博士（理学）, The University of Tokyo, Mar. 2006

バイオイメージング

進化発生生物学

植物発生生物学

植物細胞生物学

Life Science, Plant molecular biology and physiology

Bachelor's degree program, School of Science

Master's degree program, Graduate School of Life Science

Doctoral (PhD) degree program, Graduate School of Life Science

Aug. 2017, 日本植物細胞分子生物学会, 奨励賞
NARAMOTO Satoshi

Jan. 2017, 東北大学生命科学研究科, 東北大学生命科学研究科奨励賞
International academic award, Japan

Dec. 2016, 東北植物学会, 東北植物学会奨励賞
Japan society, Japan

Abscisic acid signaling regulates primary plasmodesmata density for plant cell-to-cell communication.
Chiyo Jinno; Ken Fujisaki; Izumi Yotsui; Motoki Ouchi; Prerna Singh; Satoshi Naramoto; Daisuke Takezawa; Yoichi Sakata; Tomomichi Fujita
Science advances, 11, 19, eadr8298, 09 May 2025, [International Magazine]
English, Scientific journal, Cell-to-cell communication is essential for multicellular organisms. Plasmodesmata (PD) are plant-specific nanopore structures pivotal for cell-to-cell communication and plant survival. However, how PD form and their structure, regulation, and evolution remain largely unknown. Here, we demonstrate that the exogenous supply of abscisic acid (ABA), a well-conserved phytohormone in land plants, reduces primary PD density in the moss Physcomitrium patens. This regulation requires all core components of the ABA signaling pathway. Furthermore, we reveal that ABA-INSENSITIVE 5, a well-conserved transcription factor in the ABA signaling pathway of land plants, plays a pivotal role in PD density regulation, whereas ABA-INSENSITIVE 3 does not. Our findings show that the ABA-induced reduction in primary PD density is mediated by these ABA-responsive factors in P. patens. Considering previous reports on ABA-dependent PD regulation in both moss and angiosperms, we propose that the ABA-mediated control of PD biogenesis and permeability represents a conserved mechanism in land plants, with critical implications for cell-to-cell communication and stress adaptation.

Contrasting and conserved roles of NPR pathways in diverged land plant lineages.
Hyung-Woo Jeon; Hidekazu Iwakawa; Satoshi Naramoto; Cornelia Herrfurth; Nora Gutsche; Titus Schlüter; Junko Kyozuka; Shingo Miyauchi; Ivo Feussner; Sabine Zachgo; Hirofumi Nakagami
The New phytologist, 243, 6, 2295, 2310, Sep. 2024, [International Magazine]
English, Scientific journal, The NPR proteins function as salicylic acid (SA) receptors in Arabidopsis thaliana. AtNPR1 plays a central role in SA-induced transcriptional reprogramming whereby positively regulates SA-mediated defense. NPRs are found in the genomes of nearly all land plants. However, we know little about the molecular functions and physiological roles of NPRs in most plant species. We conducted phylogenetic and alignment analyses of NPRs from 68 species covering the significant lineages of land plants. To investigate NPR functions in bryophyte lineages, we generated and characterized NPR loss-of-function mutants in the liverwort Marchantia polymorpha. Brassicaceae NPR1-like proteins have characteristically gained or lost functional residues identified in AtNPRs, pointing to the possibility of a unique evolutionary trajectory for the Brassicaceae NPR1-like proteins. We find that the only NPR in M. polymorpha, MpNPR, is not the master regulator of SA-induced transcriptional reprogramming and negatively regulates bacterial resistance in this species. The Mpnpr transcriptome suggested roles of MpNPR in heat and far-red light responses. We identify both Mpnpr and Atnpr1-1 display enhanced thermomorphogenesis. Interspecies complementation analysis indicated that the molecular properties of AtNPR1 and MpNPR are partially conserved. We further show that MpNPR has SA-binding activity. NPRs and NPR-associated pathways have evolved distinctively in diverged land plant lineages to cope with different terrestrial environments.

Cytokinin and ALOG proteins regulate pluripotent stem cell identity in the moss Physcomitrium patens.
Yuki Hata; Juri Ohtsuka; Yuji Hiwatashi; Satoshi Naramoto; Junko Kyozuka
Science advances, 10, 35, eadq6082, 30 Aug. 2024, [International Magazine]
English, Scientific journal, The shoot apical meristem (SAM) contains pluripotent stem cells that produce all the aerial parts of the plant. Stem cells undergo asymmetric cell divisions to self-renew and to produce differentiating cells. Our research focused on unraveling the mechanisms governing the specification of these two distinct cell fates following the stem cell division. For this purpose, we used the model organism Physcomitrium patens, which features a singular pluripotent stem cell known as the gametophore apical cell. We show that the activity of cytokinins, critical stem cell regulators, is restricted to the gametophore apical cell due to the specific localization of PpLOG, the enzyme responsible for cytokinin activation. In turn, PpTAW, which promotes differentiating cell identity of the merophyte, is excluded from the gametophore apical cell by the action of cytokinins. We propose a cytokinin-based model for the establishment of asymmetry in the pluripotent stem cell division.

Identification of a novel viral factor inducing tumorous symptoms by disturbing vascular development in planta.
Go Atsumi; Satoshi Naramoto; Masahiro Nishihara; Takashi Nakatsuka; Reiko Tomita; Yosuke Matsushita; Nobue Hoshi; Asuka Shirakawa; Kappei Kobayashi; Hiroo Fukuda; Ken-Taro Sekine
Journal of virology, e0046323, 05 Sep. 2023, [International Magazine]
English, Scientific journal, Plant viruses induce various disease symptoms that substantially impact agriculture, but the underlying mechanisms of viral disease in plants are poorly understood. Kobu-sho is a disease in gentian that shows gall formation with ectopic development of lignified cells and vascular tissues such as xylem. Here, we show that a gene fragment of gentian Kobu-sho-associated virus, which is designated as Kobu-sho-inducing factor (KOBU), induces gall formation accompanied by ectopic development of lignified cells and xylem-like tissue in Nicotiana benthamiana. Transgenic gentian expressing KOBU exhibited tumorous symptoms, confirming the gall-forming activity of KOBU. Surprisingly, KOBU expression can also induce differentiation of an additional leaf-like tissue on the abaxial side of veins in normal N. benthamiana and gentian leaves. Transcriptome analysis with Arabidopsis thaliana expressing KOBU revealed that KOBU activates signaling pathways that regulate xylem development. KOBU protein forms granules and plate-like structures and co-localizes with mRNA splicing factors within the nucleus. Our findings suggest that KOBU is a novel pleiotropic virulence factor that stimulates vascular and leaf development.IMPORTANCEWhile various mechanisms determine disease symptoms in plants depending on virus-host combinations, the details of how plant viruses induce symptoms remain largely unknown in most plant species. Kobu-sho is a disease in gentian that shows gall formation with ectopic development of lignified cells and vascular tissues such as xylem. Our findings demonstrate that a gene fragment of gentian Kobu-sho-associated virus (GKaV), which is designated as Kobu-sho-inducing factor, induces the gall formation accompanied by the ectopic development of lignified cells and xylem-like tissue in Nicotiana benthamiana. The molecular mechanism by which gentian Kobu-sho-associated virus induces the Kobu-sho symptoms will provide new insight into not only plant-virus interactions but also the regulatory mechanisms underlying vascular and leaf development.

Zinc homeostasis governed by Golgi-resident ZnT family members regulates ERp44-mediated proteostasis at the ER-Golgi interface.
Yuta Amagai; Momo Yamada; Toshiyuki Kowada; Tomomi Watanabe; Yuyin Du; Rong Liu; Satoshi Naramoto; Satoshi Watanabe; Junko Kyozuka; Tiziana Anelli; Tiziana Tempio; Roberto Sitia; Shin Mizukami; Kenji Inaba
Nature communications, 14, 1, 2683, 2683, 09 May 2023, [International Magazine]
English, Scientific journal, Many secretory enzymes acquire essential zinc ions (Zn2+) in the Golgi complex. ERp44, a chaperone operating in the early secretory pathway, also binds Zn2+ to regulate its client binding and release for the control of protein traffic and homeostasis. Notably, three membrane transporter complexes, ZnT4, ZnT5/ZnT6 and ZnT7, import Zn2+ into the Golgi lumen in exchange with protons. To identify their specific roles, we here perform quantitative Zn2+ imaging using super-resolution microscopy and Zn2+-probes targeted in specific Golgi subregions. Systematic ZnT-knockdowns reveal that ZnT4, ZnT5/ZnT6 and ZnT7 regulate labile Zn2+ concentration at the distal, medial, and proximal Golgi, respectively, consistent with their localization. Time-course imaging of cells undergoing synchronized secretory protein traffic and functional assays demonstrates that ZnT-mediated Zn2+ fluxes tune the localization, trafficking, and client-retrieval activity of ERp44. Altogether, this study provides deep mechanistic insights into how ZnTs control Zn2+ homeostasis and ERp44-mediated proteostasis along the early secretory pathway.

