Researcher basic information

• Ph.D in Science, Kobe University, Mar. 2021

• https://researchmap.jp/masayuki-k-sakata?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=202101006039439630
• https://orcid.org/0000-0003-4837-854X
• https://volley0red777.wixsite.com/sakata

• 90909904

• https://orcid.org/0000-0003-4837-854X

• 202101006039439630

• 生態学
• 陸水学
• 環境DNA

• Environmental Science/Agriculture Science, Environmental impact assessment
• Life Science, Molecular biology
• Life Science, Ecology and environment
• Environmental Science/Agriculture Science, Conservation of biological resources

• Bachelor's degree program, School of Agriculture
• Master's degree program, Graduate School of Agriculture
• Doctoral (PhD) degree program, Graduate School of Agriculture

Research activity information

• Nov. 2021, 神戸大学国際人間科学部同窓会紫陽会, 紫陽会賞
  『環境DNA分析』に関する研究
  坂田雅之
• Nov. 2021, 環境DNA学会, 優秀ポスター発表賞
  両生類を対象とした 環境 DNA メタバーコーディング検出系の開発と評価
  坂田雅之;河田萌音;倉林敦;栗田隆気;中村匡聡;白子智康;掛橋竜祐;西川 完途;モハマド=ヤジッド=ホスマン;西島太加志;樺元淳一;宮正樹;源利文
• Oct. 2018, 日本陸水学会, 優秀口頭発表賞
  堆積物由来環境DNA抽出法の改善と過去復元への展望
  坂田雅之;源利文
• Sep. 2017, 日本陸水学会, 優秀口頭発表賞
  雄物川本流における絶滅危惧種ゼニタナゴの再発見と繁殖地特定
  坂田雅之;真木伸隆;杉山秀樹;源利文

• Preservation of Filtered Environmental DNA Samples at Ambient High Temperatures
  Nao Matsumura; Qianqian Wu; Riko Matsuo; Masayuki K. Sakata; Toshifumi Minamoto
  MethodsX, 103940, 103940, Elsevier BV, May 2026, [Peer-reviewed]
  English, Scientific journal
• Landscape factors influencing the distribution of rare submerged plant species: an environmental DNA (eDNA) study
  Ayaka Fujiwara; Kei Uchida; Satoshi Yamamoto; Hirotoshi Sato; Ryohei Nakao; Masayuki K. Sakata; Norio Hayashi; Hiroki Yamanaka; Atushi Ushimaru; Toshifumi Minamoto
  PeerJ, 14, e21096, e21096, PeerJ, 21 Apr. 2026, [Peer-reviewed]
  Scientific journal, Effective conservation of rare submerged plant species requires understanding not only their habitats but also the surrounding landscape and water quality conditions. However, conventional surveys are often difficult due to the elusive nature of these plants. In this study, we applied environmental DNA (eDNA) analysis to detect species of the genus Najas , several of which are classified as endangered in Japan. We conducted field surveys and eDNA analysis across 158 ponds in western Japan and evaluated the influence of environmental and landscape variables using generalized linear models. Explanatory variables included water quality (pH, dissolved oxygen, electrical conductivity), pond size, shoreline type (soil, forest, concrete), and land use (forest, paddy field, artificial land). Our results showed that Najas presence was positively associated with lower electrical conductivity and longer forested shorelines. These findings suggest that forested pond margins may provide favorable conditions for submerged plants, while eutrophication may reduce habitat suitability. Our study demonstrates that integrating eDNA detection with landscape analysis offers a powerful and efficient approach for monitoring and conserving rare submerged plant species.
• Collection of ITS2 region reference sequences for freshwater fish in the Japanese archipelago for environmental DNA metabarcoding applications
  Gen Ito; Daisuke Sasaki; Masayuki K. Sakata; Seigo Kawase; Toshifumi Minamoto; Hiroki Yamanaka
  Metabarcoding and Metagenomics, 02 Apr. 2026, [Peer-reviewed]
  English, Scientific journal
• Stemflow for detecting mammalian environmental DNA: a case study in a zoo
  Yuya Ishihara; Kazumi Aoki; Ayumi Sakata; Masayuki K. Sakata; Kyohei Hamano; Qianqian Wu; Masaki Miya; Toshifumi Minamoto
  Metabarcoding and Metagenomics, 02 Apr. 2026, [Peer-reviewed]
  English, Scientific journal
• A Novel eDNA-Based Approach for Hybrid Detection: Implications for Conservation Management
  Masayuki K. Sakata; Nanako Yano; Akio Imamura; Hiroki Yamanaka; Toshifumi Minamoto
  bioRxiv, 27 Mar. 2026, [Lead author, Corresponding author]
  English
• Exploring the Dynamics of Environmental DNA: Effects of Early Developmental Stage and Physiological State in Chum Salmon
  Masayuki K. Sakata; Takashi Kanbe; Shunpei Sato; Hitoshi Araki
  Environmental DNA, 7, 3, e70126, May 2025, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Seven-year changes in eDNA concentrations of two dominant submerged macrophytes in Lake Shinji: Effects of salinity
  Teruhiko Takahara; Satoshi Yamagishi; Rina Shimoda; Akihiro Nagata; Masayuki K. Sakata; Hideyuki Doi; Toshifumi Minamoto
  Estuarine, Coastal and Shelf Science, 315, 109165, 109165, Elsevier BV, Apr. 2025, [Peer-reviewed]
  Scientific journal
• Reconstruction of Marimo Population Dynamics Over 200 Years Using Molecular Markers and Fossil Plankton Remains
  Jotaro Urabe; Isamu Wakana; Hajime Ohtsuki; Masayuki K. Sakata; Yurie Otake; Ryotaro Ichige; Michinobu Kuwae; Toshifumi Minamoto
  Environmental DNA, 7, 2, Wiley, 31 Mar. 2025, [Peer-reviewed]
  Scientific journal, ABSTRACT
  
  Recent efforts have focused on reconstructing the historical abundance of unfossilized organisms using environmental DNA preserved in sediments (sedDNA). This information is crucial for understanding long‐term changes in ecosystems. However, because sedDNA is prone to degradation, its quantification may not always provide accurate estimates of past abundances. To address this issue, we developed a novel method to correct for sedDNA degradation by incorporating plankton remains and applied it to estimate the historical abundance of marimo—large spherical colonies of the green alga Aegagropila brownii (formerly A. linnaei)—in Lake Akan, Japan, which is the only known habitat for large marimo. We first quantified marimo sedDNA in lake sediments dating back over 200 years. We then used our new method to estimate historical changes in their abundance from sedDNA. Analyses revealed that marimo were historically 10–100 times more abundant than they are today, but that their abundance declined in the early 20th century when influxes of muddy water and water level fluctuations occurred due to deforestation and the operation of a hydroelectric power plant. These findings align with historical eyewitness accounts, indicating that, when corrected for degradation using fossilized remains, sedDNA can be a powerful tool for reconstructing the past abundance of unfossilized organisms.
• Improved environmental DNA detection sensitivity of Opisthorchis viverrini using a multi-marker assay
  Riko Matsuo; Ayana Togetani; Poom Adisakwattana; Tippayarat Yoonuan; Orawan Phuphisut; Yanin Limpanont; Masayuki K. Sakata; Marcello Otake Sato; Megumi Sato; Toshifumi Minamoto
  Parasitology Research, 123, 12, Springer Science and Business Media LLC, 26 Dec. 2024, [Peer-reviewed]
  Scientific journal
• Human activity-associated establishment of invasive mink population estimated using environmental DNA
  Toshihiro Takaba; Masayuki K. Sakata; Takashi Kanbe; Takashi Mitsuzuka; Shouko Inoue; Hiroki Mizumoto; Takahiro Nobetsu; Hitoshi Araki
  Biological Invasions, 26, 11, 3733, 3743, Springer Science and Business Media LLC, 31 Jul. 2024, [Peer-reviewed], [Lead author]
  Scientific journal
• Detection of environmental DNA of finless porpoise (Neophocaena asiaeorientalis) in Osaka Bay, Japan
  Nagisa Hashimoto; Takashi Iwata; Natsumi Kihara; Kiyomi Nakamura; Masayuki K. Sakata; Toshifumi Minamoto
  Conservation Genetics Resources, Springer Science and Business Media LLC, 25 May 2024, [Peer-reviewed]
  English, Scientific journal, Abstract
  
  Finless porpoises (Neophocaena asiaeorientalis) currently face population decline caused by significant human activities and are categorized as endangered on the International Union for Conservation of Nature (IUCN) Red List; however, information on their habitats is currently insufficient. Although conducting visual surveys to determine the distribution of cetaceans is common, visual observation of finless porpoises is challenging owing to their specific morphological and ecological characteristics. In this study, we developed an environmental DNA (eDNA) assay for species-specific detection of finless porpoises. To test the utility of the assay, we conducted a visual survey in parallel with an eDNA survey by collecting water samples from 50 sites throughout Osaka Bay. We visually found a finless porpoise at one location and detected eDNA at nine sites, including sites near the visual observation site and those with rare sightings of finless porpoises. Therefore, in this study, we suggest that the use of eDNA analysis for distribution surveys of finless porpoises will enable more efficient surveys. The proposed eDNA technique can not only be applied to the distribution surveys finless porpoises but also to those of other cetacean species.
• Environmental detection of eumycetoma pathogens using multiplex real-time PCR for soil DNA in Sennar State, Sudan
  Hiroki Hashizume; Suguru Taga; Masayuki K. Sakata; Mahmoud Hussein; Emmanuel Edwar Siddig; Toshifumi Minamoto; Ahmed Hassan Fahal; Satoshi Kaneko
  Tropical Medicine and Health, 51, 71, Springer Science and Business Media LLC, 19 Dec. 2023, [Peer-reviewed]
  English, Scientific journal, Abstract
  
  Background
  
  Mycetoma is a chronic disease affecting the skin and subcutaneous tissue endemic in the tropical and subtropical regions. Several bacteria and fungi can cause mycetoma, but fungal mycetoma (eumycetoma) is challenging because the treatment requires a combination of a long-term antifungal agent and surgery. Although the transmission route has not yet been elucidated, infection from the soil is a leading hypothesis. However, there are few soil investigation studies, and the geographical distribution of mycetoma pathogens is not well documented. Here, we used multiplex real-time PCR technology to identify three fungal species from soil samples.
  
