Researcher basic information

• 博士（農学）, 北海道大学

• https://researchmap.jp/read0055143?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200901056451421836

• 200901056451421836

• Intestinal colonization
• Bile-acid transforming bacteria
• Transposon
• Bifidobacterium
• 変異導入
• デオキシコール酸
• 二次胆汁酸
• コール酸
• カルジオリピン
• リン脂質
• 腸内生存
• 胆汁酸
• 乳酸菌
• 微生物遺伝・育種
• Molecular Microbiology
• Microbial genetics and physiology

• Life Science, Applied microbiology

• Bachelor's degree program, School of Agriculture
• Master's degree program, Graduate School of Agriculture
• Doctoral (PhD) degree program, Graduate School of Agriculture

Research activity information

• Oct. 2015, Society for Biotechnology, Japan, Encouragement Award of the Society for Biotechnology, Japan (Saito Award)
  Development of practical gene-mutagenesis systems in bifidobacteria
  FUKIYA Satoru

• Identification and characterization of Dorea ammoniilytica as a novel deoxycholic acid-producing bacterial species in the human gut microbiota
  Ni Wayan Eka Putri Gayatri Kastawa; Yasuhiro Gotoh; Isaiah Song; Tomoya Maeda; Satoru Fukiya
  Bioscience, Biotechnology, and Biochemistry, 90, 3, 402, 413, Oxford University Press (OUP), 18 Dec. 2025, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal, Abstract
  
