Ph. D. (Agriculture), Hokkaido University

Master of Agriculture, Hokkaido University

Applied Microbiology

Plant-Microbe Interactions

Molecular Plant Microbe Interactions

Life Science, Applied microbiology

Environmental Science/Agriculture Science, Plant protection science

Bachelor's degree program, School of Agriculture

Master's degree program, Graduate School of Global Food Resources

Doctoral (PhD) degree program, Graduate School of Global Food Resources

Nov. 2013, 日本農芸化学会北海道支部, 日本農芸化学会北海道支部奨励賞
イネいもち病菌の病原性変異に関する分子遺伝学的研究
曾根 輝雄

Characterization of the redox potential during the fermentation of model wine solutions by Saccharomyces cerevisiae
Kohei Sasaki; Ayumi Abe; Teruo Sone
LETTERS IN APPLIED MICROBIOLOGY, 79, 4, Apr. 2026
English, Scientific journal

Geographical, Seasonal, and Growth-Related Dynamics of Gut Microbiota in a Grapevine Pest, Apolygus spinolae (Heteroptera: Miridae)
Hiroyuki Morimura; Kota Ishigami; Tomoyuki Sato; Teruo Sone; Yoshitomo Kikuchi
Microbial Ecology, 87, 1, Springer Science and Business Media LLC, 09 Sep. 2024
Scientific journal

Rice Blast Field Assessment in Three Regencies Underlies the Importance of Fungicide Resistance Studies in West Java, Indonesia
Hagia Sophia Khairani; Ayumi Abe; Teruo Sone
Jurnal Fitopatologi Indonesia, 20, 4, 165, 173, The Indonesian Phytopathological Society, 29 Jul. 2024, [Peer-reviewed]
Scientific journal, In recent decades, research about rice blast disease in Indonesia has not been focused on the dynamics of the fungus (Pyricularia oryzae) and fungicide use, which resulted in undiscovered pathogen mutations. Observations in Bogor, Cianjur, and Sukabumi Regencies in West Java reveal a high disease incidence (53%–100%), with severity ranging from 8% to 67%. The highest disease severity was recorded in Cikembar District, which is located at the foothill of Mount Gede Pangrango, Sukabumi Regency. Despite a prolonged drought caused by El Niño in 2023, Cikembar still experienced a relatively high disease severity (36%), confirming that this area remains an endemic blast area. The high disease severity, although fungicides were widely used in Cikembar, raises concerns that P. oryzae resistance to fungicides has developed, mainly to isoprothiolane which has been yearly deployed in this area. Farmers are already confronting extra challenges such as unfavorable acidic soil and differences in fungicide use practices decision-making which complicates their control efforts. Laboratory investigations are needed to validate evidence of the emergence of P. oryzae mutations against isoprothiolane in order to provide long-term recommendations for the most effective fungicide use.

The Effect of Hokkaido Red Wines on Vascular Outcomes in Healthy Adult Men: A Pilot Study.
Prae Charoenwoodhipong; Roberta R Holt; Carl L Keen; Nasim Hedayati; Tomoyuki Sato; Teruo Sone; Robert M Hackman
Nutrients, 15, 18, 19 Sep. 2023, [International Magazine]
English, Scientific journal, Moderate red wine intake has been associated with lower cardiovascular mortality, due in part to the intake of polyphenols and anthocyanins, whose content can vary from varietal and year of harvest. This study assessed the vascular effects in response to a single intake of 2015 and 2018 Zweigelt red wines from Hokkaido, Japan. Healthy men were randomly assigned to consume 240 mL each of a red wine, or a sparkling white grape juice as a control in a randomized three-arm cross-over design with a 7 day washout between arms. The augmentation index (AI; a measure of arterial stiffness) and AI at 75 beats/min (AI75), reactive hyperemia index, systolic and diastolic blood pressure (SBP and DBP, respectively), and platelet reactivity were assessed at baseline and two and four hours after each beverage intake. Changes from the baseline were analyzed using a linear mixed model. Significant treatment effects (p = 0.02) were observed, with AI 13% lower after the intake of the 2015 or 2018 vintages compared to the control. Intake of the 2018 vintage reduced SBP and DBP (-4.1 mmHg and -5.6 mmHg, respectively; p = 0.02) compared to the 2015 wine and the control drink. The amount of hydroxytyrosol in the 2018 wine was almost twice the amount as in the 2015 wine, which may help explain the variable blood pressure results. Future studies exploring the vascular effects of the same red wine from different vintage years and different phenolic profiles are warranted.

PANI sensor for monitoring the oxidative degradation of wine using cyclic voltammetry.
Parvin Begum; Liu Yang; Tatsuya Morozumi; Teruo Sone; Toshikazu Kawaguchi
Food chemistry, 414, 135740, 135740, 15 Jul. 2023, [International Magazine]
English, Scientific journal, Redox species in wine are altered by pH and some wines are easily degraded due to oxidation and sulfur dioxide (SO2) reduction. There is a need for quick, easy, simple, and economical methodologies for pH and wine-oxidized products (acetaldehyde) analysis. This study aimed to measure pH and degradation of wines that were electrochemically analyzed using polyaniline (PANI) sensor. Gas chromatography (GC) and fourier transform infrared spectrometer (FT-IR) were also used. Electrochemical analysis showed that oxidation was accelerated and peak currents (Ip,a) and potentials (Ep,a) shifted to negative direction due to acetaldehyde formation. PANI sensor achieved a limit of detection (LOD) of 7 × 10-1 ppm and a sensitivity of 5.20 µA ppm-1 cm-2. Acetaldehyde formation was confirmed by GC (30%) and FT-IR spectra at 1647 cm-1 to the CO vibration of aldehyde. These results suggested that acetaldehyde degraded the taste of wine after remaining open.

Insight into the population dynamics of pathogenic bacteria causing grapevine crown gall in snowfall areas: snow cover protects the proliferation of pathogenic bacteria.
Akira Kawaguchi; Manabu Nemoto; Sunao Ochi; Yosuke Matsushita; Tomoyuki Sato; Teruo Sone
Frontiers in plant science, 14, 1198710, 1198710, Jun. 2023, [International Magazine]
English, Scientific journal, Grapevine crown gall (GCG) is a significant bacterial disease caused by tumorigenic Allorhizobium vitis (TAV) and is prevalent worldwide. TAV infects grapevines through wounds such as freezing injuries. Although grapevines typically avoid being wounded under snow cover, GCG occurs in many commercial vineyards in snowy regions. This study investigated the TAV population in GCG gall tissues, grapevine skins, and snow on grapevine skins from six infected vineyards located in Hokkaido, Japan, an area known for heavy snowfall. TAV was isolated not only from gall tissues but also from skins and snow on skins throughout the year. Hierarchical Bayesian model (HBM) analysis revealed that the number of TAV cells in gall tissues was affected by cultivar and low temperature, while those in skins were affected by location and low temperature. Additionally, Bayesian changepoint detection (BCD) showed that the number of TAV cells in gall and skin tissues increased during winter, including the snowfall season. Furthermore, the TAV population in grapevine skins under the snow was significantly higher than those above the snow, indicating that TAV under the snow is protected by the snow and can survive well during the snowfall season. This study highlights the ability of TAV to overwinter on/in galls and skins under the snow and act as inoculum for the next season.

Prevalence and Characterization of Gentamicin Resistance Genes in Escherichia coli Isolates from Beef Cattle Feces in Japan.
Shiori Yamamoto; Wataru Kitagawa; Motoki Nakano; Hiroshi Asakura; Tatsuya Nakayama; Eriko Iwabuchi; Teruo Sone; Kozo Asano
Current microbiology, 79, 8, 217, 217, 15 Jun. 2022, [International Magazine]
English, Scientific journal, Gentamicin is an important antibiotic for the treatment of opportunistic infections in the clinical field. Gentamicin-resistant bacteria have been detected in livestock animals and can be transmitted to humans through the food supply or direct contact. We have previously revealed that gentamicin-resistant Escherichia coli are distributed at a comparatively high rate from beef cattle in Japan, but few studies have focused on the molecular epidemiology of gentamicin-resistant bacteria. To understand these bacteria, this study examined the prevalence of various gentamicin resistance genes in gentamicin-resistant E. coli isolates from beef cattle feces. Of the 239 gentamicin-resistant E. coli isolates, the presence of the aacC2, aadB, or aac(3)-VIa genes was confirmed in 147, 84, and 8 isolates, respectively. All aac(3)-VIa-harboring isolates had an MIC value of 64 μg/mL for gentamicin and exhibited resistance to 11 antibiotic agents. An analysis of the representative aac(3)-VIa-harboring E. coli strain GC1-3-GR-4 revealed that the aac(3)-VIa gene was present on the IncA/C plasmid together with the aadA and blaCMY genes. Furthermore, the upstream region of the aac(3)-VIa gene contained the aadA gene and the class 1 integron-integrase gene (intI1). The aac(3)-VIa gene was detected for the first time in Japan and is expected to be able to transfer between bacteria via the IncA/C plasmid and integron. These results reveal the expansion of the distribution or diversity of gentamicin resistance genes in Japan.

Origin of Pathogens of Grapevine Crown Gall Disease in Hokkaido in Japan as Characterized by Molecular Epidemiology of Allorhizobium vitis Strains.
Akira Kawaguchi; Teruo Sone; Sunao Ochi; Yosuke Matsushita; Yoshiteru Noutoshi; Mizuho Nita
Life (Basel, Switzerland), 11, 11, 19 Nov. 2021, [International Magazine]
English, Scientific journal, Crown gall is a globally distributed and economically important disease of grapevine and other important crop plants. The causal agent of grapevine crown gall is tumorigenic Allorhizobium vitis (Ti) strains that harbor a tumor-inducing plasmid (pTi). The epidemic of grapevine crown gall has not been widely elucidated. In this study, we investigated the genetic diversity of 89 strains of Ti and nonpathogenic A. vitis to clarify their molecular epidemiology. Multi-locus sequence analysis (MLSA) of the partial nucleotide sequences of pyrG, recA, and rpoD was performed for molecular typing of A. vitis strains isolated from grapevines with crown gall symptoms grown in 30 different vineyards, five different countries, mainly in Japan, and seven genomic groups A to F were obtained. The results of MLSA and logistic regression indicated that the population of genetic group A was significantly related to a range of prefectures and that the epidemic of group A strains originated mainly in Hokkaido in Japan through soil infection. Moreover, group E strains could have been transported by infected nursery stocks. In conclusion, this study indicates that both soil infection and transporting of infected nursery stocks are working as infection source in Hokkaido.