  Methods
  
  In total, 64 DNA samples were extracted from soil collected in seven villages in an endemic area in Sennar State, Sudan, in 2019. Primers and fluorescent probes specifically targeting the ribosomal DNA of Madurella mycetomatis, Falciformispora senegalensis, and F. tompkinsii were designed.
  
  Results
  
  Multiplex real-time PCR was performed and identified the major pathogen, M. mycetomatis that existed in most sites (95%). In addition, two other pathogens were identified from some sites. This is the first report on the use of this technique for identifying the eumycetoma causative microorganisms.
  
  Conclusions
  
  This study demonstrated that soil DNA investigation can elucidate the risk area of mycetoma-causative agents. The results will contribute to the design of prevention measures, and further large-scale studies may be effective in understanding the natural habitats of mycetoma pathogens.
• Opposite trends in environmental DNA distributions of two freshwater species under climate change
  Qianqian Wu; Jinxin Zhou; Tatsuya Komoto; Toshiyuki Ishikawa; Naoshige Goto; Masayuki K. Sakata; Daisuke Kitazawa; Toshifumi Minamoto
  Ecosphere, 14, 9, Wiley, 06 Sep. 2023, [Peer-reviewed]
  Scientific journal, Abstract
  
  Changes in the thermal structure of lake ecosystems have been documented as a response to climate change, but the dynamics of biomass distribution, which fundamentally determines species conservation, has been less studied. An interdisciplinary approach was used to demonstrate the influence of climate‐driven changes on the environmental DNA (eDNA) distribution of two species (Gymnogobius isaza and Palaemon paucidens) in Lake Biwa, the largest monomictic lake in Japan. In field surveys in 2016–2017 (full water circulation) and 2019 (partial water circulation), eDNA concentrations of these species were measured for 43 and 47 samples, respectively, collected from the lake bottom. The correlative relationship was investigated between species' eDNA concentrations and environmental variables. The species–environment relationship was then applied to species' eDNA distributions under existing and future environments calculated by a lake ecosystem model. Based on differences in the estimated eDNA distributions, we suggest that different species respond differently to climate change. The distribution of G. isaza will expand in the future if full water circulation occurs, although it appears to be independent of water circulation at present. Partial water circulation enlarges the distribution area of P. paucidens, but its eDNA concentrations will be low in the future, regardless of the extent of water circulation. These results indicate that species such as P. paucidens, which is now abundant but vulnerable to climate change, require special attention. Furthermore, our study emphasizes the potential application of interdisciplinary methodologies for improved species conservation.
• Detection of fish sedimentary DNA in aquatic systems: A review of methodological challenges and future opportunities
  Grayson P. Huston; Mark Louie D. Lopez; Yuanyu Cheng; Leighton King; Lucinda C. Duxbury; Maïlys Picard; Georgia Thomson‐Laing; Erika Myler; Caren C. Helbing; Michael T. Kinnison; Jasmine E. Saros; Irene Gregory‐Eaves; Marie‐Eve Monchamp; Susanna A. Wood; Linda Armbrecht; Gentile Francesco Ficetola; Lenka Kurte; Jordan Von Eggers; Janice Brahney; Genevieve Parent; Masayuki K. Sakata; Hideyuki Doi; Eric Capo
  Environmental DNA, 5, 6, 1449, 1472, Wiley, 28 Aug. 2023, [Peer-reviewed]
  Scientific journal, Abstract
  
