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Ito Hidetaka

Faculty of Science　Biological Sciences　Cell Structure and FunctionAssociate Professor
Institute for Academic InnovationAssociate Professor

Researcher basic information

■ Degree
  • Doctor of Agriculture, Kyoto University, Mar. 2004
■ URL
researchmap URLホームページURL■ Various IDs
J-Global ID■ Research Keywords and Fields
Research Keyword
  • VOC
  • Genome evolution
  • Epigenetics
  • Environmental stress
  • Transposon
  • evolution
  • epigenetics
  • stress
  • transposon
Research Field
  • Life Science, Plant molecular biology and physiology
  • Life Science, Genetics
■ Educational Organization

Career

■ Career
Career
  • Apr. 2024 - Present
    Hokkaido University, Nitobe College, Educational Support Manager (concurrent)
  • Oct. 2019 - Present
    Hokkaido University, Graduate School of Science, Associate Professor
  • Apr. 2018 - Mar. 2024
    Hokkaido University, Nitobe College, Organization for the Advancement of Higher Education, Associate Professor (concurrent)
  • Apr. 2010 - Sep. 2019
    Hokkaido University, Graduate School of Science, Assistant Professor
  • Oct. 2011 - Mar. 2015
    Japan Science and Technology Agency, Research Fellow (part-time)
  • 2007 - 2010
    Researcher, University of Geneva, Switzerland
  • 2004 - 2007
    National Institute of Genetics, Researcher, National Institute of Genetics
Educational Background
  • Apr. 2001 - Mar. 2004, Kyoto University, Graduate School of Agriculture, Department of Applied Life Sciences, Japan
  • Apr. 1999 - Mar. 2001, Kyoto University, Faculty of Agriculture, Division of Applied Biosciences, Japan
  • Apr. 1995 - Mar. 1999, Kyoto University, Faculty of Agriculture, 生物生産学科, Japan
Committee Memberships
  • Apr. 2023 - Mar. 2025
    日本遺伝学会, 評議委員, Society
  • Apr. 2017 - Mar. 2021
    The Genetics Society of Japan, Trustees, Society

Research activity information

■ Awards
  • Feb. 2024, 北海道大学, 令和5年度 教育研究総長表彰
    伊藤 秀臣
  • Sep. 2022, 日本遺伝学会, 日本遺伝学会第94回大会Best Paper賞
    DDR複合体による熱活性型レトロトランスポゾンの制御機構
    牛小蛍;陳露;加藤敦之;伊藤秀臣, Japan society, Japan
  • Sep. 2021, The genetics society of Japan, GGS PRIZE 2021
    ONSEN shows different transposition activities in RdDM pathway mutants
    Yui Hayashi;Kanako Takehira;Kosuke Nozawa;Takamasa Suzuki;Yukari;Masuta;Atsushi Kato;Hidetaka Ito, Official journal, Japan
  • Aug. 2021, 日本遺伝学会, The Genetics Society of Japan Incentive Award for 2021
    Studies on environmental stress-activated transposons
    Hidetaka Ito, Japan society, Japan
  • Sep. 2010, The genetics society of Japan, Best Paper Award at the 82nd Annual Meeting of the Genetics Society of Japan
    siRNA pathway control transgenerational transposition of heat activated retrotransposon in Arabidopsis
    Hidetaka Ito, Japan society, Japan
■ Papers
  • Heat-responsive ONSEN long terminal repeats integrate heat shock factor motifs, DNA methylation and natural sequence variation in Arabidopsis
    Baibhav R. Barbaruah; Rahmadani P. Airlangga; Hidetaka Ito
    Biology Open, 21 Aug. 2026, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    Scientific journal
  • RHE1 is associated with short-day-induced hypocotyl elongation, peroxisomal ROS homeostasis, and m 6A-dependent RNA stability in Arabidopsis
    Yibin Lin; Yijun Meng; Hidetaka Ito
    Plant and Cell Physiology, Oxford University Press (OUP), 28 Jul. 2026, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    English, Scientific journal, Abstract

    Plants dynamically adjust growth in response to short-day (SD) conditions while maintaining reactive oxygen species (ROS) homeostasis across cellular compartments. However, how light signaling is coupled to organelle-specific redox regulation remains poorly understood. Here, we identify the peroxisomal Mpv17/PMP22 family protein REGULATOR OF ROS-MEDIATED HYPOCOTYL ELONGATION 1 (RHE1) as a regulator of short-day-induced hypocotyl elongation and ROS homeostasis in Arabidopsis thaliana. RHE1 expression is associated with a light-responsive G-box element bound by HY5 and shows a strong positive relationship with N6-methyladenosine (m6A) enrichment. Environmental stresses that elevate inferred m6A levels increase RHE1 transcript abundance and stability, whereas short-day conditions reduce m6A enrichment and decrease RHE1 expression. Loss of RHE1 results in enhanced hypocotyl cell elongation and elevated ROS accumulation specifically under short-day conditions, indicating that RHE1 constrains growth through peroxisomal redox regulation. Furthermore, RHE1 transcript levels correlate with the m6A reader ECT2, and published binding datasets support direct association of ECT2 with RHE1 mRNA. Together, these findings reveal a multilayered regulatory mechanism in which HY5-associated transcription and m6A-dependent RNA stabilization converge on RHE1 to coordinate SD-dependent growth with peroxisomal ROS homeostasis. This study uncovers a previously unrecognized connection between SD signaling, epitranscriptomic regulation, and organelle-specific redox control.
  • Gene-body methylation conservation is associated with volatile-induced transcriptional responsiveness in Arabidopsis thaliana
    Baibhav R. Barbaruah; Yuga Harada; Hidetaka Ito
    Plant Gene, 47, 100602, 100602, Elsevier BV, 15 Jul. 2026, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    English, Scientific journal, 50683450
  • Ecotype-specific sensitivity to DNA methylation inhibitors in Arabidopsis cmt3 mutants
    Xin Sun; Guo Du; Atsushi Kato; Hidetaka Ito
    Molecular Genetics and Genomics, 20 May 2026, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    Scientific journal
  • A short trans-2-hexenal pulse primes freezing tolerance and a cold-response transcriptome in Arabidopsis thaliana
    Baibhav R. Barbaruah; Hidetaka Ito
    Gene, 987, 150032, 150032, Elsevier BV, 15 Apr. 2026, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    English, Scientific journal, 50683450
  • Natural variation uncouples HSFA2 induction from volatile-induced thermotolerance in Arabidopsis thaliana
    B. R. Barbaruah; S. Feng; S. Chen; H. Ito
    Plant Molecular Biology, 26 Mar. 2026, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    English, Scientific journal, 50683450
  • Volatile Organic Compounds Prime Heat Tolerance Through Chromatin-Dependent Heat Shock Pathways in Arabidopsis
    Baibhav Raj Barbaruah; S Feng; H Ito
    Plant and Cell Physiology, 67, 6, 1037, 1051, Oxford University Press (OUP), 28 Feb. 2026, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    English, Scientific journal, Abstract

    Volatile organic compounds (VOCs) can prime plants for improved survival under heat stress, yet how chromatin regulation shapes VOC-triggered heat-shock responses remains unclear. Here, we analyzed heat and VOC responses in Arabidopsis thaliana Col-0 and the epigenetic mutants ddm1, nrpd1, suvh456 and hda6. In a seven-VOC screen, heat and multiple VOCs induced HSFA2, with trans-2-hexenal (T2H) among the most consistent inducers. Although ddm1 and hda6 showed reduced HSFA2 induction under heat, VOC induction remained strong, indicating that volatile signaling can partially re-balance heat-dependent regulation. Priming with the VOC panel increased survival after lethal heat across genotypes, and phenotype scoring (Healthy/Affected/Dead) revealed genotype-dependent outcome shifts, including a stronger T2H-driven enrichment of Healthy seedlings in hda6. Expression profiling of HSFA1a-e regulators and the downstream chaperone HSP101 indicated intact pathway competence, while revealing quantitative rewiring in hda6. RNA-seq showed that T2H activates a programme that is distinct from, but partially overlaps with, the heat response, combining a shared stress core with VOC-biased signaling and reactive oxygen species (ROS)-related modules that are amplified in hda6. Finally, analysis of public ChIP-seq datasets linked altered promoter enrichment of active marks (H3Ac and H3K4me2), particularly at HDA6-bound promoters, to basal transcript output and uneven tuning of VOC- and heat/ROS-responsive loci. Together, these results support a model in which chromatin regulation tunes both the basal state and inducibility of VOC-responsive stress networks, thereby shaping VOC-primed thermotolerance., 50683450
  • The transposition of a heat-activated retrotransposon ONSEN resulted in changes in the hypocotyl elongation.
    Ryu Hasegawa; Hidetaka Ito
    Genes & genetic systems, 25 Jan. 2025, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
    English, Scientific journal, In our study, we aimed to identify new mutants resulting from ONSEN transposition in Arabidopsis thaliana by subjecting nrpd1 mutants to heat stress. We isolated a mutant with a significantly elongated hypocotyl, named "Long hypocotyl in ONSEN inserted line 1" (HYO1). This phenotype was heritable, with progeny consistently displaying longer hypocotyls than the wild type. Genetic analysis revealed that this trait was due to a single recessive mutation. Further mapping and sequencing identified the insertion of ONSEN in the HY2 gene, a crucial regulator of hypocotyl elongation. The insertion disrupted HY2 transcription, as confirmed by quantitative PCR, leading to the observed phenotype. To assess the influence of the nrpd1 background, we generated lines backcrossed twice to wild-type Col-0, and the results were consistent with those observed in the original mutant lines. Furthermore, we examined the effect of HY2 and HYO1 mutations on flowering time by analyzing the expression levels of FT. The hyo1 mutant exhibited earlier flowering compared to both the wild type and nrpd1 mutants, with increased FT expression levels. This research underscores the significant impact of ONSEN transposition on gene function and phenotypic variation in Arabidopsis thaliana, providing new insights into the mutagenic potential of transposons and their role in shaping plant traits.
  • Centrophilic retrotransposon integration via CENH3 chromatin in Arabidopsis
    Sayuri Tsukahara; Alexandros Bousios; Estela Perez-Roman; Sota Yamaguchi; Basile Leduque; Aimi Nakano; Matthew Naish; Akihisa Osakabe; Atsushi Toyoda; Hidetaka Ito; Alejandro Edera; Sayaka Tominaga; Juliarni; Kae Kato; Shoko Oda; Soichi Inagaki; Zdravko Lorković; Kiyotaka Nagaki; Frédéric Berger; Akira Kawabe; Leandro Quadrana; Ian Henderson; Tetsuji Kakutani
    Nature, Springer Science and Business Media LLC, 01 Jan. 2025, [Peer-reviewed], [International Magazine]
    Scientific journal
  • Regulatory mechanism of heat-active retrotransposons by the SET domain protein SUVH2
    Xiaoying Niu; Zhiyu Ge; Hidetaka Ito
    Frontiers in Plant Science, 15, frontiers, 08 Feb. 2024, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    English, Scientific journal
  • Regulatory mechanism of a heat-activated retrotransposon by DDR complex in Arabidopsis thaliana
    Xiaoying Niu; Lu Chen; Atsushi Kato; Hidetaka Ito
    Frontiers in Plant Science, 13, Frontiers Media SA, 21 Dec. 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    English, Scientific journal, The RNA-directed DNA methylation (RdDM) pathway plays an essential role in the transposon silencing mechanism; the DDR complex, consisting of DRD1, DMS3, and RDM1, is an essential component of the RdDM pathway. ONSEN, identified in Arabidopsis, is a retrotransposon activated by heat stress at 37°C; however, studies on the regulation of ONSEN are limited. In this study, we analyzed the regulation of ONSEN activity by the DDR complex in Arabidopsis. We elucidated that loss of any component of the DDR complex increased ONSEN transcript levels. Transgenerational transposition of ONSEN was observed in the DDR-complex mutants treated with heat stress for 48 h. Furthermore, the DDR complex components DRD1, DMS3, and RDM1 played independent roles in suppressing ONSEN transcription and transposition. Moreover, we found that the duration of heat stress affects ONSEN activity. Therefore, the results of this study provide new insights into the retrotransposon regulatory mechanisms of the DDR complex in the RdDM pathway.