Characterization and proposed spontaneous deletion mechanism of AVR-Pik locus in Pyricularia oryzae.
Phyo Han Thwin; Mai Funabiki; Yuki Tomita; Takehiko Yamazaki; Ayumi Abe; Teruo Sone
Bioscience, biotechnology, and biochemistry, 85, 10, 2217, 2220, 22 Sep. 2021, [International Magazine]
English, Scientific journal, In phytopathogenic fungi, a mutation in the avirulence gene can lead to the breakdown of resistance in the host plant. The nucleotide sequences of the AVR-Pik locus in the strain Ina168 and its spontaneous mutant Ina168m95-5 of Pyricularia oryzae were determined. An AVR-Pik spontaneous deletion mechanism of Ina168m95-5, including multiple homologous recombination events involving repetitive transposable elements, is proposed.

Biodesulfurization of the mixture of dibenzothiophene and its alkylated derivatives by Sphingomonas subarctica T7b
Ida Bagus Wayan Gunam; Teruo Sone; Kozo Asano
Indonesian Journal of Biotechnology, 26, 3, 122, 127, Universitas Gadjah Mada, Research Center for Biotechnology, 2021
English, Scientific journal

Mitochondrial dynamics in Pyricularia oryzae during conidiation monitored by fluorescent microscopy
Dineesha Nipuni Balagalla; Ayumi Abe; Teruo Sone
Journal of General Plant Pathology, Springer Japan, 2021
English, Scientific journal

Function of PoLAE2, a laeA homolog, in appressorium formation and cAMP signal transduction in Pyricularia oryzae
Pradabrat Prajanket; Kim-Chi Thi Vu; Jun Arai; Worawan Sornkom; Ayumi Abe; Teruo Sone
Bioscience, Biotechnology, and Biochemistry, 84, 11, 1, 4, Informa UK Limited, 30 Jul. 2020, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, A novel homolog of laeA, a global regulatory gene in filamentous fungi, was identified from Pyricularia oryzae. A deletion mutant of the homolog (PoLAE2) exhibited lowered intracellular cAMP levels, and decreased appressorium formation on non-host surface; the decrease was recovered using exogenous cAMP and IBMX, indicating that PoLAE2 deletion affected the cAMP signaling pathway.

Three-dimensional visualization of mitochondria in conidia of Pyricularia oryzae using green fluorescent protein (GFP) fused with citrate synthase (CitA)
Muhammad Akhid Syib’li; Aoi Kodama; Ayumi Abe; Teruo Sone
Journal of General Plant Pathology, 86, 4, 250, 256, Springer Science and Business Media LLC, Jul. 2020, [Peer-reviewed], [Corresponding author]
Scientific journal

Double-Stranded Structure of the Polyinosinic-Polycytidylic Acid Molecule to Elicit TLR3 Signaling and Adjuvant Activity in Murine Intranasal A(H1N1)pdm09 Influenza Vaccination
Tetsuo Nakano; Yuki Ohara; Hiroshi Fujita; Akira Ainai; Ei-Tora Yamamura; Tadaki Suzuki; Hideki Hasegawa; Teruo Sone; Kozo Asano
DNA and Cell Biology, 39, 9, 1730, 1740, Mary Ann Liebert Inc, 22 Jun. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Polyinosinic-polycytidylic acid (PIC) is a potent double-stranded RNA (dsRNA) adjuvant useful in intranasal influenza vaccination. In mice, the intensity and duration of immune responses to PIC correlated with the double-stranded chain length. A rational method to avoid PIC chain extension in PIC production is to use multiple short poly(I) molecules and one long poly(C) molecule for PIC assembly. In this study, we elucidate that a newly developed uPIC100-400 molecule comprising multiple 0.1 kb poly(I) molecules and one 0.4 kb poly(C) molecule effectively enhanced the immune responses in mice, by preventing the challenged viral propagation and inducing hemagglutinin-specific IgA, after intranasal A(H1N1)pdm09 influenza vaccination. Reduced intraperitoneal toxicity of PIC prepared with multiple short poly(I) molecules in mice indicates the widened effective range of uPIC100-400 as an adjuvant. In contrast to uPIC100-400, the PIC molecule comprising multiple 0.05 kb poly(I) molecules failed to elicit mouse mucosal immunity. These results were consistent with TLR3 response but not retinoic acid inducible gene I (RIG-I)-like receptor response in the cell assays, which suggests that the adjuvant effect of PIC in mouse intranasal immunization depends on TLR3 signaling. In conclusion, the double-stranded PIC with reduced toxicity developed in this study would contribute to the development of PIC-adjuvanted vaccines.

Plasmid Sequences of Four Large Plasmids Carrying Antimicrobial Resistance Genes in Escherichia coli Strains Isolated from Beef Cattle in Japan.
Shiori Yamamoto; Wataru Kitagawa; Motoki Nakano; Hiroshi Asakura; Eriko Iwabuchi; Teruo Sone; Kozo Asano
Microbiology resource announcements, 9, 20, 14 May 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Escherichia coli is a common reservoir for antimicrobial resistance genes that can be easily transformed to possess multidrug resistance through plasmid transfer. To understand multidrug resistance plasmids, we report the plasmid sequences of four large plasmids carrying a number of genes related to antimicrobial resistance that were found in E. coli strains isolated from beef cattle.

A Peptidoglycan Amidase Mutant of Burkholderia insecticola Adapts an L-form-like Shape in the Gut Symbiotic Organ of the Bean Bug Riptortus pedestris
Shiori Goto; Tsubasa Ohbayashi; Kazutaka Takeshita; Teruo Sone; Yu Matsuura; Peter Mergaert; Yoshitomo Kikuchi
Microbes and Environments, 35, 4, n/a, n/a, Japanese Society of Microbial Ecology, 2020
Scientific journal

A Peptidoglycan Amidase Mutant of Burkholderia insecticola Adapts an L-form-like Shape in the Gut Symbiotic Organ of the Bean Bug Riptortus pedestris
Goto Shiori; Ohbayashi Tsubasa; Takeshita Kazutaka; Sone Teruo; Matsuura Yu; Mergaert Peter; Kikuchi Yoshitomo
Microbes and Environments, 35, 4, n/a, Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles, 2020, [Domestic magazines]
English, Scientific journal,

Bacterial cell shapes may be altered by the cell cycle, nutrient availability, environmental stress, and interactions with other organisms. The bean bug Riptortus pedestris possesses a symbiotic bacterium, Burkholderia insecticola, in its midgut crypts. This symbiont is a typical rod-shaped bacterium under in vitro culture conditions, but changes to a spherical shape inside the gut symbiotic organ of the host insect, suggesting the induction of morphological alterations in B. insecticola by host factors. The present study revealed that a deletion mutant of a peptidoglycan amidase gene (amiC), showing a filamentous chain form in vitro, adapted a swollen L-form-like cell shape in midgut crypts. Spatiotemporal observations of the ΔamiC mutant in midgut crypts revealed the induction of swollen cells, particularly prior to the molting of insects. To elucidate the mechanisms underlying in vivo-specific morphological alterations, the symbiont was cultured under 13 different conditions and its cell shape was examined. Swollen cells, similar to symbiont cells in midgut crypts, were induced when the mutant was treated with fosfomycin, an inhibitor of peptidoglycan precursor biosynthesis. Collectively, these results strongly suggest that the Burkholderia symbiont in midgut crypts is under the control of the host insect via a cell wall-attacking agent.

Comparative cytology, physiology and transcriptomics of Burkholderia insecticola in symbiosis with the bean bug Riptortus pedestris and in culture
Tsubasa Ohbayashi; Ryo Futahashi; Mia Terashima; Quentin Barrière; Florian Lamouche; Kazutaka Takeshita; Xian-Ying Meng; Yasuo Mitani; Teruo Sone; Shuji Shigenobu; Takema Fukatsu; Peter Mergaert; Yoshitomo Kikuchi
The ISME Journal, 13, 6, 1469, 1483, Jun. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal

Conductive Iron Oxides Promote Methanogenic Acetate Degradation by Microbial Communities in a High-Temperature Petroleum Reservoir.
Souichiro Kato; Kaoru Wada; Wataru Kitagawa; Daisuke Mayumi; Masayuki Ikarashi; Teruo Sone; Kozo Asano; Yoichi Kamagata
Microbes and environments, 34, 1, 95, 98, 30 Mar. 2019, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, Supplementation with conductive magnetite particles promoted methanogenic acetate degradation by microbial communities enriched from the production water of a high-temperature petroleum reservoir. A microbial community analysis revealed that Petrothermobacter spp. (phylum Deferribacteres), known as thermophilic Fe(III) reducers, predominated in the magnetite-supplemented enrichment, whereas other types of Fe(III) reducers, such as Thermincola spp. and Thermotoga spp., were dominant under ferrihydrite-reducing conditions. These results suggest that magnetite induced interspecies electron transfer via electric currents through conductive particles between Petrothermobacter spp. and methanogens. This is the first evidence for possible electric syntrophy in high-temperature subsurface environments.

Novel methods for nucleotide length control in double-stranded polyinosinic-polycytidylic acid production using uneven length components
Tetsuo Nakano; Ei-Tora Yamamura; Hiroshi Fujita; Teruo Sone; Kozo Asano
Bioscience, Biotechnology, and Biochemistry, 82, 11, 1889, 1901, Informa UK Limited, 02 Nov. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Polyinosinic-polycytidylic acid (PIC), a double-stranded RNA that induces innate immunity in mammals, is a candidate immunopotentiator for pharmaceuticals. The potency and adverse effects of PIC are strongly correlated with the nucleotide length, and the inability to precisely control the length in PIC production limits its practical use. Length extension during the annealing process is the major factor underlying the lack of control, but tuning the annealing conditions is insufficient to resolve this issue. In this study, we developed a novel method to produce accurate nucleotide length PIC at an industrial scale. The length extension was significantly suppressed by the assembly of multiple short polyinosinic acid molecules with one long polycytidylic acid molecule. A newly developed PIC, uPIC100-400, demonstrated a reproducible length and better storage stability than that of corresponding evenly structured PIC. Human dsRNA receptors exhibited equivalent responsiveness to uPIC100-400 and the evenly structured PIC with the same length.