  Environmental DNA studies have proliferated over the last decade, with promising data describing the diversity of organisms inhabiting aquatic and terrestrial ecosystems. The recovery of DNA present in the sediment of aquatic systems (sedDNA) has provided short‐ and long‐term data on a wide range of biological groups (e.g., photosynthetic organisms, zooplankton species) and has advanced our understanding of how environmental changes have affected aquatic communities. However, substantial challenges remain for recovering the genetic material of macro‐organisms (e.g., fish) from sediments, preventing complete reconstructions of past aquatic ecosystems, and limiting our understanding of historic, higher trophic level interactions. In this review, we outline the biotic and abiotic factors affecting the production, persistence, and transport of fish DNA from the water column to the sediments, and address questions regarding the preservation of fish DNA in sediment. We identify sources of uncertainties around the recovery of fish sedDNA arising during the sedDNA workflow. This includes methodological issues related to experimental design, DNA extraction procedures, and the selected molecular method (quantitative PCR, digital PCR, metabarcoding, metagenomics). By evaluating previous efforts (published and unpublished works) to recover fish sedDNA signals, we provide suggestions for future research and propose troubleshooting workflows for the effective detection and quantification of fish sedDNA. With further research, the use of sedDNA has the potential to be a powerful tool for inferring fish presence over time and reconstructing their population and community dynamics.
• An efficient environmental DNA detection method for rare species: a case study of a small salamander (Hynobius boulengeri)
  Masayuki K. Sakata; Daiki Takeshita; Ryohei Nishizawa; Takuya Sato; Toshifumi Minamoto
  Analytical Sciences, 39, 5, 721, 728, Springer Science and Business Media LLC, 01 Mar. 2023, [Peer-reviewed], [Lead author, Corresponding author]
  Scientific journal
• Detection and persistence of environmental DNA (eDNA) of the different developmental stages of a vector mosquito, Culex pipiens pallens
  Masayuki K. Sakata; Megumi Sato; Marcello Otake Sato; Tomoe Watanabe; Honami Mitsuishi; Tomoyuki Hikitsuchi; Jun Kobayashi; Toshifumi Minamoto
  PLOS ONE, 17, 8, e0272653, Public Library of Science (PLoS), 10 Aug. 2022, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal, Preventing mosquito-borne infectious diseases requires that vector mosquitoes are monitored and controlled. Targeting immature mosquitoes (eggs, larvae, and pupae), which have less mobility than adults, is an effective management approach. However, conducting these surveys is often difficult due to the limitations of morphological classification and survey costs. The application of environmental DNA (eDNA) analysis can solve these issues because it allows easy estimation of species distribution and morphology-independent species identification. Although a few previous studies have reported mosquito eDNA detection, there is a gap in knowledge regarding the dynamics related to the persistence of immature mosquito eDNA. We used Culex pipiens pallens, a vector of West Nile fever, as a model species. First, we developed a species-specific detection assay and confirmed its specificity using in silico and in vitro tests. Next, we conducted laboratory experiments using breeding tanks. Water samples were collected at each developmental stage. In addition, water samples were collected daily until the seventh day after emergence from the pupae. We quantified eDNA using real-time PCR with the developed assay to investigate the dynamics of mosquito eDNA. The specificity of the developed assay was confirmed by in silico and in vitro tests. Mosquito eDNA was detected at all developmental stages and detected up to seven days after emergence of pupae. In particular, high concentrations of eDNA were detected immediately after hatching from eggs and after emergence from pupae. Highly frequent positive eDNA signals were continuously detected between egg hatching and pupa hatching. Mosquito eDNA was detected immediately after the eggs were introduced, and eDNA-positive detections continued until pupae emergence, suggesting that eDNA analysis is useful for monitoring mosquito larvae. In the future, monitoring immature mosquitoes using eDNA analysis will contribute to prevent mosquito-borne infectious diseases.
• Slower growth of farmed eels stocked into rivers with higher wild eel density
  Ryoshiro Wakiya; Hikaru Itakura; Tatsumu Hirae; Tadamitsu Igari; Miyuki Manabe; Noriaki Matsuya; Katsushi Miyata; Masayuki K. Sakata; Toshifumi Minamoto; Takashi Yada; Kenzo Kaifu
  Journal of Fish Biology, Wiley, 27 Jun. 2022, [Peer-reviewed]
  Scientific journal
• Detection of multiple mycetoma pathogens using fungal metabarcoding analysis of soil DNA in an endemic area of Sudan
  Hiroki Hashizume; Suguru Taga; Masayuki K. Sakata; Mahmoud Hussein Mohamed Taha; Emmanuel Edwar Siddig; Toshifumi Minamoto; Ahmed Hassan Fahal; Satoshi Kaneko
  PLOS Neglected Tropical Diseases, 16, 3, e0010274, Public Library of Science (PLoS), 11 Mar. 2022, [Peer-reviewed]
  English, Scientific journal, Mycetoma is a tropical disease caused by several fungi and bacteria present in the soil. Fungal mycetoma and eumycetoma are especially challenging to treat; therefore, prevention, early diagnosis, and early treatment are important, but it is also necessary to understand the geographic distribution of these pathogenic fungi. In this study, we used DNA metabarcoding methodology to identify fungal species from soil samples. Soil sampling was implemented at seven villages in an endemic area of Sennar State in Sudan in 2019, and ten sampling sites were selected in each village according to land-use conditions. In total, 70 soil samples were collected from ground surfaces, and DNA in the soil was extracted with a combined method of alkaline DNA extraction and a commercial soil DNA extraction kit. The region for universal primers was selected to be the ribosomal internal transcribed spacer one region for metabarcoding. After the second PCR for DNA library preparation, the amplicon-based DNA analysis was performed using next-generation sequencing with two sets of universal primers. A total of twelve mycetoma-causative fungal species were identified, including the prime agent, Madurella mycetomatis, and additional pathogens, Falciformispora senegalensis and Falciformispora tompkinsii, in 53 soil samples. This study demonstrated that soil DNA metabarcoding can elucidate the presence of multiple mycetoma-causative fungi, which may contribute to accurate diagnosis for patient treatment and geographical mapping.
• Development and evaluation of PCR primers for environmental DNA (eDNA) metabarcoding of Amphibia
  Masayuki K. Sakata; Mone U. Kawata; Atsushi Kurabayashi; Takaki Kurita; Masatoshi Nakamura; Tomoyasu Shirako; Ryosuke Kakehashi; Kanto Nishikawa; Mohamad Yazid Hossman; Takashi Nishijima; Junichi Kabamoto; Masaki Miya; Toshifumi Minamoto
  Metabarcoding and Metagenomics, 6, 15, 26, Pensoft Publishers, 21 Feb. 2022, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal, Biodiversity monitoring is important for the conservation of natural ecosystems in general, but particularly for amphibians, whose populations are pronouncedly declining. However, amphibians’ ecological traits (e.g. nocturnal or aquatic) often prevent their precise monitoring. Environmental DNA (eDNA) metabarcoding – analysis of extra-organismal DNA released into the environment – allows the easy and effective monitoring of the biodiversity of aquatic organisms. Here, we developed and tested the utility of original PCR primer sets. First, we conducted in vitro PCR amplification tests with universal primer candidates using total DNA extracted from amphibian tissues. Five primer sets successfully amplified the target DNA fragments (partial 16S rRNA gene fragments of 160–311 bp) from all 16 taxa tested (from the three living amphibian orders Anura, Caudata and Gymnophiona). Next, we investigated the taxonomic resolution retrieved using each primer set. The results revealed that the universal primer set “Amph16S” had the highest resolution amongst the tested sets. Finally, we applied Amph16S to the water samples collected in the field and evaluated its detection capability by comparing the species detected using eDNA and physical survey (capture-based sampling and visual survey) in multiple agricultural ecosystems across Japan (160 sites in 10 areas). The eDNA metabarcoding with Amph16S detected twice as many species as the physical surveys (16 vs. 8 species, respectively), indicating the effectiveness of Amph16S in biodiversity monitoring and ecological research for amphibian communities.
• Universal performance of benzalkonium chloride for the preservation of environmental DNA in seawater samples
  Toshiaki Jo; Masayuki K. Sakata; Hiroaki Murakami; Reiji Masuda; Toshifumi Minamoto
  Limnology and Oceanography: Methods, 19, 11, 758, 768, Wiley, Nov. 2021, [Peer-reviewed]
  Scientific journal
• Environmental DNA preserved in marine sediment for detecting jellyfish blooms after a tsunami.
  Mizuki Ogata; Reiji Masuda; Hiroya Harino; Masayuki K Sakata; Makoto Hatakeyama; Katsuhide Yokoyama; Yoh Yamashita; Toshifumi Minamoto
  Scientific reports, 11, 1, 16830, 16830, 20 Aug. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Prevalence of antimicrobial-resistant Escherichia coli in migratory Greater White-fronted Goose (Anser albifrons) and their habitat in Miyajimanuma, Japan.
  Akira Fukuda; Masaru Usui; Katsumi Ushiyama; Dipti Shrestha; Nagisa Hashimoto; Masayuki K Sakata; Toshifumi Minamoto; Osamu Yoshida; Kanako Murakami; Yutaka Tamura; Tetsuo Asai
  Journal of wildlife diseases, 57, 4, 954, 958, 19 Aug. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, The spread of antimicrobial-resistant bacteria (ARB) in natural environments including wild animals is a concern for public health. Birds cover large areas, and some fly across borders to migrate in large flocks. As a migratory bird, the Greater White-fronted Goose (Anser albifrons) travels to Miyajimanuma, North Japan, each spring and autumn. To investigate the ARB in migratory birds and their surroundings, we collected 110 fecal samples of A. albifrons and 18 water samples from Miyajimanuma in spring and autumn of 2019. Isolation of Escherichia coli was performed using selective agars with or without antimicrobials (cefazolin and nalidixic acid). Isolates of E. coli were recovered from 56 fecal samples (50.9%) and five water samples (27.8%) on agars without antimicrobials. No isolates were recovered on agars with antimicrobials. One E. coli isolate derived from a fecal sample exhibited resistance to β-lactams (ampicillin and cefazolin), whereas all other isolates exhibited susceptibility to all tested antimicrobials. The resistant isolate harbored blaACC, which could be transferred to other bacteria and confer resistance to β-lactams. These results suggest a low prevalence of antimicrobial resistance in wild migratory birds and their living environments; however, wild migratory birds sometimes carry ARB harboring transferrable antimicrobial resistance genes and therefore present a risk of spreading antimicrobial resistance.
• Application of environmental DNA metabarcoding in a lake with extensive algal blooms
  Qianqian Wu; Masayuki K. Sakata; Deyi Wu; Hiroki Yamanaka; Toshifumi Minamoto
  LIMNOLOGY, 22, 3, 363, 370, Aug. 2021, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples.
  Tetsu Yasashimoto; Masayuki K Sakata; Tomoya Sakita; Satoko Nakajima; Mamiko Ozaki; Toshifumi Minamoto
  Scientific reports, 11, 1, 10712, 10712, 26 May 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Determining an effective sampling method for eDNA metabarcoding: a case study for fish biodiversity monitoring in a small, natural river
  Masayuki K. Sakata; Takeshi Watanabe; Nobutaka Maki; Kousuke Ikeda; Toshihiro Kosuge; Hiroaki Okada; Hiroki Yamanaka; Tetsuya Sado; Masaki Miya; Toshifumi Minamoto
  LIMNOLOGY, 22, 2, 221, 235, Apr. 2021, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Sedimentary DNA tracks decadal-centennial changes in fish abundance.
  Michinobu Kuwae; Hiromichi Tamai; Hideyuki Doi; Masayuki K Sakata; Toshifumi Minamoto; Yoshiaki Suzuki
  Communications biology, 3, 1, 558, 558, 08 Oct. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Sedimentary eDNA provides different information on timescale and fish species composition compared with aqueous eDNA
  Masayuki K. Sakata; Satoshi Yamamoto; Ryo O. Gotoh; Masaki Miya; Hiroki Yamanaka; Toshifumi Minamoto
  Environmental DNA, 2, 4, 505, 518, Wiley, Oct. 2020, [Peer-reviewed], [Lead author, Corresponding author]
  Scientific journal
• Effects of sampling seasons and locations on fish environmental DNA metabarcoding in dam reservoirs.
  Kana Hayami; Masayuki K Sakata; Takashi Inagawa; Jiro Okitsu; Izumi Katano; Hideyuki Doi; Katsuki Nakai; Hidetaka Ichiyanagi; Ryo O Gotoh; Masaki Miya; Hirotoshi Sato; Hiroki Yamanaka; Toshifumi Minamoto
  Ecology and evolution, 10, 12, 5354, 5367, Jun. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Environmental DNA monitoring for short‐term reproductive migration of endemic anadromous species, Shishamo smelt ( Spirinchus lanceolatus )
  Tetsu Yatsuyanagi; Ryotaro Ishida; Masayuki K. Sakata; Takashi Kanbe; Hiroki Mizumoto; Yumi Kobayashi; Shoko Kamada; Satoko Namba; Hisaya Nii; Toshifumi Minamoto; Hitoshi Araki
  Environmental DNA, 2, 2, 130, 139, Wiley, Apr. 2020, [Peer-reviewed]
  English, Scientific journal
• Comparing the efficiency of open and enclosed filtration systems in environmental DNA quantification for fish and jellyfish.
  Sayaka Takahashi; Masayuki K Sakata; Toshifumi Minamoto; Reiji Masuda
  PloS one, 15, 4, e0231718, 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Environmental DNA revealed the fish community of Hokkaido Island, Japan, after invasion by rainbow trout.
  Akio Imamura; Kana Hayami; Masayuki K Sakata; Toshifumi Minamoto
  Biodiversity data journal, 8, e56876, 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Estimations of Riverine Distribution, Abundance, and Biomass of Anguillid Eels in Japan and Taiwan Using Environmental DNA Analysis.
  Hikaru Itakura; Ryoshiro Wakiya; Masayuki K Sakata; Hsiang-Yi Hsu; Shih-Chong Chen; Chih-Chao Yang; Yi-Cheng Huang; Yu-San Han; Satoshi Yamamoto; Toshifumi Minamoto
  Zoological studies, 59, e17, 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Correction to: Dispersion and degradation of environmental DNA from caged fish in a marine environment (Fisheries Science, (2019), 85, 2, (327-337), 10.1007/s12562-018-1282-6)
  Hiroaki Murakami; Seokjin Yoon; Akihide Kasai; Toshifumi Minamoto; Satoshi Yamamoto; Masayuki K. Sakata; Tomoya Horiuchi; Hideki Sawada; Michio Kondoh; Yoh Yamashita; Reiji Masuda
  Fisheries Science, 85, 6, 1109, Springer Tokyo, 01 Nov. 2019
  English, Scientific journal
• Comparison of inhibition resistance among PCR reagents for detection and quantification of environmental DNA
  Kimiko Uchii; Hideyuki Doi; Teruyuki Okahashi; Izumi Katano; Hiroki Yamanaka; Masayuki K. Sakata; Toshifumi Minamoto
  Environmental DNA, 1, 4, 359, 367, Wiley, Nov. 2019, [Peer-reviewed]
  English, Scientific journal
• Habitat selection and migration of the common shrimp, Palaemon paucidens in Lake Biwa, Japan—An eDNA‐based study
  Qianqian Wu; Ken Kawano; Toshiyuki Ishikawa; Masayuki K. Sakata; Ryohei Nakao; Masayoshi K. Hiraiwa; Satsuki Tsuji; Hiroki Yamanaka; Toshifumi Minamoto
  Environmental DNA, 1, 1, 54, 63, Wiley, May 2019, [Peer-reviewed]
  English, Scientific journal, AbstractPalaemon paucidens has a large population and is an important food source for fish in Lake Biwa, Japan. They are abundant in shallow waters from spring to summer, after which most individuals migrate to offshore deep areas where they remain during autumn and winter. However, some individuals are nonmigratory, remaining in shallow waters over winter. It has been reported that P. paucidens individuals have declined in recent years; a better understanding of its seasonal distribution is needed to manage this species, and basic information on its seasonal distribution is indispensable. We tracked the environmental DNA (eDNA) distribution of P. paucidens in Lake Biwa over a year using a quantitative real‐time polymerase chain reaction method. We collected water samples from offshore (both from the surface and from the benthic) and from shallow shore sites adjacent to the shorelines of the main lake and connecting freshwater lagoons. Offshore sampling took place in summer and winter, and shallow shore and lagoon sampling in all four seasons. During summer, eDNA concentrations were significantly higher in shallow and lagoon areas than offshore bottom sites. Conversely, during winter, eDNA concentrations were higher in offshore bottom sites, and relatively high and low eDNA concentrations in lagoons and shallow shore, respectively. These results most likely reflect the spatial and temporal distribution of this species in Lake Biwa. The eDNA concentrations peaked in early August at shallow shore sites in the main lake, with a significant decline in mid‐October, while low eDNA concentrations were recorded at offshore bottom sites in late August. These results suggest that P. paucidens migrates from shallow waters to offshore bottom sites between early August and mid‐October. These results provide important information for the management of this species.
• Dispersion and degradation of environmental DNA from caged fish in a marine environment
  Hiroaki Murakami; Seokjin Yoon; Akihide Kasai; Toshifumi Minamoto; Satoshi Yamamoto; Masayuki K. Sakata; Tomoya Horiuchi; Hideki Sawada; Michio Kondoh; Yoh Yamashita; Reiji Masuda
  FISHERIES SCIENCE, 85, 2, 327, 337, Mar. 2019, [Peer-reviewed]
  English, Scientific journal
• Are Salvelinus species on Hokkaido Island, Japan, endangered by damming and invasive rainbow trout: Results from eDNA analysis
  Imamura Akio; Hayami Kana; Sakata Masayuki K.; Minamoto Toshifumi
  Japanese Journal of Conservation Ecology, 24, 1, 71, 81, The Ecological Society of Japan, 2019, [Peer-reviewed]
  Japanese, Scientific journal, We investigated the distributions of two native (Dolly Varden Salvelinus malma malma and whitespotted char Salvelinus leucomaenis leucomaenis) and one invasive (rainbow trout Oncorhynchus mykiss) salmonid species using environmental DNA (eDNA) analysis in the centre of Hokkaido Island, Japan. The native species' populations are fragmented by damming and threatened by invasive species. Therefore, DNA real-time PCR assays specific to these three salmonids were used to investigate the effects of damming and invasive species on the two native salmonids. Salvelinus malma populations exhibited separation due to damming. Additionally, they were not eliminated by invasive O. mykiss but rather lived together at some sites. Salvelinus leucomaenis populations occupied the lower reaches more than did S. malma populations. We detected S. leucomaenis and the invasive O. mykiss population less frequently than expected. We were unable to clarify the seasonal movements of species, even during their reproductive phase, despite conducting eDNA surveys throughout the year, including during the coldest parts of winter. We hypothesise that damming may function both as a protective barrier against invasive species and as an impassable barrier preventing migration; however, the significance of these potential functions was not revealed in this study. From a long-term perspective, fragmentation may negatively affect the viability of native Salvelinus populations. Conservation efforts for native Salvelinus species would be aided by additional studies using eDNA surveys, which can be effectively conducted even in mid-winter.
• Real-time polymerase chain reaction assays for environmental DNA detection of three salmonid fish in Hokkaido, Japan: Application to winter surveys
  Toshifumi Minamoto; Kana Hayami; Masayuki K. Sakata; Akio Imamura
  ECOLOGICAL RESEARCH, 34, 1, 237, 242, Jan. 2019, [Peer-reviewed]
  English, Scientific journal
• Detecting river fish using environmental DNA analysis and a conventional field sampling survey at 500-m intervals
  Niwa Hideyuki; Sakata Masayuki K.; Minamoto Toshifumi; Kiyono Mieko
  Japanese Journal of Conservation Ecology, 23, 2, 257, 264, The Ecological Society of Japan, 2018, [Peer-reviewed]
  Japanese, Environmental DNA (eDNA) analysis for macro-organisms has developed rapidly, and studies have reported the application of such analyses to various kinds of organism. However, only a few studies have compared the results of eDNA analysis and traditional sampling surveys on a fine scale, and the usefulness of eDNA analysis for understanding the distribution of endangered species on a finer scale has not been sufficiently assessed. In this study, we conducted eDNA analysis and a traditional sampling survey in multiple 500-m sections of a river and compared the results. The survey targets were the endangered fish species Liobagrus reinii, Coreoperca kawamebari, and Lethenteron sp. 2, which are known to occur in the surveyed river. We established 23, 500-m-long sections in the Sasayama River and sampled water at the downstream end of each section. The eDNA of the target species was detected using real-time PCR assays developed in this study. We also collected the target species in traditional sampling surveys and recorded the presence/absence of the target species in each section. The rates of concordance between the results of the eDNA analysis and the traditional sampling surveys varied among the species. Our result showed that the results of eDNA analysis and field sampling surveys do not necessarily match at a finer scale, and suggested the importance of knowledge accumulation for eDNA analysis for proper use of this analysis method to conserve endangered species.
• Identifying a breeding habitat of a critically endangered fish, Acheilognathus typus, in a natural river in Japan.
  Masayuki K Sakata; Nobutaka Maki; Hideki Sugiyama; Toshifumi Minamoto
  Die Naturwissenschaften, 104, 100, 14 Nov. 2017, [Peer-reviewed], [Lead author], [International Magazine]
  English, Scientific journal
• Rapid degradation of longer DNA fragments enables the improved estimation of distribution and biomass using environmental DNA.
  Toshiaki Jo; Hiroaki Murakami; Reiji Masuda; Masayuki K Sakata; Satoshi Yamamoto; Toshifumi Minamoto
  Molecular ecology resources, 17, 6, e25, e33, Nov. 2017, [Peer-reviewed], [International Magazine]
  English, Scientific journal