  • Environmental stress and transposons in plants
    Hidetaka Ito
    Genes & genetic systems, 97, 4, 169, 175, Genetics Society of Japan, 04 Aug. 2022, [Peer-reviewed], [Invited], [Lead author, Last author, Corresponding author], [International Magazine]
    Scientific journal
  • Diversity and strategies of environmental stress-responsive transposons in plants.
    Hidetaka Ito
    Genes & genetic systems, 97, 4, 167, 167, Aug. 2022, [Lead author, Last author, Corresponding author], [International Magazine]
    English, Scientific journal
  • Epigenetic regulation of ecotype-specific expression of the heat-activated transposon ONSEN
    Kosuke Nozawa; Seiji Masuda; Hidetoshi Saze; Yoko Ikeda; Takamasa Suzuki; Hiroki Takagi; Keisuke Tanaka; Naohiko Ohama; Xiaoying Niu; Atsushi Kato; Hidetaka Ito
    Frontiers in Plant Science, 13, Frontiers Media SA, 18 Jul. 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    English, Scientific journal, Transposable elements are present in a wide variety of organisms; however, our understanding of the diversity of mechanisms involved in their activation is incomplete. In this study, we analyzed the transcriptional activation of the ONSEN retrotransposon, which is activated by high-temperature stress in Arabidopsis thaliana. We found that its transcription is significantly higher in the Japanese ecotype Kyoto. Considering that transposons are epigenetically regulated, DNA methylation levels were analyzed, revealing that CHH methylation was reduced in Kyoto compared to the standard ecotype, Col-0. A mutation was also detected in the Kyoto CMT2 gene, encoding a CHH methyltransferase, suggesting that it may be responsible for increased expression of ONSEN. CHH methylation is controlled by histone modifications through a self-reinforcing loop between DNA methyltransferase and histone methyltransferase. Analysis of these modifications revealed that the level of H3K9me2, a repressive histone marker for gene expression, was lower in Kyoto than in Col-0. The level of another repressive histone marker, H3K27me1, was decreased in Kyoto; however, it was not impacted in a Col-0 cmt2 mutant. Therefore, in addition to the CMT2 mutation, other factors may reduce repressive histone modifications in Kyoto.
  • The novel activity of Argonautes in intron splicing: A transcriptome-wide survey in plants
    Yijun Meng; Xiaoxia Ma; Jie Li; Hidetaka Ito; Krystyna Oracz; Jiahui Cai; Chaogang Shao
    Journal of Plant Physiology, 270, 153632, 153632, Elsevier BV, 31 Jan. 2022, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
    English, Scientific journal, The importance of the evolutionarily conserved Argonaute (AGO) proteins has been well recognized for their involvement in the RNA interference pathways. Recent discoveries in animals demonstrated that AGOs also participate in alternative splicing (AS). Motivated by the question whether the AGO proteins are also functional in RNA splicing in plants, we searched for the introns excised through an AGO-dependent manner in Arabidopsis (Arabidopsis thaliana). RNA sequencing (RNA-seq) data analysis uncovered hundreds of the introns up- or down-regulated in the ago1 and ago4 mutants, respectively. For different genes, AGOs might play either a positive or a negative role in intron excision, which was further validated by reverse transcription-polymerase chain reaction (RT-PCR). Some introns were specifically regulated by one of the AGO proteins, while some were regulated by both AGOs. Besides, a large portion of the AGO-dependent introns were organ-specifically regulated. RNA immunoprecipitation combined with high-throughput sequencing (RIP-seq) revealed that both AGOs preferentially bound to the intronic regions, supporting their high intron binding affinities. Immunoprecipitation followed by mass spectrometry (IP-MS) was performed to identify the proteins potentially interacting with the two AGOs. Six novel interactors (two interacting with AGO1 and four with both AGOs) involved in mRNA binding were uncovered, which might facilitate AGO–intron recognition. Analysis of the RNA-seq data from the rice (Oryza sativa) ago18 mutants revealed that hundreds of the introns were expressed in an AGO18-dependent manner. In summary, our results point to the novel role of the plant AGOs in intron splicing, paving a way for further studies on the mechanisms underlying AGO-mediated RNA splicing.
  • DNA methyltransferase CHROMOMETHYLASE3 prevents ONSEN transposon silencing under heat stress
    Kosuke Nozawa; Jiani Chen; Jianjun Jiang; Sarah M. Leichter; Masataka Yamada; Takamasa Suzuki; Fengquan Liu; Hidetaka Ito; Xuehua Zhong
    PLOS Genetics, 17, 8, e1009710, e1009710, Public Library of Science (PLoS), 19 Aug. 2021, [Peer-reviewed], [Corresponding author], ［Internationally co-authored］, [International Magazine]
    English, Scientific journal, DNA methylation plays crucial roles in transposon silencing and genome integrity. CHROMOMETHYLASE3 (CMT3) is a plant-specific DNA methyltransferase responsible for catalyzing DNA methylation at the CHG (H = A, T, C) context. Here, we identified a positive role of CMT3 in heat-induced activation of retrotransposon ONSEN. We found that the full transcription of ONSEN under heat stress requires CMT3. Interestingly, loss-of-function CMT3 mutation led to increased CHH methylation at ONSEN. The CHH methylation is mediated by CMT2, as evidenced by greatly reduced CHH methylation in cmt2 and cmt2 cmt3 mutants coupled with increased ONSEN transcription. Furthermore, we found more CMT2 binding at ONSEN chromatin in cmt3 compared to wild-type accompanied with an ectopic accumulation of H3K9me2 under heat stress, suggesting a collaborative role of H3K9me2 and CHH methylation in preventing heat-induced ONSEN activation. In summary, this study identifies a non-canonical role of CMT3 in preventing transposon silencing and provides new insights into how DNA methyltransferases regulate transcription under stress conditions.
  • DRD1, a SWI/SNF-like chromatin remodeling protein, regulates a heat-activated transposon in Arabidopsis thaliana
    Kanako Takehira; Yui Hayashi; Kosuke Nozawa; Lu Chen; Takamasa Suzuki; Yukari Masuta; Atsushi Kato; Hidetaka Ito
    Genes & genetic systems, 96, 3, 151, 158, Genetics Society of Japan, 08 Aug. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    English, Scientific journal
  • How to Activate Heat-Responsible Retrotransposon ONSEN in Brassicaceae Species.
    Hidetaka Ito
    Methods in molecular biology (Clifton, N.J.), 2250, 189, 194, 27 Apr. 2021, [Peer-reviewed], [Invited], [Lead author, Last author, Corresponding author], [International Magazine]
    English, Scientific journal, A number of transposable elements are activated by environmental stress. A Ty1/copia-type retrotransposon named ONSEN is activated by heat stress in Brassicaceae species. A synthetic activation of the transposon is effective for the molecular breeding without genetic modification. Here, we described the detail procedure of heat treatment to activate ONSEN in Brassicaceae species.
  • ONSEN shows different transposition activities in RdDM pathway mutants.
    Yui Hayashi; Kanako Takehira; Kosuke Nozawa; Takamasa Suzuki; Yukari Masuta; Atsushi Kato; Hidetaka Ito
    Genes & genetic systems, 95, 4, 183, 190, 05 Sep. 2020, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
    English, Scientific journal, Most transposable elements (TEs) are tightly regulated by epigenetic mechanisms such as DNA methylation. RNA-directed DNA methylation (RdDM) is a major control mechanism of TE silencing in plants. We analyzed the transposition activity of a heat-responsive retrotransposon, ONSEN, in Arabidopsis thaliana. Transgenerational transposition was observed in RdDM pathway-deficient mutants upon heat stress. The transposition frequency was higher in the mutants of the upstream processes, but lower in the mutants of the downstream steps, of RdDM. The transposition frequency was not associated with the number of extrachromosomal ONSEN copies. Constitutive heterochromatin of interphase nuclei was dispersed upon heat stress. The degree of decondensation was higher in the RdDM mutants than in wild-type plants subjected to heat stress. We discuss the possible role of RdDM in the regulation of ONSEN transposition upon heat stress., 11473815
  • The RNA degradome: a precious resource for deciphering RNA processing and regulation codes in plants.
    Xiaoxia Ma; Xiaopu Yin; Zhonghai Tang; Hidetaka Ito; Chaogang Shao; Yijun Meng; Tian Xie
    RNA biology, 17, 9, 1223, 1227, Sep. 2020, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
    English, Scientific journal, The plant RNA degradome was defined as an aggregate of the RNA fragments degraded from various biochemical pathways, such as RNA turnover, maturation and quality surveillance. In recent years, the degradome sequencing (degradome-seq) libraries became a rich storehouse for researchers to study on RNA processing and regulation. Here, we provided a brief overview of the uses of degradome-seq data in plant RNA biology, especially on non-coding RNA processing and small RNA-guided target cleavages. Some novel applications in RNA research area, such as in vivo mapping of the endoribonucleolytic cleavage sites, identification of conserved motifs at the 5' ends of the uncapped RNA fragments, and searching for the protein-binding regions on the transcripts, were also mentioned. More importantly, we proposed a model for the biologists to deduce the contributions of transcriptional and/or post-transcriptional regulation to gene differential expression based on degradome-seq data. Finally, we hope that the degradome-based analytical methods could be widely applied for the studies on RNA biology in eukaryotes.
  • The effect of zebularine on the heat-activated retrotransposon ONSEN in Arabidopsis thaliana and Vigna angularis.
    Patwira Boonjing; Yukari Masuta; Kosuke Nozawa; Atsushi Kato; Hidetaka Ito
    Genes & genetic systems, 95, 4, 165, 172, 03 Aug. 2020, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
    English, Scientific journal, The Ty1/copia-like retrotransposon ONSEN is conserved among Brassica species, as well as in beans, including adzuki bean (Vigna angularis (Willd.) Ohwi & Ohashi), which is one of the economically important crops in Japan. ONSEN has acquired a heat-responsive element that is recognized by plant heat stress defense factors, resulting in its transcription and the production of full-length extrachromosomal DNA under conditions with elevated temperatures. DNA methylation plays an important role in regulating the activation of this transposon in plants. Therefore, chemical inhibition of DNA methyltransferases has been utilized to study the effect of DNA methylation on transposon activation. To understand the effect of DNA methylation on ONSEN activation, Arabidopsis thaliana and adzuki bean seedlings were treated with zebularine, which is known to be an effective chemical demethylation agent. The results showed that ONSEN transcription levels were upregulated in zebularine-treated plants. Extrachromosomal DNA of ONSEN also accumulated in the treated plants., 11473817
  • PmiRDiscVali: an integrated pipeline for plant microRNA discovery and validation.
