Researcher basic information

• 博士（歯学）, 北海道大学

• https://researchmap.jp/read0166705?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200901016270091107

• 200901016270091107

• 歯内療法
• 歯周病予防
• 歯周外科
• 歯内療法学
• Endodontics
• Prevention of Periodontal Disease
• Periodontal surgery

• Life Science, Conservative dentistry

• Bachelor's degree program, School of Dental Medicine

Research activity information

• 2008, 日本歯科理工学会会誌賞
  Japan
• 2006, 日本歯周病学会会誌賞
  Japan
• 1996, 日本歯周病学会奨励賞
  Japan

• 垂直歯根破折歯の長期保存を目指した接着治療の臨床アプローチ
  菅谷 勉
  歯科医療, 夏, 18, 24, Jul. 2026, [Invited]
  Japanese
• Impact of electrode distance, axial position, and energization time on cauterization of root canal isthmuses using high-frequency current
  Ahmed Saud Alkuwaykibi; Tsutomu Sugaya; Mohammed Katib Alruwaili; Naoki Takahashi
  Clinical Oral Investigations, 30, 6, Springer Science and Business Media LLC, 03 Jun. 2026, [Peer-reviewed]
  English, Scientific journal
• メタクリル酸エステル系接着性シーラーの封鎖性と生体親和性に及ぼす水の影響
  鈴木 魁; 鷲巣太郎; 菅谷 勉
  日保存誌, 69, 1, 21, 32, Feb. 2026, [Peer-reviewed], [Corresponding author]
  Japanese, Scientific journal
• 清掃状態が不良な歯周病に対するCPC製剤の洗口とスケーリングの併用による臨床効果
  竹生 寛恵; 工藤 愛; 下地 伸司; 小田島 朝臣; 加藤 昭人; 鷲巣 太郎; 町田 紗樹; 高橋 直紀; 田中 佐織; 菅谷 勉
  日本歯周病学会会誌, 67, 秋季特別, 149, 149, (NPO)日本歯周病学会, Oct. 2025
  Japanese
• A cross-sectional interventional study on the effects of periodontal treatment on periodontal inflamed surface area and masticatory efficiency values according to the 2018 periodontal status classification
  Shinji Matsuda; Hiromichi Yumoto; Yasutaka Komatsu; Nanae Dewake; Takanori Iwata; Takatoshi Nagano; Toshiya Morozumi; Ryoma Goto; Satsuki Kato; Motozo Yamashita; Joichiro Hayashi; Satoshi Sekino; Akiko Yamashita; Keiko Yamashita; Atsutoshi Yoshimura; Tsutomu Sugaya; Shogo Takashiba; Yoichiro Taguchi; Eiji Nemoto; Tomoaki Shintani; Tsuyoshi Miyagawa; Hiromi Nishi; Noriyoshi Mizuno; Yukihiro Numabe; Hiroyuki Kawaguchi
  BMC Oral Health, 25, 1, Springer Science and Business Media LLC, 04 Jul. 2025
  Scientific journal
• Pharmacokinetics and the effectiveness of pyrogen-free bioabsorbable wet adhesives.
  Risa Oshima; Kumiko Yoshihara; Ko Nakanishi; Tsukasa Akasaka; Shinji Shimoji; Teppei Nakamura; Takumi Okihara; Mariko Nakamura; Akihiro Matsukawa; Ikkei Tamada; Bart Van Meerbeek; Tsutomu Sugaya; Yasuhiro Yoshida
  Scientific reports, 15, 1, 20056, 20056, 12 Jun. 2025, [International Magazine]
  English, Scientific journal, Bioabsorbable materials are essential for advanced therapies, including surgical sealing, cell therapy, and drug delivery. Natural bioabsorbable materials, including collagen and hyaluronic acid, have better biocompatibility than synthetic bioabsorbable polymers; however, they are mainly derived from animals, presenting infection risks. Non-animal origin polymers have a lower molecular weight than those of animal origins. Their viscosity increases with increase in molecular weight, making endotoxin removal difficult. Here, using the phosphoryl chloride disposal method, we present a strategy for synthesizing pyrogen-free bioabsorbable adhesives with controlled molecular weight. Phosphopullulan, a polysaccharide derivative, had less than detectable endotoxin levels and controllable average molecular weight of approximately 300,000 to over 1,400,000. Furthermore, it is important to ensure the safety as well as efficacy of bio-implantable materials. We have evaluated the biosafety of polysaccharide derivatives we are developing, and have examined their cell phagocytosis and pharmacokinetics in vitro and in vivo, and have confirmed that they are safe. We have also evaluated their adhesion to wet tissue adhesions and confirmed that they leak less than existing materials.
• Effects of Low-intensity Pulsed Ultrasound on Bone Formation with Gelatin Hydrogel Containing Bone Morphogenetic Protein-2
  Yamaji Kozo; Yokoyama Akihito; Sugaya Tsutomu; Matsuzaki Kumiko; Takahashi Kei; Shinno Yasuo; Ohara Naoko; Suzuki Shigeki
  J Oral Tissue Engin, 22(2), Dec. 2024, [Peer-reviewed]
  English, Scientific journal
• 垂直歯根破折の接着治療と予後に影響する要因
  菅谷 勉
  日本顎咬合学会誌: 咬み合わせの科学, 44, 1, 5, 13, (NPO)日本顎咬合学会, Aug. 2024, [Peer-reviewed]
  Japanese
• 接着を活用した歯の保存戦略 垂直歯根破折の接着治療と長期予後
  菅谷 勉
  日本歯科保存学雑誌, 67, 2, 89, 93, (NPO)日本歯科保存学会, Apr. 2024, [Peer-reviewed]
  Japanese
• Effect of Periodontal Treatment on Reducing Chronic Inflammation in Systemically Healthy Patients With Periodontal Disease.
  Shinji Matsuda; Tomoaki Shintani; Tsuyoshi Miyagawa; Hiromichi Yumoto; Yasutaka Komatsu; Nanae Dewake; Takanori Iwata; Takatoshi Nagano; Toshiya Morozumi; Ryoma Goto; Satsuki Kato; Masahiro Kitamura; Kitetsu Shin; Satoshi Sekino; Akiko Yamashita; Keiko Yamashita; Atsutoshi Yoshimura; Tsutomu Sugaya; Shogo Takashiba; Yoichiro Taguchi; Eiji Nemoto; Hiromi Nishi; Noriyoshi Mizuno; Yukihiro Numabe; Hiroyuki Kawaguchi
  The American journal of medicine, 137, 3, 273, 279, Mar. 2024, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: We determined the effects and an accurate marker of periodontal treatment on serum interleukin (IL)-6 and high-sensitivity C-reactive protein (HsCRP) levels in systemically healthy individuals with periodontal disease. METHODS: This multicenter study included systemically healthy individuals with periodontal disease who received initial periodontal treatment and had no periodontal treatment history. Periodontal parameters, including periodontal inflamed surface area, masticatory efficiency, and periodontal disease classification; serum IL-6 and HsCRP levels; and serum immunoglobulin (Ig)G titers against periodontal pathogens were evaluated at baseline and after treatment. Subjects were classified as low or high responders (group) based on periodontal inflamed surface area changes. RESULTS: There were 153 participants. Only periodontal inflamed surface area changes were markedly different between low and high responders. Periodontal treatment (time point) decreased both serum IL-6 and HsCRP levels. The interaction between group and time point was remarkable only for serum IL-6 levels. Changes in serum immunoglobulin (Ig)G titers against periodontal pathogens were not associated with IL-6 changes in high responders. We analyzed the indirect effect of serum anti-Porphyromonas gingivalis type 2 IgG titer changes using mediation analysis and found no significance. However, the direct effect of group (low or high responder) on IL-6 changes was considerable. CONCLUSIONS: Periodontal treatment effectively decreased serum IL-6 levels, independent of periodontal pathogen infection, in systemically healthy individuals with periodontal disease.
• Can a low dosage of recombinant human bone morphogenetic protein-2 loaded on collagen sponge induce ectopic bone?
  Mohammed Katib Alruwaili; Tsutomu SUGAYA; Yasuhito MORIMOTO; Ko NAKANISHI; Tsukasa AKASAKA; Yasuhiro YOSHIDA
  Dental Materials Journal, 42, 3, 433, 440, Japanese Society for Dental Materials and Devices, 25 May 2023, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Recombinant human bone morphogenetic protein-2 (rhBMP-2) is one of the growth factors that may induce the formation of new bone. The aim was to determine the efficacy of low doses of rhBMP-2 for bone regeneration using a collagen sponge as a carrier. Three doses of rhBMP-2 (1.167, 0.117, and 0.039 mg/mL) were combined with an absorbable collagen sponge (ACS) as a delivery vehicle. The rhBMP-2/ACS implants were placed in the subcutaneous tissues of rat backs. X-ray microcomputed tomography (micro-CT) and histological analysis were used to evaluate bone formation. The samples treated with 1.167 mg/mL of rhBMP-2 showed greater bone formation than the samples treated with 0.117 mg/mL of rhBMP-2 four weeks after surgery. However, there was no evidence of bone formation in the samples that were treated with 0.039 mg/mL of rhBMP-2. It was found that rhBMP-2 was osteogenic even at one-tenth of its manufacturer's recommended concentration (1.167 mg/mL), indicating its potential for clinical use at lower concentrations.
• ラット骨窩洞内に埋植した水酸化カルシウム系根管充填剤に対する組織反応
  田中 佐織; 宮治 裕史; 加藤 昭人; 金本 佑生実; 菅谷 勉
  特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 158回, 84, 84, (NPO)日本歯科保存学会, May 2023
  Japanese
• ラット骨窩洞内に埋植した水酸化カルシウム系根管充填剤に対する組織反応
  田中 佐織; 宮治 裕史; 加藤 昭人; 金本 佑生実; 菅谷 勉
  特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 158回, 84, 84, (NPO)日本歯科保存学会, May 2023
  Japanese
• 酸化亜鉛ユージノール系ペーストタイプ根管充填シーラーの材料特性ならびに生体親和性評価
  加藤 昭人; 宮治 裕史; 金本 佑生実; 蔀 佳奈子; 岡本 一絵; 吉野 友都; 浜本 朝子; 西田 絵利香; 菅谷 勉; 田中 佐織
  日本歯科保存学雑誌, 66, 2, 114, 123, (NPO)日本歯科保存学会, Apr. 2023
  Japanese
• ヒトI型コラーゲン様リコンビナントペプチド/β-三リン酸カルシウム骨補填材による歯周組織再生効果
  吉野 友都; 宮治 裕史; 西田 絵利香; 金本 佑生実; 浜本 朝子; 加藤 昭人; 菅谷 勉; 赤坂 司
  日本歯周病学会会誌, 65, 春季特別, 133, 133, (NPO)日本歯周病学会, Apr. 2023
  Japanese
• 全身性疾患への影響を考慮した新たな歯周病重症度検査項目の策定 学会主導型多施設臨床研究(第二報)
  松田 真司; 菅谷 勉; 加藤 幸紀; 根本 英二; 竹内 康雄; 山下 慶子; 沼部 幸博; 西田 哲也; 小方 頼昌; 申 基哲; 長野 孝俊; 両角 俊哉; 小松 康高; 出分 菜々衣; 後藤 亮真; 北村 正博; 田口 洋一郎; 高柴 正悟; 湯本 浩通; 山下 明子; 吉永 泰周; 吉村 篤利; 河口 浩之
  日本歯周病学会会誌, 65, 春季特別, 129, 129, (NPO)日本歯周病学会, Apr. 2023
  Japanese
• ヒトI型コラーゲン様リコンビナントペプチド/β-三リン酸カルシウム骨補填材による歯周組織再生効果
  吉野 友都; 宮治 裕史; 西田 絵利香; 金本 佑生実; 浜本 朝子; 加藤 昭人; 菅谷 勉; 赤坂 司
  日本歯周病学会会誌, 65, 春季特別, 133, 133, (NPO)日本歯周病学会, Apr. 2023
  Japanese
• 酸化亜鉛ユージノール系ペーストタイプ根管充填シーラーの材料特性ならびに生体親和性評価
  加藤 昭人; 宮治 裕史; 金本 佑生実; 蔀 佳奈子; 岡本 一絵; 吉野 友都; 浜本 朝子; 西田 絵利香; 菅谷 勉; 田中 佐織
  日本歯科保存学雑誌, 66, 2, 114, 123, (NPO)日本歯科保存学会, Apr. 2023
  Japanese
• Cauterization of Narrow Root Canals Untouched by Instruments by High-Frequency Current
  Hiromichi Kumagai; Tsutomu Sugaya; Toshihiko Tominaga
  Materials, 16, 7, 2542, 2542, MDPI AG, 23 Mar. 2023, [Peer-reviewed], [Corresponding author]
  English, Scientific journal, The mechanical removal of bacteria is fundamental to the treatment of infected root canals, but complete sterilization of biofilms tends not to extend to uninstrumented areas. However, during electrical conduction to a root canal filled with a conductor, the higher impedance where the root canal is narrower generates Joule heat that may result in a large temperature increase and sterilization. The effect of a high-frequency electric current on the wall of a simulated narrow root canal was investigated by scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS). Simulated root canals, 0.1 mm in diameter, were prepared in dentine blocks. The root canal wall was treated with Plank-Rychlo solution for 5 min to create a decalcified layer. The simulated root canal was filled with either saline or NaClO, and 150 or 225 V at 520 kHz was applied for 0 s, 1 s, or 5 s. As the conduction time increased, and when the saline was replaced with NaClO, the proportion with a flat decalcified surface decreased, dentinal tubules and a lava-like morphology were significantly more evident on SEM (p < 0.01), and EDS showed significant decreases in carbon and oxygen and increases in calcium (p < 0.01). It was concluded that filling uninstrumented root canals with NaClO and using electrical conduction for 5 s could incinerate and eliminate the organic material of the root canal wall. The application of high-frequency electric current may lead to the cure of many cases of persistent apical periodontitis.
• Water-resistant antibacterial properties of a graphene oxide/cetylpyridinium chloride complex formed on medical gauze fibers.
  Yukimi Kanemoto; Hirofumi Miyaji; Erika Nishida; Asako Hamamoto; Tsutomu Sugaya; Syun Gohda; Hironobu Ono
  Journal of oral biosciences, 19 Feb. 2023, [International Magazine]
  English, Scientific journal, OBJECTIVES: Graphene oxide (GO) is a nanocarbon material with a high aspect ratio (width:thickness) and abundant anionic functional groups on its surface. In this study, we attached GO to the surface of medical gauze fibers, constructed a complex with a cationic surface active agent (CSAA), and demonstrated that the treated gauze exhibits antibacterial activity even after rinsing with water. METHODS: Medical gauze was immersed in GO dispersion (0.001%, 0.01%, and 0.1%), rinsed with water, dried, and subjected to the Raman spectroscopy analysis. Subsequently, the gauze treated with 0.001% GO dispersion was immersed in 0.1% cetylpyridinium chloride (CPC) solution, immediately rinsed with water, and dried. Untreated, GO-only, and CPC-only gauzes were prepared for comparison. Each gauze was placed in a culture well, seeded with Escherichia coli or Actinomyces naeslundii, and turbidity was measured after 24 h of incubation. RESULTS: The Raman spectroscopy analysis of the gauze after immersion and rinsing showed a G-band peak, indicating that GO remained on the surface of the gauze. The turbidity measurements indicated that GO/CPC-treated gauze (GO-treated and rinsed, followed by CPC-treatment and rinsing) significantly decreased turbidity compared to the other gauzes (P<0.05), suggesting that the GO/CPC complex remained on the gauze fibers even after water rinsing and showed antibacterial activity. CONCLUSIONS: The GO/CPC complex imparts water-resistant antibacterial properties to gauze and has the potential to be widely used for the antimicrobial treatment of clothes.
• Periodontal tissue regeneration by recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles.
  Yuto Yoshino; Hirofumi Miyaji; Erika Nishida; Yukimi Kanemoto; Asako Hamamoto; Akihito Kato; Tsutomu Sugaya; Tsukasa Akasaka
  Journal of oral biosciences, 17 Jan. 2023, [International Magazine]
  English, Scientific journal, OBJECTIVES: Recombinant human collagen peptide (RCP) is a recombinantly created xeno-free biomaterial enriched in arginine-glycine-aspartic acid sequences with good processability whose use for regenerative medicine applications is under investigation. The biocompatibility and osteogenic ability of RCP granules combined with β-tricalcium phosphate (TCP) submicron particles (β-TCP/RCP) were recently demonstrated. In the present study, β-TCP/RCP was implanted into experimental periodontal tissue defects created in beagles to investigate its regenerative effects. METHODS: An RCP solution was lyophilized, granulated, and thermally cross-linked into particles approximately 1 mm in diameter. β-TCP dispersion (1 wt%; 500 μL) was added to 100 mg of RCP granules to form β-TCP/RCP. A three-walled intrabony defect (5 mm × 3 mm × 4 mm) was created on the mesial side of the mandibular first molar and filled with β-TCP/RCP. RESULTS: A micro-computed tomography image analysis performed at 8 weeks postoperative showed a significantly greater amount of new bone after β-TCP/RCP grafting (2.2-fold, P<0.05) than after no grafting. Histological findings showed that the transplanted β-TCP/RCP induced active bone-like tissue formation including tartaric acid-resistant acid phosphatase- and OCN-positive cells as well as bioabsorbability. Ankylosis did not occur, and periostin-positive periodontal ligament-like tissue formation was observed. Histological measurements performed at 8 weeks postoperative revealed that β-TCP/RCP implantation formed 1.7-fold more bone-like tissue and 2.1-fold more periodontal ligament-like tissue than the control condition and significantly suppressed gingival recession and epithelial downgrowth (P<0.05). CONCLUSIONS: β-TCP/RCP implantation promoted bone-like and periodontal ligament-like tissue formation, suggesting its efficacy as a periodontal tissue regenerative material.
• Screening of heat-killed lactic acid bacteria based on inhibitory activity against oral bacteria and effects of oral administration of heat-killed Ligilactobacillus salivarius CP3365 on periodontal health in healthy participants: a double-blinded, randomized, placebo-controlled trial.
  Shinji Sakata; Yukiko Sakamaki; Masahiro Yuki; Tsutomu Sugaya; Tatsuhiko Hirota
  Journal of oral microbiology, 15, 1, 2250649, 2250649, 2023, [International Magazine]
  English, Scientific journal, OBJECTIVES: The aims of this study were to select heat-killed lactic acid bacteria (HKL) with antibiotic activity and investigate the efficacy of this bacteria in maintaining periodontal parameters in healthy participants. MATERIALS AND METHODS: An in vitro evaluation was conducted to assess the inhibitory efficacy of lactic acid bacteria against Porphyromonas gingivalis and Fusobacterium nucleatum subsp. nucleatum. The effects of HKL administration on various parameters (plaque control record, bleeding on probing, and probing pocket depth) were assessed in a randomized, placebo-controlled trial. Participants in the test and placebo groups (n = 32) consumed oral tablets containing placebo or HKL daily for 8 weeks. Oral bacteria in supra-plaque and saliva were identified using 16S rRNA gene community profiling analysis. RESULTS: Heat-killed Ligilactobacillus salivarius CP3365 significantly (p < 0.05) decreased the viability of oral bacteria and was selected for clinical trials. Administration of HKL CP3365 significantly (p < 0.05) inhibited increases in each parameter. No changes in the relative abundance of P. gingivalis or F. nucleatum subsp. nucleatum were detected by HKL CP3365, but the relative abundance of oral bacteria (genera Porphyromonas, Fusobacterium, and Haemophilus) was significantly (p < 0.05) decreased. CONCLUSION: HKL CP3365 effectively inhibited oral bacteria growth and was useful for maintaining periodontal health. CLINICAL TRIAL REGISTRATION: [https://www.umin.ac.jp/ctr/index.htm], identifier [UMIN000045656].
• Phosphorylated pullulan promotes calcification during bone regeneration in the bone defects of rat tibiae.
  Yasuhito Morimoto; Tomoka Hasegawa; Hiromi Hongo; Tomomaya Yamamoto; Haruhi Maruoka; Mai Haraguchi-Kitakamae; Ko Nakanishi; Tsuneyuki Yamamoto; Hotaka Ishizu; Tomohiro Shimizu; Kumiko Yoshihara; Yasuhiro Yoshida; Tsutomu Sugaya; Norio Amizuka
  Frontiers in bioengineering and biotechnology, 11, 1243951, 1243951, 2023, [International Magazine]
  English, Scientific journal, The current study aimed to evaluate bone tissue regeneration using a combination of β-tricalcium phosphate (βTCP) and phosphorylated pullulan (PPL, a phosphate-rich polysaccharide polymer consisting of maltotriose units). Round defects of 2 mm diameter were created in the arterial center of rat tibiae, which were further treated with vehicle (control group), βTCP (βTCP group), or βTCP + PPL (βTCP + PPL group) grafts. The control specimens without bone grafts exhibited rapid bone formation after 1 week; however, the regenerated bone was not resorbed until 4 weeks. In contrast, βTCP-grafted specimens exhibited fewer but thicker trabeculae, whereas the βTCP + PPL group displayed many fine trabeculae at 4 weeks. In the βTCP + PPL group, new bone was associated with the βTCP granules and PPL. Similarly, PHOSPHO1-positive osteoblasts were localized on the βTCP granules as well as the PPL. On the other hand, TRAP-reactive osteoclasts predominantly localized on newly-formed bone and βTCP granules rather than on the PPL. No significant differences were observed in the expression of Alp, Integrin αv, Osteopontin, Osteocalcin, and Dmp-1 in PPL-treated MC3T3-E1 osteoblastic cells, suggesting that PPL did not facilitate osteoblastic differentiation. However, von Kossa staining identified abundant needle-like calcified structures extending inside the PPL. Furthermore, transmission electron microscopy (TEM) revealed many globular structures identical to calcified nodules. In addition, calcified collagen fibrils were observed in the superficial layer of the PPL. Thus, PPL may serve as a scaffold for osteoblastic bone formation and promotes calcification on its surface. In conclusion, we speculated that βTCP and PPL might promote bone regeneration and could be integrated into promising osteoconductive materials.
• 壊死歯根膜を除去して再植し水酸化カルシウムを長期貼薬した置換性吸収歯の25年経過症例
  菅谷 勉
  日本外傷歯学会雑誌, 18, 1, 57, 62, (一社)日本外傷歯学会, Dec. 2022
  Japanese
• 酸化グラフェンとカオチン界面活性剤による透明超薄膜コーティングの持続的抗菌効果
  宮治 裕史; 金本 佑生実; 浜本 朝子; 蔀 佳奈子; 西田 絵利香; 加藤 昭人; 菅谷 勉; 田中 佐織; 相川 夏葉; 川崎 英也; 郷田 隼; 小野 博信
  日本バイオマテリアル学会大会予稿集, 44回, 394, 394, 日本バイオマテリアル学会, Nov. 2022
  Japanese
• Sustained antibacterial coating with graphene oxide ultrathin film combined with cationic surface-active agents in a wet environment
  Hirofumi Miyaji; Yukimi Kanemoto; Asako Hamamoto; Kanako Shitomi; Erika Nishida; Akihito Kato; Tsutomu Sugaya; Saori Tanaka; Natsuha Aikawa; Hideya Kawasaki; Syun Gohda; Hironobu Ono
  Scientific Reports, 12, 1, Springer Science and Business Media LLC, 18 Oct. 2022
  Scientific journal, Abstract
  