• 絶滅危惧種ニホンザリガニの個体群存続条件の可視化と保全への適用 ―北教大－神戸大 水環境チーム―
  今村 彰生; 源 利文; 坂田 雅之, 第33期プロ・ナトゥーラ・ファンド助成 国内研究助成, 34, 83, 90, May 2025
  Japanese
• 自由集会開催報告・企画者報告「水を汲むのはもう飽きた！〜水以外を媒体とする環境DNA分析〜」
  坂田雅之, 環境DNA学会 ニュースレター NO.6, Apr. 2024, [Invited], [Lead author]
  Japanese, Introduction other
• 環境DNA研究最前線（第5回）「北海道大学農学部動物生態学研究室の紹介」
  坂田雅之, 環境DNA学会 ニュースレター NO.5, Apr. 2023, [Invited], [Lead author]
  Japanese, Introduction other
• Species-specific detection for terrestrial insects through a soil sample
  Masayuki Sakata; Tetsu Yasashimoto, 昆虫と自然 2022年10月号 特 集・環境DNAは昆虫調査に使えるか？, Oct. 2022, [Lead author]
• Dispersion and degradation of environmental DNA from caged fish in a marine environment
  弘章 村上; 錫鎭 尹; 亮秀 笠井; 利文 源; 哲史 山本; 雅之 坂田; 智矢 堀内; 英樹 澤田; 倫生 近藤; 洋 山下; 玲爾 益田, NIPPON SUISAN GAKKAISHI, 88, 4, 206, 206, 15 Jul. 2022
  Japanese Society of Fisheries Science
• 自由集会開催報告・企画者報告「多様な分類群における環境DNA分析の現状と展望」
  坂田雅之, 環境DNA学会 ニュースレター NO.4, Mar. 2022, [Invited], [Lead author]
  Japanese, Introduction other
• Environmental DNA preservation in sediment with application to detecting jellyfish blooms after the tsunami
  尾形瑞紀; 益田玲爾; 張野宏也; 坂田雅之; 畠山信; 横山勝英; 山下洋; 源利文, 個体群生態学会大会プログラム・講演要旨集(Web), 36th, 2020
• Trials to visualize the effects of invasive rainbow trout on the native fish community in central Hokkaido, Japan ―HUE & Kobe U. Freshwater Biology Team―
  IMAMURA Akio; HAYAMI Kana; SAKATA Masayuki K.; MINAMOTO Toshifumi, Annual Report of Pro Natura Foundation Japan, 29, 162, 172, 2020
  

