Researcher basic information

• 博士(環境学), 東京大学

• https://researchmap.jp/maoshiki?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201501048419737354

• 201501048419737354

• 下水処理
• 活性汚泥
• biofilm
• biological nitrogen removal
• nitrification - denitrification
• 嫌気好気法
• partial nitrification
• anaerobic ammonium oxidation
• biological phosphorus removal
• anaerobic digestion
• methane fermentation
• 嫌気性原生動物
• 尿素分解
• DNA/RNA解析
• 定量PCR(qPCR)
• 微少流路デバイス
• 16S RNA遺伝子アンプリコン解析
• メタゲノム解析
• RNASeq解析
• 遺伝子等温増幅(RPA)
• 薬剤耐性遺伝子
• 遺伝子組換え実験
• antibiotic resistance plasmids
• 遺伝子クローニング
• bioplastic
• PHA
• 微生物培養
• 嫌気性培養
• 顕微鏡イメージング
• 安定同位体比測定
• 安定同位体トレーサー試験
• 質量分析
• 嫌気性アンモニウム酸化(anammox)菌
• アンモニア酸化細菌(AOB)
• アンモニア酸化古細菌(AOA)
• 亜酸化窒素(N2O)
• ヒドロキシルアミン(NH2OH)/ヒドラジン(N2H4)
• thiocyanate
• 鉄酸化硝酸還元

• Social Infrastructure (Civil Engineering, Architecture, Disaster Prevention), Environmental systems for civil engineering, 生物学的窒素除去、硝化・脱窒、嫌気性アンモニウム酸化(anammox)、薬剤耐性菌、生物電気化学的プロセス
• Environmental Science/Agriculture Science, Environmental impact assessment, 窒素循環、窒素安定同位体、薬剤耐性遺伝子、薬剤耐性菌
• Environmental Science/Agriculture Science, Environmental load reduction and remediation, 排水処理、生物処理、環境負荷低減
• Life Science, Applied microbiology, 窒素代謝微生物、嫌気性アンモニウム酸化(anammox)菌
• Life Science, Aquatic life science, 窒素循環、窒素代謝

• Bachelor's degree program, School of Engineering
• Master's degree program, Graduate School of Engineering
• Doctoral (PhD) degree program, Graduate School of Engineering

Research activity information

• Aug. 2025, クリタ水・環境科学財団, クリタ水・環境科学研究優秀賞
• Mar. 2024, 日本水環境学会, ライオン賞
  活性汚泥に生息する従属栄養硝化-好気性脱窒細菌の特定
• Mar. 2023, ISME Journal, ISME Journal Outstanding Editorial Board Member Award 2022
• Mar. 2023, 日本水環境学会, ライオン賞
  石崎翔大;石塚祐介;樋口宏介;押木守;中屋佑紀;佐藤久
• Mar. 2023, 日本水環境学会, ライオン賞
  小原紀子;樋口宏介;北島正章;岡部聡;押木守
• Mar. 2022, 日本水環境学会, クリタ賞
  樋口宏介,北島正章,岡部 聡,押木 守
• 2021, 長瀬科学技術振興財団, 長瀬研究振興賞
• 2019, 日本微生物生態学会, Microbes & Environments 2018 最優秀論文賞
  押木 守
• 2018, インテリジェント・コスモス, インテリジェント・コスモス奨励賞
  押木 守
• 2018, 第28回日本MRS年次大会, 奨励賞
  押木 守
• 2017, イムラ・ジャパン, 第1回イムラ・ジャパン賞
  押木 守
• 2017, 建設工学研究振興会, 建設工学奨励賞
  押木 守
• 2011, 日本微生物生態学会, 第27回日本微生物生態学会 優秀ポスター賞
  押木 守
• 2011, アナモックスシンポジウム, 第一回国際アナモックスシンポジウム 最優秀講演賞
  押木 守
• 2011, 国際硝化学会, 第2回国際硝化学会・第16回ヨーロッパ窒素循環会議 発表賞
  押木 守
• 2008, 日本水環境学会, 博士研究奨励賞 (オルガノ賞)
  押木 守
• 2002, 全国高専土木学会, 近藤賞
  押木 守

• Horizontal and vertical gene transfer shape the plasmid host range in surface-associated microbial systems.
  Kohei Takahashi; Kiko Ohara; Kosuke Higuchi; Takuya Ohmura; Satoshi Okabe; David R Johnson; Mamoru Oshiki
  iScience, 29, 4, 115299, 115299, 17 Apr. 2026, [International Magazine]
  English, Scientific journal, Broad-host-range plasmids drive the spread of antibiotic resistance, particularly in surface-associated microbial systems prevalent in natural and host-associated environments. Predicting their realized host range is challenging because both transconjugant proliferation (vertical gene transfer, VGT) and conjugation (horizontal gene transfer, HGT) contribute to transconjugant diversity. Here, we hypothesized that the realized host range is determined by the interplay between VGT and HGT. We experimentally tested this hypothesis by analyzing transconjugant diversity under conditions that differ in their ability to support bacterial growth. Fast-growth conditions increased transconjugant abundance but reduced diversity, whereas slow-growth conditions supported fewer but more diverse transconjugants. We complemented these experiments with individual-based simulations that explicitly incorporated both VGT and HGT. Our results demonstrate that the realized host range is jointly governed by initial HGT events and subsequent VGT-driven expansion, highlighting the importance of integrating transfer and post-transfer dynamics when predicting plasmid-mediated antibiotic resistance spread.
• Oxygen scavenging enables microoxic survival of the marine anammox bacterium Scalindua sp.
  Satoshi Okabe; Keishi Nukada; Keitaro Horiguchi; Mamoru Oshiki
  The ISME journal, 02 Apr. 2026, [International Magazine]
  English, Scientific journal, Marine anammox bacteria of the genus Scalindua play a pivotal role in nitrogen loss within oceanic oxygen minimum zones (OMZs), yet their physiological resilience to oxygen exposure remains poorly characterized. Here, we show that Scalindua sp. possesses an intrinsic capacity to reduce ambient oxygen under microoxic conditions (headspace O₂ ≈ 3%), exhibiting specific oxygen reduction rates (SORRs) of ~10 nmol-O₂ min-1 mg- protein-1. Anammox activity, evidenced by 29N₂ production, resumed once dissolved oxygen (DO) declined below ~5 μM, defining the DO threshold. This oxygen-scavenging ability is likely mediated by direct oxygen reduction to water via cbb₃-type cytochrome c oxidase (CcO) and A-type flavodiiron proteins (Fdps), with minimal reactive oxygen species (ROS) generation. CcO activity increased rapidly and markedly upon nitrite addition, consistent with the immediate onset of oxygen consumption and the transcriptional induction of CcO subunit genes. Nitrite was essential for sustaining oxygen consumption, and the rapid CcO activation suggests that nitrite stimulates CcO function through an unresolved regulatory mechanism. Nitrite oxidation also supplies supplementary electrons for oxygen reduction independently of core anammox metabolism, underscoring a flexible substrate-driven detoxification mechanism that enables Scalindua sp. to cope with transient microoxia. Together, these results show that Scalindua sp. employs a multi-layered defense -moderate oxygen-reduction capacity coupled with efficient ROS detoxification- conferring high and reversible oxygen tolerance. Although laboratory conditions cannot fully replicate natural OMZ complexity, our findings indicate that this physiological flexibility reflects the potential ecological resilience of Scalindua sp. in dynamic ocean environments.
• In situ biogas upgrading performance, microbial community structures, and metabolic potentials during biomethanation in a thermophilic anaerobic membrane bioreactor treating sewage sludge
  Akira Hafuka; Mamoru Oshiki; Sota Fujino; Kohei Takahashi; Satoshi Okabe; Ryusei Ito; Shiho Mizuno; Kazuyuki Oshita; Katsuki Kimura
  ACS ES&T Water, (in press), Oct. 2025, [Peer-reviewed]
• Residual ammonia shapes nitrite-oxidizing activity and microbial spatial dynamics in gel-immobilized anammox biomass under mainstream conditions
  Hyungmin Choi; Jihun Park; Danbee Kim; Mamoru Oshiki; Satoshi Okabe; Changsoo Lee
  Chemical Engineering Journal, 519, 165408, 165408, Elsevier BV, Sep. 2025
  Scientific journal
• Nitrifying communities in biological nitrogen removal processes at tropical municipal wastewater treatment plants
  Liang Feng; Jia Xing Loi; Joana Séneca; Petra Pjevac; Faidzul Hakim Adnan; Gek Cheng Ngoh; Bee Chin Khor; Alijah Mohd Aris; Mamoru Oshiki; Holger Daims; Adeline Seak May Chua
  Microbes & Environments, 40, 3, ME25036, Sep. 2025, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Nitrifying communities in activated sludge play a crucial role in biological nitrogen removal processes in municipal wastewater treatment plants. While extensive research has been conducted in temperate regions, limited information is available on nitrifiers in tropical regions. The present study investigated all currently known nitrifying communities in two full-scale municipal wastewater treatment plants in Malaysia operated under low-dissolved oxygen (DO) (0.2-0.7‍ ‍mg‍ ‍DO‍ ‍L-1) or high-DO (2.0-5.5‍ ‍mg‍ ‍DO‍ ‍L-1) conditions at 30°C. The core nitrifiers in the municipal wastewater treatment plants were Nitrosomonas (ammonia-oxidizing bacteria, AOB), Nitrospira (nitrite-oxidizing or complete ammonia-oxidizing, comammox, bacteria), and ammonia-oxidizing archaea (AOA) as identified by a 16S rRNA gene amplicon sequencing ana-lysis and corroborated by 16S rRNA-targeted fluorescence in situ hybridization. A quantitative polymerase chain reaction (qPCR) targeting ammonia monooxygenase subunit A (amoA) genes revealed stable populations of comammox Nitrospira and AOB in both wastewater treatment plants. AOA were detected in only one of the plants and their population sizes fluctuated, with higher temporary abundance under high-DO conditions. These results provide important insights into the composition and dynamics of nitrifying communities in tropical municipal wastewater treatment plants.
• Multivariate regression modeling in integrative analysis via sparse regularization
  Shuichi Kawano; Toshikazu Fukushima; Junichi Nakagawa; Mamoru Oshiki
  Japanese Journal of Statistics and Data Science, Springer Science and Business Media LLC, 02 Aug. 2025, [Peer-reviewed], [Last author]
  Scientific journal
• Interspecific competition and adaptation of anammox bacteria at different salinities: Experimental validation of the Monod growth model with salinity inhibition
  Satoshi Okabe; Akimichi Kamizono; Seiya Kawasaki; Kanae Kobayashi; Mamoru Oshiki
  Water Research, 271, 122883, Mar. 2025, [Peer-reviewed], [Last author]
  Scientific journal
• Oxygen isotope fractionation during anaerobic ammonium oxidation by the marine representative Candidatus Scalindua sp.
  Kanae Kobayashi; Kazuya Nishina; Keitaro Fukushima; Yuji Onishi; Akiko Makabe; Mamoru Oshiki; Keisuke Koba; Satoshi Okabe
  The ISME journal, 19, 1, wraf115, 02 Jan. 2025, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Analysing the nitrogen (15ε) and oxygen (18ε) isotope effects of anaerobic ammonium oxidation (anammox) is essential for accurately assessing its potential contribution to fixed-N losses in the ocean, yet the 18ε of anammox remains unexplored. Here, we determined the previously unexplored 18ε of anammox using a highly enriched culture of the marine anammox species "Ca. Scalindua sp". Because Scalindua significantly accelerated oxygen isotope exchange between NO2- and H2O, we introduced a new rate constant for anammox-mediated oxygen isotope exchange (keq, AMX = 8.44 ~ 13.56 × 10-2 h-1), which is substantially faster than abiotic oxygen isotope exchange (keq, abio = 1.13 × 10-2 h-1), into a numerical model to estimate the 18ε during anammox. Based on our experimental results, we successfully determined the 18ε associated with: (1) conversion of NO2- to N2 (18εNO2- → N2 = 10.6 ~ 16.1‰), (2) NO2- oxidation to NO3- (18εNO2- → NO3- = -2.9 ~ -11.0‰, inverse fractionation), (3) incorporation of oxygen from water during NO2- oxidation to NO3- (18εH2O = 16.4 ~ 19.2‰). Our study underscores the possibility that unique anammox oxygen isotope signals may be masked due to substantial anammox-mediated oxygen isotope exchange between NO2- and H2O. Therefore, careful consideration is required when utilizing δ18ONO3- and δ18ONO2- as geochemical markers to assess the potential contribution of anammox to fixed-N losses in the ocean.
• Microbial community structure of mesophilic and low-temperature partial nitrification-anammox reactors: distribution and functional roles of the core microbiome
  Mamoru Oshiki; Kohei Takahashi; Seiya Kawasaki; Hyungmin Choi; Jihye Park; Kwiyong Kim; Hyokwan Bae; Satoshi Okabe; Changsoo Lee
  Microbes and environments, 40, 2, ME25001, 2025, [Peer-reviewed], [Lead author, Corresponding author], [Domestic magazines]
  English, Scientific journal, Microbial community structures in mesophilic and low-temperature anammox and partial nitrification-anammox reactors were exami-ned by a 16S rRNA-gene amplicon sequencing ana-lysis. The anammox bacterium, Jettenia sp., was dominant, and nitrifying bacteria, including Nitrosomonas sp. (aerobic ammonia-oxidizing bacterium) and Nitrospira sp., (nitrite-oxidizing bacterium) coexisted in the bioreactors. Core coexisting bacteria, such as Sulfurisoma sp. and Zeimonas sp., showed oxygen-scavenging and NO3- reduction potentials. Sulfurisoma-related bacteria are distributed across wastewater treatment plants worldwide, particularly in denitrification systems. These results underscore the ecological and functional importance of microbial consortia in enhancing nitrogen removal efficiency.
• Thermophilic dark fermentation for hydrogen and volatile fatty acids production from breadcrumbs
  Oshiki, M.; Yamaguchi, G.; Takahashi, K.; Okabe, S.; Kawano, S.; Nakagawa, J.; Fukushima, T.
  Chemical Engineering Journal, 501, 157633, Dec. 2024, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• アンモニア酸化細菌のバイオフィルム形成を利用した高密度培養
  押木 守
  バイオサイエンスとインダストリー (B & I), 82, 390, 391, Jul. 2024, [Peer-reviewed], [Invited], [Lead author, Corresponding author]
• Salinity tolerance and osmoadaptation strategies in four genera of anammox bacteria: Brocadia, Jettenia, Kuenenia, and Scalindua.
  Satoshi Okabe; Akimichi Kamizono; Lei Zhang; Seiya Kawasaki; Kanae Kobayashi; Mamoru Oshiki
  Environmental Science & Technology, 58, 12, 5357–5371, American Chemical Society (ACS), 16 Mar. 2024, [Peer-reviewed], [Last author]
  Scientific journal
• Collaborative metabolisms of urea and cyanate degradation in marine anammox bacterial culture
  Mamoru Oshiki; Emi Morimoto; Kanae Kobayashi; Hisashi Satoh; Satoshi Okabe
  ISME Communications, 4, 1, ycad007, Oxford University Press (OUP), 01 Jan. 2024, [Peer-reviewed], [Lead author]
  Scientific journal, Abstract
  
  Anammox process greatly contributes to nitrogen loss occurring in oceanic oxygen minimum zones (OMZs), where the availability of NH4+ is scarce as compared with NO2−. Remineralization of organic nitrogen compounds including urea and cyanate (OCN−) into NH4+ has been believed as an NH4+ source of the anammox process in oxygen minimum zones. However, urea- or OCN−- dependent anammox has not been well examined due to the lack of marine anammox bacterial culture. In the present study, urea and OCN− degradation in a marine anammox bacterial consortium were investigated based on 15N-tracer experiments and metagenomic analysis. Although a marine anammox bacterium, Candidatus Scalindua sp., itself was incapable of urea and OCN− degradation, urea was anoxically decomposed to NH4+ by the coexisting ureolytic bacteria (Rhizobiaceae, Nitrosomonadaceae, and/or Thalassopiraceae bacteria), whereas OCN− was abiotically degraded to NH4+. The produced NH4+ was subsequently utilized in the anammox process. The activity of the urea degradation increased under microaerobic condition (ca. 32–42 μM dissolved O2, DO), and the contribution of the anammox process to the total nitrogen loss also increased up to 33.3% at 32 μM DO. Urea-dependent anammox activities were further examined in a fluid thioglycolate media with a vertical gradient of O2 concentration, and the active collaborative metabolism of the urea degradation and anammox was detected at the lower oxycline (21 μM DO).
• Denitrification in low oxic environments increases the accumulation of nitrogen oxide intermediates and modulates the evolutionary potential of microbial populations.
  Kohei Takahashi; Mamoru Oshiki; Chujin Ruan; Kana Morinaga; Masanori Toyofuku; Nobuhiko Nomura; David R Johnson
  Environmental microbiology reports, 16, 1, e13221, 30 Nov. 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Denitrification in oxic environments occurs when a microorganism uses nitrogen oxides as terminal electron acceptors even though oxygen is available. While this phenomenon is well-established, its consequences on ecological and evolutionary processes remain poorly understood. We hypothesize here that denitrification in oxic environments can modify the accumulation profiles of nitrogen oxide intermediates with cascading effects on the evolutionary potentials of denitrifying microorganisms. To test this, we performed laboratory experiments with Paracoccus denitrificans and complemented them with individual-based computational modelling. We found that denitrification in low oxic environments significantly increases the accumulation of nitrite and nitric oxide. We further found that the increased accumulation of these intermediates has a negative effect on growth at low pH. Finally, we found that the increased negative effect at low pH increases the number of individuals that contribute to surface-associated growth. This increases the amount of genetic diversity that is preserved from the initial population, thus increasing the number of genetic targets for natural selection to act upon and resulting in higher evolutionary potentials. Together, our data highlight that denitrification in low oxic environments can affect the ecological processes and evolutionary potentials of denitrifying microorganisms by modifying the accumulation of nitrogen oxide intermediates.
• 異なる溶存酸素条件が複合微生物系における好気性脱窒細菌群に与える影響
  上村 光輝; 川上 周司; 押木 守; 青木 仁孝; 土田 勝範; 渡利 高大; 荒木 信夫
  土木学会論文集, 79, 25, 23-25034, Nov. 2023, [Peer-reviewed]
• Growth of the Nitrosomonas europaea cells in the biofilm and planktonic growth mode: Responses of extracellular polymeric substances production and transcriptome
  Mamoru Oshiki; Takahiro Saito; Yuki Nakaya; Hisashi Satoh; Satoshi Okabe
  Journal of Bioscience and Bioengineering, 136, 6, 430-437, Elsevier BV, Nov. 2023, [Peer-reviewed], [Lead author, Corresponding author]
  Scientific journal
• Oxygen tolerance and detoxification mechanisms of highly enriched planktonic anaerobic ammonium-oxidizing (anammox) bacteria
  Satoshi Okabe; Shaoyu Ye; Xi Lan; Keishi Nukada; Haozhe Zhang; Kanae Kobayashi; Mamoru Oshiki
  ISME Communications, 3, 1, 45 (2023), Springer Science and Business Media LLC, 03 May 2023, [Peer-reviewed], [Last author]
  Scientific journal, Abstract
  
