博士（工学）, 九州大学

タンパク質機能化

バイオ界面

バイオマス

レアメタル

酵素工学

バイオミネラリゼーション

Environmental Science/Agriculture Science, Environmental materials and recycle technology

Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering), Biofunction and bioprocess engineering

Bachelor's degree program, School of Engineering

Master's degree program, Graduate School of Engineering

Doctoral (PhD) degree program, Graduate School of Engineering

2022, 第45回生物工学奨励賞（照井賞）
有機–無機界面に着目した複合バイオ材料の創製に関する生物化学工学的研究

2012, 平成23年度化学工学会研究奨励賞（實吉雅郎記念賞）
Japan

2009, Best Presentation Award, APBioChEC'09
Japan

2006, Best Ph.D student oral presentation, YABEC 2006 Symposium

2005, 第12回日本生物工学会九州支部佐賀大会 学生賞（博士の部）
Japan

Synergistic poly-tannic acid/lignin-coated cellulose composites for the simultaneous removal of heavy metals and phenol
Ilham Maulidin; Chikara Takano; Kazunori Nakashima
Journal of Environmental Chemical Engineering, 14, 2, 121540, 121540, Elsevier BV, Apr. 2026, [Peer-reviewed]
Scientific journal

Development of acid mine drainage treatment process using arsenic adsorbing bacteria
Sohei Iwama; Chikara Takano; Satoru Kawasaki; Kazunori Nakashima
Journal of Environmental Chemical Engineering, 13, 6, 119222, 119222, Elsevier BV, Dec. 2025, [Peer-reviewed]
Scientific journal

Metal tolerance and recovery mechanisms of Priestia sp. Mn7 under pH 1.5
Chikara Takano; Kazunori Nakashima; Hideki Aoyagi; Takaaki Kojima; Satoru Kawasaki
Extremophiles, 29, 3, Springer Science and Business Media LLC, 28 Oct. 2025, [Peer-reviewed]
Scientific journal

Protein- and Polysaccharide-Based Heavy Metal Removal Materials: Batch and Continuous Systems
Chikara Takano; Yuki Shigemasa; Kousuke Aoki; Ilham Maulidin; Satoru Kawasaki; Kazunori Nakashima
ACS Sustainable Resource Management, American Chemical Society (ACS), 25 Oct. 2025, [Peer-reviewed]
Scientific journal

In situ mineral formation on a plate for direct and efficient screening of bacteria suitable for biocementation
Kazunori Nakashima; Yuta Sugawara; Masahiro Fujita; Aya Yaegashi; Meiqi Chen; Sivakumar Gowthaman; Thiloththama H. K. Nawarathna; Wilson Mwandira; Numbi Ramudu Kamini; Parthasarathy Baskaran Sujiritha; Chikara Takano; Satoru Kawasaki
Scientific Reports, 15, 1, 18706, Springer Science and Business Media LLC, 28 May 2025, [Peer-reviewed]
Scientific journal

Mussel Adhesive Protein-Assisted Magnetic Recovery of Microplastics from Aquatic Environments
Anju Pilakka Veedu; Kazunori Nakashima; Takahiro Sato; Ami Sasabe; Keita Suzuki; Chikara Takano; Satoru Kawasaki
ACS ES&T Water, American Chemical Society (ACS), 07 Apr. 2025, [Peer-reviewed]
Scientific journal

Exploration of ureolytic airborne bacteria for biocementation applications from different climate zones in Japan
Meiqi Chen; Chikara Takano; Kazunori Nakashima; Sivakumar Gowthaman; Satoru Kawasaki
Scientific Reports, 15, 1, Springer Science and Business Media LLC, 04 Mar. 2025, [Peer-reviewed]
Scientific journal

Arsenic Removal From Acid Mine Drainage Using Acid‐Tolerant Bacteria
Sohei Iwama; Chikara Takano; Kazunori Nakashima; Hideki Aoyagi; Satoru Kawasaki
CLEAN – Soil, Air, Water, 53, 1, Wiley, 27 Jan. 2025, [Peer-reviewed]
Scientific journal, ABSTRACT

Acid mine drainage (AMD) has a low pH and contains harmful metals, making it a severe problem in the mining industry. Neutralization treatment using slaked lime is widely applied to remove potentially toxic metals and adjust the pH. However, this generates neutralized sludge containing large amounts of harmful metals. Therefore, in this study, we propose a bacterial bioprocess for removing metals before neutralization. Acid‐ and metal‐tolerant bacteria were isolated from neutral soils and utilized as As removers from AMD. The Paenathrobacter sp. strain H1 removed As (43.6%) and Fe (10.6%) from AMD in a single‐batch test (pH 1.95; initial concentrations were 6.13 and 283 mg L⁻¹, respectively). Repeated batch tests using fresh cells enhanced the As removal ratio, achieving successful removal of As (95.3%) and Fe (75.5%). Although further research is required, this study has substantial implications for the development of a sustainable AMD treatment to suppress harmful waste generation.

Proposing a new sustainable approach for sand improvement using biologically-derived calcium phosphate cement
Sivakumar Gowthaman; Yuta Kumamoto; Kazunori Nakashima; Chikara Takano; Satoru Kawasaki
Biogeotechnics, 100135, 100135, Elsevier BV, Jan. 2025, [Peer-reviewed]
Scientific journal

Effect of humic acid on soil solidification by enzyme induced carbonate precipitation
Meiqi Chen; Aoi Ichinohe; Kazunori Nakashima; Chikara Takano; Sivakumar Gowthaman; Lutfian R. Daryono; Satoru Kawasaki
Biogeotechnics, 100133, 100133, Elsevier BV, Jan. 2025, [Peer-reviewed]
Scientific journal

Feasibility study of enhancing enzyme-induced carbonate precipitation with eggshell waste for sand solidification
Zhen Yan; Kazunori Nakashima; Chikara Takano; Satoru Kawasaki
Biogeotechnics, 2, 4, 100108, 100108, Elsevier BV, Dec. 2024, [Peer-reviewed]
Scientific journal

Utilisation of acid-tolerant bacteria for base metal recovery under strongly acidic conditions
Chikara Takano; Kazunori Nakashima; Satoru Kawasaki; Hideki Aoyagi
Extremophiles, 28, 3, Springer Science and Business Media LLC, 24 Sep. 2024, [Peer-reviewed]
Scientific journal

Ecofriendly solidification of sand using microbially induced calcium phosphate precipitation
Maksym Avramenko; Kazunori Nakashima; Chikara Takano; Satoru Kawasaki
Scientific Reports, 14, 1, Springer Science and Business Media LLC, 30 May 2024, [Peer-reviewed]
Scientific journal, Abstract

This study introduces microbiologically induced calcium phosphate precipitation (MICPP) as a novel and environmentally sustainable method of soil stabilization. Using Limosilactobacillus sp., especially NBRC 14511 and fish bone solution (FBS) extracted from Tuna fish bones, the study was aimed at testing the feasibility of calcium phosphate compounds (CPCs) deposition and sand stabilization. Dynamic changes in pH and calcium ion (Ca²⁺) concentration during the precipitation experiments affected the precipitation and sequential conversion of dicalcium phosphate dihydrate (DCPD) to hydroxyapatite (HAp), which was confirmed by XRD and SEM analysis. Sand solidification experiments demonstrated improvements in unconfined compressive strength (UCS), especially at higher Urea/Ca²⁺ ratios. The UCS values obtained were 10.35 MPa at a ratio of 2.0, 3.34 MPa at a ratio of 1.0, and 0.43 MPa at a ratio of 0.5, highlighting the advantages of MICPP over traditional methods. Microstructural analysis further clarified the mineral composition, demonstrating the potential of MICPP in environmentally friendly soil engineering. The study highlights the promise of MICPP for sustainable soil stabilization, offering improved mechanical properties and reducing environmental impact, paving the way for novel geotechnical practices.

Biosynthesis of a Functional Silica-Based Biohybrid Material with High Pb(II) Capture Ability
Ilham Maulidin; Kazunori Nakashima; Ryo Naota; Anju Pilakka Veedu; Chikara Takano; Satoru Kawasaki
ACS Sustainable Resource Management, American Chemical Society (ACS), 07 May 2024, [Peer-reviewed]
Scientific journal

Combination of cellulose nanofiber and artificial fusion protein for biocementation
Thiloththama Hiranya Kumari Nawarathna; Jin Sakai; Kazunori Nakashima; Tetsuya Kawabe; Miki Shikama; Chikara Takano; Satoru Kawasaki
Frontiers in Built Environment, 9, Frontiers Media SA, 08 Jan. 2024, [Peer-reviewed]
Scientific journal, Biomineralization occurring in living organisms is mostly controlled by organic macromolecules such as polysaccharides and proteins. Recently, biomineralization has been attracting much attention as a green and sustainable cementation technique including enzyme-induced carbonate precipitation (EICP), where CaCO₃ is formed by hydrolysis of urea by urease in the presence of calcium ions. In this study, we have developed a novel hybrid biocementation method combining CaCO₃ and cellulose nanofiber (CNF). In nature, matrix proteins work as a binder at the interface of organic and inorganic materials to form hybrid biomaterials. By mimicking the natural system, we designed an artificial fusion protein to facilitate the deposition of CaCO₃ on CNF. Calcite-binding peptide (CaBP) and carbohydrate-binding module (CBM) were introduced in the artificial fusion protein CaBP-CBM to connect CaCO₃ and cellulose. The addition of CNF in the EICP system resulted in the formation of a number of small particles of CaCO₃ compared to a non-additive system. The addition of the fusion protein CaBP-CBM to CNF led to an increase in the size of CaCO₃ particles. Furthermore, the combination of CaBP-CBM and CNF provides higher strength of samples in sand solidification. Therefore, introduction of CNF and the fusion protein would be promising for novel biocementation techniques.