Control of vegetative reproduction in Marchantiapolymorpha by the KAI2-ligand signaling pathway.
Aino Komatsu; Kyoichi Kodama; Yohei Mizuno; Mizuki Fujibayashi; Satoshi Naramoto; Junko Kyozuka
Current biology : CB, 23 Feb. 2023, [International Magazine]
English, Scientific journal, In vegetative reproduction of Marchantia polymorpha (M. polymorpha), propagules, called gemmae, are formed in gemma cups. Despite its significance for survival, control of gemma and gemma cup formation by environmental cues is not well understood. We show here that the number of gemmae formed in a gemma cup is a genetic trait. Gemma formation starts from the central region of the floor of the gemma cup, proceeds to the periphery, and terminates when the appropriate number of gemmae is initiated. The MpKARRIKIN INSENSITIVE2 (MpKAI2)-dependent signaling pathway promotes gemma cup formation and gemma initiation. The number of gemmae in a cup is controlled by modulating the ON/OFF switch of the KAI2-dependent signaling. Termination of the signaling results in the accumulation of MpSMXL, a suppressor protein. In the Mpsmxl mutants, gemma initiation continues, leading to the formation of a highly increased number of gemmae in a cup. Consistent with its function, the MpKAI2-dependent signaling pathway is active in gemma cups where gemmae initiate, as well as in the notch region of the mature gemma and midrib of the ventral side of the thallus. In this work, we also show that GEMMA CUP-ASSOCIATED MYB1 works downstream of this signaling pathway to promote gemma cup formation and gemma initiation. We also found that the availability of potassium affects gemma cup formation independently from the KAI2-dependent signaling pathway in M. polymorpha. We propose that the KAI2-dependent signaling pathway functions to optimize vegetative reproduction by adapting to the environment in M. polymorpha.

The bryophytes Physcomitrium patens and Marchantia polymorpha as model systems for studying evolutionary cell and developmental biology in plants.
Satoshi Naramoto; Yuki Hata; Tomomichi Fujita; Junko Kyozuka
The Plant cell, 34, 1, 228, 246, 20 Jan. 2022, [International Magazine]
English, Scientific journal, Bryophytes are nonvascular spore-forming plants. Unlike in flowering plants, the gametophyte (haploid) generation of bryophytes dominates the sporophyte (diploid) generation. A comparison of bryophytes with flowering plants allows us to answer some fundamental questions raised in evolutionary cell and developmental biology. The moss Physcomitrium patens was the first bryophyte with a sequenced genome. Many cell and developmental studies have been conducted in this species using gene targeting by homologous recombination. The liverwort Marchantia polymorpha has recently emerged as an excellent model system with low genomic redundancy in most of its regulatory pathways. With the development of molecular genetic tools such as efficient genome editing, both P. patens and M. polymorpha have provided many valuable insights. Here, we review these advances with a special focus on polarity formation at the cell and tissue levels. We examine current knowledge regarding the cellular mechanisms of polarized cell elongation and cell division, including symmetric and asymmetric cell division. We also examine the role of polar auxin transport in mosses and liverworts. Finally, we discuss the future of evolutionary cell and developmental biological studies in plants.

NARROW AND DWARF LEAF 1, the Orthologue of Arabidopsis ENHANCER OF SHOOT REGENERATION1/DORNRÖSCHEN, Mediates Leaf Development and Maintenance of the Shoot Apical Meristem in Oryza sativa L.
Andree S Kusnandar; Jun-Ichi Itoh; Yutaka Sato; Eriko Honda; Ken-Ichiro Hibara; Junko Kyozuka; Satoshi Naramoto
Plant & cell physiology, 02 Dec. 2021, [Domestic magazines]
English, Scientific journal, The molecular basis for leaf development, a major focus in developmental biology, remains unclear in the monocotyledonous grass, rice (Oryza sativa). Here, we performed a mutant screen in rice and identified an AP2-type transcription factor family protein, NARROW AND DWARF LEAF1 (NDL1). NDL1 is the orthologue of Arabidopsis thaliana (subsequently called Arabidopsis) ENHANCER OF SHOOT REGENERATION1 (ESR1)/DORNRÖSCHEN (DRN) and mediates leaf development and maintenance of the shoot apical meristem (SAM). Loss of function of NDL1 results in bladeless leaves and SAMs that are flat, rather than dome-shaped, and lack cell proliferation activity. This loss of function also causes reduced auxin signaling. Moreover, as is the case with Arabidopsis ESR1/DRN, NDL1 plays crucial roles in shoot regeneration. Importantly, we found that NDL1 is not expressed in the SAM but is expressed in leaf primordia. We propose that NDL1 cell autonomously regulates leaf development, but non-cell autonomously regulates SAM maintenance in rice.

Major components of the KARRIKIN INSENSITIVE2-dependent signaling pathway are conserved in the liverwort Marchantia polymorpha.
Yohei Mizuno; Aino Komatsu; Shota Shimazaki; Satoshi Naramoto; Keisuke Inoue; Xiaonan Xie; Kimitsune Ishizaki; Takayuki Kohchi; Junko Kyozuka
The Plant cell, 33, 7, 2395, 2411, 11 Apr. 2021, [International Magazine]
English, Scientific journal, KARRIKIN INSENSITIVE2 (KAI2) was first identified as a receptor of karrikins, smoke-derived germination stimulants. KAI2 is also considered a receptor of an unidentified endogenous molecule called the KAI2-ligand (KL). Upon KAI2 activation, signals are transmitted through degradation of D53/SMXL proteins via MAX2-dependent ubiquitination. Although components in the KAI2-dependent signaling pathway, namely MpKAI2A and MpKAI2B, MpMAX2, and MpSMXL, exist in the genome of the liverwort Marchantia polymorpha, their functions remain unknown. Here, we show that early thallus growth is retarded and gemma dormancy in the dark is suppressed in Mpkai2a and Mpmax2 loss-of-function mutants. These defects are counteracted in Mpkai2a Mpsmxl and Mpmax2 Mpsmxl double mutants indicating that MpKAI2A, MpMAX2 and MpSMXL act in the same genetic pathway. Introduction of MpSMXLd53, in which a domain required for degradation is mutated, into wild-type plants mimicks Mpkai2a and Mpmax2 plants. In addition, detection of citrine fluorescence in Nicotiana benthamiana cells transiently expressing a SMXL-Citrine fusion protein requires treatment with MG132, a proteasome inhibitor. These findings imply that MpSMXL is subjected to degradation, and that degradation of MpSMXL is crucial for MpKAI2A-dependent signaling in M. polymorpha. Therefore, we claim that the basic mechanisms in the KAI2-dependent signaling pathway are conserved in M. polymorpha.

AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells.
Matouš Glanc; Kasper Van Gelderen; Lukas Hoermayer; Shutang Tan; Satoshi Naramoto; Xixi Zhang; David Domjan; Ludmila Včelařová; Robert Hauschild; Alexander Johnson; Edward de Koning; Maritza van Dop; Eike Rademacher; Stef Janson; Xiaoyu Wei; Gergely Molnár; Matyáš Fendrych; Bert De Rybel; Remko Offringa; Jiří Friml
Current biology : CB, 05 Mar. 2021, [International Magazine]
English, Scientific journal, Polar subcellular localization of the PIN exporters of the phytohormone auxin is a key determinant of directional, intercellular auxin transport and thus a central topic of both plant cell and developmental biology. Arabidopsis mutants lacking PID, a kinase that phosphorylates PINs, or the MAB4/MEL proteins of unknown molecular function display PIN polarity defects and phenocopy pin mutants, but mechanistic insights into how these factors convey PIN polarity are missing. Here, by combining protein biochemistry with quantitative live-cell imaging, we demonstrate that PINs, MAB4/MELs, and AGC kinases interact in the same complex at the plasma membrane. MAB4/MELs are recruited to the plasma membrane by the PINs and in concert with the AGC kinases maintain PIN polarity through limiting lateral diffusion-based escape of PINs from the polar domain. The PIN-MAB4/MEL-PID protein complex has self-reinforcing properties thanks to positive feedback between AGC kinase-mediated PIN phosphorylation and MAB4/MEL recruitment. We thus uncover the molecular mechanism by which AGC kinases and MAB4/MEL proteins regulate PIN localization and plant development.

Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana
Hongjiang Li; Daniel Wangenheim; Xixi Zhang; Shutang Tan; Nasser Darwish‐Miranda; Satoshi Naramoto; Krzysztof Wabnik; Riet De Rycke; Walter A. Kaufmann; Daniel Gütl; Ricardo Tejos; Peter Grones; Meiyu Ke; Xu Chen; Jan Dettmer; Jiří Friml
New Phytologist, Wiley, 18 Sep. 2020
Scientific journal

Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 106
Bussarin Wachananawat; Takeshi Kuroha; Yuto Takenaka; Hiroyuki Kajiura; Satoshi Naramoto; Ryusuke Yokoyama; Kimitsune Ishizaki; Kazuhiko Nishitani; Takeshi Ishimizu
Frontiers in Plant Science, 11, Frontiers Media SA, 02 Jul. 2020, [Peer-reviewed]
Scientific journal

Apical stem cells sustaining prosperous evolution of land plants.
Ryuichi Nishihama; Satoshi Naramoto
Journal of plant research, 133, 3, 279, 282, May 2020, [Invited], [Domestic magazines]
English

The origin and evolution of the ALOG proteins, members of a plant-specific transcription factor family, in land plants
Satoshi Naramoto; Yuki Hata; Junko Kyozuka
Journal of Plant Research, 133, 3, 323, 329, May 2020, [Peer-reviewed], [Invited], [Lead author, Corresponding author], [Domestic magazines]
English, Scientific journal, The Arabidopsis LSH1 and Oryza G1 (ALOG) protein is a family of plant-specific transcription factors that regulate reproductive growth in angiosperms. Despite their importance in plant development, little research has been conducted on ALOG proteins in basal land plants and the processes involved in their evolution remain largely unknown. Here, we studied the molecular evolution of ALOG family proteins. We found that ALOG proteins are absent in green algae but exist in all land plants analyzed as well as in some Charophycean algae, closest relatives of land plants. Multiple sequence alignments identified the high sequence conservation of ALOG domains in divergent plant lineages. Phylogenetic analyses also identified a distinct clade of ALOG protein member of lycophytes and bryophytes, including two of Marchantia polymorpha LATERAL ORGAN SUPPRESOR (MpLOS1 and MpLOS2) with a long branch length in MpLOS2. Consistent with this, the function of MpLOS1 was replaceable by Phycomitrella patens ALOG proteins, whereas MpLOS2 failed to replace the molecular function of MpLOS1. Moreover, the rice ALOG proteins, OsTAW1 and OsG1, were not able to replace the molecular function of MpLOS1 although we previously found that the function of OsG1 was replaceable by MpLOS1. Altogether, these findings suggest that ALOG proteins emerged before the evolution of land plants and that they exhibit functional conservation and diversification during the evolution of land plants. The finding that MpLOS1 is able to complement rice ALOG mutants but not vice versa also suggest the existence of conserved and the partly divergent functions of ALOG proteins in bryophytes and angiosperms.

A conserved regulatory mechanism mediates the convergent evolution of plant shoot lateral organs.
Satoshi Naramoto; Victor Arnold Shivas Jones; Nicola Trozzi; Mayuko Sato; Kiminori Toyooka; Masaki Shimamura; Sakiko Ishida; Kazuhiko Nishitani; Kimitsune Ishizaki; Ryuichi Nishihama; Takayuki Kohchi; Liam Dolan; Junko Kyozuka
PLoS biology, 17, 12, e3000560, Dec. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Land plant shoot structures evolved a diversity of lateral organs as morphological adaptations to the terrestrial environment, with lateral organs arising independently in different lineages. Vascular plants and bryophytes (basally diverging land plants) develop lateral organs from meristems of sporophytes and gametophytes, respectively. Understanding the mechanisms of lateral organ development among divergent plant lineages is crucial for understanding the evolutionary process of morphological diversification of land plants. However, our current knowledge of lateral organ differentiation mechanisms comes almost entirely from studies of seed plants, and thus, it remains unclear how these lateral structures evolved and whether common regulatory mechanisms control the development of analogous lateral organs. Here, we performed a mutant screen in the liverwort Marchantia polymorpha, a bryophyte, which produces gametophyte axes with nonphotosynthetic scalelike lateral organs. We found that an Arabidopsis LIGHT-DEPENDENT SHORT HYPOCOTYLS 1 and Oryza G1 (ALOG) family protein, named M. polymorpha LATERAL ORGAN SUPRESSOR 1 (MpLOS1), regulates meristem maintenance and lateral organ development in Marchantia. A mutation in MpLOS1, preferentially expressed in lateral organs, induces lateral organs with misspecified identity and increased cell number and, furthermore, causes defects in apical meristem maintenance. Remarkably, MpLOS1 expression rescued the elongated spikelet phenotype of a MpLOS1 homolog in rice. This suggests that ALOG genes regulate the development of lateral organs in both gametophyte and sporophyte shoots by repressing cell divisions. We propose that the recruitment of ALOG-mediated growth repression was in part responsible for the convergent evolution of independently evolved lateral organs among highly divergent plant lineages, contributing to the morphological diversification of land plants.

BLADE-ON-PETIOLE genes are not involved in the transition from protonema to gametophore in the moss Physcomitrella patens
Yuki Hata; Satoshi Naramoto; Junko Kyozuka
Journal of Plant Research, 132, 5, 617, 627, Springer Science and Business Media LLC, 02 Sep. 2019, [Domestic magazines]
English, Scientific journal, The timing of the transition between developmental phases is a critical determinant of plant form. In the moss Physcomitrella patens, the transition from protonema to gametophore is a particularly important step as it results in a change from two-dimensional to three-dimensional growth of the plant body. It is well known that this transition is promoted by cytokinin (CK), however, the underlying mechanisms are poorly understood. Previously, it was reported that P. patens orthologs of BLADE-ON-PETIOLE (BOP) genes (PpBOPs) work downstream of CK to promote the transition to gametophore. To further understand the role of PpBOPs in the control of this transition, we performed functional analyses of PpBOP genes. We simultaneously disrupted the function of all three PpBOP genes in P. patens using CRISPR technology, however, no abnormal phenotypes were observed in the triple mutant during either the gametophytic or the sporophytic growth stages. CK treatment did not alter the phase change in the triple mutant. We conclude that PpBOP genes are unnecessary in the control of P. patens development under normal conditions. We propose that BOP genes are not involved in the control of developmental processes in bryophytes and other basal land plants, but may function in physiological processes such as in the defense response.

Lateral organ diversification in plants mediated by the ALOG protein family of transcription factors
Naramoto S; Jones VAS; Trozzi N; Sato M; Toyooka K; Shimamura M; Ishida S; Nishitani K; Ishizaki K; Nishihama R; Kohchi T; Dolan L; Kyozuka J
Jul. 2019
Scientific journal

Cytokinin Signaling is Essential for Organ Formation in Marchantia polymorpha.
Aki SS; Mikami T; Naramoto S; Nishihama R; Ishizaki K; Kojima M; Takebayashi Y; Sakakibara H; Kyozuka J; Kohchi T; Umeda M
Plant Cell Physiol., May 2019, [Peer-reviewed]
English

BLADE-ON-PETIOLE genes temporally and developmentally regulate the sheath to blade ratio of rice leaves.
Toriba T; Tokunaga H; Shiga T; Nie F; Naramoto S; Honda E; Tanaka K; Taji T; Itoh JI; Kyozuka J
Nat Commun., 10, 1, 619, 619, Feb. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Axis formation is a fundamental issue in developmental biology. Axis formation and patterning in plant leaves is crucial for morphology and crop productivity. Here, we reveal the basis of proximal-distal patterning in rice leaves, which consist of a proximal sheath, a distal blade, and boundary organs formed between these two regions. Analysis of the three rice homologs of the Arabidopsis BLADE-ON-PETIOLE1 (BOP1) gene indicates that OsBOPs activate proximal sheath differentiation and suppress distal blade differentiation. Temporal expression changes of OsBOPs are responsible for the developmental changes in the sheath:blade ratio. We further identify that the change in the sheath:blade ratio during the juvenile phase is controlled by the miR156/SPL pathway, which modifies the level and pattern of expression of OsBOPs. OsBOPs are also essential for differentiation of the boundary organs. We propose that OsBOPs, the main regulators of proximal-distal patterning, control temporal changes in the sheath:blade ratio of rice leaves.

Gibberellin DELLA signaling targets the retromer complex to redirect protein trafficking to the plasma membrane
Yuliya Salanenka; Inge Verstraeten; Christian Löfke; Kaori Tabata K; Satoshi Naramoto; Matous Glanc; Jiří Friml
Proc Natl Acad Sci U S A, Feb. 2018, [Peer-reviewed]
English

ARF GTPase machinery at the plasma membrane regulates auxin transport-mediated plant growth
Satoshi Naramoto; Junko Kyozuka
Plant Biotechnology, 35, 2, 155, 159, 2018, [Peer-reviewed], [Invited], [Domestic magazines]
English, Scientific journal, VAN3 is a plant ACAP-type ADP-ribosylation factor-GTPase activating protein (ARF-GAP) that regulates auxin transport-mediated plant morphogenesis such as continuous venation and lateral root development in Arabidopsis. Previous studies suggested that VAN3 localizes at the plasma membrane (PM) and intracellular structures. However, the role of PM localization in mediating the van3 mutant phenotype is not clear. Here we performed subcellular localization analysis of VAN3 and its regulators CVP2 and VAB to determine their endogenous functions. We found that GFP-tagged CVP2 and VAB preferentially localize at the PM in stably transformed plants. We determined that transgenic plants with lower expression levels of GFP- or mRFP-tagged VAN3 displayed PM localization, which was sufficient to rescue the van3 mutant. Functional VAN3-mRFP and VAB-GFP colocalized at PMs. The van3 mutant phenotype was suppressed by mutation of VAN7/GNOM, which encodes an ARF-GEF that localizes at the PM and Golgi apparatus. These combined results suggest that ARF-GTPase machinery at the PM regulates auxin transport-mediated plant growth and development.