  Oxygen is a key regulatory factor of anaerobic ammonium oxidation (anammox). Although the inhibitory effect of oxygen is evident, a wide range of oxygen sensitivities of anammox bacteria have been reported so far, which makes it difficult to model the marine nitrogen loss and design anammox-based technologies. Here, oxygen tolerance and detoxification mechanisms of four genera of anammox bacteria; one marine species (“Ca. Scalindua sp.”) and four freshwater anammox species (“Ca. Brocadia sinica”, “Ca. Brocadia sapporoensis”, “Ca. Jettenia caeni”, and “Ca. Kuenenia stuttgartiensis”) were determined and then related to the activities of anti-oxidative enzymes. Highly enriched planktonic anammox cells were exposed to various levels of oxygen, and oxygen inhibition kinetics (50% inhibitory concentration (IC₅₀) and upper O₂ limits (DO_max) of anammox activity) were quantitatively determined. A marine anammox species, “Ca. Scalindua sp.”, exhibited much higher oxygen tolerance capability (IC₅₀ = 18.0 µM and DO_max = 51.6 µM) than freshwater species (IC₅₀ = 2.7–4.2 µM and DO_max = 10.9–26.6 µM). The upper DO limit of “Ca. Scalindua sp.” was much higher than the values reported so far (~20 µM). Furthermore, the oxygen inhibition was reversible even after exposed to ambient air for 12–24 h. The comparative genome analysis confirmed that all anammox species commonly possess the genes considered to function for reduction of O₂, superoxide anion (O₂^•-), and H₂O₂. However, the superoxide reductase (Sor)-peroxidase dependent detoxification system alone may not be sufficient for cell survival under microaerobic conditions. Despite the fact that anaerobes normally possess no or little superoxide dismutase (Sod) or catalase (Cat), only Scalindua exhibited high Sod activity of 22.6 ± 1.9 U/mg-protein with moderate Cat activity of 1.6 ± 0.7 U/mg-protein, which was consistent with the genome sequence analysis. This Sod-Cat dependent detoxification system could be responsible for the higher O₂ tolerance of Scalindua than other freshwater anammox species lacking the Sod activity.
• 安定同位体を用いた窒素循環に関わる微生物代謝活性評価
  押木守
  バイオサイエンスとインダストリー, 81, 3, 200, 204, May 2023, [Peer-reviewed], [Invited], [Lead author, Corresponding author]
• Growth of nitrite-oxidizing Nitrospira and ammonia-oxidizing Nitrosomonas in marine recirculating trickling biofilter reactors.
  Mamoru Oshiki; Hirotoshi Netsu; Kyohei Kuroda; Takashi Narihiro; Naoki Fujii; Tomonori Kindaichi; Yoshiyuki Suzuki; Takahiro Watari; Masashi Hatamoto; Takashi Yamaguchi; Nobuo Araki; Satoshi Okabe
  Environmental microbiology, 24, 8, 3735, 3750, 07 Jun. 2022, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, Aerobic ammonia and nitrite oxidation reactions are fundamental biogeochemical reactions contributing to the global nitrogen cycle. Although aerobic nitrite oxidation yields 4.8-folds less Gibbs free energy (∆Gr ) than aerobic ammonia oxidation in the NH4 + -feeding marine recirculating trickling biofilter reactors operated in the present study, nitrite-oxidizing and not ammonia-oxidizing Nitrospira (sublineage IV) outnumbered ammonia-oxidizing Nitrosomonas (relative abundance; 53.8% and 7.59% respectively). CO2 assimilation efficiencies during ammonia or nitrite oxidation were 0.077 μmol-14 CO2 /μmol-NH3 and 0.053-0.054 μmol-14 CO2 /μmol-NO2 - respectively, and the difference between ammonia and nitrite oxidation was much smaller than the difference of ∆Gr . Free-energy efficiency of nitrite oxidation was higher than ammonia oxidation (31%-32% and 13% respectively), and high CO2 assimilation and free-energy efficiencies were a determinant for the dominance of Nitrospira over Nitrosomonas. Washout of Nitrospira and Nitrosomonas from the trickling biofilter reactors was also examined by quantitative PCR assay. Normalized copy numbers of Nitrosomonas amoA were 1.5- to 1.7-folds greater than Nitrospira nxrB and 16S rRNA gene in the reactor effluents. Nitrosomonas was more susceptible for washout than Nitrospira in the trickling biofilter reactors, which was another determinant for the dominance of Nitrospira in the trickling biofilter reactors.
• NH₂OH disproportionation mediated by anaerobic ammonium-oxidizing (anammox) bacteria
  Mamoru Oshiki; Lin Gao; Lei Zhang; Satoshi Okabe
  Microbes Environ., 37, 2, ME21092, n/a, Japanese Society of Microbial Ecology, Apr. 2022, [Peer-reviewed], [Lead author, Corresponding author]
  Scientific journal
• N₂O reduction by Gemmatimonas aurantiaca and potential involvement of Gemmatimonadetes bacteria in N₂O reduction in agricultural soils
  Mamoru Oshiki; Yuka Toyama; Toshikazu Suenaga; Akihiko Terada; Yasuhiro Kasahara; Takashi Yamaguchi; Nobuo Araki
  Microbes Environ., 37, 2, ME21090, n/a, Japanese Society of Microbial Ecology, Apr. 2022, [Peer-reviewed], [Lead author, Corresponding author]
  Scientific journal
• Determination of ¹⁵ N/¹⁴N of ammonium, nitrite, nitrate, hydroxylamine, and hydrazine using colorimetric reagents and matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS)
  Mamoru Oshiki; Komei Nagai; Satoshi Ishii; Yoshiyuki Suzuki; Nobuo Saito; Takashi Yamaguchi; Nobuo Araki; Satoshi Okabe
  Appl. Environ. Microbiol., 88, 9, e0241621, American Society for Microbiology, 14 Mar. 2022, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, ¹⁵ N/ ¹⁴ N analysis for aqueous N species is a powerful tool for obtaining insights into the global N cycle, but the procedure is cumbersome and laborious. The combined use of colorimetric reagents and MALDI-TOF MS, designated color MALDI-TOF MS, enabled us to determine the ¹⁵ N atom% of common aqueous N species without laborious sample preparation and chromatographic separation steps; for instance, the ¹⁵ N atom% of NO ₂⁻ can be determined from >1,000 liquid samples daily at <$1 (U.S.) per 384 samples for routine analysis.
• Full-scale application of a down-flow hanging sponge reactor combined with a primary sedimentation basin for domestic sewage treatment
  Tsutomu Okubo; Tadashi Tagawa; Masanobu Takahashi; Akinori Iguchi; Mamoru Oshiki; Nobuo Araki; Kengo Kubota; Ahmed Tawfik; Shigeki Uemura; Hideki Harada
  Bioprocess Biosyst. Eng., 45, 4, 701, 709, Springer Science and Business Media LLC, 31 Jan. 2022, [Peer-reviewed]
  Scientific journal
• Metagenomic analysis of five phylogenetically distant anammox bacterial enrichment cultures
  Mamoru Oshiki; Yoshihiro Takaki; Miho Hirai; Takuro Nunoura; Atsushi Kamigaito; Satoshi Okabe
  Microbes and environments, 37, 3, ME22017, 2022, [Peer-reviewed], [Lead author, Corresponding author], [Domestic magazines]
  English, Scientific journal, Anaerobic ammonium-oxidizing (anammox) bacteria are slow-growing and fastidious bacteria, and limited numbers of enrichment cultures have been established. A metagenomic ana-lysis of our 5 established anammox bacterial enrichment cultures was performed in the present study. Fourteen high-quality metagenome-assembled genomes (MAGs) were obtained, including those of 5 anammox Planctomycetota (Candidatus Brocadia, Ca. Kuenenia, Ca. Jettenia, and Ca. Scalindua), 4 Bacteroidota, and 3 Chloroflexota. Based on the gene sets of metabolic pathways involved in the degradation of polymeric substances found in Chloroflexota and Bacteroidota MAGs, they are expected to be scavengers of extracellular polymeric substances and cell debris.
• Endpoint recombinase polymerase amplification (RPA) assay for enumeration of thiocyanate-degrading bacteria
  Mamoru Oshiki; Toshikazu Fukushima; Shuichi Kawano; Junichi Nakagawa
  Microbes Environ., 37, 1, ME21073, n/a, Japanese Society of Microbial Ecology, 2022, [Peer-reviewed], [Lead author, Corresponding author]
  Scientific journal
• 海洋性Anammox細菌の脱アンモニウム能に及ぼすpHの影響：至適pH制御による脱アンモニウム能の回復
  田中孝国; 岡悟史; 押木守; 金田一智規; 新田見匡
  工業用水, 669, 51, 55, Nov. 2021, [Peer-reviewed]
• 異なるpH条件下における 海洋性Anammox細菌の脱アンモニウム能
  田中孝国; 岡悟史; 田中昭雄; 押木守; 金田一智規; 新田見匡
  工業用水, 667, 76, 82, Jul. 2021, [Peer-reviewed]
• Glycogen metabolism of the anammox bacterium "Candidatus Brocadia sinica".
  Satoshi Okabe; Amrini Amalia Shafdar; Kanae Kobayashi; Lei Zhang; Mamoru Oshiki
  ISME J., 15, 5, 1287, 1301, May 2021, [Peer-reviewed], [Last author], [International Magazine]
  English, Scientific journal, Presence of glycogen granules in anaerobic ammonium-oxidizing (anammox) bacteria has been reported so far. However, very little is known about their glycogen metabolism and the exact roles. Here, we studied the glycogen metabolism in "Ca. Brocadia sinica" growing in continuous retentostat cultures with bicarbonate as a carbon source. The effect of the culture growth phase was investigated. During the growing phase, intracellular glycogen content increased up to 32.6 mg-glucose (g-biomass dry wt)-1 while the specific growth rate and ATP/ADP ratio decreased. The accumulated glycogen begun to decrease at the onset of entering the near-zero growth phase and was consumed rapidly when substrates were depleted. This clearly indicates that glycogen was synthesized and utilized as an energy storage. The proteomic analysis revealed that "Ca. B. sinica" synthesized glycogen via three known glycogen biosynthesis pathways and simultaneously degraded during the progress of active anammox, implying that glycogen is being continuously recycled. When cells were starved, a part of stored glycogen was converted to trehalose, a potential stress protectant. This suggests that glycogen serves at least as a primary carbon source of trehalose synthesis for survival. This study provides the first physiological evidence of glycogen metabolism in anammox bacteria and its significance in survival under natural substrate-limited habitat.
• Complete genome sequence of Thiohalobacter sp. strain COW1, isolated from activated sludge treating coke oven wastewater
  Kentaro Miyazaki; Hikaru Suenaga; Mamoru Oshiki; Shuichi Kawano; Toshikazu Fukushima
  Microbiol. Resour. Announc., 10, 7, e00013-21, American Society for Microbiology, 18 Feb. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal,
  A thiocyanate-degrading bacterium, Thiohalobacter sp. strain COW1, was isolated from activated sludge treating coke oven wastewater, and the complete genome sequence was determined. COW1 contained a single circular chromosome (3.23 Mb; G+C content, 63.4%) in which 2,788 protein-coding genes, 39 tRNA genes, and 3 rRNA genes were identified.
• 海洋に流出した窒素除去を目的とした海洋性anammox細菌の研究
  田中考国; 押木守; 金田一智規; 新田見匡
  分離技術, 51, 1, 32, 41, Feb. 2021, [Peer-reviewed]
• Draft genome sequence of Cytophagales sp. strain WSM2-2, isolated from garden soil
  Masataka Aoki; Mamoru Oshiki; Mizuki Iwagaki; Masashi Hatamoto; Takashi Yamaguchi; Nobuo Araki
  Microbiol. Resour. Announc., 10, 2, e01238-20, American Society for Microbiology, 14 Jan. 2021, [Peer-reviewed]
  Scientific journal
• Draft genome sequence of Enterobacter sp. AS-1, a potential eurytrophic recombination host
  Yuki Iwasaki; Yuya Itoiri; Sota Ihara; Hironaga Akita; Mamoru Oshiki; Zen-ichiro Kimura
  J. Genomics, 9, 6, 9, Ivyspring International Publisher, 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Strain AS-1 was isolated from laboratory-scale activated sludge collected in Japan. This strain not only grows on rich medium, including R2A medium, but also forms colonies on medium lacking organic matter other than agar (water agar), indicating it could be used as a eurytrophic recombinant host in material production processes. Here, we present a draft genome sequence of Enterobacter sp. AS-1, which consists of a total of 24 contigs containing 5,207,146 bp, with a GC content of 55.64%, and comprising 4,921 predicted coding sequences. Based on 16S rRNA gene sequence analysis, strain AS-1 was designated as Enterobacter sp. AS-1.
• Cell-density dependent anammox activity of Candidatus Brocadia sinica regulated by N-acyl homoserine lactone-mediated quorum sensing
  Mamoru Oshiki; Haruna Hiraizumi; Hisashi Satoh; Satoshi Okabe
  Microbes Environ., 35, 4, ME20086, Aug. 2020, [Peer-reviewed], [Lead author, Corresponding author], [Domestic magazines]
  English, Scientific journal, The activity of anaerobic ammonia-oxidizing (anammox) bacteria is considered to depend on cell density; however, this has not yet been confirmed due to the fastidious nature of anammox bacteria (e.g., slow growth, oxygen sensitivity, and rigid aggregate formation). In the present study, the cell density-dependent occurrence of anammox activity (14-15N2 gas production rate) was investigated using planktonic enrichment cultures of Candidatus Brocadia sinica. This activity was detectable when the density of cells was higher than 107‍ ‍cells‍ ‍mL-1 and became stronger with increases in cell density. At the cell densities, the transcription of the BROSI_A1042 and BROSI_A3652 genes, which are potentially involved in the biosynthesis and reception of N-acyl homoserine lactone (AHL), was detectable in Brocadia sinica cells. The presence of AHL molecules in the MBR culture of B. sinica was confirmed by an AHL reporter assay and gas chromatography mass spectrometry analysis. The exogenous addition of the MBR culture extract and AHL molecules (a cocktail of C6, C8, C10, and C12-homoserine lactones) increased the specific 14-15N2 production rate of B. sinica. These results suggest that the specific anammox activity of B. sinica is regulated by AHL-mediated quorum sensing.
• N₂O production using native nos-deficient denitrifying bacterial strains screened by a genome mining approach
  Mamoru Oshiki; Miho Ishimaru; Masashi Hatamoto; Takashi Yamaguchi; Nobuo Araki; Satoshi Okabe
  Bioresour. Technol. Rep., 11, 100529, Aug. 2020, [Peer-reviewed], [Lead author, Corresponding author]
• Formation of denitrifying granules in an upflow sludge blanket reactor with municipal sewage and sodium nitrate feeding
  Takahiro Watari; Wilasinee Kotcharoen; Takanori Omine; Masashi Hatamoto; Nobuo Araki; Mamoru Oshiki; Kazuhisa Mimura; Akihiro Nagano; Takashi Yamaguchi
  Environ. Technol. Innov., 19, 100861, 100861, Elsevier BV, Aug. 2020, [Peer-reviewed]
  Scientific journal
• 異なる温度条件下における海洋性Anammox細菌の脱アンモニウム能
  田中孝国; 柴田真輝; フーレイチア; 田中昭雄; 押木守; 金田一智規; 新田見匡
  工業用水, 661, 69, 75, Jul. 2020, [Peer-reviewed]
• Biosynthesis of hydrazine from ammonium and hydroxylamine using an anaerobic ammonium oxidizing bacterium
  Mamoru Oshiki; Koshiro Yamada; Itsuki Kato; Kento Okoshi; Toshiro Imai; Takashi Yamaguchi; Nobuo Araki
  Biotechnology Letters, 42, 6, 979, 985, Springer Science and Business Media {LLC}, 17 Jun. 2020, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Simple and reliable enumeration of Escherichia coli concentrations in wastewater samples by measuring β-D-glucuronidase (GUS) activities via a microplate reader
  Hisashi Satoh; Kai Kikuchi; Yutaka Katayose; Shu Tsuda; Reiko Hirano; Yuga Hirakata; Masaaki Kitajima; Satoshi Ishii; Mamoru Oshiki; Masashi Hatamoto; Masahiro Takahashi; Satoshi Okabe
  Sci. Total Environ., 715, 136928, 01 May 2020, [Peer-reviewed]
  Scientific journal
• Food selectivity of anaerobic protists and direct evidence for methane production using carbon from prey bacteria by endosymbiotic methanogen.
  Yuga Hirakata; Masashi Hatamoto; Mamoru Oshiki; Takahiro Watari; Nobuo Araki; Takashi Yamaguchi
  ISME J., 14, 7, 1873, 1885, 27 Apr. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Anaerobic protists are major predators of prokaryotes in anaerobic ecosystems. However, little is known about the predation behavior of anaerobic protists because almost none have been cultured. In particular, these characteristics of anaerobic protists in the phyla Metamonada and Cercozoa have not been reported previously. In this study, we isolated three anaerobic protists, Cyclidium sp., Trichomitus sp., and Paracercomonas sp., from anaerobic granular sludge in an up-flow anaerobic sludge blanket reactor used to treat domestic sewage. Ingestion and digestion of food bacteria by anaerobic protists with or without endosymbiotic methanogens were demonstrated using tracer experiments with green fluorescent protein and a stable carbon isotope. These tracer experiments also demonstrated that Cyclidium sp. supplied CO2 and hydrogen to endosymbiotic methanogens. While Cyclidium sp. and Trichomitus sp. ingested both Gram-negative and -positive bacteria, Paracercomonas sp. could only take up Gram-negative bacteria. Archaeal cells such as Methanobacterium beijingense and Methanospirillum hungatei did not support the growth of these protists. Metabolite patterns of all three protists differed and were influenced by food bacterial species. These reported growth rates, ingestion rates, food selectivity, and metabolite patterns provide important insights into the ecological roles of these protists in anaerobic ecosystems.
• Total ammonia nitrogen (TAN) removal performance of a recirculating down-hanging sponge (DHS) reactor operated at 10 to 20ºC with activated carbon
  Mamoru Oshiki; Takashi Aizuka; Hirotoshi Netsu; Satoshi Oomori; Akihiro Nagano; Takashi Yamaguchi; Nobuo Araki
  Aquaculture, 520, 734963, Jan. 2020, [Peer-reviewed], [Lead author, Corresponding author]
• Draft genome sequence of Deinococcus sp. KR-1, a potential strain for palladium-leaching
  Hironaga Akita; Yuya Itoiri; Akio Kumagai; Noriyo Takeda; Akinori Matsushika; Mamoru Oshiki; Zen-ichiro Kimura
  J. Genomics, 8, 21, 24, Ivyspring International Publisher, Jan. 2020, [Peer-reviewed]
  Scientific journal
• Temporal variation of eukaryotic community structures in UASB reactor treating domestic sewage as revealed by 18S rRNA gene sequencing
  Yuga Hirakata; Masashi Hatamoto; Mamoru Oshiki; Takahiro Watari; Kyohei Kuroda; Nobuo Araki; Takashi Yamaguchi
  Sci. Rep., 9, 1, 12783, 12783, 01 Dec. 2019, [Peer-reviewed]
  English, Scientific journal
• Draft Genome Sequence of the Plant Growth-Promoting Streptomyces sp. Strain 6-11-2
  Ayman Ztouti; Natsumi Saito; Junna Sasaki; Takashi Yamaguchi; Nobuo Araki; Mamoru Oshiki
  Microbiology Resource Announcements, 8, e00877-19, 21 Nov. 2019, [Peer-reviewed], [Last author, Corresponding author]
  Scientific journal
• Thiocyanate degradation by a highly enriched culture of the neutrophilic halophile Thiohalobacter sp. strain FOKN1 from activated sludge and genomic insights into thiocyanate metabolism
  Oshiki M; Fukushima T; Kawano S; Kasahara Y; Nakagawa J
  Microbes Environ., 34, 4, 402, 412, Japanese Society of Microbial Ecology, Oct. 2019, [Peer-reviewed], [Lead author, Corresponding author]
  Scientific journal
• Dual nitrogen and oxygen isotope fractionation during anaerobic ammonium oxidation by anammox bacteria
  Kanae Kobayashi; Akiko Makabe; Midori Yano; Mamoru Oshiki; Tomonori Kindaichi; Karen L. Casciotti; Satoshi Okabe
  ISME J., 13, 10, 2426, 2436, Springer Science and Business Media LLC, Oct. 2019, [Peer-reviewed]
  Scientific journal
• Ammonium removal ability of marine Anammox bacteria under the different salinity environment
  田中 孝国; 半田 佳幹; フーレイチア; 田中 昭雄; 押木 守; 金田一 智規; 新田見 匡
  工業用水 = Journal of industrial water, 656, 656, 77, 84, 日本工業用水協会, Sep. 2019, [Peer-reviewed]
  Japanese, Scientific journal
• Investigation of prospective factors that control Kouleothrix (Type 1851) filamentous bacterial abundance and their correlation with sludge settleability in full-scale wastewater treatment plants
  Nittami, T; Shoji, T; Koshiba, Y; Noguchi, M; Oshiki, M; Kuroda, M; Kindaichi, T; Fukuda, J; Kurisu F
  Process Saf. Environ., 124, 137, 142, Apr. 2019, [Peer-reviewed]
  Scientific journal
• Effects of up-flow velocity on the community of anaerobic protozoa in UASB fed with municipal sewage
  YOSHIDA Chikara; SATO Takeshi; OSHIKI Mamoru; ARAKI Nobuo; HIRAKATA Yuga; HATAMOTO Masashi; YAMAGUCHI Takashi
  Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research), 75, 7, III_19, III_24, Japan Society of Civil Engineers, 2019, [Peer-reviewed]
  Japanese, Scientific journal,

