDP耐性化に伴うNa,KATPaseの変化を中心として検討した． CDDPによる 50％細胞生存濃度（IC50）は，H1とH1Rではそれぞれ1.5×10-4及び7.5×10-4 M，Sa3とSa3Rでは4.5×10-3及び5.5×10-3 M，KBとKBRでは1.0×10-4及び7.0×10-4 Mであり，正常歯肉細胞は5.0×10-5 Mであった．癌細胞は正常歯肉細胞に比べてCDDP感受性が低く、耐性株はそれぞれの感受性株より低い感受性を示した．Na,K-ATPase活性は，Sa3よりSa3Rが高く，H1よりH1Rがわずかに高値を示したが，KBとKBRで差はなかった．Ouabain存在下での各細胞のIC50は、H1とH1Rでは5及び20 nM，Sa3とSa3Rでは80及び180 nM，KBとKBRでは40及び130 nMであり，正常歯肉細胞は5 nMであった．この結果から，CDDP耐性化はouabainに対する耐性化も伴うことが示唆された．各親株と耐性株間のNa,K-ATPase活性阻害に対するouabain濃度依存性は細胞によって異なり，ouabainに対する耐性化はNa, K-ATPaseの変化によるものではないと示唆された．各細胞のP-糖タンパク質とATP7Aの発現を親株と耐性株間で比較したところ，発現量は細胞によって異なった．以上の結果から，CDDP耐性化のメカニズムは細胞の種類によって異なることと，CDDP耐性化はouabain耐性化を伴うことが示唆された．Purpose : Resistance of cancer cells is an obstacle in chemotherapy for cancer by cisplatin (CDDP). But it is not well elucidated in the mechanism. We examined the change of Na, K-ATPase accompanied by the acquisition of the CDDP resistance of oral cancer cells.Methods : Oral cancer cell lines (Sa3, H1, KB) and CDDP-resistant lines of (Sa3R, H1R, KBR) provided from Chiba University, Faculty of Medicine and cells collected from normal gingiva, were used. Na, K-ATPase activity and cell viability under treatment ouabain and CDDP for each cell was measured. Assessment of Na, K-ATPase α subunits, ATP7A and P-glycoprotein (P-gp) was done by western blotting.Results : According to CDDP, 50% inhibitory concentration (IC50) was, 7.5 × 10-4 M and 1.5 × 10-4 M respectively in H1R and H1, 6.0×10-3 M and 4.5×10-3 M respectively in Sa3R and Sa3, 7.0×10-4 M and 1.0×10-4 M respectively in KBR and KB. CDDP sensitivity was lower than the normal gingival cells to cancer cells. Resistant strains showed a lower sensitivity than the more sensitive strains of each. Measurement of Na, K-ATPase activity, Sa3R is higher than Sa3, H1R was higher than H1 slightly, but there was no significant difference in the KBR and KB. Activity measurements were consistent in Na, K-ATPase and protein expression levels. IC50 was examined for each cell in the presence of ouabain and were 80 nM and 60 nM in the H1R and H1, 180 nM and 80 nM in the Sa3R and Sa3, 130 nM and 40 nM in the KBR and KB. The normal gingival cells was 5 nM. From the results of these examinations, it was suggested the cancer cells resistant to CDDP get resistant to ouabain at the same time. As for the ouabain concentration dependence of Na, K-ATPase inhibition of the cells, there is no significant difference in the 50% inhibitory concentration between resistant and parent lines. Therefore, change of Na,KATPase sensitivity to ouabain was not related to resistance of the cells against ouabain. It was suggested that P-gp and Na, K-ATPase, ATP7A is involved in CDDP resistance. The mechanism depends on the type of cells, although we found no features that are common in the between the parent and resistant lines for the expression of ATP7A and P-gp.
  • Effects of intravenous anesthetics on the activities of various ATPases in rat brain
    宮本健志; 出山義昭; 吉村善隆; 鈴木邦明; 藤澤俊明
    北海道歯学雑誌, 38, 1, 28, 39, 北海道歯学会, Sep. 2017, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal, 静脈麻酔薬のATPaseに対する作用の報告は多いが，作用機構の詳細については不明な点が多い．ATPaseにはF型ATPase（ミトコンドリアATP合成酵素），P型ATPase，V型ATPase等が存在し，活性発現にMg2+（Mg）を必要とするが，役割や性質の不明なMg-ATPaseも多数報告されている．本研究ではラット脳に存在するMg-ATPaseを分析するとともに，静脈麻酔薬の作用を検討した． ラット全脳から，Pottorfの方法に従って，形質膜（PII）とミクロソーム（PIII）分画を得た．Mg-ATPase活性の至適pHはPIIが約9.4，PIIIは約7.4であった．PIIのMg-ATPase活性はF型ATPaseの阻害剤であるNaN3によって約80%抑制され，PIIIのMg-ATPase活性はP型ATPase及びV型ATPaseの阻害剤であるNa3VO4及びbafilomycineA1によってそれぞれ約10%及び30%抑制された．ウエスタンブロッティングの結果からも，F型ATPaseはPIIに，V型ATPaseはPIIIに最も多く検出された．上記阻害剤で抑制されないMg-ATPase活性をBasal Mg-ATPaseとし，各阻害剤の存在下で各ATPaseを分別して静脈麻酔薬の影響を調べた．PropofolはV型ATPase活性，PII及びPIII Basal Mg-ATPase活性を濃度依存的に抑制したが，F型ATPase活性は80%程度活性化した．PentobarbitalはすべてのATPase活性を濃度依存的に抑制した．ThiopentalもすべてのATPase活性を抑制したが，その程度は各ATPaseによって異なった． 以上の結果から，静脈麻酔薬はラット脳Mg-ATPase活性を基本的に抑制するが，propofolはミトコンドリアに特異的な作用を及ぼす事が示唆された．This study examined whether the activity of magnesium-dependent ATPase (Mg2+-ATPase) in rat brains is a target for intravenous anesthetics. The effects of propofol, pentobarbital, and thiopental on magnesium-dependent ATPase in the plasma membrane fraction (PII) and microsomal fraction (PIII) isolated from rat brains homogenates by the method of Pottorf were examined. The optimal pH values for Mg2+-ATPase activities in PII and PIII were 9.4 and 7.4, respectively. The Mg2+-ATPase activity of PII was inhibited by about 80% by NaN3 (sodium azide), an inhibitor of F-type ATPase. The Mg2+-ATPase activity of PIII was inhibited by about 30% by the V-type ATPase inhibitor bafilomycin, and by about 10% by the P-type ATPase inhibitor vanadate. Western blotting analysis revealed that the largest amount of F-type ATPase was detected in PII, and the largest amount of V-type ATPase was detected in PIII. We defined all ATPase activity that was insensitive to the above inhibitors as basal Mg2+-ATPase activity. In the presence of these inhibitors, we examined the effects of intravenous anesthetics on the activities of various ATPases. Propofol inhibits V-type ATPase activity, PII basal Mg2+-ATPase activity, and PIII basal Mg2+-ATPase activity in a dose-dependent manner; however, F-type ATPase activity was uniquely stimulated about 80% by propofol at concentrations below 0.6 mM. Pentobarbital inhibited all types of ATPases in a dose-dependent manner. Thiopental inhibited all types of ATPases to varying degrees. These results suggest that intravenous anesthetics basically inhibit Mg2+-dependent ATPase activities in rat brains, but propofol shows specific effects on mitochondrial ATPase activity.
  • How much medium do you use for cell culture? Medium volume influences mineralization and osteoclastogenesis in vitro
    Yoshitaka Yoshimura; Takashi Kikuiri; Tomokazu Hasegawa; Mino Matsuno; Hajime Minamikawa; Yoshiaki Deyama; Kuniaki Suzuki
    MOLECULAR MEDICINE REPORTS, 16, 1, 429, 434, Jul. 2017, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
    English, Scientific journal
  • ハイドロキシアパタイトブロックを用いた圧縮力がMC3T3-E1細胞に与える影響について
    前田輪嘉幹; 飯田俊二; 吉村善隆; 横山敦郎
    北海道歯学雑誌, 37, 2, 133, 140, Mar. 2017, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal
  • Release from optimal compressive force suppresses osteoclast differentiation
    Masaaki Ikeda; Yoshitaka Yoshimura; Takashi Kikuiri; Mino Matsuno; Tomokazu Hasegawa; Kumu Fukushima; Takako Hayakawa; Hajime Minamikawa; Kuniaki Suzuki; Junichiro Iida
    MOLECULAR MEDICINE REPORTS, 14, 5, 4699, 4705, Nov. 2016, [Peer-reviewed], [Corresponding author], [International Magazine]
    English, Scientific journal
  • Optimal compressive force accelerates osteoclastogenesis in RAW264.7 cells
    Takako Hayakawa; Yoshitaka Yoshimura; Takashi Kikuiri; Mino Matsuno; Tomokazu Hasegawa; Kumu Fukushima; Kenjiro Shibata; Yoshiaki Deyama; Kuniaki Suzuki; Junichiro Iida
    MOLECULAR MEDICINE REPORTS, 12, 4, 5879, 5885, Oct. 2015, [Peer-reviewed], [Corresponding author], [International Magazine]
    English, Scientific journal
  • Recruitment of mesenchymal stem cells by stromal cell-derived factor 1 alpha in pulp cells from deciduous teeth
    Yuki Akazawa; Tomokazu Hasegawa; Yoshitaka Yoshimura; Naoyuki Chosa; Takeyoshi Asakawa; Kimiko Ueda; Asuna Sugimoto; Takamasa Kitamura; Hiroshi Nakagawa; Akira Ishisaki; Tsutomu Iwamoto
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 36, 2, 442, 448, Aug. 2015, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • Rosmarinic acid and arbutin suppress osteoclast differentiation by inhibiting superoxide and NFATc1 downregulation in RAW 264.7 cells.
    Akina Omori; Yoshitaka Yoshimura; Yoshiaki Deyama; Kuniaki Suzuki
    Biomedical Report, 3, 483, 490, 2015, [Peer-reviewed], [Corresponding author]
    English, Scientific journal
  • Forced mastication increases survival of adult neural stem cells in the hippocampal dentate gyrus
    Yuki Akazawa; Takamasa Kitamura; Yuri Fujihara; Yoshitaka Yoshimura; Masato Mitome; Tomokazu Hasegawa
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 31, 2, 307, 314, Feb. 2013, [Peer-reviewed]
    English, Scientific journal
  • Short-term mechanical stress inhibits osteoclastogenesis via suppression of DC-STAMP in RAW264.7 cells
    Sumika Kameyama; Yoshitaka Yoshimura; Takeshi Kameyama; Takashi Kikuiri; Mino Matsuno; Yoshiaki Deyama; Kuniaki Suzuki; Junichiro Iida
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 31, 2, 292, 298, Feb. 2013, [Peer-reviewed], [Corresponding author], [International Magazine]
    English, Scientific journal
  • 圧縮力が破骨細胞分化に与える影響
    福島久夢; 吉村善隆; 出山義昭; 鈴木邦明; 飯田順一郎
    北海道歯学雑誌, 33, 94, 102, 2013, [Peer-reviewed], [Corresponding author], [Domestic magazines]
    Japanese, Scientific journal
  • 骨芽細胞様MC3T3-E1細胞の各種ATPaseに対するエストロゲンの作用
    孔 令群; 出山義昭; 工藤智也; 吉村善隆; 鈴木邦明
    北海道歯学雑誌, 33, 175, 184, 2013, [Peer-reviewed], [Domestic magazines]
    Japanese, Scientific journal
  • Differential effects of TGF-beta 1 and FGF-2 on SDF-1 alpha expression in human periodontal ligament cells derived from deciduous teeth in vitro
    Tomokazu Hasegawa; Naoyuki Chosa; Takeyoshi Asakawa; Yoshitaka Yoshimura; Yuri Fujihara; Takamasa Kitamura; Mitsuro Tanaka; Akira Ishisaki; Masato Mitome
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 30, 1, 35, 40, Jul. 2012, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • Influence of nanometer smoothness and fibronectin immobilization of titanium surface on MC3T3-E1 cell behavior
    Eiji Yoshida; Yoshitaka Yoshimura; Motohiro Uo; Masao Yoshinari; Tohru Hayakawa
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 100A, 6, 1556, 1564, Jun. 2012, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • Effects of bisphosphonates on osteoclastogenesis in RAW264.7 cells
    Keigo Abe; Yoshitaka Yoshimura; Yoshiaki Deyama; Takashi Kikuiri; Tomokazu Hasegawa; Kanchu Tei; Hisashi Shinoda; Kuniaki Suzuki; Yoshimasa Kitagawa
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 29, 6, 1007, 1015, Jun. 2012, [Peer-reviewed], [Corresponding author]
    English, Scientific journal
  • Fibroblast growth factor 2 inhibits the expression of stromal cell-derived factor 1 alpha in periodontal ligament cells derived from human permanent teeth in vitro
    Takeyoshi Asakawa; Naoyuki Chosa; Yoshitaka Yoshimura; Asami Asakawa; Mitsuro Tanaka; Akira Ishisaki; Masato Mitome; Tomokazu Hasegawa
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 29, 4, 569, 573, Apr. 2012, [Peer-reviewed]
    English, Scientific journal
  • Toll like receptor 5 ligand induces monocyte chemoattractant protein-1 in mouse osteoblastic cells
    Kimiya Nakamura; Yoshiaki Deyama; Yoshitaka Yoshimura; Kuniaki Suzuki; Manabu Morita
    BIOMEDICAL RESEARCH-TOKYO, 33, 1, 39, 44, Feb. 2012, [Peer-reviewed]
    English, Scientific journal
  • MC3T3-E1 cells on titanium surfaces with nanometer smoothness and fibronectin immobilization
    Tohru Hayakawa; Eiji Yoshida; Yoshitaka Yoshimura; Motohiro Uo; Masao Yoshinari
    International Journal of Biomaterials, 2012, 743465, 2012, [Peer-reviewed]
    English, Scientific journal
  • Mechanism for propofol inhibition of Na＋, K＋-ATPase activity in rat brain.