  Deoxycholic acid (DCA), a representative secondary bile acid, is produced by specific gut bacteria through bile acid 7α-dehydroxylation of cholic acid, catalyzed by enzymes encoded in the bai gene operon. Exploration of diversity and functional characteristics of DCA-producing bacteria is crucial for understanding the “in vivo” mechanisms of DCA production in the human intestine. Here, we have identified and characterized two strains derived from human feces as a novel DCA-producing species, Dorea ammoniilytica. These strains harbored segmented bai gene operons in their complete genome sequences and showed high DCA production activity from cholic acid in the culture experiments. Biochemical, phylogenetic, and average nucleotide identity analyses categorized them as D. ammoniilytica, which belongs to a distinct lineage from other known DCA producers and Dorea species. These findings expand the diversity of secondary bile acid-producing bacteria in the human gut microbiota and provide clues for clarifying the in vivo DCA production mechanisms.
• A unique tetrasaccharide-containing anchor glycolipid of lipoteichoic acid is commonly found in Lactobacillus gasseri and Lactobacillus paragasseri.
  Tsukasa Shiraishi; Ryosuke Kutomi; Yamaha Sato; Akihito Endo; Satoru Fukiya; Satoshi Takahashi; Atsushi Yokota; Shin-Ichi Yokota
  Bioscience, biotechnology, and biochemistry, 89, 9, 1382, 1390, 23 Aug. 2025, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Lipoteichoic acid (LTA) is a cell surface molecule with physiologically important roles in Gram-positive bacteria. Previously, we identified a novel tetrasaccharide-containing anchor glycolipid in the Lactobacillus gasseri JCM 1131T LTA. However, it is unclear whether this tetrasaccharide-containing anchor glycolipid structure is characteristic of JCM 1131T alone or is commonly found in other L. gasseri-related isolates. Lactobacillus paragasseri has been proposed as a new species, separated from the L. gasseri. In the present study, we characterized the chemical structures of LTA from 2 strains of L. gasseri and 4 strains of L. paragasseri. All 6 strains shared a polymer comprising repeating units of glycerophosphate with partial D-alanine substitutions, as well as L. gasseri JCM 1131T. Anchor glycolipid structure suggested that all strains shared a tetrasaccharide-containing glyceroglycolipid with 2 and 3 acyl groups. Focusing on LTA from L. gasseri and L. paragasseri species, a tetrasaccharide-containing anchor glycolipid is a specific feature.
• Bile acid is a responsible host factor for high-fat diet-induced gut microbiota alterations in rats: proof of the "bile acid hypothesis".
  Masamichi Watanabe; Yuki Fujita; Masahito Hagio; Satoshi Ishizuka; Yoshitoshi Ogura; Tetsuya Hayashi; Satoru Fukiya; Atsushi Yokota
  Bioscience of microbiota, food and health, 44, 2, 110, 121, 2025, [Peer-reviewed], [Corresponding author], [Domestic magazines]
  English, Scientific journal, High-fat diet (HFD)-induced alterations in gut microbiota may be associated with host pathophysiology, prompting increased interest in elucidating their causal relationships. However, the mechanisms by which HFDs induce these alterations require further clarification. Our previous study using cholic acid (CA)-fed rats suggested that bile acid drives the HFD-induced microbiota alterations as a host factor, a concept termed the "bile acid hypothesis". We analyzed the alterations in the cecal microbiota and bile acid composition in HFD-fed rats and compared the results with those of rats on a CA-supplemented diet. In both cases, the concentrations of total bile acids, including highly bactericidal deoxycholic acid (DCA), increased, concomitant with the increases in the Firmicutes (Bacillota)/Bacteroidetes (Bacteroidota) ratio. Operational taxonomic units (OTUs), accounting for 63.39% of the cecal microbiota of control rats, showed a significant correlation with the total bile acid concentration in HFD-fed rats. A DCA sensitivity test conducted in Firmicutes isolates, corresponding to the predominant OTUs from the HFD-fed rats, exhibited significantly higher DCA resistance compared with Bacteroidetes. The top 12 most abundant OTUs of Firmicutes and Bacteroidetes showing positive or negative correlations with the total bile acid concentration were selected from the HFD-fed rats, and their dynamics were compared with those in the CA-fed rats. Of the 24 OTUs, 18, which constituted 48.28% of the cecal population in the control rats, were altered in the same direction (increase or decrease) in the HFD- and CA-supplemented diet groups. Therefore, approximately half of the cecal populations in the control rats were affected by bile acids, substantiating the bile acid hypothesis microbiologically and quantitatively.
• Improved fermentative gamma-aminobutyric acid production from glucose by the inactivation of respiratory chain components NDH-I and Cytbo₃ in Escherichia coli.
  Hiroki Wakahara; Takuya Mizokoshi; Kotaro Yamagami; Satoru Fukiya; Atsushi Yokota; Tomoya Maeda
  Journal of bioscience and bioengineering, 138, 6, 501, 506, Dec. 2024, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Gamma-aminobutyric acid (GABA), which is synthesized from l-glutamic acid via glutamate decarboxylase (Gad), is used as food, supplements, and biodegradable plastics. Our previous study demonstrated an Escherichia coli mutant (ΔΔ) strain, lacking type I NADH dehydrogenase (NDH-I) and cytochrome bo3 oxidase (Cytbo3), produced 7 g/L glutamic acid on MS1 glucose-minimal medium. In this study, the ΔΔ strain was used for improving GABA production. A plasmid (pMBL19-gadB') expressing a mutated E. coli GadB (Glu89Gln/Δ452-466), retaining activity at neutral pH, was introduced into the ΔΔ strain and its parent strain (W1485). The ΔΔ strain carrying pMBL19-gadB' exhibited a twofold increase in GABA production compared to the W1485 strain carrying pMBL19-gadB'. Deleting the C-terminal (Δ471-511) of GadC antiporter in the ΔΔ strain further improved GABA yield to 1.5 g/L when cultured in MS1 glucose-minimal medium. On the other hand, a large amount of glutamic acid produced by the ΔΔ strain was not fully converted to GABA, likely due to the inhibition of GadB activity by the accumulation of acetic acid. Although there is room for improvement, these results indicate the efficacy of the ΔNDH-IΔCytbo3 double mutation in augmenting GABA production.
• Contributions of the anaplerotic reaction enzymes pyruvate carboxylase and phosphoenolpyruvate carboxylase to l-lysine production in Corynebacterium glutamicum.
  Akinobu Shinmori; Zhen Guo; Tomoya Maeda; Satoru Fukiya; Masaru Wada; Atsushi Yokota
  Journal of bioscience and bioengineering, 27 Jun. 2024, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Anaplerotic reactions catalyzed by pyruvate carboxylase (PC) and phosphoenolpyruvate carboxylase (PEPC) have important roles in the production of l-lysine to replenish oxaloacetic acid (OAA) in Corynebacterium glutamicum. However, the relative contributions of these enzymes to l-lysine production in C. glutamicum are not fully understood. In this study, using a parent strain (P) carrying a feedback inhibition-resistant aspartokinase with the T311I mutation, we constructed a PC gene-deleted mutant strain (PΔPC) and a PEPC gene-deleted mutant strain (PΔPEPC). Although the growth of both mutant strains was comparable to the growth of strain P, the maximum l-lysine production in strains PΔPC and PΔPEPC decreased by 14% and 49%, respectively, indicating that PEPC strongly contributed to OAA supply. l-Lysine production in strain PΔPC slightly decreased during the logarithmic phase, while production during the early stationary phase was comparable to production in strain P. By contrast, strain PΔPEPC produced l-lysine in an amount comparable to the production of strain P during the logarithmic phase; l-lysine production after the early stationary phase was completely stopped in strain PΔPEPC. These results indicate that OAA is supplied by both PC and PEPC during the logarithmic phase, while only PEPC can continuously supply OAA after the logarithmic phase.
• Synthesis and quantification of short-chain fatty acid esters of hydroxy fatty acids in rat intestinal contents and fecal samples by LC-MS/MS
  Siddabasave Gowda B. Gowda; Fengjue Hou; Divyavani Gowda; Hitoshi Chiba; Kentaro Kawakami; Satoru Fukiya; Atsushi Yokota; Shu-Ping Hui
  Analytica Chimica Acta, 1288, 342145, 342145, Elsevier BV, Feb. 2024
  Scientific journal
• Development of an improved colonization system for human-derived Bifidobacterium longum subsp. longum in conventional mice through the feeding of raffinose or 1-kestose.
  Mina Shimada; Youhei Kawase; Kei Sonoyama; Yoshitoshi Ogura; Tetsuya Hayashi; Atsushi Yokota; Satoru Fukiya
  Bioscience of microbiota, food and health, 43, 2, 110, 119, 2024, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
  English, Scientific journal, How bifidobacteria colonize and survive in the intestine is not fully understood. The administration of bifidobacteria to conventional mice can be used to evaluate their ability to colonize the intestine in the presence of endogenous gut microbiota. However, human-derived bifidobacteria do not readily colonize the intestines of conventional mice, and although colonization by Bifidobacterium breve UCC2003 has been achieved, the viability of such populations requires improvement. Therefore, we aimed to establish a colonization system with human-derived bifidobacteria of high viability in conventional mice using Bifidobacterium longum subsp. longum 105-A. Lactose, raffinose, and 1-kestose were identified as the preferred carbohydrate sources for the growth of this strain in culture. The administration of B. longum 105-A to conventional BALB/c mice fed these carbohydrates showed that diets containing 6% (w/w) raffinose or 1-kestose facilitated colonization with >108 colony-forming units/g feces for 2 weeks. The population of this strain was more stable in the raffinose-fed group than in the 1-kestose-fed group. The ingestion of these prebiotics had a greater impact on the composition of the microbiota than the administration of B. longum 105-A. The ingestion of these prebiotics also increased the fecal concentrations of organic acids, which was indicative of greater intestinal fermentation. Collectively, we established a colonization system for B. longum 105-A with high viability in conventional mice by feeding the mice raffinose or 1-kestose. This system should be useful for elucidation of the mechanisms of colonization and survival of bifidobacteria in the intestines in the presence of the endogenous gut microbiota.
• Claveliimonas bilis gen. nov., sp. nov., deoxycholic acid-producing bacteria isolated from human faeces, and reclassification of Sellimonas monacensis Zenner et al. 2021 as Claveliimonas monacensis comb. nov.
  Atsushi Hisatomi; Ni Wayan Eka Putri Gayatri Kastawa; Isaiah Song; Moriya Ohkuma; Satoru Fukiya; Mitsuo Sakamoto
  International Journal of Systematic and Evolutionary Microbiology, 73, 9, Microbiology Society, 21 Sep. 2023, [Peer-reviewed]
  Scientific journal, Obligately anaerobic, Gram-stain-positive, bacilli, strains 12BBH14^T, 9CFEGH4 and 10CPCBH12, were isolated from faecal samples of healthy Japanese people. Strain 12BBH14^T showed the highest 16S rRNA gene sequence similarity to Sellimonas monacensis Cla-CZ-80^T (97.5 %) and ‘Lachnoclostridium phocaeense’ Marseille-P3177^T (97.2 %). Strain 12BBH14^T was also closely related to Eubacterium sp. c-25 with 99.7 % 16S rRNA gene sequence similarity. The 16S rRNA gene sequence analysis showed that strains 12BBH14^T, 9CFEGH4 and 10CPCBH12 formed a monophyletic cluster with Eubacterium sp. c-25. Near this monophyletic cluster, S. monacensis Cla-CZ-80^T and ‘L. phocaeense’ Marseille-P3177^T formed a cluster and did not form a cluster with other Sellimonas species. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 12BBH14^T, 9CFEGH4, 10CPCBH12 and Eubacterium sp. c-25 were higher than the cut-off values of species demarcation (>88 % dDDH and >98 % ANI), indicating that these four strains are the same species. On the other hand, the dDDH and ANI values of these strains were lower than the cut-off values of species demarcation against other strains (<29 % dDDH and <76 % ANI). Moreover, the average amino acid identity values among these strains were higher than the genus boundary. These results indicate that the isolates should be considered to belong to a new genus of the family Lachnospiraceae. Based on the collected data, strains 12BBH14^T, 9CFEGH4 and 10CPCBH12 represent a novel species of a novel genus, for which the name Claveliimonas bilis gen. nov., sp. nov. is proposed. The type strain of C. bilis is 12BBH14^T (=JCM 35899^T=DSM 115701^T). Eubacterium sp. c-25 belongs to C. bilis. In addition, S. monacensis is transferred to the genus Claveliimonas as Claveliimonas monacensis comb. nov.
• Diversification of a Fucosyllactose Transporter within the Genus Bifidobacterium.
  Miriam N Ojima; Yuya Asao; Aruto Nakajima; Toshihiko Katoh; Motomitsu Kitaoka; Aina Gotoh; Junko Hirose; Tadasu Urashima; Satoru Fukiya; Atsushi Yokota; Maher Abou Hachem; Mikiyasu Sakanaka; Takane Katayama
  Applied and environmental microbiology, 88, 2, e0143721, 25 Jan. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Human milk oligosaccharides (HMOs), which are natural bifidogenic prebiotics, were recently commercialized to fortify formula milk. However, HMO assimilation phenotypes of bifidobacteria vary by species and strain, which has not been fully linked to strain genotype. We have recently shown that specialized uptake systems, particularly for the internalization of major HMOs (fucosyllactose [FL]), are associated with the formation of a Bifidobacterium-rich gut microbial community. Phylogenetic analysis revealed that FL transporters have diversified into two clades harboring four clusters within the Bifidobacterium genus, but the underpinning functional diversity associated with this divergence remains underexplored. In this study, we examined the HMO consumption phenotypes of two bifidobacterial species, Bifidobacterium catenulatum subsp. kashiwanohense and Bifidobacterium pseudocatenulatum, both of which possess FL-binding proteins that belong to phylogenetic clusters with unknown specificities. Growth assays, heterologous gene expression experiments, and HMO consumption analyses showed that the FL transporter type from B. catenulatum subsp. kashiwanohense JCM 15439T conferred a novel HMO uptake pattern that includes complex fucosylated HMOs (lacto-N-fucopentaose II and lacto-N-difucohexaose I/II). Further genomic landscape analyses of FL transporter-positive bifidobacterial strains revealed that the H-antigen- or Lewis antigen-specific fucosidase gene(s) and FL transporter specificities were largely aligned. These results suggest that bifidobacteria have acquired FL transporters along with the corresponding gene sets necessary to utilize the imported HMOs. Our results provide insight into the species- and strain-dependent adaptation strategies of bifidobacteria in HMO-rich environments. IMPORTANCE The gut of breastfed infants is generally dominated by health-promoting bifidobacteria. Human milk oligosaccharides (HMOs) from breast milk selectively promote the growth of specific taxa such as bifidobacteria, thus forming an HMO-mediated host-microbe symbiosis. While the coevolution of humans and bifidobacteria has been proposed, the underpinning adaptive strategies employed by bifidobacteria require further research. Here, we analyzed the divergence of the critical fucosyllactose (FL) HMO transporter within Bifidobacterium. We have shown that the diversification of the solute-binding proteins of the FL transporter led to uptake specificities of fucosylated sugars ranging from simple trisaccharides to complex hexasaccharides. This transporter and the congruent acquisition of the necessary intracellular enzymes allow bifidobacteria to consume different types of HMOs in a predictable and strain-dependent manner. These findings explain the adaptation and proliferation of bifidobacteria in the competitive and HMO-rich infant gut environment and enable accurate specificity annotation of transporters from metagenomic data.
• Comparative Genomic and Physiological Analysis against Clostridium scindens Reveals Eubacterium sp. c-25 as an Atypical Deoxycholic Acid Producer of the Human Gut Microbiota.
  Isaiah Song; Yasuhiro Gotoh; Yoshitoshi Ogura; Tetsuya Hayashi; Satoru Fukiya; Atsushi Yokota
  Microorganisms, 9, 11, 29 Oct. 2021, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, The human gut houses bile acid 7α-dehydroxylating bacteria that produce secondary bile acids such as deoxycholic acid (DCA) from host-derived bile acids through enzymes encoded by the bai operon. While recent metagenomic studies suggest that these bacteria are highly diverse and abundant, very few DCA producers have been identified. Here, we investigated the physiology and determined the complete genome sequence of Eubacterium sp. c-25, a DCA producer that was isolated from human feces in the 1980s. Culture experiments showed a preference for neutral to slightly alkaline pH in both growth and DCA production. Genomic analyses revealed that c-25 is phylogenetically distinct from known DCA producers and possesses a multi-cluster arrangement of predicted bile-acid inducible (bai) genes that is considerably different from the typical bai operon structure. This arrangement is also found in other intestinal bacterial species, possibly indicative of unconfirmed 7α-dehydroxylation capabilities. Functionality of the predicted bai genes was supported by the induced expression of baiB, baiCD, and baiH in the presence of cholic acid substrate. Taken together, Eubacterium sp. c-25 is an atypical DCA producer with a novel bai gene cluster structure that may represent an unexplored genotype of DCA producers in the human gut.
• Next-generation prebiotic promotes selective growth of bifidobacteria, suppressing Clostridioides difficile
  Rika Hirano; Mikiyasu Sakanaka; Kazuto Yoshimi; Naohisa Sugimoto; Syogo Eguchi; Yuko Yamauchi; Misaki Nara; Shingo Maeda; Yuta Ami; Aina Gotoh; Takane Katayama; Noriho Iida; Tamotsu Kato; Hiroshi Ohno; Satoru Fukiya; Atsushi Yokota; Mamoru Nishimoto; Motomitsu Kitaoka; Hiroyuki Nakai; Shin Kurihara
  Gut Microbes, 13, 1, e1973835, Informa UK Limited, 23 Sep. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Dietary raffinose ameliorates hepatic lipid accumulation induced by cholic acid via modulation of enterohepatic bile acid circulation in rats.
  Kenta Maegawa; Haruka Koyama; Satoru Fukiya; Atsushi Yokota; Koichiro Ueda; Satoshi Ishizuka
  The British journal of nutrition, 127, 11, 1, 10, 14 Jul. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Enterohepatic circulation of 12α-hydroxylated (12αOH) bile acid (BA) is enhanced depending on the energy intake in high-fat diet-fed rats. Such BA metabolism can be reproduced using a diet supplemented with cholic acid (CA), which also induces simple steatosis, without inflammation and fibrosis, accompanied by some other symptoms that are frequently observed in the condition of non-alcoholic fatty liver in rats. We investigated whether supplementation of the diet with raffinose (Raf) improves hepatic lipid accumulation induced by the CA-fed condition in rats. After acclimation to the AIN-93-based control diet, male Wistar rats were fed diets supplemented with a combination of Raf (30 g/kg diet) and/or CA (0·5 g/kg diet) for 4 weeks. Dietary Raf normalised hepatic TAG levels (two-way ANOVA P < 0·001 for CA, P = 0·02 for Raf and P = 0·004 for interaction) in the CA-supplemented diet-fed rats. Dietary Raf supplementation reduced hepatic 12αOH BA concentration (two-way ANOVA P < 0·001 for CA, P = 0·003 for Raf and P = 0·03 for interaction). The concentration of 12αOH BA was reduced in the aortic and portal plasma. Raf supplementation increased acetic acid concentration in the caecal contents (two-way ANOVA P = 0·001 as a main effect). Multiple regression analysis revealed that concentrations of aortic 12αOH BA and caecal acetic acid could serve as predictors of hepatic TAG concentration (R2 = 0·55, P < 0·001). However, Raf did not decrease the secondary 12αOH BA concentration in the caecal contents as well as the transaminase activity in the CA diet-fed rats. These results imply that dietary Raf normalises hepatic lipid accumulation via suppression of enterohepatic 12αOH BA circulation.
• Primary 12α-Hydroxylated Bile Acids Lower Hepatic Iron Concentration in Rats.
  Shota Hori; Minako Satake; Ohji Kohmoto; Ryo Takagi; Kazufumi Okada; Satoru Fukiya; Atsushi Yokota; Satoshi Ishizuka
  The Journal of nutrition, 151, 3, 523, 530, 11 Mar. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: Primary 12α-hydroxylated bile acids (12αOH BAs) enhance intestinal iron uptake due to their ability ex vivo to chelate iron. However, no information is available on their role in vivo, especially in the liver. OBJECTIVES: To investigate the effects and mechanisms of primary 12αOH BAs on hepatic iron concentration in vivo. METHODS: Male Wistar King A Hokkaido male rats (WKAH/HkmSlc) rats aged 4-5 weeks were fed a control diet or a diet with cholic acid (CA; 0.5 g/kg diet), the primary 12αOH BA, for 2 weeks (Study 1) or 13 weeks (Study 2). In Study 3, rats fed the same diets were given drinking water either alone or containing vancomycin (200 mg/L) for 6 weeks. The variables measured included food intake (Studies 1-3), bile acid profiles (Studies 1 and 3), hepatic iron concentration (Studies 1-3), fecal iron excretion (Studies 1 and 2), iron-related liver gene expression (Studies 2 and 3), and plasma iron-related factors (Studies 2 and 3). RESULTS: In Study 1, CA feed reduced the hepatic iron concentration (-16%; P = 0.005) without changing food intake or fecal iron excretion. In Study 2, we found a significant increase in the aortic plasma concentration of lipocalin 2 (LCN2; +65%; P < 0.001), an iron-trafficking protein. In Study 3, we observed no effect of vancomycin treatment on the CA-induced reduction of hepatic iron concentration (-32%; P < 0.001), accompanied by increased plasma LCN2 concentration (+72%; P = 0.003), in the CA-fed rats despite a drastic reduction in the secondary 12αOH BA concentration (-94%; P < 0.001) in the aortic plasma. CONCLUSIONS: Primary 12αOH BAs reduced the hepatic iron concentration in rats. LCN2 may be responsible for the hepatic iron-lowering effect of primary 12αOH BAs by transporting iron out of the liver.
• Methionine utilization by bifidobacteria: possible existence of a reverse transsulfuration pathway.
  Masaru Wada; Satoru Fukiya; Azusa Suzuki; Nanae Matsumoto; Miki Matsuo; Atsushi Yokota
  Bioscience of microbiota, food and health, 40, 1, 80, 83, 2021, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Although bifidobacteria are already widely used as beneficial microbes with health-promoting effects, their amino acid utilization and metabolism are not yet fully understood. Knowledge about the metabolism of sulfur-containing amino acids in bifidobacteria is especially limited. In this study, we tested the methionine utilization ability of several bifidobacterial strains when it was the sole available sulfur source. Although bifidobacteria have long been predominantly considered to be cysteine auxotrophs, we showed that this is not necessarily the case.
• 12α-Hydroxylated bile acid induces hepatic steatosis with dysbiosis in rats.
  Ja-Young Lee; Hidehisa Shimizu; Masahito Hagio; Satoru Fukiya; Masamichi Watanabe; Yasutake Tanaka; Ga-Hyun Joe; Hitoshi Iwaya; Reika Yoshitsugu; Keidai Kikuchi; Misaki Tsuji; Nanako Baba; Takuma Nose; Koji Tada; Taketo Hanai; Shota Hori; Akari Takeuchi; Yumiko Furukawa; Bungo Shirouchi; Masao Sato; Tadasuke Ooka; Yoshitoshi Ogura; Tetsuya Hayashi; Atsushi Yokota; Satoshi Ishizuka
  Biochimica et biophysica acta. Molecular and cell biology of lipids, 1865, 12, 158811, 158811, Dec. 2020, [Peer-reviewed], [Lead author], [International Magazine]
  English, Scientific journal, There is an increasing need to explore the mechanism of the progression of non-alcoholic fatty liver disease. Steroid metabolism is closely linked to hepatic steatosis and steroids are excreted as bile acids (BAs). Here, we demonstrated that feeding WKAH/HkmSlc inbred rats a diet supplemented with cholic acid (CA) at 0.5 g/kg for 13 weeks induced simple steatosis without obesity. Liver triglyceride and cholesterol levels were increased accompanied by mild elevation of aminotransferase activities. There were no signs of inflammation, insulin resistance, oxidative stress, or fibrosis. CA supplementation increased levels of CA and taurocholic acid (TCA) in enterohepatic circulation and deoxycholic acid (DCA) levels in cecum with an increased ratio of 12α-hydroxylated BAs to non-12α-hydroxylated BAs. Analyses of hepatic gene expression revealed no apparent feedback control of BA and cholesterol biosynthesis. CA feeding induced dysbiosis in cecal microbiota with enrichment of DCA producers, which underlines the increased cecal DCA levels. The mechanism of steatosis was increased expression of Srebp1 (positive regulator of liver lipogenesis) through activation of the liver X receptor by increased oxysterols in the CA-fed rats, especially 4β-hydroxycholesterol (4βOH) formed by upregulated expression of hepatic Cyp3a2, responsible for 4βOH formation. Multiple regression analyses identified portal TCA and cecal DCA as positive predictors for liver 4βOH levels. The possible mechanisms linking these predictors and upregulated expression of Cyp3a2 are discussed. Overall, our observations highlight the role of 12α-hydroxylated BAs in triggering liver lipogenesis and allow us to explore the mechanisms of hepatic steatosis onset, focusing on cholesterol and BA metabolism.
• Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents.
  Siddabasave Gowda B Gowda; Divyavani Gowda; Chongsheng Liang; Yonghan Li; Kentaro Kawakami; Satoru Fukiya; Atsushi Yokota; Hitoshi Chiba; Shu-Ping Hui
  Metabolites, 10, 10, 08 Oct. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are novel endogenous lipids with important physiological functions in mammals. We previously identified a new type of FAHFAs, named short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs), with acetyl or propyl esters of hydroxy fatty acids of carbon chains, C ≥ 20. However, sensitive determination of SFAHFAs is still a challenge, due to their high structural similarity and low abundance in biological samples. This study employs one-step chemical derivatization following total lipid extraction using 2-dimethylaminoethylamine (DMED) for enhanced detection of SFAHFAs. The labeled extracts were subjected to ultrahigh performance liquid chromatography coupled to linear ion trap quadrupole-Orbitrap mass spectrometry (UHPLC/LTQ-Orbitrap MS). Our results demonstrated that the detection sensitivities of SFAHFAs increased after DMED labeling, and is highly helpful in discovering six additional novel SFAHFAs in the cecum and colon contents of WKAH/HKmSlc rats fed with normal and high-fat diet (HFD). The identified DMED labeled SFAHFAs were characterized by their detailed MS/MS analysis, and their plausible fragmentation patterns were proposed. The concentrations of SFAHFAs were significantly reduced in the cecum of HFD group compared to the control. Hence, the proposed method could be a promising tool to apply for the enhanced detection of SFAHFAs in various biological matrices, which in turn facilitate the understanding of their sources, and physiological functions of these novel lipids.
• Identification of short-chain fatty acid esters of hydroxy fatty acids (SFAHFAs) in a murine model by nontargeted analysis using ultra-high-performance liquid chromatography/linear ion trap quadrupole-Orbitrap mass spectrometry.
  Siddabasave Gowda B Gowda; Chongsheng Liang; Divyavani Gowda; Fengjue Hou; Kentaro Kawakami; Satoru Fukiya; Atsushi Yokota; Hitoshi Chiba; Shu-Ping Hui
  Rapid communications in mass spectrometry : RCM, 34, 17, e8831, 15 Sep. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, RATIONALE: Fatty acid esters of hydroxy fatty acids (FAHFAs) are recently discovered endogenous lipids with outstanding health benefits. FAHFAs are known to exhibit antioxidant, antidiabetic and anti-inflammatory properties. The number of known long-chain FAHFAs in mammalian tissues and dietary resources increased recently because of the latest developments in high-resolution tandem mass spectrometry techniques. However, there are no reports on the identification of short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs). METHODS: Intestinal contents, tissues, and plasma of rats fed with high-fat diet (HFD) and normal diet (ND) were analyzed for fatty acids, hydroxy fatty acids, and FAHFAs using ultra-high-performance liquid chromatography (UHPLC) and linear trap quadrupole-Orbitrap mass spectrometry (LTQ Orbitrap MS) with negative heated electrospray ionization. RESULTS: Untargeted analysis of total lipid extracts from murine samples (male 13-week-old WKAH/HKmSlc rats) led to the identification of several new SFAHFAs of acetic acid or propanoic acid esterified long-chain (>C20)-hydroxy fatty acids. Furthermore, MS3 analysis revealed the position of the hydroxyl group in the long-chain fatty acid as C-2. The relative amounts of SFAHFAs were quantified in intestinal contents and their tissues (Cecum, small intestine, and large intestine), liver, and plasma of rats fed with HFD and ND. The large intestine showed the highest abundance of SFAHFAs with a concentration range from 0.84 to 57 pmol/mg followed by the cecum with a range of 0.66 to 28.6 pmol/mg. The SFAHFAs were significantly altered between the HFD and ND groups, with a strong decreasing tendency under HFD conditions. CONCLUSIONS: Identification of these novel SFAHFAs can contribute to a better understanding of the chemical and biological properties of individual SFAHFAs and their possible sources in the gut, which in turn helps us tackle the role of these lipids in various metabolic diseases.
• Association between 12α-hydroxylated bile acids and hepatic steatosis in rats fed a high-fat diet.
  Shota Hori; Takayuki Abe; Dong Geun Lee; Satoru Fukiya; Atsushi Yokota; Nao Aso; Bungo Shirouchi; Masao Sato; Satoshi Ishizuka
  The Journal of nutritional biochemistry, 83, 108412, 108412, 20 May 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, High-fat (HF) diet induces hepatic steatosis that is a risk factor for noncommunicable diseases such as obesity, type 2 diabetes and cardiovascular disease. Previously, we found that HF feeding in rats increases the excretion of fecal bile acids (BAs), specifically 12α-hydroxylated (12αOH) BAs. Although the liver is the metabolic center in our body, the association between hepatic steatosis and 12αOH BAs in HF-fed rats is unclear. Thus, we investigated extensively BA composition in HF-fed rats and evaluated the association between hepatic steatosis and 12αOH BAs. Acclimated male inbred WKAH/HkmSlc rats were divided into two groups and fed either control or HF diet for 8 weeks. Feeding HF diet increased hepatic triglyceride and total cholesterol concentrations, which correlated positively with 12αOH BAs concentrations but not with non-12αOH BAs in the feces, portal plasma and liver. Accompanied by the increase in 12αOH BAs, the rats fed HF diet showed increased fat absorption and higher mRNA expression of liver Cidea. The enhancement of 12αOH BA secretion may contribute to hepatic steatosis by the promotion of dietary fat absorption and hepatic Cidea mRNA expression. The increase in 12αOH BAs was associated with enhanced liver cholesterol 7α-hydroxylase (Cyp7a1) and sterol 12α-hydroxylase (Cyp8b1) mRNA expression. There was a significant increase in 7α-hydroxycholesterol, a precursor of BAs, in the liver of HF-fed rats. Altogether, these data suggest that the HF diet increases preferentially 12αOH BAs synthesis by utilizing the accumulated hepatic cholesterol and enhancing mRNA expression of Cyp7a1 and Cyp8b1 in the liver.
• Application of Recombinase-Based In Vivo Expression Technology to Bifidobacterium longum subsp. longum for Identification of Genes Induced in the Gastrointestinal Tract of Mice.
  Hiroka Koguchi; Natsumi Ishigami; Mikiyasu Sakanaka; Kako Yoshida; Sayaka Hiratou; Mina Shimada; Satoru Fukiya; Kei Sonoyama; Atsushi Yokota
  Microorganisms, 8, 3, 13 Mar. 2020, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Bifidobacteria are one of the major components in human gut microbiota and well-known as beneficial microbes. However, clarification of commensal mechanisms of bifidobacteria in the intestines is still ongoing, especially in the presence of the gut microbiota. Here, we applied recombinase-based in vivo expression technology (R-IVET) using the bacteriophage P1 Cre/loxP system to Bifidobacterium longum subsp. longum 105-A (B. longum 105-A) to identify genes that are specifically expressed in the gastrointestinal tract of conventionally raised mice. Oral administration of the genomic DNA library of B. longum 105-A to conventionally raised mice resulted in the identification of 73 in vivo-induced genes. Four out of seven tested genes were verified in vivo-specific induction at least in the cecum by quantitative reverse transcription PCR. Although there is still room for improvement of the system, our findings can contribute to expanding our understanding of the commensal behavior of B. longum in the gut ecosystem.
• Untargeted lipidomic analysis of plasma from high fat diet-induced obese rats using UHPLC- linear trap quadrupole -Orbitrap MS.
  Siddabasave Gowda B Gowda; Zi-Jun Gao; Zhen Chen; Takayuki Abe; Shota Hori; Satoru Fukiya; Satoshi Ishizuka; Atsushi Yokota; Hitoshi Chiba; Shu-Ping Hui
  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 36, 7, 821, 828, 17 Jan. 2020, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, High-fat diet (HFD)-induced obesity is a primary risk factor for serious health problems. Although much research has been performed at the genomic level, lipidomic studies were limited. In this study, we aim to obtain a comprehensive profile of circulating plasma lipids, which are altered in rodent rat obesity by untargeted liquid chromatography-mass spectrometry. Rats fed with HFD for 8 weeks had increased body weight, liver and adipose tissue weight. The analysis results revealed that polyunsaturated fatty acids (PUFAs) and their corresponding phosphatidylcholine, phosphatidylinositol, and phosphatidylserine were significantly decreased in rats fed with HFD. In contrast, less unsaturated and ether type phosphatidylglycerols were increased. The triacylglycerides (TAGs) having saturated FA were increased in the HFD condition, whereas TAGs having PUFA were decreased. The levels of many plasma lipids were altered, and interestingly PUFA derived lipids were negatively associated with obesity. This signifies the importance of a PUFAs enriched diet to overwhelm obesity associated diseases.
• Administration of Cholic Acid Inhibits Equol Production from Daidzein in Mice.
  Hiroko Yoshioka; Masamichi Watanabe; Fumio Nanba; Toshio Suzuki; Satoru Fukiya; Atsushi Yokota; Toshiya Toda
  Journal of nutritional science and vitaminology, 66, 6, 571, 576, 2020, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Equol (Eq) is a metabolite of soy isoflavone daidzein (De) produced by the intestinal microbiota. The clinical effectiveness of soy isoflavone is considered to depend on the individual ability of Eq production. Previous studies have demonstrated that habitual dietary patterns may influence the production of Eq. For example, high Eq producers consumed less fat as a percentage of energy than low Eq producers. However, the inhibitory factors of Eq production are unknown. Recently, it was reported that bile acids induced by high-fat diet consumption may be a host-related factor controlling the composition of the intestinal microbiota. In this study, we investigated the effect of cholic acid (CA) administration, a mimic of the microbiota altered by a high-fat diet, on Eq production in mice. CA administration significantly decreased the levels of the De metabolites Eq, dihydrodaidzein, and O-desmethylangolensin in the serum of mice. However, CA administration did not affect the total molar concentration of De and its metabolites. Moreover, CA administration increased the levels of secondary bile acids, particularly deoxycholic acid (DCA), which has strong antibacterial activity in the cecum contents of mice. Thus, CA administration may increase the levels of DCA, a secondary bile acid, resulting in inhibition of Eq production. These findings may help to reveal the factors inhibiting Eq production and enhance the clinical effectiveness of isoflavone intake.
• Evolutionary adaptation in fucosyllactose uptake systems supports bifidobacteria-infant symbiosis.
  Mikiyasu Sakanaka; Morten Ejby Hansen; Aina Gotoh; Toshihiko Katoh; Keisuke Yoshida; Toshitaka Odamaki; Hiroyuki Yachi; Yuta Sugiyama; Shin Kurihara; Junko Hirose; Tadasu Urashima; Jin-Zhong Xiao; Motomitsu Kitaoka; Satoru Fukiya; Atsushi Yokota; Leila Lo Leggio; Maher Abou Hachem; Takane Katayama
  Science advances, 5, 8, eaaw7696, Aug. 2019, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
  English, Scientific journal, The human gut microbiota established during infancy has persistent effects on health. In vitro studies have suggested that human milk oligosaccharides (HMOs) in breast milk promote the formation of a bifidobacteria-rich microbiota in infant guts; however, the underlying molecular mechanism remains elusive. Here, we characterized two functionally distinct but overlapping fucosyllactose transporters (FL transporter-1 and -2) from Bifidobacterium longum subspecies infantis. Fecal DNA and HMO consumption analyses, combined with deposited metagenome data mining, revealed that FL transporter-2 is primarily associated with the bifidobacteria-rich microbiota formation in breast-fed infant guts. Structural analyses of the solute-binding protein (SBP) of FL transporter-2 complexed with 2'-fucosyllactose and 3-fucosyllactose, together with phylogenetic analysis of SBP homologs of both FL transporters, highlight a unique adaptation strategy of Bifidobacterium to HMOs, in which the gain-of-function mutations enable FL transporter-2 to efficiently capture major fucosylated HMOs. Our results provide a molecular insight into HMO-mediated symbiosis and coevolution between bifidobacteria and humans.
• Ingestion of difructose anhydride III partially suppresses the deconjugation and 7α-dehydroxylation of bile acids in rats fed with a cholic acid-supplemented diet.
  Dong Geun Lee; Shota Hori; Ohji Kohmoto; Shinri Kitta; Ryo Yoshida; Yasutake Tanaka; Hidehisa Shimizu; Keisuke Takahashi; Taizo Nagura; Hirokatsu Uchino; Satoru Fukiya; Atsushi Yokota; Satoshi Ishizuka
  Bioscience, biotechnology, and biochemistry, 83, 7, 1329, 1335, Jul. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Difructose anhydride III (DFAIII) is a prebiotic involved in the reduction of secondary bile acids (BAs). We investigated whether DFAIII modulates BA metabolism, including enterohepatic circulation, in the rats fed with a diet supplemented with cholic acid (CA), one of the 12α-hydroxylated BAs. After acclimation, the rats were fed with a control diet or a diet supplemented with DFAIII. After 2 weeks, each group was further divided into two groups and was fed diet with or without CA supplementation at 0.5 g/kg diet. BA levels were analyzed in aortic and portal plasma, liver, intestinal content, and feces. As a result, DFAIII ingestion reduced the fecal deoxycholic acid level via the partial suppression of deconjugation and 7α-dehydroxylation of BAs following CA supplementation. These results suggest that DFAIII suppresses production of deoxycholic acid in conditions of high concentrations of 12α-hydroxylated BAs in enterohepatic circulation, such as obesity or excess energy intake. Abbreviation: BA: bile acid; BSH: bile salt hydrolase; CA: cholic acid; DCA: deoxycholic acid; DFAIII: difructose anhydride III; MCA: muricholic acid; MS: mass spectrometry; NCDs: non-communicable diseases; LC: liquid chromatography; SCFA: short-chain fatty acid; TCA: taurocholic acid; TCDCA: taurochenodeoxycholic acid; TDCA: taurodeoxycholic acid; TUDCA: tauroursodeoxychlic acid; TαMCA: tauro-α-muricholic acid; TβMCA: tauro-β-muricholic acid; TωMCA: tauro-ω-muricholic acid.
• The membrane phospholipid cardiolipin plays a pivotal role in bile acid adaptation by Lactobacillus gasseri JCM1131T.
  Shinji Kato; Haruhi Tobe; Hiroki Matsubara; Mariko Sawada; Yasuko Sasaki; Satoru Fukiya; Naoki Morita; Atsushi Yokota
  Biochimica et biophysica acta. Molecular and cell biology of lipids, 1864, 3, 403, 412, Mar. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• A Transposon Mutagenesis System for Bifidobacterium longum subsp. longum Based on an IS3 Family Insertion Sequence, ISBlo11.
  Mikiyasu Sakanaka; Shingo Nakakawaji; Shin Nakajima; Satoru Fukiya; Arisa Abe; Wataru Saburi; Haruhide Mori; Atsushi Yokota