  Antimicrobial surfactants contained in mouthrinse have excellent efficacy, but are not retained on the tooth surface (are rinsed away) due to their low water resistance and thus do not exhibit sustained antibacterial activity. We have developed a new coating method using graphene oxide (GO) that retains the surfactant on the tooth surface even after rinsing with water, thus providing a sustained antibacterial effect. Ultra-thin films of GO and an antimicrobial agent were prepared by (1) applying GO to the substrate surface, drying, and thoroughly rinsing with water to remove excess GO to form an ultrathin film (almost a monolayer, transparent) on the substrate surface, then (2) applying antimicrobial cationic surface active agents (CSAAs) on the GO film to form a composite coating film (GO/CSAA). GO/CSAA formation was verified by scanning electron microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy, and ζ-potential and contact angle measurements. GO/CSAA was effective at inhibiting the growth of oral pathogens for up to 7 days of storage in water, and antibacterial activity was recovered by reapplication of the CSAA. Antibacterial GO/CSAA films were also formed on a tooth substrate. The results suggest that GO/CSAA coatings are effective in preventing oral infections.
• 光スタンプレーザー転写(LIFTOP)プロセスによる歯表面の改質
  宮治 裕史; 奈良崎 愛子; 大矢根 綾子; 菅谷 勉; 田中 佐織
  特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 157回, 182, 182, (NPO)日本歯科保存学会, Oct. 2022
  Japanese
• 全身性疾患への影響を考慮した新たな歯周病重症度検査項目の策定 学会主導型多施設臨床研究
  松田 真司; 菅谷 勉; 加藤 幸紀; 根本 英二; 竹内 康雄; 喜田 大智; 沼部 幸博; 西田 哲也; 小方 頼昌; 申 基哲; 長野 孝俊; 両角 俊哉; 小松 康高; 出分 菜々衣; 神谷 洋介; 北村 正博; 田口 洋一郎; 高柴 正悟; 湯本 浩通; 山下 明子; 吉永 泰周; 吉村 篤利; 河口 浩之
  日本歯周病学会会誌, 64, 秋季特別, 112, 112, (NPO)日本歯周病学会, Aug. 2022
  Japanese
• 前駆体利用バイオミメティックプロセスによる低結晶性アパタイト成膜足場材 骨・歯周組織形成能の評価
  金本 佑生実; 宮治 裕史; 西田 絵利香; 宮田 さほり; 眞弓 佳代子; 吉野 友都; 加藤 昭人; 菅谷 勉; 赤坂 司; 大矢根 綾子
  日本歯周病学会会誌, 64, 秋季特別, 122, 122, (NPO)日本歯周病学会, Aug. 2022
  Japanese
• Surface pre-reacted glass-ionomer(S-PRG)ナノフィラー分散液を注水に用いた超音波スケーラーによる歯周炎改善効果
  宮治 裕史; 眞弓 佳代子; 金本 佑生実; 岡本 一絵; 浜本 朝子; 加藤 昭人; 菅谷 勉; 赤坂 司; 田中 佐織
  日本歯周病学会会誌, 64, 秋季特別, 126, 126, (NPO)日本歯周病学会, Aug. 2022
  Japanese
• 全身性疾患への影響を考慮した新たな歯周病重症度検査項目の策定 学会主導型多施設臨床研究
  松田 真司; 菅谷 勉; 加藤 幸紀; 根本 英二; 竹内 康雄; 喜田 大智; 沼部 幸博; 西田 哲也; 小方 頼昌; 申 基哲; 長野 孝俊; 両角 俊哉; 小松 康高; 出分 菜々衣; 神谷 洋介; 北村 正博; 田口 洋一郎; 高柴 正悟; 湯本 浩通; 山下 明子; 吉永 泰周; 吉村 篤利; 河口 浩之
  日本歯周病学会会誌, 64, 秋季特別, 112, 112, (NPO)日本歯周病学会, Aug. 2022
  Japanese
• Surface pre-reacted glass-ionomer(S-PRG)フィラー配合水硬性仮封材の抗菌特性
  宮治 裕史; 金本 佑生実; 菅谷 勉; 田中 佐織
  日本歯内療法学会学術大会プログラム・抄録集, 43回, 61, 61, (一社)日本歯内療法学会, Jul. 2022
  Japanese
• Ultrasonic irrigation of periodontal pocket with surface pre-reacted glass-ionomer (S-PRG) nanofiller dispersion improves periodontal parameters in beagle dogs.
  Hirofumi Miyaji; Kayoko Mayumi; Yukimi Kanemoto; Ichie Okamoto; Asako Hamamoto; Akihito Kato; Tsutomu Sugaya; Tsukasa Akasaka; Saori Tanaka
  Journal of oral biosciences, 64, 2, 222, 228, Jun. 2022, [International Magazine]
  English, Scientific journal, OBJECTIVES: Surface pre-reacted glass-ionomer (S-PRG) nanofiller, an antibacterial ion-releasing bioactive glass, has been shown to adhere to tooth surfaces and reported to improve inflammatory parameters in experimental periodontitis. In this study, cementum substrate was irrigated ultrasonically with dispersion to examine in-vitro nanofiller adhesion and antibacterial activity. Moreover, periodontal pockets in a beagle dog were ultrasonically irrigated with dispersion to assess periodontal healing. METHODS: The morphology of human cementum irrigated with S-PRG nanofiller dispersion was examined by scanning electron microscopy and energy dispersive X-ray spectrometry. The antibacterial activity of the treated cementum was tested using Actinomyces naeslundii. In addition, experimentally formed periodontal pockets in beagle dog were ultrasonically irrigated with S-PRG nanofiller dispersion. Periodontal parameters (gingival index, bleeding on probing, probing pocket depth, and clinical attachment level) were measured from baseline (0 weeks) through 12 weeks. Moreover, the effects of irrigation with S-PRG nanofiller on changes in periodontal microflora and bone healing were analyzed. RESULTS: After ultrasonic irrigation, S-PRG nanofiller adhered to the cementum and exhibited antibacterial activity. The periodontal parameters were shown to improve following ultrasonic irrigation with S-PRG nanofiller dispersion. Analysis by next-generation sequencing revealed that the ratio of red-complex species decreased in the pockets irrigated with S-PRG nanofiller dispersion. In addition, the S-PRG nanofiller showed the potential to promote bone healing. CONCLUSIONS: Ultrasonic irrigation with S-PRG nanofiller dispersion using an ultrasonic scaler system permitted delivery of the S-PRG nanofiller to the root surface, providing improved parameters in experimental periodontitis and modifying the composition of subgingival periodontal microflora.
• 酸化グラフェン超薄膜コーティングへの口腔洗浄含嗽剤の複合化による持続性抗菌効果
  宮治 裕史; 金本 佑生実; 浜本 朝子; 西田 絵利香; 加藤 昭人; 菅谷 勉; 田中 佐織; 郷田 隼; 小野 博信
  特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 156回, 89, 89, (NPO)日本歯科保存学会, May 2022
  Japanese
• 歯ブラシ・歯磨剤・洗口剤の理解を深めるための体感型授業
  田中 佐織; 菅谷 勉; 西川 昌弘
  日本歯周病学会会誌, 64, 春季特別, 124, 124, (NPO)日本歯周病学会, May 2022
  Japanese
• In Vivo Inflammatory Effects and Surface Composition Changes in Implanted Root Canal Sealer Containing Bioactive Glass
  Akihito KATO; Hirofumi MIYAJI; Yuto YOSHINO; Yukimi KANEMOTO; Asako HAMAMOTO; Erika NISHIDA; Tsutomu SUGAYA; Saori TANAKA
  The Japanese Journal of Conservative Dentistry, 65, 2, 145, 153, The Japanese Society of Conservative Dentistry, 30 Apr. 2022
  Japanese
• Application of simple stress reduction methods for safe and secure periodontal treatment
  Shinji Shimoji; Hiroe Takefu; Tsutomu Sugaya
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 64, 1, 9, 16, Japanese Society of Periodontology, 31 Mar. 2022
  Scientific journal
• Electro-Magnetic Apical Treatment(EMAT) 高周波通電を応用した難治性根尖歯周炎への対応
  富永 敏彦; 高比良 一輝; 多田 瑛一朗; 菅谷 勉
  日本歯内療法学会雑誌, 43, 1, 28, 36, (一社)日本歯内療法学会, Jan. 2022
  Japanese, 症例1は61歳男性で、主訴は上顎左側側切歯部の違和感、補綴物の脱離で、臨床診断は上顎左側側切歯、慢性根尖性歯周炎であった。2分間passive ultrasonic irrigation(PUI)を行い、水酸化カルシウム製剤を貼薬し、仮封を行った。その後、違和感は消失していたものの、根尖部の肉芽組織に変化は認められず、根尖孔からの出血がみられたため、局所麻酔下で高周波通電(HFC)を行った。HFC後、根尖部の肉芽組織は瞬時に白色に変性し止血した。4回目の治療では症状はなく、根尖部に白色の硬い層がみられ、その下層には、淡桃色で半透明の弾力性のある肉芽組織が認められた。HFC 2ヵ月後のCBCT画像では、X線透過像は術前と比較して縮小しており、8ヵ月後にはほぼ消失していた。症例2は71歳女性で、主訴は上顎左側中切歯部の自発痛、膨張、咀嚼時痛で、臨床診断は上顎左側中切歯、急性根尖性歯周炎であった。ビタペックス貼薬等の治療を行ったが、根尖孔から易出血性の炎症性肉芽組織が根管内に侵入し、出血は増悪していた。電極を根尖孔部、歯根外表面および根尖病変内に位置させて、それぞれ2、4、2回HFCを行った。その後、穿孔部のビタペックスを除去したところ、炎症性肉芽組織が認められたため、穿孔部に2回HFCを行った。HFC後、根尖孔部の出血は軽減し、穿孔部には変性した組織が認められ、止血が確認できた。PUIを行い、ビタペックスを貼薬した。HFC 9ヵ月には歯根周囲にわずかなX線透過像が認められるものの唇側骨の再生が認められ、経過は良好に推移している。
• 壊死歯根膜を除去して再植し水酸化カルシウムを長期貼薬した置換性吸収歯の25年経過症例
  菅谷 勉
  日外傷歯誌, 18, 57, 62, 2022, [Peer-reviewed], [Last author]
  Japanese, Scientific journal
• Effects of BMP-2 on bone response using gelatin hydrogel in estrogen-deficient rats
  Yamaji K; Yokoyama A; Matsuzaki K; Sugaya T; Shimada Y; Ohara N; Yoshiyama M
  J Oral Tissue Engin, 20, 2, 41, 46, 2022, [Peer-reviewed]
  English, Scientific journal
• Fabrication and cell behaivior assessment of antibacterial micro/nano-patterned chitosan film
  Nishida E; Miyaji H; Sugaya T; Akasaka T
  Nano Biomed, 14, 1, 1, 8, 2022
• 歯周病ポケット検査における代表歯・部位の選定
  両角 俊哉; 野村 義明; 中川 種昭; 沼部 幸博; 菅谷 勉; 佐藤 聡; 野口 和行; 吉成 伸夫; 吉村 篤利; 西村 英紀; 齋藤 淳; 福田 光男; 小林 宏明; 菅野 直之; 多部田 康一; 高橋 慶壮; 梅田 誠; 高柴 正悟; 三邉 正人; 小方 頼昌
  神奈川歯学, 56, Abstracts, P, 39, 神奈川歯科大学学会, Dec. 2021
  Japanese
• Periodontal tissue engineering using an apatite/collagen scaffold obtained by a plasma- and precursor-assisted biomimetic process.
  Yukimi Kanemoto; Hirofumi Miyaji; Erika Nishida; Saori Miyata; Kayoko Mayumi; Yuto Yoshino; Akihito Kato; Tsutomu Sugaya; Tsukasa Akasaka; Arputharaj Joseph Nathanael; Syama Santhakumar; Ayako Oyane
  Journal of periodontal research, 57, 1, 205, 218, 16 Nov. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND AND OBJECTIVES: In the treatment of severe periodontal destruction, there is a strong demand for advanced scaffolds that can regenerate periodontal tissues with adequate quality and quantity. Recently, we developed a plasma- and precursor-assisted biomimetic process by which a porous collagen scaffold (CS) could be coated with low-crystalline apatite. The apatite-coated collagen scaffold (Ap-CS) promotes cellular ingrowth within the scaffold compared to CS in rat subcutaneous tissue. In the present study, the osteogenic activity of Ap-CS was characterized by cell culture and rat skull augmentation tests. In addition, the periodontal tissue reconstruction with Ap-CS in a beagle dog was compared to that with CS. METHODS: The plasma- and precursor-assisted biomimetic process was applied to CS to obtain Ap-CS with a low-crystalline apatite coating. The effects of apatite coating on the scaffold characteristics (i.e., surface morphology, water absorption, Ca release, protein adsorption, and enzymatic degradation resistance) were assessed. Cyto-compatibility and the osteogenic properties of Ap-CS and CS were assessed in vitro using preosteoblastic MC3T3-E1 cells. In addition, we performed in vivo studies to evaluate bone augmentation and periodontal tissue reconstruction with Ap-CS and CS in a rat skull and canine furcation lesion, respectively. RESULTS: As previously reported, the plasma- and precursor-assisted biomimetic process generated a low-crystalline apatite layer with a nanoporous structure that uniformly covered the Ap-CS surface. Ap-CS showed significantly higher water absorption, Ca release, lysozyme adsorption, and collagenase resistance than CS. Cell culture experiments revealed that Ap-CS was superior to CS in promoting the osteoblastic differentiation of MC3T3-E1 cells while suppressing their proliferation. Additionally, Ap-CS significantly promoted (compared to CS) the augmentation of the rat skull bone and showed the potential to regenerate alveolar bone in a dog furcation defect. CONCLUSION: Ap-CS fabricated by the plasma- and precursor-assisted biomimetic process provided superior promotion of osteogenic differentiation and bone neoformation compared to CS.
• 骨補填材としてのリン酸化プルラン/β-TCPコンビネーションマテリアルの応用
  森本 康仁; 久保田 恵亮; 阿部 未来; 丸岡 春日; 長谷川 智香; 本郷 裕美; 吉田 靖弘; 網塚 憲生; 菅谷 勉
  日本歯周病学会会誌, 63, 秋季特別, 120, 120, (NPO)日本歯周病学会, Oct. 2021
  Japanese
• リゾチーム-金ナノクラスター/ローズベンガル複合体の抗菌・抗バイオフィルム光線力学的活性
  岡本 一絵; 宮治 裕史; 宮田 さほり; 菅谷 勉; 蔀 佳奈子; 赤坂 司; 齋田 慧; 川崎 英也
  日本歯周病学会会誌, 63, 秋季特別, 133, 133, (NPO)日本歯周病学会, Oct. 2021
  Japanese
• S-PRGナノフィラーを用いた抗菌性歯面コーティングによる臨床パラメーターの改善 イヌ実験的歯周炎における検討
  眞弓 佳代子; 宮治 裕史; 宮田 さほり; 西田 絵利香; 降旗 友和; 金本 佑生実; 菅谷 勉; 蔀 佳奈子; 赤坂 司
  日本歯周病学会会誌, 63, 秋季特別, 133, 133, (NPO)日本歯周病学会, Oct. 2021
  Japanese
• 酸化グラフェン/塩化セチルピリジニウム複合超薄膜による歯面コーティングと抗菌評価
  宮治 裕史; 浜本 朝子; 西田 絵利香; 菅谷 勉; 蔀 佳奈子; 郷田 隼; 小野 博信
  日本歯周病学会会誌, 63, 秋季特別, 136, 136, (NPO)日本歯周病学会, Oct. 2021
  Japanese
• 根管拡大形成を簡略化する高周波通電と接着根管充填
  菅谷 勉
  北海道歯学雑誌, 42, 2, 7, 北海道歯学会, Sep. 2021
  Japanese
• リン酸化プルラン/β-TCPコンビネーションマテリアルにおける新規骨再生誘導
  森本 康仁; 久保田 恵亮; 阿部 未来; 丸岡 春日; 本郷 裕美; 吉田 靖弘; 菅谷 勉; 網塚 憲生; 長谷川 智香
  日本骨形態計測学会雑誌, 31, 2, S135, S135, 日本骨形態計測学会, Jun. 2021
  Japanese
• Application of high-frequency conduction for persistent apcal periodontitis: A case report
  Tominga T; Tada E; Takahira K; Sugaya T
  J Dent & Oral Disord, 7, 4, 1168, Jun. 2021, [Peer-reviewed], [Last author]
  English
• Antibacterial and Antibiofilm Photodynamic Activities of Lysozyme-Au Nanoclusters/Rose Bengal Conjugates
  Ichie Okamoto; Hirofumi Miyaji; Saori Miyata; Kanako Shitomi; Tsutomu Sugaya; Natsumi Ushijima; Tsukasa Akasaka; Satoshi Enya; Satoshi Saita; Hideya Kawasaki
  ACS Omega, 6, 13, 9279, 9290, American Chemical Society (ACS), 06 Apr. 2021, [International Magazine]
  English, Scientific journal, Antibacterial photodynamic therapy (aPDT) utilizes reactive oxygen species such as singlet oxygen (1O2) and free radicals via photosensitizers, which are light and light-sensitive agents, to reduce bacterial infections. It has been utilized as a treatment for dental diseases in place of antibiotic therapies. However, aPDT does not always cause the desired therapeutic effect due to the instability of organic photosensitizers and the formation of bacterial biofilms. To promote the antibacterial and antibiofilm effects of aPDT, we have proposed a lysozyme (Lys)-gold nanoclusters (Au NCs)/rose bengal (Lys-Au NCs/RB) conjugate as a novel photosensitizer. This conjugate was found to effectively impede the growth of both gram-positive and gram-negative bacteria when exposed to white light-emitting diode (LED) irradiation. The photoexcited Lys-Au NCs/RB showed significantly higher antibacterial activity than photoexcited Lys-Au NCs or RB alone. The synergistic effect is a result of the combination of Lys (an antibacterial protein) and enhanced 1O2 generation related to resonance energy transfer (RET) in the Au NCs/RB conjugate. Photoexcited Lys-Au NCs/RB increased the effects of aPDT in a dose- and time-dependent manner. Furthermore, the photoexcited Lys-Au NCs/RB successfully decreased Streptococcus mutans biofilm formation. However, in contrast, it did not have a negative effect on the proliferation, adhesion, or spread of mammalian cells, indicating low cytotoxicity. Lys-Au NCs/RB is a novel photosensitizer with low cytotoxicity that is capable of bacterial inactivation and the suppression of biofilm formation, and could help to improve dental treatments in the future.
• Prospective Longitudinal Changes in the Periodontal Inflamed Surface Area Following Active Periodontal Treatment for Chronic Periodontitis.
  Yoshiaki Nomura; Toshiya Morozumi; Atsushi Saito; Atsutoshi Yoshimura; Erika Kakuta; Fumihiko Suzuki; Fusanori Nishimura; Hideki Takai; Hiroaki Kobayashi; Kazuyuki Noguchi; Keiso Takahashi; Koichi Tabeta; Makoto Umeda; Masato Minabe; Mitsuo Fukuda; Naoyuki Sugano; Nobuhiro Hanada; Nobuo Yoshinari; Satoshi Sekino; Shogo Takashiba; Soh Sato; Toshiaki Nakamura; Tsutomu Sugaya; Yohei Nakayama; Yorimasa Ogata; Yukihiro Numabe; Taneaki Nakagawa
  Journal of clinical medicine, 10, 6, 1165, 1165, MDPI AG, 10 Mar. 2021, [International Magazine]
  English, Scientific journal, Periodontal disease is a chronic inflammatory disease of the periodontal tissue. The periodontal inflamed surface area (PISA) is a proposed index for quantifying the inflammatory burden resulting from periodontitis lesions. This study aimed to investigate longitudinal changes in the periodontal status as evaluated by the PISA following the active periodontal treatment. To elucidate the prognostic factors of PISA, mixed-effect modeling was performed for clinical parameters, tooth-type, and levels of periodontal pathogens as independent variables. One-hundred-twenty-five patients with chronic periodontitis who completed the active periodontal treatment were followed-up for 24 months, with evaluations conducted at 6-month intervals. Five-times repeated measures of mean PISA values were 130+/−173, 161+/−276, 184+/−320, 175+/−417, and 209+/−469 mm2. Changes in clinical parameters and salivary and subgingival periodontal pathogens were analyzed by mixed-effect modeling. Plaque index, clinical attachment level, and salivary levels of Porphyromonas gingivalis were associated with changes in PISA at the patient- and tooth-level. Subgingival levels of P. gingivalis and Prevotella intermedia were associated with changes in PISA at the sample site. For most patients, changes in PISA were within 10% of baseline during the 24-month follow-up. However, an increase in the number of bleeding sites in a tooth with a deep periodontal pocket increased the PISA value exponentially.
• Estimation of the Periodontal Inflamed Surface Area by Simple Oral Examination.
  Yoshiaki Nomura; Toshiya Morozumi; Yukihiro Numabe; Yorimasa Ogata; Yohei Nakayama; Tsutomu Sugaya; Toshiaki Nakamura; Soh Sato; Shogo Takashiba; Satoshi Sekino; Nobuo Yoshinari; Nobuhiro Hanada; Naoyuki Sugano; Mitsuo Fukuda; Masato Minabe; Makoto Umeda; Koichi Tabeta; Keiso Takahashi; Kazuyuki Noguchi; Hiroaki Kobayashi; Hideki Takai; Fusanori Nishimura; Fumihiko Suzuki; Erika Kakuta; Atsutoshi Yoshimura; Atsushi Saito; Taneaki Nakagawa
  Journal of clinical medicine, 10, 4, 723, 723, MDPI AG, 12 Feb. 2021, [International Magazine]
  English, Scientific journal, The periodontal inflamed surface area (PISA) is a useful index for clinical and epidemiological assessments, since it can represent the inflammation status of patients in one contentious variable. However, calculation of the PISA is difficult, requiring six point probing depth measurements with or without bleeding on probing on 28 teeth, followed by data input in a calculation program. More simple methods are essential for screening periodontal disease or in epidemiological studies. In this study, we tried to establish a convenient partial examination method to estimate PISA. Cross-sectional data of 254 subjects who completed active periodontal therapy were analyzed. Teeth that represent the PISA value were selected by an item response theory approach. The maxillary second molar, first premolar, and lateral incisor and the mandibular second molar and lateral incisor were selected. The sum of the PISAs of these teeth was significantly correlated with the patient’s PISA (R2 = 0.938). More simply, the sum of the maximum values of probing pocket depth with bleeding for these teeth were also significantly correlated with the patient’s PISA (R2 = 0.6457). The simple model presented in this study may be useful to estimate PISA.
• 機械的根管拡大形成の限界を超える高周波電流による歯内療法(Part2) ファイル未到達根管での発熱と放電を応用した根管治療
  菅谷 勉; 富永 敏彦; 鷲巣 太郎; 西田 絵利香; 熊谷 広道
  The Quintessence, 40, 2, 0458, 0467, クインテッセンス出版(株), Feb. 2021
  Japanese
• Antibacterial coating of tooth surface with ion-releasing pre-reacted glass-ionomer (S-PRG) nanofillers
  Kayoko Mayumi; Hirofumi Miyaji; Saori Miyata; Erika Nishida; Tomokazu Furihata; Yukimi Kanemoto; Tsutomu Sugaya; Kanako Shitomi; Tsukasa Akasaka
  Heliyon, 7, 2, e06147, e06147, Elsevier BV, Feb. 2021
  English, Scientific journal
• Evaluation of antibacterial and cytocompatible properties of multiple-ion releasing zinc-fluoride glass nanoparticles
  Erika NISHIDA; Hirofumi MIYAJI; Kanako SHITOMI; Tsutomu SUGAYA; Tsukasa AKASAKA
  Dental Materials Journal, 40, 1, 157, 164, Japanese Society for Dental Materials and Devices, 25 Jan. 2021, [Domestic magazines]
  English, Scientific journal, Zinc-fluoride glass nanoparticles (Zinc-F) release several ions, such as fluoride, zinc and calcium ions, through acid-base reactions. The aim of this study was to evaluate the antibacterial and cytotoxic properties of Zinc-F. Antibacterial tests showed that a Zinc-F eluting solution significantly reduced the turbidity and colony-forming units of Streptococcus mutans and Actinomyces naeslundii, compared to that of calcium-fluoroaluminosilicate glass nanoparticles without zinc ions. In live/dead staining, Zinc-F eluate significantly decreased green-stained bacterial cells, indicating live cells, compared with the control (no application). Human dentin coated with Zinc-F showed suppressed S. mutans and A. naeslundii biofilm formation. Additionally, Zinc-F eluate showed low cytotoxic effects in osteoblastic and fibroblastic cells. Therefore, our findings suggested that Zinc-F exhibits antibacterial and biocompatible properties through multiple-ion release.
• Optimal Examination Sites for Periodontal Disease Evaluation: Applying the Item Response Theory Graded Response Model
  Yoshiaki Nomura; Toshiya Morozumi; Mitsuo Fukuda; Nobuhiro Hanada; Erika Kakuta; Hiroaki Kobayashi; Masato Minabe; Toshiaki Nakamura; Yohei Nakayama; Fusanori Nishimura; Kazuyuki Noguchi; Yukihiro Numabe; Yorimasa Ogata; Atsushi Saito; Soh Sato; Satoshi Sekino; Naoyuki Sugano; Tsutomu Sugaya; Fumihiko Suzuki; Keiso Takahashi; Hideki Takai; Shogo Takashiba; Makoto Umeda; Hiromasa Yoshie; Atsutoshi Yoshimura; Nobuo Yoshinari; Taneaki Nakagawa
  Journal of Clinical Medicine, 9, 11, 3754, 3754, MDPI AG, 21 Nov. 2020, [International Magazine]
  English, Scientific journal, Periodontal examination data have a complex structure. For epidemiological studies, mass screenings, and public health use, a simple index that represents the periodontal condition is necessary. Periodontal indices for partial examination of selected teeth have been developed. However, the selected teeth vary between indices, and a justification for the selection of examination teeth has not been presented. We applied a graded response model based on the item response theory to select optimal examination teeth and sites that represent periodontal conditions. Data were obtained from 254 patients who participated in a multicenter follow-up study. Baseline data were obtained from initial follow-up. Optimal examination sites were selected using item information calculated by graded response modeling. Twelve sites—maxillary 2nd premolar (palatal-medial), 1st premolar (palatal-distal), canine (palatal-medial), lateral incisor (palatal-central), central incisor (palatal-distal) and mandibular 1st premolar (lingual, medial)—were selected. Mean values for clinical attachment level, probing pocket depth, and bleeding on probing by full mouth examinations were used for objective variables. Measuring the clinical parameters of these sites can predict the results of full mouth examination. For calculating the periodontal index by partial oral examination, a justification for the selection of examination sites is essential. This study presents an evidence-based partial examination methodology and its modeling.
• キャナルスペーストのユージノール放出量の検討
  宮治 裕史; 金本 佑生実; 菅谷 勉; 蔀 佳奈子; 田中 佐織
  特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 153回, 72, 72, (NPO)日本歯科保存学会, Nov. 2020
  Japanese
• Bone forming ability of recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles.
  Tomokazu Furihata; Hirofumi Miyaji; Erika Nishida; Akihito Kato; Saori Miyata; Kanako Shitomi; Kayoko Mayumi; Yukimi Kanemoto; Tsutomu Sugaya; Tsukasa Akasaka
  Journal of biomedical materials research. Part B, Applied biomaterials, 108, 7, 3033, 3044, Oct. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• リン酸化プルラン含有根管充填シーラーの封鎖性と根尖病巣の治療効果
  大嶌 理紗; 河野 通俊; 下地 伸司; 菅谷 勉; 吉田 靖弘
  北海道外科雑誌, 65, 1, 91, 92, 北海道外科学会, Jun. 2020
  Japanese
• 過飽和液中レーザー照射法による象牙質表面へのフッ素担持アパタイト成膜と抗菌特性
  蔀 佳奈子; 宮治 裕史; 菅谷 勉; 古賀 健司; 中村 真紀; 大矢根 綾子
  特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 152回, 80, 80, (NPO)日本歯科保存学会, Jun. 2020
  Japanese
• Bioactive Glass配合根管充填シーラーの生体親和性評価
  吉野 友都; 宮治 裕史; 金本 佑生実; 浜本 朝子; 西田 絵利香; 加藤 昭人; 菅谷 勉; 田中 佐織
  特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 152回, 103, 103, (NPO)日本歯科保存学会, Jun. 2020
  Japanese
• Heat generation and temperature increase in the root during electromagnetic apical treatment.
  Tominaga T; Tada E; Takahira K; Sugaya T
  6, 1, Jun. 2020, [Peer-reviewed], [Last author]
  English
• Photodynamic inactivation of oral bacteria with silver nanoclusters/rose bengal nanocomposite.
  Kanako Shitomi; Hirofumi Miyaji; Saori Miyata; Tsutomu Sugaya; Natsumi Ushijima; Tsukasa Akasaka; Hideya Kawasaki
  Photodiagnosis and photodynamic therapy, 30, 101647, 101647, Jun. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Antimicrobial photodynamic therapy (a-PDT) is a promising anti-infective technique for generation of singlet oxygen (1O2) to target dental disease. However, conventional organic photosensitizers have problems for clinical use in terms of cytotoxicity, quenching of a-PDT activity by self-dimerization, and the lack of long-term antibacterial effect. We herein propose silver nanoclusters/rose bengal nanocomposite (AgNCs/RB) as a novel photosensitizer with two primary antibacterial effects: (1) 1O2 generation by irradiated RB and (2) Ag+ ion release from AgNCs. AgNCs/RB irradiated with white light-emitting diode (LED) for a short irradiation time of 1 min significantly decreased the bacterial turbidity of Streptococcus mutans, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans (P < 0.05). In SEM, TEM and LIVE/DEAD staining images, photoexcited AgNCs/RB reduced S. mutans colonization, destroyed the cell membrane, and increased the number of dead cells. The antibacterial efficiency of photoexcited AgNCs/RB was greater than that of AgNCs or RB alone (P < 0.05), suggesting a synergistic effect of 1O2 and Ag+ ions from photoexcited AgNCs/RB. By contrast, photoexcited AgNCs/RB did not affect WST-8 and LDH activities and morphology of NIH3T3 mammalian cells, indicating low cytotoxicity. Interestingly, the antibacterial activity of AgNCs/RB on S. mutans was maintained even after the cessation of LED irradiation, indicating a long-term antibacterial effect due to released Ag+ ions. The present AgNCs/RB photosensitizers provide effective synergistic antibacterial effects for dental a-PDT via 1O2 and Ag+ ions coupled with low cytotoxicity.
• The Clinical Efficacy of an Ultrasonic Device with Built-In Electronic Apex Locator when Preparing Vertical Root Fracture Lines Through the Root Canal
  M Kudou; Y Motoki; K Inoue; S Tanaka; H Miyaji; M Kawanami; T Sugaya
  Dentistry, 9, 8, 549, Jan. 2020, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal
• ヒトI型コラーゲン様リコンビナントペプチド/サブミクロンβ-TCP骨補填材による骨形成効果
  西田 絵利香; 宮治 裕史; 降籏 友和; 加藤 昭人; 宮田 さほり; 蔀 佳奈子; 眞弓 佳代子; 金本 佑生実; 菅谷 勉; 赤坂 司
  日本バイオマテリアル学会大会予稿集, 41回, 369, 369, 日本バイオマテリアル学会, Nov. 2019
  Japanese
• Human dentin applied with silver nanoclusters exhibited the antibacterial activity on Streptococcus mutans
  K Shitomi; H Miyaji; S Miyata; E Nishida; K Mayumi; T Sugaya; H Kawasaki
  Nano Biomed, 11, 1, 121, 28, Aug. 2019, [Peer-reviewed]
  English, Scientific journal
• 銀ナノクラスターで被覆したヒト象牙質はStreptococcus mutansに対する抗菌活性を示す(Human Dentin Coated with Silver Nanoclusters Exhibits Antibacterial Activity against Streptococcus mutans)
  Shitomi Kanako; Miyaji Hirofumi; Miyata Saori; Nishida Erika; Mayumi Kayoko; Sugaya Tsutomu; Kawasaki Hideya
  Nano Biomedicine, 11, 1, 21, 28, ナノ・バイオメディカル学会, Jun. 2019, [Peer-reviewed]
  English, 虫歯の病原菌であるStreptococcus mutansに対して審美的(象牙質変色予防)作用と抗菌的作用を持つ成分として、直径2nm未満の銀ナノクラスター(AgNC)を調製した。このAgNCはS.mutansの濁度と生存性を顕著に減少させるだけでなく、LIVE/DEAD染色法にて殺菌効果が認められた。このAgNCをヒト象牙質に適用すると、フッ化ジアミン銀を適用した場合と比べて象牙質の変色は認められず、AgNC-処理象牙質はS.mutansによるコロニー形成に対する阻害効果を示した。これらの結果から、AgNCは抗菌的・審美的基質として、歯科療法で有望と考えられた。
• Effects of transforming growth factor-β1 and bone morphogenetic protein-2 on bone formation using a bioabsorbable scaffold at palatal sites in rats
  J Oral Tissue Engin, 2019, [Peer-reviewed]
• Effect of gelatin;hydrogel containing BMP;on bone;formation in aged rats
  Yamaji K; Yokoyama A; Matsuzaki K; Ohara N; Sugaya T; Yoshiyama M
  J Oral Engin, 19, 1, 10, 14, Jan. 2019, [Peer-reviewed]
  English, Scientific journal
• Prolonged release of bone morphogenetic protein-2 in vivo by gene transfection with DNA-functionalized calcium phosphate nanoparticle-loaded collagen scaffolds.
  Taichi Tenkumo; Juan Ramón Vanegas Sáenz; Keisuke Nakamura; Yoshinaka Shimizu; Viktoriya Sokolova; Matthias Epple; Yuya Kamano; Hiroshi Egusa; Tsutomu Sugaya; Keiichi Sasaki
  Materials science & engineering. C, Materials for biological applications, 92, 172, 183, 01 Nov. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, In the combination of scaffolds immersed in growth factor solutions, the release of growth factors mainly depends on scaffold degradation. However, the release of bone morphogenetic protein (BMP)-2 at an appropriate concentration during the stage of tissue regeneration would enhance bone regeneration. To achieve this condition, the present study was performed to investigate the effects of scaffolds combined with gene transfection using non-viral vectors. Nanohydroxyapatite-collagen (nHAC) scaffolds cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) or ascorbic acid/copper chloride, and a collagen scaffold (Terdermis®) were prepared, loaded with BMP-2-encoding plasmid DNA-functionalized calcium phosphate nanoparticles (CaP), naked plasmid DNA, or BMP-2 solution, and implanted in rats. The yield of released BMP-2 and its releasing period, respectively, were larger and longer from the scaffolds loaded with CaP than from those incubated with BMP-2 solution. In addition, the alkaline phosphatase activity induced by the CaP-loaded scaffolds was higher. Histological analysis showed that released BMP-2 could be observed on the macrophages or multinuclear giant cells surrounding the nHAC fragments or collagen fibres. TRAP-positive or OCN-positive sites were observed in all groups and a mineralization area was observed in the Terdermis®/CaP sample. The present study demonstrates that gene transfection by scaffold loaded with CaP gene transfer vectors induces a larger yield of BMP-2 for a longer period than by scaffolds loaded with BMP-2 solution or naked plasmid.
• 健全な若年成人における歯周基本治療時のアロマテラピーの効果 心拍変動解析を用いた自律神経活動評価
  下地 伸司; 竹生 寛恵; 大嶌 理紗; 川浪 雅光; 菅谷 勉
  日本歯科保存学雑誌, 61, 5, 282, 291, (NPO)日本歯科保存学会, Oct. 2018
  Japanese
• Near-infrared Irradiation and Graphene Oxide Film Fabricated on Dentin Surface Exhibit Photothermal and Antibacterial Effects
  Nagao Keishi; Miyaji Hirofumi; Nishida Erika; Akasaka Tsukasa; Miyata Saori; Shitomi Kanako; Mayumi Kayoko; Kato Akihito; Sugaya Tsutomu
  Journal of Oral Hygiene & Health, 6, 1, 231, 231, OMICS International, 10 Jan. 2018, [Peer-reviewed]
  English, Scientific journal
• Preparation of micro/nanopatterned gelatins crosslinked with genipin for biocompatible dental implants.
  Reika Makita; Tsukasa Akasaka; Seiichi Tamagawa; Yasuhiro Yoshida; Saori Miyata; Hirofumi Miyaji; Tsutomu Sugaya
  Beilstein journal of nanotechnology, 9, 1735, 1754, 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Background: Collagen is a basic component of the periodontium and plays an important role in the function of the periodontal unit. Therefore, coating with collagen/gelatin has been applied to enable dental implants to positively interact with peri-implant tissues. Although the micro/nanoscale topography is an important property of the surface of dental implants, smaller collagen/gelatin surface patterns have not been sufficiently developed. Furthermore, only few reports on the behavior of cells on gelatin surfaces with different patterns and sizes exist. In this study, we developed micro/nanometer-scaled gelatin surfaces using genipin crosslinking, with the aim of understanding the use of patterning in surface modification of dental implants. Results: Grooves, holes, and pillars, with widths or diameters of 2 µm, 1 µm, or 500 nm were fabricated using a combination of molding and genipin crosslinking of gelatin. The stability of the different gelatin patterns could be controlled by the degree of genipin crosslinking. The gelatin patterns at 20 mM concentration of genipin and 41% crosslinking maintained a stable, patterned shape for at least 14 days in a cell culture medium. A cell morphology study showed that the cells on groves were aligned along the direction of the grooves. In contrast, the cells on pillars and holes exhibited randomly elongated filopodia. The vinculin spots of the cells were observed on the top of ridges and pillars or the upper surface of holes. The results of a cell attachment assay showed that the number of surface-attached cells increased with increasing patterning of the gelatin surface. Unlike the cell attachment assay, the results of a cell proliferation assay showed that Saos-2 cells prefer grooves with diameters of approximately 2 µm and 1 µm and pillars with diameters of 1 µm and heights of 500 nm. The number of cells on pillars with heights of 2 µm was larger than those of the other gelatin surface patterns tested. Conclusion: These data support that a detailed design of the gelatin surface pattern can control both cell attachment and proliferation of Saos-2 cells. Thus, gelatin surfaces patterned using genipin crosslinking are now an available option for biocompatible material patterning.
• Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog.
  Kohei Kawamoto; Hirofumi Miyaji; Erika Nishida; Saori Miyata; Akihito Kato; Akito Tateyama; Tomokazu Furihata; Kanako Shitomi; Toshihiko Iwanaga; Tsutomu Sugaya
  International journal of nanomedicine, 13, 2365, 2376, 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Introduction: The 3-dimensional scaffold plays a key role in volume and quality of repair tissue in periodontal tissue engineering therapy. We fabricated a novel 3D collagen scaffold containing carbon-based 2-dimensional layered material, named graphene oxide (GO). The aim of this study was to characterize and assess GO scaffold for periodontal tissue healing of class II furcation defects in dog. Materials and methods: GO scaffolds were prepared by coating the surface of a 3D collagen sponge scaffold with GO dispersion. Scaffolds were characterized using cytotoxicity and tissue reactivity tests. In addition, GO scaffold was implanted into dog class II furcation defects and periodontal healing was investigated at 4 weeks postsurgery. Results: GO scaffold exhibited low cytotoxicity and enhanced cellular ingrowth behavior and rat bone forming ability. In addition, GO scaffold stimulated healing of dog class II furcation defects. Periodontal attachment formation, including alveolar bone, periodontal ligament-like tissue, and cementum-like tissue, was significantly increased by GO scaffold implantation, compared with untreated scaffold. Conclusion: The results suggest that GO scaffold is biocompatible and possesses excellent bone and periodontal tissue formation ability. Therefore, GO scaffold would be beneficial for periodontal tissue engineering therapy.
• Evaluation of tissue behavior on three-dimensional collagen scaffold coated with carbon nanotubes and β-tricalcium phosphate nanoparticles
  J Oral Tissue Engin, 15, 3, 123, 130, 2018, [Peer-reviewed]
  English, Scientific journal
• シンバスタチン含有α型リン酸トリカルシウムミクロ粒子懸濁液による骨誘導(Bone Induction by α-tricalcium Phosphate Microparticle Emulsion Containing Simvastatin)
  Tateyama Akito; Kato Akihito; Miyaji Hirofumi; Nishida Erika; Iwasaki Yasuhiko; Fujii Syuji; Kawamoto Kohei; Shitomi Kanako; Furihata Tomokazu; Mayumi Kayoko; Sugaya Tsutomu
  Nano Biomedicine, 9, 2, 69, 76, ナノ・バイオメディカル学会, Dec. 2017, [Peer-reviewed]
  English, Scientific journal, 骨移植材の分解性と操作性を改善するため、α型リン酸トリカルシウム(α-TCP)骨ペーストを基にした水-オイル懸濁液を調製した。親油性の抗高脂血症薬であるシンバスタチン(SIM)には骨形成促進作用が認められているため、SIM含有α-TCP骨ペーストを用いてラットの頭蓋骨欠損に対する骨形成効果を評価した。骨ペーストは固化後に多孔性構造とヒドロキシアパタイトの生成を示すので、頭蓋骨欠損部への移植後にX線画像解析と組織学的検査を行った。骨ペーストのSIM含有量は0、0.01、0.1および1mgとし、無SIMの対照骨ペーストと比較した。その結果、骨ペースト移植後に新たな骨形成が認められ、特に0.1mg SIM群では対照群に比べて骨体積が有意に促進され、術後4週では骨欠損部に骨ペースト残渣は見られなかった。以上より、SIM含有α-TCP骨ペーストは分解性であり、骨組織工学にとって有用であることが示唆された。
• Antibacterial and cytotoxic effects of photoexcited Au clusters via blue high-power or white low-power light emitting diode irradiation
  Miyata Saori; Miyaji Hirofumi; Kawasaki Hideya; Nishida Erika; Shitomi Kanako; Akasaka Tsukasa; Tanaka Saori; Iizuka Tadashi; Sugaya Tsutomu
  Biology, Engineering and Medicine, 2, 4, 1, 4, Open Access Text, Oct. 2017, [Peer-reviewed]
  English, Scientific journal, The development of photosensitizers and light sources has enabled the use of antimicrobial photodynamic therapy (aPDT) in various dental therapies. In the presentstudy, we compared the antibacterial and cytotoxic effects of Au clusters photoexcited by blue and white LED irradiation. We fabricated novel photosensitizers,captopril-protected gold (Capt-Au) clusters and lysozyme-stabilized gold (Lyz-Au) clusters, for aPDT. Au clusters were then photoexcited by two kinds of lightsources, blue high-power and white low-power light-emitting diodes (LEDs). Since white LED contains a wide spectrum of light (400–750 nm), white LED wouldbe relevant for aPDT even if using a low-power source.The turbidity and viability of Streptococcus mutans were assessed following application of Capt-Au clusters (500 μg/mL) or Lyz-Au clusters (1,000 μg/mL) photoexcitedby a blue high-power LED (1,000 mW/cm2) or white low-power LED (80 mW/cm2). In addition, the cytotoxicity of Au clusters and LED irradiation was evaluatedin NIH3T3 and MC3T3-E1 cells.Au clusters photoexcited by the white low-power LED equally decreased the turbidity and viability of S. mutans compared with blue high-power LED. However,Au clusters photoexcited by white LED irradiation caused decreased cytotoxicity in mammalian cells compared with those photoexcited by blue LED irradiation. Inconclusion, white LEDs possess biosafe properties for aPDT using Au clusters.
• Dose effects of beta-tricalcium phosphate nanoparticles on biocompatibility and bone conductive ability of three-dimensional collagen scaffolds.
  Shusuke Murakami; Hirofumi Miyaji; Erika Nishida; Kohei Kawamoto; Saori Miyata; Hiroko Takita; Tsukasa Akasaka; Bunshi Fugetsu; Toshihiko Iwanaga; Hiromi Hongo; Norio Amizuka; Tsutomu Sugaya; Masamitsu Kawanami
  Dental materials journal, 36, 5, 573, 583, 26 Sep. 2017, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Three-dimensional collagen scaffolds coated with beta-tricalcium phosphate (β-TCP) nanoparticles reportedly exhibit good bioactivity and biodegradability. Dose effects of β-TCP nanoparticles on biocompatibility and bone forming ability were then examined. Collagen scaffold was applied with 1, 5, 10, and 25 wt% β-TCP nanoparticle dispersion and designated TCP1, TCP5, TCP10, and TCP25, respectively. Compressive strength, calcium ion release and enzyme resistance of scaffolds with β-TCP nanoparticles applied increased with β-TCP dose. TCP5 showed excellent cell-ingrowth behavior in rat subcutaneous tissue. When TCP10 was applied, osteoblastic cell proliferation and rat cranial bone augmentation were greater than for any other scaffold. The bone area of TCP10 was 7.7-fold greater than that of non-treated scaffold. In contrast, TCP25 consistently exhibited adverse biological effects. These results suggest that the application dose of β-TCP nanoparticles affects the scaffold bioproperties; consequently, the bone conductive ability of TCP10 was remarkable.
• Periodontal tissue repair after sealing of the gap in vertical root fracture.
  Tsutomu Sugaya; Mahito Tomita; Youji Motoki; Khurshiduz Zaman; Hirofumi Miyaji; Masamitsu Kawanami
  Odontology, 105, 2, 202, 207, Apr. 2017, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, The aim of this study was to determine whether sealing of fracture gap using adhesive resin through the root canal can prevent inflammation of periodontal tissue, and resealing the incompletely sealed fracture gap from outside can resolve such inflammation in experimentally created vertical root fractures. Vertical root fractures were created in incisor of beagles. In the experimental group, the fracture gap was sealed through the root canal with adhesive resin. After 5 weeks, sites with the clinical attachment level ≥4 mm were further divided randomly into the poor-replanting group and the poor-untreated group. In the poor-replanting group, the tooth was extracted and replanted after resealing the fracture gap with adhesive resin from the outer surface. Sites with clinical attachment level ≤3 mm after 5 weeks were considered as the satisfactory group. The poor-untreated group and the satisfactory group were subjected to no further treatment. The clinical attachment level was evaluated at baseline and after 2, 5, and 9 weeks. After 9 weeks, histological measurements were made to determine the length of the epithelial downgrowth and the area of alveolar bone resorption. The clinical attachment level and the area of bone resorption were significantly smaller in the poor-replanting group and the satisfactory group than in the poor-untreated group (p < 0.05). The results indicate the possibility that periodontal inflammation along the fracture line can be prevented and improved if the fracture gap is sealed.
• Site-level progression of periodontal disease during a follow-up period.
  Yoshiaki Nomura; Toshiya Morozumi; Taneaki Nakagawa; Tsutomu Sugaya; Masamitsu Kawanami; Fumihiko Suzuki; Keiso Takahashi; Yuzo Abe; Soh Sato; Asako Makino-Oi; Atsushi Saito; Satomi Takano; Masato Minabe; Yohei Nakayama; Yorimasa Ogata; Hiroaki Kobayashi; Yuichi Izumi; Naoyuki Sugano; Koichi Ito; Satoshi Sekino; Yukihiro Numabe; Chie Fukaya; Nobuo Yoshinari; Mitsuo Fukuda; Toshihide Noguchi; Tomoo Kono; Makoto Umeda; Osamu Fujise; Fusanori Nishimura; Atsutoshi Yoshimura; Yoshitaka Hara; Toshiaki Nakamura; Kazuyuki Noguchi; Erika Kakuta; Nobuhiro Hanada; Shogo Takashiba; Yasuharu Amitani; Hiromasa Yoshie
  PloS one, 12, 12, e0188670, 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Periodontal disease is assessed and its progression is determined via observations on a site-by-site basis. Periodontal data are complex and structured in multiple levels; thus, applying a summary statistical approach (i.e., the mean) for site-level evaluations results in loss of information. Previous studies have shown the availability of mixed effects modeling. However, clinically beneficial information on the progression of periodontal disease during the follow-up period is not available. We conducted a multicenter prospective cohort study. Using mixed effects modeling, we analyzed 18,834 sites distributed on 3,139 teeth in 124 patients, and data were collected 5 times over a 24-month follow-up period. The change in the clinical attachment level (CAL) was used as the outcome variable. The CAL at baseline was an important determinant of the CAL changes, which varied widely according to the tooth surface. The salivary levels of periodontal pathogens, such as Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, were affected by CAL progression. "Linear"- and "burst"-type patterns of CAL progression occurred simultaneously within the same patient. More than half of the teeth that presented burst-type progression sites also presented linear-type progression sites, and most of the progressions were of the linear type. Maxillary premolars and anterior teeth tended to show burst-type progression. The parameters identified in this study may guide practitioners in determining the type and extent of treatment needed at the site and patient levels. In addition, these results show that prior hypotheses concerning "burst" and "linear" theories are not valid.
• Antimicrobial photodynamic activity and cytocompatibility of Au-25(Capt)(18) clusters photoexcited by blue LED light irradiation
  Saori Miyata; Hirofumi Miyaji; Hideya Kawasaki; Masaki Yamamoto; Erika Nishida; Hiroko Takita; Tsukasa Akasaka; Natsumi Ushijima; Toshihiko Iwanaga; Tsutomu Sugaya
  INTERNATIONAL JOURNAL OF NANOMEDICINE, 12, 2703, 2716, 2017, [Peer-reviewed]
  English, Scientific journal
• Antimicrobial photodynamic activity and cytocompatibility of Au25(Capt)18 clusters photoexcited by blue LED light irradiation.
  Saori Miyata; Hirofumi Miyaji; Hideya Kawasaki; Masaki Yamamoto; Erika Nishida; Hiroko Takita; Tsukasa Akasaka; Natsumi Ushijima; Toshihiko Iwanaga; Tsutomu Sugaya
  International journal of nanomedicine, 12, 2703, 2716, 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Antimicrobial photodynamic therapy (aPDT) has beneficial effects in dental treatment. We applied captopril-protected gold (Au25(Capt)18) clusters as a novel photosensitizer for aPDT. Photoexcited Au clusters under light irradiation generated singlet oxygen (1O2). Accordingly, the antimicrobial and cytotoxic effects of Au25(Capt)18 clusters under dental blue light-emitting diode (LED) irradiation were evaluated. 1O2 generation of Au25(Capt)18 clusters under blue LED irradiation (420-460 nm) was detected by a methotrexate (MTX) probe. The antimicrobial effects of photoexcited Au clusters (0, 5, 50, and 500 μg/mL) on oral bacterial cells, such as Streptococcus mutans, Aggregatibacter actinomycetemcomitans, and Porphyromonas gingivalis, were assessed by morphological observations and bacterial growth experiments. Cytotoxicity testing of Au clusters and blue LED irradiation was then performed against NIH3T3 and MC3T3-E1 cells. In addition, the biological performance of Au clusters (500 μg/mL) was compared to an organic dye photosensitizer, methylene blue (MB; 10 and 100 μg/mL). We confirmed the 1O2 generation ability of Au25(Capt)18 clusters through the fluorescence spectra of oxidized MTX. Successful application of photoexcited Au clusters to aPDT was demonstrated by dose-dependent decreases in the turbidity of oral bacterial cells. Morphological observation revealed that application of Au clusters stimulated destruction of bacterial cell walls and inhibited biofilm formation. Aggregation of Au clusters around bacterial cells was fluorescently observed. However, photoexcited Au clusters did not negatively affect the adhesion, spreading, and proliferation of mammalian cells, particularly at lower doses. In addition, application of Au clusters demonstrated significantly better cytocompatibility compared to MB. We found that a combination of Au25(Capt)18 clusters and blue LED irradiation exhibited good antimicrobial effects through 1O2 generation and biosafe characteristics, which is desirable for aPDT in dentistry.
• Treatment of infected root canals with 4-META/MMA-TBB resin
  Sugaya T; Tanaka S; Miyaji H; Shimoji S; Kudo M; Takefu H; Washizu T
  Hokkaido J Dent, 38, 111, 121, 北海道歯学会, 2017, [Peer-reviewed]
  English, Scientific journal, This review paper describes the various applications of 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate-tri-n-butyl borane (4-META/MMA-TBB) and reviews research studies on the treatment of infected root canals using 4-META/MMA-TBB resin. 4-META/MMA-TBB resin exhibits good biocompatibility, polymerisation, and adhesiveness to dentine. As a result, it has improved the previously low success rates of root-end sealing, root canal filling, and perforation sealing in difficult cases. Root-end sealing of resected surfaces using 4-META/MMA-TBB during apicoectomy and intentional replantation prevents leakage from root canal and root resorption. 4-META/MMA-TBB can offer sealing for root canals with opened root apex in which pressure could not be applied during root canal filling. In this paper we will discuss clinical cases related to the application of this resin and the benefits of 4-META/MMA-TBB resin.
• Assessing the progression of chronic periodontitis using subgingival pathogen levels: a 24-month prospective multicenter cohort study
  E. Kakuta; Y. Nomura; T. Morozumi; T. Nakagawa; T. Nakamura; K. Noguchi; A. Yoshimura; Y. Hara; O. Fujise; F. Nishimura; T. Kono; M. Umeda; M. Fukuda; T. Noguchi; N. Yoshinari; C. Fukaya; S. Sekino; Y. Numabe; N. Sugano; K. Ito; H. Kobayashi; Y. Izumi; H. Takai; Y. Ogata; S. Takano; M. Minabe; A. Makino-Oi; A. Saito; Y. Abe; S. Sato; F. Suzuki; K. Takahashi; T. Sugaya; M. Kawanami; N. Hanada; S. Takashiba; H. Yoshie
  BMC ORAL HEALTH, 17, Jan. 2017, [Peer-reviewed]
  English, Scientific journal
• Assessment of antimicrobial and biocompatible effects of Au25(Capt)18 clusters photoexcited by blue LED
  Miyata S; Miyaji H; Kawasaki H; Yamamoto M; Nishida E; Takita H; Akasaka T; Ushijima N; Iwanaga T; Sugaya T
  Int J Nanomedicine, 12, 2703, 2716, 2017, [Peer-reviewed]
  English, Scientific journal
• α-TCP複合化4-META/MMA-TBBレジン上へのrhBMP-2による骨増生
  猪俣慶久; 菅谷 勉; 川浪雅光
  北海道歯誌, 37, 18, 26, 2017, [Peer-reviewed]
  Japanese, Scientific journal
• Periodontal tissue engineering by nano beta-tricalcium phosphate scaffold and fibroblast growth factor-2 in one-wall infrabony defects of dogs
  K. Ogawa; H. Miyaji; A. Kato; Y. Kosen; T. Momose; T. Yoshida; E. Nishida; S. Miyata; S. Murakami; H. Takita; B. Fugetsu; T. Sugaya; M. Kawanami
  JOURNAL OF PERIODONTAL RESEARCH, 51, 6, 758, 767, Dec. 2016, [Peer-reviewed]
  English, Scientific journal
• Salivary pathogen and serum antibody to assess the progression of chronic periodontitis: a 24-mo prospective multicenter cohort study
  T. Morozumi; T. Nakagawa; Y. Nomura; T. Sugaya; M. Kawanami; F. Suzuki; K. Takahashi; Y. Abe; S. Sato; A. Makino-Oi; A. Saito; S. Takano; M. Minabe; Y. Nakayama; Y. Ogata; H. Kobayashi; Y. Izumi; N. Sugano; K. Ito; S. Sekino; Y. Numabe; C. Fukaya; N. Yoshinari; M. Fukuda; T. Noguchi; T. Kono; M. Umeda; O. Fujise; F. Nishimura; A. Yoshimura; Y. Hara; T. Nakamura; K. Noguchi; E. Kakuta; N. Hanada; S. Takashiba; H. Yoshie
  JOURNAL OF PERIODONTAL RESEARCH, 51, 6, 768, 778, Dec. 2016, [Peer-reviewed]
  English, Scientific journal
• Influence of enamel matrix derivative on healing of root surfaces after bonding treatment and intentional replantation of vertically fractured roots.
  Tsutomu Sugaya; Mahito Tomita; Youji Motoki; Hirofumi Miyaji; Masamitsu Kawamami
  Dental traumatology : official publication of International Association for Dental Traumatology, 32, 5, 397, 401, Oct. 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND/AIM: The objectives of this study were to histopathologically evaluate cementum regeneration on root surfaces when an enamel matrix derivative (EMD) was used to bond a vertically fractured root and to evaluate the effectiveness of EMD in inhibiting root resorption. MATERIAL AND METHODS: A total of 40 roots from 24 maxillary premolars in beagles were used. The root was vertically fractured using a chisel and mallet. Super-Bond was then used to bond the fractured root. In the experimental group, the root surface was treated with ethylenediaminetetraacetic acid and an EMD. The control group received no treatment. The root was then replanted in its original location. Histopathological observation and measurement using image-analyzing software were carried out after 8 weeks. RESULTS: In the experimental group, shallow resorption cavities developed on the root planed surfaces with new acellular cementum appearing over them. In the control group, no new cementum was seen on the planed surfaces, and there was connective tissue joining the roots. In some of the samples, resorption and multinucleated giant cells were seen. The experimental group showed a significantly larger volume of cementum formation (P < 0.001), and the volume of root resorption was significantly smaller (P = 0.004). CONCLUSION: When bonding and replanting tooth roots after a vertical fracture, the application of EMD was effective in regenerating cementum on root surfaces from which periodontal ligament had been lost in the area around the fracture line and in reducing the incidence of root resorption.
• 酸化グラフェンスキャフォールド埋植による歯周組織治癒促進効果
  川本 康平; 宮治 裕史; 加藤 昭人; 西田 絵利香; 小川 幸佑; 舘山 彰人; 降旗 友和; 宮田 さほり; 蔀 佳奈子; 菅谷 勉
  日本歯周病学会会誌, 58, 秋季特別, 110, 110, (NPO)日本歯周病学会, Sep. 2016
  Japanese
• 4-META/MMA-TBBレジンシーラーとレジン製ポイントを用いた根管充填による垂直歯根破折抵抗性
  鷲巣 太郎; 菅谷 勉
  接着歯学, 34, 1, 9, 15, (一社)日本接着歯学会, Apr. 2016
  Japanese
• New bone contact on 4-META/MMA-TBB resin containing calcium carbonate or α -TCP
  柳澤 剛; 菅谷 勉; 川浪 雅光
  北海道歯学雑誌, 36, 2, 54, 62, 北海道歯学会, Mar. 2016, [Peer-reviewed]
  Japanese
• Effects of Aging on Bone Formation Induced by BMP-2 using Low-intensity Pulsed Ultrasound
  YAMAJI Kozo; YOKOYAMA Akihito; MATSUZAKI Kumiko; OHARA Naoko; NISHIYAMA Eriko; SUGAYA Tsutomu; YOSHIYAMA Masahiro
  Journal of Oral Tissue Engineering, 14, 1, 65, 69, Japanese Association of Regenerative Dentistry, 2016
  English,

  The purpose of this study was to evaluate the effects of aging on bone formation induced by Bone Morphogenetic Protein-2 (BMP-2) using low-intensity pulsed ultrasound (LIPUS)in 12-week-old (12w group) and 70-week-old (70w group) Wistar rats.

  The experimental sites (palatal subperiosteal sites) were divided into three sites as follows: BMP site, BMP-2 was implanted; LI-BMP site, LIPUS treatment was performed every three days after implantation of BMP-2; and Control site, neither implantation of BMP-2 nor LIPUS treatment was done.

  At 3 weeks after surgery, thickness of new bone (TNB) was significantly higher in both the BMP and LI-BMP sites than in the Control site (P<0.05). TNB of every site in the 12w group was significantly higher than that of the same experimental site in the 70w group (P<0.05). Newly formed bone was almost integrated with original bone at the LI-BMP site.

  These results suggest that LIPUS enhances the maturation of bone formation induced by BMP-2 even in senescent rats, although the volume of bone decreased with aging.

• Influence of local anesthesia on autonomic nervous activity in healthy young adults: Evaluation of heart rate variability
  Shinji Shimoji; Hitomi Odanaka; Hiroe Takefu; Risa Oshima; Tsutomu Sugaya; Toshiaki Fujisawa; Masamitsu Kawanami
  Dentistry, 6, 5, 2016, [Peer-reviewed]
  English
• Collagen Hydrogel Scaffold and Fibroblast Growth Factor-2 Accelerate Periodontal Healing of Class II Furcation Defects in Dog.
  Takehito Momose; Hirofumi Miyaji; Akihito Kato; Kosuke Ogawa; Takashi Yoshida; Erika Nishida; Syusuke Murakami; Yuta Kosen; Tsutomu Sugaya; Masamitsu Kawanami
  The open dentistry journal, 10, 347, 59, 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: Collagen hydrogel scaffold exhibits bio-safe properties and facilitates periodontal wound healing. However, regenerated tissue volume is insufficient. Fibroblast growth factor-2 (FGF2) up-regulates cell behaviors and subsequent wound healing. We evaluated whether periodontal wound healing is promoted by application of collagen hydrogel scaffold in combination with FGF2 in furcation defects in beagle dogs. METHODS: Collagen hydrogel was fabricated from bovine type I collagen with an ascorbate-copper ion cross-linking system. Collagen hydrogel was mingled with FGF2 and injected into sponge-form collagen. Subsequently, FGF2 (50 µg)/collagen hydrogel scaffold and collagen hydrogel scaffold alone were implanted into class II furcation defects in dogs. In addition, no implantation was performed as a control. Histometric parameters were assessed at 10 days and 4 weeks after surgery. RESULT: FGF2 application to scaffold promoted considerable cell and tissue ingrowth containing numerous cells and blood vessel-like structure at day 10. At 4 weeks, reconstruction of alveolar bone was stimulated by implantation of scaffold loaded with FGF2. Furthermore, periodontal attachment, consisting of cementum-like tissue, periodontal ligament-like tissue and Sharpey's fibers, was also repaired, indicating that FGF2-loaded scaffold guided self-assembly and then re-established the function of periodontal organs. Aberrant healing, such as ankylosis and root resorption, was not observed. CONCLUSION: FGF2-loaded collagen hydrogel scaffold possessed excellent biocompatibility and strongly promoted periodontal tissue engineering, including periodontal attachment re-organization.
• Bone-forming Effects of Collagen Scaffold Containing β-tricalcium Phosphate Nanoparticles on Extraction Sockets in Dogs
  Akihito KATO; Masamitsu KAWANAMI; Hirofumi MIYAJI; Kosuke OGAWA; Takehito MOMOSE; Erika NISHIDA; Syusuke MURAKAMI; Takashi YOSHIDA; Saori TANAKA; Tsutomu SUGAYA
  The Japanese Journal of Conservative Dentistry, 59, 4, 351, 358, The Japanese Society of Conservative Dentistry, 2016, [Peer-reviewed]
  Japanese,

   Purpose: Beta-tricalcium phosphate (β-TCP) exhibits biocompatibility and osteoconductivity and has been used clinically as a bone graft material. However, conventional β-TCP degrades slowly, with residual material frequently inducing aberrant healing. We therefore developed a collagen scaffold containing β-TCP nanoparticles by applying nanoscale dispersing technology. When the nano-β-TCP/collagen scaffold was implanted into rat cranial bone, its degradation and bone augmentation were remarkable when compared with collagen sponge. Accordingly, we evaluated the bone-forming effects of nano-β-TCP/collagen scaffold on extraction sockets in dogs.

   Methods: β-TCP powder (average particle size: 2.3 μm) was pulverized into nanoscale particles and dispersed into distilled water along with the surfactant sodium cholate (0.2 wt%). Collagen sponge was immersed in a dispersion (1 wt%) of β-TCP nanoparticles. This was followed by rinsing and freeze-drying to yield the nano-β-TCP/collagen scaffold. The surface of the scaffold was characterized by SEM. Subsequently, the extraction socket of a maxillary first premolar was filled with nano-β-TCP/collagen scaffold in the experimental group, while collagen sponge was applied to the socket in the control group. Radiographic images of the socket were then obtained at baseline, and at 1, 3 and 5 weeks after surgery. Histological observations were performed at 2 and 5 weeks.

   Results: SEM images showed nanosized (approx. 100 nm) β-TCP particles attached to the fibers of collagen sponge. The interconnected spaces within the collagen sponge were not filled with β-TCP particles. In the experimental group, the extraction socket showed increased radiopacity when compared with the control group. Histological observation at 2 weeks revealed that new bone was present in the socket in the experimental group. In addition, ingrowth of cells and blood vessels was detected in the nano-β-TCP/collagen scaffold. In contrast, only slight new bone growth was seen in the control group. At 5 weeks in the experimental group, the scaffold had disappeared and the extraction socket was fully filled with new bone. In the control group, although new bone was detected, connective tissue and residual collagen sponge were also observed in part of the extraction socket. Newly formed bone area in the experimental group (25.1%) was significantly greater when compared with the control group (7.6%). Residual material area in the experimental group (59.1%) was significantly less when compared with the control group (81.2%).

   Conclusion: Nano-β-TCP/collagen scaffold exhibited high biocompatibility and degradability. New bone formation in tooth extraction sockets of dogs was facilitated by implantation of nano-β-TCP/collagen scaffold.

• Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket.
  Erika Nishida; Hirofumi Miyaji; Akihito Kato; Hiroko Takita; Toshihiko Iwanaga; Takehito Momose; Kosuke Ogawa; Shusuke Murakami; Tsutomu Sugaya; Masamitsu Kawanami
  International journal of nanomedicine, 11, 2265, 77, 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Graphene oxide (GO) consisting of a carbon monolayer has been widely investigated for tissue engineering platforms because of its unique properties. For this study, we fabricated a GO-applied scaffold and assessed the cellular and tissue behaviors in the scaffold. A preclinical test was conducted to ascertain whether the GO scaffold promoted bone induction in dog tooth extraction sockets. For this study, GO scaffolds were prepared by coating the surface of a collagen sponge scaffold with 0.1 and 1 µg/mL GO dispersion. Scaffolds were characterized using scanning electron microscopy (SEM), physical testing, cell seeding, and rat subcutaneous implant testing. Then a GO scaffold was implanted into a dog tooth extraction socket. Histological observations were made at 2 weeks postsurgery. SEM observations show that GO attached to the surface of collagen scaffold struts. The GO scaffold exhibited an interconnected structure resembling that of control subjects. GO application improved the physical strength, enzyme resistance, and adsorption of calcium and proteins. Cytocompatibility tests showed that GO application significantly increased osteoblastic MC3T3-E1 cell proliferation. In addition, an assessment of rat subcutaneous tissue response revealed that implantation of 1 µg/mL GO scaffold stimulated cellular ingrowth behavior, suggesting that the GO scaffold exhibited good biocompatibility. The tissue ingrowth area and DNA contents of 1 µg/mL GO scaffold were, respectively, approximately 2.5-fold and 1.4-fold greater than those of the control. Particularly, the infiltration of ED2-positive (M2) macrophages and blood vessels were prominent in the GO scaffold. Dog bone-formation tests showed that 1 µg/mL GO scaffold implantation enhanced bone formation. New bone formation following GO scaffold implantation was enhanced fivefold compared to that in control subjects. These results suggest that GO was biocompatible and had high bone-formation capability for the scaffold. The GO scaffold is expected to be beneficial for bone tissue engineering therapy.
• Sealing the gap of vertical root fracture through the root canal
  Sugaya T; Natatsuka M; Motoki Y; Inoue K; Tanaka S; Miyaji H; Kawanami M; Sakagami R
  Dntistry, 2016, [Peer-reviewed]
  English, Scientific journal
• Effect of Music Sedation during Local Anesthesia for Healthy Young Adults: Evaluation by Autonomic Nervous Function according to Heart Rate Variability
  Hitomi ODANAKA; Shinji SHIMOJI; Hiroe TAKEFU; Risa OSHIMA; Tsutomu SUGAYA; Toshiaki FUJISAWA; Masamitsu KAWANAMI
  The Japanese Journal of Conservative Dentistry, 59, 1, 9, 21, The Japanese Society of Conservative Dentistry, 2016, [Peer-reviewed]
  Japanese,  Purpose: Many patients experience fear and anxiety towards dental treatment. It is important to reduce the fear and anxiety of local anesthesia for safe and effective dental treatment, because systemic complications frequently occur during local anesthesia. Music sedation is thought to be a convenient method for supporting safe and effective dental treatment, but this method has not been fully investigated in the dental field. The purpose of this study was to evaluate the effect of music sedation during local anesthesia for healthy young adults by measuring the variance of autonomic nervous function (ANF) using a novel system we developed.
   Methods: The study subjects were 22 healthy young volunteers (mean age 26.5±1.9 years). A music sedation phase and no-music sedation phase were performed on each subject. The sequence of these phases was randomly allocated to the subjects using a central allocation method. First, subject anxiety towards dental treatment was evaluated using Corah’s Dental Anxiety Scale. In the no-music sedation phase, the monitoring system was used to assess blood pressure (BP), heart rate (HR), and ANF for 5 minutes prior to anesthesia (pre-anesthesia) in both the sitting and supine positions, 2 minutes during administration of local anesthesia, and 5 minutes after anesthesia (post-anesthesia) in the supine position. In the music sedation phase, the same measurements were taken in each stage after the beginning of music sedation. Furthermore, subject anxiety pre- and post-anesthesia was evaluated using a visual analog scale (VAS). ANF was evaluated by analyzing the low-frequency (LF) and high-frequency (HF) spectral components of ECG R-R intervals. The ratio of LF to HF (LF/HF) reflects sympathetic nervous function (SNF). Statistical analyses were conducted using the Friedman test and Wilcoxon signed-rank test with a significance level of 5%.
   Results: BP, HR, and VAS showed little variance throughout the procedure. LF/HF showed a significantly lower value during local anesthesia than in the pre-anesthesia sitting position and a significantly lower value during the pre-anesthesia sitting position and supine position in the music sedation phase compared with the no-music sedation phase.
   Conclusion: In healthy young adults, SNF decreases significantly in the sitting and supine positions before local anesthesia with music sedation compared with no-music sedation.
• 【歯根部への接着】セメント質への接着と接着を応用した根尖切除術後の根尖部封鎖
  菅谷 勉; 田中 佐織
  接着歯学, 33, 2, 90, 97, (一社)日本接着歯学会, Aug. 2015
  Japanese
• Bone augmentation using a highly porous PLGA/beta-TCP scaffold containing fibroblast growth factor-2
  T. Yoshida; H. Miyaji; K. Otani; K. Inoue; K. Nakane; H. Nishimura; A. Ibara; A. Shimada; K. Ogawa; E. Nishida; T. Sugaya; L. Sun; B. Fugetsu; M. Kawanami
  JOURNAL OF PERIODONTAL RESEARCH, 50, 2, 265, 273, Apr. 2015, [Peer-reviewed]
  English, Scientific journal
• Effect of citric acid application on removing three types of calcium hydroxide and adhesiveness to root dentin
  木村 慎吾; 菅谷 勉; 鷲巣 太郎; 川浪 雅光
  北海道歯学雑誌, 35, 2, 112, 122, 北海道歯学会, Mar. 2015, [Peer-reviewed]
  Japanese
• The effect of combination of mechanical and chemical enlargement through the root canal on sealability of vertical root fracture gap using 4-META/MMA-TBB resin
  西尾 啓英; 菅谷 勉; 川浪 雅光
  北海道歯学雑誌, 35, 2, 101, 111, 北海道歯学会, Mar. 2015, [Peer-reviewed]
  Japanese
• Comparison of fracture sites and post lengths in longitudinal root fractures.
  Tsutomu Sugaya; Megumi Nakatsuka; Kana Inoue; Saori Tanaka; Hirofumi Miyaji; Ryuji Sakagami; Masamitsu Kawamami
  Journal of endodontics, 41, 2, 159, 63, Feb. 2015, [Peer-reviewed], [International Magazine]
  English, Scientific journal, INTRODUCTION: Comparing the epidemiology of fractures originating in the cervical and apical regions may help to understand the causes and risk of a vertical root fracture. We aimed to determine the frequency of vertical root fractures in different fracture sites and how the fracture site relates to fracture direction and post length. METHODS: Teeth diagnosed with a vertical root fracture were retrospectively surveyed for age and sex of the patient, type of tooth, a fracture region in the longitudinal axial direction, site of the fracture, and presence of a post. The fracture region in the longitudinal axial direction was classified as an incomplete fracture, complete fracture, and uncertain. Incomplete fractures were further classified into a fracture originating in the cervical region, a fracture originating in the midregion, and a fracture originating in the apical region. Posts were evaluated by loss of post and length of post. RESULTS: Fractures originating in the cervical and apical region occurred around the same frequency, whereas fractures originating in the midregion were extremely scarce. Of the fractures originating in the cervical region, 36.2% were in a mesial and/or distal site and 57.4% in a buccal and/or lingual site. Of the fractures originating in the apical region, 90.8% were in the buccal and/or lingual site. The number of cases of fractures originating in the apical region decreased with increased post length. CONCLUSIONS: Sites of fracture and post length differed greatly between fractures originating in the cervical region and the apical region, suggesting that risk factors for fractures originating in the cervical and apical regions are different.
• Combination of Root Surface Modification with BMP-2 and Collagen Hydrogel Scaffold Implantation for Periodontal Healing in Beagle Dogs.
  Akihito Kato; Hirofumi Miyaji; Ryosuke Ishizuka; Keisuke Tokunaga; Kana Inoue; Yuta Kosen; Hiroyuki Yokoyama; Tsutomu Sugaya; Saori Tanaka; Ryuji Sakagami; Masamitsu Kawanami
  The open dentistry journal, 9, 9, 52, 9, 2015, [Peer-reviewed], [International Magazine]
  English, Scientific journal, UNLABELLED: Objective : Biomodification of the root surface plays a major role in periodontal wound healing. Root surface modification with bone morphogenetic protein (BMP) stimulates bone and cementum-like tissue formation; however, severe ankylosis is simultaneously observed. Bio-safe collagen hydrogel scaffolds may therefore be useful for supplying periodontal ligament cells and preventing ankylosis. We examined the effects of BMP modification in conjunction with collagen hydrogel scaffold implantation on periodontal wound healing in dogs. MATERIAL AND METHODS: The collagen hydrogel scaffold was composed of type I collagen sponge and collagen hydrogel. One-wall infrabony defects (5 mm in depth, 3 mm in width) were surgically created in six beagle dogs. In the BMP/Col group, BMP-2 was applied to the root surface (loading dose; 1 µg/µl), and the defects were filled with collagen hydrogel scaffold. In the BMP or Col group, BMP-2 coating or scaffold implantation was performed. Histometric parameters were evaluated at 4 weeks after surgery. RESULTS: Single use of BMP stimulated formation of alveolar bone and ankylosis. In contrast, the BMP/Col group frequently enhanced reconstruction of periodontal attachment including cementum-like tissue, periodontal ligament and alveolar bone. The amount of new periodontal ligament in the BMP/Col group was significantly greater when compared to all other groups. In addition, ankylosis was rarely observed in the BMP/Col group. CONCLUSION: The combination method using root surface modification with BMP and collagen hydrogel scaffold implantation facilitated the reestablishment of periodontal attachment. BMP-related ankylosis was suppressed by implantation of collagen hydrogel.
• Adhesive sealing treatment on dental root with 4-META/TBB MMA resin
  川浪 雅光; 菅谷 勉
  北海道歯学雑誌, 35, 1, 2, 7, 北海道歯学会, Sep. 2014, [Peer-reviewed]
  Japanese
• Graphene oxide coating facilitates the bioactivity of scaffold material for tissue engineering
  Erika Nishida; Hirofumi Miyaji; Hiroko Takita; Izumi Kanayama; Maiko Tsuji; Tsukasa Akasaka; Tsutomu Sugaya; Ryuji Sakagami; Masamitsu Kawanami
  JAPANESE JOURNAL OF APPLIED PHYSICS, 53, 6, 1, 7, Jun. 2014, [Peer-reviewed]
  English, Scientific journal
• Application of microscope in diagnosis and treatment of vertical root fracture
  SUGAYA Tsutomu
  The journal of the Japanese Society for Dental Materials and Devices, 33, 3, 178, 181, The Japanese Society for Dental Materials and Devices (JSDMD), May 2014, [Peer-reviewed]
  Japanese
• 接着技法を応用した破折歯の治療術式の検討
  吉山 昌宏; 小田 豊; 山本 一世; 今里 聡; 中村 洋; 川浪 雅光; 菅谷 勉; 林 美加子; 西谷 佳浩
  日本歯科医学会誌, 33, 69, 73, 日本歯科医学会, Mar. 2014
  Japanese
• レジン系シーラーの種類と根充方法が根尖封鎖性に及ぼす影響
  中澤 篤史; 菅谷 勉; 川浪 雅光
  北海道歯学雑誌, 34, 2, 87, 96, 北海道歯学会, Mar. 2014, [Peer-reviewed]
  Japanese
• 4-META/MMA-TBBレジンの硬化条件が レジン表面への骨形成に及ぼす影響
  洲崎 真希; 菅谷 勉; 川浪 雅光
  北海道歯学雑誌, 34, 2, 65, 76, 北海道歯学会, Mar. 2014, [Peer-reviewed]
  Japanese
• Influence of Local Anesthesia and Ultrasonic Scaling on Autonomic Nervous Activity in Healthy Young Adults
  Hitomi ODANAKA; Shinji SHIMOJI; Hiroe TAKEFU; Risa OSHIMA; Kazuki MIYATA; Tsutomu SUGAYA; Toshiaki FUJISAWA; Masamitsu KAWANAMI
  The Japanese Journal of Conservative Dentistry, 57, 6, 519, 529, The Japanese Society of Conservative Dentistry, 2014, [Peer-reviewed]
  Japanese,  Purpose: In order to provide safe dental treatment, its systemic influence should be well understood. Accordingly, we developed a novel system for evaluating autonomic nervous activity during dental treatment. This study examined the variability of autonomic nervous activity during local anesthesia and ultrasonic scaling in healthy young volunteers.
   Methods: Blood pressure, heart rate and autonomic nervous activity were measured using the novel system in 10 subjects aged in their 20 s as follows: in both the sitting (2 min) and supine positions (2 min) before treatment; under local anesthesia (2 min); during ultrasonic scaling (5 min); and in the sitting position after treatment (2 min). R-R intervals obtained from electrocardiogram were divided into high-frequency (HF) and low-frequency (LF) components by power spectrum analysis in order to evaluate autonomic nervous activity. HF components reflect parasympathetic nervous activity while LF/HF reflects sympathetic nervous activity. Data were analyzed using the Steel-Dwass test.
   Results: Blood pressure and heart rate values did not differ significantly between treatments. Parasympathetic nervous activity values tended to increase under local anesthesia and during ultrasonic scaling. Conversely, sympathetic nervous activity values decreased significantly under local anesthesia and ultrasonic scaling compared with that in the sitting position before treatment. Our results suggest that emotional stress may influence autonomic nervous activity more than noxious stimuli during local anesthesia and ultrasonic scaling in healthy young adults.
   Conclusion: Sympathetic nervous activity decreases significantly during local anesthesia and ultrasonic scaling compared with that in the sitting position before treatment in healthy young adults.
• Comparative study of bioactivity of collagen scaffolds coated with graphene oxide and reduced graphene oxide.
  Izumi Kanayama; Hirofumi Miyaji; Hiroko Takita; Erika Nishida; Maiko Tsuji; Bunshi Fugetsu; Ling Sun; Kana Inoue; Asako Ibara; Tsukasa Akasaka; Tsutomu Sugaya; Masamitsu Kawanami
  International journal of nanomedicine, 9, 3363, 73, 2014, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Clinical survey of vertically fractured teeth and theoretical background for bonding treatment
  Sugaya Tsutomu
  Annals of Japan Prosthodontic Society, 6, 1, 14, 19, Japan Prosthodontic Society, 2014, [Peer-reviewed]
  Japanese, 垂直歯根破折274歯の破折状態を後ろ向きに調査した結果，歯頸部からの破折と根尖部からの破折は同程度の頻度で生じており，また，歯頸部からの破折は近遠心方向と頬舌方向のいずれにもみられたが，根尖部からの破折は86.7％が頬舌方向であった．歯根が垂直に破折した場合の歯周組織の炎症は，根管や破折間隙に増殖した細菌が主な原因で，破折間隙の清掃と封鎖を行うことで炎症が消失することが動物実験で確認された．さらに，実際に接着治療を行った231歯の5年後の生存率は，術前に歯周組織破壊が進行している場合は64.6％であったが，歯周組織破壊が進行していなければ82.6％と，十分に保存可能であった．
• Development of a novel system monitoring autonomic response for analyzing influence of dental treatment
  下地 伸司; 小田中 瞳; 宮田 一生; 菅谷 勉; 藤澤 俊明; 川浪 雅光
  北海道歯学雑誌, 34, 1, 23, 27, 北海道歯学会, 15 Sep. 2013, [Peer-reviewed]
  Japanese
• Effect of citric acid application on removal of calcium hydroxide and root canal sealing
  WASHIZU Taro; SUGAYA Tsutomu; KAWANAMI Masamitsu
  北海道歯学雑誌, 33, 2, 82, 93, 北海道歯学会, 15 Mar. 2013, [Peer-reviewed]
  Japanese
• Sealing ability of root canal fillings using Super-Bond SEALER after chemical enlargement
  HONMA Keishi; SUGAYA Tsutomu; KAWANAMI Masamitsu
  北海道歯学雑誌, 33, 2, 72, 81, 北海道歯学会, 15 Mar. 2013, [Peer-reviewed]
  Japanese
• Ultrasonic grinding of vertical fractured gap surfaces using an electric apex locator and the effect on improving sealing ability of vertical root fracture
  KAWABATA Shinya; SUGAYA Tsutomu; KAWANAMI Masamitsu
  北海道歯学雑誌, 33, 2, 53, 61, 北海道歯学会, 15 Mar. 2013, [Peer-reviewed]
  Japanese
• Influence of Initial Periodontal Therapy on Autonomic Nervous Activity in Healthy Young Adults : Evaluation by Heart Rate Variability
  SHIMOJI Shinji; ODANAKA Hitomi; MIYATA Kazuki; SUGAYA Tsutomu; KAWANAMI Masamitsu
  The Japanese Journal of Conservative Dentistry, 56, 5, 431, 441, The Japanese Society of Conservative Dentistry, 2013, [Peer-reviewed]
  Japanese, Purpose: To provide safe periodontal therapy, it is important to understand the systemic influence of such therapy. Therefore, we developed a novel system for evaluating autonomic nervous activity during dental treatment. This study examined the effectiveness of the system for monitoring vital signs, and in so doing, for assessing autonomic nervous activity and evaluating the characteristics of variability in autonomic nervous activity during initial periodontal therapy among healthy volunteers in their twenties. Methods: Blood pressure, heart rate, arterial oxygen saturation and autonomic nervous activity were measured in 10 patients during oral examination, periodontal pocket examination, ultrasonic scaling and impression-taking using the novel system. Autonomic nervous activity was evaluated as follows :R-R intervals of heart rate were divided into high-frequency (HF) and low-frequency (LF) components by power spectrum analysis. HF reflects parasympathetic nervous activity and LF/HF reflects sympathetic nervous activity. Questionnaires concerning the system were completed after treatment. Results: Compared with the resting state, blood pressure, heart rate and arterial oxygen saturation values did not differ significantly among treatments. Conversely, sympathetic nervous activity values tended to increase when sitting, immediately before dental treatment and in the first half of dental treatment. This result suggests that the emotional stress may have a larger influence on autonomic nervous activity than the noxious stimulus during initial periodontal therapy in healthy young adults. Conclusion: This novel system can be used to easily and non-invasively evaluate changes in autonomic nervous activity without stress during initial periodontal therapy. Sympathetic nervous activity values tended to increase when sitting just before treatment and in the first half of the initial periodontal therapy in healthy volunteers in their twenties.
• Influence of Duration of Blood Contamination on Adhesion of 4-META/MMA-TBB Resin to Root Dentin
  TAKEFU Hiroe; SHIMOJI Shinji; SUGAYA Tsutomu; TANAKA Saori; KAWANAMI Masamitsu
  The Japanese Journal of Conservative Dentistry, 56, 5, 407, 414, The Japanese Society of Conservative Dentistry, 2013, [Peer-reviewed]
  Japanese, Purpose: We have previously reported the influence of blood contamination on adhesion of 4-META/MMA-TBB resin to dentin. Our results showed that blood contamination after surface treatment with an aqueous solution of 10% citric acid and 3% ferric chloride (10-3 solution) significantly decreased the adhesion, even if the blood was washed away. If contaminated, it was recommended that the surface be retreated with 10-3 solution and rinsed with water. Therefore, we concluded that blood coagulation inhibits adhesion. In the present study, we evaluated the influence of duration of blood contamination after surface treatment on the leakage and bond strength of 4-META/MMA-TBB resin to dentin. Methods: Bovine root dentin surfaces were contaminated with blood for 2, 5 and 15 seconds after dentin surface treatment with 10-3 solution. The contaminated surface was then rinsed with water (15 seconds), air-dried, or retreated with 10-3 solution (5 seconds). The dye leakage test, microtensile bond strength (MTBS) test, and SEM observation were employed to evaluate the leakage and bond strength of 4-META/MMA-TBB resin to dentin. Results: Results showed that leakage increased and MTBS decreased with an increase in the duration of blood contamination after surface treatment. This is due to the difficulty of removing blood contaminants by simple rinsing as coagulation progresses. However, the leakage value and MTBS were similar to those of the uncontaminated group on re-treatment with 10-3 solution. This may be due to the decalcifying ability and anticoagulant action of citric acid in 10-3 solution, which might effectively remove the blood components. Conclusion: It is recommended that the contaminated surface be thoroughly rinsed with water and re-treated with 10-3 solution, regardless of the duration of blood contamination in the process of adhesion of 4-META/MMA-TBB resin to dentin.
• Effects of Low-intensity Pulsed Ultrasound on Bone Formation Induced by Recombinant Human BMP-2 in Adult Rats
  YAMAJI Kozo; SUGAYA Tsutomu; KAWANAMI Masamitsu; YOSHIYAMA Masahiro; SHIODE Shintaro; ODAJIMA Tomoomi; NISHITANI Yoshihiro; IZAWA Shunji; TANAKA Kumiko; OHARA Naoko; NISHIMURA Maiko; YOKOYAMA Akihito
  The Japanese Journal of Conservative Dentistry, 56, 4, 318, 324, The Japanese Society of Conservative Dentistry, 2013, [Peer-reviewed]
  Japanese, Purpose: The purpose of this study was to evaluate the effects of low-intensity pulsed ultrasound (LIPUS) on bone formation induced by polylactate-polyglycolate-copolymer/gelatin sponge complexes (PGS) containing recombinant human bone morphogenetic protein-2 (rhBMP-2) at palatal subperiosteal sites (n=24) in 15-week-old male Wistar rats. Methods: RhBMP-2/PGS (1.0 μg/μl) was implanted into the palatal subperiosteal site and treated with LIPUS (1.0 MHz, 240 mW, 5 min) every 3 days (LI-BMP group) or without LIPUS (BMP group). In addition, rhBMP-2/PGS (1.0 μg/μl) was not implanted and rats were treated with LIPUS (LI group) or were not treated with LIPUS (Cont group). Animals were euthanized 3 weeks after surgery. Results: Thickness of new bone in LI-BMP and BMP groups was significantly higher when compared with LI and Cont groups (p<0.05). There were no significant differences in thickness of new bone between LI-BMP and BMP groups (p>0.05). Newly formed bone in the BMP group was continuous with the original bone. On the other hand, newly formed bone was largely integrated with the original bone in the LI-BMP group. Conclusion: These results suggest that LIPUS has the ability to enhance maturation of bone induced by rhBMP-2 in the case of palatal subperiosteal implantation in adult rats.
• Osteoconductivity and Biodegradability of Collagen Scaffold Coated with Nano-beta-TCP and Fibroblast Growth Factor 2
  Asako Ibara; Hirofumi Miyaji; Bunshi Fugetsu; Erika Nishida; Hiroko Takita; Saori Tanaka; Tsutomu Sugaya; Masamitsu Kawanami
  JOURNAL OF NANOMATERIALS, 2013, [Peer-reviewed]
  English, Scientific journal
• Treatment of periodontal destruction associated with cemental tear
  SUGAYA Tsutomu; MOTOKI Youji; KAWANAMI Masamitsu
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 54, 4, 307, 314, JAPANESE SOCIETY OF PERIODONTOLOGY, 28 Dec. 2012, [Peer-reviewed]
  Japanese
• Effect of nano-hydroxyapatite on bone morphogenetic protein-2-induced hard tissue formation and dentin resorption on a dentin surface
  Hiroki Tamagawa; Taichi Tenkumo; Tsutomu Sugaya; Masamitsu Kawanami
  APPLIED SURFACE SCIENCE, 262, 140, 145, Dec. 2012, [Peer-reviewed]
  English, Scientific journal
• Application of collagen hydrogel/sponge scaffold facilitates periodontal wound healing in class II furcation defects in beagle dogs
  Y. Kosen; H. Miyaji; A. Kato; T. Sugaya; M. Kawanami
  JOURNAL OF PERIODONTAL RESEARCH, 47, 5, 626, 634, Oct. 2012, [Peer-reviewed]
  English, Scientific journal
• Influence of difference of root canal filling methods on apical sealing in apical foramen widening
  MIKAMI Daisuke; SUGAYA Tsutomu; KAWAMURA Naoto; KAWANAMI Masamitsu
  Hkkaido J Dent Sci, 32, 2, 124, 134, 北海道歯学会, 15 Mar. 2012, [Peer-reviewed]
  Japanese
• Effect of calcium carbonate on bone formation on 4-META/MMA-TBB resin
  HENMI Masaru; SUGAYA Tsutomu; NAKATSUKA Megumi; KAWANAMI Masamitsu
  Hkkaido J Dent Sci, 32, 2, 114, 123, 北海道歯学会, 15 Mar. 2012, [Peer-reviewed]
  Japanese
• Influence of blood contamination before or after surface treatment on adhesion of 4-META/MMA-TBB resin to root dentin.
  Hiroe Takefu; Shinji Shimoji; Tsutomu Sugaya; Masamitsu Kawanami
  Dental materials journal, 31, 1, 131, 8, 03 Feb. 2012, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Connective tissue reaction and bone-cement contact after implantation of PMMA resin cements
  Morishita T; Sugaya T; Nakatsuka M; Sunosaki M; Kawanami M
  J Oral Tissue Engin, 10, 1, 1, 12, Japanese Association of Regenerative Dentistry, 2012, [Peer-reviewed]
  English, Scientific journal, Super Bond (SB) shows biocompatible and adhesive properties to bone as well as to dentin. In this study, SB induced minimal inflammation when implanted in connective tissue of rat, although Multibond II (MB) and Surgical Simplex^® (SX) caused intense inflammatory response. SB was mostly in direct contact with bone without formation of fibrous tissue when implanted in the intramedullary canal of rat, although formation of fibrous tissue between resin cement and bone was mostly observed with MB and SX. Moreover, SEM observation revealed that SB was mostly in direct contact without a gap. Disorders of bone tissue or bone cells were not observed in the case of SB, MB and SX implanted in the intramedullary canal. Thus, 4-META/MMA-TBB cement can be effectively used as a bone cement.
• Healing of experimental apical periodontitis after apicoectomy using different sealing materials on the resected root end.
  Kaori Otani; Tsutomu Sugaya; Mahito Tomita; Yukiko Hasegawa; Hirofumi Miyaji; Taichi Tenkumo; Saori Tanaka; Youji Motoki; Yasuhiro Takanawa; Masamitsu Kawanami
  Dental materials journal, 30, 4, 485, 92, 2011, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Tissue Reaction of 4-META/MMA-TBB Resin used together with Hemostasis Compound
  TAKANAWA Yasuhiro; KAWAMURA Naoto; SUGAYA Tsutomu; KAWANAMI Masamitsu
  Hkkaido J Dent Sci, 31, 2, 35, 43, 北海道歯学会, 15 Dec. 2010, [Peer-reviewed]
  Japanese
• Root surface conditioning with bone morphogenetic protein-2 facilitates cementum-like tissue deposition in beagle dogs
  H. Miyaji; T. Sugaya; K. Ibe; R. Ishizuka; K. Tokunaga; M. Kawanami
  JOURNAL OF PERIODONTAL RESEARCH, 45, 5, 658, 663, Oct. 2010, [Peer-reviewed]
  English, Scientific journal
• Bone Augmentation by Implantation of FGF2-laoded Collagen Gel-sponge Composite Scaffold
  Kobayashi N; Miyaji H; Sugaya T; Kawanami M
  J Oral Tissue Engin, 8, 2, 91, 101, Japanese Association of Regenerative Dentistry, 2010, [Peer-reviewed]
  English, Scientific journal, Fibroblast growth factor-2 (FGF2) plays a critical role in osteoblastic cell proliferation. Collagen gel-sponge composite is an effective scaffold for tissue engineering. The purpose of this study was to evaluate whether addition of FGF2 to collagen gel-sponge composite promotes bone augmentation in rats.
  The rats were assigned to groups designated F0, F3 and F15, and received implantation of collagen gel-sponge composite containing 0, 3 and 15 µg FGF2, respectively, into a cranial bone defect. Specimens were prepared 1, 2 and 5 weeks after surgery for histologic and histomorphometric analysis.
  Newly formed bone area in groups F3 and F15 was significantly greater than that in group F0 at all stages. These results suggest that FGF2-loaded collagen gel-sponge composite scaffold stimulates bone augmentation and might provide a more effective bone engineering approach.
• Periodontal healing by implantation of collagen hydrogel-sponge composite in one-wall infrabony defects in beagle dogs
  Kato A; Miyaji H; Kosen Y; Yokoyama H; Ishizuka R; Tokunaga K; Inoue K; Henmi M; Tanaka S; Sugaya T; Kawanami M
  J Oral Tissue Engin, 8, 1, 39, 46, Japanese Association of Regenerative Dentistry, 2010, [Peer-reviewed]
  English, Scientific journal, The purpose of this study was to evaluate the effect of implanted collagen hydrogel-sponge composite on periodontal wound healing. One-wall infrabony defects (depth: 5 mm; width: 3 mm) were surgically created in three beagle dogs. The exposed root surface was planed and demineralized with EDTA. In the experimental group, the defects were filled with collagen hydrogel-sponge composite. Conversely, no collagen hydrogel-sponge composite was applied to defects in the control group. Histomorphometric parameters were evaluated four weeks after surgery. In the experimental group, regeneration of alveolar bone and cementum was frequently observed. Periodontal ligament tissue was reestablished between the alveolar bone and cementum. New bone height, new bone area, new cementum and new periodontal ligament in the experimental group were significantly greater than those in the control group (p < 0.05). These findings suggest that implanted collagen hydrogel-sponge composite facilitates periodontal wound healing in one-wall infrabony defects in beagle dogs.
• Ectopic Bone Induction by BMP-Loaded Collagen Scaffold and Bone Marrow Stromal Cell Sheet
  Inoue K; Miyaji H; Sugaya T; Kawanami M
  Journal of Oral Tissue Engineering, 8, 1, 19, 29, Japanese Association of Regenerative Dentistry, 2010, [Peer-reviewed]
  English, Scientific journal, We combined the use of bone morphogenetic protein-2(BMP-2)-loaded collagen scaffold and cultured bone marrow stromal cell (BMSC) sheet in order for developing new bone tissue engineering.
  Rat BMSC sheets were generated in the presence of dexamethasone, β-glycerophosphate and ascorbic acid. Four types of the grafts were implanted in the intermuscular region of rat femurs: control group, collagen sponge; cell group, BMSC sheet in collagen sponge; BMP group, BMP-2-loaded collagen sponge; and BMP-cell group, BMSC sheet in BMP-2-loaded collagen sponge. Four weeks after surgery, histological and histomorphometric analysis were performed.
  The BMP-cell group demonstrated abundant new bone formation compared to all the other groups (p<0.05) and marked resorption of the implanted collagen when compared to the control and cell groups (p<0.05).
  The combination of BMP-2-loaded collagen sponge and BMSC sheet appeared to successfully promote an osteoinduction.
• Miyaji H, Sugaya T, Ibe K, Ishizuka R, Tokunaga K, Kawanami M
  Miyaji H; Sugaya T; Ibe K; Ishizuka R; Tokunaga K; Kawanami M
  J Oral Tissue Engin, 8, 3, 173, 180, 2010, [Peer-reviewed]
  English, Scientific journal
• In the forefront of bonding treatment of vertical root fracture
  SUGATANI B.
  Hokkaido J Dent Sci, 30, 1, 3, 10, 北海道歯学会, 15 Jun. 2009, [Peer-reviewed]
  Japanese
• コラーゲンハイドロゲル-スポンジ複合体を頭蓋骨に移植した場合のスキャフォールドとしての有効性とBMP併用による骨増生
  得永 佳介; 宮治 裕史; 石塚 良介; 加藤 昭人; 井上 加菜; 逸見 優; 光銭 裕太; 玉川 博貴; 菅谷 勉; 川浪 雅光
  日本歯周病学会会誌, 51, 春季特別, 110, 110, (NPO)日本歯周病学会, Apr. 2009
  Japanese
• Bone perforation and placement of collagen sponge facilitate bone augmentation.
  Shinji Shimoji; Hirofumi Miyaji; Tsutomu Sugaya; Hifumi Tsuji; Tetsunari Hongo; Megumi Nakatsuka; Khurshid Uz Zaman; Masamitsu Kawanami
  Journal of periodontology, 80, 3, 505, 11, Mar. 2009, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Efect of Crosslinking Agent in Nanohydroxyapatite-collagen Composite on Ectopic Bone Formation with BMP-2 Application
  Tenkumo T; Sugaya T; Kawanami M; Gelinsky M; Watari F
  Nano Biomed, 1, 1, 26, 33, 2009, [Peer-reviewed]
  English, Scientific journal
• Effect of Nano-hydroxyapatite content on bone formation in nano-hydroxyapatite-collagen composite membrane with BMP-2 application
  Tenkumo T; Sugaya T; Kawanami M; Watari F
  Nano Biomedicine, 1, 2, 159, 166, 2009, [Peer-reviewed]
  English, Scientific journal
• Periodontal Tissue Destruction and Bacterial Adhesion at the Surface of Fractures by Cemental Tear
  MOTOKI Youji; SUGAYA Tsutomu; KAWANAMI Masamitsu
  The Japanese Journal of Conservative Dentistry, 52, 5, 411, 418, The Japanese Society of Conservative Dentistry, 2009, [Peer-reviewed]
  Japanese, Cemental tear causes rapid localized destruction of periodontal tissue, but the mechanism remains unclear. Debridement is necessary to remove the bacteria adhered on the root surface, however, debridement in the absence of bacterial adhesion may inhibit the reattachment of the residual periodontal ligament around the fractured surface. This retrospective clinical study was performed to investigate the feature of bacterial adhesion at the root surface of the fractured site or fractured fragment in the case of cemental tear. Eight teeth from 8 patients were diagnosed with cemental tear. The probing depth (PD), bleeding on probing (BOP), suppuration, and rate of bone resorption were measured using dental radiography both before and after the occurrence of the cemental tear. The degree of bacterial adhesion at the root surface of the fractured site or fractured fragment was observed using a scanning electron microscope (SEM). Six patients had visited the hospital before the occurrence of the cemental tear; for these patients, we compared the clinical findings before and after the cemental tear. The appearance of BOP or suppuration, deepened PD, increased rate of bone resorption, and marked periodontal tissue destruction were observed after the cemental tear. A large amount of calculus and considerable bacterial adhesion were observed in 2 cases, a little calculus in 1 case and neither bacteria nor calculus in the remaining 3 cases, at the fractured fragments. For the 2 of the 8 cases, the root surface of an extracted tooth was examined: in 1 case, a high degree of bacterial adhesion was observed, in the other case, neither bacteria nor calculus at the root surface and periodontal ligament tissue around the root surface of the fractured site was observed. The findings of this study indicate that cemental tear may promote advanced periodontal tissue destruction, but bacteria may not always intrude into and adhere to the gap between the root and fractured fragment. If debridement is performed inadvertently without considering the presence of bacterial adhesion, the healing of periodontal tissue may be inhibited because of residual periodontal ligament damage of the root surface.
• Effect of Scaler Tip Shapes and Work Parameters of Air Solfy on Root Surface Roughness and Calculus Removal
  NAKATSUKA Megumi; SUGAYA Tsutomu; KAWANAMI Masamitsu; KATO Hiroshi
  The Japanese Journal of Conservative Dentistry, 52, 6, 460, 468, The Japanese Society of Conservative Dentistry, 2009, [Peer-reviewed]
  Japanese, Sonic and ultrasonic scalers are frequently used for periodontal debridement. It is well known that the shapes of scaler tips, applied pressure, and amplitude range affect the roughness of the tooth surface after scaling. Nowadays, sonic scalers with tunable output power are also available. The clinical technique employed for calculus removal using these sonic scalers also affects the roughness of the tooth surface and the removal of calculus. The purpose of this study was to examine how the shapes of scaler tips and the manner in which this new sonic scaler is used affect the morphology of root surfaces and the removal of calculus. First, flat and smooth dentin specimens (3×3mm) were prepared from extracted teeth, and scaling was performed for 30 seconds under 16 conditions using Air Solfy (J. Morita) and a hand scaler. The control was left untreated. After scaling, the roughness of the specimens was measured with a profilometer, and the morphology of the specimens was examined by SEM. Then, 3×3mm specimens were prepared from extracted teeth with a similar amount of calculus; scaling was performed with two Air Solfy scalers having different tips under three combinations until the test surface appeared smooth and clean on visual inspection, and the instrumentation time was recorded. The following results were obtained: (1) The degree of surface roughness was significantly higher when the tip of the scaler was spherical. (2) The power setting had no effect on the degree of surface roughness when the applied pressure was low; when the power setting was high, however, the surface roughness increased. (3) The degree of surface roughness after scaling with Air Solfy was lower than that after scaling with a hand scaler under most conditions. (4) SEM micrographs revealed that smooth and rough surfaces were intermingled after scaling with Air Solfy. (5) The time required for removal of calculus varied considerably according to the shapes of the scaler tips and the application conditions (p<0.05). Thus, we conclude that Air Solfy can be used to remove calculus with high efficiency and at minimum risk of root damage when the power setting and the applied pressure are high using the spherical tip for removing large amount of calculus, and when the power setting and the applied pressure are low using the straight tip for root planning.
• Effect of calcium phosphate content in nanohydroxyapatite- collagen composite foil with BMP-2 application on ectopic bone formation
  Tenkumo Taichi; Sugaya Tsutomu; Kawanami Masamitu; Watari Fumio
  Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology, 2009, 0, 114, 114, JAPANESE SOCIETY OF PERIODONTOLOGY, 2009, [Peer-reviewed]
  Japanese
• A case of gingival swelling in hematopoietic stem cell transplant recipients during chemotherapy
  Tanaka Saori; Shimoji Shinji; Sugaya Tsutomu; Kawanami Masamitsu
  Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology, 2009, 0, 96, 96, JAPANESE SOCIETY OF PERIODONTOLOGY, 2009, [Peer-reviewed]
  Japanese
• 歯周組織再生療法の臨床研究の現況
  川浪雅光; 齋藤彰; 加藤熈; 菅谷勉; 小田島朝臣; 宮治裕史; 齋藤恵美子
  歯科臨床研究, 5, 2, 71, 81, クインテッセンス出版(株), Jul. 2008
  Japanese
• BMPを用いた歯周組織再生療法の開発
  宮治裕史; 菅谷勉; 加藤昭人; 齋藤彰; 小田島朝臣; 齋藤恵美子; 川浪雅光
  日本歯科評論, 68, 4, 39, 40, (株)ヒョーロン・パブリッシャーズ, Apr. 2008
  Japanese
• Long-term Stability of Adhesion between 4-META/MMA-TBB Resin and Bone
  NAKATSUKA Megumi; KAWAMURA Naoto; SUGAYA Tsutomu; KAWANAMI Masamitsu
  The Journal of the Japanese Society for Dental Materials and Devices, 27, 6, 455, 465, The Japanese Society for Dental Materials and Devices, 2008, [Peer-reviewed]
  Japanese, In this study, we observed the long-term stabiliy of adhesion and the influence of 4-META/MMA-TBB resin on bone. The cranial bone surfaces of rats were surgically exposed and treated with 10% citric acid and 3% ferric chloride agueous solution, and then washed and dried. 4-META/MMA-TBB resin was applied, and the bone surface was observed under LM, SEM, and TEM after 1 day, and 6, 9, and 12 months. The resin was directly adhered to bone via a hybrid layer, and few inflammatory cells were found. The osteocytes showed atrophy and they were absent in the outer layer of the bone which was surgically exposed, pretreated, and dried. After 12 months, there was no invasion of connective tissue in the interface between resin and bone. Bone to which resin was applied was continuous with the surrounding bone and was replaced slowly with newly-formed bone. It is concluded that the adhesion between 4-META/MMA-TBB resin and bone and biocompatibility showed long-term stability.
• Biological Effects of Collagen Hydrogel-sponge Composite Scaffold and Osteoinduction by Combined Application with BMP
  TOKUNAGA Keisuke; SUGAYA Tsutomu; MIYAJI Hirofumi; KAWANAMI Masamitsu
  The Japanese Journal of Conservative Dentistry, 51, 6, 659, 669, The Japanese Society of Conservative Dentistry, 2008, [Peer-reviewed]
  Japanese, Scientific journal, We prepared a collagen hydrogel-sponge composite which would serve as a bioactive scaffold for bone engineering. The present study examined the effects of the collagen composite scaffold combined with/without bone morphogenetic protein (BMP) on tissue reaction and osteoinduction after implantation in rat cranial bone defects. In order to create the collagen hydrogel-sponge composite, 1.5% collagen hydrogel cross-linked by ascorbate and copper ion was injected into 4% FC-HAC sponge (6×6×3mm) which was prepared by mixing fibrillar collagen with heat-denatured collagen. A bone defect (4×4×0.5mm) was made in a calvalium of 87 Wistar male rats and the rats were assigned to five experimental groups according to the implantation. The defects were filled with the collagen hydrogel-sponge composite loaded with BMP-2 (BGS group, n=18), the collagen hydrogel-sponge composite (GS group, n=19), the FC-HAC sponge containing BMP-2 (BS group, n=17), the FC-HAC sponge (S group, n=17), or no materials (C group, n=16). Animals were euthanized after 5, 10, and 15 days, and the specimens were analyzed histomorphologically. In the BGS and GS groups, fibroblastic and osteoblastic cells proliferated on the inner side of the collagen hydrogel-sponge composite. Few inflammatory cells were seen in the connective tissue around the remnants of collagen materials. At day 15, newly formed bone area and height in the BGS group were significantly greater than those in any other group (p<0.01). A significantly greater bone induction was measured in the GS group than in the C group (p<0.01). Implanted collagen material in the BGS group significantly disappeared compared to the S group (p<0.05). These results suggested that collagen hydrogel-sponge composite encouraged the proliferation of fibroblastic and osteoblastic cells, and rapid replacement for regenerating tissue and space making, and has a better potential as a scaffold for osteoinduction by combined implantation of BMP.
• 1壁性骨欠損における歯根象牙質表面へのBMP-2塗布による歯周組織再生効果
  加藤 昭人; 宮治 裕史; 菅谷 勉; 川浪 雅光
  日本歯周病学会会誌, 49, 4, 296, 304, (NPO)日本歯周病学会, Dec. 2007
  Japanese
• A case report of siblings diagnosed with Papillon-Lefevre syndrome
  KAKUDATE Naoki; SUGAYA Tsutomu; ONO Yoshio; KIMURA Kouichi; FUKUSHIMA Chiyuki; FUJISAWA Masako; NAGAYAMA Masato; MORITA Manabu; NOMURA Kazuo; KAWANAMI Masamitsu
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 49, 3, 239, 249, JAPANESE SOCIETY OF PERIODONTOLOGY, 28 Sep. 2007, [Peer-reviewed]
  Japanese, Papillon-Lefèvre syndrome (PLS) is a rare autosomal recessive disease characterized by palmoplantar hyperkeratosis and severe periodontal disease. We reported two siblings with PLS who were diagnosed using a mutation analysis of the cathepsin C gene. The siblings were a 24 year-old woman and a 21 year-old man. They had been referred to the Department of Dentistry and Oral Surgery from the Department of Dermatology, Aomori Prefectural Central Hospital, for a close examination and treatment of their periodontal disease. Both the patients exhibited keratinized erythema on their palms, plantar arches, cubiti, and patellae. The sister had mild plaque-induced gingivitis. However, the brother exhibited profound gingival inflammation and periodontal disease with advanced alveolar bone loss and severe tooth mobility and was subsequently diagnosed as having generalized periodontitis associated with a genetic disorder. Seventeen teeth were extracted, and periodontal surgery under a general anesthesia was performed for ten teeth. Consequently, the pocket depths of his remaining teeth decreased to less than 3 mm at most sites. Although more than 6 years have passed since the initial dental examination, his periodontal tissue has been well maintained and no further periodontal disease has occurred. The absence of severe periodontal destruction in the sister,who carried the same mutation in the cathepsin C gene, suggests that this mutation was not necessarily associated with severe periodontal disease. Moreover, as the brother achieved a favorable improvement after the initial treatment and periodontal surgery,patients with strong disposing genetic factors may be able to slow the progression of periodontal disease by receiving conventional periodontal treatments.
  Nihon Shishubyo Gakkai Kaishi (J Jpn Periodontol) 49 : 239-249, 2007.
• Bonding Treatment of Vertical Root Fracture
  SUGAYA Tsutomu; TANAKA Saori; MIYAJI Hirofumi; TOMITA Mahito; HASEGAWA Yukiko; KAWAMURA Naoto; TANAKA Yuko; MOTOKI Youji; NAKATSUKA Megumi; KAWANAMI Masamitsu; KATO Hiroshi; SANO Hidehiko; SIDHU Sharanbir K.
  日本歯科医学会誌 = Journal of the Japanese Association for Dental Science : JJDS, 0, 26, 47, 51, 31 Mar. 2007, [Peer-reviewed]
  Japanese, Scientific journal
• 垂直歯根破折の接着治療
  菅谷 勉; 田中 佐織; 宮治 裕史; 富田 真仁; 長谷川 有紀子; 川村 直人; 田中 裕子; 元木 洋史; 中塚 愛; 川浪 雅光; 加藤 熈; 佐野 英彦; Sidhu Sharanbir K.
  日本歯科医学会誌, 26, 47, 51, 日本歯科医学会, Mar. 2007
  Japanese
• The Effect of Collagen Hydrogel Implantation in Buccal Dehiscence Defects in Beagles
  Miyaji H; Sugaya T; Kato K; Kawmura N; Kawanami M
  J Oral Tissue Engin, 5, 2, 87, 95, Japanese Association of Regenerative Dentistry, 2007, [Peer-reviewed]
  English, Scientific journal, Type I collagen hydrogel treated by an ascorbate-copper ion crosslinking system is highly biocompatible and highly degradable in the body. The purpose of this study was to examine the effect of collagen hydrogel on periodontal wound healing in beagles. Sixty-four periodontal dehiscence type defects were created on the buccal roots of four beagles. Subsequently, collagen hydrogel was implanted in each defect in the experimental group and sutured, and, in the control group, the defect was sutured without any application of collagen hydrogel. The percentage of the length of new bone and new cementum in the experimental group was significantly greater than that in the control group at weeks 4 (p<0.01) and 8 (p<0.05). Significantly more junctional epithelium was observed in the control group than in the experimental group at weeks 2 (p<0.01) and 4 (p<0.05). These findings thus indicate that periodontal regeneration was stimulated by collagen hydrogel implantation in beagles.
• 動画を用いたHTMLアトラスによる歯周外科手術シミュレーション教育の効率化
  川村直人; 菅谷 勉; 川浪雅光
  日本歯科保存学雑誌, 50, 1, 62, 67, 2007, [Peer-reviewed]
  Japanese, Scientific journal
• Efficiency of HTML Atlas Using Movies for Simulation Training of Periodontal Surgery
  KAWAMURA Naoto; SUGAYA Tsutomu; KAWANAMI Masamitsu
  The Japanese Journal of Conservative Dentistry, 50, 1, 62, 67, The Japanese Society of Conservative Dentistry, 2007, [Peer-reviewed]
  Japanese, Periodontal surgery requires operators to have specific knowledge and technical skills for surgery. Practice on a simulation model may help improve skills. We have developed a simulation model for periodontal surgery. Operators need suitable advice or instructions from an instructor during simulation training using the model. HTML atlases were made for practice manuals of periodontal surgery. The purpose of the study was to investigate the effect of the HTML atlases using movies during simulation training on achievement and time for practice and instruction. Twenty dentists, 1-3 years after graduation, practiced on the simulation model. Ten operators practiced wedge operation referring to the HTML atlas using the movies (experimental group) and the other ten referred to the HTML atlas using the still pictures (control group). The results were as follows: 1. The degree of achievement was not significant between both groups. 2. The average time for practice of the experimental group was shorter than that of the control group. 3. The average time for instruction by the instructor of the experimental group was shorter than that of the control group. These results suggested that the HTML atlas using movies saves time for instruction of simulation training.
• Hybrid layer seals the cementum/4-META/MMA-TBB resin interface.
  Saori Tanaka; Tsutomu Sugaya; Masamitsu Kawanami; Yoshinobu Nodasaka; Toshiki Yamamoto; Hiroshi Noguchi; Yuko Tanaka; Takatsumi Ikeda; Hidehiko Sano; Sharanbir K Sidhu
  Journal of biomedical materials research. Part B, Applied biomaterials, 80, 1, 140, 5, Jan. 2007, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Effects of Air Solfy Brush Tips on plaque removal from concaved root surfaces
  Nakatsuka Megumi; Sugaya Tsutomu; Kawanami Masamitsu; Kato Hiroshi
  Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology, 2007, 0, 109, 109, JAPANESE SOCIETY OF PERIODONTOLOGY, 2007, [Peer-reviewed]
  Japanese
• Hybrid layer seals the cementum/4-META/MMA-TBB resin interface
  Saori Tanaka; Tsutomu Sugaya; Masamitsu Kawanami; Yoshinobu Nodasaka; Toshiki Yamamoto; Hiroshi Noguchi; Yuko Tanaka; Takatsumi Ikeda; Hidehiko Sano; Sharanbir K. Sidhu
  Journal of Biomedical Materials Research - Part B Applied Biomaterials, 80, 1, 140, 145, Jan. 2007, [Peer-reviewed]
  English, Scientific journal
• Suppression of cementum-like tissue formation on BMP-2-applied dentin surface by bisphosphonate
  HONGO Tetsunari; MIYAJI Hirofumi; SUGAYA Tsutomu; KAWANAMI Masamitsu
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 48, 4, 285, 296, JAPANESE SOCIETY OF PERIODONTOLOGY, 28 Dec. 2006, [Peer-reviewed]
  Japanese, We studied the effects of bisphosphonate administration on cementum-like tissue formation and dentin resorption induced by BMP-2 in rats.
  Incadronate (1μg/kg/day) was administered intraperitoneally to rats three times per week, from two weeks before implanting dentin blocks to the end of the observation period. Saline was administered under the same protocol without incadronate to the control group. We prepared 180 dentin blocks from rat teeth roots and treated them with 24%EDTA. Each block had 0,100 or 400μg/ml rhBMP-2 applied. Blocks were then implanted into palatal connective tissue of rats, and specimens were prepared 2, 4, and 8 weeks after implantation for histological and histomorphometric examination.
  Results showed that dentin resorption in the control group was significantly greater than that in the experimental group (p<0.01). New cementum-like tissue formed in the experimental group was also significantly less than that in the control group (p<0.01). 80% of new cementum-like tissue were formed on resorption site of dentin and 20% on the nonresorption site. TRAP positive cell numbers did not show any significant difference between groups.
  These findings suggested that osteoclast-like cells mediated new cementum-like tissue formation, and dentin resorption was suppressed by bisphosphonate administration and osteoclasts this play an important role in has been new cementum-like tissue formation on dentin surfaces to which BMP-2 applied. Nihon Shishubyo Gakkai Kaishi (J Jpn Soc Periodontol) 48 : 285-296, 2006.
• Periodontal regeneration by BMP-2 solution and collagen hydrogel application to root dentin surface
  ISHIZUKA Ryousuke; MIYAJI Hirofumi; SUGAYA Tsutomu; KAWANAMI Masamitsu
