Researcher basic information

• 博士（理学）, 北海道大学

• https://researchmap.jp/read0167122?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200901063813109506
• https://noah.ees.hokudai.ac.jp/yoshida/

• 200901063813109506

• 微生物学
• 発生遺伝学
• 分子遺伝学
• Molecular Genetics Developmental Genetics

• Life Science, Applied microbiology
• Life Science, Developmental biology
• Life Science, Genetics

• Bachelor's degree program, School of Science
• Master's degree program, Graduate School of Environmental Science
• Doctoral (PhD) degree program, Graduate School of Environmental Science

Research activity information

• Effects of Aerobic Growth on the Fatty Acid and Hydrocarbon Compositions of Geobacter bemidjiensis Bem(T)
  Akio Ueno; Satoru Shimizu; Mikako Hashimoto; Takumi Adachi; Takako Matsushita; Hidetoshi Okuyama; Kiyohito Yoshida
  JOURNAL OF OLEO SCIENCE, 66, 1, 93, 101, Jan. 2017, [Peer-reviewed]
  English, Scientific journal
• Bacterial Long-Chain Polyunsaturated Fatty Acids: Their Biosynthetic Genes, Functions, and Practical Use
  Kiyohito Yoshida; Mikako Hashimoto; Ryuji Hori; Takumi Adachi; Hidetoshi Okuyama; Yoshitake Orikasa; Tadashi Nagamine; Satoru Shimizu; Akio Ueno; Naoki Morita
  MARINE DRUGS, 14, 5, May 2016, [Peer-reviewed]
  English
• Occurrence of trans monounsaturated and polyunsaturated fatty acids in Colwellia psychrerythraea strain 34H
  Mikako Hashimoto; Yoshitake Orikasa; Hidenori Hayashi; Kentaro Watanabe; Kiyohito Yoshida; Hidetoshi Okuyama
  JOURNAL OF BASIC MICROBIOLOGY, 55, 7, 838, 845, Jul. 2015, [Peer-reviewed]
  English, Scientific journal
• Structural Confirmation of a Unique Carotenoid Lactoside, P457, in Symbiodinium sp Strain nbrc 104787 Isolated from a Sea Anemone and its Distribution in Dinoflagellates and Various Marine Organisms
  Takahiro Wakahama; Aitor Laza-Martinez; Ahmad Iskandar Bin Haji Mohd Taha; Hidetoshi Okuyama; Kiyohito Yoshida; Kazuhiro Kogame; Koichiro Awai; Masanobu Kawachi; Takashi Maoka; Shinichi Takaichi
  JOURNAL OF PHYCOLOGY, 48, 6, 1392, 1402, Dec. 2012, [Peer-reviewed]
  English, Scientific journal
• Polytene chromosomal maps of 11 Drosophila species: The order of genomic scaffolds inferred from genetic and physical maps
  Stephen W. Schaeffer; A. Un Bhutkar; Bryant F. McAllister; Muneo Matsuda; Luciano M. Matzkin; Patrick M. O'Grady; Claudia Rohde; Vera L. S. Valente; Montserrat Aguade; Wyatt W. Anderson; Kevin Edwards; Ana C. L. Garcia; Josh Goodman; James Hartigan; Eiko Kataoka; Richard T. Lapoint; Elena R. Lozovsky; Carlos A. Machado; Mohamed A. F. Noor; Montserrat Papaceit; Laura K. Reed; Stephen Richards; Tania T. Rieger; Susan M. Russo; Hajime Sato; Carmen Segarra; Douglas R. Smith; Temple F. Smith; Victor Strelets; Yoshiko N. Tobari; Yoshihiko Tomimura; Marvin Wasserman; Thomas Watts; Robert Wilson; Kiyohito Yoshida; Therese A. Markow; William M. Gelbartt; Thomas C. Kaufman
  GENETICS, 179, 3, 1601, 1655, Jul. 2008
  English
• Evolution of genes and genomes on the Drosophila phylogeny