  Applied and environmental microbiology, 84, 17, 01 Sep. 2018, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Bifidobacteria are a major component of the intestinal microbiota in humans, particularly breast-fed infants. Therefore, elucidation of the mechanisms by which these bacteria colonize the intestine is desired. One approach is transposon mutagenesis, a technique currently attracting much attention because, in combination with next-generation sequencing, it enables exhaustive identification of genes that contribute to microbial fitness. We now describe a transposon mutagenesis system for Bifidobacterium longum subsp. longum 105-A (JCM 31944) based on ISBlo11, a native IS3 family insertion sequence. To build this system, xylose-inducible or constitutive bifidobacterial promoters were tested to drive the expression of full-length or a truncated form at the N terminus of the ISBlo11 transposase. An artificial transposon plasmid, pBFS12, in which ISBlo11 terminal inverted repeats are separated by a 3-bp spacer, was also constructed to mimic the transposition intermediate of IS3 elements. The introduction of this plasmid into a strain expressing transposase resulted in the insertion of the plasmid with an efficiency of >103 CFU/μg DNA. The plasmid targets random 3- to 4-bp sequences, but with a preference for noncoding regions. This mutagenesis system also worked at least in B. longum NCC2705. Characterization of a transposon insertion mutant revealed that a putative α-glucosidase mediates palatinose and trehalose assimilation, demonstrating the suitability of transposon mutagenesis for loss-of-function analysis. We anticipate that this approach will accelerate functional genomic studies of B. longum subsp. longumIMPORTANCE Several hundred species of bacteria colonize the mammalian intestine. However, the genes that enable such bacteria to colonize and thrive in the intestine remain largely unexplored. Transposon mutagenesis, combined with next-generation sequencing, is a promising tool to comprehensively identify these genes but has so far been applied only to a small number of intestinal bacterial species. In this study, a transposon mutagenesis system was established for Bifidobacterium longum subsp. longum, a representative health-promoting Bifidobacterium species. The system enables the identification of genes that promote colonization and survival in the intestine and should help illuminate the physiology of this species.
• Lipoteichoic acids are embedded in cell walls during logarithmic phase, but exposed on membrane vesicles in Lactobacillus gasseri JCM 1131T
  T. Shiraishi; S. Yokota; Y. Sato; T. Ito; S. Fukiya; S. Yamamoto; T. Sato; A. Yokota
  Beneficial Microbes, 9, 4, 653, 662, Wageningen Academic Publishers, 2018, [Peer-reviewed]
  English, Scientific journal
• Isolation of six novel 7-oxo- or urso-type secondary bile acid-producing bacteria from rat cecal contents.
  Sarinya Tawthep; Satoru Fukiya; Ja-Young Lee; Masahito Hagio; Yoshitoshi Ogura; Tetsuya Hayashi; Atsushi Yokota
  Journal of bioscience and bioengineering, 124, 5, 514, 522, Nov. 2017, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Bifidobacterium bifidum Extracellular Sialidase Enhances Adhesion to the Mucosal Surface and Supports Carbohydrate Assimilation.
  Keita Nishiyama; Yuji Yamamoto; Makoto Sugiyama; Takashi Takaki; Tadasu Urashima; Satoru Fukiya; Atsushi Yokota; Nobuhiko Okada; Takao Mukai
  mBio, 8, 5, 03 Oct. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Comprehensive evaluation of the bactericidal activities of free bile acids in the large intestine of humans and rodents.
  Masamichi Watanabe; Satoru Fukiya; Atsushi Yokota
  Journal of lipid research, 58, 6, 1143, 1152, Jun. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Pyruvate dehydrogenase complex regulator (PdhR) gene deletion boosts glucose metabolism in Escherichia coli under oxygen-limited culture conditions.
  Soya Maeda; Kumiko Shimizu; Chie Kihira; Yuki Iwabu; Ryuichi Kato; Makoto Sugimoto; Satoru Fukiya; Masaru Wada; Atsushi Yokota
  Journal of bioscience and bioengineering, 123, 4, 437, 443, Apr. 2017, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Characteristics of lactic acid bacteria from the view of the structural diversity of lipoteichoic acids
  白石 宗; 横田 伸一; 吹谷 智; 横田 篤
  JATAFFジャーナル = JATAFF journal : 農林水産技術, 5, 3, 26, 32, 農林水産・食品産業技術振興協会, Mar. 2017
  Japanese
• Development of practical gene-mutagenesis systems in bifidobacteria
  Fukiya Satoru
  Seibutsu-kogaku Kaishi, 94, 3, 110, 116, 公益社団法人日本生物工学会, 25 Mar. 2016, [Invited], [Lead author, Last author, Corresponding author]
  Japanese
• Lactobacillus rhamnosus GG increases Toll-like receptor 3 gene expression in murine small intestine ex vivo and in vivo
  A. Aoki-Yoshida; S. Saito; S. Fukiya; R. Aoki; Y. Takayama; C. Suzuki; K. Sonoyama
  BENEFICIAL MICROBES, 7, 3, 421, 429, 2016, [Peer-reviewed]
  English, Scientific journal
• Structural diversity and biological significance of lipoteichoic acid in Gram-positive bacteria: focusing on beneficial probiotic lactic acid bacteria.
  Tsukasa Shiraishi; Shinichi Yokota; Satoru Fukiya; Atsushi Yokota
  Bioscience of microbiota, food and health, 35, 4, 147, 161, 2016, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Bacterial cell surface molecules are at the forefront of host-bacterium interactions. Teichoic acids are observed only in Gram-positive bacteria, and they are one of the main cell surface components. Teichoic acids play important physiological roles and contribute to the bacterial interaction with their host. In particular, lipoteichoic acid (LTA) anchored to the cell membrane has attracted attention as a host immunomodulator. Chemical and biological characteristics of LTA from various bacteria have been described. However, most of the information concerns pathogenic bacteria, and information on beneficial bacteria, including probiotic lactic acid bacteria, is insufficient. LTA is structurally diverse. Strain-level structural diversity of LTA is suggested to underpin its immunomodulatory activities. Thus, the structural information on LTA in probiotics, in particular strain-associated diversity, is important for understanding its beneficial roles associated with the modulation of immune response. Continued accumulation of structural information is necessary to elucidate the detailed physiological roles and significance of LTA. In this review article, we summarize the current state of knowledge on LTA structure, in particular the structure of LTA from lactic acid bacteria. We also describe the significance of structural diversity and biological roles of LTA.
• Isolation and transposition properties of ISBlo11, an active insertion sequence belonging to the IS3 family, from Bifidobacterium longum 105-A.
  Mikiyasu Sakanaka; Satoru Fukiya; Ryoko Kobayashi; Arisa Abe; Yosuke Hirayama; Yasunobu Kano; Atsushi Yokota
  FEMS microbiology letters, 362, 7, Apr. 2015, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Transposon mutagenesis systems are still under development in bifidobacteria, partly because intrinsic active insertion sequences are not well characterized in bifidobacteria. Here, we isolated an active insertion sequence, ISBlo11, from Bifidobacterium longum 105-A using a sacB-based counterselection system, which is generally used to screen for active insertion sequences from bacterial genomes. ISBlo11 is 1432 bp long and belongs to the IS3 family. It has a single ORF encoding a transposase and 25-bp inverted repeats at its termini. Full-length copies of ISBlo11 are specifically conserved among certain B. longum genomes and exist in different sites. Transposition analysis of an artificial ISBlo11 transposon using an Escherichia coli conjugation system revealed that ISBlo11 has adequate transposition activity, comparable to the reported activity of IS629, another IS3 family element initially isolated from Shigella sonnei. ISBlo11 also showed low transposition selectivity for non-conserved 3- or 4-bp target sequences. These characteristics of ISBlo11 seem suitable for the development of a new transposon mutagenesis system in bifidobacteria.
• Mouse models for assessing the cross-protective efficacy of oral non-typhoidal Salmonella vaccine candidates harbouring in-frame deletions of the ATP-dependent protease lon and other genes.
  Hidenori Matsui; Satoru Fukiya; Chie Kodama-Akaboshi; Masahiro Eguchi; Tomoko Yamamoto
  Journal of medical microbiology, 64, Pt 3, 295, 302, Mar. 2015, [Peer-reviewed], [International Magazine]
  English, In BALB/c mouse models of Salmonella enterica serovar Typhimurium infection, a single oral immunization with a mutant strain with an insertion of the chloramphenicol resistance gene into the ATP-dependent protease clpP or lon gene decreased the number of salmonellae in each tissue sample 5 days after oral challenge with virulent S. Typhimurium at weeks 26 and 54 post-immunization. These data suggested that an oral immunization with the ClpP- or Lon-disrupted S. Typhimurium strain could provide long-term protection against oral challenge with virulent S. Typhimurium. Accordingly, recombinant oral non-typhoidal Salmonella (NTS) vaccines were constructed by incorporating mutants of both S. Typhimurium and S. enterica serovar Enteritidis harbouring stable in-frame markerless deletions of the clpP-lon-sulA (suppressor of lon), lon-sulA or lon-msbB (acyltransferase) genes. Amongst these orally administered vaccine candidates, those with the lon-sulA gene deletion mutants of S. Typhimurium and S. Enteritidis protected BALB/c and C57BL/6J mice against oral challenge with both virulent S. Typhimurium and virulent S. Enteritidis. Therefore, the in-frame markerless lon-sulA gene deletion mutant of S. Typhimurium or S. Enteritidis could be a promising cross-protective NTS live vaccine candidate for practical use in humans.
• Diet supplementation with cholic acid promotes intestinal epithelial proliferation in rats exposed to γ-radiation.
  Masahito Hagio; Hidehisa Shimizu; Ga-Hyun Joe; Manami Takatsuki; Maiko Shiwaku; Hong Xu; Ja-Young Lee; Nobuyuki Fujii; Satoru Fukiya; Hiroshi Hara; Atsushi Yokota; Satoshi Ishizuka
  Toxicology letters, 232, 1, 246, 52, 05 Jan. 2015, [Peer-reviewed], [International Magazine]
  English, Consumption of a high-fat diet increases some secondary bile acids (BAs) such as deoxycholic acid (DCA) in feces. DCA is derived from cholic acid (CA), a primary BA. We evaluated intestinal epithelial proliferation and BA metabolism in response to oral administration of cholic acid (CA) in rats to determine the influence of a CA diet on the responses of gut epithelia to γ-rays. WKAH/HkmSlc rats were divided into two dietary groups: control diet or CA-supplemented (2g/kg diet) diet. Some of the rats from each group were irradiated with γ-rays, and epithelial cell proliferation in the colon was analyzed histochemically. Unirradiated CA-fed rats had high levels of DCA and CA in the sera, as well as the presence of taurocholic acid in their feces. Significant increases were observed in both epithelial proliferation and the number of epithelial cells in the colon of the CA-fed rats, and this effect was observed at 8 weeks after γ-ray exposure. Furthermore, extracts from both cecal contents and sera of the unirradiated CA-fed rats promoted proliferation of IEC-6 cells. These results indicate that BAs in enterohepatic circulation promote proliferation and survival of the intestinal epithelium after receiving DNA damage.
• Activity of ERK regulates mucin 3 expression and is involved in undifferentiated Caco-2 cell death induced by 3-oxo-C12-homoserine lactone.
  Hidehisa Shimizu; Nanako Baba; Takuma Nose; Ryoko Taguchi; Shinya Tanaka; Ga-Hyun Joe; Hideaki Maseda; Nobuhiko Nomura; Masahito Hagio; Ja-Young Lee; Satoru Fukiya; Atsushi Yokota; Satoshi Ishizuka; Hitoshi Miyazaki
  Bioscience, biotechnology, and biochemistry, 79, 6, 937, 42, 2015, [Peer-reviewed], [International Magazine]
  English, The signal molecule, 3-oxo-C12-homoserine lactone (3-oxo-C12-HSL), is similar to a mammalian hormone in bacteria. Although most studies have examined the effects of high 3-oxo-C12-HSL concentrations (>200 μM) on mammalian cellular functions because ~600 μM 3-oxo-C12-HSL can be secreted in biofilms of Pseudomonas aeruginosa grown in vitro, we previously showed that a low 3-oxo-C12-HSL concentration (30 μM) induces the apoptosis of undifferentiated Caco-2 cells through suppressing Akt activity. Here, we found that a low concentration of 3-oxo-C12-HSL-activated ERK1/2 in undifferentiated Caco-2 cells. Incubating cells with the ERK pathway inhibitor U0126 for 30 min alleviated the mucin 3 (MUC3) expression suppressed by 3-oxo-C12-HSL, and the upregulation of MUC3 expression induced by a 48-h incubation with U0126-reduced cell death. Thus, altered MUC3 expression caused by long-term attenuated ERK1/2 activity might correlate with the death of undifferentiated Caco-2 cells induced by 3-oxo-C12-HSL.
• Complete Genome Sequence of Bifidobacterium longum 105-A, a Strain with High Transformation Efficiency.
  Yu Kanesaki; Hisayoshi Masutani; Mikiyasu Sakanaka; Yuh Shiwa; Takatomo Fujisawa; Yasukazu Nakamura; Atsushi Yokota; Satoru Fukiya; Tohru Suzuki; Hirofumi Yoshikawa
  Genome announcements, 2, 6, 18 Dec. 2014, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Bifidobacterium longum 105-A shows high transformation efficiency and allows for the generation of gene knockout mutants through homologous recombination. Here, we report the complete genome sequence of strain 105-A. Genes encoding at least four putative restriction-modification systems were found in this genome, which might contribute to its transformation efficiency.
• Functional analysis of bifidobacterial promoters in Bifidobacterium longum and Escherichia coli using the α-galactosidase gene as a reporter.
  Mikiyasu Sakanaka; Saki Tamai; Yosuke Hirayama; Ai Onodera; Hiroka Koguchi; Yasunobu Kano; Atsushi Yokota; Satoru Fukiya
  Journal of bioscience and bioengineering, 118, 5, 489, 95, Nov. 2014, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
  English, Scientific journal
• Deoxycholic acid is involved in the proliferation and migration of vascular smooth muscle cells.
  Hidehisa Shimizu; Masahito Hagio; Hitoshi Iwaya; Ikuya Tsuneki; Ja-Young Lee; Satoru Fukiya; Atsushi Yokota; Hitoshi Miyazaki; Hiroshi Hara; Satoshi Ishizuka
  Journal of nutritional science and vitaminology, 60, 6, 450, 4, 2014, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Characterization of a Lactobacillus gasseri JCM 1131(T) Lipoteichoic Acid with a Novel Glycolipid Anchor Structure (vol 79, pg 3315, 2013)
  Tsukasa Shiraishi; Shin-ichi Yokota; Naoki Morita; Satoru Fukiya; Satoru Tomita; Naoto Tanaka; Sanae Okada; Atsushi Yokota
  APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79, 24, 7931, 7931, Dec. 2013, [Peer-reviewed]
  English
• Contribution of the 7β-hydroxysteroid dehydrogenase from Ruminococcus gnavus N53 to ursodeoxycholic acid formation in the human colon.
  Ja-Young Lee; Hisashi Arai; Yusuke Nakamura; Satoru Fukiya; Masaru Wada; Atsushi Yokota
  Journal of lipid research, 54, 11, 3062, 9, 11, Nov. 2013, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Bile acid composition in the colon is determined by bile acid flow in the intestines, the population of bile acid-converting bacteria, and the properties of the responsible bacterial enzymes. Ursodeoxycholic acid (UDCA) is regarded as a chemopreventive beneficial bile acid due to its low hydrophobicity. However, it is a minor constituent of human bile acids. Here, we characterized an UDCA-producing bacterium, N53, isolated from human feces. 16S rDNA sequence analysis identified this isolate as Ruminococcus gnavus, a novel UDCA-producer. The forward reaction that produces UDCA from 7-oxo-lithocholic acid was observed to have a growth-dependent conversion rate of 90-100% after culture in GAM broth containing 1 mM 7-oxo-lithocholic acid, while the reverse reaction was undetectable. The gene encoding 7β-hydroxysteroid dehydrogenase (7β-HSDH), which facilitates the UDCA-producing reaction, was cloned and overexpressed in Escherichia coli. Characterization of the purified 7β-HSDH revealed that the kcat/Km value was about 55-fold higher for the forward reaction than for the reverse reaction, indicating that the enzyme favors the UDCA-producing reaction. As R. gnavus is a common, core bacterium of the human gut microbiota, these results suggest that this bacterium plays a pivotal role in UDCA formation in the colon.
• Lacto-N-biosidase encoded by a novel gene of Bifidobacterium longum subspecies longum shows unique substrate specificity and requires a designated chaperone for its active expression.
  Haruko Sakurama; Masashi Kiyohara; Jun Wada; Yuji Honda; Masanori Yamaguchi; Satoru Fukiya; Atsushi Yokota; Hisashi Ashida; Hidehiko Kumagai; Motomitsu Kitaoka; Kenji Yamamoto; Takane Katayama
  The Journal of biological chemistry, 288, 35, 25194, 206, 35, 30 Aug. 2013, [Peer-reviewed], [International Magazine]
  English, Infant gut-associated bifidobacteria possess species-specific enzymatic sets to assimilate human milk oligosaccharides, and lacto-N-biosidase (LNBase) is a key enzyme that degrades lacto-N-tetraose (Galβ1-3GlcNAcβ1-3Galβ1-4Glc), the main component of human milk oligosaccharides, to lacto-N-biose I (Galβ1-3GlcNAc) and lactose. We have previously identified LNBase activity in Bifidobacterium bifidum and some strains of Bifidobacterium longum subsp. longum (B. longum). Subsequently, we isolated a glycoside hydrolase family 20 (GH20) LNBase from B. bifidum; however, the genome of the LNBase(+) strain of B. longum contains no GH20 LNBase homolog. Here, we reveal that locus tags BLLJ_1505 and BLLJ_1506 constitute LNBase from B. longum JCM1217. The gene products, designated LnbX and LnbY, respectively, showed no sequence similarity to previously characterized proteins. The purified enzyme, which consisted of LnbX only, hydrolyzed via a retaining mechanism the GlcNAcβ1-3Gal linkage in lacto-N-tetraose, lacto-N-fucopentaose I (Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1-4Glc), and sialyllacto-N-tetraose a (Neu5Acα2-3Galβ1-3GlcNAcβ1-3Galβ1-4Gal); the latter two are not hydrolyzed by GH20 LNBase. Among the chromogenic substrates examined, the enzyme acted on p-nitrophenyl (pNP)-β-lacto-N-bioside I (Galβ1-3GlcNAcβ-pNP) and GalNAcβ1-3GlcNAcβ-pNP. GalNAcβ1-3GlcNAcβ linkage has been found in O-mannosyl glycans of α-dystroglycan. Therefore, the enzyme may serve as a new tool for examining glycan structures. In vitro refolding experiments revealed that LnbY and metal ions (Ca(2+) and Mg(2+)) are required for proper folding of LnbX. The LnbX and LnbY homologs have been found only in B. bifidum, B. longum, and a few gut microbes, suggesting that the proteins have evolved in specialized niches.
• Characterization of a Lactobacillus gasseri JCM 1131T lipoteichoic acid with a novel glycolipid anchor structure.
  Tsukasa Shiraishi; Shin-ichi Yokota; Naoki Morita; Satoru Fukiya; Satoru Tomita; Naoto Tanaka; Sanae Okada; Atsushi Yokota
  Applied and environmental microbiology, 79, 10, 3315, 8, 10, May 2013, [Peer-reviewed], [International Magazine]
  English, We determined the chemical structure of lipoteichoic acid (LTA) from Lactobacillus gasseri JCM 1131(T). The repeating unit was comprised of glycerolphosphate and 2-alanylglycerolphosphate. The glycolipid anchor was tetrahexosylglycerol with two or three acyl groups. To our knowledge, this is the first demonstration of a tetrahexose structure in an LTA glycolipid anchor.
• Image analyzing method to evaluate in situ bioluminescence from an obligate anaerobe cultivated under various dissolved oxygen concentrations.
  Kazuaki Ninomiya; Ryuji Yamada; Masami Matsumoto; Satoru Fukiya; Takane Katayama; Chiaki Ogino; Nobuaki Shimizu
  Journal of bioscience and bioengineering, 115, 2, 196, 9, 2, Feb. 2013, [Peer-reviewed], [Domestic magazines]
  English, An image analyzing method was developed to evaluate in situ bioluminescence expression, without exposing the culture sample to the ambient oxygen atmosphere. Using this method, we investigated the effect of dissolved oxygen concentration on bioluminescence from an obligate anaerobe Bifidobacterium longum expressing bacterial luciferase which catalyzes an oxygen-requiring bioluminescent reaction.
• Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation.
  Yosuke Hirayama; Mikiyasu Sakanaka; Hidenori Fukuma; Hiroki Murayama; Yasunobu Kano; Satoru Fukiya; Atsushi Yokota
  Applied and environmental microbiology, 78, 14, 4984, 94, 14, Jul. 2012, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Functional analysis of Bifidobacterium genes is essential for understanding host-Bifidobacterium interactions with beneficial effects on human health; however, the lack of an effective targeted gene inactivation system in bifidobacteria has prevented the development of functional genomics in this bacterium. Here, we report the development of a markerless gene deletion system involving a double crossover in Bifidobacterium longum. Incompatible plasmid vectors were used to facilitate a second crossover step. The conditional replication vector pBS423-ΔrepA, which lacks the plasmid replication gene repA, was integrated into the target gene by a first crossover event. Subsequently, the replicative plasmid pTBR101-CM, which harbors repA, was introduced into this integrant to facilitate the second crossover step and subsequent elimination of the excised conditional replication vector from the cells by plasmid incompatibility. The proposed system was confirmed to work as expected in B. longum 105-A using the chromosomal full-length β-galactosidase gene as a target. Markerless gene deletion was tested using the aga gene, which encodes α-galactosidase, whose substrates include raffinose. Almost all the pTBR101-CM-transformed strains became double-crossover recombinants after subculture, and 4 out of the 270 double-crossover recombinants had lost the ability to assimilate raffinose. Genotype analysis of these strains revealed markerless gene deletion of aga. Carbohydrate assimilation analysis and α-galactosidase activity measurement were conducted using both the representative mutant and a plasmid-based aga-complemented strain. These functional analyses revealed that aga is the only gene encoding a functional α-galactosidase enzyme in B. longum 105-A.
• Alterations of glucose metabolism in Escherichia coli mutants defective in respiratory-chain enzymes.
  Chie Kihira; Yukari Hayashi; Naoki Azuma; Sakiko Noda; Soya Maeda; Satoru Fukiya; Masaru Wada; Kazunobu Matsushita; Atsushi Yokota
  Journal of biotechnology, 158, 4, 215, 23, 30 Apr. 2012, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The effects of reduced efficiency of proton-motive force (pmf) generation on glucose metabolism were investigated in Escherichia coli respiratory-chain mutants. The respiratory chain of E. coli consists of two NADH dehydrogenases and three terminal oxidases, all with different abilities to generate a pmf. The genes for isozymes with the highest pmf-generating capacity (NADH dehydrogenase-1 and cytochrome bo₃ oxidase) were knocked out singly or in combination, using a wild-type strain as the parent. Analyses of glucose metabolism by jar-fermentation revealed that the glucose consumption rate per cell increased with decreasing efficiency of pmf generation, as determined from the growth parameters of the mutants. The highest rate of glucose metabolism was observed in the double mutant, and the lowest was observed in the wild-type strain. The respiration rates of the single-knockout mutants were comparable to that of the wild-type strain, and that of the double mutant was higher, apparently as a result of the upregulation of the remaining respiratory chain enzymes. All of the strains excreted 2-oxoglutaric acid as a product of glucose metabolism. Additionally, all of the mutants excreted pyruvic acid and/or acetic acid. Interestingly, the double mutant excreted L-glutamic acid. Alterations of the fermentation profiles provide clues regarding the metabolic regulation in each mutant.
• Technological advances in bifidobacterial molecular genetics: application to functional genomics and medical treatments.
  Satoru Fukiya; Yosuke Hirayama; Mikiyasu Sakanaka; Yasunobu Kano; Atsushi Yokota
  Bioscience of microbiota, food and health, 31, 2, 15, 25, 2012, [Peer-reviewed], [Lead author, Corresponding author], [Domestic magazines]
  English, Bifidobacteria are well known as beneficial intestinal bacteria that exert health-promoting effects in humans. In addition to physiological and immunological investigations, molecular genetic technologies have been developed and have recently started to be applied to clarify the molecular bases of host-Bifidobacterium interactions. These technologies include transformation technologies and Escherichia coli-Bifidobacterium shuttle vectors that enable heterologous gene expression. In this context, a plasmid artificial modification method that protects the introduced plasmid from the restriction system in host bifidobacteria has recently been developed to increase transformation efficiency. On the other hand, targeted gene inactivation systems, which are vital for functional genomics, seemed far from being practically applicable in bifidobacteria. However, remarkable progress in this technology has recently been achieved, enabling functional genomics in bifidobacteria. Integrated use of these molecular genetic technologies with omics-based analyses will surely boost characterization of the molecular basis underlying beneficial effects of bifidobacteria. Applications of recombinant bifidobacteria to medical treatments have also progressed.
• Is bile acid a determinant of the gut microbiota on a high-fat diet?
  Atsushi Yokota; Satoru Fukiya; Tadasuke Ooka; Yoshitoshi Ogura; Tetsuya Hayashi; Satoshi Ishizuka
  Gut Microbes, 3, 5, 455, 459, Landes Bioscience, 2012, [Peer-reviewed]
  English, Scientific journal
• Bile acid is a host factor that regulates the composition of the cecal microbiota in rats.
  K B M Saiful Islam; Satoru Fukiya; Masahito Hagio; Nobuyuki Fujii; Satoshi Ishizuka; Tadasuke Ooka; Yoshitoshi Ogura; Tetsuya Hayashi; Atsushi Yokota
  Gastroenterology, 141, 5, 1773, 81, Nov. 2011, [Peer-reviewed], [Lead author], [International Magazine]
  English, Scientific journal
• Ingestion of epilactose, a non-digestible disaccharide, improves postgastrectomy osteopenia and anemia in rats through the promotion of intestinal calcium and iron absorption.
  Takuya Suzuki; Megumi Nishimukai; Aki Shinoki; Hidenori Taguchi; Satoru Fukiya; Atsushi Yokota; Wataru Saburi; Takeshi Yamamoto; Hiroshi Hara; Hirokazu Matsui
  Journal of agricultural and food chemistry, 58, 19, 10787, 92, 13 Oct. 2010, [Peer-reviewed], [International Magazine]
  English, Gastrectomy often results in osteopenia and anemia because of calcium (Ca) and iron (Fe) malabsorption. Here, we investigated the effects of feeding epilactose, a non-digestible disaccharide, on gastrectomy-induced osteopenia, anemia, and Ca and Fe malabsorption in male Sprague-Dawley rats. Totally gastrectomized or sham-operated rats were fed the control or epilactose (50 g/kg) diets for 30 days. Gastrectomy severely decreased intestinal Ca and Fe absorption, femoral bone strength, Ca content, hemoglobin concentration, and hematocrit. These decreases were partly or totally restored by feeding epilactose. Feeding epilactose increased the cecal tissue weight and the soluble Ca concentration and short-chain fatty acid pools of the cecal contents. Collectively, the increases in cecal mucosal area and/or soluble Ca concentration of the cecal contents, resulting from short-chain fatty acid production by intestinal microbes, are thought to be responsible for the epilactose-mediated promotion of Ca and Fe absorption in the gastrectomized rats.
• Characterization of an insertion sequence-like element, ISBlo15, identified in a size-increased cryptic plasmid pBK283 in Bifidobacterium longum BK28.
  Satoru Fukiya; Tomohiko Sugiyama; Yasunobu Kano; Atsushi Yokota
  Journal of bioscience and bioengineering, 110, 2, 141, 6, 2, Aug. 2010, [Peer-reviewed], [Lead author, Corresponding author], [Domestic magazines]
  English, The characteristics of mobile genetic elements in bifidobacteria are not well understood. We characterized an insertion sequence-like element of the IS200/IS605 family found in a size-increased cryptic plasmid in Bifidobacterium longum. During a plasmid profile analysis of B. longum BK strains, we encountered a 6.5-kbp cryptic plasmid pBK283 in B. longum BK28, the size of which has not been identified in bifidobacteria. Nucleotide sequence analysis indicated that an insertion sequence-like element was inserted into the 5.0-kbp pKJ50-like plasmid and resulted in a size increase of pBK283. The element, named ISBlo15, was 1593 bp in length and contained a single ORF encoding a putative transposase, which is similar to the transposase OrfB encoded by IS200/IS605 family elements. Several sequence characteristics, including conserved transposase motifs in OrfB and terminal palindromic sequences that differ from the typical terminal inverted repeats, strongly suggested that ISBlo15 is a member of the IS200/IS605 family. Sequences similar to ISBlo15 were widely distributed among the nine Bifidobacterium species tested, and those of highly homologous sequences were detected only in Bifidobacterium gallicum JCM8224(T).
• Molecular cloning and characterization of the AVR-Pia locus from a Japanese field isolate of Magnaporthe oryzae.
  Shinsuke Miki; Kotaro Matsui; Hideki Kito; Keisuke Otsuka; Taketo Ashizawa; Nobuko Yasuda; Satoru Fukiya; Junko Sato; Kazuyuki Hirayae; Yoshikatsu Fujita; Toshihiko Nakajima; Fusao Tomita; Teruo Sone
  Molecular plant pathology, 10, 3, 361, 74, May 2009, [Peer-reviewed], [International Magazine]
  English, Scientific journal, In order to clone and analyse the avirulence gene AVR-Pia from Japanese field isolates of Magnaporthe oryzae, a mutant of the M. oryzae strain Ina168 was isolated. This mutant, which was named Ina168m95-1, gained virulence towards the rice cultivar Aichi-asahi, which contains the resistance gene Pia. A DNA fragment (named PM01) that was deleted in the mutant and that co-segregated with avirulence towards Aichi-asahi was isolated. Three cosmid clones that included the regions that flanked PM01 were isolated from a genomic DNA library. One of these clones (46F3) complemented the mutant phenotype, which indicated clearly that this clone contained the avirulence gene AVR-Pia. Clone 46F3 contained insertions of transposable elements. The 46F3 insert was divided into fragments I-VI, and these were cloned individually into a hygromycin-resistant vector for the transformation of the mutant Ina168m95-1. An inoculation assay of the transformants revealed that fragment V (3.5 kb) contained AVR-Pia. By deletion analysis of fragment V, AVR-Pia was localized to an 1199-bp DNA fragment, which included a 255-bp open reading frame with weak homology to a bacterial cytochrome-c-like protein. Restriction fragment length polymorphism analysis of this region revealed that this DNA sequence co-segregated with the AVR-Pia locus in a genetic map that was constructed using Chinese isolates.
• Conversion of cholic acid and chenodeoxycholic acid into their 7-oxo derivatives by Bacteroides intestinalis AM-1 isolated from human feces.
  Satoru Fukiya; Miki Arata; Hiroko Kawashima; Daisuke Yoshida; Maki Kaneko; Kimiko Minamida; Jun Watanabe; Yoshio Ogura; Kiyohisa Uchida; Kikuji Itoh; Masaru Wada; Susumu Ito; Atsushi Yokota
  FEMS microbiology letters, 293, 2, 263, 70, 2, Apr. 2009, [Peer-reviewed], [Lead author], [International Magazine]
  English, Scientific journal, Secondary bile acid-producing bacteria were isolated from human feces to improve our appreciation of the functional diversity and redundancy of the intestinal microbiota. In total, 619 bacterial colonies were isolated using a nutrient-poor agar medium and the level of secondary bile acid formation was examined in each by a liquid culture, followed by thin-layer chromatography. Of five strains analyzed by 16S rRNA gene sequencing and biochemical testing, one was identified as Bacteroides intestinalis AM-1, which was not previously recognized as a secondary bile-acid producer. GC-MS revealed that B. intestinalis AM-1 converts cholic acid (CA) and chenodeoxycholic acid into their 7-oxo derivatives, 7-oxo-deoxycholic acid (7-oxo-DCA) and 7-oxo-lithocholic acid, respectively. Thus, B. intestinalis AM-1 possesses 7alpha-hydroxysteroid dehydrogenase (7alpha-HSDH) activity. In liquid culture, B. intestinalis AM-1 showed a relatively higher productivity of 7-oxo-DCA than Escherichia coli HB101 and Bacteroides fragilis JCM11019(T), which are known to possess 7alpha-HSDH activity. The level of 7alpha-HSDH activity was higher in B. intestinalis AM-1 than in the other two strains under the conditions tested. The 7alpha-HSDH activity in each of the three strains is not induced by CA; instead, it is regulated in a growth phase-dependent manner.
• Identification of the cellobiose 2-epimerase gene in the genome of Bacteroides fragilis NCTC 9343.
  Takeshi Senoura; Hidenori Taguchi; Shigeaki Ito; Shigeki Hamada; Hirokazu Matsui; Satoru Fukiya; Atsushi Yokota; Jun Watanabe; Jun Wasaki; Susumu Ito
  Bioscience, biotechnology, and biochemistry, 73, 2, 400, 6, 2, Feb. 2009, [Peer-reviewed], [International Magazine]
  English, Cellobiose 2-epimerase (CE, EC 5.1.3.11) catalyzes the reversible epimerization of cellobiose to 4-O-beta-D-glucopyranosyl-D-mannose. In this study, we found a CE gene in the genome sequence of non-cellulolytic Bacteroides fragilis NCTC 9343. The recombinant enzyme, expressed in Escherichia coli cells, catalyzed a hydroxyl stereoisomerism at the C-2 positions of the reducing terminal glucose and at the mannose moiety of cello-oligosaccharides, lactose, beta-mannobiose (4-O-beta-D-mannopyranosyl-D-mannose), and globotriose [O-alpha-D-galactopyranosyl-(1-->4)-O-beta-D-galactopyranosyl-(1-->4)-D-glucose]. The CE from B. fragilis showed less than 40% identity to reported functional CEs. It exhibited 44-63% identities to N-acyl-D-glucosamine 2-epimerase-like hypothetical proteins of unknown function in bacterial genome sequences of the phyla Firmicutes, Bacteroidetes, Proteobacteria, Chloroflexi, and Verrucomicrobia. On the other hand, it showed less than 26% identity to functional N-acyl-D-glucosamine 2-epimerases. Based on the amino acid homology and phylogenetic positions of the functional epimerases, we emphasize that many genes for putative N-acyl-D-glucosamine 2-epimerases and related hypothetical proteins of unknown function reported to date in the bacterial genomes should be annotated as CE-like proteins or putative CEs.
• [FISH-flow cytometry, a new tool for the analysis of intestinal microbiota].
  Satoru Fukiya; Atsushi Yokota
  Seikagaku. The Journal of Japanese Biochemical Society, 80, 5, 421, 5, 5, May 2008, [Invited], [Domestic magazines]
  Japanese
• Population dynamics of Bifidobacterium species in human feces during raffinose administration monitored by fluorescence in situ hybridization-flow cytometry.
  Achmad Dinoto; Tatiana M Marques; Kanta Sakamoto; Satoru Fukiya; Jun Watanabe; Susumu Ito; Atsushi Yokota
  Applied and environmental microbiology, 72, 12, 7739, 47, 12, Dec. 2006, [Peer-reviewed], [International Magazine]
  English, The population dynamics of bifidobacteria in human feces during raffinose administration were investigated at the species level by using fluorescence in situ hybridization (FISH) coupled with flow cytometry (FCM) analysis. Although double-staining FISH-FCM using both fluorescein isothiocyanate (FITC) and indodicarbocyanine (Cy5) as labeling dyes for fecal samples has been reported, the analysis was interfered with by strong autofluorescence at the FITC fluorescence region because of the presence of autofluorescence particles/debris in the fecal samples. We circumvented this problem by using only Cy5 fluorescent dye in the FISH-FCM analysis. Thirteen subjects received 2 g of raffinose twice a day for 4 weeks. Fecal samples were collected, and the bifidobacterial populations were monitored using the established FISH-FCM method. The results showed an increase in bifidobacteria from about 12.5% of total bacteria in the prefeeding period to about 28.7 and 37.2% after the 2-week and 4-week feeding periods, respectively. Bifidobacterium adolescentis, the Bifidobacterium catenulatum group, and Bifidobacterium longum were the major species, in that order, at the prefeeding period, and these bacteria were found to increase nearly in parallel during the raffinose administration. During the feeding periods, indigenous bifidobacterial populations became more diverse, such that minor species in human adults, such as Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium dentium, and Bifidobacterium angulatum, proliferated. Four weeks after raffinose administration was stopped, the proportion of each major bifidobacterial species, as well as that of total bifidobacteria, returned to approximately the original values for the prefeeding period, whereas that of each minor species appeared to differ considerably from its original value. To the best of our knowledge, these results provide the first clear demonstration of the population dynamics of indigenous bifidobacteria at the species level in response to raffinose administration.
• FISH-Flow Cytometry : A High-Throughput Molecular Ecological Analysis of Intestinal Microbiota
  DINOTO Achmad; FUKIYA Satoru; YOKOTA Atsushi
  Japanese Journal of Lactic Acid Bacteria, 17, 2, 110, 117, 日本乳酸菌学会, 01 Dec. 2006, [Invited]
  English
• Extensive genomic diversity in pathogenic Escherichia coli and Shigella Strains revealed by comparative genomic hybridization microarray.
  Satoru Fukiya; Hiroshi Mizoguchi; Toru Tobe; Hideo Mori
  Journal of bacteriology, 186, 12, 3911, 21, 12, Jun. 2004, [Peer-reviewed], [Lead author], [International Magazine]
  English, Escherichia coli, including the closely related genus Shigella, is a highly diverse species in terms of genome structure. Comparative genomic hybridization (CGH) microarray analysis was used to compare the gene content of E. coli K-12 with the gene contents of pathogenic strains. Missing genes in a pathogen were detected on a microarray slide spotted with 4,071 open reading frames (ORFs) of W3110, a commonly used wild-type K-12 strain. For 22 strains subjected to the CGH microarray analyses 1,424 ORFs were found to be absent in at least one strain. The common backbone of the E. coli genome was estimated to contain about 2,800 ORFs. The mosaic distribution of absent regions indicated that the genomes of pathogenic strains were highly diversified because of insertions and deletions. Prophages, cell envelope genes, transporter genes, and regulator genes in the K-12 genome often were not present in pathogens. The gene contents of the strains tested were recognized as a matrix for a neighbor-joining analysis. The phylogenic tree obtained was consistent with the results of previous studies. However, unique relationships between enteroinvasive strains and Shigella, uropathogenic, and some enteropathogenic strains were suggested by the results of this study. The data demonstrated that the CGH microarray technique is useful not only for genomic comparisons but also for phylogenic analysis of E. coli at the strain level.
• An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and lambda Red.
  Satoru Fukiya; Hiroshi Mizoguchi; Hideo Mori
  FEMS microbiology letters, 234, 2, 325, 31, 2, 15 May 2004, [Peer-reviewed], [Lead author], [International Magazine]
  English, We have established an improved large deletion method in Escherichia coli genome using a combination of two different recombination systems, lambda Red and Cre/loxP. The loxP site could be rapidly and efficiently integrated in the genome by lambda Red and large deletions of both 117- and 165-kbp regions could be generated in 100% efficiency by Cre/loxP. Comparative genomic hybridization microarray experiments of deletion strains indicated that deletions were generated only in expected regions of the genome. These results have demonstrated that the method is useful for genome engineering in E. coli.
• Occan, a novel transposon in the Fot1 family, is ubiquitously found in several Magnaporthe grisea isolates.
  Hideki Kito; Yosuke Takahashi; Junko Sato; Satoru Fukiya; Teruo Sone; Fusao Tomita
  Current genetics, 42, 6, 322, 31, 6, Mar. 2003, [Peer-reviewed], [International Magazine]
  English, Scientific journal, We investigated a DNA fragment and its flanking region deleted in the spontaneous Pi-a virulent mutant of Magnaporthe grisea Ina168. A new transposon-like sequence was identified from a region adjacent to the deleted fragment and was named Occan. Occan contained a 2,259-bp ORF interrupted by one 63-bp intron and had both a TA dinucleotide and 77 bp of perfect inverted repeats at both termini, without direct repeats. These features indicated that Occan is a member of the Fot1 family. RT-PCR analysis confirmed the expression of the putative transposase and the presence of an intron. Southern analysis of pulse-field gel electrophoresis-separated chromosomes indicated that Occan was dispersed in all chromosomes of the rice pathogen, Ina168. Copy numbers of Occan were also preserved in a host-specific manner amongst M. grisea isolates. In particular, rice pathogens contained a large number of the element inserted into their genome. Phylogenetic analysis with other known members of the Fot1 family revealed that Occan was dissimilar to any other known elements and it is thus proposed that Occan be separated to a new subfamily.
• Identification of a putative vacuolar serine protease gene in the rice blast fungus, Magnaporthe grisea.
  Satoru Fukiya; Takao Kuge; Tomomi Tanishima; Teruo Sone; Takashi Kamakura; Isamu Yamaguchi; Fusao Tomita
  Bioscience, biotechnology, and biochemistry, 66, 3, 663, 6, 3, Mar. 2002, [Peer-reviewed], [Lead author], [International Magazine]
  English, Scientific journal, We identified and cloned a gene designated SPM1, encoding a serine protease from the rice blast fungus Magnaporthe grisea. SPM1 is a single-copy gene, encoding a subtilisin-like serine protease with 536 amino acids. Analyses of the deduced amino acid sequence of SPM1 suggested that SPM1 would be localized in a vacuole, an important organelle in pathogenicity.
• Establishment of a new cross of the rice blast fungus derived from Japanese differential strain Ina168 and hermaphroditic rice pathogen Guy11
  S Fukiya; M Kodama; H Kito; T Sone; F Tomita
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 65, 7, 1464, 1473, Jul. 2001, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Molecular structure of rDNA repeat unit in Magnaporthe grisea
  T Sone; S Fukiya; M Kodama; F Tomita
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 64, 8, 1733, 1736, Aug. 2000, [Peer-reviewed]
  English, Scientific journal