    Yuri Hase; Yoshiaki Deyama; Yoshitaka Yoshimura; Kuniaki Suzuki; Kazuaki Fukushima
    Hokkaido Journal of Dental Science, 32, 2, 147, 155, 2012, [Peer-reviewed]
    English, Scientific journal
  • ヒトアルカリ性ホスファターゼ・アイソザイムの阻害剤に対する感受性の相違
    飯岡拓馬; 出山義昭; 吉村善隆; 鈴木邦明
    北海道歯学雑誌, 32, 2, 202, 209, 2012, [Peer-reviewed]
    Japanese, Scientific journal
  • Inhibition of calcium-dependent ATPase activity in rat brain by intravenous anesthetics
    TANAKA Hiromitsu; DEYAMA Yoshiaki; YOSHIMURA Yoshitaka; SUZUKI Kuniaki; FUKUSHIMA Kazuaki
    北海道歯学雑誌, 32, 2, 222, 229, 北海道歯学会, 2012, [Peer-reviewed]
    Japanese, Scientific journal, 全身麻酔薬の作用を受ける機能性タンパク質としてGABA受容体，アセチルコリン受容体などの報告があるが，これらに対する作用のみで全身麻酔作用のすべてを説明することはできないと考えられている．本研究はラット脳に存在するカルシウム（Ca）で活性化されるATPaseが，静脈麻酔薬の直接の作用点となり得るかを検討することを目的に行なった．ラット脳のホモジェネートから調整した膜分画（PⅠ）及び，Pottorfの方法に従って調整した形質膜（PⅡ）及びミクロソーム（PⅢ）分画のCaで活性化されるATPaseに対するpropofol，pentobarbitalとthiopentalの作用を調べた．PⅠにはCaの濃度に依存して活性化されるATPaseが存在し１mM以上のCaでほぼ飽和した（Ca-ATPase活性）．PⅠのCa-ATPase活性はpropofol濃度に依存して抑制され50％阻害濃度（Ki0.5）は0.25 mMであった．Propofol存在下でCa-ATPase活性のCa濃度依存性を調べると，存在するpropofol濃度に依存して最大活性とCaに対するKmは低下した．PⅡ及びPⅢには，ウェスタンブロッティング及び酵素学的性質からCa,Mg-ATPaseであるPMCA（plasma membrane Ca-ATPase）及びSERCA（sarcoendoplasmic reticulum Ca-ATPase）の存在が明らかになった．PⅡ 及びPⅢ のCa,Mg-ATPase活性はいずれも類似した濃度依存性でpropofol，pentobarbitalとthiopentalによって阻害され，そのKi0.5はそれぞれ0.35，３及び1.5 mMであった．本研究で調べたCa-ATPase及びCa,Mg-ATPase活性のpropofol，pentobarbitalとthiopentalによる活性阻害濃度は，同じ生体膜に存在する酵素であるNa,K-ATPase活性の阻害濃度と類似していた．以上の結果から，ラット脳に存在するCaで活性化されるATPase活性の静脈麻酔薬による阻害は特異的なものではなく，麻酔薬の生体膜を構成する脂質とタンパク質にに対する作用の結果として，ATPaseタンパク質の機能が影響を受け抑制されたものと推測した．
  • Stage-dependent suppression of the formation of dentin-resorbing multinuclear cells with migration inhibitory factor in vitro
    Takashi Kikuiri; Yoshitaka Yoshimura; Futoshi Tabata; Tomokazu Hasegawa; Jun Nishihira; Tetsuo Shirakawa
    EXPERIMENTAL AND THERAPEUTIC MEDICINE, 3, 1, 37, 43, Jan. 2012, [Peer-reviewed], [Lead author, Corresponding author]
    English, Scientific journal
  • Platinum Nanoparticles Suppress Osteoclastogenesis Through Scavenging of Reactive Oxygen Species Produced in RAW264.7 Cells
    Mayumi Nomura; Yoshitaka Yoshimura; Takashi Kikuiri; Tomokazu Hasegawa; Yumi Taniguchi; Yoshiaki Deyama; Ken-ichi Koshiro; Hidehiko Sano; Kuniaki Suzuki; Nobuo Inoue
    JOURNAL OF PHARMACOLOGICAL SCIENCES, 117, 4, 243, 252, Dec. 2011, [Peer-reviewed], [Corresponding author]
    English, Scientific journal
  • Attachment Formation After Transplantation of Teeth Cultured With Enamel Matrix Derivative in Dogs
    Akira Saito; Emiko Saito; Yoshitaka Yoshimura; Dairo Takahashi; Ryohei Handa; Yoshiyuki Honma; Noboru Ohata
    JOURNAL OF PERIODONTOLOGY, 82, 10, 1462, 1468, Oct. 2011, [Peer-reviewed]
    English, Scientific journal
  • Effect of the release from mechanical stress on osteoclastogenesis in RAW264.7 cells
    Kenjiro Shibata; Yoshitaka Yoshimura; Takashi Kikuiri; Tomokazu Hasegawa; Yumi Taniguchi; Yoshiaki Deyama; Kuniaki Suzuki; Junichiro Iida
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 28, 1, 73, 79, Jul. 2011, [Peer-reviewed], [Corresponding author]
    English, Scientific journal
  • Tannin-fluoride preparation attenuates prostaglandin E-2 production by dental pulp cells
    Koichi Nakamura; Yoshiaki Deyama; Yoshitaka Yoshimura; Masanor Hashimoto; Masayuki Kaga; Kuniaki Suzuki; Yasutaka Yawaka
    MOLECULAR MEDICINE REPORTS, 4, 4, 641, 644, Jul. 2011, [Peer-reviewed]
    English, Scientific journal
  • Establishment of immortalized human periodontal ligament cells derived from deciduous teeth
    Tomokazu Hasegawa; Naoyuki Chosa; Takeyoshi Asakawa; Yoshitaka Yoshimura; Akira Ishisaki; Mitsuro Tanaka
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 26, 5, 701, 705, Nov. 2010, [Peer-reviewed]
    English, Scientific journal
  • Effect of fibroblast growth factor-2 on dental pulp cells derived from human deciduous teeth in vitro
    Tomokazu Hasegawa; Naoyuki Chosa; Takeyoshi Asakawa; Yoshitaka Yoshimura; Asami Asakawa; Akira Ishisaki; Mitsuro Tanaka
    EXPERIMENTAL AND THERAPEUTIC MEDICINE, 1, 3, 477, 480, May 2010, [Peer-reviewed]
    English, Scientific journal
  • Effect of fibroblast growth factor-2 on periodontal ligament cells derived from human deciduous teeth in vitro
    Tomokazu Hasegawa; Naoyuki Chosa; Takeyoshi Asakawa; Yoshitaka Yoshimura; Asami Asakawa; Akira Ishisaki; Mitsuro Tanaka
    EXPERIMENTAL AND THERAPEUTIC MEDICINE, 1, 2, 337, 341, Mar. 2010, [Peer-reviewed]
    English, Scientific journal
  • Regulation of Human and Pig Renal Na+,K+-ATPase Activity by Tyrosine Phosphorylation of Their alpha(1)-Subunits
    Waleed El-Beialy; Nadia Galal; Yoshiaki Deyama; Yoshitaka Yoshimura; Kuniaki Suzuki; Kanchu Tei; Yasunori Totsuka
    JOURNAL OF MEMBRANE BIOLOGY, 233, 1-3, 119, 126, Feb. 2010, [Peer-reviewed]
    English, Scientific journal
  • Neurons Preferentially Respond to Self-MHC Class I Allele Products Regardless of Peptide Presented
    Nathalie Escande-Beillard; Lorraine Washburn; Dan Zekzer; Zhongqi-Phyllis Wu; Shoshy Eitan; Sonia Ivkovic; Yuxin Lu; Hoa Dang; Blake Middleton; Tina V. Bilousova; Yoshitaka Yoshimura; Christopher J. Evans; Sebastian Joyce; Jide Tian; Daniel L. Kaufman
    JOURNAL OF IMMUNOLOGY, 184, 2, 816, 823, Jan. 2010, [Peer-reviewed]
    English, Scientific journal
  • Effects of Estrogen on PMCA 2 and 4 in Human Fibroblast-like Synovial Cells and Mouse Macrophage-like Cells
    Waleed El-Beialy; Nadia Galal; Yoshiaki Deyama; Yoshitaka Yoshimura; Kuniaki Suzuki; Kanchu Tei; Yasunori Totsuka
    ENDOCRINE JOURNAL, 57, 1, 93, 97, Jan. 2010, [Peer-reviewed]
    English, Scientific journal
  • 家兎下顎骨骨空洞の治癒過程におけるPLGA・コラーゲン・ハイブリッドメッシュの有用性の検討
    野呂洋輔; 大廣洋一; 鄭 漢忠; 吉村善隆; 出山義昭; 飯塚 正; 鈴木邦明; 戸塚靖則
    北海道歯学雑誌, 31, 62, 69, 2010, [Peer-reviewed]
    Japanese, Scientific journal
  • フッ素によるアルミニウムに依存したNa,K-ATPase活性の抑制
    石川一郎; 出山義昭; 吉村善隆; 鈴木邦明
    北海道歯学雑誌, 31, 44, 51, 2010, [Peer-reviewed]
    Japanese, Scientific journal
  • Up-regulation of the G3PDH 'housekeeping' gene by estrogen
    Nadia Galal; Waleed El-Beialy; Yoshiaki Deyama; Yoshitaka Yoshimura; Kanchu Tei; Kuniaki Suzuki; Yasunori Totsuka
    MOLECULAR MEDICINE REPORTS, 3, 1, 111, 113, Jan. 2010, [Peer-reviewed]
    English, Scientific journal
  • Effect of Mineral Trioxide Aggregate on Rat Clonal Dental Pulp Cells: Expression of Cyclooxygenase-2 mRNA and Inflammation-related Protein via Nuclear Factor Kappa B Signaling System
    Hajime Minamikawa; Yoshiaki Deyama; Kouichi Nakamura; Yoshitaka Yoshimura; Masayuki Kaga; Kuniaki Suzuki; Yasutaka Yawaka
    JOURNAL OF ENDODONTICS, 35, 6, 843, 846, Jun. 2009, [Peer-reviewed]
    English, Scientific journal
  • Cisplatin sensitivity of oral squamous carcinoma cells is regulated by Na+,K+-ATPase activity rather than copper-transporting P-type ATPases, ATP7A and ATP7B
    Zunaid Ahmed; Yoshiaki Deyama; Yoshitaka Yoshimura; Kuniaki Suzuki
    CANCER CHEMOTHERAPY AND PHARMACOLOGY, 63, 4, 643, 650, Mar. 2009, [Peer-reviewed]
    English, Scientific journal
  • Mechanical stress up-regulates RANKL expression via the VEGF autocrine pathway in osteoblastic MC3T3-E1 cells
    Takeshi Nakai; Yoshitaka Yoshimura; Yoshiaki Deyama; Kuniaki Suzuki; Junichiro Iida
    MOLECULAR MEDICINE REPORTS, 2, 2, 229, 234, Mar. 2009, [Peer-reviewed], [Corresponding author]
    English, Scientific journal
  • 骨芽細胞様細胞のMg2+依存性ATPase活性の性質.