Isolation of Previously Uncultured Slow-Growing Bacteria by Using a Simple Modification in the Preparation of Agar Media.
Souichiro Kato; Ayasa Yamagishi; Serina Daimon; Kosei Kawasaki; Hideyuki Tamaki; Wataru Kitagawa; Ayumi Abe; Michiko Tanaka; Teruo Sone; Kozo Asano; Yoichi Kamagata
Applied and environmental microbiology, 84, 19, 01 Oct. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Most microorganisms living in the environment have yet to be cultured, owing at least in part to their slow and poor propagation properties and susceptibility to oxidative stress. Our previous studies demonstrated that a simple modification in the preparation of agar media, i.e., autoclaving the phosphate and agar separately (termed "PS" medium), can greatly improve the culturability of microorganisms by mitigating oxidative stress compared with the use of "PT" medium (autoclaving the phosphate and agar together). Here, we attempted to isolate phylogenetically novel bacteria by combining PS medium with prolonged cultivation. After inoculation with forest soil or pond sediment samples, significantly more colonies appeared on PS medium than on PT medium. A total of 98 and 74 colonies that emerged after more than 7 days of cultivation were isolated as slow growers from PS and PT media, respectively. Sequencing analysis of their 16S rRNA genes revealed that the slow growers recovered from PS medium included more phylogenetically novel bacteria than those from PT medium, including a strain that could be classified into a novel order in the class Alphaproteobacteria Further physiological analysis of representative strains showed that they were actually slow and poor growers and formed small but visible colonies only on PS medium. This study demonstrates that the culturability of previously uncultured bacteria can be improved by using an isolation strategy that combines a simple modification in medium preparation with an extended incubation time.IMPORTANCE Most microbial species inhabiting natural environments have not yet been isolated. One of the serious issues preventing their isolation is intrinsically slow and/or poor growth. Moreover, these slow and/or poor growers are likely to be highly sensitive to environmental stresses, especially oxidative stress. We reported previously that interaction between agar and phosphate during autoclave sterilization generates hydrogen peroxide, which adversely affects the culturability of environmental microorganisms, in particular, slow-growing organisms vulnerable to oxidative stress. In this study, we successfully isolated many slow-growing bacterial strains with phylogenetic novelty by simply modifying their cultivation on agar plates, i.e., autoclaving the phosphate and agar separately. The current limited repertoire of culture techniques still has room for improvement in the isolation of microorganisms previously considered unculturable.

Burkholderia insecticola sp nov., a gut symbiotic bacterium of the bean bug Riptortus pedestris
Takeshita Kazutaka; Tamaki Hideyuki; Ohbayashi Tsubasa; Meng Xian-Ying; Sone Teruo; Mitani Yasuo; Peeters Charlotte; Kikuchi Yoshitomo; Vandamme Peter
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 68, 7, 2370, 2374, Jul. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal

The DNA damage signal transducer ortholog Mop53BP1 is required for proper appressorium differentiation and pathogenicity in Pyricularia oryzae
Andre Ohara; Yui Tashika; Ayumi Abe; Teruo Sone
Journal of General Plant Pathology, 84, 3, 176, 188, Springer Tokyo, 01 May 2018, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Fluorescent reporter analysis revealed the timing and localization of AVR-Pia expression, an avirulence effector of Magnaporthe oryzae
Worawan Sornkom; Shinsuke Miki; Saori Takeuchi; Ayumi Abe; Kozo Asano; Teruo Sone
MOLECULAR PLANT PATHOLOGY, 18, 8, 1138, 1149, Oct. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Diversity of lactic acid bacteria from Miang, a traditional fermented tea leaf in northern Thailand and their tannin-tolerant ability in tea extract
Siriporn Chaikaew; Sasitorn Baipong; Teruo Sone; Apinun Kanpiengjai; Naradorn Chui-chai; Kozo Asano; Chartchai Khanongnuch
JOURNAL OF MICROBIOLOGY, 55, 9, 720, 729, Sep. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Restoration of the growth of Escherichia coli under K+-deficient conditions by Cs+ incorporation via the K+ transporter Kup.
Souichiro Kato; Yoshiki Kanata; Wataru Kitagawa; Teruo Sone; Kozo Asano; Yoichi Kamagata
Scientific reports, 7, 1, 1965, 1965, 16 May 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal

Burkholderia of plant-beneficial group are symbiotically associated with bordered plant bugs (Heteroptera: Pyrrhocoroidea: Largidae)
Kazutaka Takeshita; Yu Matsuura; Hideomi Itoh; Ronald Navarro; Tomoyuki Hori; Teruo Sone; Yoichi Kamagata; Peter Mergaert; Yoshitomo Kikuchi
Microbes and Environments, 30, 4, 321, 329, 22 Dec. 2015, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Solution structure of an avirulence protein, AVR-Pia, from Magnaporthe oryzae
Toyoyuki Ose; Azusa Oikawa; Yukiko Nakamura; Katsumi Maenaka; Yuya Higuchi; Yuki Satoh; Shiho Fujiwara; Makoto Demura; Teruo Sone; Masakatsu Kamiya
JOURNAL OF BIOMOLECULAR NMR, 63, 2, 229, 235, Oct. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal

Use of native promoter-eGFP as a gene reporter on onion epidermis to analyze gene expression of AVR-Pia, an avirulence effector of rice blast pathogen
Worawan Sornkom; Kozo Asano; Teruo Sone
Engineering Journal, 19, 3, 85, 93, Chulalongkorn University, 05 Jun. 2015, [Peer-reviewed]
English, Scientific journal

Structural characterization reveals the keratinolytic activity of an arthrobacter nicotinovorans protease
Teruo Sone; Yumiko Haraguchi; Aki Kuwahara; Toyoyuki Ose; Megumi Takano; Ayumi Abe; Michiko Tanaka; Isao Tanaka; Kozo Asano
Protein and Peptide Letters, 22, 1, 63, 72, Bentham Science Publishers B.V., 2015, [Peer-reviewed]
English, Scientific journal

Characterization of Multi-antibiotic-resistant Escherichia coli Isolated from Beef Cattle in Japan
Shiori Yamamoto; Motoki Nakano; Wataru Kitagawa; Michiko Tanaka; Teruo Sone; Katsuya Hirai; Kozo Asano
MICROBES AND ENVIRONMENTS, 29, 2, 136, 144, Jul. 2014, [Peer-reviewed]
English, Scientific journal

Heterologous production, purification, and immunodetection of Magnaporthe oryzae avirulence protein AVR-Pia
Yuki Satoh; Shinsuke Miki; Toyoyuki Ose; Azusa Oikawa; Katsumi Maenaka; Ryouhei Terauchi; Kozo Asano; Teruo Sone
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 78, 4, 680, 686, Apr. 2014, [Peer-reviewed]
English, Scientific journal

Nonomuraea sp ID06-A0189 inulin fructotransferase (DFA III-forming): gene cloning, characterization and conservation among other Nonomuraea species
Sri Pudjiraharti; Midori Ohtani; Nanami Takano; Ayumi Abe; Puspita Lisdiyanti; Michiko Tanaka; Teruo Sone; Kozo Asano
JOURNAL OF ANTIBIOTICS, 67, 2, 137, 141, Feb. 2014, [Peer-reviewed]
English, Scientific journal

Draft genome sequence of Tomitella biformata AHU 1821T, isolated from a permafrost ice wedge in Alaska
Kentaro Funo; Wataru Kitagawa; Michiko Tanaka; Teruo Sone; Kozo Asano; Yoichi Kamagata
Genome Announcements, 2, 1, American Society for Microbiology, 2014, [Peer-reviewed]
English, Scientific journal

Draft genome sequence of methanol-utilizing Methylophilus sp. strain OH31, isolated from pond sediment in Hokkaido, Japan
Takahiro Kugo; Wataru Kitagawa; Yoshinori Shimomura; Takuya Yamagishi; Michiko Tanaka; Teruo Sone; Kozo Asano; Yoichi Kamagata
Genome Announcements, 2, 2, American Society for Microbiology, 2014, [Peer-reviewed]
English, Scientific journal

Raffinose production using alpha-galactosidase from Paraphaeosphaeria sp.
Hiroaki Nakata; Hiroaki Sakurai; Taizo Nagura; Michiko Tanaka; Teruo Sone; Kozo Asano
MYCOSCIENCE, 54, 4, 247, 251, Jul. 2013, [Peer-reviewed]
English, Scientific journal

Biodesulfurization of dibenzothiophene and its derivatives using resting and immobilized cells of Sphingomonas subarctica T7b.
Ida Bagus Wayan Gunam; Kenta Yamamura; I Nengah Sujaya; Nyoman Semadi Antara; Wayan Redi Aryanta; Michiko Tanaka; Fusao Tomita; Teruo Sone; Kozo Asano
Journal of microbiology and biotechnology, 23, 4, 473, 82, Apr. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal

Homologous recombination causes the spontaneous deletion of AVR-Pia in Magnaporthe oryzae.
Teruo Sone; Saori Takeuchi; Shinsuke Miki; Yuki Satoh; Keisuke Ohtsuka; Ayumi Abe; Kozo Asano
FEMS microbiology letters, 339, 2, 102, 9, Feb. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal, AVR-Pia, an avirulence gene in the genome of the rice blast fungus Magnaporthe oryzae, triggers a hypersensitive reaction in rice cultivars harbouring the resistance gene Pia. The copy number of AVR-Pia was revealed to vary from one to three among M. oryzae isolates avirulent to Pia rice, and three copies of the gene were located on a single chromosome in strain Ina168, from which the gene was originally cloned. The spontaneous avr-Pia mutant originated from Ina168, named Ina168m95-1, which lacks the AVR-Pia gene, and was therefore used to elucidate the molecular mechanism of the deletion of all three copies of AVR-Pia. Screening and analysis of cosmid clones indicated that two copies of the DNA-type transposon Occan (Occan(9E12) and Occan(3A3) ) were located on the same chromosome, and three copies of AVR-Pia were located in between the two Occan elements. Ina168m95-1 contains a conserved Occan element, named Occan(m95-1) , between sequences homologous to the 5'-flanking region of Occan(3A3) and the 3'-flanking region of Occan(9E12) . In addition, sequence polymorphisms indicated a homologous recombination between Occan(3A3) and Occan(9E12) , which resulted in Occan(m95-1) . Based on these observations, we propose the hypothesis that homologous recombination in the two Occan elements leads to the deletion of AVR-Pia in Ina168m95-1.

Comparison of Yacon (Smallanthus sonchifolius) Tuber with Commercialized Fructo-oligosaccharides (FOS) in Terms of Physiology, Fermentation Products and Intestinal Microbial Communities in Rats.
Ni Wayan Arya Utami; Teruo Sone; Michiko Tanaka; Cindy H Nakatsu; Akihiko Saito; Kozo Asano
Bioscience of microbiota, food and health, 32, 4, 167, 78, 2013, [Peer-reviewed], [Domestic magazines]
English, Scientific journal, The yacon (Smallanthus sonchifolius) tuber was examined with regard to its prebiotic effects compared with commercialized fructo-oligosaccharides (FOS). A feed containing 10% yacon tuber, which is equivalent to 5% commercialized FOS in terms of the amount of fructo-oligosaccharides (GF2, GF3 and GF4), was administrated to rats for 28 days. The yacon diet changed the intestinal microbial communities beginning in the first week, resulting in a twofold greater concentration of cecal short-chain fatty acids (SCFAs). The SCFA composition differed, but the cecal pH in rats fed yacon tuber was equal to that in rats fed FOS. Serum triglycerides were lower in rats fed yacon compared with rats fed FOS and the control diet. Cecal size was greater with the yacon tuber diet compared with the control diet. The abundant fermentation in the intestines created a selective environment for the intestinal microbiota, which included Lactobacillus acidophilus, Bifidobacterium pseudolongum, Bifidobacterium animalis and Barnesiella spp. according to identification with culture-independent analysis, 16S rRNA gene PCR-DGGE combined with cloning and sequencing. Barnesiella spp. and B. pseudolongum were only found in the rats fed the yacon diet, while L. acidophilus and B. animalis were found in abundance in rats fed both the yacon and FOS diets. The genus Barnesiella has not previously been reported to be associated with yacon or FOS fermentation. We concluded that the physiological and microbiological effects of the yacon tuber were different from those of FOS. Differences in cecal size, blood triglycerides and microbial community profiles including their metabolites (SCFAs) between the yacon tuber and FOS were shown to be more greatly affected by the yacon tuber rather than FOS.