Deletion analysis of AGD1 reveals domains crucial for plasma membrane recruitment and function in root hair polarity
Cheol-Min Yoo; Satoshi Naramoto; J. Alan Sparks; Bibi Rafeiza Khan; Jin Nakashima; Hiroo Fukuda; Elison B. Blancaflor
Journal of Cell Science, 131, 2, Company of Biologists Ltd, 2018, [Peer-reviewed]
English, Scientific journal

Polar transport in plants mediated by membrane transporters: focus on mechanisms of polar auxin transport
Satoshi Naramoto
CURRENT OPINION IN PLANT BIOLOGY, 40, 8, 14, Dec. 2017, [Peer-reviewed], [Invited]
English, Scientific journal

Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome.
John L Bowman; Takayuki Kohchi; Katsuyuki T Yamato; Jerry Jenkins; Shengqiang Shu; Kimitsune Ishizaki; Shohei Yamaoka; Ryuichi Nishihama; Yasukazu Nakamura; Frédéric Berger; Catherine Adam; Shiori Sugamata Aki; Felix Althoff; Takashi Araki; Mario A Arteaga-Vazquez; Sureshkumar Balasubrmanian; Kerrie Barry; Diane Bauer; Christian R Boehm; Liam Briginshaw; Juan Caballero-Perez; Bruno Catarino; Feng Chen; Shota Chiyoda; Mansi Chovatia; Kevin M Davies; Mihails Delmans; Taku Demura; Tom Dierschke; Liam Dolan; Ana E Dorantes-Acosta; D Magnus Eklund; Stevie N Florent; Eduardo Flores-Sandoval; Asao Fujiyama; Hideya Fukuzawa; Bence Galik; Daniel Grimanelli; Jane Grimwood; Ueli Grossniklaus; Takahiro Hamada; Jim Haseloff; Alexander J Hetherington; Asuka Higo; Yuki Hirakawa; Hope N Hundley; Yoko Ikeda; Keisuke Inoue; Shin-Ichiro Inoue; Sakiko Ishida; Qidong Jia; Mitsuru Kakita; Takehiko Kanazawa; Yosuke Kawai; Tomokazu Kawashima; Megan Kennedy; Keita Kinose; Toshinori Kinoshita; Yuji Kohara; Eri Koide; Kenji Komatsu; Sarah Kopischke; Minoru Kubo; Junko Kyozuka; Ulf Lagercrantz; Shih-Shun Lin; Erika Lindquist; Anna M Lipzen; Chia-Wei Lu; Efraín De Luna; Robert A Martienssen; Naoki Minamino; Masaharu Mizutani; Miya Mizutani; Nobuyoshi Mochizuki; Isabel Monte; Rebecca Mosher; Hideki Nagasaki; Hirofumi Nakagami; Satoshi Naramoto; Kazuhiko Nishitani; Misato Ohtani; Takashi Okamoto; Masaki Okumura; Jeremy Phillips; Bernardo Pollak; Anke Reinders; Moritz Rövekamp; Ryosuke Sano; Shinichiro Sawa; Marc W Schmid; Makoto Shirakawa; Roberto Solano; Alexander Spunde; Noriyuki Suetsugu; Sumio Sugano; Akifumi Sugiyama; Rui Sun; Yutaka Suzuki; Mizuki Takenaka; Daisuke Takezawa; Hirokazu Tomogane; Masayuki Tsuzuki; Takashi Ueda; Masaaki Umeda; John M Ward; Yuichiro Watanabe; Kazufumi Yazaki; Ryusuke Yokoyama; Yoshihiro Yoshitake; Izumi Yotsui; Sabine Zachgo; Jeremy Schmutz
Cell, 171, 2, 287, 304, 05 Oct. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal, The evolution of land flora transformed the terrestrial environment. Land plants evolved from an ancestral charophycean alga from which they inherited developmental, biochemical, and cell biological attributes. Additional biochemical and physiological adaptations to land, and a life cycle with an alternation between multicellular haploid and diploid generations that facilitated efficient dispersal of desiccation tolerant spores, evolved in the ancestral land plant. We analyzed the genome of the liverwort Marchantia polymorpha, a member of a basal land plant lineage. Relative to charophycean algae, land plant genomes are characterized by genes encoding novel biochemical pathways, new phytohormone signaling pathways (notably auxin), expanded repertoires of signaling pathways, and increased diversity in some transcription factor families. Compared with other sequenced land plants, M. polymorpha exhibits low genetic redundancy in most regulatory pathways, with this portion of its genome resembling that predicted for the ancestral land plant. PAPERCLIP.

Cellular and developmental function of ACAP type ARF-GAP proteins are diverged in plant cells
Satoshi Naramoto; Tomoko Dainobu; Hiroki Tokunaga; Junko Kyozuka; Hiroo Fukuda
PLANT BIOTECHNOLOGY, 33, 4, 309, +, Sep. 2016, [Peer-reviewed]
English, Scientific journal

Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells
Lukasz Langowski; Krzysztof Wabnik; Hongjiang Li; Steffen Vanneste; Satoshi Naramoto; Hirokazu Tanaka; Jiri Friml
CELL DISCOVERY, 2, Jul. 2016, [Peer-reviewed]
English, Scientific journal

A bioimaging pipeline to show membrane trafficking regulators localized to Golgi apparatus and other organelles in plant cells
Satoshi Naramoto; Tomoko Dainobu; Marisa Otegui
Bioprotocol, 5, 17, 1583, 05 Sep. 2015, [Peer-reviewed], [Invited]
English, Scientific journal

Insights into the Localization and Function of the Membrane Trafficking Regulator GNOM ARF-GEF at the Golgi Apparatus in Arabidopsis
Satoshi Naramoto; Marisa S. Otegui; Natsumaro Kutsuna; Riet de Rycke; Tomoko Dainobu; Michael Karampelias; Masaru Fujimoto; Elena Feraru; Daisuke Miki; Hiroo Fukuda; Akihiko Nakano; Jiri Friml
PLANT CELL, 26, 7, 3062, 3076, Jul. 2014, [Peer-reviewed]
English, Scientific journal

VAN4 Encodes a Putative TRS120 That is Required for Normal Cell Growth and Vein Development in Arabidopsis
Satoshi Naramoto; Tomasz Nodzynski; Tomoko Dainobu; Hirotomo Takatsuka; Teruyo Okada; Jiri Friml; Hiroo Fukuda
PLANT AND CELL PHYSIOLOGY, 55, 4, 750, 763, Apr. 2014, [Peer-reviewed], [Invited]
English, Scientific journal

Increase in vascular pattern complexity caused by mutations in LHY and CCA1 in Arabidopsis thaliana under continuous light
Kohei Aihara; Satoshi Naramoto; Miyuki Hara; Tsuyoshi Mizoguchi
PLANT BIOTECHNOLOGY, 31, 1, 43, +, 2014, [Peer-reviewed]
English, Scientific journal

RAB5 activation is required for multiple steps in arabidopsis thaliana root development
Takeshi Inoue; Yuki Kondo; Satoshi Naramoto; Akihiko Nakano; Takashi Ueda
Plant and Cell Physiology, 54, 10, 1648, 1659, Oct. 2013, [Peer-reviewed]
English, Scientific journal

ABP1 and ROP6 GTPase Signaling Regulate Clathrin-Mediated Endocytosis in Arabidopsis Roots
Xu Chen; Satoshi Naramoto; Stephanie Robert; Ricardo Tejos; Christian Loefke; Deshu Lin; Zhenbiao Yang; Jiri Friml
CURRENT BIOLOGY, 22, 14, 1326, 1332, Jul. 2012, [Peer-reviewed]
English, Scientific journal

Auxin-Dependent Cell Cycle Reactivation through Transcriptional Regulation of Arabidopsis E2Fa by Lateral Organ Boundary Proteins
Barbara Berckmans; Valya Vassileva; Stephan P. C. Schmid; Sara Maes; Boris Parizot; Satoshi Naramoto; Zoltan Magyar; Claire Lessa Alvim Kamei; Csaba Koncz; Laszlo Boegre; Geert Persiau; Geert De Jaeger; Jiri Friml; Ruediger Simon; Tom Beeckman; Lieven De Veylder
PLANT CELL, 23, 10, 3671, 3683, Oct. 2011, [Peer-reviewed]
English, Scientific journal