    Dongliang Yu; Ying Wan; Hidetaka Ito; Xiaoxia Ma; Tian Xie; Tingzhang Wang; Chaogang Shao; Yijun Meng
    BMC genomics, 20, 1, 133, 133, 13 Feb. 2019, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
    English, Scientific journal, BACKGROUND: MicroRNAs (miRNAs) constitute a well-known small RNA (sRNA) species with important regulatory roles. To date, several bioinformatics tools have been developed for large-scale prediction of miRNAs based on high-throughput sequencing data. However, some of these tools become invalid without reference genomes, while some tools cannot supply user-friendly outputs. Besides, most of the current tools focus on the importance of secondary structures and sRNA expression patterns for miRNA prediction, while they do not pay attention to miRNA processing for reliability check. RESULTS: Here, we reported a pipeline PmiRDiscVali for plant miRNA discovery and partial validation. This pipeline integrated the popular tool miRDeep-P for plant miRNA prediction, making PmiRDiscVali compatible for both reference-based and de novo predictions. To check the prediction reliability, we adopted the concept that the miRNA processing intermediates could be tracked by degradome sequencing (degradome-seq) during the development of PmiRDiscVali. A case study was performed by using the public sequencing data of Dendrobium officinale, in order to show the clear and concise presentation of the prediction results. CONCLUSION: Summarily, the integrated pipeline PmiRDiscVali, featured with degradome-seq data-based validation and vivid result presentation, should be useful for large-scale identification of plant miRNA candidates.
  • MepmiRDB: a medicinal plant microRNA database.
    Dongliang Yu; Jiangjie Lu; Weishan Shao; Xiaoxia Ma; Tian Xie; Hidetaka Ito; Tingzhang Wang; Min Xu; Huizhong Wang; Yijun Meng
    Database : the journal of biological databases and curation, 2019, 01 Jan. 2019, [Peer-reviewed], [International Magazine]
    English, Scientific journal, MicroRNAs (miRNAs) have been recognized as a key regulator in plant development and metabolism. Recent reports showed that the miRNAs of medicinal plants not only act as a critical modulator in secondary metabolism but also had a great potential of performing cross-kingdom gene regulation. Although several plant miRNA repositories have been publicly available, no miRNA database specific for medicinal plants has been reported to date. Here, we report the first version of MepmiRDB (medicinal plant microRNA database), which is freely accessible at http://mepmirdb.cn/mepmirdb/index.html. This database accommodates thousands of miRNA candidates belonging to 29 medicinal plant species. The miRNA information on sequences, expression patterns and regulatory networks has been included in the functional modules of the database. Specifically, the 'Sequence' module provides the sequences of the mature miRNAs and their precursors, and the structure information of the precursors. Moreover, the processing and small RNA accumulation signals on the miRNA precursors are also included in the 'Sequence' module. The organ/growth condition-specific expression information of the mature miRNAs has been stored in the 'Expression' module. The 'Interaction' module offers the information of the degradome-validated miRNA-target pairs of eight plant species. The 'Search' module enables users to search for the miRNAs by plant species and miRNA families, or by sequences. All data in this database are available for download. Taken together, the functional modules of MepmiRDB ensure its importance and timeliness for mechanistic and functional studies on the medicinal plant miRNAs.
  • Deciphering the non-coding RNA-level response to arsenic stress in rice (Oryza sativa).
    Zhonghai Tang; Min Xu; Hidetaka Ito; Jiahui Cai; Xiaoxia Ma; Jingping Qin; Dongliang Yu; Yijun Meng
    Plant signaling & behavior, 14, 9, 1629268, 1629268, 2019, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Arsenic (As) contamination in subsoil and groundwater is a big problem, especially in many South-East Asian countries. As a staple crop growing under flooded condition in these areas, rice (Oryza sativa L.) becomes a big threat to human health through the food chain since As is highly accumulated in grains. Thus, reducing As accumulation in rice through molecular breeding and identification of rice varieties with low As content are the pressing issues. However, the current understanding on the molecular mechanism of As stress response is still limited for rice. In this study, we performed a comprehensive search for the As-responsive small RNAs (sRNAs) of rice. Briefly, 4,762 and 18,152 sRNAs were identified to be highly activated under As stress in roots and shoots respectively, while 14,603 and 8,308 sRNAs were intensively repressed by As treatment in roots and shoots, respectively. A number of the As-responsive sRNAs found their loci on tRNAs, rRNAs or long non-coding RNAs (lncRNAs). Interestingly, these loci preferentially distributed on the 5' halves of the tRNA, rRNA or lncRNA precursors. Among the above-identified As-responsive sRNAs, 252 Argonaute 1 (AGO1)-enriched sRNAs were extracted for target identification, resulting in 200 pairs of sRNA-protein-coding target interactions. Many targets are functionally involved in the development, stress response, reproduction, or lipid metabolism. Additionally, 56 lncRNAs were discovered to be targeted by nine AGO1-enriched sRNAs, indicating the potential involvement of these lncRNAs in As signaling. Taken together, our results could expand the understanding on the non-coding RNA-mediated As stress response in rice.
  • Characterization of a heat-activated retrotransposon in Vigna angularis.
    Yukari Masuta; Akira Kawabe; Kosuke Nozawa; Ken Naito; Atsushi Kato; Hidetaka Ito
    Breeding science, 68, 2, 168, 176, Japanese Society of Breeding, Mar. 2018, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
    English, Scientific journal
  • Tracking microRNA Processing Signals by Degradome Sequencing Data Analysis.
    Dongliang Yu; Min Xu; Hidetaka Ito; Weishan Shao; Xiaoxia Ma; Huizhong Wang; Yijun Meng
    Frontiers in genetics, 9, 546, 546, 2018, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Degradome sequencing (degradome-seq) was widely used for cleavage site mapping on the microRNA (miRNA) targets. Here, the application value of degradome-seq data in tracking the miRNA processing intermediates was reported. By adopting the parameter "signal/noise" ratio, prominent degradome signals on the miRNA precursors were extracted. For the 15 species analyzed, the processing of many miRNA precursors were supported by the degradome-seq data. We found that the supporting ratio of the "high-confidence" miRNAs annotated in miRBase was much higher than that of the "low-confidence." For a specific species, the percentage of the miRNAs with degradome-supported processing signals was elevated by the increment of degradome sampling diversity. More interestingly, the tissue- or cell line-specific processing patterns of the miRNA precursors partially contributed to the accumulation patterns of the mature miRNAs. In this study, we also provided examples to show the value of the degradome-seq data in miRNA annotation. Based on the distribution of the processing signals, a renewed model was proposed that the stems of the miRNA precursors were diced through a "single-stranded cropping" mode, and "loop-to-base" processing was much more prevalent than previously thought. Together, our results revealed the remarkable capacity of degradome-seq in tracking miRNA processing signals.
  • Epigenetic regulation of a heat-activated retrotransposon in Cruciferous Vegetables
    Nozawa Kosuke; Kawagishi Yuki; Kawabe Akira; Sato Mio; Masuta Yukari; Kato Atsushi; Ito Hidetaka
    Epigenomes, 1, 7, 7, 7, Jun. 2017, [Peer-reviewed], [Last author, Corresponding author]
    English, Scientific journal, Transposable elements (TEs) are highly abundant in plant genomes. Environmental stress is one of the critical stimuli that activate TEs. We analyzed a heat-activated retrotransposon, named ONSEN, in cruciferous vegetables. Multiple copies of ONSEN-like elements (OLEs) were found in all of the cruciferous vegetables that were analyzed. The copy number of OLE was high in Brassica oleracea, which includes cabbage, cauliflower, broccoli, Brussels sprout, and kale. Phylogenic analysis demonstrated that some OLEs transposed after the allopolyploidization of parental Brassica species. Furthermore, we found that the high copy number of OLEs in B. oleracea appeared to induce transpositional silencing through epigenetic regulation, including DNA methylation. The results of this study would be relevant to the understanding of evolutionary adaptations to thermal environmental stress in different species.
  • Characterization of a heat-activated retrotransposon in natural accessions of Arabidopsis thaliana.
    Seiji Masuda; Kosuke Nozawa; Wataru Matsunaga; Yukari Masuta; Akira Kawabe; Atsushi Kato; Hidetaka Ito
    Genes & genetic systems, 91, 6, 293, 299, 13 May 2017, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
    English, Scientific journal
  • Inducible Transposition of a Heat-Activated Retrotransposon in Tissue Culture.
    Yukari Masuta; Kosuke Nozawa; Hiroki Takagi; Hiroki Yaegashi; Keisuke Tanaka; Tasuku Ito; Hideyuki Saito; Hisato Kobayashi; Wataru Matsunaga; Seiji Masuda; Atsushi Kato; Hidetaka Ito
    Plant & cell physiology, 58, 2, 375, 384, 01 Feb. 2017, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
    English, Scientific journal, A transposition of a heat-activated retrotransposon named ONSEN required compromise of a small RNA-mediated epigenetic regulation that includes RNA-directed DNA methylation (RdDM) machinery after heat treatment. In the current study, we analyzed the transcriptional and transpositional activation of ONSEN to better understand the underlying molecular mechanism involved in the maintenance and/or induction of transposon activation in plant tissue culture. We found the transposition of heat-primed ONSEN during tissue culture independently of RdDM mutation. The heat activation of ONSEN transcripts was not significantly up-regulated in tissue culture compared with that in heat-stressed seedlings, indicating that the transposition of ONSEN was regulated independently of the transcript level. RdDM-related genes were up-regulated by heat stress in both tissue culture and seedlings. The level of DNA methylation of ONSEN did not show any change in tissue culture, and the amount of ONSEN-derived small RNAs was not affected by heat stress. The results indicated that the transposition of ONSEN was regulated by an alternative mechanism in addition to the RdDM-mediated epigenetic regulation in tissue culture. We applied the tissue culture-induced transposition of ONSEN to Japanese radish, an important breeding species of the family Brassicaceae. Several new insertions were detected in a regenerated plant derived from heat-stressed tissues and its self-fertilized progeny, revealing the possibility of molecular breeding without genetic modification.
  • A Stress-Activated Transposon in Arabidopsis Induces Transgenerational Abscisic Acid Insensitivity.
    Hidetaka Ito; Jong-Myong Kim; Wataru Matsunaga; Hidetoshi Saze; Akihiro Matsui; Takaho A Endo; Yoshiko Harukawa; Hiroki Takagi; Hiroki Yaegashi; Yukari Masuta; Seiji Masuda; Junko Ishida; Maho Tanaka; Satoshi Takahashi; Taeko Morosawa; Tetsuro Toyoda; Tetsuji Kakutani; Atsushi Kato; Motoaki Seki
    Scientific reports, 6, 23181, 23181, 15 Mar. 2016, [Peer-reviewed], [Lead author], [International Magazine]
    English, Scientific journal
  • AtRBP1, which encodes an RNA-binding protein containing RNA-recognition motifs, regulates root growth in Arabidopsis thaliana.
    Takuhiro Shida; Ai Fukuda; Tamao Saito; Hidetaka Ito; Atsushi Kato
    Plant physiology and biochemistry : PPB, 92, 62, 70, Jul. 2015, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • Role of the ACL2 locus in flower stalk elongation in Arabidopsis thaliana.
    Hiroaki Kato; Yoshibumi Komeda; Tamao Saito; Hidetaka Ito; Atsushi Kato
    Genes & genetic systems, 90, 3, 163, 74, 2015, [Peer-reviewed], [Domestic magazines]
    English, Scientific journal
  • A small RNA mediated regulation of a stress-activated retrotransposon and the tissue specific transposition during the reproductive period in Arabidopsis.