Localization of resistance genes on the multiple-drug resistance plasmids in Escherichia coli isolated from beef cattle
Yamamoto S; Nakano M; Tanaka M; Kitagawa W; Sone T; Asano K
International Journal of Antimicrobial Agents, 42, S84, 2013, [Peer-reviewed]

Purification and characterization of phytase from Klebsiella pneumoniae 9-3B.
Lotis Escobin-Mopera; Midori Ohtani; Sachie Sekiguchi; Teruo Sone; Ayumi Abe; Michiko Tanaka; Vithaya Meevootisom; Kozo Asano
Journal of bioscience and bioengineering, 113, 5, 562, 7, May 2012, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

O-405 Screening of novel enzyme for effective saccharification of alkali-treated rice straw
Iwai Takao; Ito Yumi; Kudo Ayako; Tanaka Michiko; Abe Ayumi; Sone Teruo; Shiroma Riki; Tokuyasu Ken; Yabe Kimiko; Nagashima Minoru; Asano Kozo
Proceedings of the Conference on Biomass Science, 7, 46, 47, The Japan Institute of Energy, 2012
Japanese, Rice straw is a new resource of second-generation bioethanol. We estimated that endophyte (microorganisms living in plant without sympton) secrete cell wall degrading enzyme when invade into host plant and they may be useful enzyme resources. Result of screening of endophytes, extracellular enzyme from 4 strains increased xylan saccharification. Especially, enzymes cocktail of commercial enzymes and enzymes from Fusarium sp. E12-4CA can degrade xylan more than 90%. Crude enzymes from 4 endophytes indicated high hemicellulase activities, so the possibility of synergistic saccharification of commercial enzymes and the crude enzymes.

Characterization of Inago1 and Inago2 retrotransposons in Magnaporthe oryzae
Edmundo Sanchez; Kozo Asano; Teruo Sone
JOURNAL OF GENERAL PLANT PATHOLOGY, 77, 4, 239, 242, Jul. 2011
English, Scientific journal

Actinomycete Nonomuraea sp. isolated from Indonesian soil is a new producer of inulin fructotransferase.
Sri Pudjiraharti; Nobuchika Takesue; Taiki Katayama; Puspita Lisdiyanti; Muhamad Hanafi; Michiko Tanaka; Teruo Sone; Kozo Asano
Journal of bioscience and bioengineering, 111, 6, 671, 4, Jun. 2011, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes.
Yudai Okuyama; Hiroyuki Kanzaki; Akira Abe; Kentaro Yoshida; Muluneh Tamiru; Hiromasa Saitoh; Takahiro Fujibe; Hideo Matsumura; Matt Shenton; Dominique Clark Galam; Jerwin Undan; Akiko Ito; Teruo Sone; Ryohei Terauchi
The Plant journal : for cell and molecular biology, 66, 3, 467, 79, May 2011, [Peer-reviewed], [International Magazine]
English, Scientific journal

EGFP-Rhm51 foci enable the visualization and enumeration of DNA double-strand breaks in Magnaporthe oryzae
Sali Atanga Ndindeng; Shinsuke Miki; Ayumi Abe; Kozo Asano; Teruo Sone
JOURNAL OF GENERAL PLANT PATHOLOGY, 76, 6, 377, 381, Dec. 2010
English, Scientific journal

Tomitella biformata gen. nov., sp. nov., a new member of the suborder Corynebacterineae isolated from a permafrost ice wedge.
Taiki Katayama; Tomoko Kato; Michiko Tanaka; Thomas A Douglas; Anatoli Brouchkov; Ayumi Abe; Teruo Sone; Masami Fukuda; Kozo Asano
International journal of systematic and evolutionary microbiology, 60, Pt 12, 2803, 7, Dec. 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal

One of two major paralogs of AVR-Pita1 is functional in Japanese rice blast isolates.
Mami Takahashi; Taketo Ashizawa; Kazuyuki Hirayae; Jouji Moriwaki; Teruo Sone; Ryoichi Sonoda; Masako Tsujimoto Noguchi; Susumu Nagashima; Kouji Ishikawa; Michiyoshi Arai
Phytopathology, 100, 6, 612, 8, Jun. 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal

Enzymatic synthesis of Mycoplasma fermentans specific glycoglycerophospholipid from 1,2-dipalmitoylglycerol.
Noriko Ishida; Daisuke Irikura; Kazuhiro Matsuda; Seiji Sato; Teruo Sone; Michiko Tanaka; Kozo Asano
Journal of bioscience and bioengineering, 109, 4, 341, 5, Apr. 2010, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

A molecular phylogeny-based taxonomy of the genus Rhizopus.
Ayumi Abe; Kozo Asano; Teruo Sone
Bioscience, biotechnology, and biochemistry, 74, 7, 1325, 31, 2010, [Peer-reviewed], [International Magazine]
English, Scientific journal

A real-time PCR method targeting a gene sequence encoding 16S rRNA processing protein, rimM, for detection and enumeration of Streptococcus thermophilus in dairy products
Martin Patrick Ongol; Michiko Tanaka; Teruo Sone; Kozo Asano
FOOD RESEARCH INTERNATIONAL, 42, 8, 893, 898, Oct. 2009
English, Scientific journal

Identification and characterization of Rhizot, a novel LTR retrotransposon of Rhizopus oryzae and R. delemar.
Ayumi Abe; Kozo Asano; Teruo Sone
Bioscience, biotechnology, and biochemistry, 73, 8, 1860, 2, Aug. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal

Genomic analysis of the basal lineage fungus Rhizopus oryzae reveals a whole-genome duplication.
Li-Jun Ma; Ashraf S Ibrahim; Christopher Skory; Manfred G Grabherr; Gertraud Burger; Margi Butler; Marek Elias; Alexander Idnurm; B Franz Lang; Teruo Sone; Ayumi Abe; Sarah E Calvo; Luis M Corrochano; Reinhard Engels; Jianmin Fu; Wilhelm Hansberg; Jung-Mi Kim; Chinnappa D Kodira; Michael J Koehrsen; Bo Liu; Diego Miranda-Saavedra; Sinead O'Leary; Lucila Ortiz-Castellanos; Russell Poulter; Julio Rodriguez-Romero; José Ruiz-Herrera; Yao-Qing Shen; Qiandong Zeng; James Galagan; Bruce W Birren; Christina A Cuomo; Brian L Wickes
PLoS genetics, 5, 7, e1000549, Jul. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal

Biotransformation of daidzein to equol by crude enzyme from Asaccharobacter celatus AHU1763 required an anaerobic environment.
Charin Thawornkuno; Michiko Tanaka; Teruo Sone; Kozo Asano
Bioscience, biotechnology, and biochemistry, 73, 6, 1435, 8, Jun. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal

Molecular cloning and expression of a novel cholinephosphotransferase involved in glycoglycerophospholipid biosynthesis of Mycoplasma fermentans.
Noriko Ishida; Daisuke Irikura; Kazuhiro Matsuda; Seiji Sato; Teruo Sone; Michiko Tanaka; Kozo Asano
Current microbiology, 58, 6, 535, 40, Jun. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal

Effect of an additionally introduced degQ gene on di-D-fructofuranosyl 2,6':2',6 anhydride (DFA IV) production by recombinant Bacillus subtilis in a single culture production system.
Nobuchika Takesue; Teruo Sone; Michiko Tanaka; Fusao Tomita; Kozo Asano
Journal of bioscience and bioengineering, 107, 6, 623, 9, Jun. 2009, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Molecular cloning and characterization of the AVR-Pia locus from a Japanese field isolate of Magnaporthe oryzae.
Shinsuke Miki; Kotaro Matsui; Hideki Kito; Keisuke Otsuka; Taketo Ashizawa; Nobuko Yasuda; Satoru Fukiya; Junko Sato; Kazuyuki Hirayae; Yoshikatsu Fujita; Toshihiko Nakajima; Fusao Tomita; Teruo Sone
Molecular plant pathology, 10, 3, 361, 74, May 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal

Molecular characterization of the relationships among Amylomyces rouxii, Rhizopus oryzae, and Rhizopus delemar.
Hideki Kito; Ayumi Abe; I-Nengah Sujaya; Yuji Oda; Kozo Asano; Teruo Sone
Bioscience, biotechnology, and biochemistry, 73, 4, 861, 4, 23 Apr. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal

Potential of selected strains of lactic acid bacteria to induce a Th1 immune profile.
Martin Patrick Ongol; Takeshi Iguchi; Michiko Tanaka; Teruo Sone; Hiroaki Ikeda; Kozo Asano; Takashi Nishimura
Bioscience, biotechnology, and biochemistry, 72, 11, 2847, 57, Nov. 2008, [Peer-reviewed], [International Magazine]
English, Scientific journal

1Dp18 Production of soybean bioactive peptides by recombinant E. coli.
KATO Rika; SHIRATORI Yuko; SONE Teruo; HIRA Tohru; HARA Hiroshi; ASANO Kozo
日本生物工学会大会講演要旨集, 20, 0, 117, 117, The Society for Biotechnology, Japan, 11 Jul. 2008
Japanese

Asaccharobacter celatus gen. nov., sp. nov., isolated from rat caecum.
Kimiko Minamida; Kyohei Ota; Megumi Nishimukai; Michiko Tanaka; Ayumi Abe; Teruo Sone; Fusao Tomita; Hiroshi Hara; Kozo Asano
International journal of systematic and evolutionary microbiology, 58, Pt 5, 1238, 40, May 2008, [Peer-reviewed], [International Magazine]
English, Scientific journal, An obligately anaerobic and equol-producing bacterium, designated strain do03T, was isolated from the caecal content of a rat. Cells were Gram-positive, non-spore-forming rods. The results from a phylogenetic analysis based on 16S rRNA gene sequences showed that strain do03T formed a separate line of descent in the phylogenetic cluster of the family Coriobacteriaceae. The strain was unable to metabolize glucose or other carbohydrates as sole carbon sources; growth was enhanced in the presence of arginine. The cell wall contained meso-diaminopimelic acid. The major fatty acid was C18 : 1cis9 (54.0 %). The strain had one unidentified predominant (91.9 %) quinone that was not menaquinone, methylmenaquinone, demethylmenaquinone, ubiquinone or rhodoquinone. The DNA G+C content was 63 mol%. The data presented in this work show that strain do03T differs from members of the related recognized genera Eggerthella and Denitrobacterium at both the phylogenetic and phenotypic level. Therefore, the strain constitutes a novel genus and species, for which the name Asaccharobacter celatus gen. nov., sp. nov. is proposed. The type strain of the type species is do03T (=JCM 14811T=DSM 18785T=AHU 1763T).