   In this study, the up-flow velocity in the UASB reactor fed with municipal sewage was changed stepwise, and the influence on number of anaerobic protozoan cells and protozoan community was measured. Number of protozoan cells increased by rising the up-flow velocity in the range of 2.4 to 14.4 m/day, but the number of the ciliates decreased sharply due to a washout occurred at 22.4 m/day. Predominant species of anaerobic protozoa present in UASB were Metopus palaeformis, Metopus contortus, Chaenomorpha sp., and Plagiopyla sp, which accounted for about 60% of all 18S rRNA genes. Dominant species of protozoa changed in a short period of time under higher up-flow velocity conditions.

• Health impact of agricultural drainage water for farmers in the West Nile Delta
  Okubo, T; Iguchi, A; Tanaka, S; Uchida, S; Tagawa, T; Oshiki, M; Araki, A; Tawfik, A; Takahashi, M; Kubota, K; Harada, H; Uemura, S
  Int. J. Environ. Res, 13, 319, 325, 2019, [Peer-reviewed]
  English, Scientific journal
• Synergistic inhibition of anaerobic ammonium oxidation (anammox) activity by phenol and thiocyanate
  Mamoru Oshiki; Yoshiko Masuda; Takashi Yamaguchi; Nobuo Araki
  Chemosphere, 213, 498, 506, Elsevier {BV}, Dec. 2018, [Peer-reviewed], [Lead author, Corresponding author]
  Scientific journal
• Nitric oxide production from nitrite reduction and hydroxylamine oxidation by copper-containing dissimilatory nitrite reductase (nirK) from the aerobic ammonia-oxidizing archaeon, Nitrososphaera viennensis
  Kobayashi S; Hira D; Yoshida K; Toyofuku M; Shida Y; Ogasawara W; Yamaguchi T; Araki N; Oshiki M
  Microbes Environ., 33, 4, 428, 434, Japanese Society of Microbial Ecology, Oct. 2018, [Peer-reviewed], [Last author, Corresponding author]
  Scientific journal
• 窒素を食べる微生物たち
  押木守
  生物工学会誌, 96, 7, 408, 25 Jul. 2018, [Lead author, Corresponding author]
  Japanese
• Ureolytic prokaryotes in soil: community abundance and diversity
  Mamoru Oshiki; Mitsuru Araki; Yuga Hirakata; Masashi Hatamoto; Takashi Yamaguchi; Nobuo Araki
  Microbes Environ., 33, 2, 230, 233, Jul. 2018, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Startup of Marine Anammox bacteria reactor using Non-woven sheet
  田中孝国; CHIA Foo Lay; 田中昭雄; 押木守; 金田一智規; 新田見匡
  工業用水, 648, 648, 39, 43, 日本工業用水協会, May 2018, [Peer-reviewed]
  Japanese, Scientific journal
• Experimental evidence for in situ nitric oxide production in anaerobic ammonia-oxidizing bacterial granules
  Rathnayake, Rathnayake M. L. D.; Oshiki, Mamoru; Ishii, Satoshi; Segawa, Takahiro; Satoh, Hisashi; Okabe, Satoshi
  Environ. Sci. Technol., 52, 10, 5744, 5752, May 2018, [Peer-reviewed]
  English, Scientific journal
• Microfluidic PCR amplification and MiSeq amplicon sequencing techniques for high-throughput detection and genotyping of human pathogenic RNA viruses in human feces, sewage, and oysters
  Mamoru Oshiki; Takayuki Miura; Shinobu Kazama; Takahiro Segawa; Satoshi Ishii; Masashi Hatamoto; Takashi Yamaguchi; Kengo Kubota; Akinori Iguchi; Tadashi Tagawa; Tsutomu Okubo; Shigeki Uemura; Hideki Harada; Naohiro Kobayashi; Nobuo Araki; Daisuke Sano
  Front. Microbiol., 9, 830, Frontiers Media S.A., 27 Apr. 2018, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Nitrogen cycle evaluation (NiCE) chip for simultaneous analysis of multiple N cycle associated genes
  Mamoru Oshiki; Takahiro Segawa; Satoshi Ishii
  Appl. Environ. Microbiol., 84, 8, e02615-17, American Society for Microbiology, 01 Apr. 2018, [Peer-reviewed], [Lead author]
  English, Scientific journal
• エジプトの高校生を対象とした「水」に関する環境教育プログラムの開発と実践 (下水道協会誌論文集)
  大久保 努; 上村 繁樹; 井口 晃徳; 多川 正; 押木 守; 久保田 健吾; 荒木 信夫; 原田 秀樹; Tawfik Ahmed; 市村 禎二郎
  下水道協会誌 = Journal of Japan Sewage Works Association, 55, 665, 76, 82, 日本下水道協会, Mar. 2018, [Peer-reviewed]
  Japanese
• Microbial Community Analysis Revisited : Transition and Prospect
  伊藤 司; 金田一 智則; 押木 守; 岡部 聡
  EICA : journal of EICA : 環境システム計測制御学会誌, 22, 4, 21, 26, 環境システム計測制御学会, 2018, [Peer-reviewed]
  Japanese
• Development of a novel disinfection method for agricultural reuse of sewage treated by the primary sedimentation + DHS system
  KAGEMASA Shuka; NAGAMACHI Akihiro; IGUCHI Akinori; KUBOTA Kengo; TAKAHASHI Masanobu; HARADA Hideki; OSHIKI Mamoru; ARAKI Nobuo; OKUBO Tsutomu; UEMURA Shigeki; TAGAWA Tadashi
  Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research), 74, 7, 333, 340, Japan Society of Civil Engineers, 2018, [Peer-reviewed]
  Japanese, Scientific journal, Arid countries are facing serious water shortage, and sewage without sufficient treatment is often used for irrigation in some countries such as Egypt. A novel sewage treatment system, a down-flow hanging sponge (DHS) reactor combined with a primary sedimentation tank (PS+DHS system), has been proposed to overcome this issue, but until today its treatment efficiency has not met the Egyptian irrigation standards in terms of fecal coliform (≦5×10³ MPN/100mL). In this study, hydraulic retention time of the DHS reactor was prolonged to 4.8 hours; nevertheless, it was still not able to meet the irrigation standards. Disinfection with copper ion was evaluated, and it was found that the treatment with 2 mg/L of copper ion for at least 2 hours was necessary to achieve the irrigation standards. Growth inhibition and copper ion accumulation were not observed when copper ion-treated water was used for tomato cultivation.
• Microbial competition among anammox bacteria in nitrite-limited bioreactors
  Lei Zhang; Yuko Narita; Lin Gao; Muhammad Ali; Mamoru Oshiki; Satoshi Ishii; Satoshi Okabe
  Water Res., 125, 249, 258, Nov. 2017, [Peer-reviewed]
  English, Scientific journal
• Genetic diversity of marine anaerobic ammonium-oxidizing bacteria as revealed by genomic and proteomic analyses of "Candidatus Scalindua japonica'
  Mamoru Oshiki; Keisuke Mizuto; Zen-ichiro Kimura; Tomonori Kindaichi; Hisashi Satoh; Satoshi Okabe
  Environ. Microbiol. Rep., 9, 5, 550, 561, Oct. 2017, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Draft genome sequence of Thiohalobacter thiocyanaticus strain FOKN1, a neutrophilic halophile capable of thiocyanate degradation
  Mamoru Oshiki; Toshikazu Fukushima; Shuichi Kawano; Junichi Nakagawa
  Genome Announc., 5, 32, e00799-17, American Society for Microbiology, 01 Aug. 2017, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Improvement of a Phosphate Ion-selective Microsensor Using Bis(dibromophenylstannyl)methane as a Carrier
  Hisashi Satoh; Yuji Miyazaki; Shou Taniuchi; Mamoru Oshiki; Rathnayake M. L. D. Rathnayake; Masahiro Takahashi; Satoshi Okabe
  Anal. Sci., 33, 7, 825, 830, Jul. 2017, [Peer-reviewed]
  English, Scientific journal
• Maximum specific growth rate of anammox bacteria revisited
  Lei Zhang; Yuko Narita; Lin Gao; Muhammad Ali; Mamoru Oshiki; Satoshi Okabe
  Water Res., 116, 296, 303, Jun. 2017, [Peer-reviewed]
  English, Scientific journal
• Removal of human pathogenic viruses in a down-flow hanging sponge (DHS) reactor treating municipal wastewater and health risks associated with utilization of the effluent for agricultural irrigation
  Naohiro Kobayashi; Mamoru Oshiki; Toshihiro Ito; Takahiro Segawa; Masashi Hatamoto; Tsuyoshi Kato; Takashi Yamaguchi; Kengo Kubota; Masanobu Takahashi; Akinori Iguchi; Tadashi Tagawa; Tsutomu Okubo; Shigeki Uemura; Hideki Harada; Toshiki Motoyama; Nobuo Araki; Daisuke Sano
  Water Res., 110, 389, 398, Mar. 2017, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Eukaryotic community in UASB reactor treating domestic sewage based on 18S rRNA gene sequencing
  Y. Hirakata; M. Hatamoto; M. Oshiki; N. Araki; T. Yamaguchi
  Lecture Notes in Civil Engineering, 4, 218, 224, 2017, [Peer-reviewed]
  English, In book
• Ecology and physiology of anaerobic ammonium oxidizing bacteria
  Mamoru Oshiki; Hisashi Satoh; Satoshi Okabe
  Environ. Microbiol., 18, 9, 2784, 2796, Sep. 2016, [Peer-reviewed], [Lead author]
  English
• Hydroxylamine-dependent anaerobic ammonium oxidation (anammox) by "Candidatus Brocadia sinica"
  Mamoru Oshiki; Muhammad Ali; Kaori Shinyako-Hata; Hisashi Satoh; Satoshi Okabe
  Environ. Microbiol., 18, 9, 3133, 3143, Sep. 2016, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Effects of predation by protists on prokaryotic community function, structure, and diversity in anaerobic granular sludge
  Yuga Hirakata; Mamoru Oshiki; Kyohei Kuroda; Masashi Hatamoto; Kengo Kubota; Takashi Yamaguchi; Hideki Harada; Nobuo Araki
  Microbes Environ., 31, 3, 279, 287, Sep. 2016, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Removal of sanitarian microbial indicators after the combined process of a primary sedimentation and a down-flow hanging sponge (PS+DHS system)
  NAGAMACHI Akihiro; IGUCHI Akinori; SETO Yuta; KUBOTA Kengo; OSHIKI Mamoru; ARAKI Nobuo; OKUBO Tsutomu; UEMURA Shigeki; TAKAHASI Masanobu; HARADA Hideki; TAGAWA Tadashi
  Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research), 72, 7, III_187, III_195, 公益社団法人 土木学会, 2016, [Peer-reviewed]
  Japanese, &nbsp;A Down-flow Hanging Sponge (DHS) reactor combined with a primary sedimentation tank (PS+DHS system) was operated over 1,000 days to investigate the effect of HRT on treatment efficiencies. Insufficient effluent water quality was obtained when an HRT was set at 0.8 hours for DHS. After elongating HRT to 3.2 hours, high effluent water quality in terms of organic matter removals (>90% for BOD and SS) and microbial indicators (2.73 log for F.coli and 3.15 log for T.coli) was achieved. A removal efficiencies of sanitarian microbial indicators of an activated sludge process treating the same sewage were 2.55 log for F.coli and 2.19 log for T.coli, indicating that the PS+DHS system has comparable removal efficiencies to the activated sludge process. From the result obtained, the PS+DHS system with longer HRT (3.2 hours) can be an alternative process of an activated sludge process for sewage treatment.
• 下水再生処理におけるヒト腸管系ウイルスの目標除去効率の算定法
  伊藤寿宏; 押木守; 小林直央; 加藤毅; 瀬川高弘; 幡本将史; 山口隆司; 原田秀樹; 北島正章; 岡部聡; 佐野大輔
  土木学会論文集 G(環境)(Web), 72, 7, III_305‐III_313(J‐STAGE), III_313, 公益社団法人 土木学会, 2016, [Peer-reviewed]
  Japanese, &nbsp;The present study proposes an approach for determining a target value of enteric virus removal in wastewater reclamation processes based on monitoring datasets of source water quality and tolerable annual disease burden. In the proposed approach, probabilistic distributions of norovirus (NoV) concentration in the influent of domestic wastewater treatment plant were estimated, and tolerable concentration of NoV in reclaimed wastewater was determined to meet the tolerable annual disease burden (10^-6 disability adjusted life year per person per year) based on quantitative microbial risk assessment (QMRA). The target values of NoV removal were then computed using the probabilistic distributions of NoV and the tolerabole concentration according to six exposure scenarios for water reuse. It is required to monitor the virus concentration in untreated wastewater even after the operation of wastewater reclamation and reuse system is started. It is also important to continusouly update models and assumptions in QMRA for revising locally appropriate level of performance target value of enteric virus removal in a wastewater reclamation system.
• Draft genome sequence of an anaerobic ammonium-oxidizing bacterium, "Candidatus Brocadia sinica"
  Mamoru Oshiki; Kaori Shinyako-Hata; Hisashi Satoh; Satoshi Okabe
  Genome Announc., 3, 2, e00267-15, American Society for Microbiology, 2016, [Peer-reviewed], [Lead author]
  English, Scientific journal
• High-cell-density cultivation of Nitrosomonas europaea in a membrane bioreactor for performing protein purification and characterization studies
  Mamoru Oshiki; Ryota Takagi; Masashi Hatamoto; Takashi Yamaguchi; Nobuo Araki
  J. Gen. Appl. Microbiol., 62, 6, 330, 333, 2016, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Effects of dissolved oxygen and pH on nitrous oxide production rates in autotrophic partial nitrification granules
  Rathnayake M. L. D. Rathnayake; Mamoru Oshiki; Satoshi Ishii; Takahiro Segawa; Hisashi Satoh; Satoshi Okabe
  Bioresour. Technol., 197, 15, 22, Dec. 2015, [Peer-reviewed]
  English, Scientific journal
• Identification and detection of prokaryotic symbionts in the ciliate Metopus from anaerobic granular sludge
  Yuga Hirakata; Mamoru Oshiki; Kyohei Kuroda; Masashi Hatamoto; Kengo Kubota; Takashi Yamaguchi; Hideki Harada; Nobuo Araki
  Microbes Environ., 30, 4, 335, 338, Dec. 2015, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Rapid and successful start-up of anammox process by immobilizing the minimal quantity of biomass in PVA-SA gel beads
  Muhammad Ali; Mamoru Oshiki; Lashitha Rathnayake; Satoshi Ishii; Hisashi Satoh; Satoshi Okabe
  Water Res., 79, 147, 157, Aug. 2015, [Peer-reviewed]
  English, Scientific journal
• Physiological characterization of anaerobic ammonium oxidizing bacterium "CandidatusJettenia caeni'
  Muhammad Ali; Mamoru Oshiki; Takanori Awata; Kazuo Isobe; Zenichiro Kimura; Hiroaki Yoshikawa; Daisuke Hira; Tomonori Kindaichi; Hisashi Satoh; Takao Fujii; Satoshi Okabe
  Environ. Microbiol., 17, 6, 2172, 2189, Jun. 2015, [Peer-reviewed]
  English, Scientific journal
• Efficient Nitrogen Removal by Anaerobic Ammonium Oxidation (anammox) Process
  押木 守; 佐藤 久; 岡部 聡
  Journal of environmental biotechnology, 14, 1, 21, 29, 環境バイオテクノロジー学会, Oct. 2014, [Peer-reviewed], [Lead author]
  Japanese
• Simple, rapid and effective preservation and reactivation of anaerobic ammonium oxidizing bacterium "Candidatus Brocadia sinica"
  Muhammad Ali; Mamoru Oshiki; Satoshi Okabe
  Water Res., 57, 215, 222, Jun. 2014, [Peer-reviewed]
  English, Scientific journal
• Source identification of nitrous oxide on autotrophic partial nitrification in a granular sludge reactor
  R. M. L. D. Rathnayake; Y. Song; A. Tumendelger; M. Oshiki; S. Ishii; H. Satoh; S. Toyoda; N. Yoshida; S. Okabe
  Water Res., 47, 19, 7078, 7086, Dec. 2013, [Peer-reviewed]
  English, Scientific journal
• Cultivation of Planktonic Anaerobic Ammonium Oxidation (Anammox) Bacteria Using Membrane Bioreactor
  Oshiki, Mamoru; Awata, Takanori; Kindaichi, Tomonori; Satoh, Hisashi; Okabe, Satoshi
  Microbes Environ., 28, 4, 436, 443, Dec. 2013, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Nitrate-dependent ferrous iron oxidation by anaerobic ammonium oxidation (anammox) bacteria
  M. Oshiki; S. Ishii; K. Yoshida; N. Fujii; M. Ishiguro; Hisashi Satoh; S. Okabe
  Appl. Environ. Microbiol., 79, 13, 4087, 4093, Jul. 2013, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Physiological Characterization of an Anaerobic Ammonium-Oxidizing Bacterium Belonging to the "Candidatus Scalindua" Group
  Takanori Awata; Mamoru Oshiki; Tomonori Kindaichi; Noriatsu Ozaki; Akiyoshi Ohashi; Satoshi Okabe
  Appl. Environ. Microbiol., 79, 13, 4145, 4148, Jul. 2013, [Peer-reviewed]
  English, Scientific journal
• Microbial community composition of polyhydroxyalkanoate-accumulating organisms in full-scale wastewater treatment plants operated in fully aerobic mode
  Mamoru Oshiki; Motoharu Onuki; Hiroyasu Satoh; Takashi Mino
  Microbes Environ., 28, 1, 96, 104, Mar. 2013, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Revealing microbial community structures in large- and small-scale activated sludge systems by barcoded pyrosequencing of 16S rRNA gene
  Purnika Damindi Ranasinghe; Hiroyasu Satoh; Mamoru Oshiki; Kenshiro Oshima; Wataru Suda; Masahira Hattori; Takashi Mino
  Water Sci. Technol., 66, 10, 2155, 2161, 2012, [Peer-reviewed]
  English, Scientific journal
• N₂O emission from a partial nitrification-anammox process and identification of a key biological process of N₂O emission from anammox granules
  Satoshi Okabe; Mamoru Oshiki; Yoshitaka Takahashi; Hisashi Satoh
  Water Res., 45, 19, 6461, 6470, Dec. 2011, [Peer-reviewed]
  English, Scientific journal
• Development of long-term stable partial nitrification and subsequent anammox process
  Satoshi Okabe; Mamoru Oshiki; Yoshitaka Takahashi; Hisashi Satoh
  Bioresour. Technol., 102, 13, 6801, 6807, Jul. 2011, [Peer-reviewed]
  English, Scientific journal
• Physiological characteristics of the anaerobic ammonium-oxidizing bacterium "Candidatus Brocadia sinica"
  Mamoru Oshiki; Masaki Shimokawa; Naoki Fujii; Hisashi Satoh; Satoshi Okabe
  Microbiology, 157, 6, 1706, 1713, Jun. 2011, [Peer-reviewed], [Lead author]
  English, Scientific journal
• 微生物の多様性―「遺伝子の多様性」/「細胞の多様性」から見た生物多様性 バイオフィルム
  押木守; 岡部聡
  生物の科学 遺伝, 65, 3, 48, 54, エヌ・ティー・エス, 01 May 2011, [Lead author]
  Japanese
• Rapid quantification of polyhydroxyalkanoates (PHA) concentration in activated sludge with the fluorescent dye Nile blue A
  M. Oshiki; H. Satoh; T. Mino
  Water Sci. Technol., 64, 3, 747, 753, 2011, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• A great leap forward in microbial ecology
  Satoshi Okabe; Mamoru Oshiki; Yoichi Kamagata; Nobuyasu Yamaguchi; Masanori Toyofuku; Yutaka Yawata; Yosuke Tashiro; Nobuhiko Nomura; Hiroyuki Ohta; Moriya Ohkuma; Akira Hiraishi; Kiwamu Minamisawa
  Microbes Environ., 25, 4, 230, 240, Dec. 2010, [Peer-reviewed]
  English
• Separation of PHA-accumulating cells in activated sludge based on differences in buoyant density
  Mamoru Oshiki; Motoharu Onuki; Hiroyasu Satoh; Takashi Mino
  J. Gen. Appl. Microbiol., 56, 2, 163, 167, Apr. 2010, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Acetate uptake by PHA-accumulating and non-PHA-accumulating organisms in activated sludge from an aerobic sequencing batch reactor fed with acetate
  M. Oshiki; H. Satoh; T. Mino
  Water Sci. Technol., 62, 1, 8, 14, 2010, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Temporal Carbon Storage in Activated Sludge during the Removal of Organic Pollutants in Fully Aerobic Wastewater Treatment Plants
  OSHIKI Mamoru; SATOH Hiroyasu; ONUKI Motoharu; MINO Takashi
  Journal of Japan Sewage Works Association, 46, 566, 126, 137, 日本下水道協会, 15 Dec. 2009, [Peer-reviewed], [Lead author, Corresponding author]
  Japanese, Scientific journal
• 微生物の持つ有機物貯蔵能力を利用した省エネルギー型廃水処理法の提案
  押木 守; 佐藤 弘泰; 味埜 俊
  用水と廃水, 51, 41, 49, 2009, [Peer-reviewed], [Lead author, Corresponding author]
  Japanese, Scientific journal
• Occurrence of polyhydroxyalkanoate as temporal carbon storage material in activated sludge during the removal of organic pollutants
  Oshiki, M; Yang, Y; Onuki, M; Satoh, H; Peng, Y.-Z; Mino, T
  J. Water Environ. Technol, 6, 2, 77, 83, Japan Society on Water Environment, 2008, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal, The amounts of temporal carbon storage material, polyhydroxyalkanoate (PHA), were quantified in the course of wastewater treatment by different activated sludge reactors operated at Beijing University of Technology, Beijing, China. The studied reactors were five continuous ones and two sequencing batch ones. In all the reactors, PHA was detected, and its contents within activated sludge ranged between 0.2% and 3.2%. Soluble organic matters loaded to the reactors were mainly removed in the first anaerobic or microaerophilic tank and one fifth to all of removed soluble organic matters were tentatively stored as PHA in the studied reactors. It was concluded that not a small part of soluble organic matters in sewage is converted to PHA in the course of their removal by activated sludge.
• PHA-accumulating microorganisms in full-scale wastewater treatment plants
  Mamoru Oshiki; Motoharu Onuki; Hiroyasu Satoh; Takashi Mino
  Water Sci. Technol., 58, 1, 13, 20, 2008, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Contribution of PHA-accumulating Organisms to Acetate Uptake by Activated Sludge Microorganisms
  OSHIKI Mamoru; ONUKI Motoharu; Satoh Hiroyasu; MINO Takashi
  Doboku Gakkai Ronbunshuu G, 43, 195, 203, Japan Society of Civil Engineers, 17 Nov. 2006, [Peer-reviewed], [Lead author, Corresponding author]
  Japanese, Scientific journal, The contribution of PHA-accumulating organisms to acetate uptake was estimated by the batch experiments using activated sludge sample taken from full-scale wastewater treatment plant and dual staining of Nile blue A and DAPI was applied to activated sludge sample in order to evaluate the abundance of PHA-accumulating organisms. The results suggested that 20 to almost 100% of acetate had been taken by the member of Nile blue A positive cells and their population accounted for 10 to 20% in activate sludge incubated under aerobic condition. In order to estimate the contribution of <I>Candidatus</I> "Accumulibacter phosphatis" and <I>Candidatus</I> "Competibacter phosphatis" to acetate uptake, their population were quantified by fluorescent in-situ hybridization (FISH) analysis, and they accounted for 10 to 90% of population of Nile blue A positive cells.
• Effects of dissolved oxygen concentration and C/N ratio on transcription level of nitrite reductase gene in denitrifying sludge
  OSHIKI Mamoru; ARAKI Nobuo; TAKEBAYASHI Satoru; NAGANO Akihiro
  Journal of Japan Society on Water Environment, 28, 11, 683, 687, 公益社団法人 日本水環境学会, 10 Nov. 2005, [Peer-reviewed], [Lead author]
  Japanese, Scientific journal, The transcription level of the nitrite reductase gene of denitrifying bacteria in a lab-scale denitrifying reactor was investigated by real-time reverse transcription (RT)-PCR technique as an indicator of in-situ microbial activity. Two types of batch experiment were conducted to clarify the effects of dissolved oxygen (DO) and carbon/nitrogen (C/N) ratio on the relative nirS mRNA level in denitrifying sludge. The reverse transcription of total RNA with the primer, nirS 6R, specific for the nirS gene was suitable for the subsequent quantification of the nirS gene by real-time PCR. The presence of DO and a low C/N ratio in the medium resulted in a rapid decrease in the relative transcription level of nirS mRNA from 105 to 103 copies·ng-1 total RNA within four hours of the batch experiments. The relative transcription remained at the same level after nitrate concentration decreased to a level low enough to reduce denitrification rate. Although there was no relationship between the denitrification rate of the reactor and the relative transcription level of nirS mRNA in the batch experiments, a quick response of transcriptional induction of nirS mRNA corresponding to the changes in DO and C/N ratio was detected.
• Evaluation of the in-situ activity of ammonia-oxidizing population in biofilm by quantification of amoA mRNA
  OSHIKI Mamoru; ARAKI Nobuo; YAMAGUCHI Takashi; YAMAZAKI Shinichi; HARADA Hideki
  Doboku Gakkai Ronbunshuu G, 40, 63, 70, Japan Society of Civil Engineers, 14 Nov. 2003, [Peer-reviewed], [Lead author]
  Japanese, Scientific journal, The relationship between ammonia-oxidizing activities and <I>amoA</I> mRNA (encoding ammonia monooxigenase) levels was investigated by batch experiments with nitrifying biofilms taken from a lab-scale bioreactor. RT-PCR technology was applied to determine copy numbers of <I>amoA</I> mRNA in total RNA extracted from the sludge sample. Ammonia oxidation rates and transcription levels of <I>amoA</I> mRNA were monitored throughout the experiment that ammonia was fed as the concentration rose abruptly up to 20 or 100 mg-N/L whenever ammonia was consumed completely. A large ammonia turnover rate occurred immediately after every ammonia shock load, although the <I>amoA</I> mRNA level was still low. The turnover rate gradually reduced with decreasing ammonia concentration, while the <I>amoA</I> mRNA level increased until several hours after every ammonia shock load. The tendency of the <I>amoA</I> mRNA transcription changed a decrease as ammonia concentration dropped as low as Km value of <I>Nitrosomonas</I>. The induction of <I>amoA</I> mRNA was detected under the presence of low ammonia less than 5 mg-N/L. However the <I>amoA</I> mRNA level was not correlated with the ammonia turnover rate in the range from 5 to 100mg-N/L. It was indicated that the <I>amoA</I> mRNA level in the nitrifying sludge was not directly connected to the <I>in-situ</I> activity of ammonia-oxidizing bacteria.