Baseline investigation on enzyme induced calcium phosphate precipitation for solidification of sand
Sivakumar Gowthaman; Moeka Yamamoto; Meiqi Chen; Kazunori Nakashima; Satoru Kawasaki
Frontiers in Built Environment, 9:1307650, Dec. 2023, [Peer-reviewed]
English, Scientific journal

Eco-friendly soil stabilization method using fish bone as cement material
Maksym Avramenko; Kazunori Nakashima; Chikara Takano; Satoru Kawasaki
Science of The Total Environment, 900, 165823, 165823, Elsevier BV, Nov. 2023, [Peer-reviewed]
Scientific journal

Functional modification of mussel adhesive protein to control solubility and adhesion property
Anju Pilakka Veedu; Kazunori Nakashima; Hayahide Shiga; Takahiro Sato; Kasun Godigamuwa; Naoki Hiroyoshi; Satoru Kawasaki
Journal of Bioscience and Bioengineering, 136, 2, 87, 93, Elsevier BV, Aug. 2023, [Peer-reviewed]
Scientific journal

Baseline investigation on soil solidification through biocementation using airborne bacteria
Meiqi Chen; Sivakumar Gowthaman; Kazunori Nakashima; Chikara Takano; Satoru Kawasaki
Frontiers in Bioengineering and Biotechnology, 11, Frontiers Media SA, 14 Jun. 2023, [Peer-reviewed]
Scientific journal, Microbial induced carbonate precipitation (MICP) through the ureolysis metabolic pathway is one of the most studied topics in biocementation due to its high efficiency. Although excellent outcomes have proved the potential of this technique, microorganisms face some obstacles when considering complicated situations in the real field, such as bacterial adaptability and survivability issues. This study made the first attempt to seek solutions to this issue from the air, exploring ureolytic airborne bacteria with resilient features to find a solution to survivability issues. Samples were collected using an air sampler in Sapporo, Hokkaido, a cold region where sampling sites were mostly covered with dense vegetation. After two rounds of screening, 12 out of 57 urease-positive isolates were identified through 16S rRNA gene analysis. Four potentially selected strains were then evaluated in terms of growth pattern and activity changes within a range of temperatures (15°C–35°C). The results from sand solidification tests using two Lederbergia strains with the best performance among the isolates showed an improvement in unconfined compressive strength up to 4–8 MPa after treatment, indicating a high MICP efficiency. Overall, this baseline study demonstrated that the air could be an ideal isolation source for ureolytic bacteria and laid a new pathway for MICP applications. More investigations on the performance of airborne bacteria under changeable environments may be required to further examine their survivability and adaptability.

Field experimentation of bio-cementation using low-cost cementation media for preservation of slope surface
Sivakumar Gowthaman; Hiromu Koizumi; Kazunori Nakashima; Satoru Kawasaki
Case Studies in Construction Materials, 18, e02086, Apr. 2023, [Peer-reviewed]
English, Scientific journal

SOIL IMPROVEMENT USING CALCIUM PHOSPHATE COMPOUNDS AS A NOVEL SUSTAINABLE METHOD: A REVIEW
Maksym Avramenko
International Journal of GEOMATE, 24, 101, International Journal of Geomate, 30 Jan. 2023, [Peer-reviewed]
Scientific journal

Naturally Derived Cements Learned from the Wisdom of Ancestors: A Literature Review Based on the Experiences of Ancient China, India and Rome
Zhan Su; Zhen Yan; Kazunori Nakashima; Chikara Takano; Satoru Kawasaki
Materials, 16, 2, 603, 603, MDPI AG, 08 Jan. 2023, [Peer-reviewed]
Scientific journal, For over a thousand years, many ancient cements have remained durable despite long-term exposure to atmospheric or humid agents. This review paper summarizes technologies of worldwide ancient architectures which have shown remarkable durability that has preserved them over thousands of years of constant erosion. We aim to identify the influence of organic and inorganic additions in altering cement properties and take these lost and forgotten technologies to the production frontline. The types of additions were usually decided based on the local environment and purpose of the structure. The ancient Romans built magnificent structures by making hydraulic cement using volcanic ash. The ancient Chinese introduced sticky rice and other local materials to improve the properties of pure lime cement. A variety of organic and inorganic additions used in traditional lime cement not only changes its properties but also improves its durability for centuries. The benefits they bring to cement may also be useful in enzyme-induced carbonate precipitation (EICP) and microbially induced carbonate precipitation (MICP) fields. For instance, sticky rice has been confirmed to play a crucial role in regulating calcite crystal growth and providing interior hydrophobic conditions, which contribute to improving the strength and durability of EICP- and MICP-treated samples in a sustainable way.

Interfacial biosilica coating of chitosan gel using fusion silicatein to fabricate robust hybrid material for biomolecular applications
Kasun Godigamuwa; Kazunori Nakashima; Sota Tsujitani; Ryo Naota; Ilham Maulidin; Satoru Kawasaki
Journal of Materials Chemistry B, 11, 8, 1654, 1658, Royal Society of Chemistry (RSC), 2023, [Peer-reviewed]
Scientific journal, An enzyme-encapsulated silica-based hybrid material was developed using a chitosan gel.

Valorization of tannery solid wastes for sustainable enzyme induced carbonate precipitation process
Parthasarathy Baskaran Sujiritha; Vijan Lal Vikash; George Sebastian Antony; Ganesan Ponesakki; Niraikulam Ayyadurai; Kazunori Nakashima; Numbi Ramudu Kamini
Chemosphere, 308, 136533, 136533, Elsevier BV, Dec. 2022, [Peer-reviewed], ［Internationally co-authored］
Scientific journal

Polymer-assisted enzyme induced carbonate precipitation for non-ammonia emission soil stabilization
Zhen Yan; Sivakumar Gowthaman; Kazunori Nakashima; Satoru Kawasaki
Scientific Reports, 12, 1, Springer Science and Business Media LLC, Dec. 2022, [Peer-reviewed]
Scientific journal, Abstract

Biocementation using enzyme induced carbonate precipitation (EICP) process has become an innovative method for soil improvement. One of the major limitations in scaling-up of biocement treatment is the emission of gaseous ammonia during the urea hydrolysis, which is environmentally hazardous. In order to eliminate this shortcoming, this paper presents a series of experiments performed to evaluate a novel approach for preventing the ammonia byproducts in the EICP process via the use of polyacrylic acid (PAA). Through the adjustment of the pH to acidic, PAA not only promotes the enzyme activity, but also averts the conversion of ammonium to gaseous ammonia and its release, thus preventing any harm to the environment. The sand samples were treated with cementation solution and assessed for improvement in strength. Calcium carbonate content measurements and X-ray powder diffraction analysis identified the calcite crystals precipitated in the soil pores. Scanning electron microscopy analysis clearly showed that calcium carbonate was precipitated connecting soil particles, thus providing a uniaxial compressive strength (UCS) of up to 1.65 MPa. Overall, the inhibition in the speciation of gaseous ammonia shows the great potential of PAA for large-scale promotion of biocement.

Feasibility of bacterial-enzyme induced carbonate precipitation technology for stabilizing fine-grained slope soils
Sivakumar Gowthaman; Takashi Iki; Aoi Ichinohe; Kazunori Nakashima; Satoru Kawasaki
Frontiers in Built Environment, 8, Frontiers Media SA, 12 Oct. 2022, [Peer-reviewed]
Scientific journal, Microbial Induced Carbonate Precipitation (MICP) has recently become a new technology for stabilizing the slope surface. The applicability of MICP, however, is limited in fine-grained soils due to the restrictions regarding the transportation of bacteria cells. The purpose of this study was to assess the feasibility of an alternative called Bacterial-Enzyme Induced Carbonate Precipitation (B-EICP) for stabilizing the fine-grained slope soils. Unlike the MICP strategy (involving whole-cells of bacteria), the proposed B-EICP utilizes bacterial urease to induce the bio-cement formation within soil. The whole-cell culture of Lysinibacillus xylanilyticus was subjected to cyclic sonication to extract the free urease suspension. The B-EICP treatment was performed to the columns prepared using two different soils obtained from representative expressway slopes. The cementation responses of the proposed B-EICP were compared with that of typical MICP method, especially from the following viewpoints, (i) adaptability to soil with high fine-grained content, (ii) conditions under which B-EICP can be effectively applied and (iii) cementation under low temperature. The results revealed that the extract solution had higher urease activity compared to original bacteria culture, and the activity remained more stable at low temperature conditions (15°C). The results further confirmed that B-EICP method is more suitable for stabilizing soils with large amount of fine particles. Comparing with MICP, the B-EICP resulted higher unconfined compressive strength (over 1200 kPa) and deeper cementation in the silty sand. Microscale analysis suggested that the B-EICP could induce smaller calcium carbonate crystals than that by MICP, but the number of crystals in B-EICP were significantly more, thus contributed to increased particle-particle cementation.

State-of-the-Art Review on Engineering Uses of Calcium Phosphate Compounds: An Eco-Friendly Approach for Soil Improvement
Maksym Avramenko; Kazunori Nakashima; Satoru Kawasaki
Materials, 15, 19, 6878, 6878, MDPI AG, 03 Oct. 2022, [Peer-reviewed]
Scientific journal, Greenhouse gas emissions are a critical problem nowadays. The cement manufacturing sector alone accounts for 8% of all human-generated emissions, and as the world’s population grows and globalization intensifies, this sector will require significantly more resources. In order to fulfill the need of geomaterials for construction and to reduce carbon dioxide emissions into the atmosphere, conventional approaches to soil reinforcement need to be reconsidered. Calcium phosphate compounds (CPCs) are new materials that have only recently found their place in the soil reinforcement field. Its eco-friendly, non-toxic, reaction pathway is highly dependent on the pH of the medium and the concentration of components inside the solution. CPCs has advantages over the two most common environmental methods of soil reinforcement, microbial-induced carbonate precipitation (MICP) and enzyme induced carbonate precipitation (EICP); with CPCs, the ammonium problem can be neutralized and thus allowed to be applied in the field. In this review paper, the advantages and disadvantages of the engineering uses of CPCs for soil improvement have been discussed. Additionally, the process of how CPCs perform has been studied and an analysis of existing studies related to soil reinforcement by CPC implementation was conducted.

Influence of humic acid on microbial induced carbonate precipitation for organic soil improvement
Meiqi Chen; Sivakumar Gowthaman; Kazunori Nakashima; Satoru Kawasaki
Environmental Science and Pollution Research, 30, 6, 15230, 15240, Springer Science and Business Media LLC, 27 Sep. 2022, [Peer-reviewed]
Scientific journal

Effect of wetting and drying cycles on the durability of bio-cemented soil of expressway slope
S. Gowthaman; K. Nakashima; S. Kawasaki
International Journal of Environmental Science and Technology, 19, 4, 2309, 2322, Springer Science and Business Media LLC, Apr. 2022, [Peer-reviewed]
Scientific journal, Abstract

Cyclic wet-dry is one of the influential weathering agents which can rapidly alter the mechanical properties of soils, limiting their durability and consistent performance. This study investigates the effect of wet-dry cycles on the mechanical behaviour of bio-cemented soil. Microbial-induced carbonate precipitation-based bio-cementation is an innovative soil improvement method, which is gaining increasing attention as a potential alternative for stabilizing slope surface. As the treated surfaces are exposed to repeated rainfalls and draughts, durability analysis is essential; cyclic wet-dry tests were therefore performed as a credible indicator of durability. The soil obtained from the Hokkaido expressway slope was treated at laboratory to varying cementation levels (% CaCO₃) and subjected to 50 subsequent wet-dry cycles. Physical and mechanical changes were monitored using mass loss, shear wave velocities and needle penetration tests during wet-dry cycles. The results showed that the wet-dry cycles deteriorated the physical and mechanical at two stages. The mass and S-wave velocity of specimens significantly dropped after first few cycles and then tended to reach equilibrium. The second stage of notable deterioration was observed between 30 and 50 wet-dry cycles. It is suggested that the erosion of weak and powdery deposition of CaCO₃ causes the degradation at the early stage, whereas the degradation in the late stage was attributed to the microstructural deformations of intact carbonate bonds. It was also found that the increase in cementation level decreases the deterioration of bio-cemented soil under wet-dry cycles.