• ビフィズス菌の腸内生存におけるアミノ酸生合成遺伝子の重要性の解明
  山上 暢仁; 山口 颯人; 前田 智也; 吹谷 智, 日本乳酸菌学会誌, 36, 3, 129, 129, Oct. 2025
  日本乳酸菌学会, Japanese
• Improved fermentative GABA production from glucose by inactivation of respiratory chain components NDH-I and Cytbo₃ in Escherichia coli
  若原洋輝; 溝越拓哉; 吹谷智; 横田篤; 前田智也, 日本農芸化学会大会講演要旨集(Web), 2024, 2024
• Gene function analysis to elucidate the metabolic mechanism of 1-kestose in Bifidobacterium longum subsp. longum.
  樋野優子; 難波有希; 宮下聖也; 前田智也; 吹谷智, 日本乳酸菌学会誌, 35, 2, 142, 142, 2024
  日本乳酸菌学会, Japanese
• Adaptive laboratory evolution of Escherichia coli mutants lacking the enzymes involved in the maintenance of energy and redox balance
  山上晃汰郎; 小谷葉月; 吹谷智; 古澤力; 古澤力; 前田智也; 前田智也, 日本生物工学会大会講演要旨集, 76th, 93, 93, 2024
  (公社)日本生物工学会, Japanese
• Adaptive laboratory evolution of Corynebacterium glutamicum lacking the enzymes involved in the oxidation of NADH
  中堀祥悟; 小谷葉月; 吹谷智; 古澤力; 古澤力; BOTT Michael; 前田智也; 前田智也, 日本生物工学会大会講演要旨集, 76th, 93, 93, 2024
  (公社)日本生物工学会, Japanese
• Laboratory evolution of Escherichia coli under antibiotic treatments when embedded in a synthetic gut bacterial community
  永野有佳理; 小谷葉月; 吹谷智; 古澤力; 古澤力; 前田智也; 前田智也, 日本農芸化学会大会講演要旨集(Web), 2024, 2024
• Analysis of Microbial Interactions through Extensive Serial Culturing of Human Gut Microbiota
  前田智也; 前田智也; 吹谷智; 古澤力; 古澤力, 日本農芸化学会大会講演要旨集(Web), 2024, 2024
• Laboratory evolution of Escherichia coli under two-drug combinations with sulfamethoxazole/trimethoprim
  後藤琉; 小谷葉月; 吹谷智; 古澤力; 古澤力; 前田智也; 前田智也, 日本農芸化学会大会講演要旨集(Web), 2024, 2024
• Elucidation of the abundance of deoxycholic acid-producing bacteria in human feces by quantitative PCR analysis
  原田恕; 前田智也; 後藤恭宏; 林哲也; 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2024, 2024
• Study on 8-Prenylnaringenin production (1) Screening of novel-producing bacteria from human feces and efficient production by co-culture
  丹野麻由; 植村圭祐; 大谷彬; 中島賢則; 三橋和也; 山本浩明; 前田智也; 横田篤; 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2024, 2024
• Study on 8-Prenylnaringenin production (2) Screening of bacteria to enhance demethylation and its application
  大谷彬; 中島賢則; 三橋和也; 丹野麻由; 前田智也; 横田篤; 吹谷智; 山本浩明, 日本農芸化学会大会講演要旨集(Web), 2024, 2024
• ビフィズス菌を選択的に増殖させるガラクトシル-β-1,4-ラムノースを用いた偽膜性腸炎原因菌の生育抑制効果
  平野里佳; 平野里佳; 阪中幹祥; 吉見一人; 吉見一人; 山内祐子; 山内祐子; 後藤愛那; 片山高嶺; 片山高嶺; 飯田宗穂; 加藤完; 大野博司; 吹谷智; 横田篤; 西本完; 北岡本光; 北岡本光; 中井博之; 栗原新; 栗原新, 日本実験動物学会総会講演要旨集(Web), 70th, 2023
• Elucidation of the metabolic mechanism of 1-Kestose in Bifidobacterium longum subsp. longum
  難波有希; 倉持碧海; 山口颯人; 吉田佳鼓; 後藤恭宏; 門田吉弘; 栃尾巧; 小椋義俊; 林哲也; 前田智也; 横田篤; 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2023, 2023
• Elucidation of the mechanism of ursocholic acid metabolic relay by the intestinal bacteria
  月舘一瑳; 北川更; 前田智也; 吹谷智; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2023, 2023
• Discussion about survival strategies of bifidobacteria in the gastrointestinal tract of conventional mice based on the comprehensively-identified bifidobacterial genes using insertion-sequencing
  山口颯人; 倉持碧海; 後藤恭宏; 小椋義俊; 前田智也; 林哲也; 横田篤; 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2023, 2023
• Expression in Escherichia coli and functional analysis of cystathionine-metabolizing enzymes of bifodobacteria
  和田大; 宮森那知; 吹谷智; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2023, 2023
• 定量PCR解析によるヒト糞便中のデオキシコール酸生成菌の存在量の解明
  原田恕; 前田智也; 後藤恭宏; 林哲也; 吹谷智, 日本農芸化学会北海道支部学術講演会講演要旨集(Web), 2023, 2023
• 実験室進化による大腸菌のエネルギー欠乏への潜在的な適応能力の解明
  山上晃汰郎; 小谷葉月; 吹谷智; 古澤力; 古澤力; 前田智也; 前田智也, 日本農芸化学会北海道支部学術講演会講演要旨集(Web), 2023, 2023
• Establishment of a selective recovery system for bifidobacteria from the gut microbiota
  宮下聖也; 吉田佳鼓; 嶋田みな; 前田智也; 横田篤; 吹谷智, 日本生物工学会大会講演要旨集, 75th, 201, 201, 2023
  (公社)日本生物工学会, Japanese
• 高脂肪食摂取ラット盲腸で増加した遊離脂肪酸種が腸内細菌に与える影響の評価
  川上志野; 川上憲太郎; GOWDA Siddabasave Gowda B.; CHEN Zhen; 吹谷智; LIANG Chongsheng; HAI Han; 石塚敏; 小椋義俊; 後藤恭宏; 前田智也; 林哲也; 千葉仁志; 惠淑萍; 横田篤, 日本農芸化学会北海道支部学術講演会講演要旨集(Web), 2023, 2023
• 腸内でビフィズス菌優勢な菌叢が形成・維持される機構の解明とその意義 Insertion Sequencing法によるビフィズス菌の腸内生存に寄与する遺伝子の同定
  吹谷 智, 日本細菌学雑誌, 77, 1, 43, 43, Feb. 2022
  日本細菌学会, Japanese
• Intestinal secondary bile acid production and its induction of the gut microbiota alteration and disease development
  吹谷智; 横田篤; 石塚敏, 機能性食品と薬理栄養, 15, 4, 229, 234, Feb. 2022, [Invited], [Lead author, Corresponding author]
  (株)インフォノーツパブリッシング, Japanese, Introduction other
• Improved fermentative GABA production from glucose by inactivation of respiratory chain components in Escherichia coli.
  若原洋輝; 溝越拓哉; 前田智也; 吹谷智; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2022, 2022
• Identification of bifidobacterial genes contributing to intestinal survival by insertion sequencing
  吹谷智, 日本細菌学雑誌(Web), 77, 1, 2022
• Establishment of a selective recovery system for bifidobacteria from the gut microbiota.
  宮下聖也; 吉田佳鼓; 嶋田みな; 前田智也; 横田篤; 吹谷智, 日本乳酸菌学会誌, 33, 2, 121, 121, 2022
  日本乳酸菌学会, Japanese
• Evaluation of the contribution of the bifidobacterial genes involved in amino acid metabolism identified by Insertion Sequencing analysis to colonization and survival in the conventional mouse intestine
  井上太貴; 前田智也; 横田篤; 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2022, 2022
• 大腸菌呼吸鎖変異株ΔNDH-IΔCytbo₃を用いたグルコースからのGABAの高生産
  若原洋輝; 溝越拓哉; 前田智也; 吹谷智; 横田篤, 日本農芸化学会東北支部大会プログラム・講演要旨集, 2022, 2022
• Identification of enzyme genes involved in Cysteine/Methionine metabolism in Bifidobacterium longum 105-A
  宮森那知; 松本菜々恵; 吹谷智; 横田篤; 和田大, 日本農芸化学会大会講演要旨集(Web), 2022, 2022
• 定量PCR解析によるヒト糞便中のデオキシコール酸生成菌の存在量の解明
  原田恕; 前田智也; 横田篤; 吹谷智, 日本農芸化学会東北支部大会プログラム・講演要旨集, 2022, 2022
• 常在腸内細菌叢からのビフィズス菌の選択的回収系の確立
  宮下聖也; 吉田佳鼓; 嶋田みな; 前田智也; 横田篤; 吹谷智, 日本農芸化学会東北支部大会プログラム・講演要旨集, 2022, 2022
• Establishment of a selective recovery system for bifidobacteria in the gut microbiota
  吉田佳鼓; 嶋田みな; 宮下聖也; 前田智也; 横田篤; 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2022, 2022
• Identification of bifidobacterial genes that contribute to survival and colonization in the gastrointestinal tract of conventional mice using insertion-sequencing
  山口颯人; 倉持碧海; 後藤恭宏; 小椋義俊; 前田智也; 林哲也; 横田篤; 吹谷智, 日本生物工学会大会講演要旨集, 74th, 158, 158, 2022
  (公社)日本生物工学会, Japanese
• Elucidation of the effects of the fatty acid profile changes on the gut microbiota composition in the cecum of rats fed a high-fat diet: validation of a fatty-acid hypothesis
  川上憲太郎; GOWDA Siddabasave Gowda B.; CHEN Zhen; 吹谷智; LIANG Chongsheng; HAI Han; 石塚敏; 小椋義俊; 後藤恭宏; 前田智也; 林哲也; 千葉仁志; 惠淑萍; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2022, 2022
• Elucidation of the metabolic mechanism of 1-Kestose in Bifidobacterium longum subsp. longum
  難波有希; 倉持碧海; 山口颯人; 吉田佳鼓; 後藤恭宏; 門田吉弘; 栃尾巧; 小椋義俊; 林哲也; 前田智也; 横田篤; 吹谷智, 日本乳酸菌学会誌, 33, 2, 120, 120, 2022
  日本乳酸菌学会, Japanese
• Effect of deleting genes encoding glutamate dehydrogenase or glutamate synthase on unusual glutamate production in Escherichia coli mutant defective in respiratory chain enzymes NDH-I and Cytbo₃
  今壮太; 前田智也; 吹谷智; 和田大; 横田篤, 日本生物工学会大会講演要旨集, 73rd, 2021
• ビフィズス菌が有するフコシルラクトース輸送体の多様性
  OJIMA Miriam N.; 浅尾有哉; 中島有登; 加藤紀彦; 北岡本光; 後藤愛那; 廣瀬潤子; 浦島匡; 吹谷智; 横田篤; HACHEM Maher Abou; 阪中幹祥; 片山高嶺, ミルクサイエンス(Web), 70, 2, 2021
• Insertion Sequencing解析により網羅的に同定されたビフィズス菌遺伝子の通常飼育マウス腸内における定着と生存への寄与の評価
  井上太貴; 前田智也; 横田篤; 吹谷智, 日本農芸化学会北海道支部学術講演会講演要旨集(Web), 2021, 2021
• 高脂肪食摂取ラットにおける盲腸内脂肪酸プロファイルの変化が腸内細菌叢構成に与える影響
  川上憲太郎; GOWDA B. Gowda Siddabasave; CHEN Zhen; 吹谷智; LIANG Chongsheng; HAI Han; 石塚敏; 小椋義俊; 後藤恭宏; 前田智也; 林哲也; 千葉仁志; 惠淑萍; 横田篤, 日本農芸化学会北海道支部学術講演会講演要旨集(Web), 2021, 2021
• Elucidation of the metabolic mechanism of 1-Kestose in Bifidobacterium longum subsp. longum
  難波有希; 山口颯人; 吉田佳鼓; 後藤恭宏; 門田吉弘; 栃尾巧; 小椋義俊; 林哲也; 横田篤; 吹谷智, 日本生物工学会大会講演要旨集, 73rd, 230, 230, 2021
  (公社)日本生物工学会, Japanese
• Mucin O-glycan assimilation pathway in Bifidobacterium
  CHOI Moonhak; 後藤愛那; 阪中幹祥; 加藤紀彦; 西本完; 杉山友太; 栗原新; 吹谷智; 横田篤; 北岡本光; 片山高嶺, 日本農芸化学会大会講演要旨集(Web), 2021, 2021
• Comparison of contributions of pyruvate carboxylase and phosphoenolpyruvate carboxylase reactions on lysine production by Corynebacterium glutamicum
  新森彰信; GUO Zhen; 吹谷智; 和田大; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2021, 2021
• Elucidation of the mechanism of enhanced lysine production by mutation in citrate synthase gene leading to its reduced activity in Corynebacterium glutamicum
  大塚萌音; GUO Zhen; 佐藤桃梨; 愛甲徹; 柳瀬真紀; 吹谷智; 和田大; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2021, 2021
• Identification of enzyme genes involved in reverse transsulfuration pathway in Bifidobacterium longum 105-A using gene-knockout mutants
  宮森那知; 松本菜々恵; 吹谷智; 横田篤; 和田大, 日本農芸化学会大会講演要旨集(Web), 2021, 2021
• Evaluation of the bifidobacterial genes for contribution to colonization and survival in the conventional mouse intestine as identified by Insertion-Sequencing
  井上太貴; 横田篤; 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2021, 2021
• Identification and functional verification of enzyme genes constituting reverse transsulfuration pathway in Bifidobacterium longum 105-A
  宮森那知; 松本菜々恵; 吹谷智; 横田篤; 和田大, 日本乳酸菌学会誌, 32, 2, 71, 71, 2021
  日本乳酸菌学会, Japanese
• Identification of bifidobacterial genes that contribute to survival and colonization in the gastrointestinal tract of conventional mice using insertion-sequencing.
  山口颯人; 倉持碧海; 後藤恭宏; 小椋義俊; 園山慶; 林哲也; 横田篤; 吹谷智, 日本乳酸菌学会誌, 32, 2, 70, 70, 2021
  日本乳酸菌学会, Japanese
• Bile acid as a responsible factor for high-fat diet-induced gut microbiota alterations: Proof of “bile acid hypothesis“
  横田篤; 渡辺真通; 藤田祐己; 石塚敏; 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2020, 2020
• Association between 12α-hydroxylated bile acids and hepatic steatosis
  堀将太; 阿部隆行; LEE DongGeun; 吹谷智; 横田篤; 麻生菜央; 城内文吾; 佐藤匡央; 石塚敏, 日本農芸化学会大会講演要旨集(Web), 2020, 2020
• ラフィノース摂取によるラット腸内でのデオキシコール酸生成の抑制効果の解明
  小山遥; 佐竹実菜子; 前川顕汰; 吹谷智; 小椋義俊; 林哲也; 石塚敏; 横田篤, 日本農芸化学会北海道支部講演会講演要旨(Web), 2019, 2019
• ビフィズス菌のトランスポゾン変異株を用いたα-グルコシダーゼ遺伝子の機能解明
  阪中幹祥; 阪中幹祥; 阪中幹祥; 中川路伸吾; 中島森; 阿部光紗; 佐分利亘; 森春英; 横田篤; 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2019, 2019
• 高脂肪食中に含まれる脂肪酸がラット盲腸内細菌叢構成に与える影響の解明:脂肪酸仮説の検証
  阿部隆行; 渡辺真通; 藤田祐己; 小椋義俊; 吹谷智; 石塚敏; 林哲也; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2019, 2019
• 高脂肪食またはコール酸摂取マウス腸内における胆汁酸組成および細菌叢構成の動態解析による胆汁酸仮説の検証
  坂本佳奈子; 相磯知里; 松浦史佳; 吹谷智; 園山慶; 小椋義俊; 林哲也; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2019, 2019
• 胆汁酸変換菌の共培養によるウルソコール酸からデオキシコール酸への変換機構の検証
  北川更; TAWTHEP Saryinya; 吹谷智; 小椋義俊; 林哲也; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2019, 2019
• Bifidobacterium animalis subsp.lactisが有するストレス耐性遺伝子の探索
  齋藤史; 磯奏子; 尾淵駿; 吹谷智; 横田篤; 山本裕司; 向井孝夫, 日本乳酸菌学会誌, 30, 2, 113, 113, 2019
  日本乳酸菌学会, Japanese
• 機能性糖質によるラット盲腸内細菌叢の改変に基づいたデオキシコール酸生成菌と相互作用する腸内細菌種の同定
  吹谷智; 佐竹実菜子; 佐藤昌直; 石塚敏; 小椋義俊; 林哲也; 横田篤, 日本乳酸菌学会誌, 30, 2, 103, 103, 2019
  日本乳酸菌学会, Japanese
• 胆汁酸変換菌の相互作用によるウルソコール酸からデオキシコール酸への新たな変換経路の同定とその変換機構の解明
  北川更; TAWTHEP Sarinya; 吹谷智; 小椋義俊; 林哲也; 横田篤, 日本乳酸菌学会誌, 30, 2, 104, 104, 2019
  日本乳酸菌学会, Japanese
• エクオール産生におよぼす胆汁酸の影響
  吉岡寛子; 渡辺真通; 難波文男; 鈴木利雄; 吹谷智; 横田篤; 戸田登志也, 日本農芸化学会大会講演要旨集(Web), 2019, 2019
• イソフラボン代謝におよぼす胆汁酸の影響
  吉岡寛子; 渡辺真通; 難波文男; 鈴木利雄; 吹谷智; 横田篤; 戸田登志也, 日本栄養・食糧学会大会講演要旨集, 73rd, 242, 242, 2019
  (公社)日本栄養・食糧学会, Japanese
• ビフィズス菌の含硫アミノ酸代謝に関与するC-Sリアーゼの解析
  黒瀧空; 松本菜々恵; 吹谷智; 横田篤; 和田大, 日本生物工学会大会講演要旨集, 71st, 276, 276, 2019
  (公社)日本生物工学会, Japanese
• 慢性的な高脂肪食摂取によって腸肝局所で増加する12α水酸化胆汁酸はインスリン感受性を向上させる一方で肝臓脂質蓄積を亢進する
  堀将太; 吉次玲香; 菊池慧大; 阿部隆行; 原博; 吹谷智; 横田篤; 石塚敏, 日本栄養・食糧学会大会講演要旨集, 73rd, 245, 245, 2019
  (公社)日本栄養・食糧学会, Japanese
• Evolutionary traits found in fucosyllactose transporters support symbiosis between bifidobacteria and breast-fed infants
  阪中幹祥; 阪中幹祥; 栗原新; 栗原新; 片山高嶺; 片山高嶺; HANSEN Morten Ejby; 後藤愛那; 加藤紀彦; 吉田圭佑; 小田巻俊孝; 杉山友太; 廣瀬潤子; 浦島匡; 清水(肖)金忠; 北岡本光; 吹谷智; 横田篤; LEGGIO Leila Lo; HACHEM Maher Abou, Institute for Fermentation, Osaka. Research Communications, 33, 2019
• Bifidobacterium bifidumの細胞外シアリダーゼの腸粘液との相互作用と糖の資化における役割
  西山啓太; 長井暁; 吹谷智; 横田篤; 山本裕司; 向井孝夫; 岡田信彦, 腸内細菌学雑誌, 32, 2, 102, 102, Apr. 2018
  (公財)腸内細菌学会, Japanese
• ビフィズス菌におけるラクト-N-テトラオース輸送体の同定およびビフィズスフローラ形成への関与
  阪中 幹祥; 砂川 直輝; 佐藤 真与; 吉田 圭佑; 石田 卓也; 後藤 愛那; 加藤 紀彦; 谷内 寛之; 密山 恵梨; 加藤 久美子; 廣瀬 潤子; 阿部 紘一; 前田 信悟; 杉山 友太; 吹谷 智; 横田 篤; 栗原 新; 荒川 孝俊; 小田巻 俊孝; 清水 金忠; 五十嵐 圭日子; 伏信 進矢; 片山 高嶺, 腸内細菌学雑誌, 32, 2, 101, 101, Apr. 2018
  (公財)日本ビフィズス菌センター, Japanese
• 大腸菌のNADH脱水素酵素Iとシトクロムbo₃オキシダーゼ両欠損株の糖代謝に与えるグルタミン酸脱炭酸/膜輸送系の影響
  和田浩; 瀬島祐大; 吹谷智; 和田大; 横田篤, 日本農芸化学会北海道支部講演会講演要旨, 2018, 2018
• トランスポゾン変異株集団を用いたビフィズス菌の腸内生存戦略の解明手法の確立
  中島森; 久保勇貴; 中川路伸吾; 阪中幹祥; 小椋義俊; 林哲也; 横田篤; 吹谷智, 日本ゲノム微生物学会年会要旨集, 12th, 2018
• ラットへのコール酸および難消化性オリゴ糖の同時投与による腸内細菌叢改変に基づいたデオキシコール酸生成菌と相互作用する腸内細菌種の探索
  佐竹実菜子; 吹谷智; 佐藤昌直; 石塚敏; 小椋義俊; 林哲也; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2018, 2018
• 高脂肪食摂取ラットにおける盲腸内細菌叢変動機構の解析:「胆汁酸仮説」の証明に向けて
  横田篤; 渡辺真通; 藤田祐己; 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2018, 2018
• 乳酸桿菌の対数期に膜小胞上に局在するリポテイコ酸
  白石宗; 横田伸一; 佐藤耶舞羽; 伊藤利章; 吹谷智; 山本聡; 佐藤豊孝; 横田篤, 日本乳酸菌学会誌, 29, 2, 116, 116, 2018
  日本乳酸菌学会, Japanese
• ビフィズス菌の腸内生存・定着機構の解明に向けたINSeq法の確立とその評価
  中島森; 久保勇貴; 中川路伸吾; 阪中幹祥; 小椋義俊; 吉本真; 吉田圭佑; 清水(肖)金忠; 小田巻俊孝; 林哲也; 横田篤; 吹谷智, 日本乳酸菌学会誌, 29, 2, 109, 109, 2018
  日本乳酸菌学会, Japanese
• ビフィズス菌における低温生残性向上機構の解析
  齋藤史; 磯奏子; 小泉亮子; 原翔; 吹谷智; 横田篤; 山本裕司; 向井孝夫, 日本乳酸菌学会誌, 29, 2, 108, 108, 2018
  日本乳酸菌学会, Japanese
• ヒト由来ビフィズス菌Bifidobacterium longum105-Aが通常飼育マウス腸内に定着可能な飼育試験系の確立
  嶋田みな; 川瀬陽平; 園山慶; 小椋義俊; 林哲也; 横田篤; 吹谷智, 日本乳酸菌学会誌, 29, 2, 110, 110, 2018
  日本乳酸菌学会, Japanese
• 大腸菌のNADH脱水素酵素Iとシトクロムbo₃オキシダーゼ両欠損株におけるNDH-II過剰発現が中枢代謝に与える影響
  濱田和宏; 瀬島祐大; 吹谷智; 和田大; 横田篤, 日本生物工学会大会講演要旨集, 70th, 2018
• マウス腸内への定着に寄与するビフィズス菌遺伝子の網羅的同定に向けたINSeq法の確立
  久保勇貴; 中島森; 中川路伸吾; 阪中幹祥; 小椋義俊; 吉本真; 吉田圭佑; XIAO Jin-Zhong; 小田巻俊孝; 林哲也; 横田篤; 吹谷智, 日本生物工学会大会講演要旨集, 70th, 2018
• A unique species-specific structure of lipoteichoic acid common to Lactobacillus gasseri
  白石宗; 久富亮佑; 佐藤耶舞羽; 森田直樹; 吹谷智; 佐藤豊孝; 横田篤; 横田伸一, エンドトキシン・自然免疫研究(Web), 21, 35, 37, 2018
  (一社)日本エンドトキシン・自然免疫研究会, Japanese
• ABCトランスポーターを介したビフィズス菌の糖質取込みを包括的に制御するATP加水分解酵素MsiK
  阪中幹祥; 前田信悟; 後藤愛那; 村上隆太; 加藤紀彦; 杉山友太; 谷内寛之; 栗原新; 玉置尚徳; 吹谷智; 横田篤; 片山高嶺; 片山高嶺, 日本乳酸菌学会誌, 29, 2, 107, 107, 2018
  日本乳酸菌学会, Japanese
• ビフィズス菌由来ATPase MsiKはABCトランスポーターを介した糖質取込みをグローバルに制御する
  前田信悟; 阪中幹祥; 後藤愛那; 村上隆太; 加藤紀彦; 杉山友太; 谷内寛之; 栗原新; 玉置尚徳; 吹谷智; 横田篤; 片山高嶺; 片山高嶺, 日本農芸化学会大会講演要旨集(Web), 2018, 2018
• セミナー室 腸内相互作用の理解に基づいた健康の増進・疾患の予防(6)腸内細菌叢の機能理解に向けて : ビフィズス菌における遺伝子操作系の開発
  吹谷 智; 横田 篤, 化学と生物 : 日本農芸化学会会誌 : 生命・食・環境, 55, 9, 637, 643, Sep. 2017
  日本農芸化学会 ; 1962-, Japanese
• 大腸菌のNADH脱水素酵素Iとシトクロムbo₃オキシダーゼ両欠損株における異常な糖代謝の解析
  瀬島祐大; 紀平千枝; 吹谷智; 和田大; 横田篤, 日本生物工学会大会講演要旨集, 69th, 220, 220, 08 Aug. 2017
  (公社)日本生物工学会, Japanese
• ビフィズス菌におけるgalacto-N-biose/lacto-N-biose Iトランスポーターの多様性解析 母乳オリゴ糖lacto-N-tetraoseの取込みに寄与するアミノ酸配列の発見
  阪中 幹祥; 石田 卓也; 佐藤 真与; 谷内 寛之; 阿部 紘一; 前田 信悟; 吹谷 智; 横田 篤; 栗原 新; 荒川 孝俊; 五十嵐 圭日子; 伏信 進矢; 片山 高嶺, 日本乳酸菌学会誌, 28, 2, 127, 127, Jun. 2017
  日本乳酸菌学会, Japanese
• Bifidobacterium longum105‐Aのメチオニン利用に関与する遺伝子の発現解析および遺伝子破壊による検証
  和田大; 松本菜々恵; 小松一喜; 阪中幹祥; 松尾実紀; 吹谷智; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2017, ROMBUNNO.4C24a04 (WEB ONLY), 05 Mar. 2017
  Japanese
• Bifidobacterium bifidumにおけるシアリダーゼは宿主との相互作用を促進し糖の資化をサポートする
  西山啓太; 山本裕司; 杉山真言; 篠原有香; 浦島匡; 吹谷智; 横田篤; 岡田信彦; 向井孝夫, 日本農芸化学会大会講演要旨集(Web), 2017, ROMBUNNO.3C18p05 (WEB ONLY), 05 Mar. 2017
  Japanese
• Lactobacillus gasseri種に共通するリポテイコ酸の特異的構造
  久富 亮佑; 白石 宗; 佐藤 耶舞羽; 森田 直樹; 吹谷 智; 佐藤 豊孝; 横田 篤; 横田 伸一, 日本細菌学雑誌, 72, 1, 86, 86, Feb. 2017
  日本細菌学会, Japanese
• 腸内細菌の代謝リレーによるデオキシコール酸生成に関与する7α-ヒドロキシステロイドデヒドロゲナーゼの反応特性の解析
  柴野可菜; TAWTHEP Sarinya; 鳥居剛史; 小椋義俊; 林哲也; 吹谷智; 和田大; 横田篤, 日本農芸化学会北海道支部講演会講演要旨, 2017, 2017
• Bifidobacterium animalis subsp.lactisにおけるストレス耐性遺伝子の探索
  磯奏子; 齋藤史; 原翔; 小泉亮子; 大塚雅也; 吹谷智; 横田篤; 山本裕司; 向井孝夫, ミルクサイエンス, 66, 2, 173, 173, 2017
  日本酪農科学会, Japanese
• Lactobacillus gasseri種に共通する種特異的なリポテイコ酸の化学構造
  久富亮佑; 白石宗; 佐藤耶舞羽; 森田直樹; 吹谷智; 佐藤豊孝; 横田篤; 横田伸一, 日本乳酸菌学会誌, 28, 2, 123, 123, 2017
  日本乳酸菌学会, Japanese
• 高脂肪食摂取によるマウス腸内の胆汁酸量の増加が腸内細菌叢構成に与える影響
  相磯知里; 松浦史佳; 吹谷智; 渡辺真通; 園山慶; 小椋義俊; 林哲也; 横田篤, 日本乳酸菌学会誌, 28, 2, 137, 137, 2017
  日本乳酸菌学会, Japanese
• コール酸・ケノデオキシコール酸・デオキシコール酸各投与マウスにおける盲腸内胆汁酸組成および菌叢構成の変化
  松浦史佳; 相磯知里; 吹谷智; 渡辺真通; 園山慶; 小椋義俊; 林哲也; 横田篤, 日本乳酸菌学会誌, 28, 2, 138, 138, 2017
  日本乳酸菌学会, Japanese
• 消化管における生存と定着に寄与するビフィズス菌遺伝子の探索系の構築
  中川路伸吾; 中島森; 阪中幹祥; 小椋義俊; 林哲也; 横田篤; 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2017, 2017
• 二種の腸内細菌の共培養によって明らかになったデオキシコール酸生成の活性化と新たな生成経路
  TAWTHEP Sarinya; 鳥居剛史; 山崎真侑; 吹谷智; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2017, 2017
• 消化管における生存と定着に寄与するビフィズス菌遺伝子の網羅的同定に向けたINSeq法の確立
  中島森; 中川路伸吾; 阪中幹祥; 小椋義俊; 林哲也; 横田篤; 吹谷智, 日本生物工学会大会講演要旨集, 69th, 98, 98, 2017
  (公社)日本生物工学会, Japanese
• Lactobacillus gasseri種に共通するリポテイコ酸の特異的構造
  久富亮佑; 白石宗; 佐藤耶舞羽; 森田直樹; 吹谷智; 佐藤豊孝; 横田篤; 横田伸一, 日本細菌学雑誌(Web), 72, 1, 2017
• 構造情報の蓄積から見えてきたリポテイコ酸の細菌種による構造多様性.
  白石宗; 横田伸一; 吹谷智; 横田篤, 日本乳酸菌学会誌, 28, 3, 177, 177, 2017, [Invited]
  日本乳酸菌学会, Japanese
• 二種の腸内細菌の代謝リレーによる新たなデオキシコール酸生成経路の発見
  Tawthep Sarinya; 鳥居 剛史; 吹谷 智; 横田 篤, 日本乳酸菌学会誌, 27, 2, 133, 133, Jun. 2016
  日本乳酸菌学会, English
• Bifidobacterium longum105‐Aのメチオニン利用性に関与するS‐アデノシルメチオニン回路の解析
  松本菜々恵; 小松一喜; 阪中幹祥; 鈴木梓; 吹谷智; 横田篤; 和田大, 日本農芸化学会大会講演要旨集(Web), 2016, 2F138 (WEB ONLY), 05 Mar. 2016
  Japanese
• 呼吸鎖改変大腸菌ΔNDH‐IΔCyt boの酸素制限による糖代謝の変化
  杉本諒; 加藤竜一; 荒井博紀; 紀平知枝; 吹谷智; 和田大; 片岡尚也; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2016, 3F144 (WEB ONLY), 05 Mar. 2016
  Japanese
• ビフィズス菌の2つの遺伝子変異導入系の確立:標的遺伝子への欠失変異導入系とトランスポゾン変異導入系
  吹谷智, 日本農芸化学会北海道支部講演会講演要旨, 2016, 2016
• 高脂肪食摂取がマウスの胆汁酸組成と腸内細菌叢構成に与える影響
  相磯知里; 松浦史佳; 吹谷智; 渡辺真通; 園山慶; 小椋義俊; 林哲也; 横田篤, 日本農芸化学会北海道支部講演会講演要旨, 2016, 2016
• 胆汁酸摂取マウスの腸内における胆汁酸組成および菌叢の動態解析
  松浦史佳; 相磯知里; 吹谷智; 渡辺真通; 園山慶; 小椋義俊; 林哲也; 横田篤, 日本農芸化学会北海道支部講演会講演要旨, 2016, 2016
• 高脂肪食摂取ラットの盲腸内菌叢の変化における胆汁酸の寄与:「胆汁酸仮説」の証明にむけて
  渡辺真通; 萩尾真人; 李慈英; 小椋義俊; 林哲也; 清水英寿; 石塚敏; 吹谷智; 横田篤, 腸内細菌学雑誌, 30, 2, 91, 91, 2016
  (公財)腸内細菌学会, Japanese
• 高脂肪食摂取ラットの盲腸内菌叢の変化における腸内細菌の胆汁酸耐性の寄与
  渡辺真通; 萩尾真人; LEE Ja-Young; 小椋義俊; 林哲也; 清水英寿; 石塚敏; 吹谷智; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2016, 2016
• 対数期におけるLactobacillus gasseri JCM1131^Tのリポテイコ酸の局在
  白石宗; 横田伸一; 佐藤耶舞羽; 伊藤利章; 吹谷智; 山本聡; 小笠原徳子; 佐藤豊孝; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2016, 2016