• 間欠曝気運転が好気性脱窒細菌叢に与える影響
  上村光輝; 吉岡水輝; 土田 勝範; 川上周司; 渡利高大; 押木守; 多川 正; 荒木信夫, 第60回日本水環境学会年会 (2026年3月9日-11日) (L-061) 中央大学, 東京., Mar. 2026
• Nitrous oxide emission potential associated with nitrogen microbial community structure and activity of tropical biological nitrogen removal biomass
  Jia Xin Yong; Hyungmin Choi; Fairus Bin Rabuni; Faidzul Hakim Adnan; Satoshi Okabe; Mamoru Oshiki; Adeline Seak; May Chua, 第60回日本水環境学会年会 (2026年3月9日-11日) (P-6-10) 中央大学, 東京., Mar. 2026, [Corresponding author]
• 活性汚泥中における薬剤耐性遺伝子拡散への水平・垂直伝播機構の寄与
  髙橋 晃平; 小原 紀子; 樋口 宏介; 岡部 聡; 押木 守; 大村 拓也; David Johnson, 第60回日本水環境学会年会 (2026年3月9日-11日) (P-11-18) 中央大学, 東京., Mar. 2026, [Corresponding author]
• Horizontal and Vertical Gene Transfer of Antibiotic Resistance Genes in Activated Sludge
  Mamoru Oshiki, KMUTT–NUT–BIOTEC special symposium: advances in biotechnology and environmental engineering for sustainable societies, KMUTT Bangmod campus, Bangkok, Jan. 2026
• バイオものづくりに資する水素酸化細菌の獲得および特性評価
  押木 守; 崔 亨敏; 高橋 晃平; 岡部 聡; 王 謙, 第62回環境工学研究フォーラム、ポスター発表、2025年12月3日-12月5日、宮崎市民プラザ(宮崎), Dec. 2025
• 複合微生物系における機能性微生物群の統計的同定手法の開発
  押木 守; 福島 寿和; 川野 秀一; 中川 淳一, 2025 年度 日本農芸化学会北海道支部 第ニ回学術講演会 、北海道大学農学部(札幌), Nov. 2025
• Nitrous oxide emission associated with nitrogen metabolic activity and microbial community structure in tropical nitrogen removal biomass
  Hyungmin Choi; Jia Xin Yong; Fairus Bin Rabuni; Faidzul Hakim Adna; Satoshi Okabe; Mamoru Oshiki; Adeline Seak May Chu, 第32回衛生工学シンポジウム (2025年11月20日〜11月21日, 北海道大学, 北海道), Nov. 2025, [Corresponding author]
• 活性汚泥細菌叢中のプラスミド伝播ダイナミクスの解明
  髙橋晃平; 小原紀子; 樋口宏介; 大村拓也; 岡部聡; David Johnso, 第32回衛生工学シンポジウム (2025年11月20日〜11月21日, 北海道大学, 北海道), Nov. 2025, [Corresponding author]
• 中温・高温性水素酸化細菌の分離培養およびゲノム解析 –水素を用いたバイオものづくりを目指して–
  押木 守; 進藤 琉司; 崔 亨敏; 高橋 晃平; 岡部 聡; 王 謙, 第32回衛生工学シンポジウム (2025年11月20日〜11月21日, 北海道大学, 北海道), Nov. 2025, [Lead author]
• 嫌気性原生動物Cyclidiumとメタン菌の水素を介した細胞内共生
  平片 悠河; 延 優; 玉木 秀幸; 幡本 将史; 渡利 高大; 山口 隆司; 押木 守; 荒木 信夫, 日本共生生物学会第9回大会 (2025年11月28日〜12月1日, 名桜大学, 沖縄), Nov. 2025
• Stability and resistance of mainstream anammox granules under combined low-temperature and organic stress
  Hyungmin Choi; Jihun Park; Huiju Park; Mamoru Oshiki; Satoshi Okabe; Changsoo Lee, Korean Society of Environmental Engineers、2025年11月19日-11月21日、Jeju island, Nov. 2025
• Cold-adapted anammox granules enhance granule stability and nitrite utilization against denitrifying bacteria under mainstream organic loading stress
  Hyungmin Choi; Mamoru Oshiki; Satoshi Okabe; Changsoo Lee, 第28回日本水環境学会シンポジウム、ポスター発表、2025年9月17日-9月19日、富山県立大学射水キャンパス, Sep. 2025
• 微生物集団の機能解明に向けた統計的アプローチ:複雑系データ解析の新展開
  押木 守; 福島 寿和; 川野 秀一; 中川 淳一, 日本分析化学会第74年会、口頭発表 C1104S、2025年9月24日-9月26日、北海道大学工学部, Sep. 2025
• 細胞内共生メタン菌による水素除去が宿主原生動物に与える影響
  平片 悠河; 延 優; 玉木 秀幸; 幡本 将史; 渡利 高大; 山口 隆司; 押木 守; 荒木 信夫, 第58回日本原生生物学会大会、ポスター発表、2025年9月26日-9月28日、奈良教育大学, Sep. 2025
• Shewanella 由来の膜小胞による種特異的な脱窒促進機構の解明
  高橋 晃平; 武田 理久; トーマス サヴィチ; 片岡 柾人; 山本 達也; 岡部 聡; 野村 暢彦; 押木 守; 豊福 雅典; 徳納 吉秀, 日本微生物生態学会第38回東京大会、ポスター発表 P-034、2025年9月7日-9月10日、東京科学大学 大岡山キャンパス, Sep. 2025
• 温泉マット由来水素酸化細菌のゲノム解析
  押木 守; 崔 亨敏; 高橋 晃平; 岡部 聡; 王 謙, 日本微生物生態学会第38回東京大会、ポスター発表 P-034、2025年9月7日-9月10日、東京科学大学 大岡山キャンパス, Sep. 2025
• Kinetics and Structure of Nitrifying Communities in Tropical Wastewater Treatment Systems
  Yong J. X.; Oshiki M.; Rabuni F.; Adnan F. H.; Okabe S.; Takahashi K.; Chua A. S. M., The Water and Environment Technology Conference 2025 (WET2025), City Hall Plaza-Aôre (Nagaoka, Japan), 5–6 July 2025, Jul. 2025
• Cold-Endurance of Anammox Granules Reinforces Nitrite Utilization in Competition with Denitrifiers under Mainstream Organic Stress
  Choi H.; Park J.; Oshiki M.; Okabe S.; Lee C., The Water and Environment Technology Conference 2025 (WET2025), City Hall Plaza-Aôre (Nagaoka, Japan), 5–6 July 2025, Jul. 2025
• Core Microbiome in Mesophilic and Low-Temperature Partial Nitrification–Anammox Reactors: Structure, Distribution, and Roles
  Oshiki M.; Takahashi K.; Kawasaki S.; Choi H.; Park J.; Kim K.; Bae H.; Okabe S.; Lee C., The Water and Environment Technology Conference 2025 (WET2025), City Hall Plaza-Aôre (Nagaoka, Japan), 5–6 July 2025, Jul. 2025
• Species-Specific Enhancement of Denitrification by Shewanella Membrane Vesicles
  Takahashi K.; Takeda R.; Savage T. K.; Kataoka M.; Yamamoto T.; Okabe S.; Nomura N.; Oshiki M.; Toyofuku M.; Tokunou Y., The 15th Asian Symposium on Microbial Ecology (ASME2025), Chungbuk National University (Cheongju, South Korea), 28–30 March 2025, May 2025
• プラスミド接合伝達を利用した配列特異的DNA切断による薬剤耐性プラスミドの除去
  小原 紀子; 高橋 晃平; 岡部 聡; 押木 守, 第59回日本水環境学会年会 (2025年3月17日-19日) 北海道大学, 札幌., 3-I-11-2, Mar. 2025
• GFPタグプラスミドを用いた活性汚泥細菌叢中のプラスミド伝播ダイナミクスの解析
  高橋 晃平; 樋口 宏介; 徳田 真穂; 小原 紀子; 岡部 聡; 新谷 政己; 押木 守, 第59回日本水環境学会年会 (2025年3月17日-19日) 北海道大学, 札幌., 3-I-11-1, Mar. 2025
• Anammox反応における各窒素変換経路の可逆性
  河崎 聖也; 押木 守; 岡部 聡, 第59回日本水環境学会年会 (2025年3月17日-19日) 北海道大学, 札幌., 3-D-16-2, Mar. 2025
• 高温多湿な亜熱帯地域に適合する生物学的窒素除去技術の開発
  Leong Chew Lee; Rabuni Mohamad Fairus; Aris Alijah Mohd; Khor Bee Chin; Curtis Thomas; 高橋 晃平; 岡部 聡; 押木 守; Chua Adeline Seak May, 第59回日本水環境学会年会 (2025年3月17日-19日) 北海道大学, 札幌., 1-A-15-4, Mar. 2025
• Escherichia coliおよびShewanella oneidensisを用いたNH₃生成型亜硝酸還元酵素の発現
  板坂 紗花; 高橋 二千翔; 岡部 聡; 押木 守, 日本農芸化学会2025年度札幌大会 (2025年3月5日~8日) 札幌コンベンションセンター、札幌., 5A030, Mar. 2025
• Microbial ecology of low-dissolved oxygen biological nitrogen removal in tropical sewage treatment plants
  Jia Xing Loi; Liang Feng; Faidzul Hakim Adnan; Mohamad Fairus Rabuni; Bee Chin Khor; Alijah Mohd Aris; Mamoru Oshiki, Anton; Strunov; Stefan Thiele; Joana Séneca; Sarah Al-Ajeel; Ruizhe Pei; Petra Pjevac; Holger Daims; Adeline Seak May Chua, The 14th International Symposium on Southeast Asian Water Environment (SEAWE-14), 3rd December 2024, Kuala Lumpur, Malasia, A-108, Dec. 2024
• Hydrogen (H₂) and volatile fatty acids (VFA) production from bread wastes in thermophilic fermentation reaction
  Mamoru Oshiki; Genchi Yamaguchi; Kohei Takahashi; Satoshi Okabe; Shuichi Kawano; Junichi Nakagawa; Toshikazu Fukushima, The 14th International Symposium on Southeast Asian Water Environment (SEAWE-14), 3rd December 2024, Kuala Lumpur, Malasia, A-009, Dec. 2024
• 嫌気性原生動物の細胞内共生メタン菌への依存性
  平片 悠河; 延 優; 玉木 秀幸; 幡本 将史; 渡利 高大; 山口 隆司; 押木 守; 荒木 信夫, 日本微生物生態学会第37回広島大会、ポスター発表、2024年10月28日-10月31日、広島国際会議場、広島, Oct. 2024
• Bioelectrochemical NO3- reduction to NH4+ using NO3-/NO2- reductase of Escherichia coli and artificial electron mediator
  Nichika Takahashi; Mamoru Oshiki; Satoshi Okabe, The 14th Asian Symposium of Microbial Ecology (ASME), Poster, 18-20 September 2024, Taipei, Taiwan, Sep. 2024
• Conjugal transfer of an IncP-1 multidrug-resistance plasmid pB10: Horizontal and vertical gene transfer and the host range in activated sludge
  Kiko Ohara; Kosuke Higuchi; Kohei Takahashi; Satoshi Okabe; Mamoru Oshiki, The 14th Asian Symposium of Microbial Ecology (ASME), Poster WP14, 18-20 September 2024, Taipei, Taiwan, Sep. 2024
• Reversible nitrogen transformations in anaerobic ammonium oxidizing process
  Seiya Kawasaki; Mamoru Oshiki; Satoshi Okabe, The 14th Asian Symposium of Microbial Ecology (ASME), Poster MP26, 18-20 September 2024, Taipei, Taiwan, Sep. 2024
• Whole bacterial colony imaging using the clearing reagent and two-photon microscopy
  Takahashi Kohei; Okabe Satoshi; Oshiki Mamoru, The 14th Asian Symposium of Microbial Ecology (ASME), Poster FP10, 18-20 September 2024, Taipei, Taiwan, Sep. 2024
• Microbial community structure and functional potentials of the partial nitrification-anammox bioreactors
  Mamoru Oshiki; Hyungmin Choi; Jihye Park; Kohei Takahashi; Satoshi Okabe; Kwiyong Kim; Hyokwan Bae; Changsoo Lee, The 14th Asian Symposium of Microbial Ecology (ASME), Poster WP2, 18-20 September 2024, Taipei, Taiwan, Sep. 2024
• 嫌気性アンモニウム酸化反応における各窒素変換経路の可逆性
  河崎聖也; 押木守; 岡部聡, 第27回日本水環境学会シンポジウム、ポスター発表、岩手大学、盛岡、2024年9月11日-13日, Sep. 2024
• 嫌気性アンモニウム酸化細菌による亜硝酸酸化反応の窒素バックフラックス
  河崎聖也; 押木守; 岡部聡, 2024 年度 日本農芸化学会北海道支部 第一回学術講演会、ポスターP6、とかちプラザ, 帯広, 北海道, 2024年7月13日, Jul. 2024
• 可視光を用いた生物光化学的アプローチによる NH4+再生システムの開発
  進藤琉司; 押木守; 岡部聡, 2024 年度 日本農芸化学会北海道支部 第一回学術講演会、ポスターP16、とかちプラザ, 帯広, 北海道, 2024年7月13日, Jul. 2024
• 光エネルギーで駆動する生物光化学的NH₄⁺再生システムの開発
  進藤 琉司; 押木 守; 岡部 聡, 第58回日本水環境学会年会 L-070, Mar. 2024
• 活性汚泥に生息する従属栄養硝化・好気性脱窒細菌の特定
  上村 光輝; 川上 周司; 押木 守; 荒木 信夫, 第58回日本水環境学会年会 L-069, Mar. 2024
• Anammox細菌による亜硝酸酸化反応の窒素バックフラックス
  河崎 聖也; 押木 守; 岡部 聡, 第58回日本水環境学会年会 2-A-10-2, Mar. 2024
• 嫌気性アンモニア酸化(anammox)細菌の酸素排除メカニズム
  額田 啓志; 押木 守; 岡部 聡, 第58回日本水環境学会年会 2-A-10-1, Mar. 2024
• 微生物触媒を用いた高親和性かつ高効率な電気化学的NH₄⁺再生システムの開発
  髙橋 二千翔; 押木 守; 岡部 聡, 第58回日本水環境学会年会 1-C-15-3, Mar. 2024
• アナモックス細菌の酸素耐性メカニズムの解明
  額田 啓志; 押木 守; 岡部 聡, 日本微生物生態学会 第36回大会 (11月27日〜11月30日、浜松アクトシティ), P245-D, Nov. 2023
• 嫌気性アンモニウム酸化細菌における亜硝酸酸化-硝酸還元同時反応
  河崎 聖也; 押木 守; 岡部 聡, 日本微生物生態学会 第36回大会 (11月27日〜11月30日、浜松アクトシティ), P222-A, Nov. 2023
• 硝酸・亜硝酸還元酵素を還元触媒とする電気化学的なNH3再生プロセスの開発
  髙橋 二千翔; 押木 守; 岡部 聡, 日本微生物生態学会 第36回大会 (11月27日〜11月30日、浜松アクトシティ), P132-A, Nov. 2023
• Accumulation of nitrogen oxide intermediates during aerobic denitrification modulates the evolutionary potential of microbial populations
  Kohei Takahashi; Mamoru Oshiki; Chujin Ruan; Kana Morinaga; Masanori Toyofuku; Nobuhiko Nomura; David R. Johnson, 日本微生物生態学会 第36回大会 (11月27日〜11月30日、浜松アクトシティ), 2_43_O-p02, Nov. 2023
• Development of statistical method for identification of microorganisms responsible for wastewater treatment
  Toshikazu Fukushima; Shuichi Kawano; Mamoru Oshiki; Junichi Nakagawa, IWA Aspire Conference & Exhibition 2023, Oct. 2023
• Nitrite reduction to ammonium by anammox bacterial cell extracts
  M. Oshiki; T. Nakabayashi; A. Kamigaito; S. Okabe, 10th IWA Microbial Ecology and Water Engineering (MEWE) Specialist Conference (Brisbane, Australia, 10 September – 14 September) poster 104, Sep. 2023
• Remineralization of organic nitrogen compounds into ammonium in marine anammox bacterial culture
  Mamoru Oshiki; Emi Morimoto; Kanae Kobayashi; Hisashi Satoh; Satoshi Okabe, The Eighth International Conference on Nitrification and Related Processes (ICoN8) (Princeton University, USA, 30 July – 3 August), Session: Biotechnology/Applications, poster 309, Aug. 2023
• Oxygen tolerance and detoxification mechanisms of anammox bacteria
  Satoshi Okabe; Shaoyu Ye; Lan Xi; Keishi Nukada; Haozhe Zhang; Kanae Kobayashi; Mamoru Oshiki, The Eighth International Conference on Nitrification and Related Processes (ICoN8) (Princeton University, USA, 30 July – 3 August), Session: Biotechnology/Applications, poster 304, Aug. 2023
• Adsorption of green fluorescent protein-marked E. coli on activated sludge flocks
  Shota Ishizaki; Yusuke Ishizuka; Kosuke Higuchi; Mamoru Oshiki; Yuki Nakaya, Yuki; Hisashi Satoh, The 18th IWA Leading Edge Conference on Water and Wastewater Technologies (SESSION 5: EMERGING INNOVATIVE WASTEWATER TECHNOLOGIES P28), Jun. 2023
• Accumulation of intermediates during aerobic denitrification inhibits cell growth in low ph conditions