Durability Improvement of Biocemented Sand by Fiber-Reinforced MICP for Coastal Erosion Protection
Md Al Imran; Kazunori Nakashima; Niki Evelpidou; Satoru Kawasaki
Materials, 15, 7, 2389, 2389, MDPI AG, 24 Mar. 2022, [Peer-reviewed]
Scientific journal, Soil improvement via MICP (microbially induced carbonate precipitation) technologies has recently received widespread attention in the geoenvironmental and geotechnical fields. The durability of MICP-treated samples remains a critical concern in this novel method. In this work, fiber (jute)-reinforced MICP-treated samples were investigated to evaluate their durability under exposure to distilled water (DW) and artificial seawater (ASW), so as to advance the understanding of long-term performance mimicking real field conditions, along with improvement of the MICP-treated samples for use in coastal erosion protection. Primarily, the results showed that the addition of fiber (jute) improved the durability of the MICP-treated samples by more than 50%. Results also showed that the wet–dry (WD) cyclic process resulted in adverse effects on the mechanical and physical characteristics of fiber-reinforced MICP-treated samples in both DW and ASW. The breakdown of calcium carbonates and bonding effects in between the sand particles was discovered to be involved in the deterioration of MICP samples caused by WD cycles, and this occurs in two stages. The findings of this study would be extremely beneficial to extend the insight and understanding of improvement and durability responses for significant and effective MICP treatments and/or re-treatments.

Solubilization and aggregation control of silica-polymerizing enzyme fused with a removable soluble protein
Hidetoshi Oguri; Kazunori Nakashima; Kasun Godigamuwa; Junnosuke Okamoto; Yudai Takeda; Fumiyoshi Okazaki; Masafumi Sakono; Satoru Kawasaki
Journal of Bioscience and Bioengineering, 133, 3, 222, 228, Elsevier BV, Mar. 2022, [Peer-reviewed]
Scientific journal

Calcium phosphate biocement using bone meal and acid urease: An eco-friendly approach for soil improvement
Sivakumar Gowthaman; Moeka Yamamoto; Kazunori Nakashima; Volodymyr Ivanov; Satoru Kawasaki
Journal of Cleaner Production, 319, 128782, 128782, Elsevier BV, Oct. 2021, [Peer-reviewed]
Scientific journal

Removal of ammonium by-products from the effluent of bio-cementation system through struvite precipitation
Sivakumar Gowthaman; Arash Mohsenzadeh; Kazunori Nakashima; Satoru Kawasaki
Materials Today: Proceedings, Elsevier BV, Sep. 2021, [Peer-reviewed]
Scientific journal

Experimental Study on Sand Stabilization Using Bio-Cementation with Wastepaper Fiber Integration
Meiqi Chen; Sivakumar Gowthaman; Kazunori Nakashima; Shin Komatsu; Satoru Kawasaki
MATERIALS, 14, 18, Sep. 2021, [Peer-reviewed]
English, Scientific journal

Fabrication of silica on chitin in ambient conditions using silicatein fused with a chitin-binding domain
Kasun Godigamuwa; Kazunori Nakashima; Sota Tsujitani; Satoru Kawasaki
BIOPROCESS AND BIOSYSTEMS ENGINEERING, 44, 9, 1883, 1890, Sep. 2021, [Peer-reviewed]
English, Scientific journal

Durability analysis of bio-cemented slope soil under the exposure of acid rain
Sivakumar Gowthaman; Kazunori Nakashima; Satoru Kawasaki
JOURNAL OF SOILS AND SEDIMENTS, 21, 8, 2831, 2844, Aug. 2021, [Peer-reviewed]
English, Scientific journal

Artificial Fusion Protein to Facilitate Calcium Carbonate Mineralization on Insoluble Polysaccharide for Efficient Biocementation
Thiloththama H. K. Nawarathna; Kazunori Nakashima; Tetsuya Kawabe; Wilson Mwandira; Kiyofumi Kurumisawa; Satoru Kawasaki
ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 9, 34, 11493, 11502, Aug. 2021, [Peer-reviewed]
English, Scientific journal

Effect of Scallop Powder Addition on MICP Treatment of Amorphous Peat
Sivakumar Gowthaman; Meiqi Chen; Kazunori Nakashima; Satoru Kawasaki
FRONTIERS IN ENVIRONMENTAL SCIENCE, 9, Jul. 2021, [Peer-reviewed]
English, Scientific journal

Bio-Mediated Soil Improvement Using Plant Derived Enzyme in Addition to Magnesium Ion
Md Al Imran; Kazunori Nakashima; Satoru Kawasaki
CRYSTALS, 11, 5, May 2021, [Peer-reviewed]
English, Scientific journal

Shoreline mapping based on beachrocks identification in Krakal-Sadranan beach, Yogyakarta, Indonesia
Lutfian R. Daryono; Kazunori Nakashima; Satoru Kawasaki; Anastasia D. Titisari; Didit H. Barianto; Imam Suyanto; Arief Rahmadi
BULLETIN OF ENGINEERING GEOLOGY AND THE ENVIRONMENT, 80, 3, 2825, 2844, Mar. 2021, [Peer-reviewed]
English, Scientific journal

IMPROVEMENT OF USING CRUDE EXTRACT UREASE FROM WATERMELON SEEDS FOR BIOCEMENTATION TECHNOLOGY
Md Al Imran; Kazunori Nakashima; Niki Evelpidou; Satoru Kawasaki
INTERNATIONAL JOURNAL OF GEOMATE, 20, 78, 142, 147, Feb. 2021, [Peer-reviewed]
English, Scientific journal

EVALUATING MECHANICAL STRENGTH OF PEAT SOIL TREATED BY FIBER INCORPORATED BIO-CEMENTATION
Meiqi Chen; Sivakumar Gowthaman; Kazunori Nakashima; Satoru Kawasaki
INTERNATIONAL JOURNAL OF GEOMATE, 20, 78, 121, 127, Feb. 2021, [Peer-reviewed]
English, Scientific journal

A two-stage treatment process for the management of produced ammonium by-products in ureolytic bio-cementation process
A. Mohsenzadeh; E. Aflaki; S. Gowthaman; K. Nakashima; S. Kawasaki; T. Ebadi
International Journal of Environmental Science and Technology, Springer Science and Business Media LLC, 27 Jan. 2021, [Peer-reviewed]
Scientific journal

Biosorption of Pb (II) and Zn (II) from aqueous solution by Oceanobacillus profundus isolated from an abandoned mine
Wilson Mwandira; Kazunori Nakashima; Satoru Kawasaki; Allison Arabelo; Kawawa Banda; Imasiku Nyambe; Meki Chirwa; Mayumi Ito; Tsutomu Sato; Toshifumi Igarashi; Hokuto Nakata; Shouta Nakayama; Mayumi Ishizuka
Scientific Reports, 10, 1, 21189, Springer Science and Business Media LLC, Dec. 2020, [Peer-reviewed]
Scientific journal, The present study investigated biosorption of Pb (II) and Zn (II) using a heavy metal tolerant bacterium Oceanobacillus profundus KBZ 3-2 isolated from a contaminated site. The effects of process parameters such as effect on bacterial growth, pH and initial lead ion concentration were studied. The results showed that the maximum removal percentage for Pb (II) was 97% at an initial concentration of 50 mg/L whereas maximum removal percentage for Zn (II) was at 54% at an initial concentration of 2 mg/L obtained at pH 6 and 30 °C. The isolated bacteria were found to sequester both Pb (II) and Zn (II) in the extracellular polymeric substance (EPS). The EPS facilitates ion exchange and metal chelation-complexation by virtue of the existence of ionizable functional groups such as carboxyl, sulfate, and phosphate present in the protein and polysaccharides. Therefore, the use of indigenous bacteria in the remediation of contaminated water is an eco-friendly way of solving anthropogenic contamination.

Applications of Chitosan in Environmental Remediation: A Short Review
Preeti Pal; Anjali Pal; Kazunori Nakashima; Brijesh Kumar Yadav
Chemosphere, 266, Nov. 2020, [Peer-reviewed], ［Internationally co-authored］
English

Distribution and cementation characteristics of beachrocks along southern, southwestern and western coast of Sri Lanka
Pahala Ge; Nishadi Nayanthara; Anjula Buddhika; Nayomi Dassanayake; Kazunori Nakashima; Satoru Kawasaki
Journal of Sedimentary Environments, 6, 1, 93, 106, Nov. 2020, [Peer-reviewed]
English, Scientific journal

The Influence of the Addition of Plant-Based Natural Fibers (Jute) on Biocemented Sand Using MICP Method
Md Al Imran; Sivakumar Gowthaman; Kazunori Nakashima; Satoru Kawasaki
Materials, 13, 18, 4198, Sep. 2020, [Peer-reviewed]

Freeze-Thaw Durability and Shear Responses of Cemented Slope Soil Treated by Microbial Induced Carbonate Precipitation
Gowthaman, S; Nakashima, K; Kawasaki, S
Soils and Foundations, 60, 4, 840, 855, Elsevier BV, Aug. 2020, [Peer-reviewed]
Scientific journal

Biological route to fabricate silica on cellulose using immobilized silicatein fused with a carbohydrate-binding module
Kasun Godigamuwa; Kazunori Nakashima; Junnosuke Okamoto; Satoru Kawasaki
Biomacromolecules, American Chemical Society (ACS), 16 Jun. 2020, [Peer-reviewed]
Scientific journal

Cellulose-metallothionein biosorbent for removal of Pb(II) and Zn(II) from polluted water
Wilson Mwandira; Kazunori Nakashima; Yuki Togo; Tsutomu Sato; Satoru Kawasaki
Chemosphere, 246, 125733, May 2020, [Peer-reviewed]
English, Scientific journal

Investigation of natural beachrock and physical-mechanical comparison with artificial beachrock induced by MICP as a protective measure against beach erosion at Yogyakarta, Indonesia
Lutfian R. Daryono; Kazunori Nakashima; Satoru Kawasaki; Koichi Suzuki; Imam Suyanto; Arief Rahmadi
Geosciences, 10, 4, 143, Apr. 2020, [Peer-reviewed]
English, Scientific journal

A novel metal adsorbent composed of hexa-histidine tag and carbohydrate-binding module on cellulose
Yuki Togo; Kazunori Nakashima; Wilson Mwandira; Satoru Kawasaki
Analytical Sciences, 36, 4, 459, 464, Apr. 2020, [Peer-reviewed]
English, Scientific journal

Microbial Leaching of Iron from Hematite: Direct or Indirect Elution
Apichaya Aneksampant; Kazunori Nakashima; Satoru Kawasaki
Materials Transactions, 61, 2, 396, 401, Feb. 2020, [Peer-reviewed]
English, Scientific journal