  Andrew G. Clark; Michael B. Eisen; Douglas R. Smith; Casey M. Bergman; Brian Oliver; Therese A. Markow; Thomas C. Kaufman; Manolis Kellis; William Gelbart; Venky N. Iyer; Daniel A. Pollard; Timothy B. Sackton; Amanda M. Larracuente; Nadia D. Singh; Jose P. Abad; Dawn N. Abt; Boris Adryan; Montserrat Aguade; Hiroshi Akashi; Wyatt W. Anderson; Charles F. Aquadro; David H. Ardell; Roman Arguello; Carlo G. Artieri; Daniel A. Barbash; Daniel Barker; Paolo Barsanti; Phil Batterham; Serafim Batzoglou; Dave Begun; Arjun Bhutkar; Enrico Blanco; Stephanie A. Bosak; Robert K. Bradley; Adrianne D. Brand; Michael R. Brent; Angela N. Brooks; Randall H. Brown; Roger K. Butlin; Corrado Caggese; Brian R. Calvi; A. Bernardo de Carvalho; Anat Caspi; Sergio Castrezana; Susan E. Celniker; Jean L. Chang; Charles Chapple; Sourav Chatterji; Asif Chinwalla; Alberto Civetta; Sandra W. Clifton; Josep M. Comeron; James C. Costello; Jerry A. Coyne; Jennifer Daub; Robert G. David; Arthur L. Delcher; Kim Delehaunty; Chuong B. Do; Heather Ebling; Kevin Edwards; Thomas Eickbush; Jay D. Evans; Alan Filipski; Sven Findeiss; Eva Freyhult; Lucinda Fulton; Robert Fulton; Ana C. L. Garcia; Anastasia Gardiner; David A. Garfield; Barry E. Garvin; Greg Gibson; Don Gilbert; Sante Gnerre; Jennifer Godfrey; Robert Good; Valer Gotea; Brenton Gravely; Anthony J. Greenberg; Sam Griffiths-Jones; Samuel Gross; Roderic Guigo; Erik A. Gustafson; Wilfried Haerty; Matthew W. Hahn; Daniel L. Halligan; Aaron L. Halpern; Gillian M. Halter; Mira V. Han; Andreas Heger; LaDeana Hillier; Angie S. Hinrichs; Ian Holmes; Roger A. Hoskins; Melissa J. Hubisz; Dan Hultmark; Melanie A. Huntley; David B. Jaffe; Santosh Jagadeeshan; William R. Jeck; Justin Johnson; Corbin D. Jones; William C. Jordan; Gary H. Karpen; Eiko Kataoka; Peter D. Keightley; Pouya Kheradpour; Ewen F. Kirkness; Leonardo B. Koerich; Karsten Kristiansen; Dave Kudrna; Rob J. Kulathinal; Sudhir Kumar; Roberta Kwok; Eric Lander; Charles H. Langley; Richard Lapoint; Brian P. Lazzaro; So-Jeong Lee; Lisa Levesque; Ruiqiang Li; Chiao-Feng Lin; Michael F. Lin; Kerstin Lindblad-Toh; Ana Llopart; Manyuan Long; Lloyd Low; Elena Lozovsky; Jian Lu; Meizhong Luo; Carlos A. Machado; Wojciech Makalowski; Mar Marzo; Muneo Matsuda; Luciano Matzkin; Bryant McAllister; Carolyn S. McBride; Brendan McKernan; Kevin McKernan; Maria Mendez-Lago; Patrick Minx; Michael U. Mollenhauer; Kristi Montooth; Stephen M. Mount; Xu Mu; Eugene Myers; Barbara Negre; Stuart Newfeld; Rasmus Nielsen; Mohamed A. F. Noor; Patrick O'Grady; Lior Pachter; Montserrat Papaceit; Matthew J. Parisi; Michael Parisi; Leopold Parts; Jakob S. Pedersen; Graziano Pesole; Adam M. Phillippy; Chris P. Ponting; Mihai Pop; Damiano Porcelli; Jeffrey R. Powell; Sonja Prohaska; Kim Pruitt; Marta Puig; Hadi Quesneville; Kristipati Ravi Ram; David Rand; Matthew D. Rasmussen; Laura K. Reed; Robert Reenan; Amy Reily; Karin A. Remington; Tania T. Rieger; Michael G. Ritchie; Charles Robin; Yu-Hui Rogers; Claudia Rohde; Julio Rozas; Marc J. Rubenfield; Alfredo Ruiz; Susan Russo; Steven L. Salzberg; Alejandro Sanchez-Gracia; David J. Saranga; Hajime Sato; Stephen W. Schaeffer; Michael C. Schatz; Todd Schlenke; Russell