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 48, 4, 255, 266, JAPANESE SOCIETY OF PERIODONTOLOGY, 28 Dec. 2006, [Peer-reviewed]
  Japanese, We evaluates periodontal regeneration in a dehiscence model after the application of BMP-2 solution and collagen hydrogel to the root dentin surface.
  Materials were 128 premolars of 8 beagles. After a partial thickness muco-gingival flap on the buccal site was elevated, periodontal dehiscence defects with a length of 6 mm were surgically produced. Root surfaces were scaled and planed to remove periodontal ligament and cementum.
  Denuded dentin surfaces were subsequently demineralized with 24% EDTA and divided into four groups. In the control group, neither BMP-2 solution nor collagen hydrogel was applied. In the Gel group, collagen hydrogel was applied to root dentin surfaces. In the BMP group, BMP-2 (1000 μg/ml) solution was applied to root dentin surfaces. In the BMP-Gel group, BMP-2 (1000 μg/ml) solution and collagen hydrogel were applied to root dentin surfaces. Histological analysis was conducted 1, 2, 4 and 8 weeks after surgery. Histometrical analysis was conducted 4 and 8 weeks after surgery.
  At 8 weeks, new bone formation in the BMP-Gel group was significantly greater than in any other group. New cementum formation in the BMP-Gel group was significantly greater than in the control group. Ankylosis in the BMP-Gel group was significantly greater than in the control group. Down-growth of the junctional epithelium in the BMP-Gel group was significantly smaller than in the control group.
  These results suggest that the application of BMP-2 solution and collagen hydrogel to root dentin surfaces is conducive to periodontal tissue regeneration. Nihon Shishubyo Gakkai Kaishi (J Jpn Soc Periodontol) 48 : 255-266, 2006.
• 歯根象牙質表面へのBMP-2とコラーゲンハイドロゲルの塗布が歯周組織再生を高める
  石塚 良介; 宮治 裕史; 伊部 敬介; 加藤 昭人; 得永 佳介; 井上 加菜; 田中 陽; 菅谷 勉; 川浪 雅光
  日本歯周病学会会誌, 48, 秋季特別, 148, 148, (NPO)日本歯周病学会, Sep. 2006
  Japanese
• Bisphosphonate投与はBMP-2処理した象牙質表面へのセメント質様硬組織形成と象牙質吸収を抑制する
  本郷 哲也; 宮治 裕史; 川村 直人; 天雲 太一; 大谷 香織; 得永 佳介; 菅谷 勉; 川浪 雅光
  日本歯周病学会会誌, 48, 秋季特別, 149, 149, (NPO)日本歯周病学会, Sep. 2006
  Japanese
• Dentin resorption and cementum-like tissue formation by bone morphogenetic protein application
  H Miyaji; T Sugaya; K Kato; N Kawamura; H Tsuji; M Kawanami
  JOURNAL OF PERIODONTAL RESEARCH, 41, 4, 311, 315, Aug. 2006, [Peer-reviewed]
  English, Scientific journal
• Healing of Apical Periodontitis after Root-end Sealing using 4-META/MMA-TBB Resin following Apicoectomy
  HASEGAWA Yukiko; TOMITA Mahito; SUGAYA Tsutomu; KAWANAMI Masamitsu
  Jpn J Conserv Dent, 49, 2, 282, 287, (NPO)日本歯科保存学会, 30 Apr. 2006, [Peer-reviewed]
  Japanese, 根尖性歯周炎を惹起したサルの歯根に根尖切除術を行い,根尖切除面を4-META/MMA-TBBレジンでroot-end sealingして治癒状態を病理組織学的に評価した.X線透過像面積の観察では,実験群が対照群と比較して有意な骨透過像の改善を認めた.組織学的観察では,対照群の根尖部に炎症病変を著明に認めたのに対し,実験群では根尖部の炎症病変は改善し,4-META/MMA-TBBレジンの表面に炎症性細胞浸潤はほとんどみられず,薄い線維で被包されており,骨が近接している歯根が多く観察した.根尖切除術時に4-META/MMA-TBBレジンでroot-end sealingを行うことにより,根管が汚染されたままでも根管内の起炎性物質を根管外に漏洩させず,良好な治癒が得られると考えられた
• クリニカル 垂直歯根破折の診査・診断・接着治療
  菅谷 勉; 加藤 熈
  日本歯科医師会雑誌, 58, 12, 1200, 1210, 日本歯科医師会, Mar. 2006, [Peer-reviewed]
  Japanese
• エアーソルフィーブラシチップによる下顎大臼歯根分岐部のプラーク除去効果
  中塚 愛; 菅谷 勉; 川浪 雅光; 加藤 熈
  日本歯周病学会会誌, 48, 春季特別, 172, 172, (NPO)日本歯周病学会, Mar. 2006
  Japanese
• Effect of Decortication and Onlay Graft of Collagen Sponges on Bony Augmentation
  SHIMOJI Shinji; SUGAYA Tsutomu; MIYAJI Hirofumi; KAWANAMI Masamitsu
  日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 48, 6, 926, 938, 31 Dec. 2005, [Peer-reviewed]
  Japanese, Scientific journal
• Effect of Transplantation of Bone Marrow Exudate on Bony Augmentation
  TSUJI Hifumi; SUGAYA Tsutomu; MIYAJI Hirofumi; KAWANAMI Masamitsu
  日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 48, 6, 917, 925, 31 Dec. 2005, [Peer-reviewed]
  Japanese, Scientific journal
• Effect of Different Dentin Conditionings on Hard Tissue Formation and Dentin Resorption by rhBMP-2 Application to the Dentin Surface
  TENKUMO Taichi; MIYAJI Hirofumi; SUGAYA Tsutomu; KAWANAMI Masamitsu
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 47, 4, 269, 279, JAPANESE SOCIETY OF PERIODONTOLOGY, 28 Dec. 2005, [Peer-reviewed]
  Japanese, Scientific journal, The aim of the present study was to evaluate the effect of different dentin surface conditionings followed by rhBMP-2 application to the dentin surface on the ALP activity of cultured cells that attached to the dentin surface in vitro, and on hard tissue formation on dentin surface and dentin resorption in vivo.
  In the first experiment, 270 dentin blocks were divided into 3 groups, demineralized with EDTA (pH7.0), tetracycline-HCl (pH2.0), and citric acid (pH1.0), respectiveiy, for 3 minutes, and then soaked in 0, 5 or 10 μg/ml rhBMP-2 solution for 10 minutes. Periodontal ligament cells were seeded on each dentin block, and the ALP activity of the attachment cells was assessed on days 1, 3, and 5. In the second experiment, 144 dentin blocks were divided into 4 groups, exposed to EDTA, tetracycline-HCl, citric acid, and PBS, respectively, for 3 minutes, and then exposed to 0, 100, or 400 μg/ml rhBMP-2 solution for 10 minutes. The dentin blocks were later transplanted into the palatal connective tissue of rats, and specimens were prepared at 2 and 4 weeks after surgery.
  The results of the first experiment showed that on day 3 and 5, the EDTA plus application of 10μg/ml rhBMP-2 group had higher ALP activity than the tetracycline-HCl group or citric acid group at any concentration of BMP-2. In the EDTA group, higher rhBMP-2 concentrations were associated with the greater ALP activity. The results of the second experiment demonstrated that the EDTA plus 100μg/ml rhBMP-2 group exhibited significantly higher cementum-like tissue formation any other group at any stage. At week 4, dentin resorption in the tetracycline-HCl plus 400μg/ml rhBMP-2 group was significantly promoted compared to all other groups. Dentin resorption in the EDTA group was not significantly different from the citric acid group. Higher rhBMP-2 concentrations induced higher dentin resorption in all demineralizations groups.
  The results suggest that different dentin conditionings followed by rhBMP-2 application may have influenced the ALP activity of the attached cells in vitro, hard tissue formation on the dentin surface, and dentin resorption in vivo. EDTA demineralization, which induced formation of cementum-like tissue and suppressed dentin resorption, may be an appropriate method of dentin conditioning before rhBMP-2 application to the dentin surface for cementum-like tissue formation in vivo.
• Influence of Width of Resin in Contact with Periodontal Tissue on Apical Migration of Epithelium after Bonding Treatment of Vertical Root Fracture
  MOTOKI Youji; SUGAYA Tsutomu; KAWANAMI Masamitsu
  日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 48, 5, 733, 742, 31 Oct. 2005, [Peer-reviewed]
  Japanese, Scientific journal
• Periodontal Regeneration by BMP-2 Application to Root Dentin Surface
  IBE Keisuke; MIYAJI Hirofumi; SUGAYA Tsutomu; KAWANAMI Masamitsu
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 46, 4, 278, 287, JAPANESE SOCIETY OF PERIODONTOLOGY, 28 Dec. 2004, [Peer-reviewed]
  Japanese, Scientific journal, The objective of this study was to evaluate periodontal regeneration in canine premolars after application of bone morphogenetic protein (BMP)-2 to root dentin surfaces.
  Eighty periodontal defects using a dehiscence model were surgically produced on the buccal roots of premolars of six beagles. After a partial thickness muco-gingival flap was elevated, soft tissues including the periosteum, the periodontal ligament and the cementum were removed. Subsequently, denuded dentin surfaces were demineralized with 24% EDTA, 0, 100, 400, 1000μg/ml BMP-2 solutions were applied, and then labeled as groups 0, 100, 400, 1000, respectively. In the untreated, control group, root dentin was neither demineralized nor was BMP-2 applied. Histological and histometrical analyses were performed at four and eight weeks after surgery.
  At eight weeks, new cementum formation in the 1000 group was significantly greater than the 0 group. Cementum formation in the control group was significantly suppressed compared with all the other groups. Interestingly, cementum-like tissue which was expressed discontinuously to the residual cementum was observed in the 100, 400 and 1000 groups.
  Ankylosis, which was exclusively observed in the coronal part of newly formed bone, was occasionally displayed in groups 400 and 1000 at four weeks. At eight weeks, ankylosis was rarely demonstrated in any group.
  New bone formation extended to the coronal area from the residual alveolar bone in all groups. At eight weeks, new bone in the 400 and 1000 groups was significantly greater than the control and 0 groups.
  Down-growth of the junctional epithelium in the 400 and 1000 groups was significantly smaller than that in 0 group.
  These results suggest that BMP-2 application to the root dentin surface may be conductive to periodontal tissue regeneration.
• Long-term durability of root-end sealing with 4-MEtA/MMA-TBB resin.
  Yuko Tanaka; Tsutomu Sugaya; Saori Tanaka; Masamitsu Kawanami
  Dental materials journal, 23, 4, 453, 6, Dec. 2004, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal
• Effectiveness of Root-end Sealing Using 4-META/MMA-TBB Resin on Healing of Apical Periodontitis
  HASEGAWA Yukiko; SUGAYA Tsutomu; KAWANAMI Masamitsu
  日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 47, 5, 622, 632, (NPO)日本歯科保存学会, 31 Oct. 2004, [Peer-reviewed]
  Japanese, 根尖切除術や再植時に4-META/MMA-TBBレジンでroot-end sealingする方法は有効性が高いと考えられるが,術後の根尖歯周組織の治癒状態を病理組織学的に評価した報告はいまだになされていない.そこで,根管が汚染されたままの状態で4-META/MMA-TBBレジンを用いてroot-end sealingを行った場合と,アマルガムや強化型ユージノールセメントを用いて逆根管充填を行った場合で,根尖歯周組織の治癒状態を比較評価した.4-META/MMA-TBBレジンによるroot-end sealingは有効性が高いことが示唆された
• Durability of adhesion between 4-META/MMA-TBB resin and cementum
  Y Tanaka; T Sugaya; M Kawanami
  DENTAL MATERIALS JOURNAL, 23, 3, 265, 270, Sep. 2004, [Peer-reviewed]
  English, Scientific journal
• Clinical Evaluation of Furcation Perforation Treatment with 4-META/MMA-TBB Resin
  YAMAMOTO Toshiki; SUGAYA Tsutomu; KAWANAMI Masamitsu
  日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 47, 4, 543, 548, (NPO)日本歯科保存学会, 31 Aug. 2004, [Peer-reviewed]
  Japanese, 大臼歯の髄床底に穿孔が認められた陳旧例に対して,4-META/MMA-TBBレジンを用いて非外科的に封鎖した場合の臨床成績を評価した.髄床底部に穿孔が認められた13例16歯を被験歯とした.髄床底穿孔陳旧例に対してスーパーボンドC&Bで非外科的に封鎖し,臨床症状とX線透過像を術前術後に診査し,治癒状態を評価した.術前は臨床症状を有するものが7例に対し,術後は1例のみ臨床症状があり術前に比べ有意に減少した.X線透過像も,術前に透過像の認められた6例中2例の透過像が消失し,1例は縮小傾向,残りの3例には変化はみられず,透過像の拡大は1例もなかった.よって,髄床底穿孔のスーパーボンドC&Bによる非外科的な封鎖は有効であると考えられた
• Biological activities of Bacteroides forsythus lipoproteins and their possible pathological roles in periodontal disease.
  Akira Hasebe; Atsutoshi Yoshimura; Takeshi Into; Hideo Kataoka; Saori Tanaka; Shinichi Arakawa; Hiroaki Ishikura; Douglas T Golenbock; Tsutomu Sugaya; Nobuo Tsuchida; Masamitsu Kawanami; Yoshitaka Hara; Ken-Ichiro Shibata
  Infection and immunity, 72, 3, 1318, 25, Mar. 2004, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Influence of Curing Conditions on Tissue Reaction to the 4-META/MMA-TBB Resin
  KAWAMURA Naoto; SUGAYA Tsutomu; MIYAJI Hirofumi; KAWANAMI Masamitsu
  日本歯科保存学雑誌, 46, 6, 853, 859, (NPO)日本歯科保存学会, 31 Dec. 2003, [Peer-reviewed]
  Japanese, 標題の影響をラットで検討した.空気中でレジンを硬化させると,表面をストリップスで圧接して硬化させた場合や硬化後に表面を研磨した場合に比べて初期の炎症が強かった.骨面を表面処理しレジンを直接塗布して硬化させた場合には炎症性細胞浸潤が殆どみられず,12週後も骨とレジンがハイブリッド様組織を介して接していた.結合組織にレジンを直接塗布して硬化させた場合には結合組織とレジンの界面にハイブリッド様組織がみられ,経時的に炎症性細胞浸潤は消退したものの,多核巨細胞は12週後も残存していた
• Bonding treatment of vertical root fracture
  菅谷 勉
  北海道歯学雑誌, 24, 2, 254, 256, 北海道歯学会, 15 Dec. 2003, [Peer-reviewed]
  Japanese
• Tissue reaction to the surface of 4-META/MMA-TBB resin
  KAWAMURA Naoto; MOTOKI Youji; TSUJI Hifumi; MIYAJI Hirofumi; SUGAYA Tsutomu; KAWANAMI Masamitsu
  日本歯科保存学雑誌, 46, 秋季特別, 46, 46, (NPO)日本歯科保存学会, 07 Oct. 2003, [Peer-reviewed]
  Japanese
• Influence of the Composition and the Concentration of Collagen as a Scaffold for My eloid stem Cell on Healing of Bone Defects
  KATO Katsuya; SUGAYA Tsutomu; MIYAJI Hirofumi; KAWANAMI Masamitsu
  日本歯科保存学雑誌, 45, 6, 1032, 1043, 31 Dec. 2002, [Peer-reviewed]
  Japanese
• Accessibility of an ultrasonic furcation tip to furcation areas of mandibular first and second molars.
  Tsutomu Sugaya; Masamitsu Kawanami; Hiroshi Kato
  Journal of the International Academy of Periodontology, 4, 4, 132, 7, 4, Oct. 2002, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The purpose of this study was to compare an ultrasonic furcation tip with an ultrasonic conventional tip and a hand scaler on accessibility to furcation areas of mandibular first and second molars. The study was conducted on 360 artificial molars that were replicated from 30 mandibular first molars and 30 second molars with silicone impression material and resin. The furcation areas of each molar were coated with red nail colour for artificial calculus. The root areas from the cement-enamel junction apically were covered with silicone rubber simulating gingival tissue. Then the models were instrumented on the buccal or lingual aspects by an experienced operator with each of the following instruments: an ultrasonic furcation tip, an ultrasonic conventional tip and a hand scaler. After instrumentation the percentage of the furcation area with residual artificial calculus was assessed using a computerised imaging system. Results showed that when the horizontal pocket depth was less than 2mm, all three instruments showed good accessibility. When the horizontal pocket depth was more than 2mm, the ultrasonic conventional tip and the hand scaler showed less removal of artificial calculus than the ultrasonic furcation tip (P < 0.01). Efficiency of the ultrasonic furcation tip was fairly satisfactory for the horizontal pocket up to the ridge of the furcation roof.
• Effects of debridement with an ultrasonic furcation tip in degree II furcation involvement of mandibular molars.
  Tsutomu Sugaya; Masamitsu Kawanami; Hiroshi Kato
  Journal of the International Academy of Periodontology, 4, 4, 138, 42, 4, Oct. 2002, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The purpose of this study was to evaluate the clinical effects of closed debridement with an ultrasonic furcation tip in degree II furcation involvement of mandibular molars. Fifteen patients with degree II furcation involvement in mandibular first and/ or second molars participated in the study. All patients were given oral hygiene instruction. Plaque score, probing depth, probing attachment level and bleeding on probing were recorded at baseline, 1, 4, 8 and 12 weeks. Following a baseline examination, a single episode of supra- and subgingival debridement was provided using an ultrasonic furcation tip in the test group, and an ultrasonic conventional tip and hand scalers under local anesthesia in the control group. At baseline, the mean horizontal probing depth in the test group was 6.35 mm and in the control group 6.06 mm. These values decreased to 4.88 mm and 5.29 mm respectively after 12 weeks. There were horizontal probing attachment gains of 1.29 mm in the test group and 0.59 mm in the control group after 12 weeks (P < 0.01). The bleeding scores were 1.0 in the test group and 0.88 in control group at baseline. These scores were improved to 0.12 in the test group and 0.59 in the control group after 12 weeks (P < 0.01). The results suggest that ultrasonic debridement with a furcation tip has a significantly better potential in treating degree II furcation involvement of mandibular molars.
• Hard tissue formation on dentin surfaces applied with recombinant human bone morphogenetic protein-2 in the connective tissue of the palate.
  Hirofumi Miyaji; Tsutomu Sugaya; Tetsuro Miyamoto; Katsuya Kato; Hiroshi Kato
  Journal of periodontal research, 37, 3, 204, 9, Jun. 2002, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• 歯根端切除時に4-META/MMA-TBBレジンをroot-end sealantとして用いた場合の臨床成績
  菅谷 勉; 野口 裕史; 長谷川 有紀子; 田中 裕子; 川浪 雅光
  日本歯科保存学雑誌, 45, 1, 62, 67, (NPO)日本歯科保存学会, Feb. 2002
  Japanese, 当院患者29名(男3名,女26名,平均53.9歳)を対象に,歯根端切除術に伴いスーパーボンドC&Bをroot-end sealantとして用い,約6ヵ月後に術前と同様の診査を行って治癒状態を評価した.その結果,以下の結論が得られた.1)術後6ヵ月には腫脹,圧痛,瘻孔,打診痛,X線写真による骨吸収のいずれにも有意な改善が認められた.2)術前後のX線写真による骨吸収形態の変化を考慮して分類した治癒形態は,完全治癒が22例(61.1%),不完全治癒8例(22.2%),不確実治癒4例(11.1%),失敗2例(5.6%)であった.3)術前に根管拡大を行った場合と行わなかった場合で,逆根管充填後の治癒の分類を比較した結果,有意差は認められなかった
• Efficiency of an Atlas Usin.htmL on Simulation Training of Periodontal Surgery
  OKUMURA Takehito; SUGAYA Tsutomu; KAWANAMI Masamitsu; KATO Hiroshi
  日本歯科保存学雑誌, 44, 6, 973, 978, (NPO)日本歯科保存学会, 01 Dec. 2001, [Peer-reviewed]
  Japanese
• Periodontal healing after bonding treatment of vertical root fracture
  T Sugaya; M Kawanami; H Noguchi; H Kato; N Masaka
  DENTAL TRAUMATOLOGY, 17, 4, 174, 179, Aug. 2001, [Peer-reviewed]
  English, Scientific journal
• Periodontal healing after replantation of intentionally rotated teeth with healthy and denuded root surfaces
  M Kawanami; T Sugaya; H Gama; N Tsukuda; S Tanaka; H Kato
  DENTAL TRAUMATOLOGY, 17, 3, 127, 133, Jun. 2001, [Peer-reviewed]
  English, Scientific journal
• Improvement in Success Rate and Extension of Indication of Intentional Replantation Method
  KATO Hiroshi; SUGAYA Tsutomu; KAWANAMI Masamitsu; OHMORI Mikio; NOGUCHI Hiroshi; OKUMURA Takehito; NINOMIYA Akira; KIMURA Kiyoshi; YAMAMOTO Toshiki
  日本歯科医学会誌 = Journal of the Japanese Association for Dental Science : JJDS, 20, 0, 69, 79, 31 Mar. 2001, [Peer-reviewed]
  Japanese, Scientific journal
• Cementum-like Tissue Formation on Implanted Dentin Blocks with Cultured Prtiodontal Ligament Cells in Cranial Bone Defects of Rats
  Masamitsu Kawanami; Mikio Ohmori; Takehito Okumura; Tsutomu Sugaya; Hiroshi Kato
  Dentistry in Japan, 37, 113, 116, 2001, [Peer-reviewed]
  English, Scientific journal
• Multivariate analysis of relationships between the length of subgingival plaque reformation and periodontal parameters
  KAWANAMI Masamitsu; FUJII Miya; TANAKA Saori; KATO Hiroshi; SUGAYA Tsutomu
  北海道歯学雑誌, 21, 2, 350, 358, 15 Dec. 2000, [Peer-reviewed]
  English
• 再形成した歯肉縁下プラークの長さと臨床診査結果との多変量相関分析(Multivariate analysis of relationships between the length of subgingival plaque reformation and periodontal parameters)
  川浪 雅光; 藤井 美弥; 田中 佐織; 加藤 熈; 菅谷 勉
  北海道歯学雑誌, 21, 2, 350, 358, 北海道歯学会, Dec. 2000
  English, 歯肉縁下付着プラークの歯軸方向の長さと最も強く関連しているのは,ポケットの深さであり,次に象牙質薄片除去時のプロービング時の出血の有無,そして,薄片挿入前の歯垢指数,薄片挿入前のプロービング時の出血の有無の順であった.又,歯周臨床診査結果と再形成した縁下プラークの長さとの間の重相関係数は0.7938で,これらの臨床診査結果が歯肉縁下付着プラークの歯軸方向の長さに影響する寄与率は0.6301であった.本研究から,歯周臨床診査結果とくにポケットの深さが,デブライドメント後に再形成する歯肉縁下プラークの長さと密接な関連があることが示された
• Histopathological Evaluation of Periodontal Healing after Treatment of Furcation Perforations with 4-META/MMA-TBB Resin
  YAMAMOTO Toshiki; SUGAYA Tsutomu; KATO Hiroshi
  日本歯科保存学雑誌, 43, 6, 1239, 1248, (NPO)日本歯科保存学会, 01 Dec. 2000, [Peer-reviewed]
  Japanese, ネコを用いて髄床底を人工的に穿孔させ,封鎖なし(A群),強化型酸化亜鉛ユージノールセメントで封鎖(B群),4-META/MMA-TBBレジン(ME)を用いて筆積法で封鎖(C群),MEのモノマーとキャタリストを混和した活性化液を塗布した後にMEで封鎖(D群)の4群に分けて処置4週後の病理組織学的所見を検討した.その結果,A群は歯根膜への炎症性細胞浸潤が4群中最も大きく,その表面には壊死組織や上皮組織を認めた.B群は材料に接している歯根膜に炎症反応が強く起こるものを認め,その界面には壊死組織を認めた.C群は炎症性細胞浸潤が4群中で最も少なく,歯根膜との界面にヘマトキシリンに染まる無構造な層を認め,歯根膜にレジンがハイブリッドした層と考えられた.D群ではC群と同様の無構造な層を認めたが,その下に炎症反応を広範囲に認めた.髄床底穿孔の治療に,活性化液の塗布を行わずMEを用いる方法は,有用性が高いことが示唆された
• Histopathological Study of Periodontal Destruction after Vertical Root Fracutres
  KIMURA Kiyoshi; SUGAYA Tsutomu; KATO Hiroshi
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 42, 4, 255, 266, The Japanese Society of Periodontology, 01 Dec. 2000, [Peer-reviewed]
  Japanese, Scientific journal, We studied the process of periodontal destruction after vertical root fracture involving 56 upper and lower premolars of 4 beagls. After separation, the root was vertically fractured with a chisel and mallet. Gingival index (GI), probing depth (PD) and probing attachment loss (PAL) were examined clinically. Animals were sacrificed 3 days or 1, 2, or 4 weeks after fracture and radiographs were taken. Epithelial downgrowth, bone resorption, and root resorption were measured histometrically.
  After 3 days or 1 week, PD and PAL increased slightly. Epithelial downgrowth did not occur and bacteria accumulated slightly in the root canal. Inflammation of periodontal ligament was very minimal. After 2 weeks, PD was 4.6±1.7 mm, PAL 3.0±1.6 mm, and epithelial downgrowth 0.2±0.2 mm. Bacteria accumulated in the root canal and on the fracture surface. The periodontal ligament was inflamed and bone and root had resorbed slightly at the fracture site. After 4 weeks, PD was 5.5±1.4 mm, PAL 4.6±1.3 mm, and epithelial downgrowth 0.5±0.3 mm. Plaque had accumulated on root surface, the periodontal ligament was increasingly inflamed and fiber disappeared. Resorption of bone and root had deteriorated and radiography revealed wedge-shaped bone defect.
  These results showed that attachment loss was minimal in the early fracture stage even if probing depth was considerable, and irritants such as bacteria in the root canal induced inflammation of periodontal tissues. Attachment was later lost along the fracture site and periodontal destruction progressed with plaque accumulating on root surfaces. J. Jpn. Soc. Periodontol., 42: 255-266, 2000.
• Effect of rhBMP-2 Applied Dentin on Alkaline Phosphatase Activity and Mineralized Nodule Formation in Gingival Fibroblasts
  MIYAJI Hirofumi; SUGAYA Tsutomu; KATO Hiroshi
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 42, 4, 247, 254, The Japanese Society of Periodontology, 01 Dec. 2000, [Peer-reviewed]
  English, The purpose of this study was to examine the effects of rhBMP-2 applied dentin on alkaline phosphatase (ALP) activity and mineralized nodule formation in gingival fibroblasts in vitro with a view toward developing periodontal regenerative therapy inducing hard tissue to form on the root surface by gingival connective tissue in vivo.
  Dentin blocks were demineralized with EDTA, divided into 5 groups, then labeled based on the treatment, i. e., groups 0, 1, 5, and 10 with 0, 1, 5, and 10. μg/ml rhBMP-2 applied and group PL treated with only DMEM.
  Human gingival fibroblasts (HGF) were seeded on each dentin block in groups 0, 1, 5, and 10 and human periodontal ligament cells (HPLC) on those of group PL. ALP activity and protein concentration of attached cells were assessed on days 1, 3, and 7.
  Dentin blocks in each group were then placed over the confluent HGC and HPLC monolayers and incubated in a-MEM supplemented with β-glycerophosphate. The area of mineralized nodules around dentin blocks was stained by the Von Kossa method and measured on days 5, 7, and 10.
  On day 1, the attached HGF in groups 0, 1, 5, and 10 showed lower ALP activity than that in group PL. On day 3, groups 1, 5, and 10 had about 6 times higher ALP activity than group 0 (p<0.01), and almost the same level as group PL (p>0.05). On day 7, ALP activity in groups 1 and 5 decreased, whereas that in group 10 showed no significant difference compared to the PL group (p>0.05). The amount of mineralized nodule in groups 1, 5, and 10 was about 2.5 times higher than that in group 0 (p<0.05), and was not significant compared to the PL group on day 7 (p>0.05). RhBMP-2 applied dentin thus enhanced ALP activity and mineralized nodule formation in HGF to the level in HPLC. These results suggest that the application of rhBMP-2 may induce hard tissue formation on the root surface by gingival connective tissue. J. Jpn. Soc. Periodontol., 42: 247-254, 2000.
• A study of attached and oriented human periodontal ligament cells to periodontally diseased cementum and dentin after demineralizing with neutral and low pH etching solution
  KU Zaman; T Sugaya; O Hongo; H Kato
  JOURNAL OF PERIODONTOLOGY, 71, 7, 1094, 1099, Jul. 2000, [Peer-reviewed]
  English, Scientific journal
• Topical Application of 2% Minocycline Hydrochloride Ointment effects on the Acute Phase of Periodontitis
  UMEDA Makoto; HAGIWARA Satsuki; UOSHIMA Mariko; TOMINAGA Yumiko; TAKAMORI Yukiko; YANO Kazuko; KOJIMA Takehisa; NOGUCHI Kazuyuki; KIMURA Shintaro; HONGO Okito; IWANAMI Tomotoshi; SAKAGAMI Ryuuji; SUGAYA Tsutomu; NISHIOKA Toshiaki; NEGISHI Jun; MATSUMOTO Atsushi; ITOU Yutaka; SAITOU Akira; KAWANAMI Masamitu; WADA Ikuo; KATO Hiroshi; ISHIKAWA Isao
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 41, 4, 436, 449, The Japanese Society of Periodontology, 28 Dec. 1999, [Peer-reviewed]
  Japanese, Scientific journal, The acute phase of periodontitis (periodontal abscess) has been thought to be related with rapid periodontal breakdown. The effects of topical application of 2% minocycline hydrochloride ointment into the periodontal pockets in the acute phase of periodontitis were studied. One hundred patients with acute periodontitis including periodontal abscesses participated in this study. Patients were assigned randomly to either the test or control groups and compared by a blind method. Acute periodontitis including the periodontal abscesses were treated by subgingival irrigation plus application of 2% minocycline hydrochloride (test group) or by subgingival irrigation only (control group), and examined clinically at baseline, third and seventh days. Microbiological samples were also collected at the baseline and seventh days. The study was performed based on the standards of Good Clinical Practice (GCP). Though the clinical improvements were observed in both groups after treatment, the test group had better results than the control group. The test group showed better improvement of probing depth and attachment level than the control group. On bacteriological examination, both groups exhibited reduction of colony-forming units (CFU) of subgingival plaque bacteria, and higher reduction was observed in the test group. In a comparison of periodontopathic bacteria detection, the frequencies of detection of Bacteroides forsythus, Porphyrornonas gingivalis and Treponema denticola were reduced only in the test group. These results indicate that the topical application of 2% minocycline hydrochloride ointment was effective for treatment of the acute phase of periodontitis. J. Jpn. Soc. Periodontol., 41 : 436-449, 1999.
• Effect of recombinant human platelet-derived growth factor-BB and bone morphogenetic protein-2 application to demineralized dentin on early periodontal ligament cell response
  KU Zaman; T Sugaya; H Kato
  JOURNAL OF PERIODONTAL RESEARCH, 34, 5, 244, 250, Jul. 1999, [Peer-reviewed]
  English, Scientific journal
• A Study of Kinetic Aspects of Cultured and Implanted Periodontal Ligament Cells and Periodontal Regeneration
  OKUMURA Takehito; SUGAYA Tsutomu; KATO Hiroshi
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 40, 4, 421, 430, The Japanese Society of Periodontology, 28 Dec. 1998, [Peer-reviewed]
  Japanese, The purpose of this study is to promote a new regenerative procedure for periodontal tissue by evaluating the kinetic aspects of cultured and implanted periodontal ligament cells. In the experimental group, periodontal ligament cells were cultured and labeled on the dentin blocks in Minimum Essential Medium (MEM) supplemented with bromodeoxyuridine (BrdU) and implanted on the syngeneic rats calvaria defects. In the control group, dentin blocks without cultured cells were implanted. The samples were prepared for histological examination on 7, 14, 21 and 28 days after implantation. Specimens were made with Historesin plus ®, immunostained with anti BrdU monoclonal antibody, and stained for alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP). In the control group, on the 7th day TRAP-positive cells were detected, on the 14th day TRAP-positive and ALP-positive cells were observed. Ankylosis was observed on the 14th day, which was advanced on 21 and 28 days. In the experimental group, results were similar to those of control group on the 7th day. On 14, 21 and 28 days, cellular cementum-like tissue was observed on the dentin blocks in some of the specimens. BrdU positive fibroblast-like cells, ALP-positive cells and TRAP-positive cells were also detected around the newly formed cellular cementum-like tissue.
  These results suggest that the cultured and implanted periodontal ligament cells are able to persist on 28 days after implantation, and may be concerned with the formation of new cementum-like tissue and inhibit ankylosis.
• Influence of the Nature of Dentin Surfaces on Proliferation and Mineralization Activity of Gingival and Periodontal Ligament Cells
  Muhammad Abul; Hassan Mahmud; Tsutomu Sugaya; Hiroshi Kato
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 40, 4, 411, 420, The Japanese Society of Periodontology, 1998, [Peer-reviewed]
  English, Scientific journal, The purpose of this study was to investigate the proliferation and mineralization activity of the human gingival and periodontal ligament cells in relation to the nature of the dentin surface. Three types of bovine dentin blocks were used: 1) Rp group: dentin block was root planed by hand scaler, 2) CA group: dentin block was treated by citric acid pH 1 for 3 mins and 3) Na group: citric acid treated block was further exposed to 10% NaOCl for 3 mins. Besides this, hydroxyapatite (HA) blocks were taken as 4th group. Human gingival cells (HGC) and periodontal ligament cells (HPLC) were seeded on the blocks and cultured. Alkaline phosphatase (ALP) activity and protein concentration of the attached cells on blocks were measured at the 1, 3, 5, 7, 10 and 14 days interval. Furthermore, HGC and HPLC were cultured in DMEM and the blocks were kept on the cell sheet after becoming confluent, media was changed to α-MEM supplemented with 100 nM dexamethasone and 10 mMβ-glycerophosphate. Mineralized nodules were stained weekly for 3 weeks by von-Kossa method and the area was measured in relation to the margin of blocks. The results of the protein concentration of the HGC was higher than the HPLC in the 4 groups. Among the 4 groups, protein concentration of HPLC increased at a faster rate in CA group and in HGC, protein concentration increased at a slower rate in Na group. HGC expressed very low ALP activity and was significantly lower than the HPLC. HPLC showed the highest activity in relation to CA group, and lowest activity was observed in relation to Na and HA group. Regarding the mineralized nodule; HPLC formed larger area of nodule than HGC. CA group formed the largest area of nodule in HPLC but there was no difference among the 4 groups in the HGC. The results suggest that the nature of the dentin surface could influence the cellular proliferation and mineralization activity of HPLC, and also the proliferation of HGC. As HGC are able to show mineralization activity in this study though it is very low, it can be suggested that HGC have the potentiality to form calcified tissue.
• 培養・移植した歯根膜細胞の動態と歯周組織再生の研究
  奥村 健仁; 菅谷 勉; 加藤 熈
  日本歯周病学会誌, 40, 4, 421, 430, (NPO)日本歯周病学会, 1998
  Japanese, ラットの歯根膜細胞を培養し,ラット象牙質片上に播種した後,更にBrdU添加培地で培養することで細胞を標識し,近親交配ラットの頭蓋骨に作成した骨欠損部へ移植して実験群とした.対照群は移植7日後TRAP陽性細胞が認められ,14日後ALP陽性,TRAP陽性細胞が多くなった.14日後からは象牙質と骨とのアンキローシスが進行し21,28日後は全てアンキローシスしていた.実験群の移植7日後は対照群と類似しており,14,21,28日では幾つかの標本の象牙質片上に骨とは非連続性で内部に細胞が封入されたセメント質様硬組織が新生されていた.この硬組織周囲にはALP陽性,TRAP陽性細胞と共にBrdU陽性の線維芽細胞様細胞が観察された
• An Evaluation of Efficacy of Computed Tomography in the Diagnosis of Intrabony Defects
  IWAHARA Koichi; SUGAYA Tsutomu; KATO Hiroshi; KOHIRAZAWA Hideo
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 39, 4, 513, 519, The Japanese Society of Periodontology, 28 Dec. 1997, [Peer-reviewed]
  Japanese, Scientific journal, The purpose of this study was to evaluate the efficacy of the 3-dimensional computed tomogra-phy image (3D-CT) for diagnosis of the morphol-ogy of intrabony defects in periodontal patients.
  Thirty-eight tooth surfaces of 5 periodontal patients, (2 female and 3 male) were selected. The intrabony defects were confirmed by oral photogra-phy during a flap operation. Ten periodontists excluding the flap operator diagnosed them by the 3 D-CT, (CT method), radiography only, (R method), and radiography plus pocket probing (R+P method). The items of diagnosis were presence or absence of intrabony defects on the buccal and lingual aspect, interproximal aspect, and buccal and lingual bony wall in interproximal intrabony defects. Diagnostic accuracy was evaluated by receiver operating characteristic (ROC) analysis. The results were as follows: 1) The diagnosis of the intrabony defects on the buccal and lingual surface by the CT method was signifficantly more accurate than by the R method, but equal to the R+ P method. 2) The accuracy of diagnosis of the interproximal intrabony defects was almost equal among the three methods. 3) The buccal and lin-gual bony wall in interproximal intrabony defects was diagnosed more accurately by the CT method than the other two methods. From the above points of view, it is considered that 3D-CT is effi-cient in diagnosing the intrabony defects of alveolar bone.
  Thus, 3D-CT may have great ability to diagnose the intrabony defects of alveolar bone.
• Treatment of Vertically Fractured Root by Cohesion Part 2. Histological Evaluation on Cohesion with Adhesin Cement
  NOGUCHI Hiroshi; SUGAYA Tsutomu; KATO Hiroshi
  Jpn J Conserv Dent, 40, 6, 1453, 1460, (NPO)日本歯科保存学会, 01 Dec. 1997, [Peer-reviewed]
  Japanese, 1)破折歯根片を接着せず整復して再植した群は,破折部へのセメント質の添加は認められず,歯根の吸収が著しく,有効な治療法と成り得ない. 2)スーパーボンドC&B群は,炎症性吸収及び置換性吸収が最も少なく,健全歯根面率が他の群に比べて有意に大きく,4週後では抜歯後歯根を破折せず再植した群と有意差は認められなかった. 3)パナビア 21群は,炎症性吸収が最も多く,4週後では健全歯根面率が抜歯後歯根を破折せず再植した群より有意に小さかった. 4)インパーバデュアル,ビスタイトレジンセメントを使用した群は,スーパーボンドC&B群とパナビア 21群の中間的な状態を示した
• Treatment of Vertically Fractured Root by Cohesion Part 1. Evaluations of Cytotoxicity of Adhesive Resin Cement with Cultured Periodontal Ligament Cells
  NOGUCHI Hiroshi; SUGAYA Tsutomu; KATO Hiroshi
  Jpn J Conserv Dent, 40, 6, 1445, 1452, (NPO)日本歯科保存学会, 01 Dec. 1997, [Peer-reviewed]
  Japanese, 1)スーパーボンドC&B,インパーバデュアル,ビスタイトレジンセメントは,ヒト培養歯根膜細胞の付着・増殖が,root planingした根面とほぼ同等であったことから,この3種の接着性レジンセメントが培養歯根膜細胞に与える影響は僅かである. 2)この3種の接着性レジンセメントの中では,スーパーボンドC&Bが最も細胞の付着・増殖が多かった. 3)パナビア 21には,培養歯根膜細胞の付着・増殖がみられなかったが,近接する培養歯根膜細胞への影響は小さかった
• A Study of the Preventive Program for Periodontal Disease in Association with Adult Mass Dental Examination II An Evaluation of the Preventive Program by Using Hayashi's Quantification
  WATABE Nobuki; SUGAYA Tsutuomu; MUKAINAKANO Yutaka; KATO Hiroshi
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 38, 4, 529, 537, The Japanese Society of Periodontology, 28 Dec. 1996, [Peer-reviewed]
  Japanese, In order to establish a preventive program for periodontal disease in association with adult mass dental examination, we surveyed the employees of 14 companies in Hokkaido from the previous study. According to the CPITN-Max value, the preventive measures for periodontal disease were recorded including oral hygiene instruction, scaling and recommendation to a dental office.
  The purpose of this study was to evaluate the effectiveness of our preventive program for periodontal disease by using Hayashi's Quantification type II. This preventive program was performed during and after mass dental examination. The same subjects were re-examined after 1.5 to 2 years in a follow-up examination.
  The results were as follows:
  1. At the first examination, 187 out of 225 subjects were found to require preventive measures, but only 111 subjects (59.4%) received them.
  2. Our preventive measures should be adopted during mass dental examination, because some subjects who were contacted subsequently failed to come to receive our preventive measures.
  3. CPITN was significantly improved by this preventive program for periodontal disease.
  4. Hayashi's Quantification analysis indicated that scaling was the most effective factor in improving the CPITN value.
  5. One time oral hygiene instruction alone at the first examination had little effect.
  6. Recommendation to visiting a dental office had little effect.
• A Study of the Preventive Program for Periodontal Disease in Association with Adult Mass Dental Examination I Examination of the Preventive Program According to CPITN
  SUGAYA Tsutomu; MUKAINAKANO Yutaka; WATABE Nobuki; KATO Hiroshi
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 38, 4, 522, 527, The Japanese Society of Periodontology, 28 Dec. 1996, [Peer-reviewed]
  Japanese, Scientific journal, The purpose of this study was to establish a preventive program for periodontal disease associated with adult mass dental examination. Periodontal status was surveyed by using CPITN among 900 employees (mean age: 41.2) in Hokkaido. A questionnaire was given to 411 of the subjects. The questionnaire included smoking habits, personal awareness of periodontal disease, history of periodontal treatments and habits of oral hygiene. The influence of these factors, PH. and C. I. on CPITN was analyzed by using Hayashi's Quantification to determine the essential factors of the preventive program and to evaluate the possibility of selecting a high risk group from the questionnaire, who may require intensive preventive measures. The following results were obtained. The prevalence of periodontal disease was 84.5%. 82.2% of subjects showed a CPITN of more than 2. Therefore it was suggested that oral hygiene instruction alone was ineffective and that scaling was neccesary for the prevention of periodontal disease. A significant correlation was found between CPITN and oral hygiene index, while smoking habits and other factors had little influence. Selection of a high risk group from the quesionnaire was not possible. These results suggested that a preventive program of periodontal disease combined with adult mass dental examination should be performed in all subjects irrespective of other factors (ie., awareness of periodontal disease and oral hygiene habits) and preventive measures (ie., oral hygiene instruction, scaling and recommendation to a dental office) should be taken according to periodontal status.
• Regeneration of the Periodontal Ligament and Cementum by Implantation of Cultured Periodontal Ligament Cells -Histological Evaluation of Transplanted Dentin Blocks with Cultured Periodontal Ligament Cells-
  OHMORI Mikio; SUGAYA Tsutomu
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 38, 4, 447, 456, The Japanese Society of Periodontology, 28 Dec. 1996, [Peer-reviewed]
  Japanese, Scientific journal, This study evaluated a new regenerative approach for the periodontal ligament and cementum by implantation of cultured periodontal ligament cells.
  The study was performed with syngeneic rats divided into the following three groups. In the experimental group, dentin blocks with cultured periodontal ligament cells were transplanted to the cranial bone defect. In the control group, dentin blocks without cells were transplanted. In the tooth root transplantation group, extracted teeth with fresh periodontal ligament from other rats were transplanted. In all groups, the cranial tissues were routinely prepared for the histological analyses at 2, 4 and 6 weeks after transplantation.
  In the experimental group, formation of cementum-like tissue was observed on 1 of 6 blocks at 2 weeks and 4 of 10 blocks at 4 weeks and dentin blocks showed ankylosis at 6 weeks after transplantation. In the control group, dentin blocks partly exhibited ankylosis at 2 weeks and all blocks exhibited ankylosis at 4 and 6 weeks after transplantation. In the tooth root transplantation group, periodontal ligament had established at 2 weeks, partial root resorption was observed at 4 weeks and dentin blocks exhibited obvious ankylosis at 6 weeks after transplantation.
  These results suggested that the implantation of cultured periodontal ligament cells had the regenerative potential of the periodontal ligament and cementum. But even in the experimental group, dentin blocks exhibited ankylosis in all cases at six weeks after transplantation, that was similar to the tooth root transplantation group. This suggests that an occlusal function or similar kinds of function may be involved in the process of regeneration after implantation.
• Influence of Application Conditions of Ultrasonic Scaler with Probe-type Tip on the Morphology and Character of Root Surfaces.
  SUGAYA Tsutomu
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 35, 4, 577, 586, The Japanese Society of Periodontology, 1993, [Peer-reviewed]
  Japanese, The purpose of this study was to examine the influence of application conditions of an ultrasonic scaler with a probe-type tip on the morphology and character of root surfaces. Firstly, dentine specimens were root planed under 12 conditions with an ultrasonic probe-type scaler, then the depths of tooth surface defects and roughness were measured with a profilometer and morphology was examined by SEM. Secondly, fibroblasts were cultured on periodontally diseased root surfaces after root planing under the same conditions. The number of attached and grown cells were counted under light microscopy. The results show that the degree of defects, roughness and the removal of cytotoxic substances increased when the power setting was higher, the applied pressure increased and the vibrating direction was vertical to the root surface. The results reveal that the effectiveness of the ultrasonic scaler with a probe-type tip under proper conditions is equal to that of a hand scaler in terms of root surface smoothness and the removal of cytotoxic substances.
• A newly designed probe-type tip for ultrasonic scaling. Clinical evaluation after subgingival root debridement.:Clinical Evaluation after Subgingival Root Debridement
  IINUMA Kenji; SUGAYA Tsutomu; TATE Takako; KAWANAMI Masamitsu; KATO Seiya; KATO Hiroshi
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 30, 3, 926, 934, The Japanese Society of Periodontology, 1988, [Peer-reviewed]
  Japanese, The purpose of this study was to investigate clinically the healing events of periodontitis after subgingival debridement with an ultrasonic probe-type tip scaler. 213 surfaces of 74 non-instrumented teeth with periodontitis in 12 patients were studied. All subjects received oral hygiene instruction and supragingival scaling. After each patient was found to maintain a good oral hygiene condition, the experimental period began. As a baseline, ultrasonic subgingival debridement with the newly designed device was performed on 128 experimental root surfaces until the operator was convinced of completing the debridement, and the remaining 85 surfaces served as a non-instrumented control. Plaque index, probing depth, clinical attachment level, gingival crevicular fluid and bleeding on probing were evaluated at the baseline and 1, 2 and 4 weeks after instrumentation.
  The results were as follows: The mean plaque index remained constantly low in both experimental and control groups. Inflammatory indicies (bleeding on probing and gingival crevicular fluid) were markedly reduced in the experimental group. Experimental sites with initial probing depth of . 4 mm showed marked reduction in probing depth and an increase in attachment level although those parameters in the control sites remained unchanged.
  The results demonstrate that subgingival debridement with the new device is efficient for periodontal treatment in patients with good oral hygiene.
• ポケット探針型チップを応用した超音波スケーラーによる歯肉縁下歯垢歯石の除去効果について : 第2報 深い歯周ポケットでの清掃能力について : 第30回春季日本歯周病学会総会
  菅谷 勉; 川浪 雅光; 加藤 誠也; 国立 尊子; 飯沼 賢司; 加藤 熈
  日本歯周病学会会誌, 29, 3, 1006, 1006, 特定非営利活動法人日本歯周病学会, 28 Sep. 1987, [Peer-reviewed]
  Japanese
• Subgingival ultrasonic debridement with newly designed probe-typed tip. I. Accessibility into the bottom of deep periodontal pocket.:I. Accessibility into the Bottom of Deep Periodontal Pocket
  KATO Seiya; SUGAYA Tsutomu; KAWANAMI Masamitsu; KUNITACHI Takako; IINUMA Kenji; KATO Hiroshi
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 29, 2, 627, 633, The Japanese Society of Periodontology, 1987, [Peer-reviewed]
  Japanese, Removal of supra and subgingival plaque and calclus is the most important factor in periodontal therapy. In practice, however, deeper the pocket is, the harder is to make the instrumental approach with hand or conventional ultrasonic scaler due to the registance of the soft tissue wall of the pocket.
  Periodontal probe is readily accessible to the bottom of the pocket with slight patient's discomfort without local anesthesia. We designed probe-typed tip for ultrasonic scaler (OSADA ENAC-3) capable of being used for debridement in the deep pocket.
  To examine the functional potential of the device, in vitro and in vivo experiments were undertaken in the present study. In the first experiment effect of the tip on removing 170 calculi spots on extracted teeth roots was evaluated. The second was carried out on 46 root surfaces of 30 teeth scheduled for extraction due to advanced periodontal disease. To examine the accessibility of the tip to the bottom of the pockt, its ability to remove the plaque and calculus and patient's discomfort during the procedure, ultrasonic debridement with the tip was done in linear fassion along the long-axis of selected tooth surface to the bottom of the pocket. The tooth was extracted and stained with 1% methylene blue. The depth of pocket and ultrasonic debridement area were measured under stereo-microscope. Furthermore, the patient's discomfort while debridement procedure with the tip was also evaluated.
  Following results were obtained.
  1. The device could efficiently remove all the calculi spots on extracted teeth in vitro study.
  2. Accessibility and plaque and calculus removability of the tip was good in patients with less than 9 mm depth pocket.
  3. In pockets over apical curved roots, acces-sibility for debridement was hard to accomplish.
  4. While ultrasonic debridement, 69.2% of the patients did not complain of discomfort, 28.2% claimed slight discomfort and only 2.6% needed local anesthesia who had hypersensitivity against probing before ultrasonic debridement.
• Subgingival ultrasonic debridement with newly designed probe-type tip. Ability to remove subgingival plaque and calculus in deep periodontal pocket.:Ability to Remove Subgingival Plaque and Calculus in Deep Periodontal Pocket
  SUGAYA Tsutomu; KAWANAMI Masamitsu; KATO Seiya; KUNITATE Takako; IINUMA Kenji; KATO Hiroshi
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 29, 3, 959, 965, The Japanese Society of Periodontology, 1987, [Peer-reviewed]
  Japanese, Destructive periodontal disease is closely associated with subgingival plaque and calculus. Probe-type tip was newly designed for ultrasonic scaler (OSADA ENAC-3) capable of being used for easy and effective debridement in the deep pocket. In our previous study, the tip was found to access easily into the bottom of the deep periodontal pocket without local anesthesia.
  The purpose of the present study was to examine the ability of this device to remove subgingival plaque and calculus in deep periodontal pocket.
  Eighty-eight surfaces of 43 non-instrumented teeth with advanced periodontitis scheduled for extraction were used. Ultrasonic debridement was done on 58 experimental root surfaces until the operator was convinced. During the procedure patient's discomfort and operating time was recorded. Thirty surfaces were non-instrumented control. All teeth were extracted and stained with 1% Methylene blue to distinguish residual plaque and calculus. Color slides were prepared for quantitative analysis. Pocket area and residual plaque and calculus area were calculated by the use of a graphic analizing system in the personal computer on 10 times image of the teeth.
  Following results were obtained. The mean percentage of residual plaque and calculus area of the experimental surfaces was 4.6% and the percentage of control surfaces was 94.3%. No Significant difference was found in the above percentage of experimental surfaces between anterior or posterior.
  When the pocket depth was less than 8mm, there seemed to be no difficulty to remove plaque and calculus in the pockets regardless of the depth of pocket. But when the pocket depth was more than 8mm, complete removal in the bottom of the pocket was occasionally difficult. In those cases, as the bottom of the pocket was one the distinctly curved apex, so the tip was inaccessible. Ultrasonic debridement was done without local anesthesia, but none of the patients complained any unbearable pain while the procedure.
• The effect of mouth breathing as an etiologic factor of periodontal disease. Dehydration measured in palate using three-dimensional artificial mouth-model.:Dehydration Measured in Palate Using Three-Dimensional Artificial Mouth-Model
  DOHKAI Hitoshi; WATANABE Tomoko; SUGAYA Tsutomu; TOUKAIRIN Kouji; ISHIKAWA Jun; KATOH Hiroshi; YOSHIDA Shizuo
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 29, 3, 853, 859, The Japanese Society of Periodontology, 1987, [Peer-reviewed]
  Japanese, The present experiment was undertaken to evaluate the association and the effect of mouth breathing in the initiation and the progression of periodontal disease. Artificial mouth model-fulfilling nearly all the characteristics of natural mouth was made and dehydration in the palatal region was measured.
  The components of three dimentional artificial model was consisted of plastic plate for upper jaw, hard plaster for lower jaw and white plaster for the tongue. Mouth to pharynx unidirectional air flow was established in the model by electric vaccum system. Ten points were selected in the palate to measure dehydration. Constant air flow maintaining the volume of human inspiratory (500ml/sec.) was continued for 25 minutes and dehydration was measured at the individual selected points.
  As a result, the maximum dehydration was recorded at the palatal gum-margin of upper central incisor, next was that of upper lateral incisor and canine. Minimum dehydration was recorded at the center of the palate and gum-margin of premolars.
  The region of maximum dehydration in the artificial mouth-model was coincident to the area of tension ridge (upper anterior), suggesting that mouth breathing may be one of the important etiologic factor of periodontal disease.