Recycling, clustering, and endocytosis jointly maintain PIN auxin carrier polarity at the plasma membrane
Juergen Kleine-Vehn; Krzysztof Wabnik; Alexandre Martiniere; Lukasz Langowski; Katrin Willig; Satoshi Naramoto; Johannes Leitner; Hirokazu Tanaka; Stefan Jakobs; Stephanie Robert; Christian Luschnig; Willy Govaerts; Stefan W. Hell; John Runions; Jiri Friml
MOLECULAR SYSTEMS BIOLOGY, 7, 540, Oct. 2011, [Peer-reviewed]
English, Scientific journal

Cell Plate Restricted Association of DRP1A and PIN Proteins Is Required for Cell Polarity Establishment in Arabidopsis
Jozef Mravec; Jan Petrasek; Na Li; Sjef Boeren; Rumyana Karlova; Saeko Kitakura; Marketa Parezova; Satoshi Naramoto; Tomasz Nodzynski; Pankaj Dhonukshe; Sebastian Y. Bednarek; Eva Zazimalova; Sacco de Vries; Jiri Friml
CURRENT BIOLOGY, 21, 12, 1055, 1060, Jun. 2011, [Peer-reviewed]
English, Scientific journal

ARF1 Localizes to the Golgi and the Trans-Golgi Network
David G. Robinson; David Scheuring; Satoshi Naramoto; Jiri Friml
PLANT CELL, 23, 3, 846, 849, Mar. 2011, [Peer-reviewed]
English

ADP-ribosylation factor machinery mediates endocytosis in plant cells
Satoshi Naramoto; Jurgen Kleine-Vehn; Stephanie Robert; Masaru Fujimoto; Tomoko Dainobu; Tomasz Paciorek; Takashi Ueda; Akihiko Nakano; Marc C. E. Van Montagu; Hiroo Fukuda; Jiri Friml
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107, 50, 21890, 21895, Dec. 2010, [Peer-reviewed]
English, Scientific journal

Emergence of tissue polarization from synergy of intracellular and extracellular auxin signaling
Krzysztof Wabnik; Juergen Kleine-Vehn; Jozef Balla; Michael Sauer; Satoshi Naramoto; Vilem Reinoehl; Roeland M. H. Merks; Willy Govaerts; Jiri Friml
MOLECULAR SYSTEMS BIOLOGY, 6, 447, Dec. 2010, [Peer-reviewed]
English, Scientific journal

Trafficking to the Outer Polar Domain Defines the Root-Soil Interface
Lukasz Langowski; Kamil Ruzicka; Satoshi Naramoto; Juergen Kleine-Vehn; Jiri Friml
CURRENT BIOLOGY, 20, 10, 904, 908, May 2010, [Peer-reviewed]
English, Scientific journal

PIN Auxin Efflux Carrier Polarity Is Regulated by PINOID Kinase-Mediated Recruitment into GNOM-Independent Trafficking in Arabidopsis
Juergen Kleine-Vehn; Fang Huang; Satoshi Naramoto; Jing Zhang; Marta Michniewicz; Remko Offringa; Jiri Friml
PLANT CELL, 21, 12, 3839, 3849, Dec. 2009, [Peer-reviewed]
English, Scientific journal

Phosphoinositide-dependent regulation of VAN3 ARF-GAP localization and activity essential for vascular tissue continuity in plants
Satoshi Naramoto; Shinichiro Sawa; Koji Koizumi; Tomohiro Uemura; Takashi Ueda; Jiri Friml; Akihiko Nakano; Hiroo Fukuda
DEVELOPMENT, 136, 9, 1529, 1538, May 2009, [Peer-reviewed]
English, Scientific journal

The PIN-FORMED (PIN) protein family of auxin transporters.
Krecek P; Skupa P; Libus J; Naramoto S; Tejos R; Friml J; Zazimalova E
Genome biology, 10, 12, 249, 2009, [Peer-reviewed]

[Elaborate vesicle transport system behind the formation of plant cell polarity: focusing on the auxin mediated plant development].
Naramoto S
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 53, 16 Suppl, 2320, 2325, 共立出版, Dec. 2008, [Peer-reviewed]
Japanese

DRP1A is responsible for vascular continuity synergistically working with VAN3 in Arabidopsis
S Sawa; K Koizumi; S Naramoto; T Demura; T Ueda; A Nakano; H Fukuda
PLANT PHYSIOLOGY, 138, 2, 819, 826, Jun. 2005, [Peer-reviewed]
English, Scientific journal

VAN3 ARF-GAP-mediated vesicle transport is involved in leaf vascular network formation
K Koizumi; S Naramoto; S Sawa; N Yahara; T Ueda; A Nakano; M Sugiyama; H Fukuda
DEVELOPMENT, 132, 7, 1699, 1711, Apr. 2005, [Peer-reviewed]
English, Scientific journal

植物の体軸形成の分子基盤
楢本 悟史, アグリバイオ, 3, 4, 49, 51, Apr. 2019, [Invited]
Japanese, Introduction commerce magazine

ゼニゴケにおけるEndoglucanase16の機能解析
石田光南; 黒羽剛; 石崎公庸; 檜垣匠; 楢本悟史; 横山隆亮; 小竹敬久; 西谷和彦, 日本植物生理学会年会(Web), 60th, 2019

KAI2シグナル伝達経路はゼニゴケの形態形成を制御する
水野陽平; 楢本悟史; 小松愛乃; 島崎翔太; 児玉恭一; 石崎公庸; 経塚淳子, 日本植物学会, Sep. 2018
Japanese, Summary national conference

分野別必読論文選考対談
Satosi Naramoto, 細胞工学, 35, 3, 190, 193, 21 Apr. 2016
秀潤社, Japanese, Introduction other

最適な繁殖のためのメリステム無限成長性の制御
経塚淳子; 徳永浩樹; 楢本悟史; 鳥羽大陽; 吉田明希子, 日本植物学会大会研究発表記録, 80th, 2016

メリステム相転換を制御するイネTAWAWA1遺伝子に関する進化発生生物学的解析
楢本悟史; 徳永浩樹; 塚本成幸; 高見英幸; 嶋村正樹; 吉田明希子; 石崎公庸; 西浜竜一; 河内孝之; 経塚淳子, 日本分子生物学会年会プログラム・要旨集(Web), 39th, ROMBUNNO.2PS12‐5(2P‐0454) (WEB ONLY), 2016
Japanese

バイオ論文解説総集偏1
Satoshi Naramoto, 細胞工学, Sep. 2015
秀潤社, Japanese, Introduction other

ABP1 and ROP6 GTPase Signaling Regulate Clathrin-Mediated Endocytosis in Arabidopsis Roots (vol 22, pg 1326, 2012)
Xu Chen; Satoshi Naramoto; Stephanie Robert; Ricardo Tejos; Christian Lofke; Deshu Lin; Zhenbiao Yang; Jiri Friml, CURRENT BIOLOGY, 22, 14, 1358, 1358, Jul. 2012
English, Others

The PIN-FORMED (PIN) protein family of auxin transporters
Pavel Krecek; Petr Skupa; Jiri Libus; Satoshi Naramoto; Ricardo Tejos; Jiri Friml; Eva Zazimalova, GENOME BIOLOGY, 10, 12, 2009
English, Book review

植物におけるメンブレントラフィック 小胞輸送から考える植物細胞の極性形成機構 オーキシンを中心として
楢本悟史, 蛋白質 核酸 酵素, 53, 16, 2320, 2325, Dec. 2008, [Invited]
Japanese

Analysis of phosphoinositide signaling mediating vascular continuity
Satoshi Naramoto; Shinichiro Sawa; Koji Koizumi; Takashi Ueda; Akihiko Nakano; Hiroo Fukuda, PLANT AND CELL PHYSIOLOGY, 48, S53, S53, 2007
English, Summary international conference

Cell biological analysis of the van3 mutant of Arabidopsis involved in vascular patterning
NARAMOTO Satoshi; SAWA Schinichiro; KOIZUMI Koji; UEDA Takashi; NAKANO Akihiko; FUKUDA Hiroo, Journal of plant research, 119, 149, 149, 01 Dec. 2006
English

Gene expression at a cellular level and observation method of protein (2-2). Whole mount fluorescent antibody technique.
楢本悟史, 細胞工学, 106-110,14, Apr. 2006, [Invited]
Japanese

Intracellular and intercellular transport mechanisms of xylogen
H Motose; S Naramoto; Y Watanabe; M Sugiyama; H Fukuda, PLANT AND CELL PHYSIOLOGY, 47, S196, S196, 2006
English, Summary international conference

Genetic Analysis of VAN3 and its homologous genes involved in vascular patterning.
S Naramoto; S Sawa; K Koizumi; M Kubo; T Demura; T Ueda; A Nakano; H Fukuda, PLANT AND CELL PHYSIOLOGY, 46, S44, S44, 2005
English, Summary international conference

Cloning of the arabidopsis VAN3 gene involved in leaf vein patterning
K Koizumi; S Naramoto; S Sawa; M Sugiyama; H Fukuda, PLANT AND CELL PHYSIOLOGY, 45, S164, S164, 2004
English, Summary international conference