  K. Takahashi; M. Oshiki; N. Nomura; D. Johnson, ASM Microbe (Session P018 - AES10-1), Jun. 2023
• 生物電気化学的CO2還元有価物(酢酸)生産プロセスの構築
  糸入 祐也; 松尾 稜介; 押木 守; 岡部 聡, 第57回日本水環境学会年会 2-H-10-2, 210, 16 Mar. 2023
• 都市下水処理を対象とした活性汚泥に生息する好気性脱窒細菌の特定
  上村光輝; 川上周司; 渡利高大; 青木仁孝; 押木守; 荒木信夫, 第57回日本水環境学会年会 L-48, 588, 15 Mar. 2023
• 緑色蛍光タンパク質遺伝子導入大腸菌を用いた活性汚泥フロックの大腸菌吸着部位の解明
  石崎翔大; 石塚祐介; 樋口宏介; 押木守; 中屋佑紀; 佐藤久, 第57回日本水環境学会年会 L-38, 578, 15 Mar. 2023
• 海洋性Anammox細菌自動培養装置の作製
  土屋 美雨; 田中 孝国; 小林 康浩; 新田見 匡; 金田一 智規; 押木 守, 第25回化学工学会学生発表会, O33, 04 Mar. 2023
• アンモニア酸化細菌および脱窒菌由来の銅含有亜硝酸還元酵素NirKによる亜酸化窒素ハイブリッド生成反応の検討
  小林諒斗; 押木守; 佐藤久, 第59回環境工学研究フォーラム B41 (いわて県民情報交流センター、盛岡), Nov. 2022
• 回分式活性汚泥リアクターにおける薬剤耐性プラスミドの接合伝達頻度の解明
  樋口宏介; 北島正章; 岡部聡; 押木守, 日本微生物生態学会 第35回大会, PB-173, 01 Nov. 2022
• 嫌気性アンモニア酸化反応におけるヒドラジン分解・再合成(14N・15N組み分け)反応の検証
  額田 啓志; 押木 守; 岡部 聡, 日本微生物生態学会 第35回大会, PA-123, 01 Nov. 2022
• アンモニア酸化微生物のアンモニア酸化活性に伴う過酸化水素生成
  松本 衿花; 額田 啓志; Zhang Haozhe; 押木 守; 岡部 聡, 日本微生物生態学会 第35回大会, PA-21, 01 Nov. 2022
• 窒素安定同位体トレーサー試験を見据えた溶存態無機窒素化合物の15N/14N比の迅速測定技術の開発
  押木守; 永井孔明; 岡部聡, 令和4年度 農芸化学会 北海道・東北支部 合同支部会, E2, 21 Sep. 2022
• 活性汚泥における多剤耐性IncP-1群プラスミドpB10の伝播頻度・宿主域の解明
  樋口宏介; 北島正章; 岡部聡; 押木守, 令和4年度 農芸化学会 北海道・東北支部 合同支部会, E1, 21 Sep. 2022
• 完全なアンモニア酸化(COMAMMOX)細菌“Nitrospira inopinata”の窒素・酸素同位体分別の解析
  塚田 麦; 小林香苗; 押木 守; 岡部 聡, 第56回日本水環境学会年会, Mar. 2022
• 海洋性アナモックス細菌“Ca. Scalindua sp.”集積培養系における尿素及びシアン酸の代謝経路の解析
  森本衣美; 小林香苗; 押木 守; 岡部 聡, 第56回日本水環境学会年会, Mar. 2022
• AOB は電極呼吸によるアンモニア酸化が可能か?
  市原りえ; KOFFI Joel; 押木 守; 岡部 聡, 第56回日本水環境学会年会, Mar. 2022
• Growth of nitrite-oxidizing Nitrospira in marine nitrifying reactors: Bioenergetics and growth behaviors of marine Nitrospira and Nitrosomonas
  押木守; 根津拓福; 黒田恭平; 荒木信夫, 日本水環境学会シンポジウム講演集, 25th, 2022
• 海洋性Anammox細菌の最適な塩分濃度,温度,pH条件の検討
  田中孝国; 岡悟史; 押木守; 金田一智規; 新田見匡, 日本工業用水協会研究発表会講演要旨, 57th, 2022
• 比色分析と質量分析法を組み合わせた溶存無機態窒素の 15N/14N 比の新たな測定手法
  永井 孔明; 荒木 信夫; 鈴木 義之; 斉藤 信雄; 山口 隆司; 石井 聡; 押木 守, 第34回日本微生物生態学会年次大会, Oct. 2021
• なぜ低温硝化リアクター内で亜硝酸酸化細菌Nitrospira は優占できたのか～ Nitrospira とNitrosomonas の炭素固定経路の違い～
  根津 拓福; 黒田 恭平; 成廣 隆; 金田一 智規; 藤井 直樹; 荒木 信夫; 山口 隆司; 幡本 将史; 鈴木 義之; 岡部 聡; 押木 守, 第34回日本微生物生態学会年次大会, Oct. 2021
• 海洋性アナモックス細菌とAOAは相利共生するか？
  松本衿花; 森本衣美; 上薗亮達; Shaoyu YE; 小林香苗; 押木守; 岡部聡, 第34回日本微生物生態学会年次大会, Oct. 2021
• 海洋性アナモックス細菌‟Ca. Scalindua sp.”集積培養系における尿素及びシアン酸の代謝経路の解析
  森本衣美; 上薗亮達; 小林香苗; 押木守; 岡部聡, 第34回日本微生物生態学会年次大会, Oct. 2021
• Dual nitrogen and oxygen isotope fractionation during complete ammonia oxidation by comammox bacteria "Nitrospira inopinata"
  Baku Tsukada; Kanae Kobayashi; Keitaro Fukushima; Yuji Onishi; Mamoru Oshiki; Keisuke Koba; Satoshi Okabe, 7th International Conference on Nitrification and Related Processes, Jul. 2021
• Metabolic pathways of urea and cyanate in an enrichment culture of a marine anammox bacterium ‟Ca. Scalindua sp.”
  Morimoto, E; Kamizono, A; Kobayashi, K; Oshiki, M; Okabe, S, 7th International Conference on Nitrification and Related Processes, Jul. 2021
• Colorimetric MALDI-TOF/MS; a simple and high-throughput method for determining 15N atom % of NH3, NO2-, NO3-, NH2OH and N2H4
  Mamoru Oshiki; Komei Nagai; Satoshi Ishii; Yoshiyuki Suzuki; Nobuo Saito; Takashi Yamaguchi; Nobuo Araki; Satoshi Okabe, 7th International Conference on Nitrification and Related Processes, Jul. 2021
• Oxygen isotopic fractionation and exchange during anaerobic ammonium oxidation (anammox)
  Kanae Kobayashi; Keitaro Fukushima; Yuji Onishi; Kazuya Nishina; Akiko Makabe; Mamoru Oshiki; Keisuke Koba; Satoshi Okabe, 7th International Conference on Nitrification and Related Processes, Jul. 2021
• Mechanism for Suppression of Membrane Resistance using an SMP-degrading Bacteriophage
  渡部慶彦; 北島正章; 大坪千穂; 石崎創; 荒川千智; 石井聡; 押木守; 岡部聡, 日本水環境学会年会講演集, 55th, 2021
• Development of new nitrogen removal system by microalgal-bacteria consortia photo-baffled reactor
  福島巧己; 渡利高大; 幡本将史; 牧慎也; 山口隆司; 押木守; 川上周司, 日本水環境学会年会講演集, 54th, 2020
• Analysis of microbial community in UASB reactor during anaerobic sulfur oxidation reaction
  小林直央; 幡本将史; 牧慎也; 渡利高大; 山口隆司; 川上周司; 荒木信夫; 押木守, 日本水環境学会年会講演集, 54th, 2020
• Effect of endosymbiotic methanogen species on growth of anaerobic protists
  平片悠河; 幡本将史; 渡利高大; 山口隆司; 荒木信夫; 押木守, 日本水環境学会年会講演集, 54th, 2020
• 嫌気性原生動物とメタン生成古細菌の細胞内共生関係を利用したメタン生成古細菌の分離・培養への試み
  阿久澤秀磨; 平片悠河; 渡利高大; 幡本将史; 山口隆司; 荒木信夫; 押木守, 土木学会年次学術講演会講演概要集(CD-ROM), 75th, 2020
• Analysis of nitrogen and oxygen isotope effects of a marine anammox species; “Ca. Scalindua sp.”
  小林香苗; 福島慶太郎; 大西雄二; 仁科一哉; 眞壁明子; 押木守; 金田一智規; 木庭啓介; 岡部聡, 日本水環境学会年会講演集, 54th, 2020
• Acquisition of endosymbiotic methanogen from the natural environment by Cyclidium. sp.
  阿久澤秀磨; 平片悠河; 渡利高大; 幡本将史; 山口隆司; 荒木信夫; 押木守, 日本水環境学会年会講演集, 54th, 2020
• UASBスラッジベッド内での原生動物のサバイバル
  佐藤剛; 荒木信夫; 押木守; 山口隆司; 平片悠河, 日本水環境学会年会講演集, 53rd, 2019
• 嫌気性原生動物Cyclidium sp.細胞内に共生可能なメタン生成古細菌の種の特定
  平片悠河; 幡本将史; 渡利高大; 山口隆司; 押木守; 荒木信夫; 延優, 日本微生物生態学会大会(Web), 33rd, 2019
• UASBリアクターにおける硫黄酸化遺伝子に着目した微生物解析
  小林直央; 川上周司; 幡本将史; 牧慎也; 渡利高大; 惣中英章; 山口隆司; 荒木信夫; 押木守, 日本水環境学会年会講演集, 53rd, 2019
• UASB槽内から分離した嫌気性原生動物の捕食特性と基質となる細菌が増殖に及ぼす影響
  平片悠河; 幡本将史; 渡利高大; 山口隆司; 押木守; 荒木信夫, 日本水環境学会年会講演集, 53rd, 2019
• 嫌気性原生動物Cyclidium sp.とメタン生成古細菌の細胞内共生における種特異性
  平片悠河; 渡利高大; 幡本将史; 山口隆司; 押木守; 荒木信夫, 土木学会年次学術講演会講演概要集(CD-ROM), 74th, 2019
• 硫黄酸化還元に関与する機能遺伝子を標的とした嫌気的硫黄酸化反応に関与する微生物の解析
  小林直央; 幡本将史; 牧慎也; 渡利高大; 惣中英章; 山口隆司; 川上周司; 荒木信夫; 押木守, 土木学会年次学術講演会講演概要集(CD-ROM), 74th, 2019
• 嫌気性アンモニア酸化細菌“Ca.Scalindua sp.”の酸素同位体分別の解析
  小林香苗; 福島慶太郎; 大西雄二; 眞壁明子; 矢野翠; 押木守; 金田一智規; 木庭啓介; 岡部聡, 日本微生物生態学会大会(Web), 33rd, 2019
• 製鉄排水処理プロセスの微生物群集解析~ビッグデータ解析への挑戦~
  福島寿和; 中川淳一; 川野秀一; 押木守, 日本水環境学会シンポジウム講演集, 21st, 189, 04 Sep. 2018
  Japanese
• アンモニア酸化古細菌Nitrososphaera viennensisの亜硝酸還元酵素の異種発現および機能解析
  押木守; 小林駿; 荒木信夫; 平大輔; 吉田圭太朗; 豊福雅典; 志田洋介; 小笠原渉; 山口隆司, 日本水環境学会シンポジウム講演集, 21st, 181‐182, 04 Sep. 2018
  Japanese
• 都市下水処理UASB槽内に生息する原生動物Cyclidium sp.の細菌捕食速度と代謝物の調査
  平片悠河; 渡利高大; 幡本将史; 山口隆司; 押木守; 荒木信夫, 土木学会年次学術講演会講演概要集(CD-ROM), 73rd, ROMBUNNO.VII‐101, 01 Aug. 2018
  Japanese
• 嫌気性アンモニウム酸化細菌を用いたヒドラジン生産型窒素含有排水処理法の開発
  押木守, 建設工学研究振興会年報, 53, 29‐32, 27 Jul. 2018
  Japanese
• nos欠損型脱窒菌による窒素含有廃水の処理及びN₂O回収による新エネルギー源の創出
  石丸美穂; 押木守; 荒木信夫; 岡部聡; 幡本将史; 山口隆司, 日本水環境学会年会講演集, 52nd, 656, 09 Mar. 2018
  Japanese
• 都市下水処理UASBスラッジベッド内の嫌気性原生動物の棲かについて
  佐藤剛; 荒木信夫; 押木守; 吉田力; 山口隆司; 平片悠河; 難波悠太, 日本水環境学会年会講演集, 52nd, 657, 09 Mar. 2018
  Japanese
• 窒素安定同位体比を用いた嫌気性原生動物の食物環の解明
  桑原大輝; 荒木信夫; 押木守; 小野寺崇; 高津文人; 珠坪一晃; 山口隆司, 日本水環境学会年会講演集, 52nd, 658, 09 Mar. 2018
  Japanese
• アナモックス細菌の窒素・酸素同位体効果の解析
  小林香苗; 眞壁明子; 押木守; 金田一智規; 岡部聡, 日本水環境学会年会講演集, 52nd, 379, 09 Mar. 2018
  Japanese
• DHSリアクターを用いた低温海水魚水槽での硝化技術の開発
  相塚陸; SHER Angelin TAN YONG; 荒木信夫; 押木守; 山口隆司; 大森聖史; 長野晃弘, 日本水環境学会年会講演集, 52nd, 659, 09 Mar. 2018
  Japanese
• 初沈+DHS下水処理システムにおける安全な処理水の再利用を実現する消毒システムの開発
  景政柊蘭; 長町晃宏; 多川正; 井口晃徳; 久保田健吾; 原田秀樹; 押木守; 荒木信夫; 大久保努; 上村繁樹, 日本水環境学会年会講演集, 52nd, 144, 09 Mar. 2018
  Japanese
• 都市下水処理エアレーションタンク内で好気性脱窒反応を行う細菌の特定
  樋口裕武; 荒木信夫; 押木守; ANGELIN; 山口隆司; 青木仁孝, 日本水環境学会年会講演集, 52nd, 660, 09 Mar. 2018
  Japanese
• 都市下水処理UASB槽内に生息する原生動物の細菌の捕食と代謝特性の解明
  平片悠河; 幡本将史; 渡利高大; 山口隆司; 押木守; 荒木信夫, 日本水環境学会年会講演集, 52nd, 354, 09 Mar. 2018
  Japanese
• 海洋性アナモックス細菌の集積培養装置の作製および異なる塩分濃度下における脱窒能の比較
  田中孝国; フー レイチア; 押木守; 金田一智規; 新田見匡, 日本工業用水協会研究発表会講演要旨, 53rd, 76‐78, 2018
  Japanese
• 都市下水を処理するUASB槽内に生息する原生動物群集の季節変動と処理水質との関連
  平片悠河; 幡本将史; 山口隆司; 押木守; 荒木信夫, 日本水環境学会シンポジウム講演集, 20th, 29‐30, 26 Sep. 2017
  Japanese
• 18S rRNA遺伝子情報を用いた都市下水を処理するUASB槽及び活性汚泥における原生動物群集解析
  平片悠河; 幡本将史; 山口隆司; 押木守; 荒木信夫, 土木学会年次学術講演会講演概要集(CD-ROM), 72nd, ROMBUNNO.VII‐187, 01 Aug. 2017
  Japanese
• 国内下水処理場におけるバルキング関連糸状性Kouleothrix属細菌の調査
  新田見匡; 小柴佑介; 福田淳二; 庄司仁; 野口愛; 押木守; 黒田真史; 金田一智規; 栗栖太, 下水道研究発表会講演集, 54th, 946‐948, 03 Jul. 2017
  Japanese
• 初沈+DHSシステムにおける処理水質の長期安定性に関する検証
  長町晃宏; 景政柊蘭; 多川正; 井口晃徳; 押木守; 荒木信夫; 大久保努; 上村繁樹; 久保田健吾; 高橋優信; 原田秀樹, 日本水環境学会年会講演集, 51st, 438, 09 Mar. 2017
  Japanese
• 上昇線流速がUASB槽内の嫌気性原生動物に及ぼす影響
  吉田力; 難波悠太; 押木守; 荒木信夫; 平片悠河; 幡本将史; 山口隆司, 日本水環境学会年会講演集, 51st, 637, 09 Mar. 2017
  Japanese
• 下水処理DHSリアクターの運転条件がウイルス除去性能に及ぼす影響
  井口晃徳; 久保田健吾; 稲葉愛美; 原田秀樹; 大久保努; 上村繁樹; 長町晃宏; 多川正; 押木守; 荒木信夫; 瀬戸雄太; 幡本将史; 山口隆司; 高橋優信, 日本水環境学会年会講演集, 51st, 440, 09 Mar. 2017
  Japanese
• 乾燥途上国における下水の灌漑再利用のための処理システムの開発
  景政柊蘭; 長町晃宏; 多川正; 久保田健吾; 原田秀樹; 井口晃徳; 押木守; 荒木信夫; 大久保努; 上村繁樹, 日本水環境学会年会講演集, 51st, 648, 09 Mar. 2017
  Japanese
• 都市下水処理エアレーションタンク内の脱窒素細菌叢の解析
  高橋晃平; 押木守; 荒木信夫; 平片悠河; 幡本将史; 山口隆司, 日本水環境学会年会講演集, 51st, 357, 09 Mar. 2017
  Japanese
• 下水灌漑におけるウイルスを対象とした健康リスク評価
  田中周弥; 大久保努; 上村繁樹; 多川正; 井口晃徳; 押木守; 荒木信夫; 高橋優信; 久保田健吾; 原田秀樹, 日本水環境学会年会講演集, 51st, 632, 09 Mar. 2017
  Japanese
• 18S rRNA遺伝子に基づく都市下水処理UASB槽内の原生動物群集の解析
  平片悠河; 幡本将史; 山口隆司; 押木守; 荒木信夫, 日本水環境学会年会講演集, 51st, 341, 09 Mar. 2017
  Japanese
• ナノ流路デバイスを用いた下水中RNAウイルス定量およびタイピング:DHSリアクターへの適用例
  押木守; 小林直央; 荒木信夫; 伊藤寿宏; 佐野大輔; 三浦尚之; 風間しのぶ; 原田秀樹; 久保田健吾; 幡本将史; 山口隆司; 井口晃徳; 瀬川高弘; 加藤毅; 多川正; 大久保努; 上村繁樹; 高橋優信, 日本水環境学会年会講演集, 51st, 546, 09 Mar. 2017
  Japanese
• 鉄還元型嫌気性アンモニア酸化プロセスによる超省エネルギーかつ省コストな窒素除去技術の創出
  押木守; 荒木信夫; 川上周司; 佐藤久, 環境助成研究成果 概要集 第35回・2014年度分:5件 第36回・2015年度分:28件 平成29年, 25‐26, 2017
  Japanese
• 都市下水処理UASB槽内に生息する原生動物群集解析と分離培養の試み
  平片悠河; 幡本将史; 山口隆司; 押木守; 荒木信夫, 日本微生物生態学会大会(Web), 2017, ROMBUNNO.P‐361 (WEB ONLY), 2017
  Japanese
• ヒドラジンを合成するオルガネラ:嫌気性アンモニウム酸化細菌のアナモキソソーム
  押木守; 岡部聡, 日本微生物生態学会大会(Web), 2017, ROMBUNNO.S‐070 (WEB ONLY), 2017
  Japanese
• 国内下水処理場におけるバルキング関連糸状性Kouleothrix属細菌の調査 (第54回下水道研究発表会講演集)
  新田見 匡; 小柴 佑介; 福田 淳二; 庄司 仁; 野口 愛; 押木 守; 黒田 真史; 金田 一; 栗栖 太, 下水道研究発表会講演集, 54, 946, 948, 2017
  日本下水道協会, Japanese
• FISH法と定量PCR法による国内下水処理施設におけるバルキング関連糸状性細菌の探索
  新田見匡; 庄司仁; 野口愛; 押木守; 黒田真史; 金田一智規; 福田淳二; 栗栖太, 日本生物工学会大会講演要旨集, 68th, 305, 25 Aug. 2016
  Japanese
• ラボスケールUASBリアクター内における嫌気的硫黄酸化反応の再現性評価
  塩澤大和; THUY Tran; Thi Thanh; 牧慎也; 幡本将史; 山口隆司; 山崎真一; 押木守; 荒木信夫, 土木学会年次学術講演会講演概要集(CD-ROM), 71st, ROMBUNNO.VII‐108, 01 Aug. 2016
  Japanese
• 下水処理UASB槽内に生息する嫌気性原生動物の共生微生物の分子生物学的解析
  平片悠河; 幡本将史; 山口隆司; 押木守; 荒木信夫, 土木学会年次学術講演会講演概要集(CD-ROM), 71st, ROMBUNNO.VII‐107, 01 Aug. 2016
  Japanese
• 都市下水処理を目的とした一次沈殿槽+DHSリアクターを用いた処理性能評価
  長町晃宏; 景政柊蘭; 野村妃奈; 多川正; 井口晃徳; 押木守; 荒木信夫; 大久保努; 上村繁樹; 久保田健吾; 高橋優信; 原田秀樹, 土木学会年次学術講演会講演概要集(CD-ROM), 71st, ROMBUNNO.VII‐097, 01 Aug. 2016
  Japanese
• キレイな水の作り方教えます 〜水環境問題と水浄化技術〜
  荒木信夫; 押木守, ひらめきときめきサイエンス, Jul. 2016
• 淡水・海水生物飼育水におけるUSB‐DHSシステムを用いた窒素成分除去性能の評価
  太田優輝; NUR Adlin; 渡邉高子; 牧慎也; 幡本将史; 山口隆司; 押木守; 荒木信夫; 青柳彰, 日本水環境学会年会講演集, 50th, 144, 10 Mar. 2016
  Japanese
• 活性汚泥の沈降性に対するKouleothrix属糸状性細菌の影響―日本各地の下水処理施設における実態調査―
  庄司仁; 新田見匡; 金田一智規; 黒田真史; 野口愛; 押木守, 日本水環境学会年会講演集, 50th, 227, 10 Mar. 2016
  Japanese
• 気候条件を考慮した下水の灌漑利用に対する健康影響評価
  内田翔太; 上村繁樹; 大久保努; 荒木信夫; 押木守; 多川正; 井口晃徳; 高橋優信; 久保田健吾; 原田秀樹, 日本水環境学会年会講演集, 50th, 277, 10 Mar. 2016
  Japanese
• Anammox細菌の窒素同位体分別に関する研究
  小林香苗; 押木守; 金田一智規; 眞壁明子; 岡部聡, 日本水環境学会年会講演集, 50th, 664, 10 Mar. 2016
  Japanese
• DHS(downflow hanging sponge)リアクターにおける病原ウイルスの除去効率
  小林直央; 押木守; 荒木信夫; 本山毅宜; 伊藤寿宏; 佐野大輔; 瀬川高弘; 幡本将史; 山口隆司; 井口晃徳; 上村繁樹; 大久保努; 多川正; 高橋優信; 久保田健吾; 原田秀樹, 日本水環境学会年会講演集, 50th, 657, 10 Mar. 2016
  Japanese
• Real‐time PCR法によるバルキング関連Kouleothrix属糸状性細菌の定量
  新田見匡; 福田淳二; 押木守; 栗栖太, 日本水環境学会年会講演集, 50th, 385, 10 Mar. 2016
  Japanese
• 一次沈殿槽とDHSリアクターを組み合わせた無曝気方式の新規下水処理技術の処理性能評価