  We tried to reveal species composition and its seasonal change of the freshwater-fish community in the Kamikawa plain on Hokkaido Island, Japan, using meta-barcoding analysis of eDNA. We tried to detect negative effects of rainbow trout (Oncorhynchus mykiss) onto the native-fish community. Sixteen research sites were located at Ishikarigawa River system including a primary tributary, Chubetsu-gawa River. We collected water-samples once a month from Oct. 2018 to Aug. 2019. We detected DNAs of 36 ASVs (operational taxonomic units) from 12 families. The numbers of ASVs per site ranged 12–23. The DNAs of Barbatula barbatula, Misgurnus anguillicaudatus, Cottus nozawae, and rainbow trouts were detected at all the research sites. As a whole, the frequency and proportion of native species were high. As for native salmonids, DNAs of Masu salmon (Oncorhynchus masou sspp.), whitespotted char (Salvelinus leucomaenis sspp.), dolly varden (Salvelinus malma sspp.), and chum salmon (Oncorhynchus keta spp.) were detected at 15, 12, 11, and 11 sites, respectively. The number of ASVs per site was largest at Asahibashi bridge in the central Asahikawa City. The numbers of ASVs detected were large at the sites of tributaries of Ishikari-gawa River, i.e. Masutori-gawa and Piukenai. The sites around the Chubetsu Reservoir tended to contain smaller numbers of ASVs detected. The sites at the lower reaches of the Ishikari-gawa and Chubetsu-gawa Rivers tended to contain richer number of species including large numbers of Cyprinidae species. We did not detect the exclusive relationship between rainbow trout and the native salmonid species.

  , Pro Natura Foundation Japan, Japanese

• 環境DNA : 生態系の真の姿を読み解く−（土居秀幸・近藤倫生編）
  坂田雅之; 徐寿明; 源利文, pp. 1-35. 第1章「環境DNA分析の概要」
  共立出版, Mar. 2021, 9784320058163, x, 281p, 図版 [8] p, Japanese, [Contributor]