• 腸内生存に寄与するビフィズス菌遺伝子の探索系の構築
  中川路伸吾; 阪中幹祥; 横田篤; 吹谷智, 日本農芸化学会大会講演要旨集(Web), 2016, 2016
• R-IVET法によるマウス消化管特異的に発現するビフィズス菌遺伝子の同定
  石神夏実; 河口礼佳; 平等清夏; 阪中幹祥; 横田篤; 吹谷智, 日本生物工学会大会講演要旨集, 68th, 223, 223, 2016
  (公社)日本生物工学会, Japanese
• マウス消化管内で特異的に発現するビフィズス菌遺伝子の同定法の確立
  河口礼佳; 平等清夏; 石神夏実; 阪中幹祥; 横田篤; 吹谷智, 日本ゲノム微生物学会年会要旨集, 10th, 2016
• トランスポゾン変異導入系を用いたビフィズス菌の腸内生存に寄与する遺伝子の探索系の構築
  中川路伸吾; 阪中幹祥; 横田篤; 吹谷智, 日本ゲノム微生物学会年会要旨集, 10th, 2016
• 食品タンパク質由来腸内細菌代謝産物スカトールが糞中胆汁酸組成および肝臓に与える影響
  清水英寿; 清水英寿; 田中愛建; 野勢琢馬; 吹谷智; 横田篤; 石塚敏, 日本栄養・食糧学会中国・四国支部大会講演要旨集, 49th, 2016
• Lactobacillus gasseri JCM 1131Tの対数期におけるリポテイコ酸の局在：Membrane vesicle表層に露出されるリポテイコ酸
  白石宗; 佐藤耶舞羽; 横田伸一; 伊藤利章; 吹谷智; 横田篤, エンドトキシン・自然免疫研究19－新たなる基礎と臨床の架け橋－, 19, 15, 17, 2016
  (一社)日本エンドトキシン・自然免疫研究会, Japanese, Introduction commerce magazine
• コール酸誘導性脂肪肝発症に伴う脂質組成と遺伝子発現
  花井 健人; 辻 美咲; 吉次 玲香; 菊地 慧大; 野勢 琢馬; 多田 幸司; 清水 英寿; 萩尾 真人; 城内 文吾; 佐藤 匡央; 吹谷 智; 横田 篤; 原 博; 石塚 敏, 日本生化学会大会・日本分子生物学会年会合同大会講演要旨集, 88回・38回, [1P1197], [1P1197], Dec. 2015
  (公社)日本生化学会, Japanese
• Bifidobacterium longum 105‐Aのメチオニン利用性に関与するC‐Sリアーゼの解析
  松本菜々恵; 鈴木梓; 小松一喜; 吹谷智; 横田篤; 和田大, 日本農芸化学会北海道支部講演会講演要旨, 2015, 17, 08 Aug. 2015
  Japanese
• コール酸摂取はラット腸内細菌叢の組成を変化させ脂肪肝を誘導する
  李 慈英; 清水 英寿; 萩尾 真人; 吹谷 智; 吉次 玲香; 菊池 慧大; 馬場 菜那子; 辻 美咲; 小椋 義俊; 林 哲也; 石塚 敏; 横田 篤, 日本乳酸菌学会誌, 26, 2, 162, 162, Jun. 2015
  日本乳酸菌学会, English
• 2A-Cp01 Development of practical gene-mutagenesis systems in bifidobacteria
  Fukiya Satoru, 日本生物工学会大会講演要旨集, 67, 173, 173, 2015
  日本生物工学会, Japanese
• 高脂肪食摂取がラット盲腸内容物の胆汁酸分泌および菌叢変化に与える影響
  渡辺真通; 萩尾真人; 藤田祐己; LEE Ja-Young; 馬場菜那子; 小椋義俊; 小椋義俊; 林哲也; 林哲也; 清水英寿; 石塚敏; 吹谷智; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2015, 2015
• INSeq法を用いた腸内生存に寄与するビフィズス菌遺伝子の探索系の構築
  中川路伸吾; 阪中幹祥; 横田篤; 吹谷智, 日本乳酸菌学会誌, 26, 2, 154, 154, 2015
  日本乳酸菌学会, Japanese
• コール酸長期摂取ラットにおける腸内細菌叢変動を介した非肥満性脂肪肝の発症
  横田篤; 李慈英; 清水英寿; 萩尾真人; 吹谷智; 大岡唯祐; 小椋義俊; 林哲也; 石塚敏, 日本未病システム学会学術総会抄録集, 22nd, 67, 67, 2015
  (一社)日本未病学会, Japanese
• IS3 family挿入配列ISBlo11を用いたビフィズス菌のトランスポゾン変異導入系の確立
  阪中幹祥; 横田篤; 吹谷智, 日本ゲノム微生物学会年会要旨集, 9th, 2015
• Bifidobacterium breve JCM 1192^Tを指標菌としたラット腸内に存在する胆汁酸分子種の細胞毒性評価
  渡辺真通; 吹谷智; 横田篤, 日本農芸化学会北海道支部講演会講演要旨, 2015, 2015
• マウス腸内で特異的に発現するビフィズス菌遺伝子の探索
  河口礼佳; 石神夏実; 平等清夏; 阪中幹祥; 横田篤; 吹谷智, 日本農芸化学会北海道支部講演会講演要旨, 2015, 2015
• ビフィズス菌におけるシアリダーゼのムチン付着因子としての新たな役割
  西山啓太; 西山啓太; 篠原有香; 浦島匡; 吹谷智; 横田篤; 山本裕司; 向井孝夫, 日本乳酸菌学会誌, 26, 2, 156, 156, 2015
  日本乳酸菌学会, Japanese
• 腸内乳酸菌 Lactobacillus gasseri JCM 1131T のリポテイコ酸に認められた新奇構造
  白石宗; 横田伸一; 吹谷智; 横田篤, エンドトキシン・自然免疫研究18－自然免疫における生体防御ペプチドの多様性－, 18, 79, 81, 2015
  (一社)日本エンドトキシン・自然免疫研究会, Japanese, Introduction other
• Skatoleの経口負荷が肝Cyp7al発現と二次胆汁酸排泄の亢進を誘導する
  野勢 琢馬; 清水 英寿; 萩尾 真人; 吹谷 智; 横田 篤; 原 博; 石塚 敏, 肥満研究, 20, Suppl., 220, 220, Oct. 2014
  (一社)日本肥満学会, Japanese
• ビフィズス菌のメチオニン資化性に関与するS‐アデノシルメチオニン回路の解析
  小松一喜; 鈴木梓; 阪中幹祥; 吹谷智; 横田篤; 和田大, 日本生物工学会大会講演要旨集, 66th, 46, 46, 05 Aug. 2014
  日本生物工学会, Japanese
• 1P-115 Effects of respiratory-chain-enzyme defective mutations on central carbon metabolism in Escherichia coli
  KATOH Ryuichi; ARAI Hironori; KIHIRA Chie; FUKIYA Satoru; WADA Masaru; YOKOTA Atsushi, 日本生物工学会大会講演要旨集, 66, 46, 46, 05 Aug. 2014
  The Society for Biotechnology, Japan, Japanese
• ビフィズス菌の逆流硫黄経路の同定と活性染色法によるC‐Sリアーゼの解析
  鈴木梓; 和田大; 吹谷智; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2014, 3A15P17 (WEB ONLY), 05 Mar. 2014
  Japanese
• Lactobacillus gasseriにおける生育段階によるリポテイコ酸の局在変化
  白石宗; 横田伸一; 佐藤耶舞羽; 伊藤利章; 吹谷智; 横田篤, 日本乳酸菌学会誌, 25, 2, 2014
• 微生物のストレス応答 腸管内での胆汁酸ストレスと腸内乳酸菌の生残戦略
  白石宗; 横田伸一; 吹谷智; 横田篤, 日本乳酸菌学会誌, 25, 1, 42, 42, 2014
  日本乳酸菌学会, Japanese
• 大腸菌低活性型のビフィズス菌プロモーターの同定およびトランスポゾン変異導入系への応用
  阪中幹祥; 玉井早紀; 平山洋佑; 横田篤; 吹谷智, 日本乳酸菌学会誌, 25, 2, 2014
• in vivo特異的に発現するビフィズス菌遺伝子の同定法の確立
  河口礼佳; 平等清夏; 阪中幹祥; 横田篤; 吹谷智, 日本生物工学会大会講演要旨集, 66th, 2014
• 大腸菌およびBifidobacterium longumにおけるビフィズス菌プロモーターの機能解析
  阪中幹祥; 玉井早紀; 平山洋佑; 横田篤; 吹谷智, 日本ゲノム微生物学会年会要旨集, 8th, 2014
• Clostridium hiranonisにおけるコール酸の代謝に対するフマル酸の影響
  鳥居剛史; 本田希未; 萩尾真人; 吹谷智; 横田篤, 日本生物工学会大会講演要旨集, 66th, 46, 46, 2014
  (公社)日本生物工学会, Japanese
• コール酸経口負荷による肝脂質蓄積
  辻美咲; 吉次玲香; 菊地慧大; 野勢琢馬; 清水英寿; 李慈英; 馬場菜那子; 萩尾真人; 吹谷智; 横田篤; 原博; 石塚敏, 肥満研究, 20, Supplement, 191, 191, 2014
  (一社)日本肥満学会, Japanese
• コール酸長期摂取ラットにおける糞便中胆汁酸組成の変化と腸内細菌叢との関係
  馬場菜那子; 萩尾真人; LEE Ja-Young; 吹谷智; 小椋義俊; 小椋義俊; 清水英寿; 大岡唯祐; 林哲也; 林哲也; 石塚敏; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2014, 2014
• 胆汁酸と腸内フローラ:メタボリックシンドローム発症との関連性
  横田篤; 吹谷智; ISLAM K.B.M. Saiful; 大岡唯祐; 小椋義俊; 小椋義俊; 林哲也; 林哲也; 萩尾真人; 石塚敏, 日本農芸化学会大会講演要旨集(Web), 2014, 2014
• 消化管内で発現するビフィズス菌遺伝子の探索系の構築
  河口礼佳; 平等清夏; 阪中幹祥; 平山洋佑; 横田篤; 吹谷智, 日本乳酸菌学会誌, 25, 2, 119, 119, 2014
  日本乳酸菌学会, Japanese
• 消化管内特異的に発現するビフィズス菌遺伝子の探索系の構築
  平等清夏; 河口礼佳; 阪中幹祥; 平山洋佑; 横田篤; 吹谷智, 日本ゲノム微生物学会年会要旨集, 8th, 2014
• ビフィズス菌由来シアリダーゼのムチン付着因子としての評価
  西山啓太; 吹谷智; 横田篤; 山本裕司; 向井孝夫, 日本畜産学会大会講演要旨, 118th, 169, 169, 2014
  (公社)日本畜産学会, Japanese
• Skatoleの経口負荷が肝Cyp7a1発現と二次胆汁酸排泄の亢進を誘導する
  野勢琢馬; 清水英寿; 萩尾真人; 吹谷智; 横田篤; 原博; 石塚敏, 肥満研究, 20, Supplement, 2014
• ビフィズス菌のシアリダーゼは付着因子として機能する
  西山啓太; 吹谷智; 横田篤; 山本裕司; 向井孝夫, ミルクサイエンス, 63, 2, 106, 106, 2014
  日本酪農科学会, Japanese
• 腸内細菌代謝物スカトールは肝臓でのCyp7a1の発現と糞中二次胆汁酸増加を誘導する
  野勢琢馬; 清水英寿; 萩尾真人; 吹谷智; 横田篤; 原博; 石塚敏, 日本分子生物学会年会プログラム・要旨集(Web), 37th, 2014
• 2P-093 Effects of pdhR-deleted mutation on glucose metabolism in Escherichia coli
  Iwabu Yuki; Kihira Chie; Maeda Soya; Fukiya Satoru; Wada Masaru; Yokota Atsushi, 日本生物工学会大会講演要旨集, 65, 127, 127, 25 Aug. 2013
  The Society for Biotechnology, Japan, Japanese
• 日本農芸化学会大会2013年度シンポジウム「プロバイオティクスの機能評価と新展開-ヒトの健康と疾病予防を支える乳酸菌とビフィズス菌-」報告
  川井 泰; 吹谷 智, 日本乳酸菌学会誌 = Journal of Japan Society for Lactic Acid Bacteria, 24, 2, 147, 148, 20 Jun. 2013
  Japanese
• Oral administration of cholic acid modulates the gut microbiota and epithelial proliferation
  Satoshi Ishizuka; Manami Takatsuki; Maiko Shiwaku; Masahito Hagio; Reika Yoshitsugu; Keidai Kikuchi; Nobuyuki Fujii; Ga-Hyun Joe; Hidehisa Shimizu; Hiroshi Hara; Satoru Fukiya; Atsushi Yokota, FASEB JOURNAL, 27, Apr. 2013
  English, Summary international conference
• 微生物の共存・共生と相互作用 遺伝子変異導入法の確立により見えてきたビフィズス菌の腸管定着因子
  阪中幹祥; 吹谷智; 横田篤, 日本乳酸菌学会誌, 24, 1, 36, 36, 15 Mar. 2013
  日本乳酸菌学会, Japanese
• 乳酸菌のリポテイコ酸のイムノブロットによる検出方法の確立
  佐藤耶舞羽; 白石宗; 横田伸一; 吹谷智; 横田篤, 日本乳酸菌学会誌, 24, 2, 137, 137, 2013
  日本乳酸菌学会, Japanese
• 二重相同組換え法を用いたBifidobacterium longumの染色体上遺伝子への欠失変異導入技術の開発
  吹谷智; 平山洋佑; 阪中幹祥; 福間英訓; 村山寛樹; 加納康正; 横田篤, 日本ゲノム微生物学会年会要旨集, 7th, 2013
• 腸内特異的に発現するビフィズス菌遺伝子の探索系の構築
  平等清夏; 河口礼佳; 阪中幹祥; 平山洋佑; 吹谷智; 横田篤, 日本農芸化学会北海道支部講演会講演要旨, 2013, 2013
• 異種遺伝子発現への応用を目的としたビフィズス菌のプロモーターの評価
  玉井早紀; 阪中幹祥; 平山洋佑; 吹谷智; 横田篤, 日本農芸化学会北海道支部講演会講演要旨, 2013, 2013
• Lactobacillus gasseri JCM1131^Tに見出された新規なリポテイコ酸の構造
  白石宗; 横田伸一; 森田直樹; 吹谷智; 冨田理; 田中尚人; 岡田早苗; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2013, 2013
• プロバイオティクス機能の分子機構解明に向けたビフィズス菌の遺伝子欠損導入系の開発
  吹谷智; 平山洋佑; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2013, 2013
• Bifidobacterium longum105‐Aにおけるシステイン/メチオニン代謝の解析
  鈴木梓; 吹谷智; 横田篤; 和田大, 日本農芸化学会北海道支部講演会講演要旨, 2012, 20, 01 Nov. 2012
  Japanese
• 呼吸鎖酵素およびPdhRの欠失による大腸菌の糖代謝活性の変化
  荒井博紀; 紀平知枝; 前田壮矢; 吹谷智; 和田大; 横田篤, 日本生物工学会大会講演要旨集, 64th, 204, 204, 25 Sep. 2012
  日本生物工学会, Japanese
• 新規ウルソデオキシコール酸生成腸内細菌Ruminococcus gnavus N53由来の7β‐hydroxysteroid dehydrogenaseの発現と機能解析
  李慈英; 吹谷智; 和田大; 横田篤, 日本乳酸菌学会誌, 23, 2, 124, 01 Jul. 2012
  Japanese
• pdhR欠失変異が大腸菌の糖代謝に与える影響
  前田壮矢; 紀平知枝; 清水久美子; 吹谷智; 和田大; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2012, 3C20P14 (WEB ONLY), 05 Mar. 2012
  Japanese
• NADH dehydrogenase II過剰発現による大腸菌の糖代謝特性の変動
  紀平知枝; 林由香里; 吹谷智; 和田大; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2012, 3C20P15 (WEB ONLY), 05 Mar. 2012
  Japanese
• 新規ウルソデオキシコール酸生成腸内細菌Ruminococcus gnavus N53由来の7β‐hydroxysteroid dehydrogenaseの発現と機能解析
  LEE JaYoung; 荒井尚志; 吹谷智; 和田大; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2012, 3C09A11 (WEB ONLY), 05 Mar. 2012
  Japanese
• フマル酸添加が腸内細菌による胆汁酸変換に及ぼす影響
  本田希未; CHANG Hsin-Yu; 吹谷智; 石塚敏; 横田篤, 日本生物工学会大会講演要旨集, 64th, 208, 208, 2012
  (公社)日本生物工学会, Japanese
• 腸内乳酸菌Lactobacillus gasseri JCM1131^Tのリポテイコ酸の構造とコール酸適応への関与
  白石宗; 冨田理; 田中尚人; 吹谷智; 森田直樹; 横田伸一; 岡田早苗; 横田篤, 日本乳酸菌学会誌, 23, 2, 106, 106, 2012
  日本乳酸菌学会, Japanese
• ビフィズス菌由来挿入配列TLS143の転移機能の解析
  阪中幹祥; 阿部光紗; 平山洋佑; 吹谷智; 加納康正; 横田篤, 腸内細菌学雑誌, 26, 2, 111, 111, 2012
  (公財)腸内細菌学会, Japanese
• ランダム変異導入法への応用を目指したビフィズス菌由来挿入配列TLS143の機能解析
  阪中幹祥; 阿部光紗; 平山洋佑; 吹谷智; 加納康正; 横田篤, 日本ゲノム微生物学会年会要旨集, 6th, 2012
• 新規大腸菌-ビフィズス菌シャトルベクターの構築
  小野寺愛; 平山洋佑; 阪中幹祥; 吹谷智; 片山高嶺; 加納康正; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2012, 2012
• Lactobacillus gasseri JCM1131^Tのリポテイコ酸:胆汁酸適応への関与
  白石宗; 冨田理; 田中尚人; 吹谷智; 森田直樹; 横田伸一; 岡田早苗; 横田篤, 日本農芸化学会大会講演要旨集(Web), 2012, 2012
• ラット盲腸内細菌叢を制御する宿主因子としての胆汁酸の機能
  吹谷智; SAIFUL ISLAM K.B.M.; 萩尾真人; 藤井暢之; 石塚敏; 大岡唯祐; 大岡唯祐; 小椋義俊; 小椋義俊; 林哲也; 林哲也; 横田篤, 日本生物工学会大会講演要旨集, 64th, 219, 219, 2012
  (公社)日本生物工学会, Japanese
• 大腸菌の呼吸鎖改変による糖代謝変化に関する研究
  荒井博紀; 紀平知枝; 吹谷智; 和田大; 横田篤, 日本生体エネルギー研究会討論会講演要旨集, 38th, 21, 22, 2012
  Japanese
• 2Ha09 Effectiveness of pdhR deletion in glucose metabolism of Escherichia coli
  MAEDA Soya; KIHIRA Chie; SHIMIZU Kumiko; FUKIYA Satoru; WADA Masaru; YOKOTA Atsushi, 日本生物工学会大会講演要旨集, 0, 63, 168, 168, 25 Aug. 2011
  公益社団法人日本生物工学会, Japanese
• 大腸菌のpdhR欠失変異株の培養特性解析
  前田壮矢; 紀平知枝; 清水久美子; 吹谷智; 和田大; 横田篤, 日本農芸化学会大会講演要旨集, 2011, 90, 05 Mar. 2011
  Japanese
• 新規ウルソデオキシコール酸生成腸内細菌の反応特性の解析
  荒井尚志; 李慈英; 吹谷智; 和田大; 横田篤, 日本農芸化学会大会講演要旨集, 2011, 274, 05 Mar. 2011
  Japanese
• 呼吸鎖欠損が大腸菌の糖代謝に与える影響
  紀平知枝; 前田壮矢; 吹谷智; 和田大; 松下一信; 横田篤, 日本農芸化学会大会講演要旨集, 2011, 90, 05 Mar. 2011
  Japanese
• コール酸添加食摂取がラット盲腸内の細菌叢及び胆汁酸代謝に与える影響
  SAIFUL Islam K. B. M.; 吹谷智; 萩尾真人; 石塚敏; 横田篤, 日本農芸化学会大会講演要旨集, 2011, 2011
• Lactobacillus gasseri JCM1131における推定カルジオリピンシンターゼ遺伝子欠失がコール酸適応現象に与える影響
  戸部はるひ; 澤田真理子; 加藤慎二; 森田直樹; 佐々木泰子; 扇谷悟; 吹谷智; 横田篤, 日本農芸化学会大会講演要旨集, 2011, 2011
• ビフィズス菌の挿入配列を利用したランダム変異導入系の確立
  阿部光紗; 阪中幹祥; 小林諒子; 平山洋佑; 吹谷智; 加納康正; 横田篤, 日本農芸化学会大会講演要旨集, 2011, 2011
• Bifidobacterium longum105-Aのα-ガラクトシダーゼ遺伝子破壊株の性状解析とレポーターアッセイ系への応用
  平山洋佑; 福間英訓; 村山寛樹; 吹谷智; 加納康正; 横田篤, 日本乳酸菌学会誌, 22, 2, 124, 124, 2011
  日本乳酸菌学会, Japanese
• Bifidobacterium longum105-A由来挿入配列TLS143の挿入特異性および転移活性の解析
  阪中幹祥; 阿部光紗; 平山洋佑; 吹谷智; 加納康正; 横田篤, 日本乳酸菌学会誌, 22, 2, 124, 124, 2011
  日本乳酸菌学会, Japanese
• NADH dehydrogenase IIの過剰発現による大腸菌の糖代謝活性の増大および呼吸鎖欠損株への導入による糖代謝改善効果
  紀平知枝; 林由香里; 吹谷智; 和田大; 横田篤, 日本生体エネルギー研究会討論会講演要旨集, 37th, 96, 97, 2011
  Japanese
• 2P-1109 Optimization of culture conditions for physiological analysis of fermentation in Escherichia coli
  Kihira Chie; Maeda Soya; Fukiya Satoru; Wada Masaru; Yokota Atsushi, 日本生物工学会大会講演要旨集, 22, 0, 25 Sep. 2010
  公益社団法人日本生物工学会, Japanese
• 呼吸鎖欠損変異による大腸菌の糖代謝特性の変化
  紀平知枝; 吹谷智; 和田大; 松下一信; 横田篤, 日本農芸化学会大会講演要旨集, 2010, 205, 05 Mar. 2010
  Japanese
• 嫌気呼吸の促進によるラット腸管における発癌二次胆汁酸生成抑制の試み
  張シンユ; 萩尾真人; 吹谷智; 石塚敏; 横田篤, 日本農芸化学会大会講演要旨集, 2010, 2010
• 16S rRNA遺伝子クローンライブラリー法による胆汁酸添加食摂取ラット腸内細菌叢の解析
  SAIFUL ISLAM K. B. M.; 萩尾真人; 石塚敏; 吹谷智; 横田篤, 腸内細菌学雑誌, 24, 2, 2010
• 腸内乳酸菌Lactobacillus gasseri JCM1131^Tにおけるコール酸適応機構と細胞膜脂質組成変化の関連性
  加藤慎二; 森田直樹; 佐々木泰子; 扇谷悟; 吹谷智; 横田篤, 日本農芸化学会大会講演要旨集, 2010, 2010
• 胆汁酸代謝を介した生理作用評価のための実験系構築の試み
  藤井暢之; 萩尾真人; 安藤翠; ISLAM K. B. M. Saiful; 吹谷智; 横田篤; 原博; 石塚敏, 日本食物繊維学会誌, 14, 2, 2010
• Bifidobacterium longum105-A株における染色体遺伝子破壊技術の構築
  平山洋佑; 福間英訓; 村山寛樹; 吹谷智; 加納康正; 横田篤, 日本農芸化学会大会講演要旨集, 2010, 2010
• Bifidobacterium longum105-A株における染色体上の標的遺伝子破壊技術の構築
  平山洋佑; 福間英訓; 村山寛樹; 吹谷智; 加納康正; 横田篤, 日本乳酸菌学会誌, 21, 2, 2010
• 2Fa15 Enhancement of glucose metabolism by pdhR deletion in Escherichia coli
  Kihira Chie; Shimizu Kumiko; Fukiya Satoru; Wada Masaru; Yokota Atsushi, 日本生物工学会大会講演要旨集, 21, 0, 77, 77, 25 Aug. 2009
  公益社団法人日本生物工学会, Japanese
• Miscellaneous
  吹谷 智; 善藤 威史, 日本乳酸菌学会誌 = Journal of Japan Society for Lactic Acid Bacteria, 20, 1, 16, 17, 15 Mar. 2009
  Japanese
• 新規二次胆汁酸生成菌Bacteroides intestinalis AM‐1の7‐oxo胆汁酸の生成特性について
  川嶋紘子; 荒田美紀; 吹谷智; 和田大; 横田篤, 日本農芸化学会大会講演要旨集, 2009, 124, 05 Mar. 2009
  Japanese
• 新規ウルソデオキシコール酸生成腸内細菌Ruminococcus gnavus N53の分離および本株の胆汁酸変換反応の解析
  中村勇介; 荒田美紀; 吹谷智; 和田大; 横田篤, 日本農芸化学会大会講演要旨集, 2009, 123, 05 Mar. 2009
  Japanese
• 呼吸鎖欠損変異による大腸菌の糖代謝活性の向上
  紀平知枝; 吹谷智; 和田大; 横田篤, 日本農芸化学会大会講演要旨集, 2009, 261, 05 Mar. 2009
  Japanese
• 腸内乳酸菌Lactobacillus gasseri JCM1131^Tにおけるコール酸適応機構の解明
  加藤慎二; 澤田真理子; 松原裕樹; 森田直樹; 佐々木泰子; 扇谷悟; 吹谷智; 横田篤, 日本農芸化学会大会講演要旨集, 2009, 2009
• 腸内乳酸菌Lactobacillus gasseri JCM1131^Tにおけるコール酸適応機構と細胞膜脂質組成変化の関連性
  加藤慎二; 澤田真理子; 松原裕樹; 森田直樹; 佐々木泰子; 扇谷悟; 吹谷智; 横田篤, 日本乳酸菌学会誌, 20, 2, 70, 70, 2009
  日本乳酸菌学会, Japanese
• ラフィノースのビフィズス菌増殖促進効果とプレバイオティクス研究の方向性
  しょく田篤; DINOTO Achmad; 吹谷智; 原博; 石塚敏, 日本栄養・食糧学会大会講演要旨集, 63rd, 41, 41, 2009
  (公社)日本栄養・食糧学会, Japanese
• 呼吸鎖欠損変異が大腸菌の糖代謝に与える影響
  紀平知枝; 吹谷智; 和田大; 横田篤, 日本生体エネルギー研究会討論会講演要旨集, 35th, 33, 34, 2009
  Japanese
• 急展開を見せる腸内細菌研究 : 大規模シークエンス解析により明らかになってきた腸内細菌叢の構造と機能
  吹谷 智; 横田 篤, 化学と生物, 47, 1, 11, 13, 01 Jan. 2009
  社団法人 日本農芸化学会, Japanese
• 腸内でのメタン生成菌の役割(バイオミディア)
  吹谷 智, 生物工学会誌 : seibutsu-kogaku kaishi, 86, 10, 492, 492, 25 Oct. 2008
  公益社団法人日本生物工学会, Japanese
• 腸内乳酸菌Lactobacillus gasseri JCM1131^Tにおけるコール酸適応機構の解明
  松原裕樹; 森田直樹; 佐々木泰子; 扇谷悟; 吹谷智; 横田篤, 日本農芸化学会大会講演要旨集, 2008, 2008
• 新たな腸内細菌叢解析手法としてのFISH-フローサイトメトリー法
  吹谷智; 横田篤, 生化学, 80, 5, 421, 425, 2008
  (公社)日本生化学会, Japanese
• Bifidobacterium longumからの転位活性を持つ挿入配列の単離
  吹谷智; 小林諒子; 加納康正; 横田篤, 日本乳酸菌学会誌, 19, 2, 113, 113, 2008
  日本乳酸菌学会, Japanese
• ヒト腸内フローラの迅速・網羅的解析法の開発
  辻浩和; 松田一乗; 朝原崇; 松本一政; 松木隆広; 野本康二; 吹谷智; 横田篤, 日本農芸化学会大会講演要旨集, 2008, 2008
• ビフィズス菌からの転位能をもつ挿入配列の単離と同定
  小林諒子; 吹谷智; 加納康正; 横田篤, 日本農芸化学会大会講演要旨集, 2008, 2008
• FISH-フローサイトメトリー法によるヒト腸管内ビフィズス菌動態のモニタリング
  横田篤; 吹谷智, 日本微生物生態学会講演要旨集, 24th, 2008
• プロテオーム解析によるLactobacillus gasseri JCM 1131^Tのコール酸適応機構の解明
  平野友美; 加藤慎二; 佐々木泰子; 吹谷智; 横田篤, 日本農芸化学会大会講演要旨集, 2008, 2008
• Oenococcus oeni由来プラスミドpOM1に存在するORF3は酸耐性に関与するレギュレーター様タンパク質をコードする
  小田美也子; 澤渡優喜; 吹谷智; 横田篤; 池田隆幸, 日本農芸化学会大会講演要旨集, 2008, 2008
• 新規二次胆汁酸生成菌Bacteroides intestinalis AM‐1の二次胆汁酸生成能について
  荒田美紀; 吹谷智; 和田大; 横田篤, 日本農芸化学会大会講演要旨集, 2007, 165, 05 Mar. 2007
  Japanese
• 腸内乳酸菌Lactobacillus gasseri JCM1131^Tにおけるコール酸適応機構の解明
  松原裕樹; 森田直樹; 佐々木泰子; 扇谷悟; 吹谷智; 横田篤, 日本農芸化学会北海道支部・日本土壌肥料学会北海道支部・日本生物工学会北日本支部・日本応用糖質科学会北海道支部・北海道農芸化学協会合同学術講演会講演要旨, 2007, 2007
• ビフィズス菌Bifidobacterium longum内在性プラスミドからの挿入配列の同定と塩基配列解析
  吹谷智; 杉山友彦; 加納康正; 横田篤, 日本乳酸菌学会誌, 18, 2, 82, 82, 2007
  日本乳酸菌学会, Japanese
• Bifidobacterium longum内在性プラスミドからの挿入配列の同定と塩基配列解析
  吹谷智; 杉山友彦; 加納康正; 横田篤, 日本生物工学会大会講演要旨集, 59th, 119, 119, 2007
  (公社)日本生物工学会, Japanese
• FISH-フローサイトメトリー法によるラフィノース摂取ヒト糞便における種レベルでのビフィズス菌動態解析
  吹谷智; DINOTO Achmad; TATIANA Marques; 渡辺純; 伊藤進; 横田篤, 日本農芸化学会大会講演要旨集, 2007, 2007
• ヒト糞便から新規二次胆汁酸生成菌の分離と同定
  荒田美紀; 南田公子; 吹谷智; 和田大; 小倉嘉夫; 伊藤喜久治; 内田清久; 横田篤, 日本農芸化学会大会講演要旨集, 2006, 244, 05 Mar. 2006
  Japanese
• Lactobacillus gasseri JCM1131^Tにおけるコール酸適応現象
  松原裕樹; 水谷佳奈子; 森田直樹; 佐々木泰子; 扇谷悟; 吹谷智; 横田篤, 日本農芸化学会大会講演要旨集, 2006, 2006
• イネいもち病菌の非病原性遺伝子Avr-Piaのクローニング
  三木慎介; 松井幸太郎; 鬼頭英樹; 吹谷智; 佐藤順子; 平八重一之; 中島敏彦; 冨田房男; 曾根輝雄, 日本農芸化学会大会講演要旨集, 2006, 2006
• FISH-フローサイトメトリー法を用いたラフィノース摂取ヒト糞便におけるビフィズス菌の動態解析
  ACHMAD Dinoto; Marques Tatiana; 吹谷智; 渡辺純; 伊藤進; 横田篤, 日本農芸化学会大会講演要旨集, 2006, 2006
• How many common genes are there in Escherichia coli? Probing the diversity of E. coli genome through comparative genome analysis using a microarray.
  吹谷智; 森英郎, 化学と生物, 42, 9, 2004
• 腸炎エルシニアのATP依存型プロテアーゼLonによる病原性発現制御機構の解析
  吹谷智; 中林夏子; 高屋明子; 友安俊文; 中根明夫; 山本友子, 日本分子生物学会年会プログラム・講演要旨集, 27th, 2004
• 経口サルモネラワクチンによるリステリアに対する感染防御効果について
  児玉千絵; 松井英則; 江口正浩; 吹谷智; 高屋明子; 友安俊文; 村山そう明; 生方公子; 山本友子, 日本細菌学雑誌, 59, 1, 200, 200, 2004
  日本細菌学会, Japanese
• Cre/loxP系を用いた大腸菌染色体大規模欠失法の確立
  吹谷智; 溝口寛; 森英郎, 日本農芸化学会大会講演要旨集, 2003, 2003
• (75)Molecular Cloning of the Avirulence Gene Avr-pia from Magnaporthe grisea by Utilizing of the Host Specificity Mutant
  Kito H.; Takahashi Y.; Fukiya S.; Sato J.; Sone T.; Iwano M.; Nakajima T.; Tomita F., Annals of the Phytopathological Society of Japan, 68, 2, 176, 176, 25 Aug. 2002
  The Phytopathological Society of Japan (PSJ), Japanese
• (76)A New DNA-Type Transposon Occan of Magnaporthe grisea
  Takahashi Y.; Kito H.; Sato J.; Fukiya S.; Sone T.; Tomita F., Annals of the Phytopathological Society of Japan, 68, 2, 176, 176, 25 Aug. 2002
  The Phytopathological Society of Japan (PSJ), Japanese
• 変異株を利用したいもち病菌非病原性遺伝子の取得
  鬼頭英樹; 高橋洋介; 吹谷智; 佐藤順子; 岩野正敬; 曽根輝雄; 冨田房男, 日本農芸化学会大会講演要旨集, 2002, 2002
• Identification and Cloning of a Putative Serine Protease Gene from the Rice Blast Fungus, Magnaporthe grisea
  Fukiya S.; Kuge T.; Tanishima T.; Sone T.; Kamakura T.; Yamaguchi I.; Tomita F., Annals of the Phytopathological Society of Japan, 67, 2, 131, 132, 25 Aug. 2001
  The Phytopathological Society of Japan (PSJ), Japanese
• 変異株を利用したいもち病菌非病原性遺伝子連鎖マーカーの取得
  鬼頭英樹; 吹谷智; 佐藤順子; 谷島智美; 岩野正敬; 曽根輝雄; 冨田房男, 日本農芸化学会誌, 75, 2001
• (175) Analysis of the Deleted Region of Host Specificity Mutants
  Sato J.; Fukiya S.; Sone T.; Iwano M.; Tomita F., Annals of the Phytopathological Society of Japan, 64, 4, 370, 370, 25 Aug. 1998
  The Phytopathological Society of Japan (PSJ), Japanese
• (173) DNA Finger printing of Japanese Isolates of Rice Blast Fungus
  Sone T.; Fukiya S.; Iwano M.; Koizumi S.; Fujita Y.; Tomita F., Annals of the Phytopathological Society of Japan, 64, 4, 370, 370, 25 Aug. 1998
  The Phytopathological Society of Japan (PSJ), Japanese
• (215) Establishment of a New Cross System for Japanese Rice Pathogens of Rice Blast Fungus(Abstracts of the Papers Presented at the Annual Meeting of the Society)
  FUKIYA S.; SONE T.; ABE T.; KODAMA M.; SUTO M.; TOMITA F., Annals of the Phytopathological Society of Japan, 63, 3, 244, 245, 25 Jun. 1997
  The Phytopathological Society of Japan (PSJ), Japanese
• A Molecular Genetic Approach to Pathogenic-race Variation of Rice Blast Fungus
  SONE T.; ABE T.; FUKIYA S.; KODAMA M.; IWANO M.; KOIZUMI S.; FUJITA Y.; TOMITA F., Annals of the Phytopathological Society of Japan, 62, 3, 294, 294, 25 Jun. 1996
  The Phytopathological Society of Japan (PSJ), Japanese
• いもち病菌の高稔性交配系を用いた遺伝子解析 : 微生物
  曽根 輝雄; 吹谷 智; 阿部 卓巳; 児玉 恵; 須藤 学; 冨田 房男, 日本農藝化學會誌, 70, 117, 117, 05 Mar. 1996
  社団法人日本農芸化学会, Japanese
• イネいもち病菌による交配実験系の確立 : 微生物
  曽根 輝雄; 吹谷 智; 阿部 卓巳; 吉田 奈美; 児玉 恵; 須藤 学; 富田 房男, 日本農藝化學會誌, 69, 273, 273, 05 Jul. 1995
  社団法人日本農芸化学会, Japanese