Production of di-D-fructofranosyl-2,6 ': 2 ',6-anhydride (DFA IV) by recombinant Bacillus subtilis carrying heterogenous levan fructotransferase from Arthrobacter nicotinovorans GS-9
Nobuchika Takesue; Teruo Sone; Michiko Tanaka; Fusao Tomita; Kozo Asano
ENZYME AND MICROBIAL TECHNOLOGY, 41, 6-7, 673, 676, Nov. 2007
English, Scientific journal

Rhizopus delemar is the proper name for Rhizopus oryzae fumaric-malic acid producers
Ayumi Abe; Yuji Oda; Kozo Asano; Teruo Sone
MYCOLOGIA, 99, 5, 714, 722, Sep. 2007
English, Scientific journal

Yoghurt fermented by Lactobacillus delbrueckii subsp. bulgaricus H+ -ATPase-defective mutants exhibits enhanced viability of Bifidobacterium breve during storage.
Martin Patrick Ongol; Yuki Sawatari; Yoshiko Ebina; Teruo Sone; Michiko Tanaka; Fusao Tomita; Atsushi Yokota; Kozo Asano
International journal of food microbiology, 116, 3, 358, 66, 30 May 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal

Determination of enzymes from Colletotrichum sp. AHU9748 essential for lepidimoide production from okra polysaccharide.
Chayaporn Saranpuetti; Michiko Tanaka; Teruo Sone; Kozo Asano; Fusao Tomita
Journal of bioscience and bioengineering, 102, 5, 452, 6, Nov. 2006, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

The molecular phylogeny of the genus Rhizopus based on rDNA sequences.
Ayumi Abe; Yuji Oda; Kozo Asano; Teruo Sone
Bioscience, biotechnology, and biochemistry, 70, 10, 2387, 93, Oct. 2006, [Peer-reviewed], [International Magazine]
English, Scientific journal

Production of equol from daidzein by gram-positive rod-shaped bacterium isolated from rat intestine.
Kimiko Minamida; Michiko Tanaka; Ayumi Abe; Teruo Sone; Fusao Tomita; Hiroshi Hara; Kozo Asano
Journal of bioscience and bioengineering, 102, 3, 247, 50, Sep. 2006, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Biodesulfurization of alkylated forms of dibenzothiophene and benzothiophene by Sphingomonas subarctica T7b.
Ida Bagus Wayan Gunam; Yosuke Yaku; Makoto Hirano; Kenta Yamamura; Fusao Tomita; Teruo Sone; Kozo Asano
Journal of bioscience and bioengineering, 101, 4, 322, 7, Apr. 2006, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Construction of pDESTR, a GATEWAY vector for gene disruption in filamentous fungi.
Ayumi Abe; Evelyn B Elegado; Teruo Sone
Current microbiology, 52, 3, 210, 5, Mar. 2006, [Peer-reviewed], [International Magazine]
English, Scientific journal

Effects of long-term ingestion of difructose anhydride III (DFA III) on intestinal bacteria and bile acid metabolism in humans.
Kimiko Minamida; Chikako Asakawa; I Nengah Sujaya; Maki Kaneko; Ayumi Abe; Teruo Sone; Hiroshi Hara; Kozo Asano; Fusao Tomita
Journal of bioscience and bioengineering, 101, 2, 149, 56, Feb. 2006, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Oxidative stress induces high transcription of Rhm52 and Rhm54, novel genes identified as being involved in recombinational repair in Magnaporthe grisea
Evelyn Elegado; Asumi Iwasaki; Marites Sales; Chizu Ishii; Fusao Tomita; Teruo Sone
Journal of General Plant Pathology, 72, 1, 16, 19, Feb. 2006
English, Scientific journal

Development and assessment of a real-time pcr assay for rapid and sensitive detection of a novel thermotolerant bacterium, Lactobacillus thermotolerans, in chicken feces.
Abu Sadeque Md Selim; Piyanuch Boonkumklao; Teruo Sone; Apinya Assavanig; Masaru Wada; Atsushi Yokota
Applied and environmental microbiology, 71, 8, 4214, 9, Aug. 2005, [Peer-reviewed], [International Magazine]
English, Scientific journal

Effects of difructose anhydride III (DFA III) administration on bile acids and growth of DFA III-assimilating bacterium Ruminococcus productus on rat intestine.
Kimiko Minamida; Maki Kaneko; Midori Ohashi; I Nengah Sujaya; Teruo Sone; Masaru Wada; Atsushi Yokota; Hiroshi Hara; Kozo Asano; Fusao Tomita
Journal of bioscience and bioengineering, 99, 6, 548, 54, Jun. 2005, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Effects of difructose anhydride III (DFA III) administration on rat intestinal microbiota.
Kimiko Minamida; Kazuki Shiga; I Nengah Sujaya; Teruo Sone; Atsushi Yokota; Hiroshi Hara; Kozo Asano; Fusao Tomita
Journal of bioscience and bioengineering, 99, 3, 230, 6, Mar. 2005, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

Scalusamides A-C, new pyrrolidine alkaloids from the marine-derived fungus Penicillium citrinum.
Masashi Tsuda; Mai Sasaki; Takao Mugishima; Kazusei Komatsu; Teruo Sone; Michiko Tanaka; Yuzuru Mikami; Jun'ichi Kobayashi
Journal of natural products, 68, 2, 273, 6, Feb. 2005, [Peer-reviewed], [International Magazine]
English, Scientific journal

Citrinadin A, a novel pentacyclic alkaloid from marine-derived fungus Penicillium citrinum.
Masashi Tsuda; Yuu Kasai; Kazusei Komatsu; Teruo Sone; Michiko Tanaka; Yuzuru Mikami; Jun'ichi Kobayashi
Organic letters, 6, 18, 3087, 9, 02 Sep. 2004, [Peer-reviewed], [International Magazine]
English, Scientific journal

Microflora and selected metabolites of potato pulp fermented with an Indonesian starter Ragi tape
A Abe; IN Sujaya; T Sone; K Asano; Y Oda
FOOD TECHNOLOGY AND BIOTECHNOLOGY, 42, 3, 169, 173, Jul. 2004
English, Scientific journal

Comparison of Amylomyces rouxii and Rhizopus oryzae in lactic acid fermentation of potato pulp
K Saito; A Abe; IN Sujaya; T Sone; Y Oda
FOOD SCIENCE AND TECHNOLOGY RESEARCH, 10, 2, 224, 226, May 2004
English, Scientific journal

DFA III(Difructose anhydride III)投与における効果
南田公子; 金子真紀; 天野みどり; イヌガ スジャーヤ; 曽根輝雄; 和田大; 横田篤; 原博; 浅野行蔵
日本農芸化学会大会講演要旨集, 2004, 36, 05 Mar. 2004
Japanese

Identification and characterization of yeasts in brem, a traditional Balinese rice wine
IN Sujaya; NS Antara; T Sone; Y Tamura; WR Aryanta; A Yokota; K Asano; F Tomita
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 20, 2, 143, 150, Mar. 2004
English, Scientific journal

The effects of di-D-fructofuranose-1,2':2,3'-dianhydride (DFA III) administration on human intestinal microbiota.
Kimiko Minamida; I Nengah Sujaya; Akiko Tamura; Norihiro Shigematsu; Teruo Sone; Atsushi Yokota; Kozo Asano; Yoshimi Benno; Fusao Tomita
Journal of bioscience and bioengineering, 98, 4, 244, 50, 2004, [Peer-reviewed], [Domestic magazines]
English, Scientific journal

rDNA ITS sequence of Rhizopus oryzae: its application to classification and identification of lactic acid producers.
Ayumi Abe; Teruo Sone; I Nengah Sujaya; Katsuichi Saito; Yuji Oda; Kozo Asano; Fusao Tomita
Bioscience, biotechnology, and biochemistry, 67, 8, 1725, 31, Aug. 2003, [Peer-reviewed], [International Magazine]
English, Scientific journal

Speradine A, a new pentacyclic oxindole alkaloid from a marine-derived fungus Aspergillus tamarii
M Tsuda; T Mugishima; K Komatsu; T Sone; M Tanaka; Y Mikami; M Shiro; M Hirai; Y Ohizumi; J Kobayashi
TETRAHEDRON, 59, 18, 3227, 3230, Apr. 2003
English, Scientific journal

Occan, a novel transposon in the Fot1 family, is ubiquitously found in several Magnaporthe grisea isolates.
Hideki Kito; Yosuke Takahashi; Junko Sato; Satoru Fukiya; Teruo Sone; Fusao Tomita
Current genetics, 42, 6, 322, 31, Mar. 2003, [Peer-reviewed], [International Magazine]
English, Scientific journal

Modiolides A and B, two new 10-membered macrolides from a marine-derived fungus.
Masashi Tsuda; Takao Mugishima; Kazusei Komatsu; Teruo Sone; Michiko Tanaka; Yuzuru Mikami; Jun'ichi Kobayashi
Journal of natural products, 66, 3, 412, 5, Mar. 2003, [Peer-reviewed], [International Magazine]
English, Scientific journal

Lactobacillus thermotolerans sp. nov., a novel thermotolerant species isolated from chicken faeces.
Piyanuch Niamsup; I Nengah Sujaya; Michiko Tanaka; Teruo Sone; Satoshi Hanada; Yoichi Kamagata; Saisamorn Lumyong; Apinya Assavanig; Kozo Asano; Fusao Tomita; Atsushi Yokota
International journal of systematic and evolutionary microbiology, 53, Pt 1, 263, 268, Jan. 2003, [Peer-reviewed], [International Magazine]
English, Scientific journal

Casein digestion by Debaryomyces hansenii isolated from cheese.
Haruto Kumura; Kentaro Takagaki; Teruo Sone; Maki Tsukahara; Tetsuya Tanaka; Kei-ichi Shimazaki
Bioscience, biotechnology, and biochemistry, 66, 6, 1370, 3, Jun. 2002, [Peer-reviewed], [International Magazine]
English, Scientific journal

Identification of a putative vacuolar serine protease gene in the rice blast fungus, Magnaporthe grisea.
Satoru Fukiya; Takao Kuge; Tomomi Tanishima; Teruo Sone; Takashi Kamakura; Isamu Yamaguchi; Fusao Tomita
Bioscience, biotechnology, and biochemistry, 66, 3, 663, 6, Mar. 2002, [Peer-reviewed], [International Magazine]
English, Scientific journal

The production of lepidimoide by endophytic fungus from polysaccharide extracted from Abelmoshus sp. – Identification of the product and the producing organism
Biotechnology for Sustainable Utilization of Biological Resources in the Tropics, 15, 82, 87, 2002