Sediment characteristics of beachrock: A baseline investigation based on microbial induced carbonate precipitation at Krakal-Sadranan Beach, Yogyakarta, Indonesia
Lutfian Rusdi Daryono; Kazunori Nakashima; Satoru Kawasaki; Anastasia Dewi Titisari; Didit Hadi Barianto
Applied Sciences, 10, 2, 520, Jan. 2020, [Peer-reviewed]
English, Scientific journal

Feasibility study for slope soil stabilization by microbial induced carbonate precipitation (MICP) using indigenous bacteria isolated from cold subarctic region
Sivakumar Gowthaman; Takashi Iki; Kazunori Nakashima; Koji Ebina; Satoru Kawasaki
SN Applied Sciences, 1, 11, 1480, Nov. 2019, [Peer-reviewed]
English, Scientific journal

Feasibility Study of Native Ureolytic Bacteria for Biocementation Towards Coastal Erosion Protection by MICP Method
Md Al Imran; Shuya Kimura; Kazunori Nakashima; Niki Evelpidou; Satoru Kawasaki
Applied Sciences, 9, 20, 4462, Oct. 2019, [Peer-reviewed]

A novel sustainable approach for coastal erosion protection through biocementation
Md Al Imran; Shuya Kimura; Kazunori Nakashima; Niki Evelpidou; Satoru Kawasaki
Applied Sciences, 9, 20, 4462, Oct. 2019, [Peer-reviewed]
English, Scientific journal

Solidification of sand by Pb(II)-tolerant bacteria for capping mine waste to control metallic dust: Case of the abandoned Kabwe Mine, Zambia.
Wilson Mwandira; Kazunori Nakashima; Satoru Kawasaki; Mayumi Ito; Tsutomu Sato; Toshifumi Igarashi; Meki Chirwa; Kawawa Banda; Imasiku Nyambe; Shouta Nakayama; Hokuto Nakata; Mayumi Ishizuka
Chemosphere, 228, 17, 25, Aug. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Environmental impacts resulting from historic lead and zinc mining in Kabwe, Zambia affect human health due to the dust generated from the mine waste that contains lead, a known hazardous pollutant. We employed microbially induced calcium carbonate precipitation (MICP), an alternative capping method, to prevent dust generation and reduce the mobility of contaminants. Pb-resistant Oceanobacillus profundus KBZ 1-3 and O. profundus KBZ 2-5 isolated from Kabwe were used to biocement the sand that would act as a cover to prevent dust and water infiltration. Sand biocemented by KBZ 1-3 and KBZ 2-5 had maximum unconfined compressive strength values of 3.2 MPa and 5.5 MPa, respectively. Additionally, biocemented sand exhibited reduced water permeability values of 9.6 × 10-8 m/s and 8.9 × 10-8 m/s for O. profundus KBZ 1-3 and KBZ 2-5, respectively, which could potentially limit the entrance of water and oxygen into the dump, hence reducing the leaching of heavy metals. We propose that these isolates represent an option for bioremediating contaminated waste by preventing both metallic dust from becoming airborne and rainwater from infiltrating into the waste. O. profundus KBZ 1-3 and O. profundus KBZ 2-5 isolated form Kabwe represent a novel species that has, for the first time, been applied in a bioremediation study.

Efficacy of biocementation of lead mine waste from the Kabwe Mine site evaluated using Pararhodobacter sp.
Wilson Mwandira; Kazunori Nakashima; Satoru Kawasaki; Mayumi Ito; Tsutomu Sato; Toshifumi Igarashi; Kawawa Banda; Meki Chirwa; Imasiku Nyambe; Shouta Nakayama; Mayumi Ishizuka
Environmental science and pollution research international, 26, 15, 15653, 15664, May 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Biocementation of hazardous waste is used in reducing the mobility of contaminants, but studies on evaluating its efficacy have not been well documented. Therefore, to evaluate the efficacy of this method, physicochemical factors affecting stabilized hazardous products of in situ microbially induced calcium carbonate precipitation (MICP) were determined. The strength and leach resistance were investigated using the bacterium Pararhodobacter sp. Pb-contaminated kiln slag (KS) and leach plant residue (LPR) collected from Kabwe, Zambia, were investigated. Biocemented KS and KS/LPR had leachate Pb concentrations below the detection limit of < 0.001 mg/L, resisted slaking, and had maximum unconfined compressive strengths of 8 MPa for KS and 4 MPa for KS/LPR. Furthermore, biocemented KS and KS/LPR exhibited lower water absorption coefficient values, which could potentially reduce the water transportation of Pb2+. The results of this study show that MICP can reduce Pb2+ mobility in mine wastes. The improved physicochemical properties of the biocemented materials, therefore, indicates that this technique is an effective tool in stabilizing hazardous mine wastes and, consequently, preventing water and soil contamination.

Microbial Induced Carbonate Precipitation Using a Native Inland Bacterium for Beach Sand Stabilization in Near Shore Areas
Pahala Ge; Nishadi Nayanthara; Anjula Buddhika; Nayomi Dassanayake; Kazunori Nakashima; Satoru Kawasaki
Applied Sciences, 9, 3201, 2019, [Peer-reviewed]

Biocementation of Sri Lankan beach sand using locally isolated bacteria: A baseline study on the effect of segregated culture media
Pahala Ge; Nishadi Nayanthara; Anjula Buddhika; Nayomi Dassanayake; Kazunori Nakashima; Satoru Kawasaki
International Journal of GEOMATE, 17, 55, 62, 2019, [Peer-reviewed]

Chitosan enhances calcium carbonate precipitation and solidification mediated by bacteria
Thiloththama Hiranya; Kumari Nawarathna; Kazunori Nakashima; Satoru Kawasaki
International Journal of Biological Macromolecules, 133, 867, 874, 2019, [Peer-reviewed]

Microbial induced slope surface stabilization using industrial-grade chemicals: A preliminary laboratory study
Sivakumar Gowthaman; Shumpei Mitsuyama; Kazunori Nakashima; Masahiro Komatsu; Satoru Kawasaki
International Journal of GEOMATE, 17, 110, 116, 2019, [Peer-reviewed]

Factors Affecting the Urease Activity of Native Ureolytic Bacteria Isolated from Coastal Areas
Md Al Imran; Kazunori Nakashima; Niki Evelpidou; Satoru Kawasak
Geomechanics and Engineering, 17, 421, 427, 2019, [Peer-reviewed]

Biogeotechnical Approach for Slope Soil Stabilization Using Locally Isolated Bacteria and Inexpensive Low-grade Chemicals: A Feasibility Study on Hokkaido Expressway Soil, Japan
Sivakumar Gowthaman; Shumpei Mitsuyama; Kazunori Nakashima; Masahiro Komatsu; Satoru Kawasaki
Soils and Foundations, 59, 484, 499, 2019, [Peer-reviewed]

Bio-based cementation using urease-producing bacteria: Sand solidification and kinetics
K. Nakashima; M. Fujita; T. H. K. Nawarathna; S. Kawasaki
New Biotechnology, 44, s154, Oct. 2018, [Peer-reviewed]
English, International conference proceedings

A state-of-the-art review on soil reinforcement technology using natural plant fiber materials: Past findings, present trends and future directions
Sivakumar Gowthaman; Kazunori Nakashima; Satoru Kawasaki
Materials, 11, 4, 553, MDPI AG, 04 Apr. 2018, [Peer-reviewed]
English, Scientific journal

Roles of Microbial Activity and Anthraquinone-2,7-disulfonate as a Model of Humic Substances in Leaching of Iron from Hematite into Seawater
Apichaya Aneksampant; Atsushi Tanaka; Xuefei Tu; Hisanori Iwai; Mitsuo Yamamoto; Kazunori Nakashima; Masami Fukushima
Analytical Sciences, 2018, [Peer-reviewed]
English, Scientific journal

Effects of Various Factors on Carbonate Particle Growth Using Ureolytic Bacteria
Md. Al Imran; Mai Shinmura; Kazunori Nakashima; Satoru Kawasaki
Materials Transactions, 59, 9, 1520, 1527, 2018, [Peer-reviewed]
English, Others

Effects of cationic polypeptide on CaCO3 crystallization and sand solidification by microbial-induced carbonate precipitation
Thiloththama H; K. Nawarathna; Kazunori Nakashima; Masahiro Fujita; Momoko Takatsu; Satoru Kawasaki
ACS Sustainable Chemistry & Engineering, 6, 10315, 10322, 2018, [Peer-reviewed]
English

Soil improvement using plant-derived urease-induced Calcium Carbonate precipitation
R. A. N. Dilrukshi; Kazunori Nakashima; Satoru Kawasaki
Soils and Foundations, 58, 894, 910, 2018, [Peer-reviewed]
English, Scientific journal

Enhancement of microbially induced carbonate precipitation using organic biopolymer
Thiloththama Hiranya Kumari Nawarathna; Kazunori Nakashima; Satoru Kawasaki
International Journal of GEOMATE, 14, 41, 7, 12, GEOMATE International Society, 2018, [Peer-reviewed]
English, Scientific journal

Electricity generation from rice bran by a microbial fuel cell and the influence of hydrodynamic cavitation pretreatment
Yuta Yoshimura; Kazunori Nakashima; Masaji Kato; Kengo Inoue; Fumiyoshi Okazaki; Hitoshi Soyama; Satoru Kawasaki
ACS Omega, 3, 11, 15267, 15271, 2018, [Peer-reviewed]
English, Scientific journal

Bioremediation of lead-contaminated mine waste by Pararhodobacter sp based on the microbially induced calcium carbonate precipitation technique and its effects on strength of coarse and fine grained sand
Wilson Mwandira; Kazunori Nakashima; Satoru Kawasaki
ECOLOGICAL ENGINEERING, 109, A, 57, 64, Dec. 2017, [Peer-reviewed]
English, Scientific journal

Whole-cell evaluation of urease activity of Pararhodobacter sp isolated from peripheral beachrock
Masahiro Fujita; Kazunori Nakashima; Varenyam Achal; Satoru Kawasaki
BIOCHEMICAL ENGINEERING JOURNAL, 124, 1, 5, Aug. 2017, [Peer-reviewed]
English, Scientific journal

Direct Ethanol Production from Ionic Liquid-Pretreated Lignocellulosic Biomass by Cellulase-Displaying Yeasts
Ryosuke Yamada; Kazunori Nakashima; Nanami Asai-Nakashima; Wataru Tokuhara; Nobuhiro Ishida; Satoshi Katahira; Noriho Kamiya; Chiaki Ogino; Akihiko Kondo
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 182, 1, 229, 237, May 2017, [Peer-reviewed]
English, Scientific journal

Combination technology of geotextile tube and artificial beachrock for coastal protection
Md. Al Imran; Kazunori Nakashima; Satoru Kawasaki
International Journal of GEOMATE, 13, 39, 67, 72, GEOMATE International Society, 2017, [Peer-reviewed]
English, Scientific journal