• リン酸化プルラン含有MTAの逆根管充填における封鎖性と歯根端切除術後の治癒に対する有効性
  堂 寛隆; 辻村 大河; 佐藤 賢人; 川上 紗也雅; 高橋 直紀; 吉田 靖弘; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 164回, 133, 133, May 2026
  (NPO)日本歯科保存学会, Japanese
• リン酸化プルラン-CaCl2-βTCP-BMP2複合体移植の歯槽提増大への有効性
  辻村 大河; 菅谷 勉; 高橋 直紀; 堂 寛隆; 川上 紗也雅; 沼田 沙和; 町田 紗樹; 櫻井 直人; 兵藤 佑貢真; 赤坂 司; 吉田 靖弘; 中西 康; 林 宏至, 日本歯周病学会会誌, 67, 秋季特別, 157, 157, Oct. 2025
  (NPO)日本歯周病学会, Japanese
• リン酸化プルラン-CaCl2-βTCP-BMP2複合体移植のラット頭蓋骨上への移植による骨形成
  原田 天乃; 菅谷 勉; 高橋 直紀; 堂 寛隆; 兵藤 佑貢真; 櫻井 直人; 川上 紗也雅; 沼田 沙和; 町田 紗樹, 日本歯周病学会会誌, 67, 秋季特別, 158, 158, Oct. 2025
  (NPO)日本歯周病学会, Japanese
• 垂直破折歯根の診断と長期治療成績および予後因子
  菅谷 勉, 日本補綴歯科学会誌, 17, 4, 195, 200, Oct. 2025
  (公社)日本補綴歯科学会, Japanese
• 根尖孔外開き根管への高周波通電による焼灼
  明本 菜穂; 野村 卓巳; 高橋 直紀; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 163回, 167, 167, Oct. 2025
  (NPO)日本歯科保存学会, Japanese
• 根尖病変を有する穿通不可根管における高周波通電の有用性
  富永 敏彦; 高比良 一輝; 加藤 寛二郎; 木村 祐介; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 163回, 179, 179, Oct. 2025
  (NPO)日本歯科保存学会, Japanese
• 口腔清掃状態が低下した歯周炎患者への洗口剤と繰り返しのスケーリングによるプラークコントロール
  菅谷 勉, 日本歯周病学会会誌, 67, 秋季特別, 133, 133, Oct. 2025
  (NPO)日本歯周病学会, Japanese
• リン酸化プルラン-CaCl2-βTCP-BMP2複合体移植の歯槽提増大への有効性
  辻村 大河; 菅谷 勉; 高橋 直紀; 堂 寛隆; 川上 紗也雅; 沼田 沙和; 町田 紗樹; 櫻井 直人; 兵藤 佑貢真; 赤坂 司; 吉田 靖弘; 中西 康; 林 宏至, 日本歯周病学会会誌, 67, 秋季特別, 157, 157, Oct. 2025
  (NPO)日本歯周病学会, Japanese
• リン酸化プルラン-CaCl2-βTCP-BMP2複合体移植のラット頭蓋骨上への移植による骨形成
  原田 天乃; 菅谷 勉; 高橋 直紀; 堂 寛隆; 兵藤 佑貢真; 櫻井 直人; 川上 紗也雅; 沼田 沙和; 町田 紗樹, 日本歯周病学会会誌, 67, 秋季特別, 158, 158, Oct. 2025
  (NPO)日本歯周病学会, Japanese
• 抜髄処置における高周波通電の有効性
  富永 敏彦; 高比良 一輝; 多田 瑛一朗; 加藤 寛二郎; 菅谷 勉, 日本歯内療法学会雑誌, 46, 3, 226, 235, Sep. 2025
  (一社)日本歯内療法学会, Japanese
• 器具到達不可根管の焼灼に対する根管の長さと高周波通電回数の影響
  白石 司; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 162回, 129, 129, Jun. 2025
  (NPO)日本歯科保存学会, Japanese
• 【歯の移植・再植Q&A 天然歯の有効利用からトラブル回避まで】(第3章)再植 症例選択(Q.17) どのような症例で意図的再植を行えばよいでしょうか。
  菅谷 勉, DENTAL DIAMOND, 50, 6, 90, 96, Apr. 2025
  (株)デンタルダイヤモンド社, Japanese
• 【歯の移植・再植Q&A 天然歯の有効利用からトラブル回避まで】(第3章)再植 症例選択(Q.19) 再植可能な垂直破折歯の基準を教えてください。
  菅谷 勉, DENTAL DIAMOND, 50, 6, 102, 108, Apr. 2025
  (株)デンタルダイヤモンド社, Japanese
• 塩基性線維芽細胞増殖因子の担体にリン酸化プルランを用いて皮質骨穿孔部にOnlay graftした場合の骨造成効果
  竹中 裕喜; 堂 寛隆; 川上 紗也雅; 櫻井 直人; 兵藤 佑貢真; 菅谷 勉, 日本歯周病学会会誌, 67, 春季特別, 161, 161, Apr. 2025
  (NPO)日本歯周病学会, Japanese
• 耳鼻咽喉科医が知っておきたい歯科疾患
  菅谷 勉, 日本耳鼻咽喉科頭頸部外科学会会報, 128, 1, 66, 69, Jan. 2025
  (一社)日本耳鼻咽喉科頭頸部外科学会, Japanese
• 到達不可根管に対する高周波通電時の電極位置と時間が根尖性歯周炎の改善におよぼす影響(The Influence of Electrode Positioning and Duration during High-Frequency Current Application on Improvement of Apical Periodontitis in case of Inaccessible Root Canal)
  黄 宗楷; 佐藤 賢人; 竹中 裕喜; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 161回, 28, 28, Nov. 2024
  (NPO)日本歯科保存学会, English
• 到達不可根管への高周波通電による細菌の焼灼および除去効果
  佐久間 柚衣; 菅谷 勉; 長谷部 晃, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 161回, 98, 98, Nov. 2024
  (NPO)日本歯科保存学会, Japanese
• 垂直破折歯根の長期治療成績と予後因子
  菅谷 勉, 日本補綴歯科学会誌, 16, 特別号, 131, 131, Jul. 2024
  (公社)日本補綴歯科学会, Japanese
• ルートZX3とJIZAI,メタシールSoftペーストを活用した簡単で好成績の根管治療
  菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 160回, 29, 29, Apr. 2024
  (NPO)日本歯科保存学会, Japanese
• Characterization and Biocompatibility Evaluation of Zinc Oxide Eugenol-based Paste Root Canal Sealer
  加藤昭人; 宮治裕史; 金本佑生実; 蔀佳奈子; 岡本一絵; 吉野友都; 浜本朝子; 西田絵利香; 菅谷勉; 田中佐織, 日本歯科保存学雑誌(Web), 66, 2, 2023
• ヒトI型コラーゲン様リコンビナントペプチド/β-三リン酸カルシウム骨補填材による歯周組織再生効果
  吉野友都; 宮治裕史; 西田絵利香; 金本佑生実; 浜本朝子; 加藤昭人; 菅谷勉; 赤坂司, 日本歯周病学会会誌(Web), 65, 2023
• 全身性疾患への影響を考慮した新たな歯周病重症度検査項目の策定~学会主導型多施設臨床研究~第二報
  松田真司; 菅谷勉; 加藤幸紀; 根本英二; 竹内康雄; 山下慶子; 沼部幸博; 西田哲也; 小方頼昌; 申基哲; 長野孝俊; 両角俊哉; 小松康高; 出分菜々衣; 後藤亮真; 北村正博; 田口洋一郎; 高柴正悟; 湯本浩通; 山下明子; 吉永泰周; 吉村篤利; 河口浩之, 日本歯周病学会会誌(Web), 65, 2023
• Surface pre-reacted glass-ionomer(S-PRG)ナノフィラー分散液を注水に用いた超音波スケーラーによる歯周炎改善効果
  宮治 裕史; 眞弓 佳代子; 金本 佑生実; 岡本 一絵; 浜本 朝子; 加藤 昭人; 菅谷 勉; 赤坂 司; 田中 佐織, 日本歯周病学会会誌, 64, 秋季特別, 126, 126, Aug. 2022
  (NPO)日本歯周病学会, Japanese
• 前駆体利用バイオミメティックプロセスによる低結晶性アパタイト成膜足場材 骨・歯周組織形成能の評価
  金本 佑生実; 宮治 裕史; 西田 絵利香; 宮田 さほり; 眞弓 佳代子; 吉野 友都; 加藤 昭人; 菅谷 勉; 赤坂 司; 大矢根 綾子, 日本歯周病学会会誌, 64, 秋季特別, 122, 122, Aug. 2022
  (NPO)日本歯周病学会, Japanese
• Surface pre-reacted glass-ionomer(S-PRG)フィラー配合水硬性仮封材の抗菌特性
  宮治 裕史; 金本 佑生実; 菅谷 勉; 田中 佐織, 日本歯内療法学会学術大会プログラム・抄録集, 43回, 61, 61, Jul. 2022
  (一社)日本歯内療法学会, Japanese
• 接着性シーラーを用いた根管充填が垂直歯根破折の抵抗性に及ぼす効果
  河合 大史; 鷲巣 太郎; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 156回, 50, 50, May 2022
  (NPO)日本歯科保存学会, Japanese
• 歯ブラシ・歯磨剤・洗口剤の理解を深めるための体感型授業
  田中 佐織; 菅谷 勉; 西川 昌弘, 日本歯周病学会会誌, 64, 春季特別, 124, 124, May 2022
  (NPO)日本歯周病学会, Japanese
• 酸化グラフェン超薄膜コーティングへの口腔洗浄含嗽剤の複合化による持続性抗菌効果
  宮治 裕史; 金本 佑生実; 浜本 朝子; 西田 絵利香; 加藤 昭人; 菅谷 勉; 田中 佐織; 郷田 隼; 小野 博信, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 156回, 89, 89, May 2022
  (NPO)日本歯科保存学会, Japanese
• 臼歯部咬頭干渉による咬合性外傷に咬合再構成で対応した慢性歯周炎症例
  宮治 裕史; 菅谷 勉, 日本歯周病学会会誌, 64, 春季特別, 137, 137, May 2022
  (NPO)日本歯周病学会, Japanese
• Effect of different root canal filling techniques with methacrylic acid ester adhesive sealer in wet inaccessible root canals on quality of root canal obturation
  中鉢奈都子; 原田天乃; 本郷裕美; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 157th, 2022
• In Vivo Inflammatory Effects and Surface Composition Changes in Implanted Root Canal Sealer Containing Bioactive Glass
  加藤昭人; 宮治裕史; 吉野友都; 金本佑生実; 浜本朝子; 西田絵利香; 菅谷勉; 田中佐織; 田中佐織, 日本歯科保存学雑誌(Web), 65, 2, 2022
• 全身性疾患への影響を考慮した新たな歯周病重症度検査項目の策定~学会主導型多施設臨床研究~
  松田真司; 菅谷勉; 加藤幸紀; 根本英二; 竹内康雄; 喜田大智; 沼部幸博; 西田哲也; 小方頼昌; 申基哲; 長野孝俊; 両角俊哉; 小松康高; 出分菜々衣; 神谷洋介; 北村正博; 田口洋一郎; 高柴正悟; 湯本浩通; 山下明子; 吉永泰周; 吉村篤利; 河口浩之, 日本歯周病学会会誌(Web), 64, 2022
• Antibacterial ability of hydraulic temporary sealing material containing surface pre-reacted glass-ionomer (S-PRG) fillers
  宮治裕史; 金本佑生実; 菅谷勉; 田中佐織; 田中佐織, 日本歯内療法学会学術大会プログラム・抄録集, 43rd, 2022
• 歯ブラシ・歯磨剤・洗口剤の理解を深めるための体感型授業
  田中佐織; 菅谷勉; 西川昌弘, 日本歯周病学会会誌(Web), 64, 2022
• 前駆体利用バイオミメティックプロセスによる低結晶性アパタイト成膜足場材-骨・歯周組織形成能の評価-
  金本佑生実; 宮治裕史; 西田絵利香; 宮田さほり; 眞弓佳代子; 吉野友都; 加藤昭人; 菅谷勉; 赤坂司; 大矢根綾子, 日本歯周病学会会誌(Web), 64, 2022
• 臼歯部咬頭干渉による咬合性外傷に咬合再構成で対応した慢性歯周炎症例
  宮治裕史; 菅谷勉, 日本歯周病学会会誌(Web), 64, 2022
• 歯周病のポケット検査における代表歯・部位の選定
  両角 俊哉; 高柴 正悟; 三邉 正人; 野村 義明; 福田 光男; 花田 信弘; 角田 衣理加; 小林 宏明; 中村 利明; 中山 洋平; 西村 英紀; 野口 和行; 沼部 幸博; 小方 頼昌; 齋藤 淳; 佐藤 聡; 関野 愉; 菅野 直之; 菅谷 勉; 鈴木 史彦; 多部田 康一; 高橋 慶壮; 高井 英樹; 梅田 誠; 吉村 篤利; 吉成 伸夫; 中川 種昭, 日本口腔検査学会総会・学術大会プログラム・抄録集, 14回, 70, 71, Aug. 2021
  (一社)日本口腔検査学会, Japanese
• 歯周病評価における最適検査部位の選定 項目反応理論Graded response modelの応用
  両角 俊哉; 野村 義明; 福田 光男; 花田 信弘; 角田 衣理加; 小林 宏明; 三邉 正人; 中村 利明; 中山 洋平; 西村 英紀; 野口 和行; 沼部 幸博; 小方 頼昌; 齋藤 淳; 佐藤 聡; 関野 愉; 菅野 直之; 菅谷 勉; 鈴木 史彦; 多部田 康一; 高橋 慶壮; 高井 英樹; 高柴 正悟; 梅田 誠; 吉江 弘正; 吉村 篤利; 吉成 伸夫; 中川 種昭, 日本歯周病学会会誌, 63, 春季特別, 106, 106, May 2021
  (NPO)日本歯周病学会, Japanese
• 歯周病評価における最適検査部位の選定 項目反応理論Graded response modelの応用
  両角 俊哉; 野村 義明; 福田 光男; 花田 信弘; 角田 衣理加; 小林 宏明; 三邉 正人; 中村 利明; 中山 洋平; 西村 英紀; 野口 和行; 沼部 幸博; 小方 頼昌; 齋藤 淳; 佐藤 聡; 関野 愉; 菅野 直之; 菅谷 勉; 鈴木 史彦; 多部田 康一; 高橋 慶壮; 高井 英樹; 高柴 正悟; 梅田 誠; 吉江 弘正; 吉村 篤利; 吉成 伸夫; 中川 種昭, 日本歯周病学会会誌, 63, 春季特別, 106, 106, May 2021
  (NPO)日本歯周病学会, Japanese
• 酸化グラフェン/塩化セチルピリジニウム複合超薄膜による歯面コーティングと抗菌評価
  宮治裕史; 浜本朝子; 西田絵利香; 菅谷勉; 蔀佳奈子; 郷田隼; 小野博信, 日本歯周病学会会誌(Web), 63, 2021
• 骨補填材としてのリン酸化プルラン/β-TCPコンビネーションマテリアルの応用
  森本康仁; 久保田恵亮; 阿部未来; 丸岡春日; 長谷川智香; 本郷裕美; 吉田靖弘; 網塚憲生; 菅谷勉, 日本歯周病学会会誌(Web), 63, 2021
• S-PRGナノフィラーを用いた抗菌性歯面コーティングによる臨床パラメーターの改善-イヌ実験的歯周炎における検討-
  眞弓佳代子; 宮治裕史; 宮田さほり; 西田絵利香; 降旗友和; 金本佑生実; 菅谷勉; 蔀佳奈子; 赤坂司, 日本歯周病学会会誌(Web), 63, 2021
• リゾチーム-金ナノクラスター/ローズベンガル複合体の抗菌・抗バイオフィルム光線力学的活性
  岡本一絵; 宮治裕史; 宮田さほり; 菅谷勉; 蔀佳奈子; 赤坂司; 齋田慧; 川崎英也, 日本歯周病学会会誌(Web), 63, 2021
• Preparation and antibacterial evaluation of graphene oxide/cationic surfactant ultrathin films.
  宮治裕史; 浜本朝子; 西田絵利香; 金本佑生実; 菅谷勉; 蔀佳奈子; 郷田隼; 小野博信, 日本バイオマテリアル学会大会予稿集(Web), 43rd, 2021
• リン酸三カルシウム(TCP)およびリン酸化プルラン(PPL)含有新規骨補填材を用いた骨再生
  森本康仁; 長谷川智香; 本郷裕美; 久保田恵亮; 阿部未来; 丸岡春日; 吉田靖弘; 菅谷勉; 網塚憲生, 日本再生医療学会総会(Web), 20th, 2021
• リン酸化プルラン/β-TCPコンビネーションマテリアルにおける新規骨再生誘導
  森本康仁; 森本康仁; 久保田恵亮; 阿部未来; 丸岡春日; 本郷裕美; 吉田靖弘; 菅谷勉; 網塚憲生; 長谷川智香, 日本骨形態計測学会雑誌, 31, 2, 2021
• Elemental composition analysis of root canal sealer containing bioactive glass implanted into rat subcutaneous tissue
  宮治裕史; 吉野友都; 加藤昭人; 菅谷勉; 田中佐織; 田中佐織, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 154th, 2021
• 過飽和液中レーザー照射法による象牙質表面へのフッ素担持アパタイト成膜と抗菌特性
  蔀 佳奈子; 宮治 裕史; 菅谷 勉; 古賀 健司; 中村 真紀; 大矢根 綾子, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 152回, 80, 80, Jun. 2020
  (NPO)日本歯科保存学会, Japanese
• Bioactive Glass配合根管充填シーラーの生体親和性評価
  吉野 友都; 宮治 裕史; 金本 佑生実; 浜本 朝子; 西田 絵利香; 加藤 昭人; 菅谷 勉; 田中 佐織, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 152回, 103, 103, Jun. 2020
  (NPO)日本歯科保存学会, Japanese
• 高周波電流によるファイル未到達根管内容物の焼灼
  内沢 英作; 熊谷 広道; 百海 啓; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 152回, 134, 134, Jun. 2020
  (NPO)日本歯科保存学会, Japanese
• 金銀クラスター/ローズベンガル含有キトサンナノゲル複合体の光線力学的特性
  浜本 朝子; 加藤 昭人; 宮田 さほり; 蔀 佳奈子; 宮治 裕史; 菅谷 勉; 齋田 慧; 川崎 英也, 北海道外科雑誌, 65, 1, 91, 91, Jun. 2020
  北海道外科学会, Japanese
• リン酸化プルラン含有根管充填シーラーの封鎖性と根尖病巣の治療効果
  大嶌 理紗; 河野 通俊; 下地 伸司; 菅谷 勉; 吉田 靖弘, 北海道外科雑誌, 65, 1, 91, 92, Jun. 2020
  北海道外科学会, Japanese
• ブラッシングが低下した歯周炎患者のプラークコントロール
  菅谷 勉, 特定非営利活動法人日本臨床歯周病学会年次大会プログラム・講演抄録集, 38回, 88, 88, Jun. 2020
  (NPO)日本臨床歯周病学会, Japanese
• 難治性根尖性歯周炎の治療
  菅谷 勉, 北海道歯学雑誌, 40, 2, 129, 129, Mar. 2020
  北海道歯学会, Japanese
• 歯の再植・垂直歯根破折・剥離性歯根破折から考える歯根膜の役割
  菅谷 勉, 日本臨床歯周病学会会誌, 37, 2, 11, 16, Feb. 2020
  (NPO)日本臨床歯周病学会, Japanese
• リン酸三カルシウム(TCP)/リン酸化プルラン(PPL)含有新規骨補填材による新生骨形成誘導について
  本郷裕美; 森本康仁; 森本康仁; 長谷川智香; 菅谷勉; 吉田靖弘; 網塚憲生, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 125th, 2020
• リン酸三カルシウム(TCP)/リン酸化プルラン(PPL)含有新規骨補填材によって形成された新生骨の組織学的検索
  本郷裕美; 長谷川智香; 森本康仁; 森本康仁; 菅谷勉; 吉田靖弘; 網塚憲生, 日本再生医療学会総会(Web), 19th, 2020
• メタシールSoftを用いた根管充填後の臨床成績に関する後ろ向き研究
  金子 至; 内川 宗敏; 松井 力; 汲田 剛; 三溝 泰弘; 丸山 慶四郎; 菅谷 勉, 日本歯科保存学雑誌, 62, 6, 279, 285, Dec. 2019
  (NPO)日本歯科保存学会, Japanese
• リン酸三カルシウム(TCP)およびリン酸化プルラン(PPL)含有新規骨補填材の開発
  森本 康仁; 長谷川 智香; 本郷 裕美; 吉田 靖弘; 網塚 憲生; 菅谷 勉, 日本歯周病学会会誌, 61, 秋季特別, 142, 142, Oct. 2019
  (NPO)日本歯周病学会, Japanese
• 新規メタクリル酸エステル系接着性シーラーの硬化と封鎖性に及ぼす水の影響
  鈴木 魁; 鷲巣 太郎; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 151回, 120, 120, Oct. 2019
  (NPO)日本歯科保存学会, Japanese
• 高周波電流による歯髄の焼灼
  森 厚志; 河野 通俊; 中谷 充宣; 前田 良子; 降籏 友和; 中塚 愛; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 151回, 125, 125, Oct. 2019
  (NPO)日本歯科保存学会, Japanese
• 高周波通電による未到達領域における歯根温度変化
  多田 瑛一朗; 富永 敏彦; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 151回, 126, 126, Oct. 2019
  (NPO)日本歯科保存学会, Japanese
• 高周波電流によるファイル未到達根管の焼灼
  熊谷 広道; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 151回, 127, 127, Oct. 2019
  (NPO)日本歯科保存学会, Japanese
• β-TCPナノ粒子配合ヒトI型コラーゲン様リコンビナントペプチド骨補填材による骨形成効果
  降籏 友和; 宮治 裕史; 西田 絵利香; 加藤 昭人; 宮田 さほり; 蔀 佳奈子; 眞弓 佳代子; 金本 佑生実; 菅谷 勉; 赤坂 司, 日本歯周病学会会誌, 61, 秋季特別, 126, 126, Oct. 2019
  (NPO)日本歯周病学会, Japanese
• ナノS-PRGフィラー分散液と超音波スケーラー注水システムによる抗菌性歯面の構築 フィラーの歯面付着性の検討
  眞弓 佳代子; 宮治 裕史; 岡本 一絵; 加藤 昭人; 菅谷 勉, 日本歯周病学会会誌, 61, 秋季特別, 143, 143, Oct. 2019
  (NPO)日本歯周病学会, Japanese
• キャナルスペーストチューブおよびキャナルスペーストダブルシリンジの抗菌性ならびに生体親和性評価
  金本 佑生実; 宮治 裕史; 蔀 佳奈子; 岡本 一絵; 吉野 友都; 浜本 朝子; 菅谷 勉; 田中 佐織, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 151回, 119, 119, Oct. 2019
  (NPO)日本歯科保存学会, Japanese
• リン酸三カルシウム(TCP)およびリン酸化プルラン(PPL)含有新規骨補填材の開発
  森本康仁; 長谷川智香; 本郷裕美; 吉田靖弘; 網塚憲生; 菅谷勉, 日本歯周病学会会誌(Web), 61, 142, 01 Oct. 2019
  Japanese
• β-TCPナノ粒子配合ヒトI型コラーゲン様リコンビナントペプチド骨補填材による骨形成効果
  降籏 友和; 宮治 裕史; 西田 絵利香; 加藤 昭人; 宮田 さほり; 蔀 佳奈子; 眞弓 佳代子; 金本 佑生実; 菅谷 勉; 赤坂 司, 日本歯周病学会会誌, 61, 秋季特別, 126, 126, Oct. 2019
  (NPO)日本歯周病学会, Japanese
• ナノS-PRGフィラー分散液と超音波スケーラー注水システムによる抗菌性歯面の構築 フィラーの歯面付着性の検討
  眞弓 佳代子; 宮治 裕史; 岡本 一絵; 加藤 昭人; 菅谷 勉, 日本歯周病学会会誌, 61, 秋季特別, 143, 143, Oct. 2019
  (NPO)日本歯周病学会, Japanese
• キャナルスペーストチューブおよびキャナルスペーストダブルシリンジの抗菌性ならびに生体親和性評価
  金本 佑生実; 宮治 裕史; 蔀 佳奈子; 岡本 一絵; 吉野 友都; 浜本 朝子; 菅谷 勉; 田中 佐織, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 151回, 119, 119, Oct. 2019
  (NPO)日本歯科保存学会, Japanese
• β‐TCPナノ粒子配合ヒトI型コラーゲン様リコンビナントペブチド骨補填材による骨形成効果
  降籏友和; 宮治裕史; 西田絵利香; 加藤昭人; 宮田さほり; 蔀佳奈子; 眞弓佳代子; 金本佑生実; 菅谷勉; 赤坂司, 日本歯周病学会会誌(Web), 61, 126, 01 Oct. 2019
  Japanese
• ナノS‐PRGフィラー分散液と超音波スケーラー注水システムによる抗菌性歯面の構築―フィラーの歯面付着性の検討―
  眞弓佳代子; 宮治裕史; 岡本一絵; 加藤昭人; 菅谷勉, 日本歯周病学会会誌(Web), 61, 143, 01 Oct. 2019
  Japanese
• 移植再植・垂直歯根破折・剥離破折から考える歯根膜の基礎と臨床
  菅谷 勉, 特定非営利活動法人日本臨床歯周病学会年次大会プログラム・講演抄録集, 37回, 30, 30, Jun. 2019
  (NPO)日本臨床歯周病学会, Japanese
• ブラッシングが低下した歯周炎患者のプラークコントロール
  菅谷 勉, 日本歯周病学会会誌, 61, 春季特別, 102, 102, May 2019
  (NPO)日本歯周病学会, Japanese
• リン酸化プルラン含有MTAシーラーが実験的根尖性歯周炎の治癒に及ぼす効果
  河野 通俊; 大嶌 理紗; 中塚 愛; 中谷 充宣; 前田 良子; 下地 伸司; 菅谷 勉; 吉田 靖弘, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 150回, 39, 39, May 2019
  (NPO)日本歯科保存学会, Japanese
• 【1つ上を目指す歯内療法へのアプローチ 感染根管治療Retreatment IV】さまざまな感染根管治療 電磁波根尖療法(EMAT)
  富永 敏彦; 多田 瑛一朗; 高比良 一輝; 菅谷 勉, 日本歯科評論, 79, 2, 47, 52, Feb. 2019
  (株)ヒョーロン・パブリッシャーズ, Japanese
• Bone forming ability of human type-I collagen-like recombinant peptide granules and submicron β-TCP
  西田絵利香; 宮治裕史; 降籏友和; 加藤昭人; 宮田さほり; 蔀佳奈子; 眞弓佳代子; 金本佑生実; 菅谷勉; 赤坂司, 日本バイオマテリアル学会大会予稿集(Web), 41st, 2019
• リン酸三カルシウム(TCP)およびリン酸化プルラン(PPL)含有新規骨補填剤による新生骨誘導における組織学的解析
  森本康仁; 長谷川智香; 邱紫せん; 松本愛子; 桐越晶子; 森谷康人; 橋本圭司; 趙申; 前壮功仁; 本郷裕美; 菅谷勉; 吉田靖弘; 網塚憲生, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 124th, 162, 2019
  Japanese
• ナノβ‐TCP/ヒトI型コラーゲン様リコンビナントペプチド顆粒の骨形成特性評価
  西田絵利香; 宮治裕史; 降籏友和; 宮田さほり; 加藤昭人; 金本佑生実; 菅谷勉; 赤坂司, 日本再生医療学会総会(Web), 18th, ROMBUNNO.P‐02‐099 (WEB ONLY), 2019
  Japanese
• カーボンナノチューブによるチタン一生体界面のナノスケール改質と骨形成接合効果
  西田 絵利香; 宮治 裕史; 蔀 佳奈子; 眞弓 佳代子; 菅谷 勉; 梅田 純子; 近藤 勝義; 古月 文志, 日本再生歯科医学会誌, 16, 1, 49, 49, Dec. 2018
  日本再生歯科医学会, Japanese
• 酸化グラフェンスキャフォールドへの亜鉛担持による抗菌性の向上
  西田 絵利香; 宮治 裕史; 蔀 佳奈子; 菅谷 勉; 小野 博信, 日本バイオマテリアル学会大会予稿集, 40回, 274, 274, Nov. 2018
  日本バイオマテリアル学会, Japanese
• 酸化グラフェンスキャフォールドへの亜鉛担持による抗菌性の向上
  西田絵利香; 宮治裕史; 蔀佳奈子; 菅谷勉; 小野博信, 日本バイオマテリアル学会大会予稿集(Web), 40th, 274 (WEB ONLY), 274, Nov. 2018
  日本バイオマテリアル学会, Japanese
• 塩化セチルピリジニウム配合含嗽剤を用いた歯周ポケットの超音波洗浄効果におよぼす口腔清掃の影響
  鷲巣 太郎; 菅谷 勉, 日本歯周病学会会誌, 60, 秋季特別, 133, 133, Oct. 2018
  (NPO)日本歯周病学会, Japanese
• 歯周基本治療時のアロマテラピーが若年成人の自律神経活動に及ぼす影響
  竹生 寛恵; 下地 伸司; 川浪 雅光; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 149回, 175, 175, Oct. 2018
  (NPO)日本歯科保存学会, Japanese
• シンバスタチン添加α-TCP微粒子エマルション骨ペーストの特性評価および骨形成効果
  舘山 彰人; 加藤 昭人; 宮治 裕史; 西田 絵利香; 岩崎 泰彦; 藤井 秀司; 川本 康平; 蔀 佳奈子; 降籏 友和; 眞弓 佳代子; 菅谷 勉, 日本歯周病学会会誌, 60, 秋季特別, 119, 119, Oct. 2018
  (NPO)日本歯周病学会, Japanese
• シンバスタチン添加α‐TCP微粒子エマルション骨ペーストの特性評価および骨形成効果
  舘山彰人; 加藤昭人; 宮治裕史; 西田絵利香; 岩崎泰彦; 藤井秀司; 藤井秀司; 川本康平; 蔀佳奈子; 降籏友和; 眞弓佳代子; 菅谷勉, 日本歯周病学会会誌(Web), 60, 119, 01 Oct. 2018
  Japanese
• 口腔外傷による破折の最新治療 成長期〜老年期を対象
  菅谷 勉, 日本外傷歯学会総会・学術大会プログラム・抄録集, 18回, 24, 24, Jul. 2018
  (一社)日本外傷歯学会, Japanese
• 高周波電気刺激による歯根温度変化
  富永 敏彦; 多田 瑛一朗; 北池 光希; 高比良 一輝; 菅谷 勉, 日本歯内療法学会学術大会プログラム・抄録集, 39回, 105, 105, Jul. 2018
  日本歯内療法学会, Japanese
• プロービング時の出血があるSPT期の深い歯周ポケットに対する塩化セチルピリジニウム配合含嗽剤を用いた超音波洗浄の有効性
  鷲巣 太郎; 西尾 啓英; 島田 歩; 柳澤 剛; 小川 幸佑; 菅谷 勉, 日本歯周病学会会誌, 60, 春季特別, 133, 133, May 2018
  (NPO)日本歯周病学会, Japanese
• Zinc‐Fluorideガラス塗布材の細胞親和性評価
  西田絵利香; 宮治裕史; 蔀佳奈子; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 148回, 47, 47, May 2018
  (NPO)日本歯科保存学会, Japanese
• 亜鉛ガラス含有グラスアイオノマーセメントの物性および抗菌効果
  蔀佳奈子; 宮治裕史; 西田絵利香; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 148回, 48, 48, May 2018
  (NPO)日本歯科保存学会, Japanese
• 【1本の歯の保存にこだわる どの程度の損傷、状態の歯まで残すことが可能なのか?】歯根破折の行方 破折歯の抜歯基準
  二階堂 徹; 菅谷 勉, The Quintessence, 36, 12, 2585, 2591, Dec. 2017
  クインテッセンス出版(株), Japanese
• 口腔から始めるバイオマテリアル 歯周組織再生のマテリアル
  菅谷 勉, 日本バイオマテリアル学会大会予稿集, 39回, 56, 56, Nov. 2017
  日本バイオマテリアル学会, Japanese
• リゾチーム金ナノクラスター/ローズベンガル複合体の創製と光線力学的評価
  岡本一絵; 宮治裕史; 宮田さほり; 蔀佳奈子; 川崎英也; 赤坂司; 菅谷勉, 日本バイオマテリアル学会大会予稿集(Web), 39回, 132, 132, Nov. 2017
  日本バイオマテリアル学会, Japanese
• Zinc‐Fluorideガラス塗布材のイオン徐放性と抗菌効果
  西田絵利香; 宮治裕史; 蔀佳奈子; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 147回, 132, 132, Oct. 2017
  (NPO)日本歯科保存学会, Japanese
• レーザー照射による焼結水酸アパタイト基材へのフッ素担持リン酸カルシウム成膜と抗菌特性評価
  蔀佳奈子; 宮治裕史; NATHANAEL A. Joseph; 中村真紀; 大矢根綾子; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 147回, 128, 128, Oct. 2017
  (NPO)日本歯科保存学会, Japanese
• ラット皮下結合組織における試作リン酸化プルラン含有MTA根管充填用シーラーの生体適合性
  大嶌 理紗; 下地 伸司; 菅谷 勉, 日本歯内療法学会学術大会プログラム・抄録集, 38回, 130, 130, Jul. 2017
  日本歯内療法学会, Japanese
• ホルモクレゾール貼薬後の洗浄が象牙質面への接着に及ぼす効果
  鷲巣 太郎; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 146回, 132, 132, May 2017
  (NPO)日本歯科保存学会, Japanese
• 裂開状骨欠損が垂直歯根破折抵抗性に及ぼす影響
  中谷 充宣; 槌本 愛巳; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 146回, 145, 145, May 2017
  (NPO)日本歯科保存学会, Japanese
• ナノハイドロオキシアパタイト/コラーゲンの架橋剤の違いが4-META/MMA-TBBレジン上へのBMPによる骨形成に及ぼす影響
  前田 良子; 佐藤 賢人; 河野 通俊; 工藤 愛; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 146回, 160, 160, May 2017
  (NPO)日本歯科保存学会, Japanese
• 歯肉縁下細菌と血清抗体価による慢性歯周炎進行の予知判定 24ヵ月多施設前向きコホート研究
  両角 俊哉; 中川 種昭; 野口 和行; 原 宜興; 西村 英紀; 梅田 誠; 野口 俊英; 吉成 伸夫; 沼部 幸博; 伊藤 公一; 和泉 雄一; 小方 頼昌; 三邉 正人; 齋藤 淳; 佐藤 聡; 高橋 慶壮; 川浪 雅光; 花田 信弘; 高柴 正悟; 吉江 弘正; 角田 衣理加; 中村 利明; 吉村 篤利; 前田 勝正; 藤瀬 修; 上田 雅俊; 河野 智生; 福田 光男; 横井 隆; 高橋 美穂; 深谷 千絵; 関野 愉; 菅野 直之; 小林 宏明; 高井 英樹; 中山 洋平; 高野 聡美; 山田 了; 大井 麻子; 奥田 倫子; 横山 智子; 阿部 祐三; 鈴木 史彦; 菅谷 勉; 野村 義明, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 146回, 174, 174, May 2017
  (NPO)日本歯科保存学会, Japanese
• 試作リン酸化プルラン含有MTA根管充填用シーラーの根尖封鎖性
  大嶌 理紗; 下地 伸司; 菅谷 勉; 吉田 靖弘, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 146回, 40, 40, May 2017
  (NPO)日本歯科保存学会, Japanese
• 試作リン酸化プルラン含有MTA根管充填用シーラーによる根尖孔への硬組織形成
  河野 通俊; 大嶌 理紗; 熊谷 広道; 佐藤 賢人; 工藤 愛; 中谷 充宣; 前田 良子; 大歳 祐生; 下地 伸司; 菅谷 勉; 吉田 靖弘, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 146回, 139, 139, May 2017
  (NPO)日本歯科保存学会, Japanese
• 歯周治療に向けたAgクラスター/ローズベンガル複合体の特性評価
  蔀佳奈子; 宮治裕史; 宮田さほり; 菅谷勉, 日本歯周病学会会誌(Web), 59, 127, 18 Apr. 2017
  Japanese
• イオン徐放性S‐PRGフィラーの粒子サイズが抗菌性及び細胞親和性に及ぼす効果
  眞弓佳代子; 宮治裕史; 宮田さほり; 蔀佳奈子; 西田絵利香; 金本佑生実; 菅谷勉, 日本歯周病学会会誌(Web), 59, 128, 18 Apr. 2017
  Japanese
• 咬合性外傷を伴う重度慢性歯周炎患者に外傷力除去を優先して良好な治癒が得られた一症例
  加藤 昭人; 菅谷 勉, 日本歯周病学会会誌, 59, 春季特別, 152, 152, Apr. 2017
  (NPO)日本歯周病学会, Japanese
• 前歯の病的移動により生じた正中離開を基本治療と咬合調整で閉鎖した後大臼歯に再生療法を行った一症例
  齋藤 恵美子; 齋藤 彰; 加藤 熈; 菅谷 勉, 日本歯周病学会会誌, 59, 春季特別, 165, 165, Apr. 2017
  (NPO)日本歯周病学会, Japanese
• 歯周治療に向けたAgクラスター/ローズベンガル複合体の特性評価
  蔀 佳奈子; 宮治 裕史; 宮田 さほり; 菅谷 勉, 日本歯周病学会会誌, 59, 春季特別, 127, 127, Apr. 2017
  (NPO)日本歯周病学会, Japanese
• イオン徐放性S-PRGフィラーの粒子サイズが抗菌性及び細胞親和性に及ぼす効果
  眞弓 佳代子; 宮治 裕史; 宮田 さほり; 蔀 佳奈子; 西田 絵利香; 金本 佑生実; 菅谷 勉, 日本歯周病学会会誌, 59, 春季特別, 128, 128, Apr. 2017
  (NPO)日本歯周病学会, Japanese
• Bone formation by rh-BMP-2 on 4META/MMA-TBB resin containing α-TCP
  Inomata Yoshihisa; Sugaya Tsutomu; Kawanami Masamitsu, 北海道歯学雑誌, 37, 2, 124, 132, Mar. 2017, [Peer-reviewed]
  北海道歯学会, Japanese
• バイオミメティック法によるリン酸カルシウム成膜スキャフォールドの特性評価
  西田絵利香; 宮治裕史; 金本佑生実; NATHANAEL A. Joseph; 中村真紀; 大矢根綾子; 村上秀輔; 眞弓佳代子; 加藤昭人; 菅谷勉, 再生医療, 16, 390, 01 Feb. 2017
  Japanese
• バイオミメティック法によるリン酸カルシウム成膜スキャフォールドの生物学的特性
  金本佑生実; 宮治裕史; 西田絵利香; NATHANAEL A. Joseph; 中村真紀; 大矢根綾子; 村上秀輔; 眞弓佳代子; 加藤昭人; 菅谷勉, 日本バイオマテリアル学会シンポジウム予稿集, シンポジウム2016, 118, 118, Nov. 2016
  日本バイオマテリアル学会, Japanese
• Agクラスター/ローズベンガル複合体の創製と光励起による抗菌性の発現
  蔀佳奈子; 宮治裕史; 宮田さほり; 佐伯歩; 川崎英也; 長谷川裕美; 岩永敏彦; 菅谷勉, 日本バイオマテリアル学会シンポジウム予稿集, シンポジウム2016, 141, 141, Nov. 2016
  日本バイオマテリアル学会, Japanese
• ゲニピン添加マイクロ・ナノパターン化ゼラチン上での細胞接着
  蒔田玲果; 赤坂司; 宮治裕史; 宮田さほり; 菅谷勉, 日本バイオマテリアル学会シンポジウム予稿集, シンポジウム2016, 142, 142, Nov. 2016
  日本バイオマテリアル学会, Japanese
• 【接着の論点 臨床の疑問に答える】歯質保存のための接着(CQ11) 破折歯根を接着するテクニックは?
  菅谷 勉, 日本歯科評論, 増刊2016, 50, 53, Oct. 2016
  (株)ヒョーロン・パブリッシャーズ, Japanese
• ホルモクレゾールがスーパーボンド根充シーラーと象牙質の接着強さに与える影響
  鷲巣 太郎; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 145回, 126, 126, Oct. 2016
  (NPO)日本歯科保存学会, Japanese
• 歯科治療が全身状態に及ぼす影響
  下地伸司; 小田中瞳; 竹生寛恵; 大嶌理紗; 菅谷 勉; 川浪雅光, 第23回日本歯科医学会総会, 195, Oct. 2016
• イオン徐放性S‐PRGナノフィラーを用いた歯面の機能性コーティング
  眞弓佳代子; 宮治裕史; 宮田さほり; 蔀佳奈子; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 145回, 156, 156, Oct. 2016
  (NPO)日本歯科保存学会, Japanese
• レーザー援用バイオミメティック法によるレジン表面へのリン酸カルシウムの析出
  西田絵利香; 宮治裕史; NATHANAEL A.Joseph; 中村真紀; 大矢根綾子; 田中佐織; 田中佐織; 田中享; 加藤昭人; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 145回, 166, 166, Oct. 2016
  (NPO)日本歯科保存学会, Japanese
• 酸化グラフェンと近赤外線光を用いた光熱的抗菌治療法の開発
  長尾敬志; 宮治裕史; 西田絵利香; 加藤昭人; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 145回, 160, 160, Oct. 2016
  (NPO)日本歯科保存学会, Japanese
• 金クラスターを用いた抗菌的光線力学療法の開発―歯周病原菌に対する抗菌効果と細胞親和性―
  宮田さほり; 宮治裕史; 佐伯歩; 川崎英也; 田中佐織; 田中佐織; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 145回, 148, 148, Oct. 2016
  (NPO)日本歯科保存学会, Japanese
• 液中レーザー溶融法を用いて合成した銀含有リン酸カルシウム球状ナノ粒子の抗菌性評価
  宮田さほり; 宮治裕史; 中村真紀; 大矢根綾子; 菅谷勉, 日本歯周病学会会誌(Web), 58, 121, 09 Sep. 2016
  Japanese
• 酸化グラフェンスキャフォールド埋植による歯周組織治癒促進効果
  川本康平; 宮治裕史; 加藤昭人; 西田絵利香; 小川幸佑; 舘山彰人; 降旗友和; 宮田さほり; 蔀佳奈子; 菅谷勉, 日本歯周病学会会誌(Web), 58, 110, 09 Sep. 2016
  Japanese
• 日本歯周病学会監修歯周病学基礎実習動画の活用調査
  野口 和行; 原 宜興; 木下 淳博; 小林 哲夫; 菅谷 勉; 町頭 三保; 谷岡 正行; 日本歯周病学会教育委員会, 日本歯周病学会会誌, 58, 秋季特別, 120, 120, Sep. 2016
  (NPO)日本歯周病学会, Japanese
• 液中レーザー溶融法を用いて合成した銀含有リン酸カルシウム球状ナノ粒子の抗菌性評価
  宮田 さほり; 宮治 裕史; 中村 真紀; 大矢根 綾子; 菅谷 勉, 日本歯周病学会会誌, 58, 秋季特別, 121, 121, Sep. 2016
  (NPO)日本歯周病学会, Japanese
• 高周波電流刺激による骨再生
  佐藤 賢人; 菅谷 勉; 工藤 愛; 前田 良子; 富永 敏彦, 日本歯科医師会雑誌, 69, 5, 481, 481, Aug. 2016
  (公社)日本歯科医師会, Japanese
• 歯科治療が全身状態に及ぼす影響 心拍変動解析を用いた自律神経機能評価
  下地 伸司; 小田中 瞳; 竹生 寛恵; 大嶌 理紗; 菅谷 勉; 川浪 雅光, 日本歯科医師会雑誌, 69, 5, 482, 482, Aug. 2016
  (公社)日本歯科医師会, Japanese
• 高周波電流刺激の電流値と通電期間が骨再生に及ぼす影響
  佐藤 賢人; 菅谷 勉; 中谷 充宣; 前田 良子; 川浪 雅光; 富永 敏彦, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 144回, 54, 54, Jun. 2016
  (NPO)日本歯科保存学会, Japanese
• 試作リン酸化プルラン含有MTA根管充填用シーラーの封鎖性に及ぼす乾燥状態の影響
  大嶌 理紗; 下地 伸司; 菅谷 勉, 日本歯内療法学会学術大会プログラム・抄録集, 37回, 92, 92, Jun. 2016
  日本歯内療法学会, Japanese
• 試作リン酸化プルラン含有MTA根管充填用シーラーの封鎖性に及ぼす根管洗浄剤の影響
  大嶌 理紗; 菅谷 勉; 下地 伸司; 竹生 寛恵; 川浪 雅光; 吉田 靖弘, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 144回, 110, 110, Jun. 2016
  (NPO)日本歯科保存学会, Japanese
• 【補綴装置および歯の延命のための最新治療指針】(Part1)破折歯根の治療とその予防策 垂直歯根破折の実態と接着治療の理論的背景
  菅谷 勉, 歯界展望, 別冊, 補綴装置および歯の延命のための最新治療指針, 6, 12, May 2016
  医歯薬出版(株), Japanese
• 高周波電流刺激によるラット頭蓋骨欠損部への骨再生
  佐藤 賢人; 菅谷 勉; 中谷 充宣; 工藤 愛; 前田 良子; 猪俣 慶久; 川浪 雅光; 富永 敏彦, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 143回, 48, 48, Nov. 2015
  (NPO)日本歯科保存学会, Japanese
• 牛歯を用いた根管充填用シーラーMTAフィラペックスの封鎖性の評価
  大嶌 理紗; 下地 伸司; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 143回, 164, 164, Nov. 2015
  (NPO)日本歯科保存学会, Japanese
• カルシウムを混和した4-META/MMA-TBBレジン上への新生骨の接触
  柳澤 剛; 菅谷 勉; 工藤 愛; 佐藤 賢人; 猪股 慶久; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 142回, 59, 59, Jun. 2015
  (NPO)日本歯科保存学会, Japanese
• スーパーボンド根充シーラーとPMMAポイントを用いた根管充填法による破折抵抗性
  鷲巣 太郎; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 142回, 161, 161, Jun. 2015
  (NPO)日本歯科保存学会, Japanese
• 健全な若年成人における局所麻酔時の音楽鎮静の効果 心拍変動解析を用いた自律神経活動による評価
  小田中 瞳; 下地 伸司; 竹生 寛恵; 大嶌 理紗; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 142回, 53, 53, Jun. 2015
  (NPO)日本歯科保存学会, Japanese
• 局所麻酔が自律神経活動に及ぼす影響 健全な若年成人と高齢患者の比較
  下地 伸司; 小田中 瞳; 竹生 寛恵; 大嶌 理紗; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 142回, 207, 207, Jun. 2015
  (NPO)日本歯科保存学会, Japanese
• Sympathetic nervous activity during dental local anesthesia
  S Shimoji; H Odanaka; H Takefu; R Oshima; K Miyata; T Fujisawa; T Sugaya; M Kawanami, 93rd General Session & Exhibition of the IADR, Mar. 2015
• 新規開発自律神経活動モニターシステムの歯科治療への応用
  下地伸司; 小田中瞳; 竹生寛恵; 大嶌理紗; 菅谷 勉; 川浪雅光, 日本歯周病学会第4回北海道地区臨床研修会, Feb. 2015
• 表面処理材の濃度の違いによる4-META/MMA-TBBレジンと骨との接着強さの比較検討
  工藤 愛; 鷲巣 太郎; 中谷 充宣; 菅谷 勉, 接着歯学, 32, 3, 185, 185, Nov. 2014
  (一社)日本接着歯学会, Japanese
• 【臨床力アップにつながる 歯の破折の診断と処置】(第2章)歯の破折の処置(症例別対応) 歯根破折 垂直歯根破折への対応 垂直歯根破折の接着再植法
  菅谷 勉, DENTAL DIAMOND, 39, 14, 86, 91, Oct. 2014
  (株)デンタルダイヤモンド社, Japanese
• 超音波スケーラーを用いたスケーリングが高血圧症罹患高齢者の自律神経活動に及ぼす影響
  小田中 瞳; 下地 伸司; 竹生 寛恵; 大嶌 理紗; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 141回, 245, 245, Oct. 2014
  (NPO)日本歯科保存学会, Japanese
• 進行した根分岐部病変への新しい治療戦略 根分岐部デブライドメントの向上と保存的治療の適応拡大
  菅谷 勉, 日本歯周病学会会誌, 56, 秋季特別, 80, 80, Sep. 2014
  (NPO)日本歯周病学会, Japanese
• 【失活歯のマネジメント】ポストの役割と歯根破折への対応 歯髄を失ってしまった場合の考え方と術式
  菅谷 勉, 補綴臨床, 47, 4, 362, 373, Jul. 2014
  医歯薬出版(株), Japanese
• 垂直歯根破折間隙に対する根管内からの機械的切削および化学的拡大が破折間隙接着後の封鎖性に及ぼす効果
  西尾 啓英; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 140回, 28, 28, Jun. 2014
  (NPO)日本歯科保存学会, Japanese
• 表面処理剤の濃度の違いによる4-META/MMA-TBBレジンと骨との接着状態の比較検討
  工藤 愛; 菅谷 勉; 柳澤 剛; 猪股 慶久; 佐藤 賢人; 中谷 充宣; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 140回, 197, 197, Jun. 2014
  (NPO)日本歯科保存学会, Japanese
• X線CTデータを用いた破折歯の早期診断による保存治療の選択肢拡大の可能性
  齋藤 恵美子; 齋藤 彰; 渋川 義宏; 弓削 文彦; 加藤 熈; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 140回, 198, 198, Jun. 2014
  (NPO)日本歯科保存学会, Japanese
• 局所麻酔と超音波スケーラーを用いたスケーリングが健全な若年成人の自律神経活動に及ぼす影響
  小田中 瞳; 下地 伸司; 竹生 寛恵; 大嶌 理紗; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 140回, 177, 177, Jun. 2014
  (NPO)日本歯科保存学会, Japanese
• FGF2含有コラーゲンハイドロゲルスキャフォールドの根分岐部II級骨欠損への応用
  百瀬赳人; 宮治裕史; 加藤昭人; 光銭裕太; 小川幸佑; 井原朝子; 吉田崇; 村上秀輔; 西田絵利香; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 56, 春季特別, 105, 105, Apr. 2014
  (NPO)日本歯周病学会, Japanese
• 歯科保存学と連携した清掃性、長期性に優れる補綴物製作 歯周治療と歯内療法の臨床知見から補綴物形態の考え方を探る(第6回)(最終回) 根管の封鎖と歯根破折を防ぐポストのあり方(後)
  菅谷 勉; 西川 圭吾, 歯科技工, 41, 10, 1224, 1230, Oct. 2013
  医歯薬出版(株), Japanese
• 咬合性外傷単独でアタッチメントロスし固定によりゲインした症例
  菅谷 勉, 日本臨床歯周病学会会誌, 31, 1, 56, 56, Oct. 2013
  (NPO)日本臨床歯周病学会, Japanese
• カルシウムを混和した4-META/MMA-TBBレジン上への骨形成
  柳澤 剛; 菅谷 勉; 中塚 愛; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 139回, 194, 194, Oct. 2013
  (NPO)日本歯科保存学会, Japanese
• クエン酸応用が各種水酸化カルシウム製剤の除去と根管象牙質の接着性に及ぼす影響
  木村 慎吾; 菅谷 勉; 鷲巣 太郎; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 139回, 215, 215, Oct. 2013
  (NPO)日本歯科保存学会, Japanese
• 北海道大学病院における垂直歯根破折の実態調査
  中塚愛; 菅谷勉; 元木洋史; 井上加菜; 田中佐織; 宮治裕史; 川浪雅光; 坂上竜資, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 139回, 132, 132, Oct. 2013
  (NPO)日本歯科保存学会, Japanese
• 歯周ポケット内細菌検査および血漿抗体価検査によるSPT期進行の予知判定
  野村義明; 中川種昭; 両角俊哉; 菅谷 勉; 鈴木史彦; 阿部祐三; 大井麻子; 髙野聡美; 中山洋平; 小林宏明; 菅野直之; 関野 愉; 深谷千絵; 吉成伸夫; 福田光男; 河野智生; 藤瀬 修; 吉村篤利; 中村利明; 角田衣理加; 高柴正悟; 吉江弘正, 日本歯周病学会会誌, 55, 秋季特別, 124, 124, Sep. 2013
  (NPO)日本歯周病学会, Japanese, Summary national conference
• 歯科保存学と連携した清掃性、長期性に優れる補綴物製作 歯周治療と歯内療法の臨床知見から補綴物形態の考え方を探る(第5回) 根管の封鎖と歯根破折を防ぐポストのあり方(前)
  菅谷 勉; 西川 圭吾, 歯科技工, 41, 9, 1096, 1102, Sep. 2013
  医歯薬出版(株), Japanese
• 歯科保存学と連携した清掃性、長期性に優れる補綴物製作 歯周治療と歯内療法の臨床知見から補綴物形態の考え方を探る(第4回) 歯周補綴への対応
  菅谷 勉; 西川 圭吾, 歯科技工, 41, 8, 952, 961, Aug. 2013
  医歯薬出版(株), Japanese
• 歯科保存学と連携した清掃性、長期性に優れる補綴物製作 歯周治療と歯内療法の臨床知見から補綴物形態の考え方を探る(第3回) 動揺歯の固定
  菅谷 勉; 西川 圭吾, 歯科技工, 41, 7, 814, 824, Jul. 2013
  医歯薬出版(株), Japanese
• 歯科保存学と連携した清掃性、長期性に優れる補綴物製作 歯周治療と歯内療法の臨床知見から補綴物形態の考え方を探る(第2回) 根分岐部病変の治療法と歯冠形態
  菅谷 勉; 西川 圭吾, 歯科技工, 41, 5, 572, 581, May 2013
  医歯薬出版(株), Japanese
• 骨髄液内で硬化させた4-META/MMA-TBBレジン表面への硬組織形成
  洲崎 真希; 菅谷 勉; 中塚 愛; 柳澤 剛; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 138回, 63, 63, May 2013
  (NPO)日本歯科保存学会, Japanese
• レジン系シーラーの種類と根管乾燥法の違いによる根尖封鎖性への影響
  中澤 篤史; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 138回, 64, 64, May 2013
  (NPO)日本歯科保存学会, Japanese
• 垂直破折歯根の破折間隙の化学的拡大が接着後の封鎖性に及ぼす効果
  西尾 啓英; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 138回, 213, 213, May 2013
  (NPO)日本歯科保存学会, Japanese
• 垂直破折歯根の接着再植治療 垂直歯根破折の実態と接着治療の理論的背景
  菅谷 勉, 日本補綴歯科学会誌, 5, 特別号, 75, 75, May 2013
  (公社)日本補綴歯科学会, Japanese
• 【歯の長期保存の臨床 私はこうして歯を守る!】(第1章)カリエス、歯根破折、根尖性歯周炎などから歯を守る 歯根破折歯を救う
  菅谷 勉, DENTAL DIAMOND, 38, 6, 38, 45, Apr. 2013
  (株)デンタルダイヤモンド社, Japanese
• 歯科保存学と連携した清掃性、長期性に優れる補綴物製作 歯周治療と歯内療法の臨床知見から補綴物形態の考え方を探る(第1回) 補綴物の清掃性を高めるための形態のあり方
  菅谷 勉; 西川 圭吾, 歯科技工, 41, 4, 418, 425, Apr. 2013
  医歯薬出版(株), Japanese
• 【皮膚診療スキルアップ30ポイント】アトピー性皮膚炎のスキルアップ 適切な治療と患者指導に必要な最新の知見
  菅谷 誠, Derma., 203, 7, 12, Apr. 2013
  (株)全日本病院出版会, Japanese
• FGF含有コラーゲンハイドロゲルスキャフォールドの根分岐部class II骨欠損への応用
  百瀬赳人; 宮治裕史; 加藤昭人; 光銭裕太; 小川幸佑; 井原朝子; 吉田崇; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 55, 春季特別, 112, 112, Apr. 2013
  (NPO)日本歯周病学会, Japanese
• ナノβ‐TCP/コラーゲンスキャフォールドの作製と骨再生療法への応用
  井原朝子; 宮治裕史; 古月文志; 井上加菜; 光銭裕太; 金山和泉; 吉田崇; 菅谷勉; 田中佐織; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 137回, 46, 46, Oct. 2012
  (NPO)日本歯科保存学会, Japanese
• 接着を活かした垂直破折歯根の治療
  菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 136回, 10, 10, May 2012
  (NPO)日本歯科保存学会, Japanese
• 化学的表面処理後の根管象牙質とスーパーボンド根充シーラーの接着
  本間 啓史; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 136回, 128, 128, May 2012
  (NPO)日本歯科保存学会, Japanese
• クエン酸溶液による超音波根管洗浄が水酸化カルシウム除去と根管充填シーラーの接着に及ぼす影響
  鷲巣 太郎; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 136回, 142, 142, May 2012
  (NPO)日本歯科保存学会, Japanese
• 歯科用接着性レジンセメントの骨セメントへの応用
  森下 長; 中塚 愛; 洲崎 真希; 菅谷 勉; 川浪 雅光, 日本バイオマテリアル学会大会予稿集, 33回, 275, 275, Nov. 2011
  日本バイオマテリアル学会, Japanese
• ナノβ‐TCP配合コラーゲンスキャフォールドの作製と評価
  井原朝子; 宮治裕史; 古月文志; 野田坂佳伸; 大谷香織; 井上加菜; 光銭裕太; 金山和泉; 吉田崇; 田中佐織; 菅谷勉; 川浪雅光, 日本バイオマテリアル学会大会予稿集, 33回, 173, 173, Nov. 2011
  日本バイオマテリアル学会, Japanese
• ナノハイドロキシアパタイトがBMP-2による硬組織形成と象牙質吸収に及ぼす影響
  玉川 博貴; 西尾 啓英; 中澤 篤史; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 135回, 68, 68, Sep. 2011
  (NPO)日本歯科保存学会, Japanese
• 垂直破折歯根の破折間隙におけるインピーダンス値と超音波チップの歯根表面までの距離
  川端 伸也; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 135回, 188, 188, Sep. 2011
  (NPO)日本歯科保存学会, Japanese
• 新規デンタルユニット連動型自律神経活動モニターシステムの開発
  下地伸司; 菅谷勉; 根岸淳; 田中佐織; 伊藤豊; 小田島朝臣; 宮治裕史; 齋藤恵美子; 川村直人; 元木洋史; 臼井薫; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 135回, 208, 208, Sep. 2011
  (NPO)日本歯科保存学会, Japanese
• Healing of experimental apical periodontitis after apicoectomy using different sealing materials on the resected root end
  Kaori Otani; Tsutomu Sugaya; Mahito Tomita; Yukiko Hasegawa; Hirofumi Miyaji; Taichi Tenkumo; Saori Tanaka; Youji Motoki; Yasuhiro Takanawa; Masamitsu Kawanami, DENTAL MATERIALS JOURNAL, 30, 4, 485, 492, Jul. 2011
  English
• 炭酸カルシウム添加4-META/MMA-TBBレジン表面への骨形成
  逸見 優; 菅谷 勉; 中塚 愛; 中澤 篤史; 洲崎 真希; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 134回, 20, 20, May 2011
  (NPO)日本歯科保存学会, Japanese
• 北海道大学病院歯科診療センターにおける造血幹細胞移植前の口腔感染源除去―第1報 口腔ケアチームによる歯周治療について
  下地伸司; 田中佐織; 川本千春; 齊藤奈々子; 福山麻衣; 阿部貴恵; 柏崎晴彦; 山崎裕; 菅谷勉; 重松明男; 杉田純一; 西尾充史; 橋野聡; 近藤健; 田中淳司; 今村雅寛; 北川善政; 川浪雅光, 日本造血細胞移植学会総会プログラム・抄録集, 33rd, 273, 2011
  Japanese
• 臨床実習の進め方の院内勉強会を実施して 当院セラピストの意識調査結果
  菅谷 友美; 井上 克也, 理学療法いばらき, 14, 1, 17, 20, Nov. 2010
  (公社)茨城県理学療法士会, Japanese
• 根管充填法の違いが根尖孔の大きい根管における根尖封鎖性に及ぼす影響
  三上 大輔; 川村 直人; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 133回, 69, 69, Oct. 2010
  (NPO)日本歯科保存学会, Japanese
• PMMA系接着性レジンセメントに対する結合組織の反応
  森下 長; 中塚 愛; 洲崎 真希; 川村 直人; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 133回, 163, 163, Oct. 2010
  (NPO)日本歯科保存学会, Japanese
• 外傷歯学の今後の展開 接着による破折歯根の治療
  菅谷 勉, 日本外傷歯学会総会・学術大会プログラム・抄録集, 10回, 27, 27, Oct. 2010
  (一社)日本外傷歯学会, Japanese
• 北海道大学病院歯科診療センターにおける造血幹細胞移植前の歯周感染除去
  田中 佐織; 下地 伸司; 齋藤 奈々子; 川本 千春; 阿部 貴恵; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 133回, 206, 206, Oct. 2010
  (NPO)日本歯科保存学会, Japanese
• 垂直破折歯根の接着治療とその診断 術式の実際と治療経過の分析
  菅谷 勉; 大谷 香織; 川浪 雅光; 富田 真仁; 長谷川 有紀子; 加藤 熈, 歯界展望, 116, 2, 242, 249, Aug. 2010
  医歯薬出版(株), Japanese
• 垂直破折歯根の接着治療とその診断 診査結果に基づく保存・抜歯の見極めと、治療法の選択
  菅谷 勉; 田中 佐織; 元木 洋史; 川浪 雅光; 木村 喜芳; 野口 裕史; 加藤 熈, 歯界展望, 116, 1, 52, 59, Jul. 2010
  医歯薬出版(株), Japanese
• FGF2/コラーゲンハイドロゲルの骨再生スキャフォールドへの応用
  宮治裕史; 小林尚子; 菅谷勉; 井原朝子; 鷲巣太郎; 横山裕之; 光銭裕太; 川浪雅光, 北海道外科雑誌, 55, 1, 85, 85, Jun. 2010
  北海道外科学会, Japanese
• セメント質剥離破折歯の保存治療後の臨床経過
  元木 洋史; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 132回, 182, 182, May 2010
  (NPO)日本歯科保存学会, Japanese
• 超音波エンドファイルを用いた口腔内接着法による垂直破折歯根の封鎖性
  郭 穎; 中塚 愛; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 132回, 194, 194, May 2010
  (NPO)日本歯科保存学会, Japanese
• 血液の汚染時間が4-META/MMA-TBBレジンと歯根象牙質の接着に及ぼす影響
  福田 寛恵; 下地 伸司; 森川 真衣; 田中 佐織; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 132回, 65, 65, May 2010
  (NPO)日本歯科保存学会, Japanese
• THE VERIFICATION 検証の時代はじまる 垂直破折歯根のよりよい接着治療法を求めて
  菅谷 勉; 山本 俊樹; 伊部 裕子; 川村 直人, The Quintessence, 29, 4, 80, 94, Apr. 2010
  クインテッセンス出版(株), Japanese
• Periodontal Regeneration by Collagen Hydrogel-sponge Composite in 1-wall infrabony defects
  Kato Akihito; Sugaya Tsutomu; Kawanami Masamitsu; Miyaji Hirofumi; Kosen Yuta; Yokoyama Hiroyuki; Ishizuka Ryousuke; Tokunaga Keisuke; Inoue Kana; Henmi Masaru; Tanaka Saori, Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology, 2010, 0, 87, 87, 2010, [Peer-reviewed]
  JAPANESE SOCIETY OF PERIODONTOLOGY, Japanese
• Periodontal Regeneration by Collagen Hydrogel-sponge Composite into ClassII Furcation Defects
  Kosen Yuta; Kawanami Masamitsu; Miyaji Hirofumi; Kato Akihito; Yokoyama Hiroyuki; Henmi Masaru; Kobayashi Naoko; Ishizuka Ryousuke; Tanaka Saori; Sugaya Tsutomu, Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology, 2010, 0, 35, 35, 2010, [Peer-reviewed]
  JAPANESE SOCIETY OF PERIODONTOLOGY, Japanese
• Periodontal regeneration by BMP-root conditioning and collagen hydrogel-sponge composite implantation
  Kato Akihito; Kawanami Masamitsu; Miyaji Hirofumi; Ishizuka Ryousuke; Tokunaga Keisuke; Inoue Kana; Kosen Yuta; Yokoyama Hiroyuki; Tanaka Saori; Sugaya Tsutomu, Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology, 2010, 0, 60, 60, 2010, [Peer-reviewed]
  JAPANESE SOCIETY OF PERIODONTOLOGY, Japanese
• Influence of Root Dentin Surface Conditioning with Bone Morphogenetic Protein-2 on Periodontal Wound Healing in Beagle Dogs
  Hirofumi MIYAJI; Tsutomu SUGAYA; Keisuke IBE; Ryousuke ISHIZUKA; Keisuke TOKUNAGA; Masamitsu KAWANAMI, J Oral Tissue Engin, 8, 3, 173, 180, 2010
• 【エンド難症例 メカニズムと臨床対応】エンド難症例の原因分析と対応 歯科治療に起因する問題 歯根破折
  菅谷 勉, 歯界展望, 別冊, エンド難症例 メカニズムと臨床対応, 70, 73, Nov. 2009
  医歯薬出版(株), Japanese
• 4-META/MMA-TBBレジンに止血材を併用した場合の組織反応
  高輪 泰弘; 川村 直人; 中塚 愛; 本間 啓史; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 131回, 32, 32, Oct. 2009
  (NPO)日本歯科保存学会, Japanese
• 垂直破折歯根の接着治療におけるエムドゲインの歯周組織再生効果
  大谷 香織; 菅谷 勉; 富田 真仁; 長谷川 有紀子; 元木 洋史; 高輪 泰弘; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 131回, 169, 169, Oct. 2009
  (NPO)日本歯科保存学会, Japanese
• 4-META/MMA-TBBレジンと歯根象牙質の接着に及ぼす血液の影響
  福田 寛恵; 下地 伸司; 森川 真衣; 田中 佐織; 菅谷 勉; 川浪 雅光, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 131回, 174, 174, Oct. 2009
  (NPO)日本歯科保存学会, Japanese
• 根分岐部class II骨欠損にコラーゲンハイドロゲル‐スポンジ複合体を移植した場合の組織反応
  光銭裕太; 宮治裕史; 加藤昭人; 横山裕之; 逸見優; 小林尚子; 石塚良介; 田中佐織; 菅谷勉; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 131回, 244, 244, Oct. 2009
  (NPO)日本歯科保存学会, Japanese
• 培養骨髄細胞シートとBMP含有FC‐HACスポンジの併用移植による異所性骨形成
  井上加菜; 宮治裕史; 福田寛恵; 横山裕之; 田中佐織; 菅谷勉; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 131回, 62, 62, Oct. 2009
  (NPO)日本歯科保存学会, Japanese
• FGF‐2含有コラーゲンハイドロゲル‐スポンジ複合体が骨新生に与える効果
  小林尚子; 宮治裕史; 井原朝子; 鷲巣太郎; 横山裕之; 光銭裕太; 菅谷勉; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 131回, 51, 51, Oct. 2009
  (NPO)日本歯科保存学会, Japanese
• 外傷歯処置へのアプローチ 接着の観点から スーパーボンドを用いた垂直歯根破折の治療
  菅谷 勉, 日本外傷歯学会総会・学術大会プログラム・抄録集, 9回, 27, 27, Sep. 2009
  (一社)日本外傷歯学会, Japanese
• 高血圧症患者の血圧と夜間頻尿の関係
  菅谷 公男; 仲田 清剛; 下地 勉; 小田口 尚幸; 仲本 みのり; 仲里 聡; 西島 さおり; 嘉手川 豪心; 安次富 勝博; 大城 琢磨; 名嘉 栄勝, 日本排尿機能学会誌, 20, 1, 219, 219, Sep. 2009
  (一社)日本排尿機能学会, Japanese
• 骨縁下欠損への対応
  菅谷 勉, 日本臨床歯周病学会会誌, 27, 1, 78, 78, Aug. 2009
  (NPO)日本臨床歯周病学会, Japanese
• rhBMP-2に関するハイドロキシアパタイト含有量の違いによる影響の研究
  天雲 太一; 菅谷 勉; 川浪 雅光; 亘理 文夫, 北海道外科雑誌, 54, 1, 75, 76, Jun. 2009
  北海道外科学会, Japanese
• スーパーボンドC&Bを用いた垂直破折歯根の接着治療
  菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 130回, 16, 16, May 2009
  (NPO)日本歯科保存学会, Japanese
• ナノハイドロキシアパタイト-コラーゲン複合体のハイドロキシアパタイト含有量がrhBMP-2による異所性骨形成に及ぼす影響
  天雲 太一; 菅谷 勉; 川浪 雅光; 亘理 文夫, 日本歯周病学会会誌, 51, 春季特別, 131, 131, Apr. 2009
  (NPO)日本歯周病学会, Japanese
• 化学療法中に歯肉腫脹を発症した造血幹細胞移植予定患者の症例
  田中 佐織; 下地 伸司; 菅谷 勉; 川浪 雅光, 日本歯周病学会会誌, 51, 春季特別, 126, 126, Apr. 2009
  (NPO)日本歯周病学会, Japanese
• Bone Perforation and Placement of Collagen Sponge Facilitate Bone Augmentation
  Shinji Shimoji; Hirofumi Miyaji; Tsutomu Sugaya; Hifumi Tsuji; Tetsunari Hongo; Megumi Nakatsuka; Khurshid Uz Zaman; Masamitsu Kawanami, JOURNAL OF PERIODONTOLOGY, 80, 3, 505, 511, Mar. 2009
  English
• Biological effects of collagen hydrogel-sponge composite scaffold and osteoinduction by combined application with BMP after implantation in rat cranial bone defects
  Tokunaga Keisuke; Kawanami Masamitsu; Miyaji Hirofumi; Ishizuka Ryousuke; Kato Akihito; Inoue Kana; Henmi Masaru; Kosen Yuta; Tamagawa Hiroki; Sugaya Tsutomu, Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology, 2009, 0, 51, 51, 2009, [Peer-reviewed]
  JAPANESE SOCIETY OF PERIODONTOLOGY, Japanese
• エアーソルフィーのチップ形状と操作条件が根面粗さと歯石除去効率に及ぼす影響
  日歯保存誌, 52, 6, 460, 468, 2009
• セメント質剥離破折による歯周組織破壊と破折面への細菌付着
  日歯保存誌, 52, 5, 411, 418, 2009
• コラーゲンハイドロゲル-スポンジ複合体のスキャフォールドとしての有効性とBMP併用による骨増生
  得永 佳介; 菅谷 勉; 宮治 裕史; 川浪 雅光, 日本歯科保存学雑誌, 51, 6, 659, 669, Dec. 2008
  (NPO)日本歯科保存学会, Japanese
• Long-term stability of adhesion between 4-META/MMA-TBB resin and bone
  NAKATSUKA M.; KAWAMURA N.; SUGAYA T.; KAWANAMI M., Journal of the Japanese Society for Dental Materials and Devices, 27, 5, 330, 330, 25 Sep. 2008, [Peer-reviewed]
  The Japanese Society for Dental Materials and Devices (JSDMD), Japanese
• 歯科外傷への対応最前線 外傷による破折歯への対応
  菅谷 勉, 日本歯科医師会雑誌, 61, 5, 445, 445, Aug. 2008
  (公社)日本歯科医師会, Japanese
• 代用臓器・再生医学における各領域の最近の研究動向 歯周組織再生医療の最近の動向
  菅谷 勉, 北海道外科雑誌, 53, 1, 126, 126, Jun. 2008
  北海道外科学会, Japanese
• 4‐META/MMA‐TBBレジンの硬化条件と組織反応
  川村直人; 菅谷勉; 元木洋史; 宮治裕史; 川浪雅光, 北海道外科雑誌, 53, 1, 123, 123, Jun. 2008
  北海道外科学会, Japanese
• Long-term effects of root-end sealing using 4-META/MMA-TBB resin on healing of experimental apical periodontitis
  OHTANI Kaori; SUGAYA Tsutomu; TOMITA Mahito; HASEGAWA Yukiko; MOTOKI Youji; TAKANAWA Yasuhiro; KAWANAMI Masamitsu, 日本歯科保存学雑誌, 51, 春季特別, 38, 38, 07 May 2008, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• Effect of tooth polishing using Air Solfy Brush Tip
  NAKATSUKA Megumi; GUO Ying; SUGAYA Tsutomu; KAWANAMI Masamitsu; KATO Hiroshi, 日本歯科保存学雑誌, 51, 春季特別, 109, 109, 07 May 2008, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• 象牙質表面へのBMP‐2とコラーゲンハイドロゲルの塗布による歯槽骨再生と骨性癒着
  石塚良介; 宮治裕史; 加藤昭人; 得永佳介; 井上加菜; 田中陽; 菅谷勉; 川浪雅光, 再生医療, 7, 268, 22 Feb. 2008
  Japanese
• テーブルクリニック"臨床の新機軸を追究する" 効率的・効果的なフラップ手術
  菅谷 勉, 北海道歯科医師会誌, 63, 35, 37, Feb. 2008
  (一社)北海道歯科医師会, Japanese
• Long-term Healing of Experimental Apical Periodontitis after Apicoectomy Using Different Sealing Methods on Resected Root End
  OHTANI Kaori; SUGAYA Tsutomu; KAWANAMI Masamitsu, The Japanese Journal of Conservative Dentistry, 51, 6, 648, 658, 2008, [Peer-reviewed]
  This study evaluated the apical periodontal healing after root-end sealing using Super-Bond C & B®, and root-end filling using Super-EBA™ or ProRoot®MTA, when root canal infection persisted. Apical periodontitis was induced by contaminating root canals with dental plaque in mandibular premolars of 4 beagles. After 1 month, in the SB group (n=15), Super-Bond C & B® was applied to the resected surface following apicoectomy. In the EBA (n=14) and MTA (n=14) groups, a root-end cavity was prepared and filled with Super-EBA™ and ProRoot®MTA. In the control group (n=10), the root-end surface was not filled. The observations of periradicular radiolucency, the histological observation and histometrical analyses were performed at 48 weeks after surgery. The results were as follows. The areas of periradicular radiolucency and histological bone defect areas in the SB, EBA and MTA groups were significantly less than those in the control group. There were no significant differences between the SB, EBA and MTA groups. The percentage of new cementum on the apical dentin surface in the EBA and MTA groups was significantly more than that in the SB and control groups. There were no significant differences between the EBA and MTA groups. The percentage of exposed dentin that was not covered with Super-Bond C & B® or new cementum in the SB group was significantly less than that in the EBA, MTA and control groups. The EBA and MTA groups showed a significantly lower percentage than the control group. The percentage of resorbed dentin in the SB, EBA, and MTA groups was significantly less than that in the control group. There was a significant correlation between the percentage of exposed dentin and the percentage of resorbed dentin in the SB, EBA, and MTA groups. It was suggested that sealing of the resected dentin surface is important for long-term healing. It might be difficult for the new cementum to seal the resected root end surface completely following apicoectomy, and root-end sealing using Super-Bond C & B® has better potential when root canal infection remains., The Japanese Society of Conservative Dentistry, Japanese