    齋藤豪紀; 出山義昭; 吉村善隆; 戸塚靖則; 鈴木邦明
    北海道歯学雑誌, 30, 61, 68, 2009, [Peer-reviewed]
    Japanese, Scientific journal
  • Synthetic double-stranded RNA induces retinoic acid-inducible gene-I in mouse osteoblastic cells
    Kimiya Nakamura; Yoshiaki Deyama; Yoshitaka Yoshimura; Kuniaki Suzuki; Manabu Morita
    MOLECULAR MEDICINE REPORTS, 1, 6, 833, 836, Nov. 2008, [Peer-reviewed]
    English, Scientific journal
  • Cellular expression of Bcl-2 and Bax in atrophic submandibular glands of rats
    Shigeru Takahashi; Yoshitaka Yoshimura; Tsuneyuki Yamamoto; Minoru Wakita
    INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, 89, 5, 303, 308, Oct. 2008, [Peer-reviewed]
    English, Scientific journal
  • Oral squamous cell carcinomas stimulate osteoclast differentiation
    Yoshiaki Deyama; Kanchu Tei; Yoshitaka Yoshimura; Yuri Izumiyama; Sadaaki Takeyama; Mitsutoki Hatta; Yasunori Totsuka; Kuniaki Suzuki
    ONCOLOGY REPORTS, 20, 3, 663, 668, Sep. 2008, [Peer-reviewed]
    English, Scientific journal
  • Effect of estrogen on bone resorption and inflammation in the temporomandibular joint cellular elements
    Nadia Galal; Waleed El Beialy; Yoshiaki Deyama; Yoshitaka Yoshimura; Tetsuya Yoshikawa; Kuniaki Suzuki; Yasunori Totsuka
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 21, 6, 785, 790, Jun. 2008, [Peer-reviewed]
    English, Scientific journal
  • Mechanical stress directly suppresses osteoclast differentiation in RAW264.7 cells
    Nobumitsu Suzuki; Yoshitaka Yoshimura; Yoshiaki Deyama; Kuniaki Suzuki; Yoshimasa Kitagawa
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 21, 3, 291, 296, Mar. 2008, [Peer-reviewed], [Corresponding author]
    English, Scientific journal
  • 胚性腫細胞株P19EC細胞の静脈麻酔薬による細胞死に関する研究
    今渡隆成; 出山義昭; 吉村善隆; 鈴木邦明; 福島和昭
    北海道歯学雑誌, 29, 61, 68, 2008, [Peer-reviewed]
    English, Scientific journal
  • 骨芽細胞様細胞のカルシウム依存ATPase活性に対するマンガン及び銅イオンの影響
    小林正明; 出山義昭; 鄭 漢忠; 吉村善隆; 戸塚靖則; 鈴木邦明
    北海道歯学雑誌, 29, 69, 77, 2008, [Peer-reviewed]
    English, Scientific journal
  • Cisplatin inhibits Na+, K+-ATPase activity depending on its concentration, preincubation time and temperature.
    Zunaid Ahmed; Yoshiaki Deyama; Yoshitaka Yoshimura; Kuniaki Suzuki
    Hokkaido Journal of Dental Science, 29, 1, 78, 86, 2008, [Peer-reviewed]
    English, Scientific journal
  • Novel effect of estrogen on RANK and c-fms expression in RAW 264.7 cells
    Nadia Galal; Waleed R. El-Beialy; Yoshiaki Deyama; Yoshitaka Yoshimura; Kuniaki Suzuki; Yasunori Totsuka
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 20, 1, 97, 101, Jul. 2007, [Peer-reviewed]
    English, Scientific journal
  • Toll-like receptor 3 ligand-induced antiviral response in mouse osteoblastic cells
    Kimiya Nakamura; Yoshiaki Deyama; Yoshitaka Yoshimura; Kuniaki Suzuki; Manabu Morita
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 19, 5, 771, 775, May 2007, [Peer-reviewed]
    English, Scientific journal
  • Participation of the Fas and Fas ligand systems in apoptosis during atrophy of the rat submandibular glands
    Shigeru Takahashi; Glenda C. Gobe; Yoshitaka Yoshimura; Takao Kohgo; Tsuneyuki Yamamoto; Minoru Wakita
    INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, 88, 1, 9, 17, Feb. 2007, [Peer-reviewed]
    English, Scientific journal
  • Continuous assay of protein tyrosine phosphatases based on fluorescence resonance energy transfer
    M. Nishikata; Y. Yoshimura; Y. Deyama; K. Suzuki
    BIOCHIMIE, 88, 7, 879, 886, Jul. 2006, [Peer-reviewed]
    English, Scientific journal
  • Molecular characterization of the zinc ringer transcription factor, Osterix
    M Hatta; Y Yoshimura; Y Deyama; A Fukamizu; K Suzuki
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 17, 3, 425, 430, Mar. 2006, [Peer-reviewed]
    English, Scientific journal
  • Partial purification of a calcium dependent ATPase of a clonal osteoblastic cell (MC3T3-E1)
    OBATA Makoto; DEYAMA Yoshiaki; YOSHIMURA Yoshitaka; FUKUSHIMA Kazuaki; SUZUKI Kuniaki
    北海道歯学雑誌, 27, 2, 190, 199, 2006, [Peer-reviewed]
    Japanese, Scientific journal
  • ヒト歯肉由来線維芽細胞におよぼす高濃度ニフェジピンの影響
    加藤晶久; 吉村善隆; 小田島朝臣; 川浪雅光
    北海道歯学雑誌, 27, 201, 210, 2006, [Peer-reviewed]
    English, Scientific journal
  • RANKLによるRAW264.7細胞の破骨細胞分化に伴うアポトーシスの誘導
    水野貴行; 吉村善隆; 出山義昭; 八田光世; 鈴木邦明; 北川義政
    北海道歯学雑誌, 26, 2, 210, 217, 北海道歯学会, 2005, [Peer-reviewed], [Corresponding author]
    Japanese, Scientific journal, GST融合タンパク質として作製したGlutathione-S-transferase融合タンパク質(sRANKL)はマクロファージ様細胞株RAW264.7(RAW)細胞を破骨細胞に分化させ,その濃度に依存して破骨細胞の数は増加した.sRANKLを加えて培養すると破骨細胞は一定期間は増加するが,その後にピークを迎えてから減少した.sRANKLはRAW細胞を破骨細胞に分化させると共に,破骨細胞にアポトーシスを誘導した
  • 機械的伸展刺激がマクロファージ様細胞のTNF-α産生に及ぼす影響
    李 紅雲; 出山義昭; 吉村善隆; 加藤晶久; 鈴木邦明; 飯田順一郎
    北海道歯学雑誌, 26, 2, 9, 2005, [Peer-reviewed]
    Japanese, Scientific journal
  • チロシンホスファターゼ阻害薬のNa+,K+-ATPaseに対する作用
    佐藤公哉; 鈴木邦明; 鎌田研祐; 出山義昭; 吉村善隆; 井上農夫男
    北海道歯学雑誌, 26, 200, 209, 2005, [Peer-reviewed]
    Japanese, Scientific journal
  • Na+,K+-ATPase活性のチロシンホスファターゼによる調節
    鎌田研祐; 出山義昭; 佐藤公哉; 吉村善隆; 鈴木邦明; 井上農夫男
    北海道歯学雑誌, 26, 190, 199, 2005, [Peer-reviewed]
    English, Scientific journal
  • Duration of alloantigen presentation and avidity of T cell antigen recognition correlate with immunodominance of CTL response to minor histocompatibility antigens
    Y Yoshimura; R Yadav; GJ Christianson; WU Ajayi; DC Roopenian; S Joyce
    JOURNAL OF IMMUNOLOGY, 172, 11, 6666, 6674, Jun. 2004, [Peer-reviewed], [Lead author]
    English, Scientific journal
  • 口腔扁平上皮癌によるインターロイキン-6の産生と顎骨浸潤との関連
    前川邦昭; 出山義昭; 吉村善隆; 八田光世; 鈴木邦明; 戸塚靖則
    北海道歯学雑誌, 25, 171, 178, 2004, [Peer-reviewed]
    Japanese, Scientific journal
  • Hydrostatic pressure stimulates the fusion process in Osteoclasts
    Yoshitaka Yoshimura; Takashi Kikuiri; Yoshiaki Deyama; Tomokazu Hasegawa; Mitsutoki Hatta; Kuniaki Suzuki
    Dentistry in Japan, 40, 35, 37, 2004, [Peer-reviewed], [Lead author, Corresponding author]
    English, Scientific journal
  • ヒト関節滑膜細胞に対する機械的伸展刺激が破骨細胞分化に及ぼす影響
    安東真毅; 出山義昭; 吉村善隆; 鈴木邦明; 飯田順一郎
    北海道歯学雑誌, 25, 35, 43, 2004, [Peer-reviewed]
    Japanese, Scientific journal
  • 破骨細胞分化に対するマクロファージ遊走阻止因子（MIF）の影響についてーマウス骨髄細胞ならびに骨芽細胞の共存培養条件下におけるMIFの作用ー
    田畑 太; 菊入 崇; 吉村善隆; 吉田英史; 白川哲夫; 小口春久
    小児歯科学雑誌, 41, 5, 860, 868, 2004, [Peer-reviewed]
    Japanese, Scientific journal
  • Another view of T cell antigen recognition: Cooperative engagement of glycolipid antigens by Va14Ja18 natural TCR
    AK Stanic; R Shashidharamurthy; JS Bezbradica; N Matsuki; Y Yoshimura; S Miyake; EY Choi; TD Schell; L Van Kaer; SS Tevethia; DC Roopenian; T Yamamura; S Joyce
    JOURNAL OF IMMUNOLOGY, 171, 9, 4539, 4551, Nov. 2003, [Peer-reviewed]
    English, Scientific journal
  • Chronic rejection of murine cardiac allografts discordant at the H13 minor histocompatibility antigen correlates with the generation of the H13-specific CD8(+) cytotoxic T cells
    JB Yang; A Jaramillo; W Liu; B Olack; Y Yoshimura; S Joyce; Z Kaleem; T Mohanakumar
    TRANSPLANTATION, 76, 1, 84, 91, Jul. 2003, [Peer-reviewed]
    English, Scientific journal
  • The H4(b) minor histocompatibility antigen is caused by a combination of genetically determined and posttranslational modifications
    R Yadav; Y Yoshimura; A Boesteanu; GJ Christianson; WU Ajayi; R Shashidharamurthy; AK Stanic; DC Roopenian; S Joyce
    JOURNAL OF IMMUNOLOGY, 170, 10, 5133, 5142, May 2003, [Peer-reviewed], [Lead author]
    English, Scientific journal
  • The effect of histamine on cyclooxygenase-2 induction in osteoblastic MC3T3-E1
    Yoshiaki Deyama; Yi-Gang Feng; Gen-ichi Ohnishi; Mitsutoki Hatta; Yoshitaka Yoshimura; Kuniaki Suzuki
    Dentistry in Japan, 39, 40, 42, 2003, [Peer-reviewed]
    English, Scientific journal
  • Expression of receptor activator of NF-kappa B ligand and osteoprotegerin in culture of human periodontal ligament cells
    T Hasegawa; Y Yoshimura; T Kikuiri; Y Yawaka; S Takeyama; A Matsumoto; H Oguchi; T Shirakawa
    JOURNAL OF PERIODONTAL RESEARCH, 37, 6, 405, 411, Dec. 2002, [Peer-reviewed]
    English, Scientific journal
  • Real-time T-cell profiling identifies H60 as a major minor histocompatibility antigen in murine graft-versus-host disease
    EY Choi; GJ Christianson; Y Yoshimura; N Jung; TJ Sproule; S Malarkannan; S Joyce; DC Roopenian
    BLOOD, 100, 13, 4259, 4265, Dec. 2002, [Peer-reviewed]
    English, Scientific journal
  • Immunodominance of H60 is caused by an abnormally high precursor T cell pool directed against its unique minor histocompatibility antigen peptide
    EY Choi; GJ Christianson; Y Yoshimura; TJ Sproule; NJ Jung; S Joyce; DC Roopenian
    IMMUNITY, 17, 5, 593, 603, Nov. 2002, [Peer-reviewed]
    English, Scientific journal
  • Histamine stimulates production of osteoclast differentiation factor/receptor activator of nuclear factor-kappa B ligand by osteoblasts
    Y Deyama; T Kikuiri; G Ohnishi; YG Feng; S Takeyama; M Hatta; Y Yoshimura; K Suzuki
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 298, 2, 240, 246, Oct. 2002, [Peer-reviewed]
    English, Scientific journal
  • Human periodontal ligament cells derived from deciduous teeth induce osteoclastogenesis in vitro
    T Hasegawa; T Kikuiri; S Takeyama; Y Yoshimura; M Mitome; H Oguchi; T Shirakawa
    TISSUE & CELL, 34, 1, 44, 51, Feb. 2002, [Peer-reviewed]
    English, Scientific journal
  • Regulation of alkaline phosphatase promoter activity by forkhead transcription factor FKHR