• e-エピジェネティックスで切り開く環境DNA研究の最前線
  坂田雅之; 平山一槻; 源利文; 荒木仁志
  第73回日本生態学会大会, Mar. 2026, Japanese, Nominated symposium
  [Invited]
• 耕作放棄がセトウチサンショウウオを脅かす -生息への影響と保全管理の提案-
  松本奈々; 坂田雅之; 國政祐太; 山本優奈; 源利文
  第73回日本生態学会大会, Mar. 2026, Japanese, Poster presentation
  46475759
• The novel identification method of the hybrid using environmental DNA
  Sakata M. K.; Yano N.; Imamura A.; Yamanaka H.; Minamoto T.
  BES Annual Meeting 2025, Dec. 2025, English, Poster presentation
  44306553
• Environmental DNA dynamics during early development and physiological changes in salmon
  Masayuki K. Sakata; Tahashi Kanbe; Hirotoshi Sato; Hitoshi Araki
  8th Annual Meeting of The eDNA Society, Dec. 2025, English, Poster presentation
• 野外環境水からの細胞核回収法の確立および分析手法の最適化：交雑種検出への応用
  矢野 七虹; 坂田 雅之; 今村 彰生; 山中 裕樹; 源 利文
  第8回環境DNA学会, Dec. 2025, Japanese, Poster presentation
  44306553
• 環境 DNA を用いた北日本沿岸におけるアイナメ属分布推定
  福山 享; 坂田 雅之; 神戸 崇; 井上 頌子; 八柳 哲; 荒木 仁志
  第8回環境DNA学会, Dec. 2025, Japanese, Poster presentation
• 耕作放棄がセトウチサンショウウオ（Hynobius setouchi）の生息に及ぼす影響の解明
  松本奈々; 坂田雅之; 國政祐太; 山本優奈; 源利文
  日本陸水学会大89回大会札幌大会, Sep. 2025, Japanese, Oral presentation
  46475759
• The novel identification method of the hybrid using environmental DNA.
  Sakata M. K.; Yano N.; Imamura A.; Yamanaka H.; Minamoto T.
  The 11th EAFES International Congress, Jul. 2025, English, Poster presentation
  44306553
• Impact of farmland abandonment on survival of a small salamander (Hynobius setouchi) using environmental DNA analysis.
  Matsumoto N.; Sakata M.K.; Kunimasa Y.; Yamamoto Y.; Minamoto T.
  The 11th EAFES International Congress, Jul. 2025, English, Poster presentation
• Environmental DNA can detect the hybrid
  Sakata M K.; Yano Nanako; Imamura Akio; Yamanaka Hiroki; Minamoto Toshifumi
  2nd Australian and New Zealand Environmental DNA Conference, Feb. 2025, English, Poster presentation
• Negative effects of farmland abandonment on the reproduction of a small salamander (Hynobius setouchi)
  Matsumoto Nana; Sakata Masayuki K.; Minamoto Toshifumi
  2nd Australian and New Zealand Environmental DNA Conference, Feb. 2025, English, Poster presentation
  46475759
• サケの初期発生段階と生理状態の変化に伴う環境DNA動態
  坂田雅之; 神戸崇; 佐藤俊平; 荒木仁志
  第17回サケ学研究会（盛岡）, Dec. 2024, Japanese, Oral presentation
• 環境DNAによる交雑の検出
  坂田雅之; 矢野七虹; 今村彰生; 山中裕樹; 源利文
  第7回環境DNA学会つくば大会, Dec. 2024, Japanese, Nominated symposium
  44306553, [Invited]
• 環境 DNA を用いた結氷期の厚岸湖における魚類相推定
  西谷航平; 坂田雅之; 神戸崇; 小林由美; 荒木仁志
  第7回環境DNA学会つくば大会, Dec. 2024, English, Poster presentation
• 北海道積丹半島に生息するヨシノボリ属2種の交雑とミトコンドリア遺伝子浸透
  坂井恭佳; 神戸崇; 坂田雅之; 荒木仁志
  第7回環境DNA学会つくば大会, Dec. 2024, English, Poster presentation
• 耕作放棄地におけるセトウチサンショウウオの分布を規定する要因の推定
  松本奈々; 坂田雅之; 國政祐太; 山本優奈; 源利文
  第7回環境DNA学会つくば大会, Dec. 2024, English, Poster presentation
  46475759
• 樹幹流を用いた環境DNAメタバーコーディングによる哺乳類相モニタリング法の検討
  石原湧也; 青木香澄; 坂田歩美; 宮正樹; 浜野杏平; 坂田雅之; 邬倩倩; 源利文
  第7回環境DNA学会つくば大会, Dec. 2024, English, Poster presentation
• メコン住血吸虫の環境ＤＮＡ検出系の開発と野外への応用
  松村奈桜; 松尾莉子; サトウ恵; Marcello Otake Sato; Joseph Evangelista Valencia; Mark June Valiente; Revolteado Phoyphaylinh Prasayasith; Poom Adisakwattana; Yanin Limpanon; Orawan Phuphisu; Tippayarat Yoonuan; Somphou Sayasone; 坂田雅之; 邬倩倩; 源利文
  第7回環境DNA学会つくば大会, Dec. 2024, English, Poster presentation
• 環境DNAを用いたセトウチサンショウウオ(Hynobius setouchi)の生息に影響する環境要因の推定
  松本奈々; 坂田雅之; 國政祐太; 山本優奈; 源利文
  応用生態工学会 第２７回さいたま大会, Sep. 2024, Japanese, Poster presentation
  46475759
• Estimating suitable breeding habitat for the Setouchi salamander (Hynobius setouchi) based on environmental DNA surveys
  Nana Matsumoto; Masayuki K. Sakata; Toshifumi Minamoto
  Salamander Meeting 2024, English, Poster presentation
  31 Jul. 2024 - 02 Aug. 2024
• 堆積物・土壌環境DNAの概要と紹介
  坂田雅之
  第6回環境DNA学会九州大会, Dec. 2023, Japanese, Public symposium
  38573820, [Invited]
• セトウチサンショウウオの繁殖環境に関する研究
  久富早織; 坂田雅之; 中村仁湖; 源利文
  第6回環境DNA学会九州大会, Dec. 2023, Japanese, Poster presentation
• 河川における在来魚類と外来魚類の分布状況について
  太田井麻令乃; 橋本渚; 國政祐太; 平山一槻; 山本義彦; 坂田雅之; 源利文
  第6回環境DNA学会九州大会, Dec. 2023, Japanese, Poster presentation
• 環境DNA分析と生態モデルによる気候変動下における生物の時空間分布予測
  邬倩倩; 周金鑫; 河本達也; 石川俊之; 後藤直成; 坂田雅之; 北澤大輔; 源利文
  第6回環境DNA学会九州大会, Dec. 2023, Japanese, Poster presentation
• 高濁度水サンプルからの環境核酸抽出方法の検討
  山本優奈; 岩本遼; 平山一槻; 小川あゆみ; 坂田雅之; 源利文
  第6回環境DNA学会九州大会, Dec. 2023, Japanese, Poster presentation
• 環境DNAメタバーコーディング手法を用いたため池における水生植物のモニタリング
  小川あゆみ; 久富早織; 橋本渚; 坂田雅之; 源利文
  第6回環境DNA学会九州大会, Dec. 2023, Japanese, Poster presentation
• メコン住血吸虫の環境ＤＮＡ検出系の開発と野外への応用
  松村奈桜; 松尾莉子; 坂田雅之; 邬倩倩; 源利文
  第6回環境DNA学会九州大会, Dec. 2023, Japanese, Poster presentation
• 環境DNAを用いた陸上哺乳類と鳥類の検出方法の開発
  中村仁湖; 橋本渚; 坂田雅之; 邬倩倩; 源利文
  第6回環境DNA学会九州大会, Oct. 2023, Japanese, Public symposium
  [Invited]
• 堆積物DNAを用いた阿寒湖における魚類個体群動態の復元
  坂田雅之; 加三千宣; 大槻朝; 若菜勇; 源利文; 占部城太郎
  日本陸水学会第87回大分大会, Oct. 2023, Japanese, Oral presentation
• 環境DNAを用いて推定された外来ミンク個体群の定着と人間活動の関係
  坂田雅之; 高羽俊宏; 神戸崇; 三塚多佳志; 井上頌子; 水本寛基; 荒木仁志
  日本哺乳類学会2023年度大会, Sep. 2023, Japanese, Poster presentation
• Fish population dynamics in Lake Akan associated with eutrophication reconstructed by sedimentary DNA
  SakataM. K; Kuwae M; Ohtsuki H; Wakana I; Minamoto T; Urabe J
  SedaDNA Meeting Potsdam, Jun. 2023, English, Poster presentation
  38573820
• Seasonal movement of Rainbow Trout detected by year-round water sampling
  Imamura A; Sakata M. K; Minamoto T
  The eDNA Society International Meeting 2023, May 2023, English, Poster presentation
• Fish population dynamics in Lake Akan associated with eutrophication reconstructed by sedimentary DNA
  SakataM. K; Kuwae M; Ohtsuki H; Wakana I; Minamoto T; Urabe J
  The eDNA Society International Meeting 2023, May 2023, English, Poster presentation
• シカのヌタ場が両生類の繁殖に与える影響
  松浦なる; 岡宮久規; 八柳哲; 坂田雅之; 岸田治; 荒木仁志
  日本生態学会第70回全国大会, Mar. 2023, Japanese, Poster presentation
• Environmental DNA in lake sediment provides fish information on reconstructing past fauna in lake ecosystems
  Masayuki K. Sakata; Narumi Tsugeki; Michinobu Kuwae; Hideyuki Doi; Toshifumi Minamoto
  BES Annual Meeting 2022, 20 Dec. 2022, English, Poster presentation
  18 Dec. 2022 - 21 Dec. 2022
• 堆積物中に含まれる環境DNAの応用可能性
  坂田雅之
  環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese, Poster presentation
  38573820;33705442
• 環境DNAを用いた陸上哺乳類の検出方法の開発
  中村仁湖; 橋本渚; 坂田雅之; 鄔倩倩; 源利文
  環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese, Poster presentation
• スナメリの検出系開発と野外適用
  橋本渚; 木原菜摘; 坂田雅之; 中村清美; 源利文; 岩田高志
  環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese, Poster presentation
• 各組織が分散してデータを管理し、段階的にデータを共有・公開してデータの価値を高め活用する「データ価値共創環境」の提案
  坂本久; 庄司諒; 川北智弥; 坂田雅之; 山中裕樹; 源利文
  環境DNA学会「あなたが主役のワークショップ」, Nov. 2022, Japanese, Poster presentation
• 堆積物環境DNAによる琵琶湖魚類の過去復元
  坂田雅之; 槻木玲美; 加三千宣; 土居秀幸; 源利文
  日本陸水学会第86回大会, Sep. 2022, Japanese, Oral presentation
  38573820;33705442
• 堆積物コアDNAを用いた宍道湖におけるマクロ生物の近過去生息状況の推定
  山岸聖; 小室隆; 神門利之; 引野愛子; 坂田雅之; 源利文; 下田莉奈; 高原輝彦
  2022年度生物系三学会中国四国地区合同大会, May 2022, Japanese, Oral presentation
• Seda DNAを用いた宍道湖における過去の車軸藻類の復元
  小室隆; 神門利之; 加藤季晋; 引野愛子; 山岸聖; 高原輝彦; 後藤益滋; 坂田雅之; 源利文
  2022年日本地理学会春季学術大会, Mar. 2022, Oral presentation
• 環境DNA分析を用いた薬剤耐性菌の保菌動物の探索
  橋本渚; 坂田雅之; 杉山美千代; 浅井鉄夫; 源利文
  第69回日本生態学会大会, Mar. 2022, Poster presentation
• 琵琶湖における廃川の魚類ハビタットとしての活用可能性 」第69回日本生態学会大会
  東坂波也翔; 松本岳大; 坂田雅之; 源利文
  第69回日本生態学会大会, Mar. 2022, Poster presentation
• 多種サイト占有モデルを用いた環境DNA分析における調査デザインの最適化
  松本岳大; 深谷肇一; 坂田雅之; 沖津二朗; 稲川崇史; 平岡康介; 一柳英隆; 源利文
  第69回日本生態学会大会, Mar. 2022, Poster presentation
• 核DNAをマーカーとする魚類環境DNAメタバーコーディング法の野外適用
  佐々木大介; 伊藤玄; 山中裕樹; 坂田雅之; 源利文
  第69回日本生態学会大会, Mar. 2022, Poster presentation
• 流水性希少サンショウウオを対象にした効率的な環境DNA検出手法の検討
  坂田雅之; 竹下大輝; 西澤崚平; 佐藤拓哉; 源利文
  第69回日本生態学会大会, Mar. 2022, Poster presentation