    Wataru Matsunaga; Naohiko Ohama; Noriaki Tanabe; Yukari Masuta; Seiji Masuda; Namiki Mitani; Kazuko Yamaguchi-Shinozaki; Jian F Ma; Atsushi Kato; Hidetaka Ito
    Frontiers in plant science, 6, 48, 48, 2015, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
    English, Scientific journal
  • Genomic localization of AtRE1 and AtRE2, copia-type retrotransposons, in natural variants of Arabidopsis thaliana.
    Mari Yamada; Yumi Yamagishi; Masashi Akaoka; Hidetaka Ito; Atsushi Kato
    Molecular genetics and genomics : MGG, 289, 5, 821, 35, Oct. 2014, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • Control of transposable elements in Arabidopsis thaliana.
    Hidetaka Ito; Tetsuji Kakutani
    Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 22, 2, 217, 23, Kluwer Academic Publishers, Jun. 2014, [Peer-reviewed], [Lead author], [International Magazine]
    English, Scientific journal
  • Overexpression of the TIR-X gene results in a dwarf phenotype and activation of defense-related gene expression in Arabidopsis thaliana.
    Hiroaki Kato; Tamao Saito; Hidetaka Ito; Yoshibumi Komeda; Atsushi Kato
    Journal of plant physiology, 171, 6, 382, 8, 15 Mar. 2014, [Peer-reviewed], [International Magazine]
    English, Scientific journal
  • Plant Models of Transgenerational Epigenetic Inheritance
    Hidetaka Ito
    Transgenerational Epigenetics, 147, 161, Elsevier Inc., 2014, [Peer-reviewed], [Lead author, Last author, Corresponding author]
    English, In book
  • Evolution of the ONSEN retrotransposon family activated upon heat stress in Brassicaceae.
    Hidetaka Ito; Takanori Yoshida; Sayuri Tsukahara; Akira Kawabe
    Gene, 518, 2, 256, 61, 15 Apr. 2013, [Peer-reviewed], [Lead author], [International Magazine]
    English, Scientific journal
  • Small RNAs and regulation of transposons in plants.
    Hidetaka Ito
    Genes & genetic systems, 88, 1, 3, 7, 2013, [Peer-reviewed], [Lead author, Last author, Corresponding author], [Domestic magazines]
    English, Scientific journal
  • The effects of heat induction and the siRNA biogenesis pathway on the transgenerational transposition of ONSEN, a copia-like retrotransposon in Arabidopsis thaliana.
    Wataru Matsunaga; Akie Kobayashi; Atsushi Kato; Hidetaka Ito
    Plant & cell physiology, 53, 5, 824, 33, May 2012, [Peer-reviewed], [Last author, Corresponding author], [Domestic magazines]
    English, Scientific journal
  • Small RNAs and transposon silencing in plants.
    Hidetaka Ito
    Development, growth & differentiation, 54, 1, 100, 7, Jan. 2012, [Peer-reviewed], [Invited], [Lead author, Last author, Corresponding author], [Domestic magazines]
    English, Scientific journal
  • An siRNA pathway prevents transgenerational retrotransposition in plants subjected to stress.
    Hidetaka Ito; Hervé Gaubert; Etienne Bucher; Marie Mirouze; Isabelle Vaillant; Jerzy Paszkowski
    Nature, 472, 7341, 115, 9, 07 Apr. 2011, [Peer-reviewed], [Lead author], [International Magazine]
    English, Scientific journal
  • Ecotype-specific and chromosome-specific expansion of variant centromeric satellites in Arabidopsis thaliana.
    Hidetaka Ito; Asuka Miura; Kazuya Takashima; Tetsuji Kakutani
    Molecular genetics and genomics : MGG, 277, 1, 23, 30, Jan. 2007, [Peer-reviewed], [Lead author], [International Magazine]
    English, Scientific journal
  • Genomic and genetic characterization of rice Cen3 reveals extensive transcription and evolutionary implications of a complex centromere.
    Huihuang Yan; Hidetaka Ito; Kan Nobuta; Shu Ouyang; Weiwei Jin; Shulan Tian; Cheng Lu; R C Venu; Guo-Liang Wang; Pamela J Green; Rod A Wing; C Robin Buell; Blake C Meyers; Jiming Jiang
    The Plant cell, 18, 9, 2123, 33, Sep. 2006, [Peer-reviewed], [Lead author], [International Magazine]
    English, Scientific journal
  • A direct repeat sequence associated with the centromeric retrotransposons in wheat.
    Hidetaka Ito; Shuhei Nasuda; Takashi R Endo
    Genome, 47, 4, 747, 56, Aug. 2004, [Peer-reviewed], [Lead author], [International Magazine]
    English, Scientific journal
  • The genome sequence and structure of rice chromosome 1.
    Takuji Sasaki; Takashi Matsumoto; Kimiko Yamamoto; Katsumi Sakata; Tomoya Baba; Yuichi Katayose; Jianzhong Wu; Yoshihito Niimura; Zhukuan Cheng; Yoshiaki Nagamura; Baltazar A Antonio; Hiroyuki Kanamori; Satomi Hosokawa; Masatoshi Masukawa; Koji Arikawa; Yoshino Chiden; Mika Hayashi; Masako Okamoto; Tsuyu Ando; Hiroyoshi Aoki; Kohei Arita; Masao Hamada; Chizuko Harada; Saori Hijishita; Mikiko Honda; Yoko Ichikawa; Atsuko Idonuma; Masumi Iijima; Michiko Ikeda; Maiko Ikeno; Sachie Ito; Tomoko Ito; Yuichi Ito; Yukiyo Ito; Aki Iwabuchi; Kozue Kamiya; Wataru Karasawa; Satoshi Katagiri; Ari Kikuta; Noriko Kobayashi; Izumi Kono; Kayo Machita; Tomoko Maehara; Hiroshi Mizuno; Tatsumi Mizubayashi; Yoshiyuki Mukai; Hideki Nagasaki; Marina Nakashima; Yuko Nakama; Yumi Nakamichi; Mari Nakamura; Nobukazu Namiki; Manami Negishi; Isamu Ohta; Nozomi Ono; Shoko Saji; Kumiko Sakai; Michie Shibata; Takanori Shimokawa; Ayahiko Shomura; Jianyu Song; Yuka Takazaki; Kimihiro Terasawa; Kumiko Tsuji; Kazunori Waki; Harumi Yamagata; Hiroko Yamane; Shoji Yoshiki; Rie Yoshihara; Kazuko Yukawa; Huisun Zhong; Hisakazu Iwama; Toshinori Endo; Hidetaka Ito; Jang Ho Hahn; Ho-Il Kim; Moo-Young Eun; Masahiro Yano; Jiming Jiang; Takashi Gojobori
    Nature, 420, 6913, 312, 6, 21 Nov. 2002, [Peer-reviewed], [International Magazine]
    English, Scientific journal
■ Other Activities and Achievements
■ Books and other publications
  • Biological Sciences Genetics
    Tetsuji Kakutani; Hidetaka Ito, Transposons: Genes Moving Through the Genome - Drivers of Genome Evolution
    Jul. 2017, 9784860434861, Japanese, [Contributor]
  • The Ecology of Epigenetics
    Hidetaka Ito, Ecology of Epigenetics: How genes are regulated in response to the environment
    Bunichi Sogo Shuppan, Feb. 2017, 9784829962077, 250, Japanese, Scholarly book, [Joint work]
  • Transgenerational Epigenetics
    ITO Hidetaka, Plant Models of Transgenerational Epigenetic Inheritance.
    Elsevier, Jun. 2014, 9780124059443, 147-161, English, Scholarly book, [Contributor]
  • Chemistry & Biology
    Hidetaka Ito, Environmental stress and genome evolution
    Sep. 2013, Japanese, [Contributor]
  • RNA to integrate biomolecules
    Hidetaka Ito, Small RNAs and intergenerational signals
    YODOSHYA, May 2013, [Contributor]
■ Lectures, oral presentations, etc.
  • シロイヌナズナ自然系統における揮発性有機化合物応答と耐熱性の多様性
    Siru Chen; Baibhav R. Barbaruah; Hidetaka Ito
    日本遺伝学会第98回大会, 09 Sep. 2026, English, Oral presentation
    09 Sep. 2026 - 11 Sep. 2026, 50683450
  • Volatile Organic Compounds Prime Heat Tolerance Through Chromatin-Dependent Heat Shock Pathways in Arabidopsis
    Baibhav R. Barbaruah; Shuo Feng; Hidetaka Ito
    日本遺伝学会第98回大会, 09 Sep. 2026, English, Oral presentation
    09 Sep. 2026 - 11 Sep. 2026, 50683450
  • Ecotype-Specific Sensitivity to DNA Methylation Inhibitors in Arabidopsis cmt3 Mutants
    Sun Xin; Guo Du; Atsushi Kato; Hidetaka Ito
    日本遺伝学会第98回大会, 09 Sep. 2026, English, Oral presentation
    09 Sep. 2026 - 11 Sep. 2026
  • Stress-Induced ONSEN Mobilization Generates an Early-Flowering nrpd1 Mutant
    Sari Saren, Hiroki Takagi, Kaisei Miyaki, Hidetaka Ito
    日本遺伝学会第98回大会, 09 Sep. 2026, English, Oral presentation
    09 Sep. 2026 - 11 Sep. 2026
  • プラズマ処理が誘導する植物のストレス応答とエピジェネティック制御
    伊藤 秀臣
    日本植物学会第90回大会, 04 Sep. 2026, 公益社団法人 日本植物学会, Japanese, Public symposium
    04 Sep. 2026 - 06 Sep. 2026, 東京理科大学 野田キャンパス（千葉県野田市）, Japan, 50082751, [Invited], [Domestic Conference]
  • 北海道胆振東部地震における北海道大学の対応と今後の課題
    伊藤 秀臣
    日本遺伝学会第97回大会, 11 Sep. 2025, 日本遺伝学会, Japanese, Invited oral presentation
    10 Sep. 2025 - 12 Sep. 2025, 神戸大学, Japan, [Invited], [Domestic Conference]
  • The Peroxisomal Membrane Protein RHE1 is a Key Regulator of ROS-Dependent Hypocotyl Elongation in Arabidopsis
    Lin Yibin; Hidetaka Ito
    日本遺伝学会第97回大会, 11 Sep. 2025, 日本遺伝学会, English, Oral presentation
    10 Sep. 2025 - 12 Sep. 2025, 神戸大学, Japan, [Domestic Conference]
  • Decoding plant stress communication: Volatile Organic Compound-Induced HsfA2Expression and Stress Tolerance in plants
    Baibhav Raj Barbaruah; Hidetaka Ito
    日本遺伝学会第97回大会, 11 Sep. 2025, 日本遺伝学会, English, Oral presentation
    10 Sep. 2025 - 12 Sep. 2025, 神戸大学, Japan, 50683450, [Domestic Conference]
  • Physiological and Molecular Responses to Drought in Four Japanese Capsicum annuum Varieties
    Rahmadani Primanindita AIRLANGGA; Yoko IKEDA; Takakazu MATSUURA; Diana Mihaela BUZAS; Hidetaka ITO
    日本遺伝学会第97回大会, 10 Sep. 2025, 日本遺伝学会, English, Oral presentation
    10 Sep. 2025 - 12 Sep. 2025, 神戸大学, Japan, [Domestic Conference]
  • Investigating the Impact of Transposon Insertion on Flowering Time Regulatory Genes