• Effect of BMP-2 application to root dentin surface on periodontal regeneration in 1-wall infrabony defects
  KATO Akihito; MIYAJI Hirofumi; SUGAYA Tsutomu; KAWANAMI Masamitsu, Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 49, 4, 296, 304, 28 Dec. 2007, [Peer-reviewed]
  This study evaluated periodontal regeneration after the application of BMP-2 to root dentin surface in 1-wall infrabony defects.
  The mandibular first and third premolars in seven beagle dogs were extracted prior to experimental surgery. Eight weeks after the extraction, the periosteum was removed from the alveolar bone following the retraction of a partial thickness of the muco-gingival flap. Subsequently, 1-wall infrabony defects (5 mm in height, 3 mm in width) were surgically created in the mesial and distal sides of the mandibular second premolars, and in the mesial sides of the mandibular fourth premolars. Then, the periodontal ligament and the cementum on the exposed root surface were thoroughly removed. The denuded root surface was demineralized with 24% EDTA for 3 minutes. In the BMP group, BMP-2 solution (1000 μg/m) was applied to the root surface, and the flap was repositioned. BMP-2 was not applied in the control group. Histological and histometrical analyses were performed at four and eight weeks after surgery.
  At eight weeks,new bone formation, new cementum formation and ankylosis were significantly greater in the BMP group than in the control group (p<0.01). The down-growth of the junctional epithelium in the BMP group was significantly smaller than that in the control group (p<0.05).
  These findings suggest that periodontal regeneration was enhanced by the application of BMP-2 to the root dentin surface in 1-wall infrabony defects.
  Nihon Shishubyo Gakkai Kaishi (J Jpn Periodontol) 49 : 296-304, 2007., JAPANESE SOCIETY OF PERIODONTOLOGY, Japanese
• BMP-2 Application to Root Surface Enhances Periodontal Regeneration in 1-wall Infrabony Defects
  KATO Akihito; MIYAJI Hirofumi; ISHIZUKA Ryousuke; TOKUNAGA Keisuke; INOUE Kana; SUGAYA Tsutomu; KAWANAMI Masamitsu, 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 50, 秋季特別, 47, 47, 05 Oct. 2007, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• A study of periodontal therapy in hematopoietic stem cell transplant recipients
  TANAKA Saori; SUGAYA Tsutomu; MATSUMOTO Atsushi; ODAJIMA Tomoomi; MIYAJI Hirofumi; SHIMOJI Shinji; NAKAYAMA Eri; KAWANAMI Masamitsu, 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 50, 秋季特別, 207, 207, 05 Oct. 2007, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• 基礎と臨床のCROSSOVER 意図的再植法の現状と近未来
  菅谷 勉; 川浪 雅光, The Quintessence, 26, 10, 141, 148, Oct. 2007
  クインテッセンス出版(株), Japanese
• 4-META/MMA-TBBレジンと骨の接着
  菅谷 勉; 川村 直人; 中塚 愛; 川浪 雅光, 北海道外科雑誌, 52, 1, 86, 86, Jun. 2007
  北海道外科学会, Japanese
• エアーソルフィーブラシチップによる歯根陥凹部のプラーク除去効果
  中塚 愛; 菅谷 勉; 川浪 雅光; 加藤 熈, 日本歯周病学会会誌, 49, 春季特別, 180, 180, Apr. 2007
  (NPO)日本歯周病学会, Japanese
• BMP‐2処理象牙質面における硬組織形成と象牙質吸収のビスフォスフォネート投与による抑制
  本郷哲也; 宮治裕史; 川村直人; 天雲太一; 大谷香織; 得永佳介; 菅谷勉; 川浪雅光, 再生医療, 6, 279, 19 Feb. 2007
  Japanese
• Papillon-Lefevre症候群と診断した姉弟例
  角舘 直樹; 菅谷 勉; 小野 芳男; 木村 康一; 福島 千之; 藤澤 雅子; 永山 正人; 森田 学; 野村 和夫; 川浪 雅光, 日本歯周病学会会誌, 49, 3, 239, 249, 2007
• Hybrid layer seals the cementum/4-META/MMA-TBB resin interface
  Saori Tanaka; Tsutomu Sugaya; Masamitsu Kawanami; Yoshinobu Nodasaka; Toshiki Yamamoto; Hiroshi Noguchi; Yuko Tanaka; Takatsumi Ikeda; Hidehiko Sano; Sharanbir K. Sidhu, JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 80B, 1, 140, 145, Jan. 2007
  English
• Effect of BMP-2 application to root dentin surface on periodontal regeneration in 1-wall infrabony defects
  Akihito Kato; Hirofumi Miyaji; Tsutomu Sugaya; Masamitsu Kawanami, Journal of the Japanease Society of Periodontology, 49, 4, 296, 304, 2007
• A case report of siblings diagnosed with Papillon-Lefevre syndrome
  Naoki Kakudate; Tsutomu Sugaya; Yoshio Ono; Kouichi Kimura; Chiyuki Fukushima; Masako Fujisawa; Masato Nagayama; Manabu Morita; Kazuo Nomura; Masamitsu Kawanami, Journal of the Japanease Society of Periodontology, 49, 3, 239, 249, 2007
• Bonding treatment of verticl root fracture
  Jounal of the Japanease Association for Dental Science, 26, 47, 51, 2007
• Efficacy of HTML atras using movies for simulation training of periodontal surgery
  The Japanease Journal of Conservative Dentistry, 50, 1, 62, 67, 2007
• Effects of Air Solfy Brush Tips on plaque removal from maxillary molar furcation
  NAKATSUKA Megumi; SUGAYA Tsutomu; KAWANAMI Masamitsu; KATO Hiroshi, 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 49, 秋季特別, 126, 126, 11 Oct. 2006, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• 象牙質表面へのrhBMP‐2塗布によるセメント質の再生
  菅谷勉; 宮治裕史; 川浪雅光, 北海道外科雑誌, 51, 1, 79, 79, Jun. 2006
  北海道外科学会, Japanese
• Efficiency of the HTML Atlas using the Movie on Simulation Training of Periodontal Surgery
  KAWAMURA Naoto; SUGAYA Tsutomu; KAWANAMI Masamitsu, 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 49, 春季特別, 101, 101, 21 Apr. 2006, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• 【"力"と歯周病 歯周病治療における"力"の問題点とその対策】歯根破折による歯周組織破壊
  菅谷 勉, 日本歯科評論, 761, 56, 64, Mar. 2006
  (株)ヒョーロン・パブリッシャーズ, Japanese
• Bisphosphonate injection suppresses cementum like tissue formation and dentin resorption induced by BMP-2 application
  Hongo Tetsunari; Miyaji Hirofumi; Kawamura Naoto; Tenkumo Taichi; Ohtani kaori; Tokunaga Keisuke; Sugaya Tsutomu; Kawanami Masamitsu, Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology, 2006, 0, 75, 75, 2006, [Peer-reviewed]
  JAPANESE SOCIETY OF PERIODONTOLOGY, Japanese
• BMP-2 solution and collagen hydrogel application on root dentin surface promote periodontal regeneration
  Ishizuka Ryousuke; Miyaji Hirofumi; Ibe Keisuke; Kato Akihito; Tokunaga keisuke; Inoue Kana; Tanaka Akira; Sugaya Tsutomu; Kawanami Masamitsu, Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology, 2006, 0, 74, 74, 2006, [Peer-reviewed]
  JAPANESE SOCIETY OF PERIODONTOLOGY, Japanese
• Longitudinal evaluation of periodontal regeneration by BMP-2 application to root dentin surface.
  tokunaga keisuke; miyaji hirofumi; ibe keisuke; ishizuka ryousuke; sugaya tsutomu; kawanami masamitsu, Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology, 2006, 0, 42, 42, 2006, [Peer-reviewed]
  JAPANESE SOCIETY OF PERIODONTOLOGY, Japanese
• Effects of Air Solfy brush tip on plaque removal from mandibular molar furcation
  Nakatsuka Megumi; Sugaya Tsutomu; Kawanami Masamitsu; Kato Hiroshi, Program and Abstracts of Annual Meeting of the Japanese Society of Periodontology, 2006, 0, 93, 93, 2006, [Peer-reviewed]
  JAPANESE SOCIETY OF PERIODONTOLOGY, Japanese
• 根尖切除時に4-META/MMA-TBBレジンでroot-end sealingした場合の根尖性歯周炎の治癒
  日本歯科保存学雑誌, 49, 2, 282, 287, 2006
• Suppression of cementum-like tissue formation on BMP-2 applied dentin surface by bisphosphonate
  Journal of the Jananease Society J of Periodontology, 48, 4, 285, 296, 2006
• Periodontal regeneration by BMP-2 solution and collagen hydrogel application to root dentin surface
  Ryousuke Ishizuka; Hirofumi Miyaji; Tsutomu Sugaya; Masamitsu Kawanami, Journal of the Jananease Society J of Periodontology, 48, 4, 255, 266, 2006
• Healing of apical periodontitis after root-end sealing using 4-META/MMA-TBB resin following apicoectomy
  The Japanease Journal of Conservative Dentistry, 49, 2, 282, 287, 2006
• 象牙質表面処理剤の違いがBMP-2による硬組織形成と象牙質吸収に及ぼす影響
  天雲 太一; 宮治 裕史; 菅谷 勉; 川浪 雅光, 日本歯周病学会会誌, 47, 4, 269, 279, Dec. 2005
  (NPO)日本歯周病学会, Japanese
• 骨髄穿孔とコラーゲンスポンジの骨上への埋植が骨増生に及ぼす効果
  下地 伸司; 菅谷 勉; 宮治 裕史; 川浪 雅光, 日本歯科保存学雑誌, 48, 6, 926, 938, Dec. 2005
  ラットの大腿骨に骨髄穿孔を施し穿孔部の上に線維化アテロコラーゲン-熱変性アテロコラーゲン複合体スポンジを埋植した群と,骨髄穿孔はせずにスポンジ埋植のみ行った群,骨髄穿孔のみ行った群,骨膜の剥離のみ行った群を作製し,骨増生の程度を比較した.骨増生の指標として新生骨面積と新生骨の高さを計測した.処置28日後の計測において新生骨面積,新生骨の高さとも骨髄穿孔+スポンジ埋植群が他の3群に比べて有意に高値であった, (NPO)日本歯科保存学会, Japanese
• 骨髄液の移植が骨増生に及ぼす効果
  辻 ひふみ; 菅谷 勉; 宮治 裕史; 川浪 雅光, 日本歯科保存学雑誌, 48, 6, 917, 925, Dec. 2005
  線維化アテロコラーゲン-熱変性アテロコラーゲン複合体スポンジをラット大腿骨上に移植するさいスポンジに骨髄液を含浸させ,骨増生に及ぼす効果を検討した.移植後,スポンジ内部に多数の紡錘形・楕円形・立法形の細胞および血管の侵入が生じ,骨増生が促進された, (NPO)日本歯科保存学会, Japanese
• 初期歯根垂直破折への対応
  加藤 熈; 菅谷 勉; 野口 裕史; 木村 喜芳, 日本歯科評論, 757, 133, 144, Nov. 2005
  (株)ヒョーロン・パブリッシャーズ, Japanese
• Effect of decortication and onlay graft of collagen sponge on bony augmentation
  SHIMOJI Shinji; MIYAJI Hirofumi; TSUJI Hifumi; HONGO Tetsunari; NAKATSUKA Megumi; SUGAYA Tsutomu; KAWANAMI Masamitsu, 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 48, 秋季特別, 65, 65, 31 Oct. 2005, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• Effect of transplantation of collagen sponge with bone marrow on bony augmentation of rat femora
  TSUJI Hifumi; MIYAJI Hirofumi; SHIMOJI Shinji; FUKUSHIMA Chifumi; SUGAYA Tsutomu; KAWANAMI Masamitsu, 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 48, 秋季特別, 66, 66, 31 Oct. 2005, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• Epithelial down growth and width of resin in contact with periodontal tissue after bonding treatment of vertical root fracture
  MOTOKI Youji; SUGAYA Tsutomu; TOMITA Mahito; KAWAMURA Naohito; KATOU Akihito; KAWANAMI Masamitsu, 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 48, 秋季特別, 73, 73, 31 Oct. 2005, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• 垂直破折歯根の接着治療後に歯周組織に接するレジンの幅が上皮の根尖側移動に及ぼす影響
  元木 洋史; 菅谷 勉; 川浪 雅光, 日本歯科保存学雑誌, 48, 5, 733, 742, Oct. 2005
  垂直破折歯根の接着治療後に歯周組織に接する4-META/MMA-TBBレジンの幅と上皮の根尖側移動との関連性を病理組織学的に検討した.人工的に病変を作成したビーグル犬を対象に,根面の処置を行わなかったPL群と窩洞を作成しレジンを充填した群,象牙質を露出させた群を作成し,肉眼的観察と病理組織学的観察及び組織学的計測を行った.PL群は上皮根尖側移動量が僅かであり,根面に残存した歯根膜と歯肉結合組織との再付着が観察された.窩洞の幅が0.5mmの群では1.0mm,2.0mmの群よりも上皮尖側移動量が小さかった.象牙を露出させた群は窩洞を2mmとした群よりも上皮根尖移動量は小さかった.臨床的アタッチメントレベルと上皮根尖側移動量との関係は歯周組織に接するレジン幅が0.5或いは1.0mmの場合は平均で2.7〜3.0mmの差があった.つまり,4-META/MMA-TBBレジンを用いる場合,レジン面への上皮根尖移動量を小さくするためには,レジン幅をできるだけ小さくする必要があると考えられた, (NPO)日本歯科保存学会, Japanese
• "力"と歯周病 歯周病治療における"力"の問題点とその対策 "力"による歯根破折と歯周組織破壊
  菅谷 勉, 日本歯周病学会会誌, 47, 秋季特別, 51, 51, Aug. 2005
  (NPO)日本歯周病学会, Japanese
• 象牙質表面処理剤の違いがBMP‐2による硬組織形成と象牙質吸収に及ぼす影響
  天雲太一; 宮治裕史; 川村直人; 伊部敬介; 本郷哲也; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 47, 秋季特別, 78, 78, Aug. 2005
  (NPO)日本歯周病学会, Japanese
• 骨髄間質細胞とBMP含有コラーゲンスポンジの併用移植による骨新生
  宮治裕史; 本郷哲也; 天雲太一; 福島千史; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 47, 秋季特別, 101, 101, Aug. 2005
  (NPO)日本歯周病学会, Japanese
• 4-META/MMA-TBBレジンのroot-end sealing(逆根管充填)への応用
  菅谷 勉; 田中 佐織; 野口 裕史; 川村 直人; 長谷川 有紀子; 田中 裕子; 川浪 雅光; 加藤 熈, 歯界展望, 特別号, 健康な心と身体は口腔から-発ヨコハマ2004-, 225, 225, Jun. 2005
  医歯薬出版(株), Japanese
• 垂直歯根破折の診断と4META/MMA-TBBレジンを用いた接着治療
  富田 真仁; 野口 裕史; 菅谷 勉; 木村 喜芳; 川浪 雅光; 加藤 熈, 歯界展望, 特別号, 健康な心と身体は口腔から-発ヨコハマ2004-, 328, 328, Jun. 2005
  医歯薬出版(株), Japanese
• 垂直歯根破折の接着治療
  菅谷 勉; 田中 佐織; 木村 喜芳; 山本 俊樹; 富田 真仁; 長谷川 有紀子; 元木 洋史; 川浪 雅光, 日本歯科医学会誌, 24, 120, 120, Mar. 2005
  日本歯科医学会, Japanese
• エアーソルフィーの操作条件がスケーリング後の象牙質表面形状に及ぼす影響
  中塚 愛; 菅谷 勉; 川浪 雅光; 加藤 熈, 日本歯周病学会会誌, 47, 春季特別, 111, 111, Mar. 2005
  (NPO)日本歯周病学会, Japanese
• 垂直破折歯根の接着治療 予後不良の対策法 垂直破折歯根の接着治療法の成績と予後不良症例への対策法
  菅谷 勉; 加藤 熈, 歯科臨床研究, 2, 1, 32, 42, Jan. 2005
  クインテッセンス出版(株), Japanese
• BMP-2の歯根象牙質表面への塗布による歯周組織再生
  伊部 敬介; 宮治 裕史; 菅谷 勉; 川浪 雅光, 日本歯周病学会会誌, 46, 4, 278, 287, Dec. 2004
  歯周組織再生療法に応用する場合,歯根周囲には歯肉結合組織だけではなく,上皮,歯根膜,歯槽骨など多種類の組織が存在しているため,これらの組織が歯根象牙質表面への硬組織再生にどのような影響を及ぼすかは不明である.そこで,ビーグル犬の上下顎前臼歯歯根に人工的骨欠損を作製して歯根象牙質表面にbone morphogenetic protein(BMP)-2を塗布し,歯周組織再生に与える影響を病理組織学的に観察するとともに組織学的計測を行い評価した.歯根象牙質表面にBMP-2を塗布することは歯周組織の再生に有効であることが示唆された, (NPO)日本歯周病学会, Japanese
• Long-term durability of root-end sealing with 4-META/MMA-TBB resin
  Y Tanaka; T Sugaya; S Tanaka; M Kawanami, DENTAL MATERIALS JOURNAL, 23, 4, 453, 456, Dec. 2004
  English
• 1本の歯を残す治療戦略 大臼歯の垂直歯根破折に接着治療とGTR法を併用した症例
  菅谷 勉, The Quintessence, 23, 11, 130, 135, Nov. 2004
  クインテッセンス出版(株), Japanese
• Effect of root-end sealing with 4-META/MMA-TBB resin on healing of apical periodontitis
  HASEGAWA Yukiko; SUGAYA Tsutomu; TOMITA Mahito; MOTOKI Youji; KAWANAMI Masamitsu, 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 47, 秋季特別, 23, 23, 18 Oct. 2004, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• Periodontal healing of replanted teeth involving with periodontal inflammation after intra-orally bonding treatment for vertical root fracture
  TOMITA Mahito; SUGAYA Tsutomu; HASEGAWA Yukiko; MOTOKI Yoji; KAWANAMI Masamitsu, 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 47, 秋季特別, 24, 24, 18 Oct. 2004, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• Root caries prevalence in Periodontal patients
  YAMAZAKI Makiko; KATO Ayumi; KATO Tsuyoshi; SUGAYA Tsutomu; KAWANAMI Masamitsu, 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 47, 秋季特別, 114, 114, 18 Oct. 2004, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• Clinical Evaluation after the application of 4-META/MMA-TBB Resin to Furcation Perforations
  YAMAMOTO Toshiki; SUGAYA Tsutomu; KAWANAMI Masamitsu, 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 47, 秋季特別, 204, 204, 18 Oct. 2004, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• 5.象牙質表面のBMP-2処理によるセメント質再生(一般講演,第2回 日本再生歯科医学会 学術大会抄録集)
  菅谷 勉; 宮治 裕史; 川浪 雅光, 日本再生歯科医学会誌, 2, 1, 72, 72, 30 Sep. 2004, [Peer-reviewed]
  日本再生歯科医学会, Japanese
• BMP-2 application on dentin surface promotes periodontal regeneration
  IBE Keisuke; MIYAJI Hirofumi; KATO Katsuya; ISHIZUKA Ryousuke; SUGAYA Tsutomu; KAWANAMI Masamitsu, 日本歯周病学会会誌, 46, 秋季特別, 90, 90, 25 Sep. 2004, [Peer-reviewed]
  (NPO)日本歯周病学会, Japanese
• Durability of adhesion between 4-META/MMA-TBB resin and cementum.
  Yuko Tanaka; Tsutomu Sugaya; Masamitsu Kawanami, Dental materials journal, 23, 3, 265, 70, Sep. 2004, [Peer-reviewed], [Domestic magazines]
  Japanese Society for Dental Materials and Devices, English
• 4-META/MMA-TBBレジンのroot-end sealing(逆根管充填)への応用
  菅谷 勉; 田中 佐織; 野口 裕史; 川村 直人; 長谷川 有紀子; 田中 裕子; 川浪 雅光; 加藤 熈, 日本歯科医師会雑誌, 57, 4, 375, 375, Jul. 2004
  (公社)日本歯科医師会, Japanese
• 垂直歯根破折の診断と4META/MMA-TBBレジンを用いた接着治療
  富田 真仁; 野口 裕史; 菅谷 勉; 木村 喜芳; 川浪 雅光; 加藤 煕, 日本歯科医師会雑誌, 57, 4, 428, 428, Jul. 2004
  (公社)日本歯科医師会, Japanese
• 垂直破折歯根の接着治療法の病理組織学的研究と臨床成績
  菅谷 勉; 加藤 熈, 歯科臨床研究, 1, 2, 6, 15, Jul. 2004
  クインテッセンス出版(株), Japanese
• Durability of adhesion of 4-META/MMA-TBB resin to cementum
  TANAKA Yuko; SUGAYA Tsutomu; NOGUTHI Hiroshi; TANAKA Saori; KAWANAMI Masamitsu, 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 47, 春季特別, 34, 34, 07 May 2004, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• 垂直歯根破折による歯周組織破壊と治療法の基礎的研究
  菅谷 勉; 加藤 熈, 歯科臨床研究, 1, 1, 8, 17, Jan. 2004
  クインテッセンス出版(株), Japanese
• 根尖性歯周炎の治癒に対する4-META/MMA-TBBレジンによるroot-end sealingの有効性
  日本歯科保存学雑誌, 47, 622, 632, 2004
• スーパーボンドC&Bを用いたroot-end sealingの臨床
  菅谷 勉; 田中 佐織; 川村 直人; 長谷川 有紀子; 田中 裕子, 日本歯科評論, 732, 131, 140, Oct. 2003
  (株)ヒョーロン・パブリッシャーズ, Japanese
• Observation of periodontal ligament and bacteria on vertically fractured root surfaces
  TANAKA Saori; SUGAYA Tsutomu; KIMURA Kiyoshi; KAWANAMI Masamitsu, 日本歯周病学会会誌, 45, 秋季特別, 161, 161, 25 Sep. 2003, [Peer-reviewed]
  (NPO)日本歯周病学会, Japanese
• Dentin resorption and hard-tissue formation following implantation of BMP-2-applied dentin.
  H. Miyaji; T. Sugaya; K. Kato; N. Kawamura; H. Tsuji; M. Kawanami, JOURNAL OF DENTAL RESEARCH, 82, B146, B146, Jun. 2003
  English, Summary international conference
• Prognosis of periradicular surgery using 4-META/MMA-TBB resin as root-end sealant.
  T. Sugaya; H. Noguchi; H. Miyaji; M. Kawanami, JOURNAL OF DENTAL RESEARCH, 82, B117, B117, Jun. 2003
  English, Summary international conference
• Sealing ability of 4META/MMA-TBB resin as root-end sealant
  TANAKA Yuko; SUGAYA Tsutomu; NOGUCHI Hiroshi; TANAKA Saori; KAWANAMI Masamitsu, 日本歯科保存学雑誌, 46, 0, 149, 149, 06 May 2003, [Peer-reviewed]
  Japanese
• 4META/MMA-TBBレジンをroot-end sealantに用いた場合の封鎖性
  田中 裕子; 菅谷 勉; 野口 裕史; 田中 佐織; 川浪 雅光, 日本歯科保存学雑誌, 46, 春季特別, 149, 149, May 2003
  (NPO)日本歯科保存学会, Japanese
• Long-term evaluation of bonding treatment of vertical root fracture
  NOGUCHI Hiroshi; SUGAYA Tsutomu; KAWANAMI Masamitsu; IRIE Hideaki; MASAKA Nobuo, 日本歯周病学会会誌, 45, 春季特別, 138, 138, 05 Apr. 2003, [Peer-reviewed]
  (NPO)日本歯周病学会, Japanese
• 4-META/MMA-TBBレジンの硬化条件が組織反応に及ぼす影響
  日本歯科保存学雑誌, 46, 6, 853, 859, 2003
• 骨髄幹細胞のためのスキャホールドとして移植したスポンジ状コラーゲンの組成と濃度が骨欠損の治癒に及ぼす影響
  加藤 勝也; 菅谷 勉; 宮治 裕史; 川浪 雅光, 日本歯科保存学雑誌, 45, 6, 1032, 1043, Dec. 2002
  骨髄幹細胞や骨再生に働く細胞のスキャホールドとしての有効性を検討する目的で線維化アテロコラーゲンと熱変性アテロコラーゲンを用いて,組成と濃度の異なるスポンジ状コラーゲンを作成して規格化された人工的骨欠損に移植して骨髄液を浸潤させ,治癒過程に及ぼす影響を検討した.FC-HAC複合体の内部には術後1週では,オステオカルシン陰性の紡錘形の細胞や立方形の細胞が観察され,術後4週では紡錘形の細胞とオステオカルシン陽性の立方形の細胞や血管の増殖が観察され,実験期間を通して炎症性細胞浸潤が少なく,コラーゲン濃度が異なっても治癒状態に差はなかった.FCはコラーゲン濃度を上げるとコラーゲンの吸収が遅延して炎症が強く骨再生を遅延した.2.5〜5%FC-HAC複合体は骨髄幹細胞や骨再生に働く細胞のスキャホールドとして有効であり,歯周組織再生に応用できる可能性が示唆された, (NPO)日本歯科保存学会, Japanese
• Periodontal Healing after Intra-oral and Extra-oral Bonding Treatment of Experimental Vertical Root Fracture
  TOMITA Mahito; SUGAYA Tsutomu; KAWANAMI Masamitsu, Jpn J Conserv Dent, 45, 5, 787, 796, 31 Oct. 2002, [Peer-reviewed]
  歯周組織破壊の進行していない新鮮垂直破折に対して口腔内接着法と口腔外接着・再植法を行った場合の歯周組織治癒状態を比較した.ビーグル犬の歯根を垂直破折させ接着治療を行い臨床的,病理組織学的に検討した結果,垂直破折後に治療しなかった対照群は経時的にprobing depthが深くなり破折部を中心に炎症が広がり歯槽骨吸収が進行した.口腔内接着群では,対照群に類似した歯周組織破壊の生じた歯根が多く認められたが,炎症反応が殆ど認められない歯根もあり,一部の歯根では破折部位にセメント質様硬組織の形成が観察された.口腔内接着・再植群ではprobing depthは浅く,歯槽骨吸収は殆ど観察されず,一部の根面に骨性癒着が認められた.スーパーボンドC&B表面にセメント質が再生された例はなく,上皮の根尖側移動はわずかで多くは結合組織が接していた, (NPO)日本歯科保存学会, Japanese
• Influence of atelocollagen composition and the concentration in implanted sponge on healing of artificial bone defect
  KATO Katsuya; MIYAJI Hirofumi; KAWAMURA Naoto; IBE Keisuke; SUGAYA Tsutomu; KAWANAMI Masamitsu, 日本歯科保存学雑誌, 45, 秋季特別, 67, 67, 21 Oct. 2002, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• Dentin resorption and hard tissue formation on BMP-2-applied dentin surfaces
  MIYAJI Hirofumi; SUGAYA Tsutomu; KATO Katsuya; KAWAMURA Naoto; KAWANAMI Masamitsu, 日本歯科保存学雑誌, 45, 秋季特別, 117, 117, 21 Oct. 2002, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• Hard tissue formation on dentin surfaces applied with recombinant human bone morphogenetic protein-2 in the connective tissue of the palate
  H Miyaji; T Sugaya; T Miyamoto; K Kato; H Kato, JOURNAL OF PERIODONTAL RESEARCH, 37, 3, 204, 209, Jun. 2002
  English
• 4META/MMA-TBBレジンとセメント質の接着強さ
  田中 佐織; 山本 俊樹; 田中 裕子; 野口 裕史; 菅谷 勉; 川浪 雅光; 池田 考績; 佐野 英彦, 日本歯科保存学雑誌, 45, 春季特別, 54, 54, Apr. 2002
  (NPO)日本歯科保存学会, Japanese
• 実験的垂直破折歯根を接着治療した後の歯周組織反応
  富田 真仁; 菅谷 勉; 木村 喜芳; 長谷川 有紀子; 加藤 毅; 川村 直人; 川浪 雅光, 日本歯科保存学雑誌, 45, 春季特別, 70, 70, Apr. 2002
  (NPO)日本歯科保存学会, Japanese
• 歯根端切除時にスーパーボンドC&Bをroot-end sealantとして用いた場合の臨床成績
  菅谷 勉; 野口 裕史; 長谷川 有紀子; 田中 裕子; 川浪 雅光, 日本歯科保存学雑誌, 45, 春季特別, 165, 165, Apr. 2002
  (NPO)日本歯科保存学会, Japanese
• コラーゲンゲル生体接着剤を応用した歯肉剥離掻は手術後の歯周組織再生
  加藤勝也; 宮治裕史; 川村直人; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 45th, 134, 25 Mar. 2002
  Japanese
• Periodontal tissue regeneration after flap operation using collagen-gel tissue adhesive
  KATO Katsuya; MIYAJI Hirofumi; KAWAMURA Naoto; SUGAYA Tsutomu; KAWANAMI Masamitsu, Journal of the Japanese Association of Periodontology, 44, 春季特別, 134, 134, 25 Mar. 2002, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• Clinical Evaluation of Adhesive Treatment for Vertical Root Fractures
  IRIE H.; SUGAYA T.; NOGUCHI H.; MASAKA T.; KAWANAMI M.; MASAKA N., 接着歯学 = Adhesive dentistry, 19, 4, 326, 326, 15 Mar. 2002
  English
• Microleakage between 4META/MMA-TBB Resin and the Cementum in Different Etching Time
  TANAKA S.; SUGAYA T.; YAMAMOTO T.; TANAKA Y.; NOGUCHI H.; KAWANAMI M.; IKEDA T.; SANO H., 接着歯学 = Adhesive dentistry, 19, 4, 351, 351, 15 Mar. 2002
  English
• Clinical Evaluation of 4-META/MMA-TBB Resin as a Root-end Sealant Following Apicoectomy
  SUGAYA Tsutomu; NOGUCHI Hiroshi; HASEGAWA Yukiko; TANAKA Yuko; KAWANAMI Masamitsu, 日本歯科保存学雑誌, 45, 1, 62, 67, 28 Feb. 2002, [Peer-reviewed]
  Japanese
• Accessibility of an Ultrasonic Furcation Tip to Furcation Areas of Mandibular First and Second Molars
  J.Int.Acad.Periodontol, 4, 132, 137, 2002
• Effects of Debridement with an Ultrasonic Furcation Tip in Degree II Furcation Involvement of Mandibular Molars
  J.Int.Acad.Periodontol, 4, 138, 142, 2002
• HTML形式アトラスによる歯周外科手術シミュレーション教育の効率化
  奥村 健仁; 菅谷 勉; 川浪 雅光; 加藤 熈, 日本歯科保存学雑誌, 44, 6, 973, 978, Dec. 2001
  実習者が自分の実習進行状態や術中のトラブルに応じて必要な処置法や対処法をただちに参照でき,かつ教官の術中の指導を簡略化できるように,歯周外科手術アトラスをパーソナルコンピューターを用いてHyper Text Markup Language(HTML)形式で作製した.試作したHTML形式の歯周外科手術アトラスを実習に用いて,個々で実習し,実習の達成度や教官の指導時間に対してどのような効果があるかを検討した.実験群と対照群とで実習の達成度は同等であった.実習時間は実験群の方が対照群よりも長く要した.教官の指導時間は実験群の方が有意に短かった.以上の結果から,HTML形式アトラスを用いることはシミュレーション実習の効率化に有効であると考えられた, (NPO)日本歯科保存学会, Japanese
• Treatment of vertical root fracture with bonding resin cement and periodontal surgery
  SUGAYA Tsutomu; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 43, 春季特別, 115, 115, 30 Mar. 2001, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• 歯の意図的再植法の成功率向上と適応症の拡大に関する研究
  加藤 熈; 菅谷 勉; 川浪 雅光; 大森 幹朗; 野口 裕史; 奥村 健仁; 二宮 昭; 木村 喜芳; 山本 俊樹, 日本歯科医学会誌, 20, 69, 79, Mar. 2001
  歯の意図的再植法の成功率の向上と適応症の拡大を図る目的で,歯根に存在する病変に対する処置法として,垂直歯根破折に対して接着性レンジを用いた接着による保存治療,及び根尖性歯周炎に対する逆根充に接着性レンジを応用する方法を検討した.更に歯根膜が損傷している場合に歯根膜再生を促進する方法として,歯根膜細胞を培養・増殖させて移植する方法,細胞増殖作用の強い成長因子あるplatelet-derived growth factorを根面に塗布して再植する方法,及び歯周歯内病変で1〜2歯面に限局して深いポケットが根尖に及んでいる症例を捻転して再植する方法について臨床的に検討した.これらの基礎的,臨床的研究の結果は,意図的再植法の成功率の向上と適応症の拡大に極めて有用であることが認められた, 日本歯科医学会, Japanese
• 垂直歯根破折に伴う歯周組織破壊の病理組織学的研究
  木村 喜芳; 菅谷 勉; 加藤 熈, 日本歯周病学会会誌, 42, 4, 255, 266, Dec. 2000
  ビーグル犬4頭の上下顎前臼歯を分岐部で分割後,マイセルとマレットで歯根を頬舌側に垂直に破折させ,gingival index(GI),probing depth(PD),probing attachment loss(PAL)を臨床的に診査した.破折後3日,1,2,4週後に屠殺し,X線写真撮影,病理組織学的観察と計測を行った.破折後2週頃迄の初期がPDが増加しても付着の喪失は少なく,根管内の細菌が破折部に沿って歯根膜に炎症を引き起こし,2〜4週後に破折部根面に沿って付着の喪失が進み,プラークが付着し,歯周病変が合併して歯周組織破壊が急速に進行したと考えられた, (NPO)日本歯周病学会, Japanese
• Periodontal Healing after the application of 4-META/MMA-TBB Resin to Furcation Perforations
  YAMAMOTO Toshiki; SUGAYA Tsutomu; NOGUCHI Hiroshi; HORII Takeshi; KATO Hiroshi, 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 43, 秋季特別, 53, 53, 17 Oct. 2000, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• Periodontal Destruction after Vertical Root Fractures
  KIMURA Kiyoshi; NINOMIYA Akira; TOMITA Mahito; NISHIMURA Hiroshi; SUGAYA Tsutomu; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 42, 秋季特別, 127, 127, 25 Sep. 2000, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• Periodontal Destruction after Vertical Root Fractures
  KIMURA Kiyoshi; NINOMIYA Akira; TOMITA Mahito; NISHIMURA Hiroshi; SUGAYA Tsutomu; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 42, 4, 255, 266, 25 Sep. 2000
  The Japanese Society of Periodontology, Japanese
• Hard tissue formation on rhBMP-2 applied dentin surface transplanted in gingival connective tissue
  MIYAJI Hirohumi; SUGAYA Tsutomu; MIYAMOTO Tetsuro; KATO Katsuya; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 42, 秋季特別, 150, 150, 25 Sep. 2000, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• 歯周病のメインテナンス治療 メインテナンス期間中のトラブルの発現と対策法 歯根破折
  菅谷 勉; 野口 裕史; 加藤 煕, 歯界展望, 別冊, 歯周病のメインテナンス治療, 193, 199, May 2000
  医歯薬出版(株), Japanese
• 歯周病のメインテナンス治療 メインテナンス期間中のトラブルの発現と対策法 根面齲蝕
  菅谷 勉; 加藤 煕, 歯界展望, 別冊, 歯周病のメインテナンス治療, 183, 187, May 2000
  医歯薬出版(株), Japanese
• Clinical Evaluation of Periodontal Tissue after Bonding Treatment of Vertical Root Fructure
  SUGAYA T.; IWANAMI T.; MATSUMOTO A.; NOGUCHI H.; TANAKA S.; MITO M.; KATO H., Journal of the Japanese Association of Periodontology, 42, 春季特別, 99, 99, 15 Apr. 2000, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• Effect of rhBMP-2 applied to EDTA demineralized dentin on the alkaline phosphatase activity of human gingival fibroblasts
  MIYAJI Hirohumi; SUGAYA Tsutomu; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 42, 春季特別, 124, 124, 15 Apr. 2000, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• Surface Characteristic of Super-Bond Retrograde Root Filling Followed by Immediate Blood Contamination
  NOGUCHI Hiroshi; SUGAYA Tsutomu; TANAKA Saori; MIYAJI Hirohumi; KATO Hiroshi, 日本歯科保存学雑誌 = THE JAPANESE JOURNAL OF CONSERVATIVE DENTISTRY, 43, 春季特別, 97, 97, 21 Mar. 2000, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• 髄床底穿孔の処置に4-META/MMA-TBBレジンを応用した場合の歯周組織反応に関する病理組織学的検討
  日本歯科保存学会誌, 43, 1269, 1248, 2000
• rhBMP-2で処理した象牙質が歯肉線維芽細胞のアルカリ性フォスファターゼ活性と石灰化物形成に及ぼす効果
  宮治裕史; 菅谷勉; 加藤ひろし, 日本歯周病学会誌, 42, 4, 247, 254, 2000
• 歯周炎急性症状(急性歯周膿瘍)に対する2%塩酸ミノサイクリン歯科用軟膏の歯周ポケット内投与の効果について
  梅田 誠; 萩原 さつき; 魚島 マリコ; 冨永 由美子; 高森 由季子; 矢野 和子; 小島 丈尚; 野口 和行; 木村 真太郎; 本郷 興人; 岩並 知敏; 坂上 竜資; 菅谷 勉; 西岡 敏明; 根岸 淳; 松本 敦至; 伊藤 豊; 齋藤 彰; 川浪 雅光; 和田 育男; 加藤 熈; 石川 烈, 日本歯周病学会会誌, 41, 4, 436, 449, Dec. 1999
  被験者に対し,基本処置群(C群:ポケット内洗浄処置のみ)と試験薬剤群(T群:ポケット内洗浄処置+2%塩酸ミノサイクリン歯科用軟膏ポケット内注入)の2群を,盲験的な方法で比較した.術前術後において両群とも痛み等の臨床症状の改善が認められたが,T群の方がC群と比べ,より高い改善が見られた.プロービング深さや,アタッチメントレベルの変化に関してもT群の方が,高い改善が見られた.歯肉縁下プラーク細菌の総菌数は,両群とも減少したが,T群の方がより大きな減少が見られた.歯周病原性細菌の検出率に関しては,T群のみ,B.forsythus,P.gingivalis,T.denticolaの減少がみられた.T群は臨床的にも細菌学的にもC群以上に改善がみられた, (NPO)日本歯周病学会, Japanese
• Sealing ability of Super Bond retrograde root filling followed by immediate blood contamination
  NOGUCHI Hiroshi; SUGAYA Tsutomu; YAMAMOTO Toshiki; TOMITA Mahito; MATSUHASHI Chikako; KATO Hiroshi, 日本歯科保存学雑誌, 42, 秋季特別, 181, 181, 01 Oct. 1999, [Peer-reviewed]
  (NPO)日本歯科保存学会, Japanese
• Periodontal ligament cell attachment-orientation after cementum and dentin demineralization.
  K Zaman; T Sugaya; O Hongo; H Kato, JOURNAL OF DENTAL RESEARCH, 78, 5, 1122, 1122, May 1999
  English, Summary international conference
• The proliferative and alkaline phosphatase activity of Periodontal Ligament cell after application of rhPDGF-BB and rhBMP-2 to EDTA demineralized dentin
  ZAMAN Khurshid-uz; SUGAYA Tsutomu; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 41, 1, 108, 108, 30 Mar. 1999, [Peer-reviewed]
  The Japanese Society of Periodontology, English
• 歯肉細胞と歯根膜細胞の増殖及び石灰化能における象牙質の表面性状の影響
  Mahmud Muhammad Abul Hassan; 菅谷 勉; 加藤 熈, 日本歯周病学会会誌, 40, 4, 411, 420, Oct. 1998
  牛歯から象牙質ブロックを作製し,ヒト歯肉細胞(HGC),歯根膜細胞(HPLC)を播種,培養し象牙質の表面性状が歯肉細胞と歯根膜細胞の増殖及び石灰化能にどのような影響を及ぼすかを検討した.結果,象牙質の表面性状はHPLCの増殖と石灰化能,HGCの増殖に影響を及ぼすことが明らかとなった.更にHGCは低いながら石灰化能をもち,石灰化物を形成できる可能性をもつことが示唆された, (NPO)日本歯周病学会, English
• Influence of the nature of dentin surfaces on proliferation and ALP activity of gingival and periodontal ligament cells
  MAHMUD Muhammad Abul Hassan; SUGAYA Tsutomu; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 40, 3, 116, 116, 15 Sep. 1998, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• Influence of the nature of dentin surfaces on proliferation and ALP activity of gingival and periodontal ligament cells
  Hassan MAHMUD Muhammad Abul; SUGAYA Tsutomu; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 40, 4, 411, 420, 15 Sep. 1998
  The purpose of this study was to investigate the proliferation and mineralization activity of the human gingival and periodontal ligament cells in relation to the nature of the dentin surface. Three types of bovine dentin blocks were used: 1) Rp group: dentin block was root planed by hand scaler, 2) CA group: dentin block was treated by citric acid pH 1 for 3 mins and 3) Na group: citric acid treated block was further exposed to 10% NaOCl for 3 mins. Besides this, hydroxyapatite (HA) blocks were taken as 4th group. Human gingival cells (HGC) and periodontal ligament cells (HPLC) were seeded on the blocks and cultured. Alkaline phosphatase (ALP) activity and protein concentration of the attached cells on blocks were measured at the 1, 3, 5, 7, 10 and 14 days interval. Furthermore, HGC and HPLC were cultured in DMEM and the blocks were kept on the cell sheet after becoming confluent, media was changed to α-MEM supplemented with 100 nM dexamethasone and 10 mMβ-glycerophosphate. Mineralized nodules were stained weekly for 3 weeks by von-Kossa method and the area was measured in relation to the margin of blocks. The results of the protein concentration of the HGC was higher than the HPLC in the 4 groups. Among the 4 groups, protein concentration of HPLC increased at a faster rate in CA group and in HGC, protein concentration increased at a slower rate in Na group. HGC expressed very low ALP activity and was significantly lower than the HPLC. HPLC showed the highest activity in relation to CA group, and lowest activity was observed in relation to Na and HA group. Regarding the mineralized nodule; HPLC formed larger area of nodule than HGC. CA group formed the largest area of nodule in HPLC but there was no difference among the 4 groups in the HGC. The results suggest that the nature of the dentin surface could influence the cellular proliferation and mineralization activity of HPLC, and also the proliferation of HGC. As HGC are able to show mineralization activity in this study though it is very low, it can be suggested that HGC have the potentiality to form calcified tissue., The Japanese Society of Periodontology, Japanese
• Effect of Nd:YAG Laser Irradiation on the Acid-Resistance of Root Dentin Surface
  TANAKA Saori; KAWANAMI Masamitsu; SUGAYA Tsutomu; YAMAMURA Hiroko; HOSHII Chiyo; TSUKUDA Nobukazu; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 40, 3, 129, 129, 15 Sep. 1998, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• Periodontontal Regeneration by Implantation of Cultured Periodontontal Ligament Cells -Kinetic Aspects of Implanted Periodontal Ligament Cells Cultured on the Dentin Blocks in Rats-
  OKUMURA Takehito; SUGAYA Tsutomu; OHMORI Miko; MAHAMUDO Muhammad Abul Hassan; NOGUCHI Hiroshi; KATO Hiroshi; IIZUKA Tadashi; KOUGO Takao, Journal of the Japanese Association of Periodontology, 40, 3, 134, 134, 15 Sep. 1998, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• A Study of the Treatment of Vertically Fractured Root by Cohesion and Re-plantation. (II) A Histo-pathological Evaluation with Cat's Premolar.
  NOGUCHI Hiroshi; SUGAYA Tsutomu; WATANABE Nobuki; KATO Hiroshi, 日本歯科保存学雑誌, 41, 0, 37, 37, 01 May 1998, [Peer-reviewed]
  Japanese
• 垂直性骨欠損形態の診断におけるCTの有用性の検討
  岩原 弘一; 菅谷 勉; 加藤 熈, 日本歯周病学会会誌, 39, 4, 513, 519, Dec. 1997
  被験者は垂直性骨欠損を有する歯周炎患者5名とし,被験部位はフラップ手術時に撮影した口腔内写真で垂直性骨欠損形態を確認できた38歯面とした.CT法の診断精度は,頬舌側面の垂直性骨欠損では,デンタルX線写真のみ(D法)に比較して有意に高く,デンタルX線写真とポケットプロービングを併用する方法(D+P法)とは差がなかった.一方隣接面の垂直性骨欠損の有無の判定では,CT法の診断精度は,D法とD+P法とほぼ同等であったが,垂直性骨欠損の頬側壁と舌側壁の診断では,CT法の診断精度は他の2法よりも有意に高かった.以上の結果から,CT三次元立体構築像は,歯槽骨の垂直性骨欠損形態の診断に有用性が高いと思われた, (NPO)日本歯周病学会, Japanese
• Clinical Evaluation of the Periodontal Exaination System Probie III【encircled R】
  HOSHIKAWA Makoto; MITO Mitsunori; SUGAYA Tsutomu; KATO Hiroshi; MATOBA Kazunari, Journal of the Japanese Association of Periodontology, 39, 3, 92, 92, 05 Sep. 1997, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• A Study of the Treatment of Vertically Fractured Root by Cohesion and Re-plantation (I)An Evaluation with Cultured Human Periodontal Ligament Cells
  NOGUCHI Hiroshi; SUGAYA Tsutomu; MAHAMUD Abul Hassan; OKUMURA Takehito; KATO Hiroshi, 日本歯科保存学雑誌, 40, 0, 43, 43, 01 May 1997, [Peer-reviewed]
  Japanese
• A Case of Cross-arch Splint by a Professional Removable Bridge
  SUGAYA Tsutomu, Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 39, 1, 204, 205, 25 Mar. 1997, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• Clinical Evaluation of the Periodontal Eamination System CPS-1. I. Reproducibility of Probing Depth.
  MITO Mitsunori; HOSHIKAWA Makoto; SUGAYA Tsutomu; KATO Hiroshi; MATOBA Kazunari, Journal of the Japanese Association of Periodontology, 39, 1, 89, 89, 25 Mar. 1997, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• 縦破折した歯根の接着による治療法 第１報 培養歯根膜細胞を用いた接着性レジンセメントの毒性の検討
  日本歯科保存学雑誌, 40, 1445, 1452, 1997
• 縦破折した歯根の接着による治療法 第２報 接着性レジンセメントで接着・再植した場合の組織学的検討
  日本歯科保存学雑誌, 40, 1453, 1460, 1997
• 成人歯科集団検診と組み合わせて行う歯周病予防プログラムに関する研究 (第1報) CPITNを用いた予防プログラムの検討
  菅谷 勉; 向中野 浩; 渡部 亘貴, 日本歯周病学会会誌, 38, 4, 522, 528, Dec. 1996
  対象者の84.5%が歯周病に罹患しており,CPITN2以上が82.2%であり,歯周病予防処置は口腔清掃指導だけでは不十分でスケーリングが必要と思われた.又,CPITNと口腔衛生状態とは強い相関がみられたが,喫煙習慣等の因子の影響は小さく,予防処置としてプログラムに組み込む必要性は少ないと思われた.更に,アンケート調査から危険集団を選別する方法は効果が低いと考えられた, (NPO)日本歯周病学会, Japanese
• 培養歯根膜細胞移植による歯根膜,セメント質の再生に関する研究 歯根膜細胞を培養・付着させた移植象牙質片の組織学的検討
  大森 幹朗; 菅谷 勉, 日本歯周病学会会誌, 38, 4, 447, 456, Dec. 1996
  実験群は,ラット歯根膜細胞をin vitroで培養し,それをラットの象牙質片上に播種し,更に培養を行い細胞を増殖させた後,ラット頭蓋に形成した骨欠損部に移植した.一方,対照群は培養歯根膜細胞を付着させていない象牙質片を移植し,歯根移植群は新鮮な歯根膜が付着した象牙質片を移植した.実験群では2週後の6例中1例に,4週後の10例中4例に,頭蓋骨とは連続していないセメント質様硬組織の形成がみられたが,6週後には全面にアンキローシスが生じていた.対照群では2週後に,増殖した骨とアンキローシスが一部に生じており,4, 6週後には全てにアンキローシスの所見がみられた.歯根移植群では,2週後には歯根膜が残存し4週後には一部象牙質に吸収がみられ6週後には全てにアンキローシスが生じていた, (NPO)日本歯周病学会, Japanese
• 歯周病を理解する
  加藤 熈; 菅谷 勉, 歯界展望, 別冊, 歯周病を診る 検査・診断・治療のポイント, 26, 52, Nov. 1996
  医歯薬出版(株), Japanese
• ガイドラインに基づいた歯周治療の新システム
  加藤 熈; 菅谷 勉, 歯界展望, 別冊, 歯周病を診る 検査・診断・治療のポイント, 54, 61, Nov. 1996
  医歯薬出版(株), Japanese
• Periodontal Regeneration by Implantation of Cultured Periodontal Ligament Cells -Histological Evaluation of Transplanted Rat Dentin Blocks with Cultured Periodontal Ligament Cells-
  OHMORI Mikio; SUGAYA Tsutomu; MAHAMUD Abull Hassan; KAWANAMI Masamitsu; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 38, 3, 110, 110, 05 Sep. 1996, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• 歯周病患者の知覚過敏への対応
  加藤 熈; 菅谷 勉, 日本歯科評論, 642, 101, 114, Apr. 1996
  (株)ヒョーロン・パブリッシャーズ, Japanese
• Diagnosis of the Pattern of Bone Defects of Periodontally Involved Teeth -An Evaluation of Efficacy of Computed Tomography in the Diagnosis of Intrabony Defects-
  IAWAHARA Koichi; SUGAYA Tsutomu; KATO Hiroshi; KOHIRAZAWA Hideo, Journal of the Japanese Association of Periodontology, 38, 1, 119, 119, 25 Mar. 1996, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• 成人歯科集団検診と組み合わせて行う歯周病予防プログラムに関する研究 第２報 予防プログラムの実施効果の評価と林式数量化理論による分析
  渡部亘貴; 菅谷勉; 向中野浩; 加藤ひろし, 日本歯周病学会会誌, 38, 4, 529, 537, 1996
• Periodontal Regeneration by implantation of Cultured Periodontal Ligament Cells -Histological Study of Transplanted Dentin Blocks With Cultured Periodontal Ligament Cells-
  OHMORI Mikio; SUGAYA Tsutomu; KAWANAMI Masamitsu; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 37, 3, 107, 107, 06 Sep. 1995, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• Epidemiology and Prevention of periodontal disease of workers in Hokkaido : Part 2 : A follow-up study after preliminary examination
  WATANABE Nobuki; MUKAINAKANO Yutaka; SUGAYA Tsutomu; MATSUNASHI Hiroshi; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 36, 0, 118, 118, 10 Sep. 1994, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• Epidemiology and prevention of periodontal disease of workers in Hokkaido : Part 1 Results of epidemiological survey of periodontal disease
  MATSUNASHI Hiroshi; MUKAINAKANO Yutaka; SUGAYA Tsutomu; MATSUMOTO Shin-ichi; TOMOCHIKA Shoko; ENDO Takeo; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 36, 0, 139, 139, 25 Mar. 1994, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• ポケット探針型超音波スケーラーの開発と臨床応用 ポケット探針型チップの使用法と根分岐部用チップの開発
  菅谷 勉, 日本歯科医学会誌, 12, 48, 52, Mar. 1993
  ポケット探針型超音波スケーラーチップの使用条件を12通りに変化させ,根面性状に与える影響を,表面粗さ計と培養細胞を用いて検討した。その結果,適切な使用条件で用いれば,十分な根面の滑沢性と,健全歯と同レベルの付着細胞数が得られた。さらに,根分岐部には専用のチップを開発し臨床応用する目的で,下顎大臼歯分岐部に到達性の良いチップの曲率を検討してチップを試作し,in vitro,in vivoでその効果を検討した。その結果,試作した根分岐部用チップは,分岐部根面への優れた到達性と,著明な歯周炎の改善効果が認められた, 日本歯科医学会, Japanese
• A Study of Ultrasonic Furcation Scaler : Clinical Effects on Furcation Involvements of Mandibular Molars
  SUGAYA Tsutomu; KAWANAMI Masamitsu; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 34, 0, 70, 70, 16 Sep. 1992, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• 歯周組織の再生 歯槽骨からみた再生能 根分岐部病変の歯内療法による歯周組織再生について
  川浪 雅光; 菅谷 勉; 加藤 熈, 歯科ジャーナル, 36, 2, 299, 305, Aug. 1992
  (株)アークメディア, Japanese
• 深いポケットと根分岐部への超音波スケーラー(ポケット探針型および分岐部探針型チップ)の応用
  菅谷 勉, 歯界展望, 79, 5, 1167, 1167, Apr. 1992
  医歯薬出版(株), Japanese
• Effects of Ultrasonic Scaling with Probe-type Tip on Fibroblast Attachment on Periodontally Diseased Root Surfaces under Several Conditions
  SUGAYA Tsutomu; NEGISHI Jun; KAWANAMI Masamitsu; KATO Hiroshi; YUHTA Toshio, Journal of the Japanese Association of Periodontology, 33, 0, 114, 114, 20 Aug. 1991, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• Effects of Pocket Probe-type Ultrsonic Scaler on Non-surgical Periodontal Therapy
  SUGAYA Tsutomu; KAWANAMI Masamitsu; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 32, 0, 134, 134, 08 Oct. 1990, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• 高齢者における歯周治療
  加藤 ひろし; 菅谷 勉, 歯科ジャーナル, 32, 3, 353, 362, Sep. 1990
  (株)アークメディア, Japanese
• ポケット探針型超音波スケーラーの開発と臨床応用
  菅谷 勉, 日本歯科医学会誌, 9, 176, 176, Mar. 1990
  日本歯科医学会, Japanese
• 根分岐部病変の治療としてのルート・リセクション 保存的治療法について(3)
  加藤 ひろし; 菅谷 勉; 川浪 雅光, 歯界展望, 73, 3, 625, 638, Mar. 1989
  医歯薬出版(株), Japanese
• 〔歯周治療の未来を予測する 最新の歯周治療と将来〕超音波スケーラーの発展 特にチップの改良について
  加藤 ひろし; 川浪 雅光; 菅谷 勉, 歯科ジャーナル, 28, 5, 676, 684, Nov. 1988
  (株)アークメディア, Japanese
• A Study of Ultrasonic Scaler with Furcation Probe-type Tip : Accessibility of furcation area of lower first and second molar
  SUGAYA Tsutomu; TATE Takako; NAITOH Toshiya; HIRANAKA Yoshiharu; KAWANAMI Masamitsu; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 30, 0, 93, 93, 15 Oct. 1988, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• A Study of Ultrasonic Scaler with Newly Designed Probe-type Tip : Clinical efficiency in deep periodontal pockets
  IINUMA Kenji; SUGAYA Tsutomu; KATO Seiya; KUNITATE Takako; KAWANAMI Masamitsu; KATO Hiroshi, Journal of the Japanese Association of Periodontology, 29, 0, 74, 74, 10 Oct. 1987, [Peer-reviewed]
  The Japanese Society of Periodontology, Japanese
• 歯肉炎の発生に対する口呼吸の影響 : 第4報 3次元模型による粘膜の脱水乾燥について : 第29回秋季日本歯周病学会総会
  百海 渡; 渡部 とも子; 菅谷 勉; 東海林 浩二; 石川 純; 加藤 熈; 吉田 静男, 日本歯周病学会会誌, 29, 1, 268, 269, 28 Mar. 1987, [Peer-reviewed]
  特定非営利活動法人日本歯周病学会, Japanese
• ポケット探針型チップを応用した超音波スケーラーによる歯肉縁下歯垢・歯石の除去効果について(第1報) : 第29回春季日本歯周病学会総会
  加藤 誠也; 菅谷 勉; 川浪 雅光; 国立 尊子; 前沢 和宏; 石川 純, 日本歯周病学会会誌, 28, 3, 925, 925, 28 Sep. 1986, [Peer-reviewed]
  特定非営利活動法人日本歯周病学会, Japanese
• コンピュータを用いた歯周疾患患者データ管理システム : ディジタイザによるX線像のBone Level計測法 : 第28回秋季日本歯周病学会総会
  坂上 竜資; 菅谷 勉; 合志 品子; 菅野 寿一; 石川 純, 日本歯周病学会会誌, 28, 1, 410, 410, 28 Mar. 1986, [Peer-reviewed]
  特定非営利活動法人日本歯周病学会, Japanese