• The bifidobacteria and related organisms : biology, taxonomy, applications
  Mattarelli, Paola; Biavati, Bruno; Holzapfel, W. H.; Wood, Brian J. B., 15. Genetic Manipulation and Gene Modification Technologies in Bifidobacteria
  Academic Press, an imprint of Elsevier, 2018, 9780128050606, xii, 312 p., English, [Contributor]
• Lactic Acid Bacteria: Current Progress in Advanced Research
  Horizon Scientific Press, 2011
• 乳酸菌とビフィズス菌のサイエンス
  京都大学学術出版会, 2010

• Comprehensive identification of bifidobacterial genes contributing to intestinal survival by insertion sequencing.
  Satoru Fukiya
  The 1st International Symposium on Bifidobacterium Research, 12 Oct. 2024, English, Invited oral presentation
  34027839;48260996, [Invited]
• Identification and characterization of bifidobacterial genes contributing to survival in the intestine by insertion sequencing.
  Satoru Fukiya; Atsushi Yokota
  The 51th Naito Conference on Microbiome in Health and Disease., English, Poster presentation
  25 Jun. 2024 - 28 Jun. 2024, 34027839;48260996
• Insertion Sequencing法によるビフィズス菌の腸内生存に寄与する遺伝子の同定
  吹谷 智
  第95回⽇本細菌学会総会ワークショップ, 30 Mar. 2022, Japanese, Public symposium
  34027839;34027837, [Invited]
• ビフィズス菌の腸内生存機構の解明に向けた遺伝子操作ツールの応用
  吹谷 智
  第一回ビフィズス菌研究会シンポジウム, 12 Mar. 2021, Japanese
  34027839;10587211, [Invited]
• 腸内での二次胆汁酸生成における腸内細菌間相互作用
  吹谷 智
  2018年度日本生物工学会北日本支部札幌シンポジウム, 05 Oct. 2018, Japanese, Nominated symposium
  [Invited], [Domestic Conference]
• Development of two gene-mutagenesis systems in Bifidobacterium longum
  FUKIYA Satoru; HIRAYAMA Yosuke; SAKANAKA Mikiyasu; YOKOTA Atsushi
  5th AFSLAB International Symposium, 28 Nov. 2016, English, Invited oral presentation
  [Invited], [International presentation]
• 西欧食による腸内細菌叢崩壊のメカニズム：胆汁酸仮説の検討．
  吹谷 智; 渡辺 真通; 石塚 敏; 横田 篤
  日本農芸化学会創立100周年に向けたシンポジウム（Visionary 農芸化学100シンポジウム）第一回「食、腸内細菌、健康」, 02 Oct. 2016, Japanese, Nominated symposium
  [Invited], [Domestic Conference]
• Development of practical gene-mutagenesis systems in Bifidobacterium longum
  FUKIYA Satoru; HIRAYAMA Yosuke; SAKANAKA Mikiyasu; YOKOTA Atsushi
  PropioBifido 2016, 21 Sep. 2016, English, Invited oral presentation
  [Invited], [International presentation]
• 腸内細菌と健康 －西欧食による腸内細菌叢バランスの変化と疾病発症－
  吹谷 智; 石塚 敏; 横田 篤
  日本機能水学会 第15回学術大会, 10 Sep. 2016, Japanese, Invited oral presentation
  [Invited], [Domestic Conference]
• ビフィズス菌の２つの遺伝子変異導入系の確立：標的遺伝子への欠失変異導入系とトランスポゾン変異導入系．
  吹谷 智
  平成28年度 日本農芸化学会 北海道支部第1回講演会, 06 Aug. 2016, Japanese, Invited oral presentation
  [Invited], [Domestic Conference]
• Development of practical gene-mutagenesis systems in bifidobacteria.
  FUKIYA Satoru
  2016 KSBB Spring Meeting, 22 Apr. 2016, English, Invited oral presentation
  [Invited], [International presentation]
• 2A-Cp01 Development of practical gene-mutagenesis systems in bifidobacteria
  Fukiya Satoru
  日本生物工学会大会講演要旨集, 25 Sep. 2015, Japanese
  [Invited]
• 2P-023 Development of a screening system for Bifidobacterium genes specifically expressed in vivo
  Koguchi Hiroka; Hirato Sayaka; Sakanaka Mikiyasu; Yokota Atsushi; Fukiya Satoru
  日本生物工学会大会講演要旨集, 05 Aug. 2014, Japanese
• 1P-117 Effect of fumarate on the cholic acid metabolism in Clostridium hiranonis
  Torii Tsuyoshi; Honda Nozomi; Hagio Masahito; Fukiya Satoru; Yokota Atsushi
  日本生物工学会大会講演要旨集, 05 Aug. 2014, Japanese
• 1P-116 Analysis of S-adenosylmethionine cycle involved in methionine utilization in bifidobacteria
  Komatsu Kazuki; Suzuki Azusa; Sakanaka Mikiyasu; Fukiya Satoru; Yokota Atsushi; Wada Masaru
  日本生物工学会大会講演要旨集, 05 Aug. 2014, Japanese
• 1P-115 Effects of respiratory-chain-enzyme defective mutations on central carbon metabolism in Escherichia coli
  KATOH Ryuichi; ARAI Hironori; KIHIRA Chie; FUKIYA Satoru; WADA Masaru; YOKOTA Atsushi
  日本生物工学会大会講演要旨集, 05 Aug. 2014, Japanese
• 胆汁酸による腸内細菌叢の動態制御とそれに関連する疾患の発症
  吹谷 智; Islam KBM Saiful; Ja-Young Lee; 萩尾 真; 清水 英寿; 石塚 敏; 大岡 唯祐; 小椋 義俊; 林 哲也; 横田 篤
  ゲノム創薬・医療フォーラム第1回談話会, 18 Jun. 2014, Japanese, Nominated symposium
  [Invited], [Domestic Conference]
• 異種遺伝子発現への応用を目的としたビフィズス菌のプロモーターの評価
  玉井早紀; 阪中幹祥; 平山洋佑; 吹谷智; 横田篤
  日本農芸化学会北海道支部講演会講演要旨, 27 Nov. 2013, Japanese
• 腸内特異的に発現するビフィズス菌遺伝子の探索系の構築
  平等清夏; 河口礼佳; 阪中幹祥; 平山洋佑; 吹谷智; 横田篤
  日本農芸化学会北海道支部講演会講演要旨, 27 Nov. 2013, Japanese
• 2P-093 Effects of pdhR-deleted mutation on glucose metabolism in Escherichia coli
  Iwabu Yuki; Kihira Chie; Maeda Soya; Fukiya Satoru; Wada Masaru; Yokota Atsushi
  日本生物工学会大会講演要旨集, 25 Aug. 2013, Japanese
• 大腸菌におけるpdhR欠失変異が糖代謝に及ぼす影響について
  岩部有起; 紀平知枝; 前田壮矢; 吹谷智; 和田大; 横田篤
  日本生物工学会大会講演要旨集, 25 Aug. 2013, Japanese
• 乳酸菌のリポテイコ酸のイムノブロットによる検出方法の確立
  佐藤耶舞羽; 白石宗; 横田伸一; 吹谷智; 横田篤
  日本乳酸菌学会誌, 20 Jun. 2013, Japanese
• プロバイオティクス機能の分子機構解明に向けたビフィズス菌の遺伝子欠損導入系の開発
  吹谷 智; 平山洋佑; 横田 篤
  日本農芸化学会2013年度大会シンポジウム, 14 Mar. 2013, Japanese, Public symposium
  [Invited], [Domestic Conference]
• プロバイオティクス機能の分子機構解明に向けたビフィズス菌の遺伝子欠損導入系の開発
  吹谷智; 平山洋佑; 横田篤
  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2013, Japanese
• Lactobacillus gasseri JCM1131^Tに見出された新規なリポテイコ酸の構造
  白石宗; 横田伸一; 森田直樹; 吹谷智; 冨田理; 田中尚人; 岡田早苗; 横田篤
  日本農芸化学会大会講演要旨集(Web), 05 Mar. 2013, Japanese
• 二重相同組換え法を用いたBifidobacterium longumの染色体上遺伝子への欠失変異導入技術の開発
  吹谷智; 平山洋佑; 阪中幹祥; 福間英訓; 村山寛樹; 加納康正; 横田篤
  日本ゲノム微生物学会年会要旨集, 2013, Japanese
• Bifidobacterium longum105‐Aにおけるシステイン/メチオニン代謝の解析
  鈴木梓; 吹谷智; 横田篤; 和田大
  日本農芸化学会北海道支部講演会講演要旨, 01 Nov. 2012, Japanese
• 呼吸鎖酵素およびPdhRの欠失による大腸菌の糖代謝活性の変化
  荒井博紀; 紀平知枝; 前田壮矢; 吹谷智; 和田大; 横田篤
  日本生物工学会大会講演要旨集, 25 Sep. 2012, Japanese
• フマル酸添加が腸内細菌による胆汁酸変換に及ぼす影響
  本田希未; CHANG Hsin‐Yu; 吹谷智; 石塚敏; 横田篤
  日本生物工学会大会講演要旨集, 25 Sep. 2012, Japanese
• ラット盲腸内細菌叢を制御する宿主因子としての胆汁酸の機能
  吹谷智; SAIFUL ISLAM K.B; M; 萩尾真人; 藤井暢之; 石塚敏; 大岡唯祐; 大岡唯祐; 小椋義俊; 小椋義俊; 林哲也; 林哲也; 横田篤
  日本生物工学会大会講演要旨集, 25 Sep. 2012, Japanese
• 新規ウルソデオキシコール酸生成腸内細菌Ruminococcus gnavus N53由来の7β‐hydroxysteroid dehydrogenaseの発現と機能解析
  李慈英; 吹谷智; 和田大; 横田篤
  日本乳酸菌学会誌, 01 Jul. 2012, Japanese
• 腸内乳酸菌Lactobacillus gasseri JCM1131^Tのリポテイコ酸の構造とコール酸適応への関与
  白石宗; 冨田理; 田中尚人; 吹谷智; 森田直樹; 横田伸一; 岡田早苗; 横田篤
  日本乳酸菌学会誌, 01 Jul. 2012, Japanese
• ビフィズス菌由来挿入配列TLS143の転移機能の解析
  阪中幹祥; 阿部光紗; 平山洋佑; 吹谷智; 加納康正; 横田篤
  腸内細菌学雑誌, Apr. 2012, Japanese
• ランダム変異導入法への応用を目指したビフィズス菌由来挿入配列TLS143の機能解析
  阪中幹祥; 阿部光紗; 平山洋佑; 吹谷智; 加納康正; 横田篤
  日本ゲノム微生物学会年会要旨集, 2012, Japanese
• 大腸菌の糖代謝におけるpdhR欠失の有効性について
  前田壮矢; 紀平知枝; 清水久美子; 吹谷智; 和田大; 横田篤
  日本生物工学会大会講演要旨集, 25 Aug. 2011, Japanese
• 2Ha09 Effectiveness of pdhR deletion in glucose metabolism of Escherichia coli
  MAEDA Soya; KIHIRA Chie; SHIMIZU Kumiko; FUKIYA Satoru; WADA Masaru; YOKOTA Atsushi
  日本生物工学会大会講演要旨集, 25 Aug. 2011, Japanese
• Bifidobacterium longum105‐Aのα‐ガラクトシダーゼ遺伝子破壊株の性状解析とレポーターアッセイ系への応用
  平山洋佑; 福間英訓; 村山寛樹; 吹谷智; 加納康正; 横田篤
  日本乳酸菌学会誌, 01 Jul. 2011, Japanese
• Bifidobacterium longum105‐A由来挿入配列TLS143の挿入特異性および転移活性の解析
  阪中幹祥; 阿部光紗; 平山洋佑; 吹谷智; 加納康正; 横田篤
  日本乳酸菌学会誌, 01 Jul. 2011, Japanese
• 2P-1109 Optimization of culture conditions for physiological analysis of fermentation in Escherichia coli
  Kihira Chie; Maeda Soya; Fukiya Satoru; Wada Masaru; Yokota Atsushi
  日本生物工学会大会講演要旨集, 25 Sep. 2010, Japanese
• 2Fa15 Enhancement of glucose metabolism by pdhR deletion in Escherichia coli
  Kihira Chie; Shimizu Kumiko; Fukiya Satoru; Wada Masaru; Yokota Atsushi
  日本生物工学会大会講演要旨集, 25 Aug. 2009, Japanese
• S-B1 Monitoring of population dynamics of bifidobacteria in human intestine by FISH-flow cytometry
  横田 篤; 吹谷 智
  日本微生物生態学会講演要旨集, 25 Nov. 2008, Japanese
• 2E11-3 Identification and characterization of a putative insertion sequence in a cryptic plasmid from Bifidobacterium longum
  FUKIYA Satoru; SUGIYAMA Tomohiko; KANO Yasunobu; YOKOTA Atsushi
  日本生物工学会大会講演要旨集, 02 Aug. 2007, Japanese
• 「大腸菌」に共通な遺伝子の数は? : マイクロアレイを用いた比較ゲノム解析から大腸菌ゲノムの多様性を探る
  吹谷 智; 森 英郎
  化学と生物, 25 Sep. 2004, Japanese
• (75)Molecular Cloning of the Avirulence Gene Avr-pia from Magnaporthe grisea by Utilizing of the Host Specificity Mutant
  Kito H; Takahashi Y; Fukiya S; Sato J; Sone T; Iwano M; Nakajima T; Tomita F
  Annals of the Phytopathological Society of Japan, 25 Aug. 2002, Japanese
• (76)A New DNA-Type Transposon Occan of Magnaporthe grisea
  Takahashi Y; Kito H; Sato J; Fukiya S; Sone T; Tomita F
  Annals of the Phytopathological Society of Japan, 25 Aug. 2002, Japanese
• Identification and Cloning of a Putative Serine Protease Gene from the Rice Blast Fungus, Magnaporthe grisea
  Fukiya S; Kuge T; Tanishima T; Sone T; Kamakura T; Yamaguchi I; Tomita F
  Annals of the Phytopathological Society of Japan, 25 Aug. 2001, Japanese
• (173) DNA Finger printing of Japanese Isolates of Rice Blast Fungus
  Sone T; Fukiya S; Iwano M; Koizumi S; Fujita Y; Tomita F
  Annals of the Phytopathological Society of Japan, 25 Aug. 1998, Japanese
• (175) Analysis of the Deleted Region of Host Specificity Mutants
  Sato J; Fukiya S; Sone T; Iwano M; Tomita F
  Annals of the Phytopathological Society of Japan, 25 Aug. 1998, Japanese
• (215) Establishment of a New Cross System for Japanese Rice Pathogens of Rice Blast Fungus(Abstracts of the Papers Presented at the Annual Meeting of the Society)
  FUKIYA S; SONE T; ABE T; KODAMA M; SUTO M; TOMITA F
  Annals of the Phytopathological Society of Japan, 25 Jun. 1997, Japanese
• A Molecular Genetic Approach to Pathogenic-race Variation of Rice Blast Fungus
  SONE T; ABE T; FUKIYA S; KODAMA M; IWANO M; KOIZUMI S; FUJITA Y; TOMITA F
  Annals of the Phytopathological Society of Japan, 25 Jun. 1996, Japanese
• いもち病菌の高稔性交配系を用いた遺伝子解析 : 微生物
  曽根 輝雄; 吹谷 智; 阿部 卓巳; 児玉 恵; 須藤 学; 冨田 房男
  日本農藝化學會誌, 05 Mar. 1996, Japanese
• イネいもち病菌による交配実験系の確立 : 微生物
  曽根 輝雄; 吹谷 智; 阿部 卓巳; 吉田 奈美; 児玉 恵; 須藤 学; 富田 房男
  日本農藝化學會誌, 05 Jul. 1995, Japanese