    Sari Saren; Hidetaka Ito
    日本遺伝学会第97回大会, 10 Sep. 2025, 日本遺伝学会, English, Oral presentation
    10 Sep. 2025 - 12 Sep. 2025, 神戸大学, Japan, [Domestic Conference]
  • Investigation on Drought Stress Effect on Four Japanese Chili Pepper Varieties
    Rahmadani Primanindita AIRLANGGA; Hidetaka ITO
    日本育種学会 第147回講演会, 21 Mar. 2025, 日本育種学会, English, Oral presentation
    20 Mar. 2025 - 21 Mar. 2025, 仙台, Japan, [Domestic Conference]
  • Decoding Plant Stress Communication: VOC-Induced HsfA2 Expression and Heat Stress Tolerance in Arabidopsis thaliana
    Baibhav Raj Barbaruah; Feng Shuo; Hidetaka Ito
    日本育種学会 第147回講演会, 21 Mar. 2025, 日本育種学会, English, Oral presentation
    20 Mar. 2025 - 21 Mar. 2025, 仙台, Japan, 50683450, [Domestic Conference]
  • 環境ストレス下でのトランスポゾン活性化とその 育種応用の可能性
    伊藤 秀臣
    日本育種学会 第147回講演会, 20 Mar. 2025, 日本育種学会, Japanese, Oral presentation
    20 Mar. 2025 - 21 Mar. 2025, 仙台, Japan, 45565353, [Domestic Conference]
  • Investigating the Impact of Transposon Insertion on Flowering Time Regulatory Genes
    Sari Saren; Hidetaka Ito
    The 47th Annual Meeting of the Molecular Biology Society of Japan 主催, 27 Nov. 2024, 日本分子生物学会, Poster presentation
    27 Nov. 2024 - 29 Nov. 2024
  • Response and Challenges at Hokkaido University during the Eastern Iburi Earthquake and Subsequent Blackout
    Hidetaka Ito
    日本育種学会第146回講演会, 19 Sep. 2024, 日本育種学会, Public symposium
    19 Sep. 2024 - 20 Sep. 2024, 広島大学, Japan, [Invited], [Domestic Conference]
  • The transposition of a heat-activated retrotransposon ONSEN resulted in changes in the hypocotyl elongation
    Ryu Hasegawa; Hidetaka Ito
    第65回日本植物生理学会年会, 18 Mar. 2024, 日本植物生理学会, Poster presentation
    神戸国際会議場, [Domestic Conference]
  • The transpositional activity of ONSEN transposons varies among different mutants in the RdDM-related pathways
    SUN XINGYU; Yui Hayshi; Hidetaka.Ito
    日本遺伝学会第95回大会, 07 Sep. 2023, 日本遺伝学会, Oral presentation
    熊本, [Domestic Conference]
  • シロイヌナズナにおける熱活性型レトロトランスポゾンの制御機構の解明
    牛小蛍; 伊藤秀臣
    日本遺伝学会第95回大会, 07 Sep. 2023, 日本遺伝学会, Oral presentation
    [Invited], [Domestic Conference]
  • Exploration of the association between HSFA2 and ONSEN expression
    Zhiyu Ge; Xiaoying Niu; Hidetaka Ito
    日本遺伝学会第95回大会, 07 Sep. 2023, 日本遺伝学会, Oral presentation
    [Domestic Conference]
  • DNA methylation mediates transcriptional regulation of heat-active retrotransposons by HDAC
    SUN XIN; Hidetaka Ito
    日本遺伝学会第95回大会, 06 Sep. 2023, 日本遺伝学会, Oral presentation
    [Domestic Conference]
  • Novel mechanism of transposon repression by histone deacetylases.
    Ito, H; Niu, X; Ikeda, Y; Saze, H; Kanehira, R; Son, N
    The 33rd International Conference on Arabidopsis Research, 07 Jun. 2023, English, Nominated symposium
    05 Jun. 2023 - 09 Jun. 2023, 幕張メッセ, Japan, [Invited], [International presentation]
  • Regulatory mechanism of heat-active retrotransposons by DDR complex
    Xiaoyin Niu; Lu Chen; Atsushi Kato; Hidetaka Ito
    日本遺伝学会第94回大会, 14 Sep. 2022, 日本遺伝学会, Oral presentation
    北海道大学, Japan, [Domestic Conference]
  • Studies on environmental stress-activated transposons
    Hidetaka Ito
    2021年度日本遺伝学会奨励賞受賞講演, 10 Sep. 2021, 日本遺伝学会, Japanese, Invited oral presentation
    08 Sep. 2021 - 10 Sep. 2021, オンライン, Japan, [Invited], [Domestic Conference]
  • Investigating a novel transposable regulator of heat-responsive retrotransposons “ONSEN” in Arabidopsis thaliana.
    SYOUEI GYUU; KANAKO TAKEHIRA; ATUSHI KATO; HIDETAKA ITO
    日本遺伝学会第93回大会, 08 Sep. 2021, 日本遺伝学会, Japanese, Oral presentation
    08 Sep. 2021 - 10 Sep. 2021, オンライン, Japan, [Domestic Conference]
  • Analysis of transcriptional regulation of heat-activated retrotransposons in Arabidopsis thaliana
    Xiaoying Niu; Lu Chen; Atsushi Kato; Hidetaka Ito
    IPSR_Web_Forum_2021, 07 Sep. 2021, The IPSR International Plant Web Forum Organizing Committee, English, Poster presentation
    06 Sep. 2021 - 07 Sep. 2021, Japan, [International presentation]
  • Regulation of transposons in stem apical stem cells
    Hidetaka Ito
    The 84th Congress of the Botanical Society of Japan, 19 Sep. 2020, 日本植物学会, Japanese, Public symposium
    オンライン, [Invited], [Domestic Conference]
  • Survival strategies of selfish gene transposons and host plants
    Hidetaka Ito
    北海道植物学会講演会, 18 Dec. 2019, 北海道植物学会, Japanese, Public discourse
    18 Dec. 2019, Hokkaido University, Japan, [Invited], [Domestic Conference]
  • The effect of Zebularine for ONSEN activation in Adzuki upon heat stress
    Patwira Boonjing; Yukari Masuta; Hidetaka Ito
    PGRP 2019 Nara, 18 Mar. 2019, English, Poster presentation
    東大寺総合文化センター, [International presentation]
  • A novel regulation of transposon expression via DNA methylation
    Masataka Yamada; Kosuke Nozawa; Atsushi Kato; Hidetaka Ito
    The Japanese society of plant physiologists, 13 Mar. 2019, The Japanese society of plant physiologists, Japanese, Oral presentation
    Nagoya University, [Domestic Conference]
  • A novel regulation of transposon via DNA methylation
    Masahiro Fukuda; Kosuke Nozawa; Kato Atsushi; Hidetaka Ito
    The Japanese society of plant physiologists, 13 Mar. 2019, The Japanese society of plant physiologists, Japanese, Poster presentation
    Nagoya University, [Domestic Conference]
  • The Potential for ONSEN Transposition in Arabidopsis thaliana
    Yuto Takai; Tetsu Iwase; Yukari Masuta; Atsushi Kato; Hidetaka Ito
    The 90th Annual Meeting of the Genetics Society of Japan, 19 Sep. 2018, 日本遺伝学会, Japanese, Oral presentation
    奈良先端科学技術大学院大学, [Domestic Conference]
  • Analysis of Pol IV-specific transposition regulation of heat-activated retrotransposons
    Kanako Takehira; Yui Hayashi; Kosuke Nozawa; Atsushi Kato; Hidetaka Ito
    The 90th Annual Meeting of the Genetics Society of Japan, 19 Sep. 2018, 日本遺伝学会, Japanese, Oral presentation
    奈良先端科学技術大学院大学, [Domestic Conference]
  • Functional characterization of ONSEN activated by heat stress in transgenic rice
    Patwira Boonjing; Yoshiki Habu; Hiroyuki Tsuji; Yukari Masuta; Atsushi Kato; Hidetaka Ito
    日本遺伝学会第90回大会, 19 Sep. 2018, 日本遺伝学会, English, Oral presentation
    奈良先端科学技術大学院大学, [Domestic Conference]
  • Adaptive evolution of environmental stress-active retroposon and health science
    Hidetaka Ito
    The 13th Annual Meeting of the Japanese Society for Food Immunology, 09 Nov. 2017, 日本食品免疫学会, Japanese, Invited oral presentation
    東京大学伊藤国際学術研究センター, [Invited], [Domestic Conference]
  • Environmental stress-activated retrotransposons and adaptive evolution
    Hidetaka Ito
    Jointly sponsored by the International Research Unit for Frontier Sciences, Kyoto University Interdisciplinary Research Meeting "Living Body, Human Body, Spirit, and Universe: Exploring the Deeper Layers of Connection, 03 May 2017, 京都大学基礎物理学研究所, Japanese, Invited oral presentation
    02 May 2017 - 03 May 2017, 京都大学 基礎物理学研究所 湯川記念館 パナソニック国際交流ホール, Japan, [Invited], [Domestic Conference]
  • Environmental stress-responsive retrotransposons and adaptive evolution in plants
    Hidetaka Ito
    Nichiyaku Retroposon Study Group, 25 Mar. 2017, Japanese, Invited oral presentation
    日本薬科大学お茶の水キャンパス, [Invited], [Domestic Conference]
  • Transposons create environmental stress tolerant plants.
    ITO Hidetaka
    第58回日本植物生理学会, 16 Mar. 2017, English, Nominated symposium
    鹿児島大学, [Invited], [Domestic Conference]
  • Adaptation and regulation of a stress activated transposon in plant
    ITO Hidetaka
    International Symposium on Environmental Stress Adaptation & Memory in Plants, 27 Feb. 2017, English, Nominated symposium
    RIKEN Yokohama Campus, [Invited], [International presentation]
  • Epigenetic regulation of transposons in plants
    Hidetaka Ito
    The 1st Study meeting on Endogenous Virus-like Elements, 16 Dec. 2016, ENDEAVR若手内在性ウィルス様エレメント研究会, Japanese, Invited oral presentation
    京都大学国際科学イノベーション棟, [Invited], [Domestic Conference]
  • Analysis of transcriptional regulation of a heat-activated retrotransposon in Arabidopsis
    Kosuke Nozawa; Atsushi Kato; Hidetaka Ito
    Cold Spring Harbor Asia conferences, 18 Nov. 2016, English, Poster presentation
    Awaji Yumebutai Conference Center, Japan, [International presentation]
  • Analysis and application of heat-activated retrotransposon in Brassicaceae
    ITO Hidetaka
    日本育種学会第130回講演会, 24 Sep. 2016, 日本育種学会, Japanese, Nominated symposium
    鳥取市, [Invited], [Domestic Conference]
  • Analysis of transcriptional regulation of heat-activated retrotransposons
    Kosuke Nozawa; Seiji Masuda; Atsushi Kato; Hidetaka Ito
    The 88th Annual Meeting of the Genetics Society of Japan, 08 Sep. 2016, 日本遺伝学会, Japanese, Oral presentation
    三島市, [Domestic Conference]
  • A transpositional regulation of a heat-activated retrotransposon in Arabidopsis.