• 全循環湖から部分循環湖への変化が底生生物に与える影響について
  邬倩倩; 周金鑫; 河本達也; 石川俊之; 坂田雅之; 後藤直成; 北澤大輔; 源利文
  第69回日本生態学会大会, Mar. 2022, Poster presentation
• 海洋堆積物のDNA量から魚類個体数の数十年スケール変動を捉えられるか
  加三千宣; 玉井弘道; 土居秀幸; 源利文; 坂田雅之
  2021年度水産海洋学会研究発表大会, Nov. 2021, Oral presentation
• Development and evaluation of PCR primers for environmental DNA metabarcoding of Amphibia
  坂田雅之; 河田萌音; 倉林敦; 栗田隆気; 中村匡聡; 白子智康; 掛橋竜祐; 西川完途; モハマド=ヤジッド=ホスマン; 西島太加志; 樺元淳一; 宮正樹; 源利文
  環境DNA学会第4回大会, Nov. 2021, Japanese, Poster presentation
• Attempt of fish eDNA metabarcoding in a lake with algal blooms
  邬 倩倩; 坂田 雅之; 吴 德意; 山中 裕樹; 源 利文
  環境DNA学会第4回大会, Nov. 2021, Japanese, Poster presentation
• 既知コピー数DNAから得た閾値を用いた環境DNA解析の精度向上
  大﨑優介; 渡邊和紀; 米川侑希; 中澤聡; 海野洋敬; 西山依里; 村上 博昭; 松平崇弘; 坂田 雅之; 源利文
  環境DNA学会第4回大会, Nov. 2021, Japanese, Poster presentation
• Towards the implementation of environmental DNA metabarcoding for fish surveys in dam reservoirs
  松本岳大; 深谷肇一; 坂田雅之; 稲川崇史; 沖津二朗; 平岡康介; 一柳英隆; 源利文
  環境DNA学会第4回大会, Nov. 2021, Japanese, Poster presentation
• Development of eDNA metabarcoding assay targeting nuclear DNA as a marker for fish
  佐々木大介; 山中裕樹; 坂田雅之; 源利文
  環境DNA学会第4回大会, Nov. 2021, Japanese, Poster presentation
• 堆積物環境DNAを用いた琵琶湖における過去生物情報の再構築
  坂田雅之; 槻木玲美; 加三千宣; 土居秀幸; 源利文
  日本生態学会第68回大会, Mar. 2021, Poster presentation
• 環境DNAを用いたバラタナゴ属3種の同時検出系確立と野外適用
  木原菜摘; 坂田雅之; 源利文
  日本生態学会第68回大会, Mar. 2021, Poster presentation
• アオコの発生した湖におけるeDNA メタバーコーディング: 中国太湖を事例として
  邬倩倩; 坂田雅之; 吴德意; 山中裕樹; 源利文
  日本生態学会第68回大会, Mar. 2021, Poster presentation
• 琵琶湖の湖底貧酸素化に伴うスジエビ及びイサザへの影響について
  河本達也; 鄔倩倩; 坂田雅之; 石川俊之; 後藤直成; 源利文
  日本生態学会第68回大会, Mar. 2021, Poster presentation
• 環境DNAメタバーコーディングを用いたトンボ目多様性の評価
  矢指本哲, 坂田雅之, 中尾遼平, 源利文
  日本生態学会第68回大会, Mar. 2021, Poster presentation
• ダム湖において環境DNAメタバーコーディング手法を用いる際の最適採水地点数の検討
  松本岳大; 深谷肇一; 坂田雅之; 稲川崇史; 沖津二朗; 平岡康介; 一柳英隆; 源利文
  日本生態学会第68回大会, Mar. 2021, Poster presentation
• 環境DNA技術の創始者達が「黎明期」を語り、環境DNA技術の未来を占う
  源 利文; 山中 裕樹; 高原輝彦; 坂田 雅之
  環境DNA学会第3回大会・第36回個体群生態学会大会合同大会, Nov. 2020, Nominated symposium
  [Invited]
• 環境DNAメタバーコーディングを用いた魚類調査のための効果的なサンプリング方法の検討
  坂田雅之; 渡部健; 真木伸隆; 池田幸資; 小菅敏裕; 岡田泰明; 池田幸資; 山中裕樹; 佐土哲也; 宮正樹; 源利文
  環境DNA学会第3回大会・第36回個体群生態学会大会合同大会, Nov. 2020, Poster presentation
• 堆積物中における環境DNAの残存性とこれを応用した津波後のクラゲ類ブルームの検出
  尾形瑞紀; 益田玲爾; 張野宏也; 坂田雅之; 畠山信; 横山勝英; 山下洋; 源利文
  環境DNA学会第3回大会・第36回個体群生態学会大会合同大会, Nov. 2020, Poster presentation
• メタバーコーディングによる北海道の在来魚群集とニジマスの分布把握の試み
  今村彰生; 速水花奈; 坂田雅之; 源利文
  第67回日本生態学会, Mar. 2020, Poster presentation
• トンボ目環境DNAメタバーコーディングの野外水系での適用
  矢指本哲; 坂田雅之; 山添寛治; 源利文
  第67回日本生態学会, Mar. 2020, Poster presentation
• 哺乳類環境DNAメタバーコーディング手法による薬剤耐性菌保菌動物の推定
  速水花奈; 坂田雅之; 荒谷朋紀; 浅井鉄夫; 源利文
  第67回日本生態学会, Mar. 2020, Poster presentation
• 琵琶湖における堆積物環境DNAを用いた過去情報の復元
  坂田雅之; 槻木玲美; 加三千宣; 越智梨月; 速水花奈; 源利文
  第67回日本生態学会, Mar. 2020, Poster presentation
• Sedimentary eDNA provides different information on timescale and fish species composition compared with aqueous eDNA
  Sakata M. K; Yamamoto S; Gotoh R. O; Miya M; Yamanaka H; Minamoto T
  British Ecological Society Annual Meeting 2019, Dec. 2019, English, Poster presentation
• ダム湖における魚類環境DNAの鉛直分布
  速水花奈; 坂田雅之; 沖津二朗; 稲川崇史; 源利文
  第2回環境DNA学会神戸大会, Nov. 2019, Poster presentation
• ニジマスと在来イワナは共存できるのか?
  坂田雅之; 源利文; 速水花奈; 今村彰生
  第2回環境DNA学会神戸大会, Nov. 2019, Nominated symposium
  [Invited]
• Application of bamboo biomass resources in agrochemical-free rice farming: effects on Odonata diversity
  Thien Quang Huynh; Masayuki K. Sakata; Ryohei Nakao; Shinya Nomura; Masfiro Lailati; Toshifumi Minamoto; Nisikawa Usio
  日本陸水学会大84回大会, Sep. 2019, English, Oral presentation
• 環境 DNA メタバーコーディングによる希少淡水魚ゼニタナゴと関連生物の探索
  坂田雅之; 土居秀幸; 真木伸隆; 上田夏希; 杉山秀樹; 源利文
  日本陸水学会大84回大会, Sep. 2019, Oral presentation
• 採水量・採水位置の違いによる小出川における魚類相の検出精度について
  真木伸隆; 渡部健; 岡田泰明; 小菅敏裕; 池田幸資; 松島夕佳子; 坂田雅之; 源 利文
  土木学会第74回年次学術講演会, Sep. 2019, Oral presentation
• 流域の⿂類相調査における環境DNAメタバーコーディング分析法の有効性について（神奈川県・⼩出川の事例報告）
  加藤敦子; 岡田泰明; 渡部健; 真木伸隆; 小菅敏裕; 池田幸資; 松島夕佳子; 坂田雅之; 源利文
  土木学会第74回年次学術講演会, Sep. 2019, Oral presentation
• 堆積物環境DNAの過去復元への応用可能性
  坂田雅之; 源利文
  日本進化学会第21回大会, Aug. 2019, Nominated symposium
  [Invited]
• 堆積物からの環境DNA抽出法の効率化
  坂田雅之; 源利文
  第66回日本生態学会大会, Mar. 2019, Poster presentation
• 陸上土壌サンプルに由来する外来アリ環境DNAの検出
  矢指本哲; 坂田雅之; 先田智也; 尾崎まみこ; 中嶋智子; 源利文
  第66回日本生態学会大会, Mar. 2019, Poster presentation
• 水及び土壌由来環境DNAを用いた哺乳類相の推定
  速水花奈; 坂田雅之; 源利文
  第66回日本生態学会大会, Mar. 2019, Poster presentation
• 環境DNA分析法を用いた琵琶湖におけるスジエビの動態の解明
  邬倩倩; 河野健; 石川俊之; 坂田雅之; 中尾遼平; 平岩将良; 辻冴月; 山中裕樹; 源利文
  第66回日本生態学会大会, Mar. 2019, Poster presentation
• 環境DNAから探る、日本固有種シシャモの遡上と分布
  八柳哲; 神戸崇; 水本寛基; 小林由美; 坂田雅之; 源利文; 石田良太郎; 新居久也; 荒木仁志
  第66回日本生態学会大会, Mar. 2019, Poster presentation
• メタバーコーディング解析を用いた両生類の環境DNA検出
  河田萌音; 倉林淳; RAMAMONJISOA Noerikanto; 夏原由博; 山中裕樹; 佐藤博俊; 坂田雅之; 源利文
  第66回日本生態学会大会, Mar. 2019, Poster presentation
• Application of bamboo biomass resources in agrochemical-free rice farming: effects on odonate diversity
  HUYNH Thien Quang; SAKATA Masayuki K; NAKAO Ryohei; MINAMOTO Toshifumi; LAILATI Masfiro; USIO Nisikawa
  第66回日本生態学会大会, Mar. 2019, English, Poster presentation
• 環境DNAメタバーコーディング手法を用いたダム湖の魚類相把握
  速水花奈; 坂田雅之; 沖津二朗; 片野泉; 宮正樹; 後藤亮; 佐藤博敏; 山中裕樹; 源利文
  応用生態工学会仙台東北地域研究発表・シンポジウム, Nov. 2018, Poster presentation
• 堆積物由来環境DNA抽出法の改善と過去復元への展望
  坂田雅之; 源利文
  日本陸水学会第83回大会, Oct. 2018, Oral presentation
• トンボ目を対象とした環境DNAメタバーコーディング検出系の開発
  坂田雅之; 内田圭; 佐藤博俊; 山中裕樹; 中尾遼平; 源利文
  第1回環境DNA学会東京大会, Sep. 2018, Poster presentation
• 環境DNAを用いた北海道固有種シシャモSpirinchus lanceolatusの河川遡上パターン検出
  八柳哲; 神戸崇; 水本寛基; 小林由美; 鎌田頌子; 南波聡子; 新居久也; 源利文; 坂田雅之; 荒木仁志
  第1回環境DNA学会東京大会, Sep. 2018, Poster presentation
• ダム湖における魚類環境DNAメタバーコーディング手法の最適化
  速水花奈; 坂田雅之; 沖津二朗; 片野泉; 宮正樹; 後藤亮; 佐藤博俊; 山中裕樹; 源利文
  第1回環境DNA学会東京大会, Sep. 2018, Poster presentation
• Maximizing the potential of environmental DNA metabarcoding for fish detection in lentic ecosystem
  MINAMOTO Toshifumi; HAYAMI Kana; SAKATA Masayuki K; OKITSU Jiro; MIYA Masaki; GOTOH Ryo O; SATO Hirotoshi; YAMANAKA Hiroki
  ASLO 2018 Summer Meeting, Jun. 2018, English, Poster presentation
• 環境DNA分析を用いたため池の生物多様性を規定する要因の解明 −トンボ類および魚類を用いた事例−
  坂田雅之; 内田圭; 佐藤博俊; 山中裕樹; 中尾遼平; 源利文
  日本生態学会第65回全国大会, Mar. 2018, Poster presentation
• 北海道のイワナ属はニジマスと共存できるか～環境 DNA を用いた３種の分布データをもとに～
  速水花奈; 坂田雅之; 今村彰生; 源利文
  日本生態学会第65回全国大会, Mar. 2018, Poster presentation
• Identifying a breeding habitat of a critically endangered fish, Acheilognathus typus, by combining eDNA and conventional surveys
  T. Minamoto; MK. Sakata; N. Maki; H. Sugiyama
  Ecology Across Borders: Joint Annual Meeting 2017, Dec. 2017, English, Poster presentation
• 雄物川本流における絶滅危惧種ゼニタナゴの再発見と繁殖地特定
  坂田雅之; 真木伸隆; 杉山秀樹; 源利文
  日本陸水学会第82回大会, Sep. 2017, Oral presentation
• 希少生物調査における環境DNA手法の有効性の再確認-環境DNAを用いたゼニタナゴ新規繁殖地の発見
  真木伸隆; 坂田雅之; 杉山秀樹; 源利文; 土岐君仁
  土木学会第72回年次学術講演会, Sep. 2017, Oral presentation
• Longer DNA fragments enables the improved estimation of distribution and biomass using environmental DNA
  T. Minamoto; T. Jo; H. Murakami; R. Masuda; MK. Sakata; S. Yamamoto
  2017 ESA Annual Meeting, Aug. 2017, English, Poster presentation
• 雄物川本流における稀少淡水魚ゼニタナゴの再発見
  坂田雅之; 真木伸隆; 杉山秀樹; 源利文
  日本生態学会第64回全国大会, Mar. 2017, Poster presentation
• 兵庫県篠山市の河川における希少魚種の環境DNA検出
  河野健; 清野未恵子; 丹羽英之; 篠谷和彦; 田井彰人; 坂田雅之; 源利文
  日本生態学会第64回全国大会, Mar. 2017, Poster presentation
• 堆積物中の環境DNAを用いた魚類DNAのメタバーコーディング
  坂田雅之; 山本哲史; 宮正樹; 源利文
  日本陸水学会第81回大会, Nov. 2016, Oral presentation
• 堆積物からの環境DNA検出
  坂田雅之; 山本哲史; 源利文
  日本生態学会第63回全国大会, Mar. 2016, Poster presentation
• 環境DNAの断片長による見た目の分解速度の違い
  徐寿明; 村上弘章; 坂田雅之; 益田玲爾; 山本哲史; 源利文
  日本生態学会第63回全国大会, Mar. 2016, Poster presentation
• 沿岸海域における環境DNAの分散過程に関する生簀を用いた検証実験
  村上弘章; 尹錫鎭; 笠井亮秀; 源利文; 山本哲史; 坂田雅之; 堀内智矢; 澤田英樹; 益田玲爾
  平成28年度日本水産学会春季大会, Mar. 2016, Oral presentation