• 国際農学特論Ⅰ, 2024年, 博士後期課程, 農学院
• 大学院共通授業科目（一般科目）：自然科学・応用科学, 2024年, 修士課程, 大学院共通科目
• 国際農学特論Ⅱ, 2024年, 博士後期課程, 農学院
• 胃腸内圏微生物学特論, 2024年, 修士課程, 農学院
• 大学院共通授業科目（一般科目）：自然科学・応用科学, 2024年, 修士課程, 大学院共通科目
• 食品安全・機能性開発学特論, 2024年, 修士課程, 農学院
• 食品安全・機能性開発学特論演習, 2024年, 修士課程, 農学院
• 環境と人間, 2024年, 学士課程, 全学教育
• 教科教育法（農業Ⅰ）, 2024年, 学士課程, 教育学部
• 教科教育法（農業Ⅱ）, 2024年, 学士課程, 教育学部
• 応用菌学, 2024年, 学士課程, 農学部
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• 卒業論文, 2024年, 学士課程, 農学部
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• 微生物化学, 2024年, 学士課程, 農学部
• 生物学実験Ⅱ, 2024年, 学士課程, 農学部

• American society for microbiology
• 腸内細菌学会
• 日本ゲノム微生物学会
• 日本農芸化学会
• 日本乳酸菌学会
• 日本生物工学会