    Yui Hayashi; Yukari Masuta; Atsushi Kato; Hidetaka Ito
    日本遺伝学会第88回大会, 08 Sep. 2016, 日本遺伝学会, Japanese, Oral presentation
    三島市, [Domestic Conference]
  • pigenetic regulation of stress-responsible transposon in plants
    ITO Hidetaka
    MB2015, 02 Dec. 2015, BMB, Japanese, Invited oral presentation
    神戸ポートアイランド, [Domestic Conference]
  • Analysis of transcriptional regulation of heat-activated retrotransposon in ecotypes of Arabidopsis thaliana.
    野沢 紘佑; 升田 誠二; 加藤 敦之; 伊藤 秀臣
    日本遺伝学会第87回大会, 26 Sep. 2015, 日本遺伝学会, Japanese, Oral presentation
    東北大学, [Domestic Conference]
  • Epigenetic regulation of a heat-activated transposon in plant
    ITO Hidetaka
    The 56th Annual Meeting of The Japanese Society of Plant Physiologists, 16 Mar. 2015, The Japanese Society of Plant Physiologists, English, Nominated symposium
    Tokyo University of Agriculture, [Invited], [Domestic Conference]
  • Transposable elements and host response to environmental stress
    Hidetaka Ito
    Research Meeting of the Institute of Genetics, 27 Feb. 2015, Japanese, Invited oral presentation
    国立遺伝学研究所, [Invited], [Domestic Conference]
  • Epigenetic regulation of a stress activated transposon in plant
    Hidetaka Ito; Jong-Myong Kim; Wataru Matsunaga; Akihiro Matsui; Tetsuro Toyoda; Takaho A. Endo; Junko Ishida; Taeko Morosawa; Motoaki Seki
    第5回NIBB-MPIPZ-TLL合同シンポジウム, 24 Nov. 2014, English, Poster presentation
    Max Planck Institute for Plant Breeding Research, MPIPZ, [International presentation]
  • Search for heat-activated retrotransposons in radish
    Yuki Kawagishi; Yukari Masuta; Seiji Masuda; Wataru Matsunaga; Atsushi Kato; Hidetaka Ito
    The 86th Annual Meeting of the Genetics Society of Japan, 17 Sep. 2014, Japanese, Oral presentation
    [Domestic Conference]
  • Transcriptional Activity and Silencing of Heat-activated Retrotransposons in Arabidopsis thaliana
    Wataru Matsunaga; Yukari Masuta; Namiki Mitani; Jian Feng Ma; Atsushi Kato; Hidetaka Ito
    The 86th Annual Meeting of the Genetics Society of Japan, 17 Sep. 2014, Japanese, Oral presentation
    [Domestic Conference]
  • Analysis of heat-activated transposons in Brassica napus
    Miu Sato; Seiji Masuda; Yukari Masuta; Atsushi Kato; Hidetaka Ito
    The 86th Annual Meeting of the Genetics Society of Japan, 17 Sep. 2014, Japanese, Oral presentation
    [Domestic Conference]
  • Role of transposons in environmental stress response
    Hidetaka Ito
    Japan Laboratory Animal Science and Technology 2014, 15 May 2014, 日本実験動物学会, Japanese, Invited oral presentation
    札幌コンベンションセンター, [Invited], [Domestic Conference]
  • Environmental response and epigenetic regulation of transposable elements in plants.
    Hidetaka Ito
    The 55th Annual Meeting of the Japanese Society of Plant Physiologists Symposium, 19 Mar. 2014, 日本植物生理学会, English, Nominated symposium
    富山大学, [Invited], [Domestic Conference]
  • Genome reconstruction and a heat-induced retrotransposon in Brassica
    Hidetaka Ito; Yukari Masuta; Wataru Matsunaga; Seiji Masuda; Atsushi Kato
    第124回育種学会講演会, 12 Oct. 2013, 日本育種学会, Japanese, Oral presentation
    鹿児島大学, [Domestic Conference]
  • Plant siRNA and transgenerational inheritance
    Hidetaka Ito
    Research Meeting of the Institute of Genetics, 10 Oct. 2013, Japanese, Invited oral presentation
    国立遺伝学研究所, [Invited], [Domestic Conference]
  • Search for heat-activated retrotransposons with the potential for transposition
    Seiji Masuda; Yukari Masuta; Atsushi Kato; Hidetaka Ito
    The 85th Annual Meeting of the Genetics Society of Japan, 19 Sep. 2013, 日本遺伝学会, Japanese, Oral presentation
    慶応大学日吉キャンパス, [Domestic Conference]
  • Tissue-specific regulation of heat-activated retrotransposons in Arabidopsis thaliana
    Wataru Matsunaga; Yukari Masuta; Atsushi Kato; hidetaka Ito
    The 85th Annual Meeting of the Genetics Society of Japan, 19 Sep. 2013, 日本遺伝学会, Japanese, Oral presentation
    慶応大学日吉キャンパス, [Domestic Conference]
  • Analysis of the regulatory mechanism of Arabidopsis retrotransposon AtRE1 expression
    Yumi Yamagishi; Atsushi Kato; Hidetaka Ito
    The 85th Annual Meeting of the Genetics Society of Japan, 19 Sep. 2013, 日本遺伝学会, Japanese, Oral presentation
    慶応大学日吉キャンパス, [Domestic Conference]
  • A nature of a stress-activated transposon and the effect on the host genome
    Hidetaka Ito; Jong-Myong KIM; Wataru Matsunaga; Tetsuro Toyoda; Takaho A. Endo; Junko Ishida; Taeko Morosawa; Motoaki Seki
    ICAR2013, 25 Jun. 2013, English, Poster presentation
    SYDNEY, [International presentation]
  • A heat-induced activation and epigenetic regulation of a plant mobile element
    伊藤 秀臣
    CMCB2013, 15 Jun. 2013, English, Invited oral presentation
    SUZHOU, [International presentation]
  • An siRNA pathway controls heat-activated retrotransposition in plants
    Hidetaka Ito
    Plant Electron Microscopy Workshop for Young Scientists 2012, 15 Jan. 2013, 豊岡 公徳 (理研 植物センター) 佐藤 繭子 (理研 植物センター) 永田 典子 (日本女子大 バイオイメージングセンター), Japanese, Poster presentation
    理化学研究所 横浜研究所 交流棟ホール, [Domestic Conference]
  • Transposon silencing and genome evolution through an siRNA
    ITO Hidetaka
    第３５回日本分子生物学会年会, 14 Dec. 2012, 日本分子生物学会, Japanese, Nominated symposium
    福岡国際会議場・マリンメッセ福岡, [Invited], [Domestic Conference]
  • Environmental stress and genome evolution: High-temperature stress-activated transposons and host defense
    Hidetaka Ito
    The 44th Symposium on Species Biology, 09 Dec. 2012, 種生物学会, Japanese, Nominated symposium
    奥琵琶湖マキノパークホテル＆セミナーハウス, [Invited], [Domestic Conference]
  • A small RNA regulates a heat-activated transposon
    ITO Hidetaka
    Frontiers in Plant RNA Research 2012, 17 Oct. 2012, Hokkaido University Leader Development System in the Basic Interdisciplinary Research Area and RIKEN Biomass Engineering Program, English, Invited oral presentation
    The Conference Hall, Hokkaido Univ., Sapporo Campus, [Invited], [International presentation]
  • Regulation of Arabidopsis heat-activated retrotransposon transposition
    Wataru Matsunaga; Yukari Masuta; Atsushi Kato; Hidetaka Ito
    The 84th Annual Meeting of the Genetics Society of Japan, 26 Sep. 2012, 日本遺伝学会, Japanese, Oral presentation
    九州大学医学部 百年講堂・同窓会館, [Domestic Conference]
  • Genomic impact of a stress-induced retrotransposon
    ITO Hidetaka
    63rd FUJIHARA SEMINAR 2012, 02 Aug. 2012, Norihiro OKADA (Tokyo Institute of Technology), English, Invited oral presentation
    芝蘭会館, [Invited], [International presentation]
  • The Effects of a Stress-activated Transposon on Arabidopsis Genome
    ITO Hidetaka
    The 8th Okazaki Biology Conference, 22 Mar. 2012, Aya Takahashi (National Institute of Genetics, Japan) Fumitoshi Ishino (Tokyo Medical and Dental University, Japan) Michael Purugganan (New York University, USA) Mitsuyasu Hasebe (National Institute for Basic Biology, Japan), English, Invited oral presentation
    [Invited], [International presentation]
  • A stress-induced activation and epigenetic regulation of a retrotransposon
    Hidetaka Ito
    Symposium at the 53rd Annual Meeting of the Japanese Society of Plant Physiologists, 05 Mar. 2012, 日本植物生理学会, English, Invited oral presentation
    京都産業大学, [Invited], [Domestic Conference]
  • Transgenerational effects and genomic impacts in environmental stress
    ITO Hidetaka
    3rd International Conference and Workshop, 26 Feb. 2012, English, Oral presentation
    [International presentation]
  • Transgenerational effect and epigenetic control in environmental regulation
    Hidetaka Ito
    The 14th Seoul National University - Hokkaido University Joint Symposium, Nov. 2011, English, Oral presentation
    [International presentation]
  • Stress-induced transposons and genome evolution
    Hidetaka Ito
    The 83rd Annual Meeting of the Genetics Society of Japan, Sep. 2011, 日本遺伝学会, Japanese, Nominated symposium
    京都大学農学部・農学研究科（吉田キャンパス北部構内）, [Domestic Conference]
  • An siRNA pathway controls retrotransposition in plants subjected to stress
    伊藤 秀臣