  長町晃宏; 景政柊蘭; 野村妃奈; 多川正; 井口晃徳; 押木守; 荒木信夫; 大久保努; 上村繁樹; 久保田健吾; 高橋優信; 原田秀樹, 日本水環境学会年会講演集, 50th, 658, 10 Mar. 2016
  Japanese
• 脱塩素化細菌Geobacter sp.AY株が保有する脱塩素酵素の機能解析
  小林直央; 押木守; 吉田奈央子; 幡本将史; 山口隆司; 荒木信夫, 日本微生物生態学会大会(Web), 31st, 215 (WEB ONLY), 2016
  Japanese
• アンモニア酸化古細菌Nitrososphaera viennensis由来の銅含有型亜硝酸還元酵素NirKの異種発現および酵素学的解析
  小林駿; 押木守; 志田洋介; 幡本将史; 山口隆司; 小笠原渉; 荒木信夫, 日本微生物生態学会大会(Web), 31st, 214 (WEB ONLY), 2016
  Japanese
• 嫌気性アンモニウム酸化細菌と共に : これまでの研究生活を振り返って(扉を拓く)
  押木 守, 日本微生物生態学会誌, 31, 1, 9, 11, 2016
  日本微生物生態学会, Japanese
• 都市下水処理UASB槽の処理性能および微生物叢に及ぼす嫌気性原生動物の影響
  平片悠河; 黒田恭平; 幡本将史; 山口隆司; 押木守; 荒木信夫, 日本水環境学会シンポジウム講演集, 18th, 181, 182, 14 Sep. 2015
  Japanese
• 培地の酸化還元電位が嫌気性原生動物の優占種に及ぼす影響
  石塚充朗; 平方悠河; 荒木信夫; 押木守; 山口隆司; 幡本将史, 日本水環境学会年会講演集, 49th, 60, 16 Mar. 2015
  Japanese
• 嫌気性アンモニウム酸化細菌のnirS遺伝子の機能解析
  小林駿; 押木守; 蝶名林郁也; 荒木信夫; 吉田圭太郎; 豊福雅典, 日本微生物生態学会大会(Web), 30th, PD‐061(JTK) (WEB ONLY), 2015
  Japanese
• 嫌気性アンモニウム酸化細菌由来ヒドラジン合成酵素の精製
  押木守; 荒木信夫; 岡部聡, 日本微生物生態学会大会(Web), 30th, PD‐051 (WEB ONLY), 2015
  Japanese
• Gemmatimonas aurantiacaによる亜酸化窒素(N₂O)の還元
  外山結加; 押木守; 荒木信夫, 日本微生物生態学会大会(Web), 30th, PD‐052 (WEB ONLY), 2015
  Japanese
• ゲノミクス,プロテオミクス解析から読み解くアンモニア酸化(anammox)細菌“Ca.Scalindua japonica”の生活環
  押木守; 水戸佳祐; 木村善一郎; 金田一智規; 佐藤久; 岡部聡, 日本ゲノム微生物学会年会要旨集, 8th, 69, 2014
  Japanese
• 嫌気性原生動物細胞内に共生する難培養性バクテリア・アーキアの分子生物学的解析
  平片悠河; 押木守; 荒木信夫; 黒田恭平; 幡本将史; 山口隆司, 日本土壌微生物学会講演要旨集, 2014, 146, 146, 2014
  日本微生物生態学会, Japanese
• P13-17 嫌気性原生動物細胞内に共生する難培養性バクテリア・アーキアの分子生物学的解析(ポスター発表)
  平片 悠河; 押木 守; 荒木 信夫; 黒田 恭平; 幡本 将史; 山口 隆司, 日本微生物生態学会講演要旨集, 2014, 146, 146, 2014
  日本微生物生態学会, Japanese
• 嫌気性アンモニア酸化(anammox)細菌の代謝多様性と廃水処理への応用
  押木守; 佐藤久; 岡部聡, 日本生物工学会大会講演要旨集, 65th, 89, 25 Aug. 2013
  Japanese
• 2S-Cp04 Physiological and genomic insights into an anammox bacterium "Candidatus Brocadia sinica"
  Oshiki Mamoru; Satoh Hisashi; Okabe Satoshi, 日本生物工学会大会講演要旨集, 65, 89, 89, 25 Aug. 2013
  公益社団法人日本生物工学会, Japanese
• Physiological characterization of an anaerobic ammonium-oxidizing bacterium enriched from coastal sediments,Hiroshima,Japan
  Takanori Awata; Mamoru Oshiki; Tomonori Kindaichi; Noriatsu Ozaki; Akiyoshi Ohashi; Satoshi Okabe, The 2nd International Anammox Symposium, pp.67, Jun. 2013, [Peer-reviewed]
  The effect of salinity on organic removal and ammonium oxidation in a down-flow hanging sponge reactor was investigated by conducting a long-term continuous experiment over a period of 800 days. The DHS reactor, constructed by connecting three identical units, was fed with artificial wastewater containing 500 mg-N/L of ammonium nitrogen and 1400 mg-COD/L of phenol. Salinity of the influent was controlled by the addition of 8.0 to 25 g-Cl-/L of NaCl. The DHS reactor was operated at a hydraulic retention time of 12 h in a temperature controlled room at 25 degrees C. No significant inhibition of organic removal and ammonium oxidation was observed at salinities of up to 20 g-Cl-/L, at which levels ammonium oxidation and COD removal both exceeded 90%, respectively. However, at a salinity of 25 g-Cl-/L, organic removal and ammonium oxidation were both severely inhibited. In addition, the ratio of effluent nitrite nitrogen to influent ammonium nitrogen increased from 3.4% at salinities of 8.0 g-Cl-/L to 33% at salinities of 20 g-Cl-/L., The 2nd International Anammox Symposium
• 広島湾により集積されたScalindua属アナモックス細菌の生理学的特性
  粟田貴宣; 金田一智規; 尾崎則篤; 大橋晶良; 押木守; 岡部聡, 日本水環境学会年会講演集, 47th, 160, 11 Mar. 2013
  Japanese
• 環境サンプル測定のためのリン酸マイクロセンサーの開発
  宮崎悠爾; 谷内翔; 押木守; 佐藤久; 高橋正宏; 岡部聡, 土木学会北海道支部論文報告集(CD-ROM), 69, ROMBUNNO.G-02, Feb. 2013
  Japanese
• 環境サンプル測定のためのリン酸マイクロセンサーの開発
  宮崎悠爾; 谷内翔; 押木守; 高橋正宏; 岡部聡; 佐藤久, 衛生工学シンポジウム論文集, 20th, 24, 04 Dec. 2012
  Japanese
• 環境サンプル測定のためのリン酸マイクロセンサーの開発
  宮崎悠爾; 谷内翔; 押木守; 佐藤久; 高橋正宏; 岡部聡, 環境工学研究フォーラム講演集, 49th, 25, 27, 28 Nov. 2012
  Japanese
• PGa-04 Elucidating a metabolic pathway for the anaerobic ammonium oxidation of Candidatus "Brocadia sinica" by metagenomics analysis
  Oshiki Mamoru; Shinyako Kaori; Satoh Hisashi; Okabe Satoshi, 日本微生物生態学会講演要旨集, 28, 160, 160, 19 Sep. 2012
  日本微生物生態学会, English
• 嫌気性アンモニア酸化(アナモックス)細菌のゲノム解析が明らかにする生理学的特性
  押木守; 佐藤久; 岡部聡; 新家子香織, 日本水環境学会シンポジウム講演集, 15th, 157, 10 Sep. 2012
  Japanese
• Physiological characteristics of marine anammox bacteria enriched from sea sediments,Hiroshima,Japan.
  Takanori Awata; Tomonori Kindaichi; Noriatsu Ozaki; Akiyoshi Ohashi; Mamoru Oshiki; Satoshi Okabe, 14th International Symposium on Microbial Ecology(isme14), PS09.457A, PS09.457A, 19 Aug. 2012, [Peer-reviewed]
  14th International Symposium on Microbial Ecology(isme14)
• Anammox菌による鉄酸化硝酸還元能の解析
  石黒真規; 押木守; 石井聡; 岡部聡, 日本水環境学会年会講演集, 46th, 151, 14 Mar. 2012
  Japanese
• Anammox活性の菌体密度依存的制御機構の解明
  平泉晴菜; 押木守; 岡部聡, 日本水環境学会年会講演集, 46th, 152, 14 Mar. 2012
  Japanese
• 難培養性微生物の生理学的特性が知りたいっ
  押木守, 日本微生物生態学会誌, 27, 1, 8, 9, 01 Mar. 2012
  日本微生物生態学会, Japanese
• 難培養性微生物の生理学的特性が知りたいっ(優秀賞,リサーチ最前線)
  押木 守, 日本微生物生態学会誌, 27, 1, 8, 9, 01 Mar. 2012
  日本微生物生態学会, Japanese
• 環境サンプル測定のためのリン酸マイクロセンサーの開発
  谷内翔; 宮崎悠爾; 押木守; 佐藤久; 高橋正宏; 岡部聡, 環境工学研究フォーラム講演集, 48th, 217, 219, 25 Nov. 2011
  Japanese
• メタゲノム・プロテオームアプローチによる嫌気性アンモニア酸化(anammox)細菌Candidatus‘Brocadia sinica’の生理生態学的特性の解明
  押木守; 新家子香織; 佐藤久; 岡部聡, 日本微生物生態学会講演要旨集, 27th, 27, 72, 72, 08 Oct. 2011
  日本微生物生態学会, Japanese
• Anammox細菌Candidatus“Brocadia sinica”の嫌気性アンモニア酸化活性に及ぼす菌体密度の影響
  平泉晴菜; 新家子香織; 押木守; 佐藤久; 岡部聡, 日本微生物生態学会講演要旨集, 27th, 27, 73, 73, 08 Oct. 2011
  日本微生物生態学会, Japanese
• PA-034 Anammox細菌Candidatus "Brocadia sinica"の嫌気性アンモニア酸化活性に及ぼす菌体密度の影響(PA 水処理生態系,ポスター発表)
  平泉 晴菜; 新家子 香織; 押木 守; 佐藤 久; 岡部 聡, 日本微生物生態学会講演要旨集, 27, 73, 73, 08 Oct. 2011
  日本微生物生態学会, Japanese
• PA-030 メタゲノム・プロテオームアプローチによる嫌気性アンモニア酸化(anammox)細菌Candidatus 'Brocadia sinica'の生理生態学的特性の解明(PA 水処理生態系,ポスター発表)
  押木 守; 新家子 香織; 佐藤 久; 岡部 聡, 日本微生物生態学会講演要旨集, 27, 72, 72, 08 Oct. 2011
  日本微生物生態学会, Japanese
• 部分硝化‐AnammoxプロセスからのN₂O生成
  岡部聡; 押木守; 高橋慶考; 佐藤久, 日本水環境学会シンポジウム講演集, 14th, 43, 44, 10 Sep. 2011
  Japanese
• 環境サンプル測定のためのリン酸マイクロセンサーの開発
  宮崎悠爾; 押木守; 高橋正宏; 岡部聡; 佐藤久, 土木学会年次学術講演会講演概要集(CD-ROM), 66th, ROMBUNNO.VII-029, 05 Aug. 2011
  Japanese
• Development of an Aerobic Granule Reactor for Partial Nitrification Combined with Anammox
  SONG Yanjun; 押木守; 岡部聡, 日本水環境学会年会講演集, 45th, 161, 18 Mar. 2011
  Japanese
• Anammox細菌の代謝活性に及ぼす菌体密度の影響
  平泉晴菜; 新家子香織; 押木守; 岡部聡, 日本水環境学会年会講演集, 45th, 47, 18 Mar. 2011
  Japanese
• 新規anammox細菌Candidatus ‘Brocadia sinica’における網羅的プロテオーム解析
  新家子香織; 押木守; 佐藤久; 岡部聡, 日本水環境学会年会講演集, 45th, 46, 18 Mar. 2011
  Japanese
• P-46 Metagenomic analyses for a novel anammox bacterium, Candidatus "Brocadia gluttonica", responsible for high-rate nitrogen removal(Poster Session)
  SHINYAKO KAORI; OSHIKI MAMORU; SATOU HISASHI; OKABE SATOSHI, 日本微生物生態学会講演要旨集, 26, 117, 117, 23 Nov. 2010
  日本微生物生態学会, English
• 環境サンプル測定のためのリン酸マイクロセンサーの開発
  宮崎悠爾; 押木守; 佐藤久; 高橋正宏; 岡部聡, 環境工学研究フォーラム講演集, 47th, 4, 6, 12 Nov. 2010
  Japanese
• 余剰汚泥消化プロセスにおけるポリヒドロキシアルカン酸(PHA)の分解挙動
  押木守; 佐藤弘泰; 味埜俊, 日本水環境学会年会講演集, 44th, 72, 15 Mar. 2010
  Japanese
• 超音波破砕・希釈法によるDNA抽出のバイアスの評価
  高梨正訓; 押木守; 小貫元治; 佐藤弘泰; 味埜俊, 環境工学研究フォーラム講演集, 46th, 170, 172, 27 Nov. 2009
  Japanese
• 1A-8(P-75) 超音波破砕・希釈によるPCR反応用鋳型試料調製法の各種試料への適用(口頭発表+ポスター発表)
  佐藤 弘泰; 押木 守; 近藤 宗俊; WASANA GUNAWARDANA; 高梨 正訓; 鯉渕 幸生; 味埜 俊, 日本微生物生態学会講演要旨集, 25, 4, 4, 21 Nov. 2009
  日本微生物生態学会, Japanese
• 微生物のもつ有機物貯蔵能力の有価物回収への利用
  佐藤弘泰; 押木守; 小貫元治; 味埜俊, 日本水環境学会シンポジウム講演集, 12th, 164, 165, 14 Sep. 2009
  Japanese
• 活性汚泥中細菌による生分解性プラスチックPHAの生産~菌体外に見いだされたPHA顆粒~
  押木守; 小貫元治; 佐藤弘泰; 味埜俊, 日本水環境学会シンポジウム講演集, 12th, 166, 167, 14 Sep. 2009
  Japanese
• 微生物のもつ有機物貯蔵能力を利用した省エネルギー型排水処理法の提案
  押木守; 佐藤弘泰; 味埜俊, 用水と廃水, 51, 9, 737, 745, 01 Sep. 2009
  産業用水調査会, Japanese
• Aeration-saving and biomass recovery treatment through temporal carbon storage and adsorption process by activated sludge
  押木 守; 佐藤 弘泰; 味埜 俊, Journal of water and waste, 51, 9, 737, 745, Sep. 2009
  産業用水調査会, Japanese
• 活性汚泥内に存在する細胞外PHA顆粒の観察
  押木守; 佐藤弘泰; 味埜俊, 衛生工学シンポジウム論文集, 17th, 18, 29 Jun. 2009
  Japanese
• 一時貯蔵反応を利用した省曝気・有機物回収型廃水処理プロセスの提案
  押木守; 小貫元治; 佐藤弘泰; 味埜俊, 日本水環境学会年会講演集, 43rd, 54, 16 Mar. 2009
  Japanese
• 超音波破砕・希釈によるPCR反応用鋳型試料調製法の各種試料への適用
  佐藤弘泰; 押木守; 近藤宗俊; GUNAWARDANA Wasana; 高梨正訓; 鯉渕幸生; 味埜俊, 日本微生物生態学会講演要旨集, 25th, 25, YOSHI4, 4, 2009
  日本微生物生態学会, Japanese
• 活性汚泥による有機物除去へのPHA蓄積細菌の寄与
  押木守; 佐藤弘泰; 味埜俊; 小貫元治, 日本水環境学会シンポジウム講演集, 11th, 201, 206, 17 Sep. 2008
  Japanese
• 有機物除去過程における有機性一時貯蔵物質の生成
  押木守; 佐藤弘泰; 味埜俊; 小貫元治, 下水道研究発表会講演集, 45th, 785, 787, 30 Jun. 2008
  Japanese
• 有機物除去過程における有機性一時貯蔵物質の生成 (第45回下水道研究発表会講演集)
  押木 守; 佐藤 弘泰; 味埜 俊, 下水道研究発表会講演集, 45, 785, 787, 2008
  日本下水道協会, Japanese
• 活性汚泥による好気条件下での有機物除去過程における一時貯蔵物質
  押木守; 小貫元治; 佐藤弘泰; 味埜俊, 環境工学研究フォーラム講演集, 44th, 78, 80, 16 Nov. 2007
  Japanese
• 3-19 Microbial composition of PHA (polyhydroxyalkanoate)-accumulating organisms in activated sludge and its distribution in eight full-scale wastewater treatment plants(Waste water treatment ecosystem,Oral presentation) :
  Oshiki Mamoru; Onuki Motoharu; Satoh Hiroyasu; Mino Takashi, 日本微生物生態学会講演要旨集, 23, 72, 72, 2007
  日本微生物生態学会, English
• B-19 Application of buoyant density separation method and fluorescence in-situ hybridization for the identification of PHA-accumulating organisms in full-scale wastewater treatment plants(Water/Wastewater treatment ecosystem,(2)Oral presentation)
  Oshiki Mamoru; Onuki Motoharu; Satoh Hiroyasu; Mino Takashi, 日本微生物生態学会講演要旨集, 22, 251, 251, 27 Oct. 2006
  日本微生物生態学会, Japanese
• PA-39 Phylogenetic analysis of polyphosphate accumulating bacteria isolated by micromanipulation from enhanced biological phosphorus removal sludge(WATER-TREATMENT ECOSYSTEM,Session A,(1) Poster presentations)
  Okunuki Suguru; Oshiki Mamoru; Kawaharasaki Mamoru; Kanagawa Takahiro; Tanaka Hideo, 日本微生物生態学会講演要旨集, 21, 85, 85, 30 Oct. 2005
  日本微生物生態学会, Japanese
• B-22 Screening of Polyhydroxyalkanoate(PHA)-Accumulating Bacteria in Activated Sludge by Micromanipulation(WATER-TREATMENT ECOSYSTEM,(2) Oral presentation)
  Oshiki Mamoru; Okunuki Suguru; Onuki Motoharu; Satoh Hiroyasu; Mino Takashi; Kawaharasaki Mamoru; Kanagawa Takahiro, 日本微生物生態学会講演要旨集, 21, 242, 242, 30 Oct. 2005
  日本微生物生態学会, Japanese
• 生物学的リン除去汚泥からマイクロマニピュレーションにより分離されたポリリン酸蓄積細菌の系統及びin situ解析
  奥貫優; 押木守; 川原崎守; 金川貴博; 田中秀夫, 日本生物工学会大会講演要旨集, 2005, 78, 78, 25 Sep. 2005
  日本生物工学会, Japanese
• 1B14-3 Phylogenetic and in situ analysis of polyphosphate accumulating bacteria isolated by micromanipulation from enhanced biological phosphorus removal sludge
  OKUNUKI Suguru; OSIHIKI Mamoru; KAWAHARASAKI Mamoru; KANAGAWA Takahiro; TANAKA Hideo, 日本生物工学会大会講演要旨集, 17, 78, 78, 25 Sep. 2005
  公益社団法人日本生物工学会, Japanese
• シーケンス爆気運転による溶存酸素濃度ストレスが硝化,脱窒素細菌の機能遺伝子の転写に及ぼす影響の解析
  押木守; 荒木信夫; 長野晃弘; 山口隆司; 山崎慎一, 日本水環境学会年会講演集, 38th, 244, 17 Mar. 2004
  Japanese
• 28-B-07 活性汚泥内におけるアンモニア酸化細菌のamoA mRNA転写量とアンモニア酸化速度の関係(水処理生態系,一般講演)
  荒木 信夫; 押木 守; 山口 隆司; 山崎 慎一, 日本微生物生態学会講演要旨集, 19, 102, 102, 26 Oct. 2003
  日本微生物生態学会, Japanese
• アンモニア酸化細菌のamoA mRNAダイナミクスに関する研究
  押木守; 竹林賢; 荒木信夫; 山口隆司; 山崎慎一, 日本水環境学会年会講演集, 37th, 430, 04 Mar. 2003
  Japanese