ADL1A, an VAN3 interacting protein, regulates single cell differentiation in Arabidopsis.
S Sawa; K Koizumi; S Naramoto; H Fukuda, PLANT AND CELL PHYSIOLOGY, 45, S165, S165, 2004
English, Summary international conference

VAN3, an Arabidopsis novel type ArfGAP protein, functions in a vesicle transport governing vascular continuity
S Naramoto; S Sawa; K Koizumi; Kurihara, I; M Kubo; T Demura; N Yahara; T Ueda; A Nakano; H Fukuda, PLANT AND CELL PHYSIOLOGY, 45, S165, S165, 2004
English, Summary international conference

Characterization of the vascular-network defective mutant, van3, of arabidopsis
K Koizumi; S Naramoto; S Sawa; Y Kuno; S Tanaka; M Sugiyama; H Fukuda, PLANT AND CELL PHYSIOLOGY, 44, S106, S106, 2003
English, Summary international conference

PpTAWs, encoding an ALOG family transcription factor, is required for stem cell maintenance in Physcomitrella patens
NARAMOTO Satoshi
日本植物生理学会第60回大会, 13 Mar. 2019, English, Poster presentation
[Domestic Conference]

lateral organ diversification in plants mediated by an ALOG family protein
NARAMOTO Satoshi
東北植物学会第8回大会, 08 Dec. 2018, Japanese, Oral presentation
[Domestic Conference]

Studies on molecular mechanisms of micro domain formation at plasma membranes that govern polar localization of auxin efflux carrier PIN proteins
NARAMOTO Satoshi
日本分子生物学会第41回大会, 28 Nov. 2018, English, Poster presentation
[Invited], [Domestic Conference]

Coordination of lateral organ development and meristem activity mediated by ALOG protein in Marchantia polymorpha
Satoshi Naramoto; Kimitsune Ishizaki; Masaki Shimamura; Sakiko Ishida; Ryuichi Nishihama; Takayuki Kohchi; Junko Kyozuka
植物学会, 14 Sep. 2018, English, Public symposium
[Invited], [Domestic Conference]

Coordination of lateral organ development and stem cell activity in Marchantia polymorpha is mediated by an ALOG family protein
Satoshi Naramoto; Nicola Trozzi; Victor Jones; Masaki Shimamura; Kanane Sato; Sakiko Ishida; Kimitsune Ishizaki; Ryuichi Nishihama; Takayuki Kohchi; Junko Kyozuka
EMBO workshop New shores in land plant evolution, 21 Jun. 2018, English, Poster presentation
[International presentation]

植物細胞の極性形成機構の細胞生物学的解析
NARAMOTO Satoshi
第20回オルガネラワークショップ, 27 Mar. 2018, Japanese, Keynote oral presentation
[Invited], [Domestic Conference]

側性器官は頂端分裂組織を制御するのか？
NARAMOTO Satoshi
第1回コケ幹細胞研究会, 06 Jan. 2018, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

Evolutionary-Developmental Analysis of ALOG Family Protein in Marchantia polymorpha
Satoshi Naramoto; Kimitsune Ishizaki; Masaki Shimamura; Sakiko Ishida; Ryuichi Nishihama; Takayuki Kohchi; Junko Kyozuka
The 85th NIBB Conference Marchanita Workshop, 17 Dec. 2017, English, Invited oral presentation
[Invited], [International presentation]

植物の極性構築メカニズムの分子細胞生物学的研究
NARAMOTO Satoshi
東北植物学会, 10 Dec. 2017, Japanese, Invited oral presentation
[Invited], [Domestic Conference]

ゼニゴケALOGドメイン遺伝子MpTAW1の進化発生学的解析
楢本悟史; 石崎公庸; 嶋村正樹; 徳永浩樹; 吉田明希子; 西浜竜一; 河内孝之; 経塚淳子
植物学会第81回大会, 09 Sep. 2017, Japanese, Oral presentation
[Domestic Conference]

Molecular and cell biological studies on the mechanisms of plant axis formation
NARAMOTO Satoshi
第35回日本植物細胞分子生物学会, 30 Aug. 2017, Keynote oral presentation
[Invited], [Domestic Conference]

Evolutionary-developmental analysis of ALOG family protein in Marchantia polymorpha
楢本悟史; 石崎公庸; 嶋村正樹; 徳永浩樹; 吉田明希子; 西浜竜一; 河内孝之; 経塚淳子
第35回日本植物細胞分子生物学会, 29 Aug. 2017, Japanese, Oral presentation
[Domestic Conference]

小胞輸送制御因子とオーキシン排出担体PINによる植物細胞の極性形成機構
楢本悟史; 古谷将彦; 中野明彦; 福田裕穂; 経塚淳子
第69回細胞生物学会大会, 15 Jun. 2017, Japanese, Invited oral presentation
[Domestic Conference]

ゼニゴケALOGドメイン遺伝子MpTAW1の機能解析
楢本悟史; 石崎公庸; 嶋村正樹; 石田咲子; 徳永浩樹; 吉田明希子; 西浜竜一; 河内孝之; 経塚淳子
第58回日本植物生理学会年会, 17 Mar. 2017, Japanese, Oral presentation
[Domestic Conference]

メリステム相転換を制御するイネTAWAWA1遺伝子に関する 進化発生生物学的解析
第39回日本分子生物学会年会, 30 Nov. 2016, Public symposium
[Domestic Conference]

ゼニゴケALOGドメイン遺伝子MpTAW1の機能解析
第57回日本植物生理学会年会, 18 Mar. 2016, Poster presentation
[Domestic Conference]

小胞輸送制御因子とオーキシン排出担体PINによる植物細胞の極性形成機構
東北植物学会第5回大会, 19 Dec. 2015, Poster presentation
[Domestic Conference]

小胞輸送制御因子とオーキシン排出担体PINによる植物細胞の極性形成機構
第10回東北育種研究集会, 14 Nov. 2015, Poster presentation
[Domestic Conference]

リンドウこぶ症関連ウイルスのGK32遺伝子断片の発現は,シロイヌナズナにおいて木部の分化を制御するシグナル伝達系を活性化する
厚見剛; 楢本悟史; 冨田麗子; 白川明日佳; 関根健太郎
日本植物病理学会大会プログラム・講演要旨予稿集, 10 Mar. 2015, Japanese

Insights into the Localization and Function of the Membrane Trafficking Regulator GNOM ARF-GEF at the Golgi Apparatus in Arabidopsis
NARAMOTO Satoshi; OTEGUI Marisa; DE RYCKE Riet; DAINOBU Tomoko; EUKUDA Hiroo; NAKANO Akihiko; FRIML Jiri
日本植物生理学会年会要旨集, 09 Mar. 2015, English

Role of phosphorylation of cellulose synthase during secondary cell wall development
OKADA Teruyo; NARAMOTO Satoshi; ENDO Satoshi; SAITO Chieko; FUKUDA Hiroo
日本植物生理学会年会要旨集, 09 Mar. 2015, English

二次細胞壁形成で発現する遺伝子の網羅的機能解析
遠藤暁詩; 楢本悟史; 齊藤知恵子; 福田裕穂
日本植物生理学会年会要旨集, 09 Mar. 2015, Japanese

Insights into the localization and function of GNOM ARF-GEF at the Golgi apparatus in arabidopsis
NARAMOTO Satoshi; OTEGUI Marisa; RYCKE Riet; DAISHIN Tomoko; FUKUDA Hiroo; NAKANO Akihiko; FRIML Jiri
日本植物学会大会研究発表記録, 01 Sep. 2014, English

液胞膜の形態に異常を示す変異体の単離と表現型解析
齊藤知恵子; 楢本悟史; 遠藤暁詩; 坂本智昭; 倉田哲也; 中野明彦; 福田裕穂
日本植物学会大会研究発表記録, 01 Sep. 2014, Japanese

木質バイオマス改変技術の開発を目指した二次細胞壁形成関連遺伝子の解析
大槻和弘; 楢本悟史; 遠藤暁詩; 福田裕穂
日本植物学会大会研究発表記録, 01 Sep. 2014, Japanese

VAN4 encodes a putative TRS120 that is involved required for normal cell growth and vein development in Arabidopsis
第55 回日本植物生理学会年会, 18 Mar. 2014, Oral presentation
[Domestic Conference]

二次細胞壁形成で発現する遺伝子の網羅的機能解析
遠藤暁詩; 楢本悟史; 齊藤知恵子; 福田裕穂
日本植物生理学会年会要旨集, 11 Mar. 2014, Japanese

細胞膜タンパク質の極性局在様式とドメイン構造
楢本悟史; LANGOWSKI Lukasz; FRIML Jiri; 中野明彦; 福田裕穂
日本植物学会大会研究発表記録, 20 Aug. 2013, Japanese