    22nd ICAR, Jun. 2011, English, Poster presentation
    [International presentation]
  • Stress-induced retrotransposition controlled by siRNA pathway in Arabidopsis
    The 13th Seoul National University - Hokkaido University Joint Symposium, Nov. 2010
  • Regulation of transgenerational transposition of heat-activated retrotransposons in Arabidopsis thaliana by siRNA
    Hidetaka Ito
    The 82th Annual Meeting of the Genetics Society of Japan, Sep. 2010, 日本遺伝学会, Japanese, Oral presentation
    北海道大学高等教育機能開発総合センターS棟および大講堂, [Domestic Conference]
■ Syllabus
  • 環境応答システム科学特論, 2024年, 修士課程, 生命科学院
  • 大学院共通授業科目（一般科目）：複合領域, 2024年, 修士課程, 大学院共通科目
  • 大学院共通授業科目（教育プログラム）：新渡戸カレッジオナーズプログラム大学院カリキュラム, 2024年, 修士課程, 大学院共通科目
  • 大学院共通授業科目（教育プログラム）：新渡戸カレッジオナーズプログラム大学院カリキュラム, 2024年, 修士課程, 大学院共通科目
  • 大学院共通授業科目（一般科目）：複合領域, 2024年, 修士課程, 大学院共通科目
  • 生命システム科学基礎論, 2024年, 修士課程, 生命科学院
  • 生物学Ⅰ, 2024年, 学士課程, 全学教育
  • 細胞生物学概論, 2024年, 学士課程, 理学部
  • 科学・技術の世界（1単位）, 2024年, 学士課程, 全学教育
  • ISP生物科学実習Ⅱ・a, 2024年, 学士課程, 理学部
  • ISP生物科学実習Ⅱ・b, 2024年, 学士課程, 理学部
  • 形態機能学実習, 2024年, 学士課程, 理学部
  • 形態機能学実習, 2024年, 学士課程, 理学部
  • 形態機能学Ⅰ, 2024年, 学士課程, 理学部
■ Affiliated academic society
  • Present
    Japanese Society of Plant Physiology
  • Present
    Japanese Society of Genetics
  • Present
    日本育種学会
■ Research Themes
  • 植物間コミュニケーションが導く環境適応進化の解明
    科学研究費助成事業
    27 Jun. 2025 - 31 Mar. 2028
    伊藤 秀臣
    日本学術振興会, 挑戦的研究(萌芽), 北海道大学, 25K22430
  • プラズマ技術を用いたシロイヌナズナの発芽とストレス応答のエピジェネティクス制御
    科学研究費助成事業
    01 Apr. 2025 - 31 Mar. 2027
    伊藤 秀臣
    日本学術振興会, 学術変革領域研究(A), 北海道大学, 25H02203
  • Epigenetic Regulation of Japanese Pepper Varieties under Drought Stress
    令和8年度国立大学法人筑波大学つくば機能植物イノベーション 研究センター「形質転換植物デザイン研究拠点」共同利用・共同 研究課題
    Apr. 2026 - Mar. 2027
    筑波大学つくば機能植物イノベーション 研究センター, 筑波大学つくば機能植物イノベーション 研究センター, Principal investigator
  • 植物種子超低温保存におけるエピジェネティック修飾動態解析
    2026年度基礎生物学研究所 共同利用研究 生物遺伝資源新規保存技術開発共同利用研究 【定時】
    Apr. 2026 - Mar. 2027
    基礎生物学研究所, 基礎生物学研究所, Principal investigator
  • カブ (Brassica rapa) における光『非』依存的なアントシアニン蓄積機構の解明
    科学研究費助成事業
    01 Apr. 2023 - 31 Mar. 2026
    高木 宏樹; 西内 巧; 殿崎 薫; 伊藤 秀臣
    日本学術振興会, 基盤研究(C), 石川県立大学, 23K05170
  • Analysis of epigenetic modification dynamics in ultra-low temperature storage of plant seeds
    2025年度基礎生物学研究所 共同利用研究
    Apr. 2025 - Mar. 2026
    伊藤秀臣
    基礎生物学研究所, 生物遺伝資源新規保存技術開発共同利用研究 【定時】, 基礎生物学研究所, Principal investigator
  • Epigenetic Regulation of Japanese Pepper Varieties under Drought Stress
    令和7年度国立大学法人筑波大学つくば機能植物イノベーション 研究センター「形質転換植物デザイン研究拠点」共同利用・共同 研究課題
    Apr. 2025 - Mar. 2026
    Hidetaka Ito; Diana Mihaela Buzas; RAHMADANI P. AIRLANGGA
    筑波大学つくば機能植物イノベーション 研究センター, 筑波大学つくば機能植物イノベーション 研究センター, Principal investigator
  • 植物種子超低温保存におけるエピジェネティック修飾動態解析
    ２０２４年度基礎生物学研究所 共同利用研究
    Apr. 2024 - Mar. 2025
    伊藤秀臣
    基礎生物学研究所, 生物遺伝資源新規保存技術開発共同利用研究 【定時】, 基礎生物学研究所, Principal investigator, Competitive research funding
  • トランスポゾンを利用したダイコンの変異体ライブラリーの整備
    令和6年度 研究開発助成事業
    Apr. 2024 - Mar. 2025
    Hidetaka Ito; Hiroki Takagi
    公益財団法人G-7奨学財団, Principal investigator, Competitive research funding
  • Epigenetic Regulation of Japanese Pepper Varieties under Drought Stress
    令和6年度国立大学法人筑波大学つくば機能植物イノベーション 研究センター「形質転換植物デザイン研究拠点」共同利用・共同 研究課題
    Apr. 2024 - Mar. 2025
    Hidetaka Ito; Diana Mihaela Buzas; RAHMADANI P. AIRLANGGA
    筑波大学つくば機能植物イノベーション 研究センター, 筑波大学つくば機能植物イノベーション 研究センター, Principal investigator
  • 新規トランスポゾン転移制御機構の解明
    科学研究費助成事業 基盤研究(C)
    01 Apr. 2021 - 31 Mar. 2024
    伊藤 秀臣
    日本学術振興会, 基盤研究(C), 北海道大学, 21K06008
  • Epigenetic Regulation of Japanese Pepper Varieties under Drought Stress
    令和５年度国立大学法人筑波大学つくば機能植物イノベーション 研究センター「形質転換植物デザイン研究拠点」共同利用・共同 研究課題
    Apr. 2023 - Mar. 2024
    Hidetaka Ito,Diana Mihaela Buzas,RAHMADANI P. AIRLANGGA
    筑波大学つくば機能植物イノベーション 研究センター, 筑波大学つくば機能植物イノベーション 研究センター, Principal investigator
  • 内在性トランスポゾンを利用したダイズのゲノム育種
    令和５年度 コンソーシアム形成型「ロバスト農林水産工学研究プログラム」
    Apr. 2023 - Mar. 2024
    Hidetaka Ito, Akira Kanazawa
    国立大学法人 北海道大学 ロバスト農林水産工学国際連携研究教育拠点 「知」の集積と活用の場 産学官連携協議会 ロバスト農林水産工学研究開発プラットフォーム, Principal investigator, Competitive research funding
  • トランスポゾンを利用したダイコンの変異体ライブラリーの整備
    令和５年度 研究開発助成事業
    Apr. 2023 - Mar. 2024
    Hidetaka Ito, Hiroki Takagi
    公益財団法人G-7奨学財団, Principal investigator, Competitive research funding
  • Discovery of novel regulators of " TRANSPOSONS ", the mobile genes that drive evolution.
    EXPLORATION FRANCE
    Jun. 2023 - Jun. 2023
    Hidetaka Ito
    French Embassy, Principal investigator, ［Internationally co-authored］
  • ヒストン脱アセチル化酵素による新規トランスポゾン抑制機構の解明
    令和4年度 岡山大学資源植物科学研究所 共同研究
    Apr. 2022 - Mar. 2023
    伊藤秀臣、池田陽子、牛小蛍
    岡山大学資源植物科学研究所, 岡山大学資源植物科学研究所, Principal investigator, Competitive research funding, R406
  • ヒストン脱アセチル化酵素による新規トランスポゾン抑制機構の解明
    令和3年度 岡山大学資源植物科学研究所 共同研究
    Apr. 2021 - Mar. 2022
    伊藤 秀臣; 池田 陽子、牛小蛍
    岡山大学資源植物科学研究所, 岡山大学資源植物科学研究所, Principal investigator, R305
  • Analysis of a novel transposon regulatory mechanism depending on DNA demethylation
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    01 Apr. 2018 - 31 Mar. 2021
    Ito Hidetaka
    In Arabidopsis, a mutant of CMT3, a DNA methyltransferase responsible for the methylation of CHG sequences, reduced the expression of high-temperature activated transposons (TEs). This phenomenon suggests the existence of a regulatory mechanism that is different from the previously considered regulation of TEs by DNA methylation and repressive histone modifications. The transcriptional activation of TE is dependent on CMT3, and the loss-of-function mutation of CMT3 increases CHH methylation, which is mediated by CMT2. Furthermore, it was found that CMT3 inhibits the binding of CMT2 on the TE sequence, thereby suppressing CHH methylation and promoting TE transcription.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 18K06050
  • 植物における新規環境ストレス応答因子の解明
    2020年度国立遺伝学研究所共同研究（Ｂ）
    Apr. 2020 - Mar. 2021
    伊藤 秀臣; 角谷 徹仁
    国立遺伝学研究所, 国立遺伝学研究所, Principal investigator, Competitive research funding
  • Identification and functional analysis of novel transposition regulators of transposons
    Grants-in-Aid for Scientific Research
    2021 - 2021
    伊藤 秀臣
    Japan Society for the Promotion of Science, Fund for the Promotion of Joint International Research (Fostering Joint International Research (A)), Hokkaido University, 21KK0263
  • Spatiotemporal responses and molecular mechanisms of memory in response to drought or temperature stress in plants
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
    29 Jun. 2015 - 31 Mar. 2020
    Yamaguchi-Shinozaki Kazuko
    This study aimed to clarify the molecular mechanisms of plant responses under drought and temperature stress conditions and the mechanisms of plant long-term memory in response to stress. We elucidated that plant subclass I SnRK2 kinases activated by osmotic stress regulate mRNA degradation under the stress conditions, and further identified three RAF-like kinases as upstream factors that activate the SnRK2 kinases. We also elucidated the activation mechanisms of the transcription factor DREB2A, which plays important roles in drought and heat stress-inducible gene expression. Furthermore, it was proved that two independent signal transduction pathways exist in the mechanism of cold-inducible gene expression in plants. On the other hand, we also succeeded in identifying the regulatory factors that regulate the activation of the high temperature stress-activated retrotransposon ONSEN.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), The University of Tokyo, 15H05960
  • 環境ストレス活性型トランスポゾンを用いたストレス耐性植物の作出
    「形質転換植物デザイン研究拠点」平成30年度 共同利用・共同研究
    Apr. 2019 - Mar. 2020
    伊藤 秀臣; 三浦 謙治; 野沢 紘佑
    筑波大学遺伝子実験センター, 筑波大学遺伝子実験センター, Principal investigator, Competitive research funding
  • 環境ストレス活性型転移因子を制御するクロマチン構造の解析
    令和元年度 岡山大学資源植物科学研究所 共同研究
    Apr. 2019 - Mar. 2020
    伊藤 秀臣、池田 陽子
    岡山大学資源植物科学研究所, 岡山大学資源植物科学研究所, Principal investigator, 3109
  • 高温ストレス活性型レトロトランスポゾンのエピジェネティックな転移制御機構の解析
    2019年度国立遺伝学研究所共同研究（A）
    Apr. 2019 - Mar. 2020
    伊藤 秀臣; 角谷 徹仁; 稲垣 宗一; 野沢 紘佑