Endophytic fungi isolated from Ulmus davidiana and their association with the host plant
Biotechnology for Sustainable Utilization of Biological Resources in the Tropics, 15, 72, 76, 2002

Establishment of a new cross of the rice blast fungus derived from Japanese differential strain Ina168 and hermaphroditic rice pathogen Guy11
S Fukiya; M Kodama; H Kito; T Sone; F Tomita
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 65, 7, 1464, 1473, Jul. 2001
English, Scientific journal

Structure and function analysis of the calcium-related gene spray in Neurospora crassa
JW Bok; T Sone; LB Silverman-Gavrila; RR Lew; FJ Bowring; DEA Catcheside; AJF Griffiths
FUNGAL GENETICS AND BIOLOGY, 32, 3, 145, 158, Apr. 2001
English, Scientific journal

Sequence analysis of porcine polymeric immunoglobulin receptor from mammary epithelial cells present in colostrum
H Kumura; T Sone; K Shimazaki; E Kobayashi
JOURNAL OF DAIRY RESEARCH, 67, 4, 631, 636, Nov. 2000
English, Scientific journal

Molecular structure of rDNA repeat unit in Magnaporthe grisea
T Sone; S Fukiya; M Kodama; F Tomita
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 64, 8, 1733, 1736, Aug. 2000
English, Scientific journal

Clonal lineages of Japanese blast fungus population - Variation of pathogenicity and karyotype
T Sone; F Tomita
ADVANCES IN RICE BLAST RESEARCH, 15, 221, 233, 2000
English, International conference proceedings

The frost gene of Neurospora crassa is a homolog of yeast cdc1 and affects hyphal branching via manganese homeostasis
T Sone; AJF Griffiths
FUNGAL GENETICS AND BIOLOGY, 28, 3, 227, 237, Dec. 1999
English, Scientific journal

Identification and characterization of a karyotypic mutation in Magnaporthe grisea
T Sone; T Abe; M Suto; F Tomita
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 61, 1, 81, 86, Jan. 1997
English, Scientific journal

DNA fingerprinting and electrophoretic karyotyping of Japanese isolates of rice blast fungus
SONE T
Ann Phytopathol Soc Jpn, 63, 3, 155, 163, 1997

Biological diversity of pyricularia oryzae.
SONE Teruo; TOMITA Fusao
KAGAKU TO SEIBUTSU, 34, 10, 676, 682, Japan Society for Bioscience, Biotechnology, and Agrochemistry, 25 Oct. 1996
Japanese

HOST SPECIES-SPECIFIC REPETITIVE DNA-SEQUENCE IN THE GENOME OF MAGNAPORTHE-GRISEA, THE RICE BLAST FUNGUS
T SONE; M SUTO; F TOMITA
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 57, 7, 1228, 1230, Jul. 1993
English

ホエイ固形培地上における食用Penicillium属由来のリパーゼ産生
佐藤恵実; 町谷泰紀; 早川徹; 曾根輝雄; 若松純一; 木村彰; 玖村朗人, 日本農芸化学会大会講演要旨集(Web), 2017, ROMBUNNO.3A05p10 (WEB ONLY), 05 Mar. 2017
Japanese

イネいもち病菌のQoI耐性変異ミトコンドリア定量系の構築
児玉葵; 阿部歩; 内橋嘉一; 鬼頭英樹; 曾根輝雄, 日本植物病理学会大会プログラム・講演要旨予稿集, 2017, 2017

アーバスキュラー菌根菌から宿主へのリン酸供給機構:PHO1リン酸排出輸送体の機能
丸山隼人; 横山楓; 菊池裕介; 中西夏輝; 阿部歩; 曾根輝雄; 斎藤勝春; 増田税; 江沢辰広, 日本土壌肥料学会講演要旨集, 62, 30, 30, 20 Sep. 2016
一般社団法人 日本土壌肥料学会, Japanese

2P-038 A histidine acid phytase homodimer from Klebsiella pneumoniae 9-3B that liberates myo-inositol from phytic acid :
Kevin Sherman; Teruo Sone; Kozo Asano, 日本生物工学会大会講演要旨集, 66, 116, 116, 2014
日本生物工学会, English

OK-007 メタン酸化菌とメタノール資化性菌の共生系におけるC1代謝遺伝子発現解析(共生・相互作用,口頭発表)
久後 貴寛; 下村 義典; 山岸 卓弥; 田中 みち子; 曾根 輝雄; 浅野 行蔵; 鎌形 洋一; 北川 航, 日本微生物生態学会講演要旨集, 29, 95, 95, 2013
日本微生物生態学会, Japanese

2P-006 Genetic relationship between antimicrobial-resistance genes and plasmids in Escherichia coli, and construction of multiple-drug resistance plasmids library
Yamamoto Shiori; Nakano Motoki; Tanaka Michiko; Kitagawa Wataru; Sone Teruo; Asano Kozo, 日本生物工学会大会講演要旨集, 65, 105, 105, 2013
日本生物工学会, Japanese

3P-065 Influence of DFAIII and Ruminococcus productus AHU1760 on intestinal environment
Takano Nanami; Fujii Sanae; Gan Ryosuke; Sone Teruo; Tanaka Michiko; Asano Kozo, 日本生物工学会大会講演要旨集, 65, 204, 204, 2013
日本生物工学会, Japanese

植物内生菌由来酵素を利用したアルカリ前処理稲わらの効率的糖化
岩井崇郎; 伊藤由美; 工藤綾子; 田中みち子; 阿部歩; 曾根輝雄; 城間力; 徳安健; 矢部希見子; 長島實; 浅野行蔵, 日本農芸化学会大会講演要旨集(Web), 2012, 2012

2Ha10 Mass production of DFA IV from sucrose by Bacillus subtilis in single culture system
Koshimoto Emi; Sone Teruo; Tanaka Michiko; Asano Kozo, 日本生物工学会大会講演要旨集, 63, 168, 168, 2011
日本生物工学会, Japanese

イネいもち病抵抗性遺伝子Piaの単離・同定
神崎洋之; 奥山雄大; 阿部陽; 吉田健太郎; 松村英生; 斉藤宏昌; 伊東明子; 曾根輝雄; 寺内良平, 育種学研究, 13, 2011

2種のNBS-LRRからなるイネいもち病抵抗性遺伝子Piaの単離・同定
神崎洋之; 奥山雄大; 阿部陽; 吉田健太郎; 松村英生; 斉藤宏昌; 伊東明子; 曾根輝雄; 寺内良平, 日本植物病理学会大会プログラム・講演要旨予稿集, 2011, 2011

イネいもち病菌の非病原性タンパク質AVR-Piaの分子間相互作用の解析
佐藤佑樹; 三木慎介; 尾瀬農之; 奥山雄大; 神崎洋之; 寺内良平; 曾根輝雄, 日本植物病理学会大会プログラム・講演要旨予稿集, 2011, 2011

Yeast Two Hybrid assayによるイネいもち病菌の非病原性遺伝子AVR-Piaのタンパク質相互作用の解析
佐藤佑樹; 三木慎介; 尾瀬農之; 奥山雄大; 神崎洋之; 寺内良平; 曾根輝雄, 日本植物病理学会報, 77, 1, 2011

Yeast Two Hybrid assayによるイネいもち病菌の非病原性遺伝子AVR-Piaのタンパク質相互作用の解析
佐藤佑樹; 三木慎介; 尾瀬農之; 奥山雄大; 神崎洋之; 寺内良平; 曾根輝雄, 日本植物病理学会大会プログラム・講演要旨予稿集, 2010, 2010

イネいもち病菌の非病原性遺伝子AVR-Pia産物の調製と分析
尾瀬農之; 笹崎容子; 三木慎介; 奥山雄大; 神崎洋之; 寺内良平; 曾根輝雄, 日本植物病理学会大会プログラム・講演要旨予稿集, 2010, 2010

イネいもち病抵抗性遺伝子Pi-aの単離とその相同遺伝子の集団遺伝学的解析
奥山雄大; 吉田健太郎; 神崎洋之; 斉藤宏昌; 阿部陽; 藤部貴宏; OLI Muluneh; 松村英生; 曾根輝雄; 寺内良平, 日本植物病理学会報, 75, 3, 2009

プロトプラスト一過的発現解析によるイネいもち病抵抗性遺伝子Pi-aの同定
神崎洋之; 奥山雄大; 吉田健太郎; 阿部陽; 松村英生; 斉藤宏昌; 曾根輝雄; 寺内良平, 育種学研究, 11, 2009

(96) Molecular Cloning of AVR-Pia, the Avirulence Gene toward the Blast Resistance Gene Pi-a in the Rice Plant(Abstracts of the Papers Presented at the Annual Meeting of the Society)
Miki S.; Ashizawa T.; Hirayae K.; Tomita F.; Sone T., Annals of the Phytopathological Society of Japan, 74, 3, 192, 192, 25 Aug. 2008
The Phytopathological Society of Japan (PSJ), Japanese

アミドトランスフェラーゼGatCAB阻害剤のin vivoスクリーニング系の構築
茶谷美穂; 中村彰良; 田中みち子; 曾根輝雄; 浅野行蔵, 日本生物工学会大会講演要旨集, 60th, 2008

2Ep16 Improvement of DFA IV production by recombinant Bacillus subtilis with expression vector contains mutational degQ gene.
TAKESUE Nobuchika; SONE Teruo; TANAKA Michiko; TOMITA Fusao; ASANO Kozo, 日本生物工学会大会講演要旨集, 20, 144, 144, 2008
日本生物工学会, Japanese

(106) Analysis of Avr-Pita Homologues in Japanese Isolates of Rice Blast Fungus(Abstracts of the Papers Presented at the Annual Meeting of the Society)
Takahashi M.; Ashizawa T.; Hirayae K.; Moriwaki J.; Sone T., Annals of the Phytopathological Society of Japan, 73, 3, 202, 203, 25 Aug. 2007
The Phytopathological Society of Japan (PSJ), Japanese

(107) Molecular cloning of Avr-Pia, the Avirulence Gene toward the Blast Resistance Gene Pi-a in the Rice Plant(Abstracts of the Papers Presented at the Annual Meeting of the Society)
Miki S.; Ashizawa T.; Hirayae K.; Tomita F.; Sone T., Annals of the Phytopathological Society of Japan, 73, 3, 203, 203, 25 Aug. 2007
The Phytopathological Society of Japan (PSJ), Japanese

2D17-4 Production of DFA IV by recombinant Bacillus subtilis carrying levan fructotransferase from Arthrobacter nicotinovorans GS-9
TAKESUE Nobuchika; SONE Teruo; TANAKA Michiko; TOMITA Fusao; ASANO Kozo, 日本生物工学会大会講演要旨集, 19, 107, 107, 2007
日本生物工学会, Japanese