• Elucidation of the intestinal survival strategy of bifidobacteria through the coordination of amino acid biosynthesis and acquisition
  Grants-in-Aid for Scientific Research
  Apr. 2024 - Mar. 2028
  吹谷 智; 高須賀 太一
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Principal investigator, 24K01658
• 腸内細菌代謝産物に焦点を当てた高食肉摂取による健康増進と病態発症の分岐点の解明
  科学研究費助成事業
  01 Apr. 2021 - 31 Mar. 2025
  清水 英寿; 石塚 敏; 橋口 亜由未; 吹谷 智; 吉清 恵介; 田中 愛健
  培養肝がん細胞を用いた解析では、スカトールはAhRだけでなくPXRにも作用して、その代表的な標的遺伝子の1つであるCYP3A4の発現増加を導いた。このためスカトールは、培養肝がん細胞に対してPXRを介した作用も発揮することが示された。
  培養大腸がん細胞に対するスカトールの作用について、以前にCaco-2細胞を用いて得られた結果では、AhRの活性化を介して細胞の機能不全に導くことを報告した。今回、異なる培養大腸がん細胞であるHCT-116細胞を用いて解析を行ったところ、AhRの活性化を介して細胞増殖を誘導した。同様に、インドール酢酸やスカトールと異なるインドール系化合物の一種であるインドキシル硫酸は、AhRの活性化を介してHCT-116細胞の増殖を引き起こした。加えてAktの活性化もインドキシル硫酸によるHCT-116細胞の増殖に関与していた。以上から、同じ培養大腸がん細胞であっても、種類が異なることで、AhRの作用に違いが生じることが示された。
  Caco-2細胞を用いて、インドール酢酸とスカトールのクロストークについても解析を行った。インドール酢酸とスカトールは共にCYP1A1のタンパク量を増加させるものの、スカトールによるp38の活性化は、スカトール自身およびインドール酢酸によるCYP1A1のタンパク増加を抑制した。またインドール酢酸は、スカトールによるp38の活性化を一部抑制し、その結果としてスカトールが引き起こす効果を減弱させることが示唆された。よって、インドール酢酸からスカトールへの代謝過程において、インドール酢酸とスカトールの比が、大腸上皮細胞の恒常性に影響を及ぼす可能性が示された。
  日本学術振興会, 基盤研究(B), 島根大学, 23K21620
• 腸内細菌代謝産物に焦点を当てた高食肉摂取による健康増進と病態発症の分岐点の解明
  科学研究費助成事業 基盤研究(B)
  01 Apr. 2021 - 31 Mar. 2025
  清水 英寿; 石塚 敏; 橋口 亜由未; 吹谷 智; 吉清 恵介; 田中 愛健
  本研究では、「腸内細菌代謝産物に焦点を当てた高食肉摂取による健康増進と病態発症の分岐点の解明」に向け、腸内で産生されるインドール系化合物の一種、インドール酢酸とその代謝産物であるスカトールを中心に、肝臓および腸管への影響について解析を行っている。
  培養肝がん細胞を用いた解析では、スカトールは、その受容体として報告されているAryl hydrocarbon receptor （AhR）の活性化を介するだけでなく、ある核内受容体型転写因子の活性化も介して細胞増殖を導くことが明らかとなった。また我々は、スカトールによって発現増加する長寿遺伝子の1つであるSirt1が、培養肝がん細胞の増殖を導く結果を得た。
  培養大腸がん細胞を用いた解析では、スカトールは、Nuclear factor-kappa B（NF-kappaB）の活性化を介してInterleukin-6（IL-6）の発現増加を導いた。またスカトールは、Tumor necrosis factor alpha（TNFalpha）を発現増加させる一方、インドール酢酸に関しては、その発現低下を導くことを我々は報告している。このTNFalphaの発現低下メカニズムとして、TLR4が関与していることが示唆された。さらにインドール酢酸は、AhRの活性化を介して、腸管上皮内腔膜の塩化物-炭酸水素交換体としてNa+/H+交換体とともに腸管での電気陰性NaCl吸収に関与しているsolute-linked carrier 26（SLC26A3）の発現増加を誘導することが明らかとなった。SLC26A3の発現低下は、炎症性腸疾患や大腸癌で観察されることから、インドール酢酸はこれら病態に対する予防および改善に寄与すると考えられる。
  日本学術振興会, 基盤研究(B), 島根大学, 21H03356
• Elucidation of the gene expression profile of bifidobacteria in the gut microbiota: development of selective recovery method
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
  01 Apr. 2020 - 31 Mar. 2023
  吹谷 智; 後藤 恭宏
  1. 細胞表層にペプチドタグを提示したBifidobacterium longum 105-A株の構築
  ビフィズス菌の糖質ABCトランスポーターの基質結合タンパク質であるGltAのC末端側（細胞外へと露出する）に，選択的な回収に必要な4種類の候補ペプチドタグ（Strep-TagII, FLAG, HA, Myc）を付加したタグ融合GltAを発現可能なプラスミドを構築した．これらをB. longum 105-A株に導入し，4種類の表層タグ発現株を構築した．
  2. 表層タグ発現105-A株の培養菌体を用いた回収方法の検討
  ①RNA固定化の処理方法の検討とRNAの品質評価：市販のRNA固定化処理試薬を始めとした4種のRNA固定化溶液をテストした．培養菌体に固定化処理を施したのち，Total RNAを抽出し，バイオアナライザー(Agilent) を用いてRNAの品質（分解の度合）を評価した．その結果，RNAprotect Bacteria Reagent (QIAGEN) またはBacterial isolation buffer (Nobori et al., PNAS, 2018)を用いることで，RNAの品質を高く保つことができることが明らかになった．
  ②表層タグ発現株におけるタグ発現の検証：培養した表層タグ発現株菌体の全タンパク質に対して，各タグに特異的に結合する分子または抗体を用いて，ウェスタンブロット解析を行った．その結果，4種類のタグ全てがGltAとの融合タンパク質として発現していることが確認された．次に，表層タグ発現株を培養し，免疫蛍光染色を行い，蛍光顕微鏡を用いて細胞表層へのタグの提示を評価した．その結果，Strep-TagII発現株が最も高いタグ検出率を示したことから，4種の中でStrep-TagIIが最も高い割合で細胞表層へ提示されていることが明らかになった．
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 20H02893
• Elucidation of sulfur-assimilation mechanism in bifidobacteria
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
  01 Apr. 2020 - 31 Mar. 2023
  和田 大; 吹谷 智
  ビフィズス菌は広くCys要求性であると考えられてきたが，これまでの研究から，Bifidobacterium lomgum subsp. longum 105-A（105-A株）をはじめとする複数の菌株のCys要求性は，Metで代替可能であることを明らかにしている．これらの菌株はMetからCysへ代謝する逆流硫黄経路を有することが推定される．本経路は，(I) Metからホモシステイン(Hcy) へ代謝するS-Adenosyl methionine回路，(II) Hcyからシスタチオニン (Cth)，さらにCysへと代謝する経路により構成されると考えられる．(II) の経路の解明を目的として，CthからCysへと代謝するCystathionine-γ-lyase (CGL), HcyからCthへと代謝するCystathionine-β-synthase (CBS) の機能を有する酵素遺伝子について，105-A株のゲノム配列からの同定を進めてきた．そこで本研究では，それらの機能について，遺伝子欠損株を用いた解析を行った．
  CGLおよびCBSの候補遺伝子について，二重相同組み換え法を用いて遺伝子欠損株を構築した（ΔBL105A_0509，ΔBL105A_0510）．また，ビフィズス菌最少培地 (BMM)に単一有機硫黄源としてCys / Met / Cthを添加した培地を用いた生育試験を行い，野生株とΔBL105A_0509，ΔBL105A_0510の間で生育を比較した．
  BMM+Cys培地ではどちらの欠損株も野生株と同様の生育が観察されたが，BMM+Met培地では両変異株ともに生育の低下が観察された．さらに，BMM+Cth培地ではΔBL105A_0509は野生株と同様に生育したが，ΔBL105A_0510は生育の低下が観察された．これらの結果から，BL105A_0509とBL105A_0510はそれぞれCBS, CGLの機能を持つ遺伝子であると考えられた．
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Setsunan University, 20K05801
• Analysis of the disruption and recovery mechanism on gastrointestinal homeostasis by skatole produced by high animal protein intake
  Grants-in-Aid for Scientific Research
  01 Apr. 2018 - 31 Mar. 2021
  SHIMIZU Hidehisa
  In recent years, increased intake of red and processed meat in particular has been considered to be one of the factors in the development and progression of liver diseases (fatty liver, liver cancer, etc.) and intestinal diseases (inflammatory bowel diseases, colon cancer, etc.). In the present study, we focused on the tryptophan-derived skatole, an intestinal microbiota metabolite, which is produced in the intestine depending on the amount of protein ingested. While skatole leads to dysfunction of the liver and colon, its precursor, indole-3-acetic acid, was suggested to have the opposite effects. Therefore, the results of the present study indicate that skatole and its precursor indole-3-acetic acid may be involved in the cause of the conflicting effects of increased protein intake on health promotion, and development and progression of diseases that have been reported so far.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Shimane University, 18H03178
• Identification and functional analysis of genes important for the activity of bifidobacteria in the intestine harboring intestinal microbiota
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
  01 Apr. 2016 - 31 Mar. 2019
  Fukiya Satoru
  Bifidobacteria are well-known for their beneficial effects on human health, but the molecular mechanisms how bifidobacteria are colonizing and surviving in the intestine are not fully understood especially in the presence of the intestinal microbiota. To clarify the molecular mechanisms, the breeding system of the conventional mice that allows long-term colonization of the human-derived bifidobacteria was established. Using this system, bifidobacterial genes specifically expressed in the intestine were identified by R-IVET analysis. Furthermore, a transposon mutant library comprising 48,000 mutants was constructed and administered to the germ-free mice. Increase and decrease of each mutant in the intestine were comprehensively evaluated by the INSeq analysis. Finally, 442 genes were identified that were important to the colonization and survival in the intestine.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 16H04893
• 腸内細菌叢存在下でのビフィズス菌の腸内活動に重要な遺伝子の同定と機能検証
  科学研究費補助金(基盤研究(B))
  Apr. 2016 - Mar. 2019
  吹谷 智
  文部科学省, Principal investigator, Competitive research funding
• Influence of intestinal fermentation products caused by excessive animal protein intake on intestinal and various organs.
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
  01 Apr. 2015 - 31 Mar. 2018
  Shimizu Hidehisa; MIYAZAKI Hitoshi; ISHIZUKA Satoshi
  In the present study, we analyzed the influence of skatole, an intestinal bacterial metabolite, on rats, cultured intestinal cells, and cultured hepatoma cells. As results, in rats, it was revealed that skatole induces disturbance of bile acid metabolism, change of gene expression in the liver and ileum, and dysbiosis. In the analysis using cultured intestinal cells, it was demonstrated that skatole leads to cell death via AhR activation. Furthermore, in cultured hepatoma cells, it was suggested that skatole promotes cell proliferation through activation of ERK. Based on the above results of the present study, it was shown that the production of skatole in the intestine induces abnormalities in gastrointestinal function, and may also be involved in the onset and progression of gastrointestinal diseases.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Shimane University, 15H03090
• Characterization of bifidobacterial glycosidase: a new colonization factor that promotes bacterial adhesion to the intestinal mucin
  Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up
  28 Aug. 2015 - 31 Mar. 2017
  Nishiyama Keita; Okada Nobuhiko; Mukai Takao; Yamamoto Yuji; Yokota Atsushi; Fukiya Satoru; Urashima Tadasu
  Bifidobacterium is a natural inhabitant of the human gastrointestinal tract. I studied the role of sialidase (SiaBb2) from Bifidobacterium bifidum in gut colonization and carbohydrate catabolism. SiaBb2 cleaves sialyl-human milk oligosaccharides to produce usable oligosaccharides, thus supporting B. bifidum growth. Moreover, SiaBb2 promotes B. bifidum adhesion to mucosal surfaces. This study provides new insights into the role of B. bifidum sialidase as a bifunctional extracellular enzyme that is crucially important for B. bifidum colonization of the gut.
  Japan Society for the Promotion of Science, Grant-in-Aid for Research Activity Start-up, Kitasato University, 15H06581
• Novel mechanism analysis of microcystin on organ dysfunction-focusing on bile acid metabolism-
  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
  01 Apr. 2015 - 31 Mar. 2017
  Shimizu Hidehisa; SUGIURA NORIO; MIYAZAKI HITOSHI; ISHIZUKA SATOSHI; FUKITANI SATORU
  Eutrophication of lake and reservoir accelerates development of Microcystis which produces a toxin, called microcystin. The present study shows that in a cultured hepatocyte cell line, HepG2 cells, AMPK was time- and dose-dependently activated by microcystin-LR (MC-LR) stimulation. When AMPK was not activated in MC-LR-treated HepG2 cells, cell death was induced. However, when MC-LR activated AMPK, cell death was inhibited. In a cultured intestinal cell line, Caco-2 cells, MC-LR leaded to cell proliferation through p38 and JNK activation although ERK activation was not participate. Furthermore, the expression levels of oncogene was upregulated in MC-LR-treated Caco-2 cells. In rat analysis, food intake, body weight, and weight of liver, kidney, and adipose tissue were not difference between control and MC-LR-administrated rats. We will continue to check for serum parameter (ALT/AST, TG, and TC etc), liver TG and TC, and gut microbiota, etc.
  Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Shimane University, 15K12352
• Exploration of bifidobacterial genes involved in the intestinal survival through a dynamics analysis of random transposon mutants of Bifidobacterium longum
  Grants-in-Aid for Scientific Research
  Apr. 2013 - Mar. 2016
  FUKIYA Satoru; OGURA Yoshitoshi
  Bifidobacteria, which exert health-promoting effects, are one of the beneficial members of the human intestinal microbiota. Molecular mechanisms of their intestinal survival are still unclear due to the insufficient development of gene-mutagenesis systems in this genus. This study aimed to identify bifidobacterial genes that contribute to the intestinal survival using combined analysis of a transposon mutagenesis and next-generation sequencing.
  In this study, the complete genomic sequence of the mutagenesis-host strain, Bifidobacterium longum 105-A, was determined. Two different transposon mutagenesis systems were developed using transposable elements ISBlo11, derived from B. longum, and Himar1C9. This progress will pave the way to clarify the molecular mechanisms of bifidobacterial survival in the intestine.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Principal investigator, Competitive research funding, 25450090
• Screening of bifidobacterial genes specifically expressed in the mouse intestine using a site-specific recombination system
  Grants-in-Aid for Scientific Research
  Apr. 2011 - Mar. 2013
  FUKIYA Satoru
  Bifidobacteria are a major component of the human intestinal microbiota and well-known for their health-promoting effects. However, molecular mechanisms of the effects are still unclear. This study aimed to clarify the mechanisms of the effects by indentifying bifidobacterial genes that are specifically expressed in the intestine. A molecular tool for introduction of the foreign genes to bifidobacterial chromosome was developed. Screening system of the specifically-expressed genes has been constructed based on the developed tool. These progresses are thought to be important for providing evidence at a molecular level for the beneficial effects of bifidobacteria.
  Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, Principal investigator, Competitive research funding, 23780072
• 含硫アミノ酸に注目した新規ビフィズス菌増殖因子の開発
  2013 - 2013
  吹谷 智
  本研究ではビフィズス菌の含硫アミノ酸代謝系の解明を目指した.6株のビフィズス菌を用い,含硫アミノ酸の代謝能力を調べた.その結果,メチオニン資化能力の強い株2株,弱い株4株に分類された.メチオニン資化能力が強いBifidobacterium longum 105-A株を対象に,メチオニン資化に重要なC-Sリアーゼの活性を解析した結果,本菌株は 2種類のC-Sリアーゼを持つことと,その1種であるシスタチオニンγ-リアーゼと考えられる酵素がメチオニンにより誘導されることが示唆された.これらの結果は,ビフィズス菌の含硫アミノ酸代謝系の解明に大きく貢献し,新規ビフィズス菌増殖因子の開発につながるものである.
  科学技術振興機構, 産学が連携した研究開発成果の展開/研究成果展開事業/研究成果最適展開支援プログラム(A-STEP)/探索タイプ, 13411379
• Survival strategy of intestinal lactic acid bacteria in the gut : functional analysis of cell surface structure involved in bile acid adaptation
  Grants-in-Aid for Scientific Research(基盤研究(B))
  2009 - 2011
  Atsushi YOKOTA; Satoru FUKIYA; Naoki MORITA; Shin-ichishi YOKOTA
  Free bile acids (FBAs) are considered the most deleterious stress compounds that inhibit the growth of intestinal bacteria by their membrane-damaging effect. Previously, we found adaptation in Lactobacillus gasseri JCM1131T to cholic acid (CA), one of the major FBAs in human intestine. In this adaptation, exponentially growing cells were first exposed to 4 mM CA, a sub-lethal concentration, for 30 min and then to lethal concentration at 15 mM. The adapted cells did not show any appreciable decrease in viability in the presence of 15 mM CA even after 7 h, while non-adapted cells rapidly lost viability by the factors of 10-3~-4. In the adapted cells a significant relief of membrane damage was observed in the presence of CA, suggesting alterations in lipid composition in the cell envelope.Total lipids extracted from adapted and non-adapted cells were separated into three fractions, neutral lipids, glycolipids and phospholipids. Determination of amount of each fraction revealed no significant changes between adapted and non-adapted cells. Likewise, significant differences were neither found in fatty acids compositions of each fraction. However, significant alterations were detected in glycolipids and phospholipids compositions. In glycolipids, increases in the length of sugar chain were detected during the adaptation. Furthermore, a significant increase in relative amount of cardiolipin (CL) was observed in the phospholipid fraction.To evaluate physiological importance of CL in CA adaptation, vesicle experiment was conducted. It was found that CL confers resistance to the liposome against CA attack, suggesting a pivotal role of CL in adaptation mechanism.Mutants having deletion in two putative cardiolipin synthase genes (cls) singly or in combination were then derived, and their abilities in CA adaptation were investigated. Although CL levels of the both single-knockout mutants were lower than that of the wild type JCM1131T, the levels were found to increase after exposure to 4 mM CA, and a comparable CA adaptation to the wild type strain was observed in both the mutants. The double-knockout mutant possessed negligible amount of CL but still showed CA adaptation, suggesting a dispensable role of CL in CA adaptation. However, in this mutant, significant decrease in glycolipid fraction and significant increase in phospholipid fraction were observed as compared to wild-type strain and these shifts were more prominent after CA adaptation. Therefore, CL was found to work as a key determinant for the lipid composition of the bacterial cell membrane.
  Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 21380053
• 転位活性を持つビフィズス菌挿入配列の単離と変異導入系の構築
  科学研究費補助金(若手研究(B))
  2006 - 2007
  吹谷 智
  平成19年度はビフィズス菌からの挿入配列の単離および単離された挿入配列の詳細な解析を行った.1.新規挿入配列様因子TLS16の解析昨年度同定されたBifidobacterium longum BK28株由来の挿入配列様因子TLS16について塩基配列の解析および各種ビフィズス菌における保存性について解析を行った.TLS16は1.59kbpの挿入配列であり,その内部には転位に関与するタンパク質であるトランスポゼースをコードする単一の遺伝子が存在していた.アミノ酸レベルの相同性解析,および因子末端に存在する特徴的なステム・ループ構造などの特徴から,TLS16は挿入配列IS605ファミリーに属する因子と考えられた.またサザンハイブリダイゼーション解析により,TLS16はB.longum,B. breve,B.adolescentis,B.gallicumの基準株にそのホモログが保存されている事を明らかにした.2.転位活性を持つ挿入配列様因子TLS143の解析咋年度構築した転位因子のスクリーニング系を用いて,B.longum 105-A株を用いて転位活性を持つ因子を探索した.その結果,1.43kbpの長さの因子がスクリーニングベクターに挿入されていたため,本因子をTLS143と命名した.TLS143は末端に逆方向反復配列を持つ典型的なIS因子であり,その相同性からからIS3ファミリーに属する因子であると考えられた.1.と同様に各種ビフィズス菌における保存性を調べたところ,TLS16に比べて多くのビフィズス菌種壕存在する普遍性の高い因子であることが明らかになった.ビフィズス菌の転位因子の研究はほとんど行われていなかったが,本研究により二つの因子が同定された.特に,TLS143は転位活性を持つことが証明されたビフィズス菌由来の初めての因子であり,今後更なる応用が期待される.
  文部科学省, 若手研究(B), 北海道大学, Principal investigator, Competitive research funding, 18780050
• Identification and functional analysis of the bile acid transforming intestinal bacteria
  Grant-in-Aid for Scientific Research
  2007
  Competitive research funding
• Screening of novel secondary bile acid-producing intestinal bacteria and clarification of the mechanism of formation of colon-cancer promoter
  Grants-in-Aid for Scientific Research(基盤研究(B))
  2004 - 2006
  Atsushi YOKOTA; 和田 大; Isao YUMOTO; Satoru FUKIYA
  Isolation of novel intestinal bacteria converting primary bile acids such as cholic acid (CA) or chenodeoxycholic acid (CDCA) into colon cancer-promoting secondary bile acids such as deoxycholic acid (DCA) or lithocholic acid (LCA), respectively, was conducted from fecal samples of humans.Total 619 strains were isolated from fecal samples in anaerobic chamber. Their activities for the formation of secondary bile acids including DCA and LCA were examined by culturing them in the medium containing CA or CDCA followed by the detection of the reaction products by TLC, HPLC and GC-MS analyses. As the results, eight strains were obtained as the secondary bile-acid producers. Determination of 16SrRNA gene sequence revealed that two of them producing DCA or LCA were Clostridium scindens and C.leptum, both of which are known producers for DCA or LCA. The other six strains were found to produce 7-oxo-lithocholic acid (3α-hydroxy-7-oxo-5β-cholanoic acid, 7-keto-LCA) from CDCA. These strains consisted of already know producers for 7-keto-LCA including Escherichia coli(three strains) and Bacteroides fragilis (two strains) and one previously not described strain, Bacteroides intestinalis. Therefore, this strain was selected as a novel producer and designated B. intestinalis AM-1. This strain produced 7-keto-DCA from CA. The same activities were also detected in the type strain of B. intestinalis JCM13265^TConversion reaction of strain AM-1 was compared with those of E. coliHB101 and B. fragilis JCM11019^T as the reference strains. It was revealed that strain AM-1 showed conversion yield of more than 90% with lower growth level than the other two strains, resulting in higher activity of conversion per cell than the others. However, we did not observe significant differences in the activities of 7α-hydroxysteroid dehydrogenase, a responsible enzyme producing 7-keto-LCA, in these three strains.
  Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 16380054
• Development of mutagenesis system in bifidobacterial genome
  Grant-in-Aid for Scientific Research
  2006
  Competitive research funding
• Mechanisms for adaptation and torelance toward bile acids in lactic acid bacteria
  2005
  Competitive research funding

• Method for producing demethylated compound
  Patent right, Akira Otani; Hiroaki Yamamoto; Kazuya Mitsuhashi; Takafumi MITACHI; Satoru FUKIYA; Atsushi Yokota, Daicel Corp.
  PCT/JP2022/013830, 24 Mar. 2022
• 新規二次胆汁酸低減剤
  Patent right, 横田 篤; 石塚 敏; 吹谷 智, 国立大学法人北海道大学
  特願2010-048241, 04 Mar. 2010
  特開2011-184311, 22 Sep. 2011
  201103053000544612