Schwartz; Carmen Segarra; Rama S. Singh; Laura Sirot; Marina Sirota; Nicholas B. Sisneros; Chris D. Smith; Temple F. Smith; John Spieth; Deborah E. Stage; Alexander Stark; Wolfgang Stephan; Robert L. Strausberg; Sebastian Strempel; David Sturgill; Granger Sutton; Granger G. Sutton; Wei Tao; Sarah Teichmann; Yoshiko N. Tobari; Yoshihiko Tomimura; Jason M. Tsolas; Vera L. S. Valente; Eli Venter; J. Craig Venter; Saverio Vicario; Filipe G. Vieira; Albert J. Vilella; Alfredo Villasante; Brian Walenz; Jun Wang; Marvin Wasserman; Thomas Watts; Derek Wilson; Richard K. Wilson; Rod A. Wing; Mariana F. Wolfner; Alex Wong; Gane Ka-Shu Wong; Chung-I Wu; Gabriel Wu; Daisuke Yamamoto; Hsiao-Pei Yang; Shiaw-Pyng Yang; James A. Yorke; Kiyohito Yoshida; Evgeny Zdobnov; Peili Zhang; Yu Zhang; Aleksey V. Zimin; Jennifer Baldwin; Amr Abdouelleil; Jamal Abdulkadir; Adal Abebe; Brikti Abera; Justin Abreu; St Christophe Acer; Lynne Aftuck; Allen Alexander; Peter An; Erica Anderson; Scott Anderson; Harindra Arachi; Marc Azer; Pasang Bachantsang; Andrew Barry; Tashi Bayul; Aaron Berlin; Daniel Bessette; Toby Bloom; Jason Blye; Leonid Boguslavskiy; Claude Bonnet; Boris Boukhgalter; Imane Bourzgui; Adam Brown; Patrick Cahill; Sheridon Channer; Yama Cheshatsang; Lisa Chuda; Mieke Citroen; Alville Collymore; Patrick Cooke; Maura Costello; Katie D'Aco; Riza Daza; Georgius De Haan; Stuart DeGray; Christina DeMaso; Norbu Dhargay; Kimberly Dooley; Erin Dooley; Missole Doricent; Passang Dorje; Kunsang Dorjee; Alan Dupes; Richard Elong; Jill Falk; Abderrahim Farina; Susan Faro; Diallo Ferguson; Sheila Fisher; Chelsea D. Foley; Alicia Franke; Dennis Friedrich; Loryn Gadbois; Gary Gearin; Christina R. Gearin; Georgia Giannoukos; Tina Goode; Joseph Graham; Edward Grandbois; Sharleen Grewal; Kunsang Gyaltsen; Nabil Hafez; Birhane Hagos; Jennifer Hall; Charlotte Henson; Andrew Hollinger; Tracey Honan; Monika D. Huard; Leanne Hughes; Brian Hurhula; M. Erii Husby; Asha Kamat; Ben Kanga; Seva Kashin; Dmitry Khazanovich; Peter Kisner; Krista Lance; Marcia Lara; William Lee; Niall Lennon; Frances Letendre; Rosie LeVine; Alex Lipovsky; Xiaohong Liu; Jinlei Liu; Shangtao Liu; Tashi Lokyitsang; Yeshi Lokyitsang; Rakela Lubonja; Annie Lui; Pen MacDonald; Vasilia Magnisalis; Kebede Maru; Charles Matthews; William McCusker; Susan McDonough; Teena Mehta; James Meldrim; Louis Meneus; Oana Mihai; Atanas Mihalev; Tanya Mihova; Rachel Mittelman; Valentine Mlenga; Anna Montmayeur; Leonidas Mulrain; Adam Navidi; Jerome Naylor; Tamrat Negash; Thu Nguyen; Nga Nguyen; Robert Nicol; Choe Norbu; Nyima Norbu; Nathaniel Novod; Barry O'Neill; Sahal Osman; Eva Markiewicz; Otero L. Oyono; Christopher Patti; Pema Phunkhang; Fritz Pierre; Margaret Priest; Sujaa Raghuraman; Filip Rege; Rebecca Reyes; Cecil Rise; Peter Rogov; Keenan Ross; Elizabeth Ryan; Sampath Settipalli; Terry Shea; Ngawang Sherpa; Lu Shi; Diana Shih; Todd Sparrow; Jessica Spaulding; John Stalker; Nicole Stange-Thomann; Sharon Stavropoulos; Catherine Stone; Christopher Strader; Senait Tesfaye; Talene Thomson; Yama Thoulutsang; Dawa Thoulutsang; Kerri Topham; Ira Topping; Tsamla Tsamla; Helen Vassiliev; Andy Vo; Tsering Wangchuk; Tsering Wangdi; Michael Weiand; Jane Wilkinson; Adam Wilson; Shailendra Yadav; Geneva Young; Qing Yu; Lisa Zembek; Danni Zhong; Andrew Zimmer; Zac Zwirko; David B. Jaffe; Pablo Alvarez; Will Brockman; Jonathan Butler; CheeWhye Chin; Sante Gnerre; Manfred Grabherr; Michael Kleber; Evan Mauceli; Iain MacCallum