イネいもち病菌の非病原性遺伝子Avr-piaのクローニング
曾根輝雄; 三木慎介; 松井幸太郎; 芹澤武人; 鬼頭英樹; 平八重一之; 中島敏彦; 冨田房男, 植物微生物研究会研究交流会講演要旨集, 16th, 2007

(61) Analysis of Avr-Pita Sequences in Virulent Mutants from Pyricularia grisea isolate OS99-G-7a(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)
Takahashi M.; Hirayae K.; Ashizawa T.; Moriwaki J.; Sone T.; Sonoda R.; Noguchi M. T., Annals of the Phytopathological Society of Japan, 72, 4, 218, 218, 25 Nov. 2006
The Phytopathological Society of Japan (PSJ), Japanese

(60) Molecular Gloning of Avr-Pia, the Avirulence Gene in Magnaporthe grisea toward the Rice Blast Resistance Gene Pi-a(Abstract of the Paper Presented at the 2006 Annual Meeting in Sapporo)
Miki S.; Matsui K.; Ashizawa T.; Kito H.; Hirayae K.; Nakajima T.; Tomita F.; Sone T., Annals of the Phytopathological Society of Japan, 72, 4, 218, 218, 25 Nov. 2006
The Phytopathological Society of Japan (PSJ), Japanese

イネいもち病菌の非病原性遺伝子Avr-Piaのクローニング
三木慎介; 松井幸太郎; 鬼頭英樹; 吹谷智; 佐藤順子; 平八重一之; 中島敏彦; 冨田房男; 曾根輝雄, 日本農芸化学会大会講演要旨集, 2006, 2006

3G13-5 Isolation and characterization of filamentous fungi from fermented food starters in Thailand and Korea
ABE Ayumi; ODA Yuji; SONE Teruo; ASANO Kozo, 日本生物工学会大会講演要旨集, 17, 210, 210, 2005
日本生物工学会, Japanese

(2) Analyses of Mini-chromosome in the Rice Blast Fungus : (1) Identification of Repetitive Sequence(Abstracts of the Papers Presented at the Annual Meeting of the Society, Fukuoka, March 28-30, 2004)
Sone T.; Oguchi A.; Kikuchi H.; Senoh A.; Nakagawa S.; Tomita F., Annals of the Phytopathological Society of Japan, 70, 3, 190, 190, 25 Aug. 2004
The Phytopathological Society of Japan (PSJ), Japanese

アジア発酵食品由来のRhizopus oryzaeとAmylomyces rouxiiの有機酸生成能と乳酸脱水素酵素(ldh)遺伝子による特徴づけ
鬼頭英樹; 斎藤勝一; 阿部歩; 曾根輝雄; 浅野行蔵; 小田有二, 日本農芸化学会大会講演要旨集, 2004, 2004

Analyses of DNA-type Transposon Occan in the Rice Blast Fungus(Abstracts of the Papers Presented at the 2003 Annual Meeting in Tokyo)
Takahashi Y.; Kito H.; Sone T.; Tomita F., Annals of the Phytopathological Society of Japan, 69, 3, 237, 237, 25 Aug. 2003
The Phytopathological Society of Japan (PSJ), Japanese

AFLP analysis of Rhizopus oryzae and Amylomyces rouxii for the Taxonomic study
Sone Teruo; Abe Ayumi; Nengah Sujaya; Saito Katsuichi; Oda Yuji; Asano Kozo, 日本生物工学会大会講演要旨集, 15, 132, 132, 2003
日本生物工学会, Japanese

Microflora analysis of potato-pulp fermented with Ragi tape as a starter
Abe Ayumi; Sone Teruo; Sujaya I Nengah; Saito Katsuichi; Oda Yuji; Asano Kozo, 日本生物工学会大会講演要旨集, 15, 117, 117, 2003
日本生物工学会, Japanese

イネいもち病菌の新規トランスポゾンOccanの特徴
曾根輝雄; 鬼頭英樹; 高橋洋介; 佐藤順子; 冨田房男, 日本農芸化学会大会講演要旨集, 2003, 2003

リモニウムペレジー(Limonium perezii)の発根を促進する微生物
イ・ヌガ スジャーヤ; 田中みち子; 曽根輝雄; 和田大; 浅野行蔵; 横田篤, 日本農芸化学会北海道支部・北海道農芸化学協会シンポジウム及び合同学術講演会講演要旨, 2003, 2003

(74)Mutations in Avr-pita Identified in Japanese Isolates of Magnaporthe grisea
Sone T.; Tomita F., Annals of the Phytopathological Society of Japan, 68, 2, 176, 176, 25 Aug. 2002
The Phytopathological Society of Japan (PSJ), Japanese

(75)Molecular Cloning of the Avirulence Gene Avr-pia from Magnaporthe grisea by Utilizing of the Host Specificity Mutant
Kito H.; Takahashi Y.; Fukiya S.; Sato J.; Sone T.; Iwano M.; Nakajima T.; Tomita F., Annals of the Phytopathological Society of Japan, 68, 2, 176, 176, 25 Aug. 2002
The Phytopathological Society of Japan (PSJ), Japanese

(76)A New DNA-Type Transposon Occan of Magnaporthe grisea
Takahashi Y.; Kito H.; Sato J.; Fukiya S.; Sone T.; Tomita F., Annals of the Phytopathological Society of Japan, 68, 2, 176, 176, 25 Aug. 2002
The Phytopathological Society of Japan (PSJ), Japanese

711 The improvement in the growth of Aspergillus niger M3 resulted to high production of phytase
Qi Wang; Sone Teruo; Asano Kozo; Tomita Fusao, 日本生物工学会大会講演要旨集, 14, 121, 121, 2002
日本生物工学会, Japanese

411 Taxonomic diversity of salt-tolerant yeasts from miso mash
Tanaka Takayuki; Sujaya I Nengah; Tamura Yoshifumi; Yamaki Tazusa; Sone Teruo; Yokota Atsushi; Asano Kouzou; Tomita Fusao, 日本生物工学会大会講演要旨集, 14, 53, 53, 2002
日本生物工学会, Japanese

402 Analysis of the fecal microbiota of human-subjects consuming DFA3
Minamida Kimiko; Nengah Sujaya; Sone Teruo; Asano Kozo; Yokota Atsushi; Tomita Fusao, 日本生物工学会大会講演要旨集, 14, 51, 51, 2002
日本生物工学会, Japanese

406 Rapid selection of lactic acid-forming R. oryzae by selective PCR
Abe Ayumi; Sone Teruo; Sujaya I Nengrah; Saito Katsuichi; Oda Yuji; Asano Kozo; Tomita Fusao, 日本生物工学会大会講演要旨集, 14, 52, 52, 2002
日本生物工学会, Japanese

変異株を利用したいもち病菌非病原性遺伝子の取得
鬼頭英樹; 高橋洋介; 吹谷智; 佐藤順子; 岩野正敬; 曽根輝雄; 冨田房男, 日本農芸化学会大会講演要旨集, 2002, 2002

Identification and Cloning of a Putative Serine Protease Gene from the Rice Blast Fungus, Magnaporthe grisea
Fukiya S.; Kuge T.; Tanishima T.; Sone T.; Kamakura T.; Yamaguchi I.; Tomita F., Annals of the Phytopathological Society of Japan, 67, 2, 131, 132, 25 Aug. 2001
The Phytopathological Society of Japan (PSJ), Japanese

変異株を利用したいもち病菌非病原性遺伝子連鎖マーカーの取得
鬼頭英樹; 吹谷智; 佐藤順子; 谷島智美; 岩野正敬; 曽根輝雄; 冨田房男, 日本農芸化学会誌, 75, 2001

306 Characterization of Yeast Strains in Alcoholic Fermentation of Brem :
Sujaya I. Nengah; Sone T.; Yokota A.; Asano K.; Tomita F., 日本生物工学会大会講演要旨集, 12, 59, 59, 2000
日本生物工学会, English

(175) Analysis of the Deleted Region of Host Specificity Mutants
Sato J.; Fukiya S.; Sone T.; Iwano M.; Tomita F., Annals of the Phytopathological Society of Japan, 64, 4, 370, 370, 25 Aug. 1998
The Phytopathological Society of Japan (PSJ), Japanese

(173) DNA Finger printing of Japanese Isolates of Rice Blast Fungus
Sone T.; Fukiya S.; Iwano M.; Koizumi S.; Fujita Y.; Tomita F., Annals of the Phytopathological Society of Japan, 64, 4, 370, 370, 25 Aug. 1998
The Phytopathological Society of Japan (PSJ), Japanese

(215) Establishment of a New Cross System for Japanese Rice Pathogens of Rice Blast Fungus(Abstracts of the Papers Presented at the Annual Meeting of the Society)
FUKIYA S.; SONE T.; ABE T.; KODAMA M.; SUTO M.; TOMITA F., Annals of the Phytopathological Society of Japan, 63, 3, 244, 245, 25 Jun. 1997
The Phytopathological Society of Japan (PSJ), Japanese

A Molecular Genetic Approach to Pathogenic-race Variation of Rice Blast Fungus
SONE T.; ABE T.; FUKIYA S.; KODAMA M.; IWANO M.; KOIZUMI S.; FUJITA Y.; TOMITA F., Annals of the Phytopathological Society of Japan, 62, 3, 294, 294, 25 Jun. 1996
The Phytopathological Society of Japan (PSJ), Japanese

Genetic analysis of rice blast fungus-Establishment and application to Japanese isolates
SONE Teruo; TOMITA Fusao, Bioscience & industry, 54, 5, 11, 16, 01 May 1996
バイオインダストリ-協会, Japanese

いもち病菌の高稔性交配系を用いた遺伝子解析 : 微生物
曽根 輝雄; 吹谷 智; 阿部 卓巳; 児玉 恵; 須藤 学; 冨田 房男, 日本農藝化學會誌, 70, 117, 117, 05 Mar. 1996
社団法人日本農芸化学会, Japanese

イネいもち病菌による交配実験系の確立 : 微生物
曽根 輝雄; 吹谷 智; 阿部 卓巳; 吉田 奈美; 児玉 恵; 須藤 学; 富田 房男, 日本農藝化學會誌, 69, 273, 273, 05 Jul. 1995
社団法人日本農芸化学会, Japanese