• 微生物集団の機能解明に向けた統計的アプローチ:複雑系データ解析の新展開
  押木 守; 福島 寿和; 川野 秀一; 中川 淳一
  日本分析化学会第74年会、口頭発表 C1104S、北海道大学工学部, 24 Sep. 2025
  24 Sep. 2025 - 26 Sep. 2025, [Invited]
• Transmission of antibiotic-resistance plasmids in activated sludge
  Mamoru Oshiki; Kosuke Higuchi; Kiko Ohara; Satoshi Okabe
  The 10th International Water Industry Conference (IWIC2024), EXCO, Daegu, Korea, 12 Nov. 2024, Invited oral presentation
  12 Nov. 2024 - 15 Nov. 2024, [Invited]
• 亜硝酸還元酵素による亜酸化窒素(N2O)の生成
  押木 守; 小林 諒斗; 中屋 佑紀; 服部 祥平; 豊田 栄; 佐藤 久; 岡部 聡
  日本微生物生態学会 第36回大会 (11月27日〜11月30日、浜松アクトシティ) 3_31_S-a02 2023年11月, 30 Nov. 2023, Invited oral presentation
  28 Nov. 2023 - 30 Nov. 2023, [Invited]
• 生物学的廃水処理プロセスにおける薬剤耐性プラスミドの動態
  押木 守; 樋口 宏介; 小原 紀子; 岡部 聡
  日本微生物生態学会 第36回大会 (11月27日〜11月30日、浜松アクトシティ) 1_31_S-p05 2023年11月, 28 Nov. 2023, Invited oral presentation
  28 Nov. 2023 - 30 Nov. 2023, [Invited]
• 下廃水処理に関わる 微生物群とその生理について
  押木守
  生物工学会北日本支部シンポジウム, 17 Dec. 2022, Invited oral presentation
  [Invited]
• Insights into the microbial ecology of nitrifying bacteria in marine recirculating trickling biofilter reactors
  Mamoru Oshiki; Hirotoshi Netsu; Kyohei Kuroda; Takashi Narihiro; Naoki Fujii; Tomonori Kindaichi; Yoshiyuki Suzuki; Takashiro Watari; Masashi Hatamoto; Takashi Yamaguchi; Nobuo Araki; Satoshi Okabe
  12th Asian Symposium on Microbial Ecology, 19 Apr. 2022
  [Invited]
• 嫌気性アンモニウム酸化(anammox)細菌クロニクル; 未知の代謝を解明する
  押木守
  第四回北関東磐越地区化学技術フォーラム, 2018
  郡山地域職業訓練センター, [Invited]
• Anaerobic ammonium oxidation (anammox) by an anammox bacterium "Candidatus Brocadia sinica"
  Oshiki M
  The 8th Asian Symposium on Microbiology Ecology, 2016
  台北, [Invited]
• 未知転じて既知となる: 嫌気性アンモニア酸化細菌の発見がもたらした衝撃
  押木守
  48回GRL浜松セミナー, 2014
  静岡大学, [Invited]
• Nitrite reduction by anaerobic ammonium oxidation bacteria
  押木守
  平成25年度日本水環境学会北海道支部学術講演会, 2013
  北海道大学, [Invited]
• 嫌気性アンモニア酸化(Anammox)細菌の代謝多様性と廃水処理への応用
  押木守
  第65回日本生物工学会大会, 2013
  広島国際会議場, [Invited]
• 嫌気性アンモニア酸化(アナモックス)細菌のゲノム解析が明らかにする生理学的特性
  押木守
  第15回水環境学会シンポジウム, 2012
  佐賀大学, [Invited], [Domestic Conference]
• Aeration-saving and biomass recovery treatment through temporal carbon storage and absorption process by activated sludge
  Oshiki M
  International Workshop on Water and Wastewater Treatment, 2009
  台湾, [Invited], [International presentation]
• 活性汚泥による有機物除去へのPHA蓄積細菌の寄与
  第11回水環境学会シンポジウム, 2008
  関西大学, [Invited]