• 歯内療法の三種の神器
  菅谷 勉, ルートZX３高周波モジュールの特徴と臨床応用
  デンタルダイヤモンド社, Sep. 2022, [Joint work]
• 歯根破折後の判断基準・リカバリー方法
  菅谷 勉
  医療情報研究所, Aug. 2022, [Single work]
• 完全攻略 スーパーボンド
  菅谷 勉, 2章スーパーボンド基礎．No4スーパーボンドの生体親和性．P42-51．3章臨床でどう使う？スーパーボンド．No3 スーパーボンドの垂直歯根破折への適応
  クインテッセンス出版, Dec. 2018, [Joint work]
• 歯科衛生士のための最新歯周病学
  菅谷 勉, 歯周基本治療Ⅱ スケーリングとルートプレーニング、修正治療Ⅱ 歯周―歯内病変、根分岐部病変、歯周―矯正、口腔機能回復、インプラント、歯根破折の治療
  Jul. 2018, [Joint work]
• 口腔外科ハンドマニュアル
  菅谷 勉, 垂直歯根破折歯の接着治療
  クインテッセンス出版, Jul. 2018, [Joint work]
• 接着の論点
  菅谷 勉, 破折歯根を接着するテクニックは？
  ヒョーロンパブリッシャーズ, 2016, [Contributor]
• 補綴装置および歯の延命のための最新治療指針
  菅谷 勉, 1．垂直歯根破折の実態と接着治療の理論的背景
  医歯薬出版, 2016, [Contributor]
• 歯科衛生士講座 歯周病学第3版
  川浪雅光; 菅谷 勉, 第６章 歯周病の予防と治療．１歯周病の予防
  永末書店, 2016, [Contributor]
• 接着歯学
  菅谷 勉, 11章 歯周治療と接着、13章 歯の破折と接着
  医歯薬出版, 2015, [Contributor]
• 日常臨床のレベルアップ＆ヒント
  菅谷 勉, 第10章－Q２垂直歯根破折における接着再建法のコツ
  デンタルダイヤモンド社, 2015, [Contributor]
• ザ・ペリオドントロジー第2版
  菅谷 勉; 川浪雅光, 根分岐部病変
  永末書店, 2015, [Contributor]
• 臨床力アップにつながる歯の破折の診断と処置
  菅谷 勉, 5.歯根破折．4.垂直歯根破折への対応．垂直歯根破折の接着再植法
  デンタルダイヤモンド社, 2014, [Contributor]
• 垂直歯根破折歯を救え！
  菅谷 勉
  クインテッセンス出版, 2013, [Joint work]
• 道新ポケットブック「家族みんなの歯と口の健康」
  菅谷 勉; 八若保孝
  北海道新聞, 2013, [Joint editor]
• 歯の長期保存の臨床
  菅谷 勉, 第１章 カリエス、歯根破折、根尖性歯周炎などから歯を守る．歯根破折歯を救う
  デンタルダイヤモンド社, 2013, [Contributor]
• 最新歯周病学
  菅谷 勉, 第８章 垂直歯根破折による歯周病変と治療
  医歯薬出版, 2011, [Contributor]
• 口腔画像臨床診断学
  菅谷 勉, 各種症例における臨床と画像診断
  アークメディア, 2010, [Contributor]
• エンド難症例
  菅谷 勉, 歯根破折
  医歯薬出版, 2009, [Contributor]
• ザ・ペリオドントロジー
  菅谷 勉, 根分岐部病変
  永末書店, 2009, [Contributor]
• イラストレイテッド・クリニカルデンティストリー
  菅谷 勉, 咬合調整
  医歯薬出版社, 2001, [Contributor]
• 接着臨床の新たなる展開
  菅谷 勉, Root-end sealant（逆根管充填）への接着性レジンの応用
  日本歯科評論社, 2000, [Contributor]
• 歯周病のメインテナンス
  菅谷 勉, 根面齲蝕
  医歯薬出版, 2000, [Contributor]
• 歯周病を診る．検査・診断・治療のポイント
  菅谷 勉, 歯周病を理解するためにー歯周病を理解するー
  医歯薬出版, 1996, [Contributor]