  NATURE, 450, 7167, 203, 218, Nov. 2007
  English, Scientific journal
• Molecular cloning and characterization of Drosophila ornithine aminotransferase gene
  KM Yoshida; N Juni; SH Hori
  GENES & GENETIC SYSTEMS, 72, 1, 9, 17, Feb. 1997
  English, Scientific journal
• The Om(1E) mutation in Drosophila ananassae causes compound eye overgrowth due to tom retrotransposon-driven overexpression of a novel gene
  N Juni; T Awasaki; K Yoshida; SH Hori
  GENETICS, 143, 3, 1257, 1270, Jul. 1996
  English, Scientific journal
• An eye imaginal disc-specific transcriptional enhancer in the long terminal repeat of the tom retrotransposon is responsible for eye morphology mutations of Drosophila ananassae
  T Awasaki; N Juni; KM Yoshida
  MOLECULAR & GENERAL GENETICS, 251, 2, 161, 166, May 1996
  English, Scientific journal
• RETROTRANSPOSON-INDUCED ECTOPIC EXPRESSION OF THE OM(2D) GENE CAUSES THE EYE-SPECIFIC OM(2D) PHENOTYPE IN DROSOPHILA-ANANASSAE
  K YOSHIDA; N JUNI; T AWASAKI; Y TSURIYA; N SHAYA; SH HORI
  MOLECULAR & GENERAL GENETICS, 245, 5, 577, 587, Dec. 1994
  English, Scientific journal
• Retrotransposon-induced ectopic expression of cut causes the Om(1A) mutant in Drosophila ananassae.
  T Awasaki; N Juni; T Hamabata; K Yoshida; M Matsuda; Y N Tobari; S H Hori
  Genetics, 137, 1, 165, 74, May 1994, [Peer-reviewed], [International Magazine]
  English, Optic morphology (Om) mutations in Drosophila ananassae map to at least 22 loci scattered throughout the genome. They are semidominant, neomorphic, nonpleiotropic, and are associated with the insertion of a retrotransposon, tom. The Om(1A) gene, which is cytogenetically linked to the cut locus, was cloned using a DNA fragment of the cut locus of Drosophila melanogaster as a probe. Three of the eight alleles of Om(1A) examined have insertion of the tom element within a putative cut region. The gamma-ray-induced revertants of Om(1A) are accompanied with cut lethal mutations and rearrangements within the cut coding region. In the eye imaginal discs of the Om(1A) mutants, differentiation of photoreceptor clusters is suppressed, abnormal cell death occurs in the center and the cut protein is expressed ectopically. D. melanogaster flies transformed with a chimeric cut gene under the control of a heat-inducible promoter show excessive cell death in the region anterior to the morphogenetic furrow, suppressed differentiation to photoreceptor clusters and defect in the imaginal eye morphology when subjected to temperature elevation. These findings suggest that the tom element inserted within the Om(1A) region induces ectopic cut expression in the eye imaginal discs, thus resulting in the Om(1A) mutant phenotype.