    M Hatta; H Daitoku; H Matsuzaki; Y Deyama; Y Yoshimura; K Suzuki; A Matsumoto; A Fukamizu
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 9, 2, 147, 152, Feb. 2002, [Peer-reviewed]
    English, Scientific journal
  • Involvement of Rb protein expression and phosphorylation in progression of oral carcinomas
    Yoshiaki Deyama; Toshiaki Ara; Motoaki Yasuda; Sadaaki Takeyama; Yoshitaka Yoshimura; Noriyuki Sakakibara; Kanchu Tei; Yasunori Totsuka; Kuniaki Suzuki
    Oral Therapeutics and Pharmacology, 21, 2, 59, 67, 2002, [Peer-reviewed]
    English, Scientific journal
  • Cytotoxic Effects of and Fluoride Release from All-in-one-step Dentinal Adhesives
    KAGA Masayuki; EDAHIRO Ayako; SAKURAI Makoto; OOKA Takafumi; NODA Mamoru; YOSHIMURA Yoshitaka; HIRONAKA Shouji; OGUCHI Haruhisa
    小児歯科学雑誌, 40, 4, 633, 640, 2002, [Peer-reviewed]
    Japanese, Scientific journal
  • Autoreactive diabetogenic T-cells in NOD mice can efficiently expand from a greatly reduced precursor pool
    DV Serreze; EA Johnson; HD Chapman; RT Graser; MP Marron; TP DiLorenzo; P Silveira; Y Yoshimura; SG Nathenson; S Joyce
    DIABETES, 50, 9, 1992, 2000, Sep. 2001, [Peer-reviewed]
    English, Scientific journal
  • Inactivation of NF-kappa B involved in osteoblast development through interleukin-6
    Y Deyama; S Takeyama; K Suzuki; Y Yoshimura; M Nishikata; A Matsumoto
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 282, 5, 1080, 1084, Apr. 2001, [Peer-reviewed]
    English, Scientific journal
  • Phosphate decreases osteoclastogenesis in coculture of osteoblast and bone marrow
    S Takeyama; Y Yoshimura; Y Deyama; Y Sugawara; H Fukuda; A Matsumoto
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 282, 3, 798, 802, Apr. 2001, [Peer-reviewed], [Corresponding author]
    English, Scientific journal
  • Quantitative analysis of the immune response to mouse non-MHC transplantation antigens in vivo: The H60 histocompatibility antigen dominates over all others
    EY Choi; Y Yoshimura; GJ Christianson; TJ Sproule; S Malarkannan; N Shastri; S Joyce; DC Roopenian
    JOURNAL OF IMMUNOLOGY, 166, 7, 4370, 4379, Apr. 2001, [Peer-reviewed]
    English, Scientific journal
  • 5, 5-diphenyl-2, 4-imidazolidinedione stimulates the expression of osteoclast differentiation factor (ODF/RANKL) by osteoblasts
    DEYAMA YOSHIAKI; TAKEYAMA SADAAKI; YOSHIMURA YOSHITAKA; NISHIKATA MAKOTO; SUZUKI KUNIAKI
    歯科薬物療法, 20, 3, 130, 136, 2001, [Peer-reviewed]
    Japanese, Scientific journal
  • Electron microscopic study of mineralized nodules formed in vitro by isolated fetal rat calvaria cells in absence of ascorbic acid and Na-beta-glycerophosphate
    Takanori Domon; Yoh Hisada; Yoshitaka Yoshimura; Akira Matsumoto; Shigeru Takahashi; Tsuneyuki Yamamoto; Minoru Wakita
    Dentistry in Japan, 37, 10, 15, 2001, [Peer-reviewed]
    English, Scientific journal
  • Low calcium environment effects osteoprotegerin ligand/osteoclast differentiation factor
    S Takeyama; Y Yoshimura; Y Shirai; Y Deyama; T Hasegawa; Y Yawaka; T Kikuiri; A Matsumoto; H Fukuda
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 276, 2, 524, 529, Sep. 2000, [Peer-reviewed], [Corresponding author]
    English, Scientific journal
  • Membrane type 1-matrix metalloproteinase expression is regulated by E-cadherin through the suppression of mitogen-activated protein kinase cascade
    T Ara; Y Deyama; Y Yoshimura; F Higashino; M Shindoh; A Matsumoto; H Fukuda
    CANCER LETTERS, 157, 2, 115, 121, Sep. 2000, [Peer-reviewed]
    English, Scientific journal
  • Osteoblast maturation suppressed osteoclastogenesis in coculture with bone marrow cells
    Y Deyama; S Takeyama; M Koshikawa; Y Shirai; Y Yoshimura; M Nishikata; K Suzuki; A Matsumoto
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 274, 1, 249, 254, Jul. 2000, [Peer-reviewed]
    English, Scientific journal
  • Cyclic tension force activates nitric oxide production in cultured human periodontal ligament cells
    T Kikuiri; T Hasegawa; Y Yoshimura; T Shirakawa; H Oguchi
    JOURNAL OF PERIODONTOLOGY, 71, 4, 533, 539, Apr. 2000, [Peer-reviewed]
    English, Scientific journal
  • Effect of extracellular calcium concentrations on osteoclast differentiation in vitro
    Y Shirai; Y Yoshimura; Y Yawaka; T Hasegawa; T Kikuiri; S Takeyama; A Matsumoto; H Oguchi
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 265, 2, 484, 488, Nov. 1999, [Peer-reviewed], [Corresponding author]
    English, Scientific journal
  • A phosphotyrosine-containing quenched fluorogenic peptide as a novel substrate for protein tyrosine phosphatases
    M Nishikata; K Suzuki; S Yoshimura; Y Deyama; A Matsumoto
    BIOCHEMICAL JOURNAL, 343, 2, 385, 391, Oct. 1999, [Peer-reviewed]
    English, Scientific journal
  • A low calcium environment enhances AP-1 transcription factor-mediated gene expression in the development of osteoblastic MC3T3-E1 cells
    A Deyama; Y Deyama; A Matsumoto; Y Yoshimura; M Nishikata; K Suzuki; Y Totsuka
    MINERAL AND ELECTROLYTE METABOLISM, 25, 3, 147, 160, May 1999, [Peer-reviewed]
    English, Scientific journal
  • Inhibition of Na+,K+-ATPase by cisplatin and its recovery by 2-mercaptoethanol in human squamous cell carcinoma cells
    N Sakakibara; K Suzuki; H Kaneta; Y Yoshimura; Y Deyama; A Matsumoto; H Fukuda
    ANTI-CANCER DRUGS, 10, 2, 203, 211, Feb. 1999, [Peer-reviewed]
    English, Scientific journal
  • Inhibition mechanism of Na,K-ATPase activity by local anesthetics and its reversibility
    K. Suzuki; I. Kawada; A. Iida; S. Ono; Y. Yoshimura; Y. Deyama; M. Nishikata; A. Matsumoto
    Oral Therapeutics and Pharmacology, 18, 2, 79, 83, 1999
    Japanese, Scientific journal
  • 局所麻酔薬によるNa, K-ATPase 活性の阻害機構と活性阻害の可逆性
    鈴木邦明; 川田 達; 飯田 彰; 小野智史; 吉村善隆; 出山義昭; 西方 眞; 松本 章
    歯科薬物療法, 18, 179, 183, 1999, [Peer-reviewed]
    English, Scientific journal
  • Cultured Human Periodontal Ligament Cells Produce Nitric Oxide Through the Interleukin-1 β-independent mechanism
    KIKUIRI Takashi; SHIRAKAWA Tetsuo; HASEGAWA Tomokazu; YOSHIMURA Yoshitaka; TAKEYAMA Sadaaki; KAGA Masayuki; OGUCHI Haruhisa
    小児歯科学雑誌, 37, 4, 768, 774, 1999, [Peer-reviewed]
    English, Scientific journal
  • Developmental profiles of phosphorylated and unphosphorylated CREBs in murine calvarial MC3T3-E1 cells
    MK Sakamoto; K Suzuki; S Takiya; Y Yoshimura; T Imai; A Matsumoto; S Nakamura
    JOURNAL OF BIOCHEMISTRY, 123, 3, 399, 407, Mar. 1998, [Peer-reviewed]
    English, Scientific journal
  • Epidermal growth factor receptor-mediated expression of NF-chi B transcription factor in osteoblastic MC3T3-E1 cells cultured under a low-calcium environment
    A Matsumoto; Y Deyama; A Deyama; M Okitsu; Y Yoshimura; K Suzuki
    LIFE SCIENCES, 62, 17-18, 1623, 1627, Mar. 1998, [Peer-reviewed]
    English, Scientific journal
  • Study on the Interaction between Gene Transcriptional Factors and Intercellular Signal Transduction in Osteoblast for the Prevention of Alveolar bone Collapse in Periodontal Disease
    MATSUMOTO Akira; KOBAYASHI Michiyo; DEYAMA Yoshiaki; YOSHIMURA Yoshitaka; SUZUKI Kuniaki; NAKAMURA Shinji; IKEDA Katsunmi; OHYA Keiichi
    日本歯科医学会誌, 17, 88, 93, 1998, [Peer-reviewed]
    Japanese, Scientific journal
  • Developmental profiles of cAMP response element binding activities in clonal murine calvarial MC3T3-E1 cells
    KOBAYASHI Michiyo; SUZUKI Kuniaki; TAKIYA Shigeharu; YOSHIMURA Yoshitaka; IMAI Tohru; MATSUMOTO Akira; NAKAMURA Shinji
    北海道歯誌, 19, 1, 80, 86, 1998, [Peer-reviewed]
    Japanese, Scientific journal
  • Inhibition of Na, K-ATPase activity by local anesthetics in rabbit brain and kidney and rat brain
    SUZUKI Kuniaki; KAWADA Itaru; SAKAKIBARA Noriyuki; KANETA Hiroyuki; YOSHIMURA Yoshitaka; DEYAMA Yoshiaki; NISHIKATA Makoto; MATSUMOTO Akira
    歯科薬物療法, 17, 3, 133, 137, 1998, [Peer-reviewed]
    Japanese, Scientific journal
  • Cytotoxic Effects of Composite Resin on Human Fibroblast-like Cells derived from Primary and Permanent Tooth Pulp through the Dentin Tubules in vitro
    HASEGAWA Tomokazu; KASHIWABARA Yoko; KIKUIRI Takashi; YOSHIMURA Yoshitaka; SHIRAKAWA Tetsuo; KAGA Masayuki; OGUCHI Haruhisa
    The Japanese Journal of Pediatric Dentistry, 36, 4, 646, 651, The Japanese Society of Pediatric Dentistry, 1998, [Peer-reviewed]
    Japanese, Scientific journal, Cytotoxic effects of unpolimerized composite resin which was given through the dentin tubules to the human pulp tissue were evaluated in vitro.
    Human fibroblast-Like cells(pulp cells) were isolated from extracted primary and permanent tooth pulp. The Bovine permanent tooth was cut into a dentin slice(thickness: 2 mm), and a cavity(diameter: 2 mm, depth: 1.7 m) was prepared in the slice. The cavity was filled with a composite resin (AP-X®), B-2, Kuraray Co. ) which was either light-polymerized or unpolimerized. Pulp cells on which the dentine slice containing composite resin was placed by using the cell-culture insert®(Falcon)were cultured for 24 hours.
    The number of surviving cells was counted after Trypan blue staining. In order to determine whether or not apoptosis occured in the cultured cells, fragmented DNA was extracted and electrophoresis was performed using 2% agarose gel. Concentration of IL-1β in the conditioned medium was determined by enzyme-linked immunosorbent assay(ELISA).