Evaluation of porosity in biogrouted sand using microfocus X-ray CT
Shumpei Mitsuyama; Kazunori Nakashima; Satoru Kawasaki
International Journal of GEOMATE, 12, 31, 71, 76, GEOMATE International Society, 2017, [Peer-reviewed]
English, Scientific journal

Effectiveness of Adjustable-Volume Packed-Bed Reactor with an Ion-Exchange Resin Catalyst for Continuous Production
Kota Yamazaki; Naomi Shibasaki-Kitakawa; Kazunori Nakashima; Toshikuni Yonemoto
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 49, 7, 668, 672, Jul. 2016, [Peer-reviewed]
English, Scientific journal

Pretreatment combining ultrasound and sodium percarbonate under mild conditions for efficient degradation of corn stover
Kazunori Nakashima; Yuuki Ebi; Masaki Kubo; Naomi Shibasaki-Kitakawa; Toshikuni Yonemoto
ULTRASONICS SONOCHEMISTRY, 29, 455, 460, Mar. 2016, [Peer-reviewed]
English, Scientific journal

Novel simple process for tocopherols selective recovery from vegetable oils by adsorption and desorption with an anion-exchange resin
Kousuke Hiromori; Naomi Shibasaki-Kitakawa; Kazunori Nakashima; Toshikuni Yonemoto
FOOD CHEMISTRY, 194, 1, 5, Mar. 2016, [Peer-reviewed]
English, Scientific journal

Hydrodynamic Cavitation Reactor for Efficient Pretreatment of Lignocellulosic Biomass
Kazunori Nakashima; Yuuki Ebi; Naomi Shibasaki-Kitakawa; Hitoshi Soyama; Toshikuni Yonemoto
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 55, 7, 1866, 1871, Feb. 2016, [Peer-reviewed]
English, Scientific journal

Production of high quality biodiesel from waste acid oil obtained during edible oil refining using ion-exchange resin catalysts
Naomi Shibasaki-Kitakawa; Kousuke Hiromori; Toru Ihara; Kazunori Nakashima; Toshikuni Yonemoto
FUEL, 139, 11, 17, Jan. 2015
English, Scientific journal

Improvement of enzymatic activity of beta-glucosidase from Thermotoga maritima by 1-butyl-3-methylimidazolium acetate
Motonori Kudou; Yuka Kubota; Nanami Nakashima; Fumiyoshi Okazaki; Kazunori Nakashima; Chiaki Ogino; Akihiko Kondo
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 104, 17, 22, Jun. 2014
English, Scientific journal

A fusion enzyme consisting of bacterial expansin and endoglucanase for the degradation of highly crystalline cellulose
Kazunori Nakashima; Koji Endo; Naomi Shibasaki-Kitakawa; Toshikuni Yonemoto
RSC ADVANCES, 4, 83, 43815, 43820, 2014
English, Scientific journal

Thermal Stability and Starch Degradation Profile of alpha-Amylase from Streptomyces avermitilis
Sang Youn Hwang; Kazunori Nakashima; Naoko Okai; Fumiyoshi Okazaki; Michiru Miyake; Koichi Harazono; Chiaki Ogino; Akihiko Kondo
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 77, 12, 2449, 2453, Dec. 2013
English, Scientific journal

Simultaneous production of high quality biodiesel and glycerin from Jatropha oil using ion-exchange resins as catalysts and adsorbent
Naomi Shibasaki-Kitakawa; Keiichi Kanagawa; Kazunori Nakashima; Toshikuni Yonemoto
BIORESOURCE TECHNOLOGY, 142, 732, 736, Aug. 2013
English, Scientific journal

Production of human secreted alkaline phosphatase in suspension and immobilization cultures of tobacco NT1 cell
Kazunori Nakashima; Naomi Shibasaki-Kitakawa; Takuya Miyamoto; Masaki Kubo; Toshikuni Yonemoto; Michael L. Shuler
BIOCHEMICAL ENGINEERING JOURNAL, 77, 177, 182, Aug. 2013
English, Scientific journal

Production of biodiesel from plant oil hydrolysates using an Aspergillus oryzae whole-cell biocatalyst highly expressing Candida antarctica lipase B
Daisuke Adachi; Shinji Hama; Kazunori Nakashima; Takayuki Bogaki; Chiaki Ogino; Akihiko Kondo
BIORESOURCE TECHNOLOGY, 135, 410, 416, May 2013
English, Scientific journal

An integrative process model of enzymatic biodiesel production through ethanol fermentation of brown rice followed by lipase-catalyzed ethanolysis in a water-containing system
Daisuke Adachi; Risa Koda; Shinji Hama; Ryosuke Yamada; Kazunori Nakashima; Chiaki Ogino; Akihiko Kondo
ENZYME AND MICROBIAL TECHNOLOGY, 52, 2, 118, 122, Feb. 2013
English, Scientific journal

Versatile Supramolecular Gelators That Can Harden Water, Organic Solvents and Ionic Liquids
Nami Minakuchi; Kazuki Hoe; Daisuke Yamaki; Seiichiro Ten-no; Kazunori Nakashima; Masahiro Goto; Minoru Mizuhata; Tatsuo Maruyama
LANGMUIR, 28, 25, 9259, 9266, Jun. 2012
English, Scientific journal

Short time ionic liquids pretreatment on lignocellulosic biomass to enhance enzymatic saccharification
Uju; Yasuhiro Shoda; Aya Nakamoto; Masahiro Goto; Wataru Tokuhara; Yoshiyuki Noritake; Satoshi Katahira; Nobuhiro Ishida; Kazunori Nakashima; Chiaki Ogino; Noriho Kamiya
BIORESOURCE TECHNOLOGY, 103, 1, 446, 452, Jan. 2012
English, Scientific journal

Development of an Aspergillus oryzae whole-cell biocatalyst coexpressing triglyceride and partial glyceride lipases for biodiesel production
Daisuke Adachi; Shinji Hama; Takao Numata; Kazunori Nakashima; Chiaki Ogino; Hideki Fukuda; Akihiko Kondo
BIORESOURCE TECHNOLOGY, 102, 12, 6723, 6729, Jun. 2011
English, Scientific journal

Highly efficient biodiesel production by a whole-cell biocatalyst employing a system with high lipase expression in Aspergillus oryzae
Tomohiro Takaya; Risa Koda; Daisuke Adachi; Kazunori Nakashima; Junpei Wada; Takayuki Bogaki; Chiaki Ogino; Akihiko Kondo
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90, 3, 1171, 1177, May 2011
English, Scientific journal

Water activity dependence of performance of surface-displayed lipase in yeast cells: A unique water requirement for enzymatic synthetic reaction in organic media
Ayumi Yoshida; Shinji Hama; Kazunori Nakashima; Akihiko Kondo
ENZYME AND MICROBIAL TECHNOLOGY, 48, 4-5, 334, 338, Apr. 2011
English, Scientific journal

Direct bioethanol production from cellulose by the combination of cellulase-displaying yeast and ionic liquid pretreatment
Kazunori Nakashima; Koji Yamaguchi; Naho Taniguchi; Shogo Arai; Ryosuke Yamada; Satoshi Katahira; Nobuhiro Ishida; Haruo Takahashi; Chiaki Ogino; Akihiko Kondo
GREEN CHEMISTRY, 13, 10, 2948, 2953, 2011
English, Scientific journal

Ethanolysis of rapeseed oil to produce biodiesel fuel catalyzed by Fusarium heterosporum lipase-expressing fungus immobilized whole-cell biocatalysts
Risa Koda; Takao Numata; Shinji Hama; Sriappareddy Tamalampudi; Kazunori Nakashima; Tsutomu Tanaka; Chiaki Ogino; Hideki Fukuda; Akihiko Kondo
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 66, 1-2, 101, 104, Sep. 2010
English, Scientific journal

Surfactant-modified yeast whole-cell biocatalyst displaying lipase on cell surface for enzymatic production of structured lipids in organic media
Shinji Hama; Ayumi Yoshida; Kazunori Nakashima; Hideo Noda; Hideki Fukuda; Akihiko Kondo
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87, 2, 537, 543, Jun. 2010
English, Scientific journal

Recent advances of enzymatic reactions in ionic liquids
Muhammad Moniruzzaman; Kazunori Nakashima; Noriho Kamiya; Masahiro Goto
BIOCHEMICAL ENGINEERING JOURNAL, 48, 3, 295, 314, Feb. 2010
English

Proteinase-mediated drastic morphological change of peptide-amphiphile to induce supramolecular hydrogelation
Daisuke Koda; Tatsuo Maruyama; Nami Minakuchi; Kazunori Nakashima; Masahiro Goto
CHEMICAL COMMUNICATIONS, 46, 6, 979, 981, 2010
English, Scientific journal

Production of biodiesel fuel from soybean oil catalyzed by fungus whole-cell biocatalysts in ionic liquids
Shogo Arai; Kazunori Nakashima; Takanori Tanino; Chiaki Ogino; Akihiko Kondo; Hideki Fukuda
ENZYME AND MICROBIAL TECHNOLOGY, 46, 1, 51, 55, Jan. 2010
English, Scientific journal

Enzyme encapsulation in microparticles composed of polymerized ionic liquids for highly active and reusable biocatalysts
Kazunori Nakashima; Noriho Kamiya; Daisuke Koda; Tatsuo Maruyama; Masahiro Goto
ORGANIC & BIOMOLECULAR CHEMISTRY, 7, 11, 2353, 2358, 2009
English, Scientific journal

Extraction Behavior of Indium with TOPO into Ionic Liquids
Fukiko Kubota; Yosuke Shimobori; Yusuke Koyanagi; Kazunori Nakashima; Kojiro Shimojo; Noriho Kamiya; Masahiro Goto
SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN, 16, 151, 155, 2009
English, Scientific journal

Metal Extraction from Water and Organic Solvents into Fluorous Solvents by Fluorinated beta-Diketone and Its Application to the Colorimetric Analysis of Metal Ions
Kazunori Nakashima; Tatsuo Maruyama; Fukiko Kubota; Masahiro Goto
ANALYTICAL SCIENCES, 25, 1, 77, 82, Jan. 2009
English, Scientific journal

Enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media
Noriho Kamiya; Yuichi Matsushita; Misa Hanaki; Kazunori Nakashima; Mamiko Narita; Masahiro Goto; Haruo Takahashi
BIOTECHNOLOGY LETTERS, 30, 6, 1037, 1040, Jun. 2008
English, Scientific journal

Formation of reverse micelles in a room-temperature ionic liquid
Muhammad Moniruzzaman; Kamiya Noriho; Kazunori Nakashima; Masahiro Goto
CHEMPHYSCHEM, 9, 5, 689, 692, Apr. 2008
English, Scientific journal