ワンダーフォーゲル実習Ⅵ, 2024年, 博士後期課程, 国際食資源学院

人口・食料・環境学総論, 2024年, 修士課程, 農学院

食資源特別演習, 2024年, 修士課程, 国際食資源学院

大学院共通授業科目（一般科目）：複合領域, 2024年, 修士課程, 大学院共通科目

食資源生産論, 2024年, 修士課程, 国際食資源学院

持続的生物生産技術特論, 2024年, 修士課程, 国際食資源学院

大学院共通授業科目（一般科目）：自然科学・応用科学, 2024年, 修士課程, 大学院共通科目

食資源特別講義, 2024年, 修士課程, 国際食資源学院

大学院共通授業科目（教育プログラム）：教養深化プログラム, 2024年, 修士課程, 大学院共通科目

環境と人間, 2024年, 学士課程, 全学教育

HSI extension/professional program, 2024年, 学士課程, HSI

生物学実験Ⅰ, 2024年, 学士課程, 農学部

応用菌学, 2024年, 学士課程, 農学部

一般教育演習(ﾌﾚｯｼｭﾏﾝｾﾐﾅｰ), 2024年, 学士課程, 全学教育

Elucidation of QoI resistant mutation mechanism in rice blast fungus
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
01 Apr. 2018 - 31 Mar. 2021
Sone Teruo
This study aimed to clarify the mechanism of emergence, inheritance and distribution of QoI fungicide resistance in rice blast fungus. We could not conclude about the mechanism of mutation emergence, due to the difficulty of mutation induction in vitro. On the other hand, visualization of mitochondria with GFP was succeed, and it enabled to observe that only a few mitochondria were distributed to the conidia as tubule form, and they are divided into dots simultaneously with the septum formation between conidia and conidiophore. It suggested us that conidiation has a role for the rapid distribution of the mutant.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 18K05640

New insight into plant acid tolerance: physiological and molecular mechanisms of acid tolerance through mycorrhizal formation
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2017 - 31 Mar. 2020
Ezawa Tatsuhiro
Soil acidity is a major constraint of plant productivity. The objective of this study was understanding of the mechanism underlying the improved acid tolerance by mycorrhizal formation. The arbuscular mycorrhizal fungus Rhizophagus clarus strain RF1 isolated from acidic soil improved plant survival and growth in acidic soil, which accompanied the upregulation of genes involved in radical scavenging and magnesium transport. In addition, the abundance of a symbiotic mycovirus RcMV3 was also increased in response to soil acidity. These observations suggest that the improvement of magnesium homeostasis via upregulation of the transporter gene and general stress tolerance by viral symbiosis are involved in the acid tolerance of the strain.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 17H03779

Comparison of mutation frequency of the avirulence gene AVR-Pia introduced to different genomic region of Pyricularia oryzae.
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
01 Apr. 2016 - 31 Mar. 2019
Chuma Izumi; Arazoe Takayuki; Sone Teruo; Le Dinh Don; Nguyen Thi Thin Nga; Cumagun Christian; Sreewongchai Tanee; Spring Otmar
We compared frequency of mutation of the avirurence gene AVR-Pia which was introduced to repeat rich (RR) and repeat poor (RP) regions of rice blast fungus, Pyricularia oryzae. Resistant rice cultivar Aichi-asahi (Pia) were inoculated with transformants ta#126 which has AVR-Pia in RR region and zt#1 which has AVR-Pia in RP region. When compared mutants isolated from susceptible lesions of inoculated rice leaves, mutation frequency of ta#126 was higher than that of zt#1. Mutation mechanism of all mutants were deletion of introduced AVR-Pia. The deleted region were surrounded by transposable elements and its size depends on the distance between transposable elements.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), 16K07615

Elucidation of molecular mechanism of rice blast resistance
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
01 Apr. 2011 - 31 Mar. 2014
SONE Teruo; TERAUCHI Ryohei; OSE Toyoyuki
Rice blast is the most important disease or rice. Blast is usually controlled by agrochemicals and resistant cultivars, but knowledge about molecular mechanisms of rice resistance toward blast is limited. This study aimed to elucidate the molecular mechanism of rice blast resistance by analyzing interaction of rice blast resistance gene Pia and blast virulence gene AVR-Pia. Results of this study indicated that AVR-Pia gene is started to be expressed before the penetration into rice cells, and after the secretion, AVR-Pia protein forms homodimer, and detected by rice Pia gene product. This study will contribute to further understanding of rice-rice blast interaction, which is the first step of resistance induction.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 23380024

Development of novel rice blast control system, which suppresses mutations in the pathogen
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
2011 - 2013
SONE Teruo
Rice blast is the most important disease of rice. One of the big problem in rice blast control is the mutation of pathogen that enables pathogens to overcome rice blast resistance. Mutations in rice blast fungus is mostly occur through DNA recombinations. This study aimed to develop a novel rice blast control strategy, which suppresses mutations in the pathogens, by studying the DNA recombination proteins in the pathogen. Results of this study indicated that DNA recombination is very important for the pathogen to cause the disease. DNA recombination proteins works as a complex will be a target for controlling the disease and suppressing the mutations. In this study, we also developed a screening system for inhibitors for protein-protein interaction.
Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, 23658038

Analysis of AVR-Pia gene of trice blast fungus and its application for the pathogenicity differentiation
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
2008 - 2010
SONE Teruo
Successful molecular cloning and identification of AVR-Pia avirulence gene from Magnaporthe oryzae was demonstrated in this study. AVR-Pia gene was located at the same position as the locus which was genetically analyzed using Chinese isolates. AVR-Pia was expressed only during infection, and the product was found to be secreted into compatible host cells, through BICs of the infection hyphae. Mechanism of gene deletion in the mutant Ina168m95-1 was elucidated to be the homologous recombination between two adjacent copies of the DNA transposon Occan. PCR amplification of the gene is effective way to determine the pathogenicity toward rice cultivar with Pia R gene.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 20580043

フィターゼとその遺伝子に関する研究
その他の研究制度
2008
Competitive research funding

Studies on phytases and their genes
The Other Research Programs
2008
Competitive research funding

Development of cadmium releasing technology from marine waste using the metallothionein proteolytic enzyme.
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
2004 - 2005
ASANO Kozo; SONE Teruo
In organisms, cadmium is bound to specific protein, metallothioneins (MTs), which are cystein-rich, low-molecular-weight, metal-binding proteins. If MTs can be specifically hydrolyzed by a protease, we can easily remove cadmium from scallop hepatopancreas ; a marine waste. Microbial treatments have the potential to clean up marine waste by ecological processes. In addition, because a protease which can specifically hydrolyze MTs is still unknown, the enzyme is expected to have unique characteristics.
Our study found Arthrobacter nicotnovorans 23-0-11 from soil as a microbe producing a protease which can release cadmium from scallop hepatopancreas into liquid medium. The protease was purified with two types of ion-exchange chromatographies. The molecular mass of the purified protease was estimated to be 27 kDa.
The optimum pH and temperature of the enzyme was pH7.0 and 50℃, respectively. The activity was stable from pH3.0 to 9.0, and at temperature below 60℃. Sensitivity to protease inhibitors indicated that this protease belongs to serine protease family.
Partial amino acid sequences were obtained from the N-terminal and the internal parts of the protease. Degenerate oligo-nucleotide primers were designed from those sequences and PCR was carried out. The nucleotide sequence of the PCR product was used as an aid to obtain whole nucleotide sequence of the protease.
The amino acid sequence of the protease had the conserved motif of the serine proteases. Although amino acid sequences of some known proteases were retrieved from the database, their homologies were not so high.
Mass production of the protease was performed using E.coli as a host. Although the protease gene was cloned and introduced into E.coli, the protease was expressed as the insoluble and inactive form. Therefore, further considerations of the vector, host and conditions for expression are needed.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 16580049

イネいもち病菌はいつどこで変異するのか
科学研究費助成事業 若手研究(B)
2004 - 2005
曾根 輝雄
1.新たな遺伝子のクローニング
新たに非相同末端再結合に関わる遺伝子であるKu70,Ku80のいもち病菌ホモログ,Khm70,Khm80をPCRにより,日本産イネいもち病菌Ina168株より取得した.これらはこれまでにクローニングしていた遺伝子と同様,アカパンカビ(Neurospora crassa)のホモログと最も高い相同性を示した.
2.N.crassa変異株の相補による機能の確認
これまでにクローニングしたRhm52,Rhm54,Mhm11,Khm70,Khm80をハイグロマイシン耐性プラスミドベクターpCSN43-DESTに連結し,それぞれN.crassaの変異株mus-11,mus-25,mus-23,mus-51,mus-52に導入し,変異の相補により遺伝子の機能を確認した.その結果,Rhm52,Rhm54,Khm80は相補が確認され,機能を持った遺伝子であることが示唆された.Mhm11,Rhm70は相補が見られなかったが,これらは複合体を形成し機能する遺伝子であり,その複合体の形成に問題があることが考えられた.
3.欠損変異株の作成
昨年度開発したベクターをpDESTRと命名し,さらに同様の,ビアラフォス耐性遺伝子を選択マーカーとして持ったベクター,pBARSTを作成した.これらのベクターを使用し,Inverse PCR法によりRhm54,Rhm52,Khm70,Khm80破壊コンストラクトを作成した.10株の形質転換体から,欠損変異株を得ることができ,ベクターの有効性が証明された.
日本学術振興会, 若手研究(B), 北海道大学, 16780029

赤カビ毒（DON, NIV）のアセチル化酵素の特性の解明とその応用
2005
Competitive research funding

Studies on trichothecene acetyl transferases
2005
Competitive research funding

Study on the induction system of host specificity mutations in Magnaporthe grisea,: avirulence genes and recombinational repair genes
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
1999 - 2001
TOMITA Fusao; YASUDA Nobuko; NAKAJIMA Toshihiko; ISHII Chizu; SONE Teruo; TSUJIMOTO Masako
(1) Analysis of avirulence genes in M. grisea
Three avirulence genes, Avr-pii, Avr-pia, Avr-Hattan3 was investigated using a cross between two Yunnang isolates. These avirulence genes were mapped onto the genetic map of the fungus. Along the Avr-Hattan 3, a BAC contig of 600kb in length was constructed. On the other hand, Avr-pia was identified also from a cross between Japanese isolate and rare hermaphloditic isolate Guy11. By utilization of the host specificity mutant, a DNA fragment named PM-01, which was tightly linked to the Avr-pia locus was isolated. One of the cosmid clone containing the PM-01 fragment was found to be able to complement the host specificity mutation, indicating that the clone containing the Avr-pia gene.
(2) Analysis of recombnational repair genes in M. grisea
Homologous genes for RAD52, RAD54, and MRE11 was cloned from M. grisea genome, and named Rhm52, Rhm54 and Mhm11, respectively. Among these homologs, Rhm54 was found to complement the phenotype of mus-23 mutant of N. crassa, and to be inducible by MMS treatment in M. grisea cells.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Hokkaido University, 11306007

Rhizopus属菌の分子系統解析とその応用
2001
Competitive research funding

Molcular taxonomy of the genus Rhizopus and its application
2001
Competitive research funding

いもち病菌の染色体再編成による病原性レース変異機構の解析
科学研究費助成事業 特別研究員奨励費
1998 - 2000
曽根 輝雄
日本学術振興会, 特別研究員奨励費, 北海道大学, 98J02369

いもち病菌の分子遺伝学的解析による病原性レース変異機構の解明
1991
Competitive research funding

Molecular genetic analysis of Magnaporthe grisea host specificity and mutations
1991
Competitive research funding

アルカリジェネス属の微生物の生菌を主成分とする植物発根促進剤
Patent right
特許出願２００４−１１７３０３