    国立遺伝学研究所, 国立遺伝学研究所, Principal investigator, Competitive research funding
  • 高温活性型トランスポゾンを用いた乾燥耐性植物の作出
    鳥取大学乾燥地研究センター共同利用研究
    Apr. 2018 - Mar. 2020
    伊藤 秀臣、辻本 壽
    鳥取大学乾燥地研究センター, 鳥取大学乾燥地研究センター, Principal investigator
  • Young teacher overseas dispatch support project encouraged by the President
    Young teacher overseas dispatch support project encouraged by the President
    Apr. 2018 - Mar. 2020
    Hidetaka Ito
    Hokkaido University, Principal investigator, Competitive research funding
  • 環境ストレス活性型転移因子を制御するクロマチン構造の解析
    平成30年度 岡山大学資源植物科学研究所 共同研究
    Apr. 2018 - Mar. 2019
    伊藤 秀臣、池田 陽子
    岡山大学資源植物科学研究所, 岡山大学資源植物科学研究所, Principal investigator, 3010
  • 高温ストレス活性型レトロトランスポゾンのエピジェネティックな転移制御機構の解析
    2018年度国立遺伝学研究所共同研究（Ｂ）
    Apr. 2018 - Mar. 2019
    伊藤 秀臣; 角谷 徹仁; 稲垣 宗一; 野沢 紘佑
    国立遺伝学研究所, 国立遺伝学研究所, Principal investigator, Competitive research funding
  • 環境ストレス活性型トランスポゾンを用いたストレス耐性植物の作出
    「形質転換植物デザイン研究拠点」平成29年度 共同利用・共同研究
    Apr. 2017 - Mar. 2018
    伊藤 秀臣; 三浦 謙治; 野沢 紘佑
    筑波大学遺伝子実験センター, 筑波大学遺伝子実験センター, Principal investigator, Competitive research funding
  • 環境ストレス活性型転移因子を制御するクロマチン構造の解析
    平成29年度 岡山大学資源植物科学研究所 共同研究
    Apr. 2017 - Mar. 2018
    伊藤 秀臣,池田 陽子
    岡山大学資源植物科学研究所, 岡山大学資源植物科学研究所, Principal investigator, 2908
  • 高温ストレス活性型レトロトランスポゾンのエピジェネティックな転移制御機構の解析
    2017年度国立遺伝学研究所共同研究（Ａ）
    Apr. 2017 - Mar. 2018
    伊藤 秀臣; 角谷 徹仁; 稲垣 宗一
    国立遺伝学研究所, 国立遺伝学研究所, Principal investigator, Competitive research funding
  • Genomic breeding using high temperature activated transposable elements
    Research Grants
    Apr. 2017 - Mar. 2018
    Hidetaka Ito
    The Suhara Memorial Foundation, Principal investigator, Competitive research funding
  • 高温活性型トランスポゾンを用いた乾燥耐性植物の作出
    鳥取大学乾燥地研究センター共同利用研究
    Apr. 2016 - Mar. 2018
    伊藤 秀臣; 岡本 昌憲; 辻本 壽
    鳥取大学乾燥地研究センター, 鳥取大学乾燥地研究センター, Principal investigator
  • 環境ストレス活性型トランスポゾンを用いたストレス耐性植物の作出
    「形質転換植物デザイン研究拠点」平成28年度 共同利用・共同研究
    Apr. 2016 - Mar. 2017
    伊藤 秀臣; 三浦 謙治; 野沢 紘佑
    筑波大学遺伝子実験センター, 筑波大学遺伝子実験センター, Principal investigator, Competitive research funding
  • 熱活性型トランスポゾンの転移とその制御機構の解明
    2016年度国立遺伝学研究所共同研究（A1）
    Apr. 2016 - Mar. 2017
    伊藤 秀臣; 角谷 徹仁; 稲垣 宗一; 伊藤 佑
    国立遺伝学研究所, 国立遺伝学研究所, Principal investigator, Competitive research funding
  • 環境ストレス活性型トランスポゾンを用いたストレス耐性植物の作出
    「形質転換植物デザイン研究拠点」平成27年度 共同利用・共同研究
    Apr. 2015 - Mar. 2016
    伊藤 秀臣; 三浦 謙治; 野沢 紘佑
    筑波大学遺伝子実験センター, 筑波大学遺伝子実験センター, Principal investigator, Competitive research funding
  • メリステム特異的なストレス活性型トランスポゾン制御機構の解明
    平成27年度 岡山大学資源植物科学研究所 共同研究
    Apr. 2015 - Mar. 2016
    伊藤 秀臣、馬 建鋒
    岡山大学資源植物科学研究所, 岡山大学資源植物科学研究所, Principal investigator, 2713
  • 熱活性型トランスポゾンの転移とその制御機構の解明
    2015年度国立遺伝学研究所共同研究（A1）
    Apr. 2015 - Mar. 2016
    伊藤 秀臣; 角谷 徹仁; 樽谷 芳明
    国立遺伝学研究所, 国立遺伝学研究所, Principal investigator, Competitive research funding
  • Application of environmental stress-activated transposable elements to breeding
    talent grants
    Apr. 2015 - Mar. 2016
    Hidetaka Ito
    North Tech Foundation, Principal investigator, Competitive research funding
  • Genomic changes and environmental adaptation in plants induced by environmental stress-activated transposable elements
    Scientific Research Grants New Academic Field Research (Research Field Proposal Type)
    01 Apr. 2013 - 31 Mar. 2015
    Hidetaka Ito
    １、トランスポゾンの転写を制御する機構の解明
    高温ストレスで活性化するトランスポゾン(ONSEN)において、その転写活性を制御する因子の同定を行うためにONSENのプロモーター領域にGFP遺伝子マーカーを融合させた形質転換体を作成し、変異原(EMS)処理を行い、ONSENが常温で活性化するような変異体の探索を行った。現在までに独立した系で複数の候補個体を得ている。候補個体を自殖させた個体でGFP遺伝子の活性化の再現性を確認できた。今後、原因遺伝子を同定し、ONSENの転写制御を担っている因子の明らかにする。
    ２、トランスポゾンの転移を制御する機構の解明
    種子及び植物の細胞を脱分化させたカルスを誘導し、未分化細胞でのONSENの転移を解析した。その結果、カルスから再分化させた植物体でONSENの転移が確認された。興味深いことにカルス誘導を介したONSENの転移はRNA干渉を担う遺伝子の変異体のみならず、野生型でも起こることがわかった。このことから，分化細胞と脱分化細胞ではトランスポゾンの制御機構に違いがあることが示唆された。次世代シーケンサーを用いて、転移先を同定した結果、多くが遺伝子内部に挿入していることが分かった。カルス由来の挿入領域の遺伝子発現を解析するため野生型転移集団を用いてマイクロアレイ解析により遺伝子発現解析を行った。その結果、ONSENの挿入による遺伝子発現の変化は少数でそのほとんどが発現の低下であったが、一つの遺伝子で発現の上昇が見られた。
    Japan Society for the Promotion of Science, 新学術領域研究(研究領域提案型), Hokkaido University, 25119701
  • 環境ストレス活性型トランスポゾンを用いたストレス耐性植物の作出
    平成26年度「形質転換植物デザイン研究拠点」共同利用・共同研究
    Apr. 2014 - Mar. 2015
    伊藤 秀臣; 三浦 謙治; 佐藤 澪
    筑波大学遺伝子実験センター, 筑波大学遺伝子実験センター, Principal investigator
  • メリステム特異的なストレス活性型トランスポゾン制御機構の解明
    平成26年度 岡山大学資源植物科学研究所 共同研究
    Apr. 2014 - Mar. 2015
    伊藤 秀臣,馬 建鋒
    岡山大学資源植物科学研究所, 岡山大学資源植物科学研究所, Principal investigator, 2612
  • 熱活性型トランスポゾンの転移とその制御機構の解明
    2014年度国立遺伝学研究所共同研究（A）
    Apr. 2014 - Mar. 2015
    伊藤 秀臣; 角谷 徹仁; 樽谷 芳明
    国立遺伝学研究所, 国立遺伝学研究所, Principal investigator, Competitive research funding
  • Transposons and host genomic responses to environmental change
    Precursory Research for Embryonic Science and Technology
    Oct. 2011 - Mar. 2015
    Hidetaka Ito
    Japan Science and Technology Agency, Principal investigator, Competitive research funding
  • メリステム特異的なストレス活性型トランスポゾン制御機構の解明
    平成25年度 岡山大学資源植物科学研究所 共同研究
    Apr. 2013 - Mar. 2014
    伊藤 秀臣; 馬 建鋒
    岡山大学資源植物科学研究所, 岡山大学資源植物科学研究所, Principal investigator, 2510
  • 環境ストレスにより活性化するトランスポゾンとゲノムの安定性
    2013年度国立遺伝学研究所共同研究（B）
    Apr. 2013 - Mar. 2014
    伊藤 秀臣; 角谷 徹仁; 樽谷 芳明
    国立遺伝学研究所, 国立遺伝学研究所, Principal investigator, Competitive research funding
  • Genomic changes and stress responses of plants in response to environmental changes
    Grant-in-Aid for Scientific Research on Innovative Areas (Research Area Proposal Type)
    01 Apr. 2011 - 31 Mar. 2013
    Hidetaka Ito
    地球の温暖化など地球環境の破壊が問題となっている昨今、環境ストレスと生物の関係はどうなっているのか？そのような課題から環境の変化がもたらす影響について研究を行った。その結果、環境の変化が植物の進化に積極的な影響力を持つことをはじめて実証した。環境ストレスである高温ストレスを植物に与えると転移因子（トランスポゾン）が活性化し、その子孫でトランスポゾンの挿入によるゲノム構造の変化が起ることを見つけた。このことはゲノム構造の変化によって引き起こされる様々な変異の中から環境ストレスに対する耐性を獲得した植物が誕生する可能性を示唆している。本研究は環境ストレスが植物に与える影響について「ゲノム構造の変化と環境適応」という側面からアプローチし、ストレス条件下で活性化するトランスポゾンとそれを制御する宿主側の遺伝子の解析を行うことにより大地・大気環境の変動に対処するために植物がもつ巧妙な生存戦略について理解し、最終的には実際にストレスにより活性化するトランスポゾンを用い植物のゲノム構造を変化させ環境適応能力を獲得した個体を作り上げることを目的とした。研究成果として、熱ストレスによりトランスポゾンの転移が引き起こされた集団からABAストレス耐性個体を同定し、その原因となる挿入先を次世代シークエンサーを用いたゲノム解析により明らかにした。マイクロアレイの解析結果から、ABAストレスで活性化するいくつかのトランスポゾンを同定した。トランスポゾン挿入領域のエピジェネティックな修飾の変化を解析するため、ヒストンH3K9やH3K4の抗体を用いた免疫沈降実験を行った。
    Japan Society for the Promotion of Science, 新学術領域研究(研究領域提案型), Hokkaido University, Principal investigator, Competitive research funding, 23119501
  • 環境ストレスにより活性化するトランスポゾンとゲノムの安定性
    2012年度国立遺伝学研究所共同研究（B）
    Apr. 2012 - Mar. 2013
    伊藤 秀臣; 角谷 徹仁; 河邉 昭
    国立遺伝学研究所, 国立遺伝学研究所, Principal investigator
  • 環境ストレスにより活性化するトランスポゾンとゲノムの安定性
    2011年度国立遺伝学研究所共同研究（B）
    Apr. 2011 - Mar. 2012
    伊藤 秀臣; 角谷 徹仁; 河邊 昭
    国立遺伝学研究所, 国立遺伝学研究所, Principal investigator, Competitive research funding
  • Studies on the regulatory mechanism of transposons activated by environmental stress
    Research Grants (Scholarship)
    Apr. 2011 - Mar. 2012
    Hidetaka Ito
    The Akiyama Life Science Foundation, Principal investigator, Competitive research funding
  • Evolution of plant genome promoted by an environmental stress
    Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
    2011 - 2012
    ITO Hidetaka
    The effect of environmental stress to plant genome was demonstrated using a heat-activated transposon. The thermal sensitivity of the transposon activity was analyzed and found a threshold. The heat-activated transposon was conserved among Brassica species and a heat-activation was detected on some species.
    Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, Principal investigator, Competitive research funding, 23770034
  • An siRNA pathway controls retrotransposition in plants subjected to stress
    平成23年度加藤記念国際交流助成金
    Jun. 2011
    加藤記念バイオサイエンス研究振興財団, Principal investigator, Competitive research funding, ［Internationally co-authored］
  • 環境ストレスにより活性化するトランスポゾンとゲ ノム構造の解析
    2010年度国立遺伝学研究所共同研究（Ａ）
    Apr. 2010 - Mar. 2011
    伊藤 秀臣; 角谷 徹仁
    国立遺伝学研究所, 国立遺伝学研究所, Principal investigator
■ Industrial Property Rights
  • 植物においてレトロトランスポゾンを活性化させるための剤またはその方法
    Patent right, 伊藤 秀臣; バルバルア バイバフ ラジ
    特願2025-139232
    Domestic, Domestic
  • 植物のストレス耐性を誘導するための剤又はその方法
    Patent right, 伊藤 秀臣; バルバルア バイバフ ラジ
    特願2025-139234
    Domestic, Domestic