CO(2) separation facilitated by task-specific ionic liquids using a supported liquid membrane
Shoji Hanioka; Tatsuo Maruyama; Tomohiro Sotani; Masahiro Teramoto; Hideto Matsuyama; Kazunori Nakashima; Misa Hanaki; Fukiko Kubota; Masahiro Goto
JOURNAL OF MEMBRANE SCIENCE, 314, 1-2, 1, 4, Apr. 2008
English, Scientific journal

A rapid assay of protease activity in ionic liquids using chromogenic substrates
Kazunori Nakashima; Tatsuo Maruyama; Masahiro Goto; Noriho Kamiya
Analytical Biochemistry, Vol. 374 Issue: 2 Pages: 2, Mar. 2008, [Peer-reviewed]
English, Scientific journal

Spectrophotometric assay for protease activity in ionic liquids using chromogenic substrates
Kazunorl Nakashima; Tatsuo Maruyama; Noriho Kamiya; Masahiro Goto
ANALYTICAL BIOCHEMISTRY, 374, 2, 285, 290, Mar. 2008
English, Scientific journal

Extraction of lanthanide ions with an organophosphorous extractant into ionic liquids
Fukiko Kubota; Yusuke Koyanagi; Kazunori Nakashima; Kojiro Shimojo; Noriho Kamiya; Masahiro Goto
SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN, 15, 81, 87, 2008
English, Scientific journal

Water-in-ionic liquid microemulsions as a new medium for enzymatic reactions
Muhammad Moniruzzaman; Noriho Kamiya; Kazunori Nakashima; Masahiro Goto
GREEN CHEMISTRY, 10, 5, 497, 500, 2008
English, Scientific journal

Perfluorocarbon-based liquid-liquid extraction for separation of transition metal ions
Tatsuo Maruyama; Kazunori Nakashima; Fukiko Kubota; Masahiro Goto
ANALYTICAL SCIENCES, 23, 7, 763, 765, Jul. 2007
English, Scientific journal

Extraction of cytochrome c by a functionalized ionic liquid containing a crown ether
Fukiko Kubota; Yusuke Koyanagi; Kazunori Nakashima; Kojiro Shimojo; Noriho Kamiya; Masahiro Goto
SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN, 14, 115, 120, 2007
English, Scientific journal

Activation of lipase in ionic liquids by modification with comb-shaped poly(ethylene glycol)
Kazunori Nakashima; Jun Okada; Tatsuo Maruyama; Noriho Kamiya; Masahiro Goto
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS, 7, 7, 692, 698, Oct. 2006
English, Scientific journal

Homogeneous enzymatic reactions in ionic liquids with poly(ethylene glycol)modified subtilisin
Kazunori Nakashima; Tatsuo Maruyama; Noriho Kamiya; Masahiro Goto
ORGANIC & BIOMOLECULAR CHEMISTRY, 4, 18, 3462, 3467, 2006
English, Scientific journal

Crown ether-mediated extraction and functional conversion of cytochrome c in ionic liquids
K Shimojo; K Nakashima; N Kamiya; M Goto
BIOMACROMOLECULES, 7, 1, 2, 5, Jan. 2006
English, Scientific journal

Feasibility of ionic liquids as alternative separation media for industrial solvent extraction processes
K Nakashima; F Kubota; T Maruyama; M Goto
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 44, 12, 4368, 4372, Jun. 2005
English, Scientific journal

Comb-shaped poly(ethylene glycol)-modified subtilisin Carlsberg is soluble and highly active in ionic liquids
K Nakashima; T Maruyama; N Kamiya; M Goto
CHEMICAL COMMUNICATIONS, 34, 4297, 4299, 2005
English, Scientific journal

Ionic liquids as a novel solvent for lanthanide extraction
K Nakashima; F Kubota; T Maruyama; M Goto
ANALYTICAL SCIENCES, 19, 8, 1097, 1098, Aug. 2003
English, Scientific journal

Extraction of Amino Acids by Calixarenes in An Aliphatic Organic Solvent
K. Nakashima; T. Oshima; M. Goto
Solvent Extraction Research and Development, Japan, 9, 69, 79, Japanese Association of Solvent Extraction, 2002
English

Engineered Escherichia coli cell capable of specific binding to metal surface
K. Nakashima; Y. Iwata; S. Kawasaki, ACS Fall 2019 National Meeting & Exposition, San Diego, United States, Aug. 2019
English

Microbially induced carbonate precipitation assisted by poly-L-lysine: ecofriendly approach inspired by nature
T.H.K. Nawarathna; K. Nakashima; S. Kawasaki, ACS Fall 2019 National Meeting & Exposition, San Diego, United States, Aug. 2019
English

Beachrock identification using geology and geophysical approaches in Indonesia
Daryono L. R; Nakashima K; Kawasaki S; Titisari A. D; Barianto D. H; Suyanto I; Rahmadi A, Proceedings of International Symposium on Earth Resources Management and Environment, ISERME 2019, 51, 59, Aug. 2019
English

Zinc and Lead Biosorption by Oceanobacillus Profundus Kbz 3-2: a Bacterial Strain Resistant to Metals Isolated from Metal polluted Soils in Kabwe, Zambia
Wilson Mwandira; Kazunori Nakashima; Satoru Kawasaki; Mayumi Ito; Tsutomu Sato; Toshifumi Igarashi; Meki Chirwa; Kawawa Banda; Imasiku Nyambe; Shouta Nakayama; Mayumi Ishizuka, Proceedings of the 14th Asian Congress on Biotechnology (ACB2019), Jul. 2019
English

Biological Control of Filament Formation Composed of Silica-polymerizing Enzymes
Kazunori Nakashima; Junnosuke Okamoto; Satoru Kawasaki, Proceedings of the 14th Asian Congress on Biotechnology (ACB2019), Jul. 2019
English

Bio-cementation for Slope Soil Stabilization againstSurface Erosion: A Bench-scale Preliminary Investigation
S. Gowthaman; K. Nakashima; K. Ebina; S. Kawasaki, Proceedings of the 53rd US Rock Mechanics/Geomechanics Symposium, Jun. 2019
English

Development of small scale biocemented rocks based on natural beachrock cement characteristics as a novel approach towards coastal erosion mitigation
P. G. N. Nayanthara; A. B; N. Dassanayake; K. Nakashima; S. Kawasaki, Proceedings of the 53rd US Rock Mechanics / Geomechanics Symposium, Jun. 2019
English

Evaluating possible avenues for soil improvement by biocementation in Sri Lanka: A preliminary investigation
P. G. N. Nayanthara; A. B. N; Dassanayake; kazunori Nakashima; Satoru Kawasaki, Proceedings of the Eighth International Conference of Geotechnique, Construction Materials and Environment (GEOMATE 2018), 759, 764, Nov. 2018
English

Bio-inspired stabilization of embankment soil mediating Psychrobacillus sp. and low-grade chemicals: Preliminary laboratory investigation
Sivakumar Gowthaman; Shumpei Mitsuyama; Kazunori Nakashima; Masahiro Komatsu; Satoru Kawasaki, Proceedings of the Eighth International Conference of Geotechnique, Construction Materials and Environment (GEOMATE 2018), 163, 168, Nov. 2018
English

Experimental optimization of biocement formation: Alternative countermeasure for surface erosion of cut slope
Gowthaman S; Nakashima K; Ebina K; Kawasaki S, Proceedings of International Symposium on Earth Resources Management and Environment, ISERME 2018, 97, 103, Aug. 2018, [Peer-reviewed]
English

Applicability of coastal ureolytic bacteria to coastal protection in Greece
Md. Al Imran; Kazunori Nakashima; Niki Evelpidou; Satoru Kawasaki, 2018 International Conference on Geomechanics and Engineering (ICGE18), Aug. 2018, [Peer-reviewed]
English

A preliminary investigation on isolation and identification of marine bacteria for biocementation in nearshore environments
Nayanthara P. G. N; Dassanayake A. B. N; Nakashima K; Kawasaki S, Proceedings of International Symposium on Earth Resources Management and Environment, ISERME 2018, 111, 118, Aug. 2018
English

Microbially induced carbonate precipitation for expressway slope protection
Mitsuyama, S; Nakashima, K; Komatsu, M; Kawasaki, S, Abstract Proceedings of the 1st International KAMPAI Symposium on Sustainable Management of Resources and Environment in the 21st Century, 23, Nov. 2017
English

Effect of cationic bio-polymer on CaCO3 crystallization by microbe
T.H.K. Nawarathna; Kazunori Nakashima; Satoru Kawasaki, Abstract Proceedings of the 1st International KAMPAI Symposium on Sustainable Management of Resources and Environment in the 21st Century, 24, Nov. 2017
English

Immobilization of Lead-Contaminated Mine Waste by Pararhodobacter sp.
Mwandira, W; Nakashima, K; Kawasaki, S; Chirwa, M; Banda, K.E; Nyambe, I.A, Abstract Proceedings of the 1st International KAMPAI Symposium on Sustainable Management of Resources and Environment in the 21st Century, 39, Nov. 2017
English

Effect of Organic biopolymer on the microbially induced carbonate precipitation and its morphology
Thiloththama Hiranya; Kumari Nawarathna; Kazunori Nakashima; Satoru Kawasaki, Proceedings of the Seventh International Conference of Geotechnique, Construction Materials and Environment (GEOMATE 2017), 7, 1, 719, 724, Nov. 2017
English

Bioremediation of lead-contaminated mine waste using microbially induced carbonate precipitation
Wilson Mwandira; Kazunori Nakashima; Satoru Kawasaki, Proceedings of International Symposium on Earth Resources Management and Environment, ISERME 2017, 21, 25, Aug. 2017
English

Efect of organic bio-polymer on bio-mineralization of CaCO3
T.H.K. Hiranya; Kazunori Nakashima; Satoru Kawasaki, Proceedings of International Symposium on Earth Resources Management and Environment, ISERME 2017, 59, 66, Aug. 2017
English

Solubilization and functional evaluation of silica-polymerizing enzyme
Hidetoshi Oguri; Kazunori Nakashima; Satoru Kawasaki, The 13th the Asian Congress on Biotechnology 2017 (ACB2017), Jul. 2017
English

Evaluation of porosity in biogrouted sand using microfocus X-ray CT
Shumpei Mitsuyama; Kazunori Nakashima; Satoru Kawasaki, Proceedings of the Sixth International Conference of Geotechnique, Construction Materials and Environment (GEOMATE 2016), 145, 150, Nov. 2016
English

Pilot-Scale Production of High Quality Biodiesel from Vegetable Oils with High Free Fatty Acid Content using Ion-Exchange Resin Catalysts
Naomi Shibasaki-Kitakawa; Tomokatsu Oyanagi; Siqingaowa Borjigin; Kazunori Nakashima; Tadao Niwa; Ichiro Minami; Toshikuni Yonemoto, KAGAKU KOGAKU RONBUNSHU, 42, 1, 30, 36, 2016, [Peer-reviewed]
Japanese