• A Novel Fluorescent Method for in situ Hybridization.
  KAGIYAMA NAOTO; YOSHIDA KIYOHITO; HAMABATA TAKASHI; JUNI NAOTO; AWASAKI TAKESHI; FUJITA SATOSHI; MOMIYAMA MASAYOSHI; KONDOH YASUMITSU; YOSHIDA MICHIHIRO C.; HORI SAMUEL H.
  Proceedings of the Japanese Histochemical Association, 26, 5, 441, 445, JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY, 1993
  English

• 細菌における長鎖多価不飽和脂肪酸の膜リン脂質への転移メカニズムの研究
  橋本美佳子; 折笠善丈; 吉田磨仁, 日本生物工学会大会講演要旨集, 67th, 242, 242, 25 Sep. 2015
  日本生物工学会, Japanese
• ポリクローナル抗体を用いた細菌由来の多価不飽和脂肪酸合成酵素の検出
  牛膓健司; 橋本美佳子; 堀内綾乃; 横山愛; 吉田磨仁; 折笠善丈, 日本生物工学会大会講演要旨集, 67th, 96, 96, 25 Sep. 2015
  日本生物工学会, Japanese
• Brevibacillusを用いたドコサヘキサン酸合成遺伝子の発現
  橋本美佳子; 堀内綾乃; 吉田磨仁; 奥山英登志; 花方寛; 水上誠; 宮内明; 折笠善丈, 日本生物工学会大会講演要旨集, 66th, 29, 29, 05 Aug. 2014
  日本生物工学会, Japanese
• 高度好冷菌Colwellia psychrerythraea strain34Hの脂肪酸
  橋本美佳子; 折笠善丈; 吉田磨仁; 林秀則; 奥山英登志, 極域科学シンポジウム講演予稿集(CD-ROM), 5th, ROMBUNNO.OB,12GATSU3NICHI,10:50, 2014
  Japanese
• 細菌の長鎖多価不飽和脂肪酸合成酵素遺伝子
  吉田磨仁; 奥山英登志; 折笠善丈, オレオサイエンス, 13, 5, 211, 219, 01 May 2013
  In 1996, genes involved in the biosynthesis of a long-chain polyunsaturated fatty acid (LCPUFA), eicosapentaenoic acid (EPA), were first cloned from a bacterium isolated from the intestines of marine fish. This was approximately 10 years after the discovery of deep-sea psychrophilic bacteria capable of producing EPA and docosahexaenoic acid (DHA). Homologous genes now designated as pfa genes, including those responsible for the biosynthesis of DHA have been found in marine bacteria. These pfa genes generally consisted of five pfaA−pfaE genes encoding multifunctional proteins and were similar to those required for polyketide biosynthesis. The recombinant production of EPA and DHA mostly in Escherichia coli has been achieved. Although comparable pfa genes sequences can be retrieved from DNA databases, including those established using bacteria not only from cold temperature environment but also from subtropical and tropical regions, the distribution of typical pfa genes responsible for LCPUFA biosynthesis was restricted in some genera of marine γ-proteobacteria. Recent studies demonstrated that pfa genes, together with four oleA−oleD genes, were involved in the biosynthesis of a very long-chain polyunsaturated hydrocarbon, hentriacontanonaene (C31:9). Interestingly a set of pfa and ole genes was presumed to be distributed in all LCPUFA-producing bacteria and even in some strictly anaerobic bacteria that contain C31:9. This review describes the structure of bacterial pfa genes responsible for the biosynthesis of EPA and DHA, and their distribution in marine bacterial lineages and possible usage for practical production of LCPUFAs., Japan Oil Chemists' Society, Japanese
• Identification of the Om(1J)Su gene, a suppressor of tom-induced Om mutations in Drosophila ananassae
  Kiyohito Yoshida; Monica L. Acosta, GENES & GENETIC SYSTEMS, 85, 6, 432, 432, Dec. 2010
  English, Summary international conference
• Identification of the Om(1J)Su gene, a suppressor of tom-induced Om mutations in Drosophila ananassae
  Kiyohito Yoshida; Monica L. Acosta, GENES & GENETIC SYSTEMS, 84, 6, 440, 440, Dec. 2009
  English, Summary international conference
• Om(1E) mutation of ananas Drosophila. Hyperplasy of compound eye induced by insertion of retrotransposon tom.