液胞上の複雑な膜構造の形態に異常を示す変異体の単離と表現型解析
齊藤知恵子; 楢本悟史; 遠藤暁詩; 坂本智昭; 倉田哲也; 上田貴志; 中野明彦; 福田裕穂
日本植物学会大会研究発表記録, 20 Aug. 2013, Japanese

二次細胞壁形成で発現する100遺伝子の機能解析
遠藤暁詩; 楢本悟史; 齊藤知恵子; 福田裕穂
日本植物生理学会年会要旨集, 14 Mar. 2013, Japanese

液胞とbulbの形態に異常をきたす変異体候補#64の原因遺伝子同定と解析
齊藤知恵子; 木内玲子; 中野雄司; 楢本悟史; 植村知博; 粟井千絵; 山上あゆみ; 坂本智昭; 倉田哲也; 安部弘; 上田貴志; 中野明彦
日本植物学会大会研究発表記録, 14 Sep. 2012, Japanese

細胞極性を司るARF GTPase制御因子の解析
楢本悟史; JURGEN Kleine‐Vehn; STEPHANIE Robert; 藤本優; 台信友子; 上田貴志; 中野明彦; 福田裕穂; JIRI Friml
日本植物生理学会年会要旨集, 09 Mar. 2012, Japanese

細胞極性制御に関わるARF GTPase regulatorの解析
楢本悟史; JUERGEN Kleine‐Vehn; STEPHANIE Robert; 藤本優; 台信友子; TOMASZ Paciorek; 上田貴志; 中野明彦; 福田裕穂; JIRI Friml
日本植物学会大会研究発表記録, 10 Sep. 2011, Japanese

脂質性シグナル伝達系を介した維管束の連続性構築機構の解析
楢本悟史; 澤進一郎; 小泉好司; 上田貴志; 中野明彦; 福田裕穂
日本植物生理学会年会要旨集, 15 Mar. 2007, Japanese

Xylogenの細胞内・細胞間輸送機構の解析
本瀬宏康; 楢本悟史; 渡辺雄一郎; 杉山宗隆; 福田裕穂
日本植物生理学会年会要旨集, Mar. 2006, Japanese

シロイヌナズナvan突然変異体を用いた維管束パターン形成機構の解析
楢本悟史; 沢進一郎; 小泉好司; 上田貴志; 中野明彦; 福田裕穂
日本分子生物学会年会講演要旨集, 25 Nov. 2005, Japanese

葉脈形成に関与するシロイヌナズナVAN3遺伝子及びそのホモログの分子遺伝学的解析
楢本悟史; 沢進一郎; 小泉好司; 久保稔; 出村拓; 上田貴志; 中野明彦; 福田裕穂
日本植物生理学会年会要旨集, Mar. 2005, Japanese

葉脈形成に関わるシロイヌナズナVAN3遺伝子のクローニング
小泉好司; 楢本悟史; 沢進一郎; 杉山宗隆; 福田裕穂
日本植物生理学会年会要旨集, 20 Mar. 2004, Japanese

葉脈形成に関わるシロイヌナズナVAN3タンパク質と相互作用する因子の単離・解析
沢進一郎; 小泉好司; 楢本悟史; 福田裕穂
日本植物生理学会年会要旨集, 20 Mar. 2004, Japanese

シロイヌナズナVAN3遺伝子は葉脈の連続性を構築する小胞輸送機構に働く
楢本悟史; 沢進一郎; 小泉好司; 栗原逸平; 久保稔; 出村拓; 矢原奈津子; 上田貴史; 中野明彦
日本植物生理学会年会要旨集, 20 Mar. 2004, Japanese

シロイヌナズナの葉脈不連続突然変異体van4の解析
楢本悟史; 小泉好司; 岩本訓知; 杉山宗隆; 福田裕穂
日本植物学会大会研究発表記録, 20 Sep. 2002, Japanese

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生物学Ⅰ, 2024年, 学士課程, 全学教育

ISP生物科学実習Ⅱ・a, 2024年, 学士課程, 理学部

ISP生物科学実習Ⅱ・b, 2024年, 学士課程, 理学部

形態機能学実習, 2024年, 学士課程, 理学部

形態機能学実習, 2024年, 学士課程, 理学部

形態機能学Ⅱ, 2024年, 学士課程, 理学部

形態機能学b, 2024年, 学士課程, 理学部

形態機能学Ⅱ, 2024年, 学士課程, 理学部

生物学特別講義Ⅱ, 2024年, 学士課程, 理学部

生物学特別講義Ⅱ, 2024年, 学士課程, 理学部

生物学Ⅰ, 2024年, 学士課程, 全学教育

細胞生物学Ⅲ, 2024年, 学士課程, 理学部

基礎形態学実習, 2024年, 学士課程, 理学部

基礎形態学実習, 2024年, 学士課程, 理学部

緩やかな細胞極性が組織・器官を形成するしくみの解析
科学研究費助成事業
01 Apr. 2024 - 31 Mar. 2027
楢本 悟史
日本学術振興会, 学術変革領域研究(B), 北海道大学, 24H00856

多細胞生物の柔軟な形態を支えるスピングラス的発生システム
科学研究費助成事業
01 Apr. 2024 - 31 Mar. 2027
北沢 美帆; 木下 温子; 楢本 悟史; 西村 岳志
日本学術振興会, 学術変革領域研究(B), 大阪大学, 24H00854

オーキシン極性輸送をモデルとした体軸の形成・維持機構の解明
2022 - 2025
楢本 悟史
オーキシンの極性輸送は、植物の形態形成を制御する重要な現象ですが、その輸送方向はオーキシン排出担体PINの細胞における偏在化で規定されます。本研究では、PINが細胞膜上で形成する新奇複合体様構造に注目した研究を行うことで、従来のモデルに代わる新しい細胞極性・体軸形成モデルを提案します。本研究は、オーキシンをはじめとした植物分子の輸送方向をデザインする基盤技術の創出に繋がる可能性があります。
科学技術振興機構, 戦略的な研究開発の推進/戦略的創造研究推進事業/さきがけ, 22683627

細胞極性の自律周期的形成とその変調が導出するオーキシン極性輸送システム
科学研究費助成事業
01 Apr. 2022 - 31 Mar. 2024
楢本 悟史
日本学術振興会, 新学術領域研究(研究領域提案型), 北海道大学, 22H04708

オーキシン極性輸送の成立に不可欠な新現象「PINクラスター」形成に関する研究
科学研究費助成事業
01 Apr. 2020 - 31 Mar. 2023
楢本 悟史
申請者は、 オーキシン排出トランスポーターであるPINタンパク質が細胞膜上で多量体様のクラスター構造を形成すること（以下、PINクラスター）、ならびに、PINクラスターが微小管とは排他的に局在することを独自に見出した。本研究においては、シロイヌナズナにおけるPINクラスターの生理機能の解明を目指す。また、ゼニゴケなどの他植物種におけるPINクラスターの保存性の有無について解析を行う。
本年度は、昨年度作成したPIN2とMAB4の蛍光タンパク質の共発現体を用いた観察を行った。共焦点レーザー顕微鏡を用いて、PIN2とMAB4の同時観察を行うことで、PIN2とMAB4は高頻度に共局在しており、両者は直接相互作用することを見出した。また、PINは、細胞分裂直後には細胞板ではクラスターを形成していないことを明らかにした。加えて、PINは、生物学的相分離の様式を経てクラスター形成されることを示唆する結果を得た。
一方、ゼニゴケにおいては、昨年度作成したPIN1-GFP発現体の観察を行った。その結果、予備的な実験ながらも、PIN1は細胞の腹側の部位に局在する可能性が示唆された。また、シロイヌナズナにおけるPINの局在性御に関わる因子の突然変異体を作成することで、これらは、Mppin1変異体と類似の表現型を示すことを見出した。本結果により、ゼニゴケはシロイヌナズナとは異なり、明確な形態形成を行わないにも関わらず、PINの局在制御に関わる分子メカニズムが保存されていることが明らかになった。
日本学術振興会, 基盤研究(B), 北海道大学, 20H03286