3P-023 Soluble expression of silicatein in Escherichia coli using Mistic protein from Bacillus subtilis
Hasegawa Rin; Nakashima Kazunori; Shibasaki-Kitakawa Naomi; Yonemoto Toshikuni, 日本生物工学会大会講演要旨集, 67, 276, 276, 2015
日本生物工学会, Japanese

2P-047 Construction and Degradation Properties of Fusion Enzymes Composed of Bacterial Expansin and Endoglucanase
Nakashima Kazunori; Endo Koji; Shibasaki-Kitakawa Naomi; Yonemoto Toshikuni, 日本生物工学会大会講演要旨集, 66, 118, 118, 2014
日本生物工学会, Japanese

3-11-3 Pilot Plant Production of High Quality Biodiesel Using Ion-exchange Resin Catalysts
Kitakawa Naomi; Oyanagi Tomokatsu; Siqingaowa Borjigin; Nakashima Kazunori; Yonemoto Toshikuni, Proceedings of the Annual Conference of The Japan Institute of Energy, 23, 148, 149, 2014
This study was aimed at the pilot plant production of high quality biodiesel, which fully satisfies fuel specifications, from the cheaper non-edible oil with high free fatty acid content. Not only the fatty acid methyl ester (FAME), but also the fatty acid ethyl ester (FAEE), received much attention as more environmentally preferable biodiesel, was produced by the plant. Both biodiesel products were fully met the fuel standard specifications without any downstream purification processes except for removing alcohol. The calorific values of FAEE were higher than those of FAME and this meant that the fuel economy and horse power of FAEE will be improved., The Japan Institute of Energy, Japanese

脂肪酸100%の油をも原料利用可能な高品質バイオディーゼル連続製造プロセスの開発
北川尚美; 中島一紀; 米本年邦, ケミカルエンジニアリング,化学工業社, 59, 2, 112, 118, 2014

脂肪酸100％の油をも原料利用可能な高品質バイオディーゼル連続製造プロセスの開発
北川尚美; 中島一紀; 米本年邦, ケミカルエンジニヤリング, 59, 2, 20, 26, 2014

脂肪酸100％の油をも原料利用可能な高品質バイオディーゼル連続製造プロセスの開発
北川尚美; 中島一紀; 米本年邦, ケミカルエンジニヤリング, 59, 2, 20, 26, 2014

金属イオンを接着剤として作られる中空タンパク質粒子
中島一紀, 生物工学会誌, 91巻, 5号, 258, 2013

金属イオンを接着剤として作られる中空タンパク質粒子
中島一紀, 生物工学会誌, 91巻, 5号, 258, 258, 2013
日本生物工学会, Japanese

幅広い溶媒をゲル化可能な低分子ゲル化剤
水口奈美; 宝得一貴; 山木大輔; 天能精一郎; 中島一紀; 後藤雅宏; 水畑穣; 丸山達生, 高分子学会予稿集(CD-ROM), 61, 1, 2012

4Ia01 Ultrasonic irradiation for the pretreatment of cellulose for efficient enzymatic hydrolysis
Nakashima Kazunori; Sato Takumi; Kitakawa Naomi; Yonemoto Toshikuni, 日本生物工学会大会講演要旨集, 64, 239, 239, 2012
日本生物工学会, Japanese

イオン液体耐性セルラーゼの単離と酵素特性解析
窪田有華; 工藤基徳; 仲島菜々実; 中島一紀; 岡崎文美; 荻野千秋; 近藤昭彦, 酵素工学研究会講演会講演要旨集, 68th, 2012

イオン液体耐性セルラーゼの探索と機能解析
窪田有華; 山口剛示; 仲島菜々実; 工藤基徳; 中島一紀; 岡崎文美; 荻野千秋; 近藤昭彦, 日本生物工学会大会講演要旨集, 64th, 2012

1Ap14 Development of ionic liquid-based consolidated bioprocessing (i-CBP)
OGINO Chiaki; NAKASHIMA Kazunori; YAMAGUCHI Koji; KUBOTA Yuka; KATAHIRA Satoshi; ISHIDA Nobuhiro; TOKUHARA Wataru; NORITAKE Yoshiyuki; KONDO Akihiko, 日本生物工学会大会講演要旨集, 63, 24, 24, 2011
日本生物工学会, Japanese

1Ap13 Ethanol production from ionic liquid pretreated biomass with cellulase expressing yeast
Katahira Satoshi; Nakamura Risa; Tokuhara Wataru; Noritake Yoshiyuki; Nakashima Kazunori; Kamiya Noriho; Ogino Chiaki; Ishida Nobuhiro, 日本生物工学会大会講演要旨集, 63, 23, 23, 2011
日本生物工学会, Japanese

水・有機溶媒・食用油・イオン液体をゲル化可能な新規低分子ゲル化剤の開発
水口 奈美; 宝得 一貴; 丸山 達生; 大向 吉景; 中島 一紀; 後藤 雅宏; 松山 秀人, 化学工学会 研究発表講演要旨集, 2010, 1088, 1088, 2010
公益社団法人 化学工学会, Japanese

高効率バイオディーゼル生産を目的としたWhole-cell biocatalystによる新規リパーゼ複合系の開発
足立 大輔; 中島 一紀; 荻野 千秋; 近藤 昭彦, 化学工学会 研究発表講演要旨集, 2010, 1008, 1008, 2010
公益社団法人 化学工学会, Japanese

3P-2043 Development of ionic liquid-based consolidated bioprocessing (i-CBP), part. 3.
YAMAGUCHI Koji; NAKASHIMA Kazunori; YAMADA Ryosuke; OGINO Chiaki; KONDO Akihiko, 日本生物工学会大会講演要旨集, 22, 154, 154, 2010
日本生物工学会, Japanese

バイオリファイナリー実現に向けたセルロース分解酵素の新たな選抜法
中島一紀, 化学工学, 74, 5, 248, 2010

次世代燃料・化成品原料製造に向けたバイオリファイナリー戦略
近藤昭彦; 荻野千秋; 蓮沼誠久; 田中勉; 中島一紀, 生物工学会誌, 88, 7, 333, 335, 2010

バイオマスからのバイオ燃料生産に向けた戦略
近藤昭彦; 荻野千秋; 蓮沼誠久; 田中勉; 中島一紀, 月刊バイオインダストリー シーエムシー出版, 27, 11, 6, 12, 2010

バイオリファイナリー実現に向けたセルロース分解酵素の新たな選抜法
中島一紀, 化学工学, 74, 5, 248, 2010

次世代燃料・化成品原料製造に向けたバイオリファイナリー戦略
近藤昭彦; 荻野千秋; 蓮沼誠久; 田中勉; 中島一紀, 生物工学会誌, 88, 7, 333, 335, 2010

バイオマスからのバイオ燃料生産に向けた戦略
近藤昭彦; 荻野千秋; 蓮沼誠久; 田中勉; 中島一紀, 月刊バイオインダストリー,シーエムシー出版, 27, 11, 6, 12, 2010

カルボン酸系新規抽出剤による希土類金属のイオン液体抽出
久保田 富生子; 小柳 裕介; 下条 晃司郎; 中島 一紀; 神谷 典穂; 後藤 雅宏, 化学工学会 研究発表講演要旨集, 2009, 230, 230, 2009
公益社団法人 化学工学会, Japanese

疾病関連生体触媒に応答した超分子ハイドロゲル形成
香田 大輔; 丸山 達生; 中島 一紀; 神谷 典穂; 後藤 雅宏, 化学工学会 研究発表講演要旨集, 2009, 603, 603, 2009
公益社団法人 化学工学会, Japanese

3S1a03 Ionic liquid-based in situ pretreatment and enzymatic saccharification of cellulose
KAMIYA Noriho; MATSUSHITA Yuichi; UEDA Misa; NAKASHIMA Kazunori; GOTO Masahiro; JO Sung-jin; ISHIDA Nobuhiro; TAKAHASHI Haruo, 日本生物工学会大会講演要旨集, 21, 200, 200, 2009
日本生物工学会, Japanese

菌体触媒を用いたバイオディーゼル生産におけるイオン液体の利用
中島 一紀; 荒井 章吾; 谷野 孝徳; 荻野 千秋; 近藤 昭彦; 福田 秀樹, 化学工学会 研究発表講演要旨集, 2009, 912, 912, 2009
公益社団法人 化学工学会, Japanese

プロテアーゼに応答するペプチド性超分子ゲル化剤の創製
香田 大輔; 丸山 達生; 中島 一紀; 後藤 雅宏, 化学工学会 研究発表講演要旨集, 2008, 946, 946, 2008
公益社団法人 化学工学会, Japanese

水-イオン液体混合溶媒中でのセルロースの酵素触媒的分解
松下 雄一; 植田 美紗; 中島 一紀; 神谷 典穂; 後藤 雅宏; 成田 麻美子; 石田 亘広; 高橋 治雄, 化学工学会 研究発表講演要旨集, 2008, 989, 989, 2008
公益社団法人 化学工学会, Japanese

2Ga06 Preparation of Novel Biocomposite Material Based on Ionic Liquid Gel
Nakashima Kazunori; Maruyama Tatsuo; Kamiya Noriho; Goto Masahiro, 日本生物工学会大会講演要旨集, 20, 177, 177, 2008
日本生物工学会, Japanese

2Gp15 Pretreatment and enzymatic saccharification of cellulose using ionic liquids
KAMIYA Noriho; MATSUSHITA Yuichi; UEDA Misa; NAKASHIMA Kazunori; GOTO Masahiro; NARITA Mamiko; ISHIDA Nobuhiro; TAKAHASHI Haruo, 日本生物工学会大会講演要旨集, 20, 183, 183, 2008
日本生物工学会, Japanese

イオン液体からなるゲルへの酵素の封入とその反応特性
中島 一紀; 丸山 達生; 神谷 典穂; 後藤 雅宏, 化学工学会 研究発表講演要旨集, 2008, 424, 424, 2008
公益社団法人 化学工学会, Japanese

イオン液体を媒体とした希土類金属の抽出挙動の検討
小柳 裕介; 久保田 富生子; 中島 一紀; 下条 晃司郎; 神谷 典穂; 後藤 雅宏, 化学工学会 研究発表講演要旨集, 2008, 104, 104, 2008
公益社団法人 化学工学会, Japanese

Formation of Nanoemulsions in a Room-Temperature Ionic Liquid
Muhammad Moniruzzaman, 化学工学会 研究発表講演要旨集, 2008, 599, 599, 2008
The Society of Chemical Engineers, Japan

新たな非水溶媒イオン液体中での酵素反応
中島一紀; 神谷典穂; 後藤雅宏, 月刊バイオインダストリー シーエムシー出版, 25, 7, 24, 34, 2008

新たな非水溶媒イオン液体中での酵素反応
中島一紀; 神谷典穂; 後藤雅宏, 月刊バイオインダストリー,シーエムシー出版, 25, 7, 24, 34, 2008
シーエムシー出版, Japanese