• 生態進化学特論, 2024年, 修士課程, 農学院
• 生物生態・体系学特論Ⅱ, 2024年, 修士課程, 農学院
• 生物多様性学, 2024年, 学士課程, 農学部
• 国際交流Ⅰ, 2024年, 学士課程, 国際本部
• 一般教育演習(ﾌﾚｯｼｭﾏﾝｾﾐﾅｰ), 2024年, 学士課程, 全学教育
• 生物学Ⅱ, 2024年, 学士課程, 全学教育

• 環境DNA学会
• 日本陸水学会
• 日本生態学会

• 超高感度環境ＤＮＡマーカーを用いた過去復元の精緻化
  科学研究費助成事業
  Jun. 2025 - Mar. 2028
  源 利文; 坂田 雅之; 平山 一槻
  日本学術振興会, 挑戦的研究(萌芽), 神戸大学, Coinvestigator, 25K22493
• エピジェネティッククロックと環境DNAに基づく魚類個体群の齢構成・繁殖推定
  環境研究総合推進費
  Apr. 2025 - Mar. 2028
  環境省／環境再生保全機構, 環境問題対応型研究（ミディアムファンディング枠）, Coinvestigator, 4MF-2503
• 環境DNA手法を利用したタイ肝吸虫撲滅を目指したワンヘルス・エコヘルス対策
  科学研究費助成事業
  Apr. 2024 - Mar. 2027
  サトウ 恵; 立木 佑弥; 源 利文; オオタケサトウ マルセロ; 坂田 雅之
  日本学術振興会, 基盤研究(B), 新潟大学, Coinvestigator, 24K03089
• 環境核酸を用いた絶滅危惧種イトウの有効集団サイズ・遺伝子発現推定技術の開発と実践
  科学研究費助成事業
  30 Jun. 2023 - 31 Mar. 2026
  荒木 仁志; 坂田 雅之
  日本学術振興会, 挑戦的研究(萌芽), 北海道大学, 23K18145
• Detection of interspecific hybridization by eDNA analysis
  Grants-in-Aid for Scientific Research
  01 Apr. 2023 - 31 Mar. 2026
  源 利文; 山中 裕樹; 坂田 雅之
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Kobe University, 23H02556
• 北海道固有の魚シシャモの遡上河川及び環境選好性の解明
  研究助成（奨励）
  Aug. 2024 - Mar. 2025
  秋山記念生命科学振興財団, Principal investigator
• 小型サンショウウオ類の水域・陸域における分布規定要因の解明
  サステイナブルな未来への研究助成【提案研究コース】
  Apr. 2023 - Mar. 2025
  坂田雅之; 佐藤拓也; 源利文
  旭硝子財団, Principal investigator
• 堆積物DNAによる過去復元を用いた環境変化に伴う食物網動態の解明
  科学研究費助成事業 若手研究
  Apr. 2022 - Mar. 2025
  坂田 雅之
  日本学術振興会, 若手研究, 神戸大学, Principal investigator, 22K15183
• 堆積物中の環境ＤＮＡを用いた魚類相変遷の観測
  科学研究費助成事業 特別研究員奨励費
  Apr. 2019 - Mar. 2021
  坂田 雅之
  本研究では生物から環境中に放出されたDNAである環境DNAを検出する環境DNA分析手法を用いて、過去の水中堆積物に含まれる魚類のDNAを検出することで、魚類の近過去復元を行うことを目的としている。
  昨年度は8月に調査地である琵琶湖にて堆積物柱状試料を重力式コアサンプラーにより採取し、過去の堆積物を採取した（長さ約30cm）。採取した堆積物試料は1cmスライスに分けられそれぞれからDNAの抽出、クロロフィルaの濃度測定、帯磁率の測定を行った。サンプリングに並行してDNAの抽出法の改善も行い、既存の手法よりも感度を向上させることに成功している。昨年度の過去堆積物からのDNA抽出には改善した手法を適用した。琵琶湖に生息するアユとイサザを対象にリアルタイムPCR法を用いて過去のDNAの検出を試みたところ、両者において数十年以上の時間スケールでの検出に成功した。また、検出系が設計されていなかったため、イサザについては種特異的な検出系を作成して適用を行った。検出されたDNA量は時系列解析に基づきその量変動を推定した。アユについては断片的ではあるが過去のCPUEの記録が存在するため、本研究で得られたDNA量の変動と、CPUEの変動を比較したところ、有意な正の相関が得られた。この結果は、過去の堆積物からDNAを検出し、その変動を観測することで過去の魚類の生物量の変動を復元できる可能性があることを示唆している。これまでの近過去復元はプランクトン類などの遺骸が堆積物中に残りやすい分類群に対してはよく行われているが、魚類のような遺骸の残りにくい種に対しての実証はほとんどなく、本研究によって新たな近過去復元の可能性を示すことに成功した。
  日本学術振興会, 特別研究員奨励費, 神戸大学, Principal investigator, 19J11126