Elucidation of molecular mechanisms of phase transition of axillary meristems
Grants-in-Aid for Scientific Research
30 Oct. 2020 - 31 Mar. 2022
楢本 悟史
被子植物は頂端分裂組織の側方で葉を分化させるとともに、そのつけ根に腋芽を分化させる。発生の初期に形成される腋芽は無限成長性を示し、「枝メリステム」として、枝を伸張させながら葉と腋芽のペアを繰り返し形成する。この周期的発生は発生の進行にともない変調し、最終的には「花メリステム」とよばれる有限成長性を示す腋芽が形成され、花器官を分化させ、発生を停止する。本プロセスは、植物のシュート構造の多様性や穀物の結実数に直結することことから、重要な生命現象であるが、その分子メカニズムは未だ十分には明らかにされていない。これまでに我々は、イネの枝別れに関与する転写因子TAW1を見出している。そこで、TAW1の機能に注目することで、枝分かれサイクルの維持機構、ならびに、花メリステム形成に変調する機構を明らかにすることを目指し、研究を行った。
これまでの研究から、TAW1は、AP2型転写因子であるESR1やFZPの発現制御を介して、花メリステム形成への変調を抑制する作用を持つとの仮説を得ている。そこで本年度は、この仮説の検証を目標とした実験を行った。これまでに、ChIP解析により、TAW1がESR1の発現領域に直接結合することを見出した。また、esr1変異体では、茎頂分裂組織の活性が維持できなくなることを見出した。一方、ESR1は、オーキシンの合成やシグナル伝達を介して、葉の葉身の分化を制御することを見出した。加えて、発生・成長過程を通じて、ESR1は分裂組織では遺伝子発現せず、葉原基に特異的に発現することを明らかにした。以上のことから、ESR1は、細胞非自律的に茎頂分裂組織の活性の維持を制御することが示唆された。この仕組みはシロイヌナズナESR1や、トマトのオルソログでは見出されていないことから、イネが進化の過程で独自に獲得したメカニズムと考えられる。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Hokkaido University, 20H05403

新しいオーキシン極性輸送機構の解明：分子・細胞・個体から進化まで
科学研究費助成事業
2020 - 2020
楢本 悟史
日本学術振興会, 国際共同研究加速基金(国際共同研究強化(A)), 北海道大学, 20KK0337

Molecular mechanisms of axis formation in plants mediated by the regulation of lateral diffusion of plasma membrane proteins
Grants-in-Aid for Scientific Research
01 Apr. 2017 - 31 Mar. 2019
Naramoto Satoshi
My previous research identified the formation of dot-like clusters of PIN proteins at PMs. It also identified that PIN clusters are established or maintained by the cell wall components and the unknown function protein MAB4. Here I analyzed the role of MAB4 and cell wall in PIN cluster formation. I also performed forward genetic and chemical genetic screening to further elucidate the mechanism of PIN cluster formation.
I identified that MAB4 and PIN proteins both formed cluster-like structures at PMs. I also identified that they are largely colocalized. These findings suggested that MAB4 directly regulate PIN cluster formation. In contrast, I could not get clear evidence that demonstrate the direct involvement of cell wall in PIN cluster formation. Regarding the screening, I identified that phospholipids composition as well as the ATP biosynthesis play important roles for PIN cluster formation. I also succeeded to isolate one mutant that display defects in PIN cluster formation.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Tohoku University, 17K17595

Studies on molecular mechanism underlying photoperiodic compensation
Grants-in-Aid for Scientific Research
01 Apr. 2013 - 31 Mar. 2017
MIZOGUCHI Tsuyoshi
Circadian clock ensures consistency in antenna size under different photoperiod conditions. We named this novel clock function “photoperiodic compensation.” Double loss of function of LHY and CCA1 (lhy;cca1) resulted in changes in the total chlorophyll (Chl) level and Chl a/b ratio depending on the photoperiod. Under continuous light conditions (LL), the total Chl level increased significantly and the Chl a/b ratio decreased in lhy;cca1 compared with those in the wild type (WT). Increased stacked grana thylakoid membranes in chloroplasts were also observed in lhy;cca1 under LL. LIGHT HARVESTING COMPLEX II, which is an antenna protein involved in photosynthesis, accumulated to high levels in lhy;cca1 under LL compared with the WT.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), International Christian University, 25440141

Comprehensive omics analysis of plant cell wall formation
Grants-in-Aid for Scientific Research
28 Jun. 2012 - 31 Mar. 2017
DEMURA Taku; YONEDA Arata; YAMAGUCHI Masatoshi; NARAMOTO Satoshi
We performed a series of omics analyses on plant cell wall formation. Transcriptome/metabolome analysis revealed that the primary metabolism is strongly activated during secondary cell wall formation. Also, proteome analysis showed that the amount of several enzymes related to lignin biosynthesis and pectin degradation is drastically changed during secondary cell wall formation. On the analysis of primary cell wall formation, we established a system in which primary cell wall regeneration is induced from protoplasts of Arabidopsis cultured cells and mesophyll cells, using which we showed that KORRIGAN2 protein is closely associated with primary cell wall regeneration through transcriptome analysis and mutant analysis. Furthermore, we succeeded in indicating that in a moss plant, Physcomitrella patens, differentiation of water conducting cells (hydrioids) and supporting cells (stereids) is positively controlled by VNS genes, P. patens homologues of VND7.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), Nara Institute of Science and Technology, 24114002

Multiple approach on Study on the mechanism of polar localization of auxin efflux carrier PIN protein
Grants-in-Aid for Scientific Research
01 Apr. 2013 - 31 Mar. 2016
NARAMOTO SATOSHI
To elucidate the molecular mechanism of cell polarity establishment in plants, I analyzed the detailed distribution of PIN proteins at plasma membranes (PM)s. I identified that PIN proteins formed dot-like clusters at PMs and they are stable cellular compartments that do not diffuse across PMs. I also identified that PIN clusters are established or maintained by the cell wall components as well as the unknown function protein MAB4. Furthermore, I analyzed the van2, van5 and van6 mutants that are defective in vein development，related to polar auxin transport. Based on whole genome sequence analysis, I identified several candidates of responsible genes for van2, van5 and van6 mutants. Within them, I identified that responsible gene of van5 mutants is involved in sterol biosynthesis, which further confirms that appropriate lipid compositions of membranes are critical to establish cell polarity.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), 25840101

Study of plant cell polarity establishment by using advanced imaging and sequencing technology
Grants-in-Aid for Scientific Research
2011 - 2012
NARAMOTO Satoshi
To elucidate the mechanism of plant cell polarity establishment, subcellular localization analysis of GNOM and VAN3, vesicle transport regulators, and genetic analysis using arabidopsis mutants were performed. Whereas GNOM and VAN3 colocalized at PMs, they showed differential localization intracellularly. Interestingly, GNOM localization at non-endosomal compartments was also observed. Additionally, some mutation that supposed to be involved in cell polarity establishment was identified by next generation sequencing.
Japan Society for the Promotion of Science, Grant-in-Aid for Research Activity Start-up, 23870039

シロイヌナズナvan突然変異体を用いた維管束の連続性を制御する小胞輸送機構の研究
科学研究費助成事業
2005 - 2006
楢本 悟史
これまでにオーキシンの細胞間における極性輸送により,維管束パターンが制御されることが示されてきたもののその過程で機能する分子機構は不明な点が多かった.そこで私は維管束パターン,特に連続1生構築機構の解明を目的に,葉脈の不連続性を示すシロイヌナズナvan3の解析を行った.VAN3は小胞輸送の制御因子であるARF-GAPをコードすることから,細胞生物学的解析を行った.
VAN3は一部のTGNに局在するという,特殊な細胞内局在を示すにも関わらず,その局在化機構は不明であったことから,VAN3の各ドメインにGFPを融合させたタンパク質の細胞内局在を解析した.その結果,BAR, PHの両方のドメインを含む領域がVAN3の細胞内局在に重要な役割を担うことが明らかとなった.
また,VAN3が有するPHドメインはPI4-Pと顕著に相互作用することが明らかとなっており,VAN3のPHドメインは,TGN膜中のPI4-Pに富んだ領域にVAN3を局在化させる機能を有する可能性が考えられた.そこでこのことを明らかにするために,PI4-Pの生産に関与する5-PaseであるCVP2,とVAN3との関係を解析した.まず,cvp2においてVAN3の細胞内局在を調べた所,顕著な異常は観察されずCol下と同様にTGNに局在していた.一方,van3-2変異を有する変異型VAN3は,Col下においては顕著な局在の異常は観察されないにも関わらず,興味深いことにcvp2ではTGN膜へ局在不能となり,細胞質に局在が観察された.私は遺伝学的解析からも,cvp2変異がvan3-2の表現型を亢進する実験結果を得ており,以上の結果からCVP2が生産するPI4-PがVAN3の有するPHドメインに作用し,VAN3のTGN膜への局在を規定する分子機構が存在することを明らかにした.
日本学術振興会, 特別研究員奨励費, 東京大学, 05J11468

Apr. 2017 - Apr. 2017
木質バイオマス有効利用へ向けた植物細胞壁改変技術の開発
木質バイオマス有効利用へ向けた，植物細胞壁改変技術を開発する．

Apr. 2016 - Apr. 2016
オーキシン排出担体PINの極性局在を制御する細胞膜ドメイン形成機構の研究
オーキシン排出担体PINの極性局在を制御する細胞膜ドメイン形成機構に関して，分子細胞生物学的に研究を行う．

Jan. 2016 - Jan. 2016
植物の極性構築メカニズムの分子細胞生物学的研究
植物の極性構築メカニズムに関して分子細胞生物学的に解析する．

Nov. 2014 - Nov. 2014
植物細胞璧と細胞極性の関わり〜細胞内小胞輸送を中心として
植物細胞璧を基盤とした植物細胞の極性形成の分子機構を明らかにする．