イオン液体を用いた促進輸送膜によるCO2分離
埴岡 昌司; 曽谷 知弘; 寺本 正明; 松山 秀人; 後藤 雅宏; 久保田 富生子; 花木 美紗; 中島 一紀, 化学工学会 研究発表講演要旨集, 2007, 777, 777, 2007
公益社団法人 化学工学会, Japanese

ヒスチジン含有ペプチド性低分子ハイドロゲル化剤の創製と脂肪酸鎖の影響の評価
香田 大輔; 丸山 達生; 園川 沙織; 中島 一紀; 後藤 雅宏, 化学工学会 研究発表講演要旨集, 2007, 832, 832, 2007
公益社団法人 化学工学会, Japanese

酵素を封入したイオン液体ゲルの創製とその触媒特性
中島 一紀; 丸山 達生; 神谷 典穂; 後藤 雅宏, 化学工学会 研究発表講演要旨集, 2007, 833, 833, 2007
公益社団法人 化学工学会, Japanese

イオン液体を用いた生体分子の抽出システムの開発
小柳 裕介; 久保田 富生子; 中島 一紀; 下条 晃司郎; 神谷 典穂; 後藤 雅宏, 化学工学会 研究発表講演要旨集, 2007, 658, 658, 2007
公益社団法人 化学工学会, Japanese

イオン液体を用いた新規プロテアーゼ活性検出システムの開発
中島 一紀; 丸山 達生; 神谷 典穂; 後藤 雅宏, 化学工学会 研究発表講演要旨集, 2007, 404, 404, 2007
公益社団法人 化学工学会, Japanese

Solubilization and functional changes of biomolecules in ionic liquids
Masahiro Goto; Kojiro Shimojo; Kazunori Nakashima, Polymer Preprints, Japan, 55, 4176, 4177, 01 Dec. 2006

生体触媒の新しい反応場としてのイオン液体 －水中から有機溶媒へ、そして今イオン液体へ－
中島一紀; 後藤雅宏, 化学工学, 70, 2, 111, 113, 2006

生体触媒の新しい反応場としてのイオン液体 －水中から有機溶媒へ、そして今イオン液体へ－
中島一紀; 後藤雅宏, 化学工学, 70, 2, 111, 113, 2006

Comb-shaped polyethylene glycol-modifled subtilisin soluble and highly active in ionic liquids
Masahiro Goto; Kazunori Nakashima, AIChE Annual Meeting, Conference Proceedings, 14358, 01 Dec. 2005

Solubilization and high activity of enzymes in ionic liquids by modification with comb-shaped poly (ethylene glycol)
Kazunori Nakashima; Tatsuo Maruyama; Noriho Kamiya; Masahiro Goto, Polymer Preprints, Japan, 54, 4647, 4648, 01 Dec. 2005

1C14-4 Solubilization and High Activity of Subtilisin Carlsberg in Ionic Liquids by Chemical Modification with Comb-Shaped Poly(ethylene glycol)
NAKASHIMA Kazunori; MARUYAMA Tatsuo; KAMIYA Noriho; GOTO Masahiro, 日本生物工学会大会講演要旨集, 17, 100, 100, 2005
日本生物工学会, Japanese

イオン性液体中における均一酵素反応場の構築
中島 一紀; 丸山 達生; 神谷 典穂; 後藤 雅宏, 化学工学会 研究発表講演要旨集, 2005, 286, 286, 2005
公益社団法人 化学工学会, Japanese

エコバイオリファイナリー―脱石油社会へ移行するための環境ものづくり戦略―
シーエムシー出版, 2010

酵素利用技術大系
エヌ・ティー・エス, 2010

エコバイオリファイナリー―脱石油社会へ移行するための環境ものづくり戦略―
シーエムシー出版, 2010

酵素利用技術大系
エヌ・ティー・エス, 2010

資源生物工学, 2024年, 修士課程, 工学院

資源生物工学, 2024年, 修士課程, 工学院

資源生物工学特論, 2024年, 修士課程, 工学院

資源生物工学特論, 2024年, 修士課程, 工学院

資源生物工学特論, 2024年, 博士後期課程, 工学院

資源生物工学特論, 2024年, 博士後期課程, 工学院

資源循環システム実験Ⅰ, 2024年, 学士課程, 工学部

資源循環システム実験Ⅲ, 2024年, 学士課程, 工学部

化学Ⅱ, 2024年, 学士課程, 全学教育

微生物工学, 2024年, 学士課程, 工学部

Development of Hydraulic Shielding Properties ofPorous Rocks Using the Enzyme-Induced Calcite Precipitation Method
Grants-in-Aid for Scientific Research
01 Apr. 2024 - 31 Mar. 2027
佐藤 晃; 中島 一紀; 才ノ木 敦士; 伊藤 紘晃
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Kumamoto University, 24K01421

Selective and intelligent aggregation system using biological functions
Grants-in-Aid for Scientific Research
01 Apr. 2024 - 31 Mar. 2027
中島 一紀; 廣吉 直樹; 五十嵐 健輔; 高野 力
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 24K03104

天然および人工の有機・無機接着界面から発想する新たなバイオベース固化技術の創出
科学研究費助成事業
01 Apr. 2021 - 31 Mar. 2024
中島 一紀; 川崎 了; 五十嵐 健輔
日本学術振興会, 基盤研究(B), 北海道大学, 21H03627

デザイン可能なバイオベースの貴金属吸着剤の創製
科学研究費助成事業
30 Jul. 2020 - 31 Mar. 2023
中島 一紀
本研究では，バイオマスとタンパク質を組み合わせることで都市鉱山からの貴金属の回収が可能なバイオベースの金属イオン吸着剤を開発する。
昨年度の研究において，金結合タンパク質（AuBP）と多糖結合モジュール（CBM）を融合した融合タンパク質AuBP-CBMを作製し，そのAu3+吸着機能が確認された。そこで本年度は，様々な融合タンパク質のラインナップを作製し，そのセルロース吸着機能および金属吸着機能を調査した。融合タンパク質の作製の過程において，遺伝子構築およびタンパク質発現に成功したものを用いてその後の実験を行った。AuBP-CBMをベースタンパク質として，CBMのN末端およびC末端の両方にAuBPを配置したAuBP両端型，AuBP-CBM を2つ連結したCBMタンデム型を作製した。これら3つの融合タンパク質のセルロース吸着性および金属結合性はそれぞれ異なったが，最終的な効率を示すセルロースあたりのAu3+回収率はAuBP両端型が最も高かった。また，X線光電子分光法（XPS）により，セルロース上に吸着したAuがAu(0)であることを確認した。AuBP両端型を固定化したセルロースは，白金族および遷移金属（Au, Pt, Pd, Cu, Co, Ni, Zn）を含む溶液中からAuを高選択的に回収することが可能であり，セルロース上のAuはチオ尿素を用いて89%脱着することができた。この際，AuはAu+に酸化され，チオ尿素と錯体を形成したと考えられる。
また，ろ紙以外のセルロース材料として，高機能性材料として注目を集めているセルロースナノファイバーの利用を検討した。タンパク質の吸着性について調査したところ，ろ紙よりも3倍以上の高い吸着性を示すことが明らかとなった。
日本学術振興会, 挑戦的研究(萌芽), 北海道大学, 20K21853

Consolidated bioremediation of heavy metal contamination using biocementation and biomass
Grants-in-Aid for Scientific Research
01 Apr. 2018 - 31 Mar. 2021
Kazunori Nakashima
The objective of this study is to develop a system that can immobilize soil contaminated with high concentration of heavy metals using bio-cement technique, and completely remove a trace amount of heavy-metal ions in water using heavy-metal-binding biomass. Precipitation of CaCO3 using urease-producing bacteria was successfully applied for solidification and immobilization of heavy metals in a model and actual contaminated soil samples. Furthermore, bio-cement combined with organic polymers was developed to increase the strength of biocemented sample. Novel heavy metal adsorbents based on protein and natural polymers were developed, and a continuous adsorption system was constructed.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 18H03395

Development of microbial cell able to spontaneously form hybrid with inorganic materials and application to microbial fuel cell
Grants-in-Aid for Scientific Research
01 Apr. 2015 - 31 Mar. 2017
Nakashima Kazunori
Silicatein is an enzyme which catalyzes silica polycondensation in marine sponge. The enzyme can produce silica from siliceous substrate such as tetraethoxysilane under mild condition, i.e. room temperature and neutral pH. In the present work, we examined the aggregation of silicatein expressed in E. coli. Silicatein gene optimized for the expression in E. coli was successfully expressed using cold shock vector. Although most of the protein was expressed in insoluble fraction, silicatein could be solubilized by refolding. The refolded silicatein was used for the self-assembly test under several conditions. Silicatein self-assembled to form aggregation in buffer solution while the aggregation was suppressed in the presence of chaotropic reagent, urea. Cell surface display system has been developed using InaK from Pseudomonas syringae and OmpA from E. coli. Furthermore, microbial fuel cell system is also constructed.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, 15K18273

A novel reaction and separation technology for simultaneous production of bioactive compounds and biofuels from oil biomass
Grants-in-Aid for Scientific Research
01 Apr. 2012 - 31 Mar. 2015
YONEMOTO Toshikuni; KITAKAWA Naomi; NAKASHIMA Kazunori
Oil biomass contains triglyceride, its hydrolysate (free fatty acid, FFA) and lipid-soluble bioactive compounds such as vitamin E (tocopherol and tocotrienol) and phytosterol. However, no industrial technology to efficiently recover bioactive compounds with lower thermal stability has been established. We have proposed a novel reaction and separation technology at 50 ℃ and atmospheric pressure using ion-exchange resins as catalysts and adsorbents. The technology enables the complete conversions of both triglyceride and FFA to biodiesel and selective recovery of vitamin E from oils. Especially, tocotrienol has succeeded to be recovered at the highest ratio, more than 60 %. The solubility of phytosterol in biodiesel becomes lower than that in oils, so that phytosterol has been crystallized at room temperature and easily recovered. Therefore, this novel technology to simultaneously produce vitamin E, phyrosterol and biodiesel from biomass gives profitability to the enterprise.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Tohoku University, 24246127

Research for tocotrienol recovery and biodiesel production from waste rice bran oil
Grants-in-Aid for Scientific Research
2009 - 2011
YONEMOTO Toshikuni; SHIBASAKI Kitakawa naomi; KUBO Masaki; NAMASHIMA Kazunori
The purpose of this research is to construct a novel reaction and separation process for producing bioactive compounds, tocotrienol (vitamin E), and renewable energy, high quality biodiesel, from waste oils discharged in the vegetable oil refining process. The process consists of the esterification of free fatty acid using cation-exchange resin catalyst, the adsorption of tocotrienol on the anion-exchange resin, the desorption of tocotrienol from the resin and the transesterification of triglyceride using anion-exchange resin catalyst. The optimal conditions for continuous operation in each step were investigated and elucidated.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Tohoku University, 21360373