    The number of surviving cells decreased in both primary and permanent pulp cultures when the dentin slices filled with unpolimerized resin were applied. Polymerized resin had no cytotoxic effect on the cultured cells in the same culture condition. DNA fragmentation was not observed even when the unpolymerized resin affected the viability of the cells. IL-1β, which is often produced by inflamatory tissues, was detected in neither of the resin-treated cell cultures.
    These results indicated that unlike polymerized resin, unpolymerized resin components could pass through the dentin tubules and had cytotoxic effects on cultured cells. We conclude that the unpolymerized resin-induced cell death was apoptosis, as revealed null DNA fragmentation, but necrosis.
  • Effect of a low-calcium environment on EGF inducible AP-1 binding activity in osteoblastic MC3T3-E1 cells.
    A. Deyama; Y. Deyama; A. Matsumoto; M. Okitsu; Y. Yoshimura; K. Suzuki; Y. Totsuka
    Nucleic acids symposium series, 37, 193, 194, 1997
    English, Scientific journal
  • Effect of a low-calcium environment on alkaline phosphatase activity in embryonic rat calvarial bone cells in culture
    Y Yoshimura; Y Hisada; K Suzuki; Y Deyama; A Matsumoto
    ARCHIVES OF ORAL BIOLOGY, 41, 1, 41, 45, Jan. 1996, [Peer-reviewed], [Lead author, Corresponding author]
    English, Scientific journal
  • PHOSPHOTYROSYL PROTEIN PHOSPHATASE-LIKE ACTIVITY OF A CLONAL OSTEOBLASTIC CELL-LINE (MC3T3-E1 CELL)
    K SUZUKI; T OKAMOTO; Y YOSHIMURA; Y DEYAMA; Y HISADA; A MATSUMOTO
    ARCHIVES OF ORAL BIOLOGY, 40, 9, 825, 830, Sep. 1995, [Peer-reviewed]
    English, Scientific journal
  • The relation between nuclear gene expression and calcium ion in osteoblastic MC3T3-E1 cells.
    Y. Deyama; A. Matsumoto; Y. Yoshimura; K. Suzuki
    Nucleic acids symposium series, 34, 79, 80, 1995
    English, Scientific journal
  • The effect of calcium ion on the c-fos mRNA expression in the clonal osteoblastic MC3T3-E1 cells derived from mouse calvaria
    Yoshiaki Deyama; Akira Matsumoto; Yoshitaka Yoshimura; Kuniaki Suzuki
    ORAL THERAPEUTICS AND PHARMACOLOGY, 14, 2, 132, 137, 1995, [Peer-reviewed]
    English, Scientific journal
  • The relation between c-fos mRNA expression and protein kinase C activity in the MC3T3-E1 cells under a low calcium environment as a periodontal disease model
    Yoshiaki Deyama; Akira Matsumoto; Yoshitaka Yoshimura; Kuniaki Suzuki; Yoh Hisada
    ORAL THERAPEUTICS AND PHARMACOLOGY, 14, 1, 53, 58, 1995, [Peer-reviewed]
    English, Scientific journal
  • SENSITIVITY OF INTESTINAL ALKALINE-PHOSPHATASE TO L-HOMOARGININE AND ITS REGULATION BY SUBUNIT-SUBUNIT INTERACTION
    K SUZUKI; Y YOSHIMURA; Y HISADA; A MATSUMOTO
    JAPANESE JOURNAL OF PHARMACOLOGY, 64, 2, 97, 102, Feb. 1994, [Peer-reviewed]
    English, Scientific journal
  • C-FOS MESSENGER-RNA EXPRESSION AFTER TREATMENT WITH FETAL BOVINE SERUM AND EPIDERMAL GROWTH-FACTOR FOR OSTEOBLASTIC MC3T3-E1 CELLS CULTURED IN A LOW-CALCIUM ENVIRONMENT
    A MATSUMOTO; M OKITSU; Y DEYAMA; Y YOSHIMURA; K SUZUKI; Y HISADA; T UEMATSU
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 193, 1, 77, 87, May 1993, [Peer-reviewed]
    English, Scientific journal
  • The changes of c-fos mRNA expression in the protein kinase C activated-osteoblastic MC3T3-E1 cells cultured in a low-calcium environment.
    A. Matsumoto; Y. Deyama; M. Okitsu; Y. Yoshimura; K. Suzuki; Y. Hisada
    Nucleic acids symposium series, 29, 155, 156, 1993
    English, Scientific journal
  • The Change of DNA Synthesis Activity in the First Molar Tooth Germ of Neo-Natal Rat Cultured in Glow-Calcium Environment
    Akira Matsumoto; Yoh Hisada; Yoshitaka Yoshimura; Kuniaki Suzuki
    Oral Therapeutics and Pharmacology, 12, 2, 135, 139, 1993, [Peer-reviewed]
    English, Scientific journal
  • CHANGES IN PROTEIN-KINASE-C ACTIVITY IN RAT CALVARIAL BONE-CELLS CULTURED IN A LOW-CALCIUM ENVIRONMENT
    Y HISADA; A MATSUMOTO; Y YOSHIMURA
    ARCHIVES OF ORAL BIOLOGY, 37, 9, 695, 698, Sep. 1992, [Peer-reviewed]
    English, Scientific journal
  • Changes in intracellular free calcium concentrations after stimulation with concanavalin A or phospholipase C of bone cells cultured in low-calcium environments
    Yoh Hisada; Yoshitaka Yoshimura; Akira Matsumoto
    Dentistry in Japan, 282, 55, 61, 1991, [Peer-reviewed]
    English, Scientific journal
  • Calcium and phosphate concentrations, and alkaline and acid phosphatase activities in serum of the rat fed with low calcium and beryllium diets
    Akira Matsumoto; Yoh Hisada; Yoshitaka Yoshimura
    ORAL THERAPEUTICS AND PHARMACOLOGY, 10, 3, 253, 259, 1991, [Peer-reviewed]
    English, Scientific journal
■ Other Activities and Achievements
■ Lectures, oral presentations, etc.
  • メカニカルストレスが破骨細胞分化に与える影響
    吉村 善隆
    Feb. 2015, Japanese, Invited oral presentation
    [Invited], [Domestic Conference]
  • タイムラプス顕微鏡を用いた破骨細胞の観察
    吉村 善隆
    ニコンイメージングセンター開設一周年記念合同シンポジウム, 27 Oct. 2006, Japanese
    [Invited]
  • 抗原提示と認識の構造的特徴
    吉村 善隆
    北海道歯学会例会, 2004, Japanese
    [Invited]
  • 歯周病の歯槽骨崩壊予防のための骨芽細胞における遺伝子転写因子ならびに細胞内情報伝達系の相互制御機構の解明
    松本 章; 坂本美智代; 出山義昭; 吉村善隆; 鈴木邦明; 中村進治
    第12回歯科医学を中心とした総合的な研究を推進する集い, 1996, Japanese
    [Invited], [Domestic Conference]
■ Syllabus
  • 麻酔薬生体応答学研究, 2024年, 博士後期課程, 歯学院
  • 分子生物学的解析技法, 2024年, 博士後期課程, 歯学院
  • 歯学研究概論, 2024年, 博士後期課程, 歯学院
  • 細胞内情報伝達解析学研究, 2024年, 博士後期課程, 歯学院
  • 基本技法, 2024年, 博士後期課程, 歯学院
  • 有害・危険物質の取り扱いと管理, 2024年, 博士後期課程, 歯学院
  • 口腔病態解析学, 2024年, 博士後期課程, 歯学院
  • 薬理学・歯科薬理学Ⅰ, 2024年, 学士課程, 歯学部
  • 一般教育演習(ﾌﾚｯｼｭﾏﾝｾﾐﾅｰ), 2024年, 学士課程, 全学教育
  • 関連臨床医学, 2024年, 学士課程, 歯学部
  • 健康と社会, 2024年, 学士課程, 全学教育
  • アクティブラーニング科目Ⅳ, 2024年, 学士課程, 歯学部
  • 健康と社会, 2024年, 学士課程, 全学教育
  • 薬理学・歯科薬理学Ⅱ, 2024年, 学士課程, 歯学部
  • 薬理学・歯科薬理学実習, 2024年, 学士課程, 歯学部
■ Affiliated academic society
  • 北海道歯学会
  • 日本小児歯科学会
  • 日本歯科薬物療法学会
  • 歯科基礎医学会
  • 日本薬理学会
  • The International Association of Dental Research
■ Research Themes
  • 骨髄組織を伴う顎骨の再建法
    科学研究費助成事業
    Apr. 2025 - Mar. 2028
    吉村 善隆; 菊入 崇
    日本学術振興会, 基盤研究(C), 北海道大学, Principal investigator, 25K13241
  • 天然歯・人工歯の表面へのパターン付与治療へ向けた歯周組織再生の形態ロジック構築
    科学研究費助成事業
    01 Apr. 2023 - 31 Mar. 2027
    赤坂 司; 横山 敦郎; 吉村 善隆; 宮治 裕史; 牛島 夏未; 星 淡子
    日本学術振興会, 基盤研究(B), 北海道大学, 23H03733
  • 低酸素環境下での間葉系幹細胞のステムネス制御とエピジェネティクスの解明
    科学研究費助成事業
    Apr. 2024 - Mar. 2027
    菊入崇,吉村善隆,石山未紗
    日本学術振興会, 基盤研究(C), Coinvestigator, 24K13204
  • 間葉系幹細胞の骨芽細胞分化におけるメカノセンサー、メカノトランスダクションの解明
    科学研究費助成事業
    Apr. 2023 - Mar. 2026
    赤澤 友基; 吉村 善隆; 長谷川 智一
    細胞の分化に細胞外環境が重要であることはよく知られている。伸展力や圧迫力などの機械的刺激が骨芽細胞分化に影響を及ぼすことは多数報告されている。しかし、どの報告の力も生体内に換算すると大きな力で、わずかな力による報告は少ない。研究代表者らは間葉系幹細胞株UEF7-13に微小な圧迫力をかけることで、骨芽細胞に分化することを確認した。これは分化誘導因子を添加することなく石灰化を認めたことから、機械的刺激のみで成熟した骨芽細胞への誘導が可能なことを示唆している。本研究では、間葉系幹細胞の骨芽細胞への分化に関与するメカノセンサーの同定とその下流のメカノトランスダクションの解明、およびその臨床応用を目的とする。
    本年度の研究計画では、まず、間葉系幹細胞を分化させる至適圧迫力を探索することから開始した。予備実験で用いた圧迫力は0.005MPaであったが、圧迫力を0.0001MPa、0.0005MPa、0.001MPa、0.005MPa、0.01MPa、0.04MPaの幅で実験をする予定であった。しかし、0.0001MPa、0.0005MPa、0.001MPaのかなり微弱な圧迫力下で細胞を培養すると、細胞が死んでいく現象が続いた。培養液を頻回交換するなど検討を試みたが、圧迫力が弱いため、培養機内の空気交換が上手くできておらず細胞が酸欠状態に陥った可能性が考えられた。そこで、現在は培養機内の空気交換回数を上げるように設定しなおし、培養を継続している。現在のところ、細胞は死ぬことなく培養ができている。
    日本学術振興会, 基盤研究(C), 徳島大学, Coinvestigator, 23K09436
  • Mechanism for Epithelial-Mesenchymal Transition in Bone
    Grants-in-Aid for Scientific Research
    01 Apr. 2021 - 31 Mar. 2024
    田中 宗一; 吉村 善隆; 東野 史裕
    ビスホスホネート(BP)は、骨粗鬆症治療や癌の骨転移治療に用いられており、近年乳がんの転移や再発率の抑制など抗がん効果を有することが大規模臨床試験において示された。しかしながらBPは投与後速やかに尿中排泄され、生体内においてはほとんどが骨に蓄積し、破骨細胞を特異的に抑制することで骨吸収阻害効果を発揮することから、軟組織臓器において直接的な抗がん効果を発揮することは考えづらい。このことから、BPによるヒト乳がん患者の予後改善効果は、骨環境を抑制することで発揮されている可能性を推察した。骨には様々なサイトカインが豊富に含まれ骨吸収が生じるとそのサイトカインは骨髄中に放出されることが明らかになっているが、そのなかでもTGF-βは転移促進プログラムである上皮間葉転換(EMT)を誘導することが示されている。本研究においては、BPは骨吸収抑制作用によって骨髄中におけるがんのEMTを抑制する可能性について検討を進めた。まずEMTが骨髄中で促進されるか否かについてマウスモデルを作製し検討した。間葉細胞様形態を示さないヒト乳がん細胞MCF7を乳腺部皮下ならびに脛骨骨髄中に同時接種し腫瘍増大後EMT促進因子SnailのmRNA発現量を乳腺部腫瘍と比較しながら測定した。その結果、乳腺部腫瘍に比較して骨髄内腫瘍においてSnail発現が増加し、それに並行して上皮マーカーE-cadherinの発現が著明に減少した。この結果より骨髄はがん細胞のEMTに好都合な環境であることが推察された。更に現在はBPの培養細胞への添加がSnailをユビキチンプロテアソーム系での分解を促進するといった直接的なEMT抑制効果を有することを見いだしておりそのメカニズムを解析している。骨におけるがん細胞のEMTは現在まで示されておらず、本研究の進展は新たな組織特異的EMT制御メカニズムの一端を報告できると考えている。
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 21K10060
  • 閉経後の骨粗鬆症に対する乳歯由来歯髄幹細胞の培養上清の効果
    科学研究費助成事業
    01 Apr. 2020 - 31 Mar. 2023
    菊入 崇; 吉村 善隆