  従二直人; 浜端崇; 吉田磨仁; 粟崎健; 堀浩, 日本遺伝学会大会プログラム・予稿集, 66th, 1994
• Relation between compound eye form mutation Om and transposable factor tom of ananas Drosophila.
  粟崎健; 従二直人; 吉田磨仁; 堀浩, 日本遺伝学会大会プログラム・予稿集, 66th, 1994
• アナナスショウジョウバエの複眼形態突然変異Om(2C)遺伝子のクローニング
  中村英明; 粟崎健; 従二直人; 吉田磨仁; 堀浩, 日本遺伝学会大会プログラム・予稿集, 65th, 1993
• 転移因子tomにより引き起こされるアナナスショウジョウバエの複眼形態突然変異,Om(1A)突然変異の解析
  粟崎健; 従二直人; 松田宗男; 吉田磨仁; 堀浩; 戸張よし子, 日本遺伝学会大会プログラム・予稿集, 65th, 1993
• DNASE I-HYPERSENSITIVE SITES AROUND THE G6PD CODING REGION IN DROSOPHILA-MELANOGASTER
  K YOSHIDA; SH HORI, JAPANESE JOURNAL OF GENETICS, 62, 6, 567, 568, Dec. 1987
  Japanese, Summary international conference

• 環境科学研究基礎論, 2024年, 修士課程, 環境科学院
• 生態遺伝学特論Ⅱ, 2024年, 修士課程, 環境科学院
• 細胞生物学概論, 2024年, 学士課程, 理学部
• ISP生物科学実習Ⅰ・a, 2024年, 学士課程, 理学部
• ISP生物科学実習Ⅰ・b, 2024年, 学士課程, 理学部
• 遺伝学実習, 2024年, 学士課程, 理学部
• 遺伝学実習, 2024年, 学士課程, 総合教育部
• 環境生物学Ⅱ, 2024年, 学士課程, 理学部
• 英語演習, 2024年, 学士課程, 全学教育

• The Society for Biotechnology, Japan

• ナミテントウの翅鞘斑紋パターン形成に関与する遺伝子の分離固定
  科学研究費助成事業
  1998 - 1999
  吉田 磨仁
  ナミテントウ(Harmonia axyridis)は,生態学,応用昆虫学の研究材料として古くから注目されてきた。本種の翅鞘斑紋は遺伝的多型の例として知られ、赤地型、二紋型、四紋型、多紋型などが知られている。遺伝学的研究によれば、斑紋を支配する遺伝子は30以上知られており、これらの遺伝子はすべて同じかもしくはきわめて近接した遺伝子座の複対立遺伝子であると考えられている。本研究では斑紋形式のメカニズムの解明の第一歩として、斑紋形成に関わる遺伝子の探求を以下の2つの方法で試みた。
  鱗翅目昆虫では、Distal-less(Dll)遺伝子が斑紋を形成する翅始原組織の細胞群で発現していることが明らかになっている。そこでDll遺伝子が、ナミテントウにおいても斑紋状に発現している可能性について調査した。組織学的観察の結果、翅原基の形成は4齢虫期に開始することが明らかになったので、4齢幼虫、前蛹、蛹期の翅原基でのDll遺伝子の発現をチョウのDll蛋白に対する抗体を用いて調べた。その結果、4齢虫期の翅原基においてはこの遺伝子は翅の縁の沿って発現しており、斑紋状には発現していなかった。従ってナミテントウではDll遺伝子は、斑紋形成には直接は関与していないと考えられた。
  次に、斑紋形成に関与する遺伝子を単離することを目的に、翅原基に特異的なcDNAライブラリーを作成することにした。まず、4齢、前蛹、蛹の各段階の翅原基からcDNAライブラリーを作成した。次に、前蛹のcDNAから4齢のcDNAと共通に存在するものをハイブリダイゼーションを利用して除去、前蛹期に発現が始まる遺伝子のcDNAとした。また、前蛹のcDNAと蛹のcDNAとに共通するものを除去し、蛹期に発現が減少する遺伝子のcDNAとした。以上の結果、10個の遺伝子が分離された。これらのcDNAの塩基配列を決定し、ホモロジー検索をした結果、これまで知られている遺伝子と高い相同性は示されなかった。今後、得られたcDNAを用いて本種翅始原組織における発現場所を調べ、これらの遺伝子が斑紋形成に関係しているかどうかを明らかにしたい。
  日本学術振興会, 奨励研究(A), 北海道大学, 10740349
• ショウジョウバエの動く遺伝子による突然変異誘発機構の研究
  1995
  Competitive research funding
• Genetic analysis of the transposon in Drosophila
  1995
  Competitive research funding
• Transcriptional activation of the Drosophila melanogaster glucose-6- phosphate dehydrogenase gene by insertion of defective P elements
  Grants-in-Aid for Scientific Research
  1992 - 1993
  HORI Hiroshi; YOSHIDA Kiyohito