• JAPAN SOCIETY FOR ADHESIVE DENTISTRY
• 日本バイオマテリアル学会
• 日本歯内療法学会
• 日本外傷歯学会
• 日本臨床歯周病学会
• 日本歯科理工学会
• 日本再生歯学会
• 日本再生医療学会
• 日本歯科保存学会
• 日本歯周病学会

• 高周波電流によるファイル未到達根管における歯髄の蒸散と根管の殺菌
  科学研究費助成事業
  01 Apr. 2021 - 31 Mar. 2024
  菅谷 勉; 西田 絵利香
  牛象牙質にΦ0.1㎜、0.4mmの模擬根管を１つまたは２つ作製し、電圧225Vで通電を行って、各根管壁の焼灼状態をSEMおよびEDSで評価した。その結果、通電時間を長くすると根管壁からCやOの検出が低下し、さらに通電時間をながくするとCaとPのみが検出されるようになって、SEMでは溶岩状に象牙質が溶融凝固した部位が増加した。この焼灼効果はΦ0.1㎜よりΦ0.4mmの方が高く、4秒の通電で根管壁はほぼ全面が溶融凝固した像を示した。Φ0.1mmと0.4mmの2根管モデルでは、それぞれ単根管モデルとほぼ同様の変化を示し、通電時間が2秒ではΦ0.1mmの根管壁に有機質が多量に残存したが、４秒に延長すると80％が蒸散可能であった。さらに、根管壁に炭化物の残存はなく、蒸散と洗浄が同時に行われている可能性が示された。
  一方、イヌの健全歯を髄腔開拡し、高周波電流を通電して歯髄の焼灼状態を病理組織学的に検討した。その結果、ファイルが根尖孔から1-2mm歯冠側で150または225Vを1秒印加することで、主根管および根尖分枝内の歯髄は壊死し、とくに血管は構造を失って研究や血漿の変性が認められた。根尖から4mm歯冠側の位置で225Vを1秒通電しても、概ね同様の結果であったが、根尖分枝内ではわずかに残髄する可能性が見られた。また、歯根膜には225Vを印加した場合にはわずかに蒸散がみられたが、その範囲は10μm程度であり、その周囲に熱変性は見られなかった。しかし、歯根膜にファイルが接した状態で通電すると、歯槽骨吸収も観察された。さらに根尖を穿通してグライドパス後に通電を行うと、歯根膜への機械的損傷による炎症が生じるとともに、根尖分枝内の焼灼がやや不十分になった。
  日本学術振興会, 基盤研究(C), 北海道大学, 21K09908
• Evaluation of the effects of osteoporosis on hard tissue formation using growth factors and ultrasound
  Grants-in-Aid for Scientific Research
  01 Apr. 2018 - 31 Mar. 2022
  YAMAJI KOZO
  This study was designed to evaluate the effects of osteoporosis on hard tissue formation using growth factor（BMP-2）and low-intensity pulsed ultrasound (LIPUS)in aged or ovariectomy (OVX) rats. The experimental sites were divided into two groups: implantation of biodegradable gelatin hydrogel (Gel) containing BMP-2(BMP group) and Gel only (Gel group).In the aged rats, the new bone formation in the BMP group was significantly increased as compared with the Gel group, and the formation was promoted by using LIPUS. In the OVX rats, the BMP group was significantly increased compared to the Gel group, but the bone mineral density was decreased compared to the non-OVX rats. On the other hand new bone formation was promoted and almost integrated with the original bone by using LIPUS.
  These results suggest that growth factor and ultrasound therapy have some effects on hard tissue formation in the case of osteoporosis.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Okayama University, 18K09575
• 高周波電流で細胞の増殖と分化を促進する革新的骨再生療法の開発
  科学研究費基盤研究
  Apr. 2017 - Mar. 2020
  菅谷 勉
  文部科学省, Principal investigator, Competitive research funding
• Effects of aging on hard tissue formation using Low-intensity pulsed ultrasound and BMP
  Grants-in-Aid for Scientific Research
  01 Apr. 2014 - 31 Mar. 2017
  YAMAJI KOZO
  This study evaluated the effects of aging on hard tissue formation induced by BMP using Low-intensity pulsed ultrasound（LIPUS）in young and aged rats.
  Tertialy dentin formation was promoted by using pulp capping material contained BMP when LIPUS treatment was performed. There was no significant difference between the young and the aged rat in the volume of tertialy dentin. Newly formed bone was promoted by implantation of material contained BMP at palatal subperiosteal site when LIPUS treatment was performed, although the volume of bone decreased with aging. These results suggest that the effects of aging on hard tissue formation might vary with the experimental sites.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Okayama University, 26462883
• TCP複合化レジンとナノHAPコラーゲンのハイブリッドによる垂直歯根破折の治療法
  科学研究費基盤研究
  2014 - 2016
  菅谷 勉
  文部科学省, Principal investigator, Competitive research funding
• 垂直歯根破折の原因と接着治療の臨床成績に関する調査研究
  8020公募研究
  2012 - 2013
  菅谷 勉
  8020財団, Principal investigator, Competitive research funding
• Development of regenerative therapy of cementum to the gap of vertical root fracture and interdigitation to the resin
  Grants-in-Aid for Scientific Research
  2011 - 2013
  SUGAYA Tsutomu; TENKUMO Taichi; NAKATSUKA Megumi
  4META/MMA-TBB resin and calcium carbonate or TCP were made composition, and elution of Ca2+ was measured. Ca2+ elution of all samples increased as the concentration becomes higher. That of beta-TCP had less than alpha-TCP and calcium carbonate. 4META/MMA-TBB resin containing alpha-TCP can facilitate direct contact between the resin and newly formed born. The hybrid layer with the bone marrow was formed on the resin surface when resin was cured in bone marrow, and the rate of bone-resin contact improved. BMP soaked collagen menbrane-nanohydroxyapatite composition was put on dentin and implanted in a femoral musle. Then a high percentage of hard tissue formation to the dentin surface was provided. 4META/MMA-TBB resin containing alpha-TCP was adhered on root surface, and BMP soaked collagen menbrane-nanohydroxyapatite composition was put on the resin. The hard tissue formation was observed on the resin.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Principal investigator, Competitive research funding, 23592789
• 垂直歯根破折の原因と接着治療の臨床成績に関する調査研究
  8020公募研究
  2012 - 2012
  菅谷 勉
  8020財団, Principal investigator, Competitive research funding
• A development of the bonding treatment of vertically fractured roots with periodontal regeneration adhesive resin using tissue engineering
  Grants-in-Aid for Scientific Research
  2010 - 2012
  TANAKA Saori; SUGAYA Tsuomu; MIYAJI Hirofumi; SHIMOJI Shinnji; YOSHIMURA Yoshitaka; TANAKA Toru; OTANI Kaori; INOUE Kana; FUKUDA Hiroe; IBARA Asako; KANAYAMA Izumi; MOMOSE Takehito; NUSHIDA Erika; MIYATA Saori
  To enhance the bonding treatment of vertically fractured roots, we aimed to the newly cementum formation on a compound of resin and dentin powder, or β-TCP which may improve the binding between resin and cementum. In vitro, cytotoxicity was assessed. It had little cytotoxicity. In vivo, we observed the newly hard tissue formation on a resin containing β-TCP applied by rhBMP-2 and cultured bone marrow stromal cell sheets. This experiment might exhibit a possibility of the newly hard tissue formation on a resin.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Coinvestigator not use grants, Competitive research funding, 22592108
• A development of the bonding treatment of vertically fractured roots with cementum regeneration on 4-META/MMA-TBB resin
  Grants-in-Aid for Scientific Research(基盤研究(C))
  2007 - 2009
  Saori TANAKA; Tsutomu SUGAYA; Hirofumi MIYAJI; Akihito KATO; Yoshitaka YOSHIMURA; Yuko IBE; Taichi TENKUMO; Kaori OTANI; Megumi NAKATSUKA; Kana INOUE; Hiroe FUKUDA; Masaru HENMI; Yuta KOSEN; Hiroki TAMAGAWA; Keishi HONMA; Hiroyuki YOKOYAMA; Taro WASHIZU; Izumi KANAYAMA; Maki SUNOSAKI; Atsushi NAKAZAWA
  To improve the bonding treatment of vertically fractured roots, we aimed to the newly cementum formation on a compound of resin and dentin powder, which may improve the binding between resin and cementum. In vitro, cytotoxicity and Micro Tensile Bond Strength (MTBS) test were assessed. It had little cytotoxicity and exhibited higher bond strength. In vivo, in some cases, we observed the newly cementum formation on a compound of resin and dentin powder. This experiment might exhibit a possibility of the newly cementum formation on a resin.
  Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(C), 北海道大学, Coinvestigator not use grants, Competitive research funding, 19592191
• ４-META/MMA-TBBレジンと骨との接着に関する研究
  ノースティック財団研究開発助成事業
  2007 - Mar. 2008
  菅谷 勉
  ノースティック財団, Principal investigator, Competitive research funding
• 垂直破折歯根の接着治療
  日本歯科医学会総合的研究推進費
  2006 - Mar. 2007
  菅谷 勉
  日本歯科医学会, Principal investigator, Competitive research funding
• Periodontal regeneration by BMP application to root surface and scaffold of periodontal ligament
  Grants-in-Aid for Scientific Research(基盤研究(B))
  2004 - 2007
  Tsutomu SUGAYA; 土門 卓文; 宮治 裕史
  The aim of the first experiment was to evaluate the effect of different dentin conditionings followed rhBMP-2 application on dentin surface. Dentin blocks were demineralized with EDTA, tetracycline-HCI or citric acid solutions for 3 minutes, and were soaked with rhBMP-2 solution. Periodontal ligament cells were seeded on each dentin blocks, and ALP activity of attachment cells were assessed. The results showed that the groups of EDTA demineralization had higher ALP activity. In the second experiment, each dentin blocks were transplanted into palatal connective tissue of rats. The histologic...
  Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Principal investigator, Competitive research funding, 16390610
• Changes in long-term adhesive property and biocompatibility of 4- META/MMA-TBB resin in vitro and vivo.
  Grants-in-Aid for Scientific Research(基盤研究(C))
  2004 - 2006
  Saori TANAKA; 菅谷 勉
  4-META/MMA-TBB resin has been used for the bonding of vertically fractured roots and apicoectomy. It has good characteristics, such as adhesive properties to dentin and cementum.However, some studies demonstrated that the collagen fibrils that are not enveloped by resin may become susceptible to water degradation in the long-term.The purpose: to evaluate the longitudinal changes in biocompatibility and mechanical properties of 4- META/MMA-TBB resin in vitro and vivo.1.Mechanical properties and Biocompatibility: After mixing the resin, these specimens each were stored in horse serum or in ai...
  Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(C), 北海道大学, Coinvestigator not use grants, Competitive research funding, 16591903
• Root-end sealing of adhesive resin
  Grants-in-Aid for Scientific Research(基盤研究(B))
  2001 - 2003
  Tsutomu SUGAYA; 土門 卓文; 池田 考績; 野口 裕史
  In the case of the presence of bacteria in the root canals, it is necessary that retrograde filling following periapical surgery prevent the leakage of bacteria from root canals into periapical tissues. The purpose of first study was to evaluate the adhesion of 4-META/MMA-TBB resin to cementum. The micro-tensile bond strength and the leakage levels obtained for the cementum were almost equal to those for the dentin, and no significant difference in durability of adhesion was recognized between dentin and cementum. The purpose of second study was to evaluate that the dye leakage from root ca...
  Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Principal investigator, Competitive research funding, 13470401
• 根面に残存する歯根膜の組織培養法を用いた新しい歯牙保存法の開発
  科学研究費補助金(萌芽的研究, 萌芽研究)
  2001 - 2002
  川浪 雅光; 齋藤 彰; 菅谷 勉
  歯周炎罹患歯の歯周組織再生において、歯根膜由来細胞は、結合組織性付着の形成に重要な役割を持っていると考えられている。この歯根膜由来細胞の性質を応用し、重度歯周炎罹患歯を歯槽部から摘出後、in vitroで組織培養し、残存歯根膜からルートプレーニングされた根面へ細胞増殖させた後に、再び口腔内に移植を行って歯と歯周組織の結合の回復をはかる治療法の開発をめざし、我々は4-6週間の歯根膜の組織培養により残存歯根膜から歯根膜由来細胞が遊走増殖し、ルートプレーニングした根面を覆うことを報告した。本年度は、残存歯根膜からルートプレーニングされた根面へ細胞増殖させた歯を移植し、移植歯とその周囲組織との間に生じる付着様式を検討した。実験には成ビーグル犬4頭の前歯25歯を用いた。被験歯を抜去後、CEJから幅4mmの根面を全周にわたり歯根膜とセメント質を除去し、ルートプレーニングを行った。根尖側の残存歯根膜はそのままにした。その後実験群は6週間組織培養を行った後、口腔内へ自家移植を行った。対照群はルートプレーニング後、直ちに自家移植を行った。観察期間は移植後4週とし、通法に従って脱灰標本を作製して病理組織学的観察を行った。歯肉上皮のdown growthは、対照群では残存歯根膜付近まで観察されたが、実験群では少なかった。歯肉上皮より根尖側のルートプレーニングされた根面において、実験群では根面に平行...
  文部科学省, 萌芽的研究, 萌芽研究, 北海道大学, Coinvestigator not use grants, Competitive research funding, 13877320
• Treatment of vertically fractured root with advanced periodontal breakdown by bonding and replantationmethod in combination with the periodontal regenerative therapy.
  Grants-in-Aid for Scientific Research(基盤研究(C))
  2000 - 2002
  Hiroshi NOGUCHI; 加藤 煕; 西岡 敏明; 伊藤 豊; 菅谷 勉; 加藤 煕
  Vertical root fractures lead to advanced periodontal breakdown with deep periodontal pockets and vertical bone defects. The purpose of this investigation was to study the treatment of vertically fractured root with advanced periodontal breakdown by bonding and replantation method in combination with the periodontal regenerative therapy.1. At first, we studied the process of periodontal destruction after vertical root fracture using 4 beagles in clinically and pathologically. Results showed that attachment loss was minimal in the early fracture stage even if probing depth was considerable, a...
  Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(C), 北海道大学, Coinvestigator not use grants, Competitive research funding, 12671845
• 歯質接着性を有する生体活性化高分子材料の開発
  ホクサイティック財団若手研究者奨励補助金
  1999 - Mar. 2000
  菅谷 勉
  ホクサイティック財団, Principal investigator, Competitive research funding
• Comprehensive study of root surface treatment and regeneration in periodontal disease
  Grants-in-Aid for Scientific Research(基盤研究(B))
  1998 - 1999
  Masaki SHIMONO; 菅谷 勉; 橋本 貞充; 高田 隆; 前田 健康; 山田 了
  The aim of this comprehensive study is development of new root surface treatment method to acquire the connective tissue attachment, and it's clinical application in which after periodontal surgery. In this project, relationship between the dynamics of periodontal ligament cells and the new root surface treatment was clarified by various methods. Followings results were obtained.(1) The mechanism of cell proliferation and differentiation in the periodontal ligament (Takata).(2) The involvement of the nerve in the regeneration process of the periodontal tissue (Maeda).(3) The mechanism of re...
  Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 東京歯科大学, Coinvestigator not use grants, Competitive research funding, 10557202
• 歯の生物学的凍結保存法の検討-超急速凍結法の影響-
  科学研究費補助金(萌芽的研究)
  1998 - 1999
  川浪 雅光; 西岡 敏明; 菅谷 勉
  1.目的:本研究では、サルの前歯部を用いて、凍結保存歯の再植後の治癒を新鮮再植歯の治癒と比較するとともに、凍結法の違いが再植後の歯周組織の治癒にどのように影響を及ぼすかについて検討した。2.方法:ニホンザル3頭の上下顎中・側切歯を被験部位とし、抜去直後に移植を行う新鮮群と超急速凍結群と緩速凍結群に無作為に振り分けた。全身麻酔および局所麻酔下で超急速凍結群と緩速凍結群の歯を、歯根膜および歯根表面を極力損傷しない様に注意しながら鉗子を用いて摘出した。培養液に摘出した歯を浸し、超急速凍結群は室温から-196℃まで瞬間的に凍結し、緩速凍結群はプログラムフリーザーによって、20度から-35度まで-0.3度c/分、-35度から-80度まで-4.5度/分、その後液体窒素にいれ、-196度まで冷却して保存した。24時間後、超急速凍結群・緩速凍結群とも室温まで急速解凍し、凍結保存歯を元の位置に再植した。また、この時新鮮群を摘出し、直ちに再植した。再植2ヶ月後に屠殺し、通法に従い、脱灰標本を作製した。標本は歯軸に対して垂直に薄切りし、HE染色を行い、光学顕微鏡にて組織学的観察を行った。3.結果:臨床検査結果では、新鮮群、超急速凍結群および緩速凍結群とも良好な治癒経過を示し、いずれの診査結果でも、3群のあいだに明らかな差は認められなかった。組織学的観察では、3群とも、大部分の歯根面は歯根膜が正常に...
  文部科学省, 萌芽的研究, 北海道大学, Coinvestigator not use grants, Competitive research funding, 10877306
• 重度歯周炎罹患歯に残存する歯根膜の組織培養による歯周組織再生に関する研究
  科学研究費補助金(萌芽的研究)
  1998 - 1999
  加藤 熈; 菅谷 勉
  我々は、歯周組織の再生に歯根膜細胞が重要な役割を果たしていることから、歯周病により保存困難と判定される歯の再生療法として、抜去歯に残存する歯根膜をin vitroで培養し歯根膜細胞を露出根面へ増殖させ被覆し、これin vivoヘ再び移植する方法に着目した。我々はすでに抜去歯に残存する歯根膜をin vitroで培養すると、歯根膜から増殖した細胞が歯根膜の喪失した根面を被覆することが可能であることを明らかにした。そこで今回は、抜去歯に残存する歯根膜を組織培養し、残存歯根膜から露出根面へ細胞が遊走増殖した状態と、アルカリフォスファターゼ(ALP)の発現部位と培養期間の関係を検討すること目的に実験を行った。成ネコ5頭の犬歯を抜去して歯冠側1/2の歯根膜をルートプレーニングして除去し、根尖側1/2の歯根膜を保存した36試験片を作製し、0、2、4、6週間培養した。その結果、培養2週では露出根面へ歯根膜由来細胞が遊走していたが、ALP発現部位は認められなかった。培養4週では細胞遊走距離が著しく増加し、ALP発現部位がわずかに認められた。培養6週では、細胞遊走距離は4週群との差はわずかで、ALP距離は平均約0.92mmと増加していたが、有意差は認められなかった。一方、4、6週群の細胞遊走距離とALP距離の相関係数は低く、統計学的に相関は認められなかった。現在、ネコの抜去歯に付着する歯根膜をi...
  文部科学省, 萌芽的研究, 北海道大学, Coinvestigator not use grants, Competitive research funding, 10877342
• Histlogical Examination of Periodontal Regeneration by Implantation of Cultured Periodontal Ligament Cell
  Grants-in-Aid for Scientific Research(基盤研究(C))
  1997 - 1998
  Tsutomu SUGAYA
  In the previous study, it was suggested that the cultured and implanted periodontal ligament cell had the regenerative potential for the periodontal ligament and cementum. But the kinetic aspects and the function of the cultured and implanted cell were not cleared. The purpose of this study is to evaluate the kinetic aspects of cultured and implanted periodontal ligament cells. In the experimental group, periodontal ligament cells were cultured and labeled on the dentin blocks in Minimum Essential Medium (MEM), supplemented with bromodeoxyuridine (BrdU) and implanted on the syngeneic rats c...
  Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(C), 北海道大学, Principal investigator, Competitive research funding, 09671942
• Development of New Bruxism Monitoring and Elucidation of Mechanism of Periodontal Destruction
  Grants-in-Aid for Scientific Research(基盤研究(B))
  1997 - 1998
  Hiroshi KATO; 本郷 興人; 根岸 淳; 菅谷 勉; 岩並 知敏; 坂上 竜資
  1. Development of a New Compact Bruxism Monitoring DeviceWe developed a new compact bruxism monitoring device, which had ease of application and auto- analyzing system. The size of the new device is 65mm x 100 mm x 35mm. It can be placed on the forehead, and 5 pick-up cables need to be connected to the electrodes with AND circuit that works only when both masseter muscles were activated. The device has 3 digital monitors that shows total bruxism time of right, left and both masseter muscles. It can objectively monitor the bruxism activities, and has less unpleasant feeling to the patients c...
  Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 09557149
• In vitroで歯根膜細胞を根面に増殖させた歯牙の自家移植に関する研究
  科学研究費補助金(萌芽的研究)
  1997 - 1998
  本郷 興人; 西岡 敏明; 岩並 知敏; 菅谷 勉
  重度歯周疾患に罹患した歯に歯周組織を再生させる新しい治療法の開発の第1段階として、抜去歯に残存する歯根膜を露出歯根面上へin vitroで培養して、後に再植する方法の開発を目指した。抜去した後に、部分的にルートプレーニングした歯根面への残存歯根膜細胞の遊走増殖を経時的に観察し検討した。実験には成ネコ7頭を用い、それぞれの犬歯を抜去して歯根側1/2の歯根膜を保存し、歯冠側1/2を露出根面とした。培養期間は、0、2、4、6週間とし、歯根膜由来細胞の観察と遊走増殖距離を計測した。細胞の遊走増殖距離は、2週では0.8mm、4週では4.6mmであった。露出歯根面と歯根膜保存根面付近で細胞密度が高く、歯冠側に向かって細胞密度が低くなる傾向があった。6週では5.2mmで、露出歯根面をほぼ全面被覆し、高い細胞密度の部分の拡大が観察された。歯根膜の残存部分では表層部分の細胞がしだいに増加し歯根膜の歯根面側の細胞は減少する傾向を示した。アルカリフォスファターゼ活性は、6週で残存歯根膜全面と歯根膜保存面と除去面の界面から2mm程度の部分までの増殖歯根膜部分に陽性反応が認められた。増殖核抗原陽性細胞は、残存歯根膜の表層と、露出した歯根表面に増殖した細胞の表層全面に認められた。これらの結果から、歯根膜が一部残存している歯根の歯根膜組織をin vitroで培養しすることによって、ルートプレーニングした歯...
  文部科学省, 萌芽的研究, 北海道大学, Coinvestigator not use grants, Competitive research funding, 09877362
• 培養歯根膜細胞移植の歯周組織再生に及ぼす効果
  科学研究費補助金(奨励研究(A))
  1996 - 1996
  菅谷 勉
  歯根膜細胞をin vitroで培養・増殖させ、これをin vivoに移植することが歯周組織再生に有効であることが示唆されている。しかし、培養した歯根膜細胞の継代数や象牙質の根面処理方法の違いが、歯周組織再生に及ぶす効果は全く不明である。近親交配ウイスター系ラットから上下前歯を採取、歯根膜細胞を培養、増殖させた。同時に抜去歯のセメント質を完全に除去し、縦2mm,横1mm,厚さ0.5mmの象牙質片を作製し、1Nクエン酸で1分間酸処理を行った。実験群は、これに培養した初代の歯根膜組織を播種し、象牙質片上でconfleuentになるまで4週間培養し移植を行った。対象群は細胞付着のない象牙質片を移植した。歯根移植群は健康な歯根膜の付着した新鮮抜去歯を獲得して直ちに移植した。3群とも移植部位はラットの頭蓋骨上とし、被験面を骨側面にして静置、Gore-Tex膜で被覆して弁を縫合した。2, 4, 6週間後に屠殺して、被験象牙質面の形態を評価した。その結果、対象群は2週ですでにほぼ前面が根吸収がみられ、4, 6週ではさらに根吸収が進行していた。歯根移植群は2, 4週は、歯根膜が残存していたが、6週では根吸収が観察された。実験群は、2週例では結合織が根面の線維と結合している状態であり、4週でははセメント質様の硬組織新生部分が認められたほか、骨性癒着や結合組織性付着の像も認められた。6週では全て骨...
  文部科学省, 奨励研究(A), 北海道大学, Principal investigator, Competitive research funding, 08771668
• treatment of fractured teeth
  The Other Research Programs
  1996
  Competitive research funding
• 培養歯根膜細胞移植による歯周組織再生の検討
  科学研究費補助金(奨励研究(A))
  1995 - 1995
  菅谷 勉
  歯根膜細胞をin vitroで培養・増殖させ、これをin vivoに移植することが歯周組織再生に有効であるかを検討した。近親交配ウイスター系ラットから上下前歯を採取、歯根膜細胞をMEM中で4週間培養し増殖させた。同時に抜去歯のセメント質を完全に除去し、縦2mm,横1mm,厚さ0.5mmの象牙質片を作製し、1Nクエン酸で1分間酸処理を行い、MEMで洗浄した。test groupは、これに培養した歯根膜細胞を播種し、象牙質片上でconfluentになるまで4週間培養し移植を行った。Negative controlは細胞付着のない象牙質片を移植した。Positive controlは健康な歯根膜の付着した新鮮抜去歯を獲得して直ちに移植した。3群とも移植部位はラットの頭蓋骨上とし、被験面を骨面側にして静置、Gore-Tex膜で被覆して弁を縫合した。各群N=2として2,4週後に屠殺し光顕観察して、被験象牙質面の形態を評価した。その結果、negative controlは2週ですでにほぼ全面が骨性癒着して根吸収がみられ、4週ではさらに根吸収が進行していた。positive controlは2,4週いずれの例も、骨性癒着して根吸収が認められた部分と、セメント質と歯根膜が残存して生理学的な形態に類似した像との両方が認められた。2週と4週との比較では、4週例の方が根呼吸の面積がやや増加している...
  文部科学省, 奨励研究(A), 北海道大学, Principal investigator, Competitive research funding, 07857136
• ポケット探針型超音波スケーラーの開発と臨床応用
  日本歯科医学会総合的研究推進費
  1992 - Mar. 1993
  菅谷 勉
  日本歯科医学会, Principal investigator, Competitive research funding
• 歯周ポケットが根尖に及んだ歯周炎罹患歯への再植法を往訪した歯周組織再生
  科学研究費補助金(奨励研究(A))
  1993 - 1993
  菅谷 勉
  歯周炎が進行して、歯周ポケットが根尖まで及ぶと保存は困難とされている。しかし、根尖に及んだポケットが歯根全周ではなく幅の狭い限局的な場合には、根面に残存する歯根膜面積は多く、これを歯周組織再生に有効に利用できる可能性が考えられる。そこで、まず実験動物に垂直性の骨欠損を作製し、患歯を一度抜歯してルートプレーニング後、Group1は元の向きに再植、Group2は180度捻転して再植、Group3は元の向きに再植してGTR、Group4は180度捻転して再植GTRを行った。現在、病理組織学的に観察を行っており、Group1に比較して2、3、4の方が歯周組織再生量は多い傾向がみられているが、部位による結果のばらつきがが多く、定量的な評価を行うには大型の動物により検討する必要が認められるため、現在その準備を進めているところである。さらに、臨床的にも同時に評価を加えており、抜歯適用のの高度歯周炎罹患歯に、患者の同意を得た上で、同様な検討を進めている。現在、症例数が少なく定量的な比較は行っていないが、Group2、3、4では著名な改善、すなわち、Probin depthの現象、Clinical attachmento levelの増加、Bone levelの増加が認められており、再植法を応用した歯周組織再生法の有効性が示唆されている。今後は臨床例を増やして行くことにより、骨欠損の形態等がど...
  文部科学省, 奨励研究(A), 北海道大学, Principal investigator, Competitive research funding, 05857213
• Periodontal Regeneration by Tissue Engineering
  1992
  Competitive research funding