    本研究は、ヒト乳歯由来歯髄幹細胞から分泌されるサイトカインや成長因子、ケモカイン、エクソソームなどの生理活性分子群が骨組織のリモデリングにおいて骨吸収に対して抑制効果を有することを、動物実験によって明らかにすることを目的にしている。本年度の研究実績として、卵巣摘出後の骨粗鬆症モデルマウスを用い、ヒト乳歯由来歯髄幹細胞の培養上清液を投与し、エストロゲン欠乏によって発症する骨粗鬆症に対して抑制効果を有していたことについて報告を行った（Maeda A, et al., Exp Ther Med. 2022 Apr 23(4):299.）。
    この研究において、骨粗鬆症モデルマウスにヒト乳歯由来歯髄幹細胞の培養上清液を投与することで、エストロゲン欠乏による骨吸収が抑制されたことから、ヒト乳歯由来歯髄幹細胞の培養上清液には、骨リモデリングを正常化する因子が含まれていることが判明した。その作用機序についての詳細について検索を行ったところ、骨粗鬆症モデルマウスにおいてエストロゲン欠乏により活性化していた炎症性免疫細胞が、ヒト乳歯由来歯髄幹細胞の培養上清液の投与によってその活性が改善されていることを明らかにした。
    本成果と関連するデータにおいて、ヒト乳歯由来歯髄幹細胞の培養上清液中の生理活性分子群が免疫調節に関わることが予想されることから、ヒト乳歯由来歯髄幹細胞による免疫調整機能と骨組織のリモデリングにおける相互機能の解析が今後重要になると考えられた。
    日本学術振興会, 基盤研究(C), 20K10219
  • 超立体微細パターン付与だけで歯根膜再生を可能とするインプラント周囲炎治療技術開発
    科学研究費補助金(基盤研究(B))
    Apr. 2019 - Mar. 2023
    赤坂 司
    文部科学省, Competitive research funding
  • G-CSFは薬剤関連顎骨壊死を救えるのか？－マウスによる検討
    科学研究費補助金(基盤研究(C))
    Apr. 2019 - Mar. 2022
    吉村 善隆
    文部科学省, Principal investigator, Competitive research funding
  • 歯牙種発症に関しての研究
    科学研究費補助金(基盤研究(C))
    Apr. 2017 - Mar. 2020
    菊入 崇
    文部科学省, Competitive research funding
  • 歯根膜組織の再生・恒常性維持マシナリーに関与する新機能の探索および応用法の開発
    科学研究費補助金(基盤研究(C))
    Oct. 2016 - Mar. 2020
    長谷川智一
    文部科学省, Competitive research funding
  • 骨吸収抑制剤の休薬は顎骨壊死発症の予防に有効か？マウスによる検討
    科学研究費補助金(基盤研究(C))
    Apr. 2017 - Mar. 2019
    鄭 漢忠
    文部科学省, Competitive research funding
  • 造血因子によるMedication-related顎骨壊死治療の検討
    科学研究費補助金(基盤研究(C))
    Apr. 2016 - Mar. 2019
    吉村 善隆
    文部科学省, Principal investigator, Competitive research funding
  • 幹細胞のニッチに注目した顎骨再建法の開発
    科学研究費補助金(基盤研究(C))
    Apr. 2014 - Mar. 2017
    鄭 漢忠
    文部科学省, Competitive research funding
  • 乳歯歯髄由来間葉系幹細胞の骨芽細胞分化機転の解明
    科学研究費補助金(基盤研究(C))
    Apr. 2014 - Mar. 2017
    菊入 崇
    文部科学省, Competitive research funding
  • 歯周組織の恒常性維持機構における歯根膜機能の解析および新しい歯周組織再建法の開発
    科学研究費補助金(基盤研究(C))
    Apr. 2013 - Mar. 2016
    長谷川 智一
    文部科学省, Competitive research funding
  • アセチルサリチル酸によるＢＲＯＮＪ発症の予防法の開発
    科学研究費補助金(基盤研究(C))
    Apr. 2013 - Mar. 2016
    吉村 善隆
    文部科学省, Principal investigator, Competitive research funding
  • 顎裂部骨移植における幹細胞を用いた骨組織再生療法の試み―造血系幹細胞の併用―
    科学研究費補助金(基盤研究(C))
    Apr. 2012 - Mar. 2015
    松野 美乃
    文部科学省, Competitive research funding
  • 多発性歯牙腫を有している患者における原因因子の検索
    科学研究費補助金(挑戦的萌芽研究)
    Apr. 2012 - Mar. 2014
    菊入 崇
    文部科学省, Competitive research funding
  • 免疫担当細胞を標的とした新たなＢＲＯＮＪ治療法の開発
    科学研究費補助金(基盤研究(C))
    Apr. 2011 - Mar. 2014
    鄭 漢忠
    文部科学省, Competitive research funding
  • 重度歯周病罹患歯の残存歯根膜を根面に増殖させた歯とBMPの移植による歯周組織再生
    科学研究費補助金(基盤研究(C))
    Apr. 2010 - Mar. 2013
    齋藤 彰
    文部科学省, Competitive research funding
  • A development of the bonding treatment of vertically fractured roots with periodontal regeneration adhesive resin using tissue engineering
    Grants-in-Aid for Scientific Research
    2010 - 2012
    TANAKA Saori; SUGAYA Tsuomu; MIYAJI Hirofumi; SHIMOJI Shinnji; YOSHIMURA Yoshitaka; TANAKA Toru; OTANI Kaori; INOUE Kana; FUKUDA Hiroe; IBARA Asako; KANAYAMA Izumi; MOMOSE Takehito; NUSHIDA Erika; MIYATA Saori
    To enhance the bonding treatment of vertically fractured roots, we aimed to the newly cementum formation on a compound of resin and dentin powder, or β-TCP which may improve the binding between resin and cementum. In vitro, cytotoxicity was assessed. It had little cytotoxicity. In vivo, we observed the newly hard tissue formation on a resin containing β-TCP applied by rhBMP-2 and cultured bone marrow stromal cell sheets. This experiment might exhibit a possibility of the newly hard tissue formation on a resin.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 22592108
  • Analysis of pathological and/or unphysiological root resorption mechanism focused on the two types of osteoclasts
    Grants-in-Aid for Scientific Research
    2010 - 2012
    YOSHIMURA Yoshitaka; DOMON Takanori; KIKUIRI Takashi; DEYAMA Yoshiaki; HASEGAWA Tomokazu
    In this study, we examined the pathological and/or unphysiologicalroot resorption mechanism focused on the two types of osteoclasts. Our results weresuggested that morphologic characteristics of the osteoclasts varied according to thedown-regulation of Treg activity, difference of the major histocompatibility complex(MHC) haplotype, tensile force in culture condition and ROS scavengers.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Principal investigator, Competitive research funding, 22592274
  • Mechanisms of salivary gland atrophy induced by soft food
    Grants-in-Aid for Scientific Research
    2009 - 2012
    TAKAHASHI Shigeru; WAKITA Minoru; YAMAMOTO Tsuneyuki; FUNAHASHI Makoto; IIZUKA Tadashi; YOSHIMURA Yoshitaka
    Salivary glands of rats fed a liquid diet for 3 weeks were investigated microscopically to clarify the effects of soft food on salivary glands. Parotid glands of experimental rats became atrophic after liquid diet feeding, and cell shrinkage, cell death, and reduction of speed of cell-cycle were identified in the atrophic parotid glands. However, such atrophic alterations were not observed in submandibular and sublingual glands following liquid diet feeding. These observations suggest that it depends on the kind of salivary glands whether liquid diet feeding induces atrophy to the salivary glands.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Coinvestigator not use grants, Competitive research funding, 21390499
  • Study of evolution of osteoclasts regulating factors in teleost fish
    Grants-in-Aid for Scientific Research
    2009 - 2011
    DOMON Takanori; YOSHIMURA Yoshitaka
    The origin of osteoclasts resorbing bone remains unknown. Bone tissue is seen on teleost fish in phylogeny, and there old osteoclasts would be seen. The present study established the culture system of isolated Japanese medaka and observed their structure by light-and electron microscopy. The cultured cells showed no resorptive lacuna but etched surfaces on sperm whale dentine. These results may be related to the evolution of regulating fac tors of osteoclasts.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Coinvestigator not use grants, Competitive research funding, 21592319
  • Immunological approaches to the mechanisms of bisphosphonate-related osteonecrosis of the jaw
    Grants-in-Aid for Scientific Research
    2009 - 2011
    DEYAMA Yoshiaki; TOTSUKA Yasunori; YASUDA Motoaki; IIZUKA Tadashi; YOSHIMURA Yoshitaka
    In this study, we investigated the effects of bisphosphonate on osteoclasts and their precursor cells to clarify one of the mechanisms of bisphosphonate-related osteonecrosis of the jaw(BRONJ). BRONJ-related bisphosphonates strongly inhibited osteoclastogenesis and bone resorption activity. On the other hand, they showed cytotoxicity and enhanced microbial pathogens-induced cell death.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Coinvestigator not use grants, Competitive research funding, 21592511
  • The research for the niche factor of stem cells in culture condition
    Grants-in-Aid for Scientific Research
    2009 - 2011
    KIKUIRI Takashi; YOSHIMURA Yoshitaka
    Stem cells from human exfoliated deciduous teeth(SHED) are stem cell that differentiate into osteocyte and adipocyte. In this study, we investigated whether the Wnt signaling inhibitor has a potency of the stemness of SHED after long-term cell culture or not. We demonstrated that SHED treated with the Wnt signaling inhibitor was capable of stemness in vitro experiment and generating bone structures at 8 weeks post-transplantation into nude mouse. These data suggested that the Wnt signaling inhibitor acts as a stemness factor on SHED after long-term cell culture
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Coinvestigator not use grants, Competitive research funding, 21592584
  • Periodontal regeneration after implantation BMP and teeth with cells on the root planed surface populating from periodontal ligament in vitro
    Grants-in-Aid for Scientific Research(基盤研究(C))
    2007 - 2009
    Akira SAITO; Yoshitaka YOSHIMURA; Emiko SAITO
    The width of residual bone was one of the clinical host factors which affected bone regeneration following BMP-implantation. However, it did not affect connective tissue attachment, cementum regeneration and downgrowth of junctional epithelium. PDL cells that recognize and attach to dentin, don't form connective tissue attachment always. BMP also increased by the addition of connective tissue attachment may occur is suggested.