  Tandem insertions of defective P elements (1.15 kb KP and 0.6 kb core P) accelerate the transcription rate of the glucose-6-phosphate dehydrogenase (G6PD) gene in Drosophila melanogaster. In this resarch, we have analyzed the activation mechanism of G6PD promoter by in vitro transcription and gel retardation assays. Results showed that on cis-acting region in the core P and two such regions in the KP are associated with the activation of G6PD promoter, and that putative transcriptional regulatory protein(s) which specifically bind to each of the cis-acting regions are present in nuclear extracts of Canton S embryos. On the other hand, the P elements do not activate the normal actin 5C promoter, but activate the promoter when the 20 bp sequence around the G6PD transcription start site is placed in front of the promoter. It seems that the GC-rich region in the 20 bp sequence is required for the activation.
  Japan Society for the Promotion of Science, Grant-in-Aid for General Scientific Research (C), HOKKAIDO UNIVERSITY, 04640590
• A novel fluorescent method for detection of subpicogram quantities of nucleic acids
  Grants-in-Aid for Scientific Research
  1990 - 1991
  HORI Hiroshi; YOSHIDA Kiyohito; YOSHIDA Michihiro; SHIRAHAMA Haruhisa
  In recombinant DNA techniques, it is required to detect a very small amount of nucleic acids. In standard protocols this is accomplished by the use of radio-labeled nucleic acids as probes. However, the inherent drawbacks of radiolabeled probes, such as chemical lability due to radiolytic decomposition, personnel safety and disposal problems, short half-life and duration of autoradiographic exposure limit the usefulness of radio-labeled probes in conventional laboratories. It is therefore desirable to have sensitive, non-radioisotope methods for detecting nucleic acids. Recontly, efforts have been made in a number of laboratories to innovate a new non-radioisotopic methods and a variety of methods have been proposed. Among these, the Boehringer's Digoxigenin-alkaline phosphosphatase method utilizing either BCIP-Nitro BT or a chemiluminescent substrate, AMPPD as a substrate appears to be most reliable and sensitive method. However, the method is still lower in sensitivity than the RI method. To improve the sensitivity of thenon-RI method, we have attempted in this profect to synthesize fluorogenic substrates for alkalien phosphatase which upon hydrolysis yield insoluble, highly fluorescent products. As a result, HNPP (3-hydroxy-N-2-biphenylyl-2-naphthalenecarboxamide phosphate) was found to be the most useful substrate among a total of 86 fluorochromes synthesized. With this substrate, it is possible to detact as small as 10fg of DNA by spot tests, and 70fg of DNA by southern blots. The method ustilizing HNPP is superior to any available techniques with respect to resolution, speed, ease of reaction monitoring, data storage, reprobing and stability of reagents.
  Japan Society for the Promotion of Science, Grant-in-Aid for Developmental Scientific Research (B), Hokkaido University, 02559002