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(C), 北海道大学, Coinvestigator not use grants, Competitive research funding, 19592379
  • Analysis of the tooth eruption viaregulation of transcription factor
    Grants-in-Aid for Scientific Research(基盤研究(C))
    2007 - 2008
    Takashi KIKUIRI; Shohei OSHIMA; Yoshitaka YOSHIMURA
    本研究は、歯の発生の仕組みを明らかにするために、転写調節因子のひとつであるカルシニューリンについて着目し、歯胚形成期におけるカルシニューリンの発現について検索を行った。歯胚の分化期である蕾状期から鐘状期のではカルシニューリンは、歯性上皮由来の細胞、外胚葉性間葉由来の細胞両方において発現が確認された。以上のことからカルシニューリンは、歯の発生・分化過程において必要なシグナル分子として作用していることが示唆された。
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(C), 北海道大学, Coinvestigator not use grants, Competitive research funding, 19592344
  • Osteoimmunological analysis of pathological and/or unphysiologicalroot resorption in deciduous teeth involvement of T lymphocyte
    Grants-in-Aid for Scientific Research(基盤研究(C))
    2006 - 2008
    Yoshitaka YOSHIMURA; Haruhisa OGUCHI; Takanori DOMON; Yoshiaki DEYAMA
    本研究は、骨免疫学的観点からCD4 陽性T 細胞(Th1、Th2、Th17細胞)に着目し、病的あるいは非生理的な乳歯歯根吸収機構の解明を目的に検索を行った。Th1細胞の産生するインターフェロン-γ、Th2細胞の産生するインターロイキン(IL)-4は破歯細胞への分化を抑制し、Th17細胞の産生するIL-17は亢進した。これらの結果はCD4 陽性T 細胞が産生するサイトカインが歯根吸収機構に作用していることを示唆した。
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(C), 北海道大学, Principal investigator, Competitive research funding, 18592225
  • Study of odontotherapy rerated-osteonecrosis of the jaw following to long-term bisphosphonate application
    Grants-in-Aid for Scientific Research(基盤研究(C))
    2006 - 2007
    Yoshiaki DEYAMA; 戸塚 靖則; 鄭 漢忠; 飯塚 正; 吉村 善隆
    Intravenous bisphosphonates are primarily used and effective in the treatment and management of cancer-related conditions. These include hypercalcemia of malignancy, skeletal-related events associated with bone metastases in the context of solid tumors such as breast cancer, prostate cancer, and lung cancer, and in the management of lytic lesions in the setting of multiple myeloma. Oral bisphosphonates are approved to treat osteoporosis and are frequently used to treat osteopenia as well. They are also used for a variety of less common conditions such as Paget's disease of bone, and osteoge...
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(C), 北海道大学, Coinvestigator not use grants, Competitive research funding, 18592162
  • The analysis of the network transcription regulation by the IKK・r in the tooth differentiation and development.
    Grants-in-Aid for Scientific Research(基盤研究(C))
    2005 - 2006
    Takashi KIKUIRI; 長谷川 智一; 吉村 善隆
    The tooth development is adjusted by the appearance of various genes and growth factors such as cytokines, and a complex signal network is clarified to the differentiation and the formation of teeth. However, details of all mechanisms in the tooth development are not clarified. In this research, we forced the IKK-y signaling in the mechanism of the teeth development.1. Confirmation of mRNA appearance of IKK-γ in the early stage of teeth development The tooth germ of each stage and the surrounding tissue were removed from mouse's jaw bone and cultured. We did the in situ hybridization of IKK...
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(C), 北海道大学, Coinvestigator not use grants, Competitive research funding, 17592125
  • Development of a new periodontal regeneration technique for the teeth with reduced periodontal ligament using the combination of tissue culture and transplantation of the teeth
    Grants-in-Aid for Scientific Research(基盤研究(C))
    2005 - 2006
    Akira SAITO; 吉村 善隆; 齋藤 恵美子
    It has been suggested that cells from the periodontal ligament have the potential to create new connective tissue attachment. The periodontal ligament cells populated limitedly on the root surface of the wound in vivo due to epithelium down growth. We have been developing a new periodontal regeneration technique for the teeth with reduced periodontal ligament using the combination of tissue culture and transplantation of the teeth.The purpose of this study was to examine effects of enamel matrix derivative (EMD) application on periodontal healing after implantation of teeth with cells popul...
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(C), 北海道大学, Coinvestigator not use grants, Competitive research funding, 17592157
  • RNAiを用いた自然免疫系制御による硬化性骨髄炎の治療戦略
    科学研究費補助金(萌芽研究)
    2005 - 2006
    鄭 漢忠; 戸塚 靖則; 出山 義昭; 吉村 善隆
    慢性骨髄炎などの骨硬化を伴う疾患においては病巣局所に対する薬物の移行性が低下しており、治療上大きな障害となっている。骨硬化の原因としては局所に存在する骨芽細胞の機能が亢進し、石灰化が促進していることが推測される。病原体の侵入を特異的に認識することが知られているToll様受容体(TLR)がマクロファージ等の破骨細胞前駆細胞に発現しており、細菌性抗原などを認識して破骨細胞に分化することが知られている。本研究において、われわれは骨芽細胞におけるTLRを介したシグナルに関して研究を行い、それらの関与する遺伝子発現を制御することにより、硬化性骨髄炎の治療に応用することを試みようとした。石灰化現象を観察可能な骨芽細胞としてMC3T3-E1細胞を用い、硬化性骨髄炎の原因の一つであると推測されるウイルスの2本鎖RNAの代用としてpoly(I):poly(C)を用いて実験を行った。RT-PCR法ならびにウエスタンブロット法によりMC3T3-E1細胞には2本鎖RNAを認識するTLR3が発現していた。MC3T3-E1細胞をpoly(I):poly(C)処理すると転写因子STAT1の活性化が生じ、インターフェロン(IFN)β、CXCL10、TLR3の発現が増加した。これらの発現の増加は抗IFN-β抗体を細胞に作用させることにより抑制された。これらの結果からウイルス感染を生ずるとTLR3の発現を増加さ...
    文部科学省, 萌芽研究, 北海道大学, Coinvestigator not use grants, Competitive research funding, 17659624
  • 各種MHCクラスI/ペプチド複合体とT細胞レセプターとの反応速度論的解析
    2005 - 2005
    吉村 善隆
    公益信託 西宮機能系基礎医学研究助成基金, Principal investigator, Competitive research funding
  • Mechanism of condensing osteitis crisis following viral infection through toll-like receptors
    Grants-in-Aid for Scientific Research(基盤研究(C))
    2004 - 2005
    Yoshiaki DEYAMA; 戸塚 靖則; 鈴木 邦明; 柴田 健一郎; 土門 卓文; 吉村 善隆
    The aim of this project is to clarify the mechanisms of condensing osteitis crisis concerning to viral infection. Toll-like receptors (TLR) 1,3-7 expressed in osteoblastic MC3T3-E1 (E1) cells, TLR 1-7 in stromal ST2 cells, and TLR 3,6, and 7 in osteosarcoma cell, MG-63. TLR-3 expression is maximal in all of cells. When these cells were grown to confluent, and then treated with poly I:C for more two weeks, they formed mineralized nodules. It seems to be earlier than in control culture. DNA transfection experiments in with poly I:C treated-MG-63 cells were performed with reporter gene constru...
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(C), 北海道大学, Coinvestigator not use grants, Competitive research funding, 16591880
  • 骨再生を目指した転写因子Osterixの機能調節機構の解明
    科学研究費補助金(萌芽研究)
    2004 - 2005
    加我 正行; 吉村 善隆; 出山 義昭
    昨年度に引き続き、Osterix転写活性化領域の詳細を同定するために、GAL4-binding sitesと連接させたルシフェラーゼreporter plasmidを用いたGal4融合システムレポーター分析を行い、Osterixの転写活性を調べた。その結果、Osterixの転写活性領域がプロリンとグリシン残基に富んでおり、哺乳類とイーストの細胞における活性の特性を持っていることを認めた。GST-pull down分析および免疫沈降法では、TBPではなく、基本転写因子であるTF-IIBが転写活性領域に結合することを示した。さらに、Osterix相互作用因子を探索するために、FLAG-tagを付加したOsterix発現plasmidを培養細胞に導入し、αFLAG抗体を用いた免疫沈降法を行ったところ、in vivoとin vitroにおいて、OsterixがそのC末端Zinc-Finger領域を通して、クロマチンリモデリング因子Brg-1および癌抑制遺伝子p53と相互作用することを見つけた。Osterixにより発現制御される遺伝子群の検索するために、G418耐性遺伝子を有したOsterix発現plasmidを作製し、薬剤選択によりOsterix遺伝子をstableに過剰発現するSaOS2細胞を樹立し、その遺伝子発現について解析した。RT-PCR法により骨芽細胞特異的遺伝子群の発現に...
    文部科学省, 萌芽研究, 北海道大学, Coinvestigator not use grants, Competitive research funding, 16659567
  • 移植免疫における副組織適合抗原認識機構の解明
    研究開発助成事業 基盤的研究開発育成事業 若手研究補助金
    2004 - 2004
    吉村 善隆
    財団法人 北海道科学技術総合振興センター（ノーステック財団）, Principal investigator, Competitive research funding
  • 乳歯歯根吸収の制御を目的とした遺伝子治療のための基礎的研究
    科学研究費補助金(若手研究(B))
    2002 - 2004
    吉村 善隆
    本年度はreceptor activator of NF-kappa B (RANK) ligand (RANKL)の真核細胞発現ベクターをマウス骨髄由来細胞ならびにマウス由来株化細胞(RAW264.7細胞、J774-1細胞)への遺伝子導入を行った。インデューサ(テトラサイクリン)を添加時にのみ目的遺伝子を発現するベクターを用いることによって、目的タンパク質の発現がコントロール可能となり、適切な時に適切な発現を可能とした。しかしながら、リポフェクション法、エレクトロポレーション法では、ともに導入率が0.01%以下であった。また、レトロウイルスベクターを用いた方法では導入率が0.01%程度であった。以上のように、遺伝子導入を効果的・効率的に行うことができなかった。予備実験の結果から炎症性サイトカインの1つであるマクロファージ遊走阻止因子(MIF)はその濃度に依存して、TRAP陽性細胞の総核数を変化させることなく、マウス骨髄由来細胞から分化したTRAP陽性多核細胞の出現数を減少させることが判明した。そこで、MIFの真核細胞発現ベクターをマウス骨髄由来細胞ならびにマウス由来株化細胞への遺伝子導入を、上記と同様にリポフェクション法、エレクトロポレーション法、レトロウイルスベクターを用いた方法で行ったが、効果的・効率的に行うことができなかった。マウス由来株化細胞(RAW264.7細胞)...
    文部科学省, 若手研究(B), 北海道大学, Principal investigator, Competitive research funding, 14771172
  • The reaction mechanism of general anesthetics studied by electron spin resonance and fluorescence measurement technique
    Grants-in-Aid for Scientific Research(基盤研究(B))
    2002 - 2004
    Kuniaki SUZUKI; 吉村 善隆; 出山 義昭; 平沖 敏文; 飯田 彰; 福島 和昭
    We investigated the effects of general anesthetics and the temperature on electron spin resonance (ESR) spectra of multilamellar liposome (MLV). Liposomes were composed of DMPC, DOPC or EYPC, labeled with 5-DSA or 16-DSA. The ESR spectra were measured at different temperatures from 10 to 50 ℃. The spectra of MLV labeled by 16-DSA showed three sharp signals compared with 5-DSA. The difference of order parameter S value of 5-DSA and 16-DSA suggested that the nitroxide radical location of 5-DSA and 16-DSA were different in the membrane bilayr. The anesthetics did not alter the S and τ values, ...
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 14370653
  • MHCクラスI/副組織適合抗原ペプチド複合体とTCRとの分子間相互作用の解析
    秋山記念生命科学研究助成金＜奨励助成＞
    2003 - 2003
    吉村 善隆
    財団法人秋山記念生命科学振興財団, Principal investigator, Competitive research funding
  • 遺伝子治療による新しい歯根吸収制御法の開発
    科学研究費補助金(萌芽研究)
    2003 - 2003
    小口 春久; 吉村 善隆; 八若 保孝; 加我 正行
    骨形成・骨吸収の遺伝子治療が考えられている現在、病的あるいは非生理的な「歯根吸収の制御」を遺伝子治療という観点から考えることは、新しい研究分野の展開につながる将来性のある研究の第一歩と考える。破骨細胞は骨吸収を担う多核細胞であり、単球・マクロファージ系細胞の前駆細胞から分化することが知られている。予備実験の結果から、今回我々は、炎症性サイトカインの1つであるマクロファージ遊走阻止因子(MIF)が、破骨細胞分化過程にどのような影響を及ぼすかについて検討を行った。マウス骨芽細胞様細胞(MC3T3-E1細胞)とマウス骨髄細胞との共存培養系によって破骨細胞を誘導し、酒石酸耐性酸性ホスファターゼ(TRAP)活性陽性細胞の出現数、破骨細胞分化因子(RANKL)の発現量、破骨細胞抑制因子(OPG)の産生量および象牙質切片を用いた硬組織吸収能についてMIFの影響を調べた。その結果、TRAP陽性多核細胞の出現数は、MIF濃度に依存して減少することが判明した。一方、MC3T3-E1細胞におけるRANKLの発現およびOPGの産生に、MIFの影響は確認されなかった。TRAP陽性細胞1個中に含まれる核数の分布を調べたところ、MIF作用群ではコントロールと比較し、多核化が抑制されていることが明らかになった。また、象牙質切片上に形成された吸収窩を観察したところ、MIF作用群では形成された吸収窩の大きさが明...
    文部科学省, 萌芽研究, 北海道大学, Coinvestigator not use grants, Competitive research funding, 15659489
  • The identification of genes associated with the root resorption of deciduous tooth and molecular biology analysis of control mechanism.
    Grants-in-Aid for Scientific Research(基盤研究(C))
    2002 - 2003
    長谷川 智一; masato MITOME; 菊入 崇; 吉村 善隆
    We have been analyzed the periodontal cells, in order to clarify the mechanism of the root resorption. As part of this study, some genes specifically discovered in the periodontal cell were identified. The macrophage inhibit migration factor (MIF) is one of the genes which identified.MIF has been characterized as a pro-inflammatory cytokine. MIF not only plays its role in immune system but is also involved in cell proliferation and differentiation in a variety of organs. There is no report demonstrating the effect of MIF on osteoclast formation. We investigated the regulatory effects of mac...
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(C), 北海道大学, Coinvestigator not use grants, Competitive research funding, 14571933
  • Interaction of osteoclasts and cancer cells in the process of bone invation of oral cancer
    Grants-in-Aid for Scientific Research(基盤研究(B))
    2000 - 2002
    Kanchu TEI; 出山 義昭; 長谷川 智一; 土門 卓文; 進藤 正信; 吉村 善隆; 戸塚 靖則; 出山 義昭
    Objectives; Osteoclastic bone resorption is an important step in bone invasion in oral squamous cell carcinomas (SCC). Although several cytokines accelerates osteoclastic bone resorption, it remains unclear whether oral SCC conditioned media may be involved in osteoclastogenesis.Methods: The co-culture system using osteoblasts and bone marrow cells was performed to examine the activity of osteoclastogenesis by oral cancer cells. Conditioned medium (CM) from oral cancer cells and/or anti-interleukin-6 (IL-6) antibody were added into co-culture. We then used a neutralizing anti-body against o...
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 12470432
  • Approach to reaction mechanism of general anesthetics by biological and physicochemical methods from new viewpoint
    Grants-in-Aid for Scientific Research(基盤研究(B))
    1999 - 2001
    Kuniaki SUZUKI; 吉村 喜隆; 出山 義昭; 平沖 敏文; 飯田 彰; 吉村 善隆
    l) We studied the effects of volatile anesthetics, benzodiazepines, ketamine and droperidol on Na^+, K^+-ATPase activity; (1) All of anesthetics except fentanyl inhibited Na, K-ATPase activity and its partial reactions in a dose dependent manner. There were certain patterns, characteristic of each category of the anesthetics, in the order of IC50 values of Na, K-ATPase activity and its partial reactions. Effects of the anesthetics on the phosphointermediate (EP) formation differed among the anesthetics. Benzodiazepine inhibited the EP formation, whereas isoflurane and ketamine did not. The ...
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 11470428
  • 新しい視点からの麻酔薬の作用および副作用の機序の解明と新規麻酔薬評価への応用
    科学研究費補助金(萌芽的研究)
    1997 - 1998
    鈴木 邦明; 出山 義昭; 吉村 善隆
    ウサギ脳Na^+,K^+-AIPaseを用いて、種々の全身麻酔薬およびその関連薬がNa^+,K^+-AIPaseに及ぼす影響と作用機構について調べた。その結果、フェンタニルを除くすべての麻酔薬および関連薬は、濃度依存性にNa^+,K^+-ATPase全活性と部分反応であるNa^+-ATPase活性(Na^+活性)およびK^+-pNPPase(K^+活性)を阻害した。揮発性麻酔薬においては50%阻害濃度(IC_<50>値)と一般に麻酔作用の強さの指標とされるMAC値の間に強い相関関係(r=0.938)がみられた。各麻酔薬の全活性、 Na^+活性およびK^+活性に対するIC_<50>値の順序を比較したところ、バルビタール類、ペンゾジアゼピン類、揮発性麻酔薬およびその他の麻酔薬で順序のパターンに差異がみられ、麻酔薬のグループごとに阻害機構が異なることが示唆された。そこで各麻酔薬のリン酸化反応中間体(EP)形成に対する作用を調べたところ、全活性が阻害される濃度でベンゾジアゼピン類はEP形成を抑制し、揮発麻酔薬とケタミンは抑制しなかった。ミダゾラム、イソフルラン、ケタミンの濃度を変えてEP形成に対する作用を調べたところペンゾジアゼピン類であるミダゾラムのみが濃度依存性にEP形成を抑制した。また、揮発性麻酔薬であるイソフルランにおいてEPのK^+に対する反応性は低下し、ケタミンでは増加し...
    文部科学省, 萌芽的研究, 北海道大学, Coinvestigator not use grants, Competitive research funding, 09877298
  • 口腔癌発癌過程における転写因子RB蛋白質の関与に関する研究
    科学研究費補助金(基盤研究(C))
    1996 - 1998
    鄭 漢忠; 吉村 喜隆; 出山 義昭
    癌抑制遺伝の一つであるRbタンパク質(pRb)は転写因子E2Fと結合することにより細胞分裂を抑制しており、pRbがサイクリン-サイクリン依存性キナーゼ複合体によってリン酸化されるとE2Fと離開し、細胞分裂が開始することが知られている。これまでにpRbの発現の低下および遺伝子の変異が種々の癌で報告されているが、そのリン酸化の程度を調べた研究はない。今回我々は口腔癌20症例を対象としてpRbの発現およびそのリン酸化の状態をリン酸化部位特異的抗Rb抗体を用いて検索した。対照群6例では全症例でpRbを発現していたのに対し、腫瘍群では20例中9例でのみpRbを発現していた。また、pRb発現症例に限定すると対照群のpRbはリン酸化されていなかったのに対し、腫瘍群ではThr373のリン酸化が5症例、Ser780は6症例、Ser807/Ser811は8症例で生じていた。リン酸化の程度はどの部位においてもT1からT4になるにつれて上昇する傾向が見られた。pRbを発現しかつ低リン酸化型の症例を活性化型、pRbを喪失しているか高リン酸化型の症例を不活性化型として分類すると、対照群は6症例とも活性化型として、腫瘍群はThr373では20症例中16症例(80%)、Ser780では17症例(85%)、Ser807/Ser811では19症例(95%)が不活性型として分類され、対照群と腫瘍群間で有意差が認め...
    文部科学省, 基盤研究(C), 北海道大学, Competitive research funding, 08672279
  • Study on transcription factor NF-kB regulating the gene function of tyrosin kinase in osteoblast
    Grants-in-Aid for Scientific Research(一般研究(B), 基盤研究(B))
    1995 - 1997
    Akitra MATSUMOTO; 吉村 善隆; 鈴木 邦明
    We examined about the interaction between gene transcription factors and intracellular signal transduction, using the osteoblast-rich bone cells and osteoblastic Mc3T3-E1 cells(E1 cells)cultured under a low-calcium environment for the prevention of alveolarbone collapse seen in the patients with periodontal disease.In the bone cells cultured in a low-calcium medium, intracellular calcium content and free calcium concetration significantly decreased, compared with those in normal one (p<0.01)It was found that the binding sites of NF-kB,CREB and Oct-1 of gene transcription factors existed in ...
    Ministry of Education, Culture, Sports, Science and Technology, 一般研究(B), 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 07457436
  • 骨形成細胞の石炭化におけるアルカリ性ホスファターゼの生理的役割の解明-異なる培養条件を用いて-
    科学研究費補助金(奨励研究(A))
    1994 - 1994
    吉村 善隆
    ラット胎児頭蓋冠由来骨形成細胞をBGJ_b並びにαMEMを用い培養し、これら細胞のアルカリ性ホスファターゼ(ALP)活性を測定した。活性は、細胞が単層期(con fluence)では正常環境(対照)に比べ、低カルシウム濃度培養液(低Ca環境)で有意に高い値を示した。この活性は、細胞多層化期(multilayer)では両培養液の細胞共に対照では上昇したが、低Ca環境では変動せず、その結果として対照と低Ca環境下の細胞間で、有意な差は認められなくなった。活性は、その後では対照における活性はBGJ_bで上昇せず、αMEMで上昇を続けた。光学並びに電子顕微鏡に認められた石炭化部位は、BGJ_bの対照並びに両培養液の低Ca環境では認められず、αMEMの対照のみ認められた。αMEMの対照ではALP活性の上昇並びに細胞の多層化と共に結節を形成し、石炭化の開始が認められた。オステオカルシンはαMEMの対照のみに検出された。これらの結果は、多くの研究で行われているような培養液へのβ-glycerophosphateやdexamethasone等の添加をすることなしに得られた。以上の結果から、低Ca培養液において細胞のALP活性が亢進することは細胞の機能を考える上で興味深く、その一つとして環境の異常に対する細胞の反応が推測された。また、培養液の違いによる石炭化現象の有無は、BGJ_bとαMEMの...
    文部科学省, 奨励研究(A), 北海道大学, Principal investigator, Competitive research funding, 06771619
  • Study on the function and regulation of gene coding tyrosine kinase
    Grants-in-Aid for Scientific Research(一般研究(B))
    1992 - 1994
    松本 章; Akira MATSUMOTO; 吉村 善隆; 鈴木 邦明; 久田 洋
    It was examined whether the gene function of mouse osteoblastic MC3T3 - E 1 Cells (E 1 cells) was affected by a low-calcium environment.In the E 1 cells with the treatment of FBS or EGF,c-fos mRNA expression increased with time in the control and low Ca groups, reached maximum at 30 minutes and then decreased at 60 minutes. The degree of the expression was significantly higher in low Ca group than in control group (p<0.01).Since EGF activates tyrosine kinase of receptor type, it was speculated that a tyrosine kinase is involved in this enhancement in low Ca group.When the E1 cells was pretr...
    Ministry of Education, Culture, Sports, Science and Technology, 一般研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 04454462
  • Establishment of a osteoblastic cell line derived from calvarial of rat embryo.
    Grants-in-Aid for Scientific Research(一般研究(C))
    1992 - 1994
    Yoh HISADA; 吉村 善隆
    An osteoblastic cell line that retains the capacity to mineralize in vitro would be a very useful model system for the study of the mechanism of calcification.However a few lines have established including the origin of cancer cells.The present study aimed to establish the cell line from calvariae of 18-21-old rat embryo.Isolation of osteoblastic cells : (1) Single-cell suspensions from enzymatic digestions of the calvariae.(2) Outgrowth from the flagments of calvariae.Culture medium : alpha-MEM with 10% FBS AND 60mug/ml Kanamycine sulfate.The changes of media at every 2 days.Subculture at ...
    Ministry of Education, Culture, Sports, Science and Technology, 一般研究(C), 北海道大学, Coinvestigator not use grants, Competitive research funding, 04671117
  • 石灰化過程及び低カルシウム環境下でのアルカリ性ホスファターゼの生理的役割の解明
    科学研究費補助金(奨励研究(A))
    1993 - 1993
    吉村 善隆
    ラット胎児頭蓋冠由来骨形成細胞を、BGJb並びにalphaMEMを用い培養した。これら細胞のアルカリ性ホスファターゼ(ALP)活性は、細胞が単層の時期では正常環境(対照)に比べ、低カルシウム濃度培養液(低Ca環境)で有意に高い値が認められた。細胞が多層化する時期では、この活性は両培養液の細胞共に対照では上昇したが、低Ca環境では変動せず、その結果として対照と低Ca環境下の細胞間で、有意な差が認められなくなった。その後、対照における活性はBGJbでは上昇せず、alphaMEMでは上昇を続けた。石灰化部位は、BGJbの対照並びに両培養液の低Ca環境では認められず、dMEMの対照のみ認められた。dMEMの対照はALP活性の上昇並びに細胞の多層化と共に結節を形成し、石灰化の開始が認められた。引き続き、結節間の連結が生じることも認められた。走査型電子顕微鏡像では細胞膜には多数の長い突起が認められ、伸展した突起の一部は他の細胞と結合していた。また、透過型電子顕微鏡像ではコラーゲン線維と基質小胞が認められた。オステオカルシンはalphaMEMの対照のみに検出された。これらの結果は、beta-glycerophosphateやdexamethasone等を用いることなく得られた。一方、BGJbを用いた場合、本研究の初代培養細胞のみならず骨芽細胞様細胞株(MC3T3-E1細胞)でもALP活性の...
    文部科学省, 奨励研究(A), 北海道大学, Principal investigator, Competitive research funding, 05771506
  • 低カルシウム環境下における骨由来細胞のアルカリ性ホスファターゼの生理的役割の解明
    科学研究費助成事業
    1992 - 1992
    吉村 善隆
    日本学術振興会, 奨励研究(A), 北海道大学, 04771445
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