• 環境微生物工学特論, 2024年, 修士課程, 工学院
• 環境微生物工学特論, 2024年, 博士後期課程, 工学院
• 環境工学序論, 2024年, 学士課程, 工学部
• 物理化学要論, 2024年, 学士課程, 工学部
• 環境工学実験Ⅰ, 2024年, 学士課程, 工学部
• 環境工学実験Ⅱ, 2024年, 学士課程, 工学部

• Present
  日本微生物生態学会
• Present
  日本水環境学会
• Present
  土木学会
• Present
  日本生物工学会
• Present
  日本農芸化学会

• パーム油製造廃棄物を用いたBio-CNG製造のための発酵プロセスおよびCO₂選択吸着剤の開発
  日ASEAN科学技術・イノベーション協働連携事業(NEXUS)
  Jan. 2026 - Dec. 2028
  押木守; 川野秀一; Adeline Seak May Chua; Chong Yang Chuah
  科学技術振興機構(JST), 国際共同研究, Principal investigator, JPMJNX25F3
• 下廃水からの生物学的窒素除去に関する新たな経路の特定とその反応促進方法の開発
  科学研究費助成事業
  01 Apr. 2025 - 31 Mar. 2028
  荒木 信夫; 川上 周司; 多川 正; 押木 守; 渡利 高大
  日本学術振興会, 基盤研究(B), 香川高等専門学校, 25K01359
• 窒素固定のための生物ー非生物ハイブリッド光触媒系の創生
  科学研究費助成事業
  01 Apr. 2025 - 31 Mar. 2028
  王 謙; 押木 守
  日本学術振興会, 基盤研究(B), 名古屋大学, 25K01581
• 高温多湿な亜熱帯地域に適合するレトロフィット型高効率窒素除去プロセスの国際開発
  科学研究費助成事業
  09 Sep. 2024 - 31 Mar. 2028
  押木 守
  日本学術振興会, 国際共同研究加速基金(海外連携研究), 北海道大学, 24KK0196
• An ICT-driven mobile and portable platform for greenhouse gases monitoring in wastewater treatment plants
  ASEAN IVO（ASEAN–Japan Innovation Program）2026
  Apr. 2026 - Mar. 2028
  Foo Yee Loo; Tan Wooi Haw; Lo Yew; Chiong; Adeline Chua Seak May; Khor Bee Chin; Alijah Mohd Aris; IDAA Warmadewanthi; I Putu Arta Wibawa; Viet-Anh Nguyen; Nguyen Tra My; Mamoru Oshiki; Chih-Hung Chen
  情報通信研究機構（NICT）, 国際共同研究, Coinvestigator, 2026-04
• 薬剤耐性遺伝子の伝達を可視化する蛍光レポーターアッセイ法の開発
  科学研究費助成事業
  27 Jun. 2025 - 31 Mar. 2027
  押木 守
  日本学術振興会, 挑戦的研究(萌芽), 北海道大学, 25K22850
• 未活用バイオマス資源をバイオ水素へ変換する発酵技術の日印共同開発
  インド若手科学頭脳循環プログラム(LOTUS Programme)
  Apr. 2026 - Mar. 2027
  押木守
  科学技術振興機構(JST), インド若手科学頭脳循環プログラム(LOTUS Programme), 北海道大学, Principal investigator
• 嫌気性アンモニウム酸化細菌の代謝経路の解明と産業利用にむけた基盤創出
  科学研究費助成事業
  Apr. 2023 - Mar. 2027
  押木 守
  日本学術振興会, 基盤研究(B), 北海道大学, Principal investigator, 23K26807
• Creation of novel bioelectrochemical nitrogen conversion technologies to revolutionize the non-sustainable nitrogen cycle
  Grants-in-Aid for Scientific Research
  Apr. 2023 - Mar. 2027
  岡部 聡; 押木 守
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), Hokkaido University, Coinvestigator, 23H00192
• カーボンニュートラル社会を牽引する次世代グリーンテクノロジー人材の育成 ― パーム副産物バイオマスを活用するメタン発酵技術の国際共同開発 ―
  日ASEAN科学技術・イノベーション協働連携事業(NEXUS)
  Mar. 2026 - Dec. 2026
  押木守
  科学技術振興機構(JST), 若手人材交流プログラム（Y-tec）, 北海道大学, Principal investigator
• Innovative nitrogen removal by marine anammox bacteria in recirculating aquaculture systems
  Grants-in-Aid for Scientific Research
  27 Oct. 2020 - 31 Mar. 2024
  金田一 智規; MOJIRI AMIN; 押木 守; 粟田 貴宣
  本研究は、閉鎖循環式陸上養殖の普及・安定化・低コスト化の上で課題であった窒素排水処理を克服するために、塩分環境下で活性のある海洋性アナモックス細菌を用いた排水処理プロセスを導入した次世代型陸上養殖システムをスウェーデン養殖研究センター（ヨーテボリ大学）と共同で開発することである。具体的には、(1)海洋性アナモックス細菌の大量培養方法の確立、(2)前処理として部分硝化を組み込んだアナモックスリアクターの開発、(3)閉鎖循環式陸上養殖の循環海水を対象とした窒素排水処理の最適化と検証を行う。
  2021年度もコロナ禍により海外渡航が難しいため、ヨーテボリ大学とメールによる複数回の実験計画の打ち合わせ、およびWeb会議によるミーティングを行った。さらに日本国内の研究分担者とはメールを中心に一部対面での実験打ち合わせを行った。
  2021年度は、海洋性アナモックス細菌の培地成分として添加している微量金属元素に着目し、それらの種類および濃度を変えて培養を行い、海洋性アナモックス細菌の活性に及ぼす影響を検討した。まず人工海水として用いているシーライフ中に含まれる微量金属元素の濃度をICP-MSによって明らかにした。その後、シーライフ中の微量金属元素のみで海洋性アナモックス細菌の活性が低下するかどうかを検討した。その際、微量金属元素ができるだけ含まれない海水を作製するために高純度試薬を用いて海水を合成した実験系も構築し、シーライフに微量金属元素を添加した対照系と3つの実験系を比較した。その結果、いずれの実験系においても海洋性アナモックス細菌の活性に差は見られなかった。また、16S rRNA遺伝子に基づく細菌叢解析を行った結果、海洋性アナモックス細菌の構成比は変化しないことがわかった。
  Japan Society for the Promotion of Science, Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Hiroshima University, 20KK0244
• 好気性脱窒反応を促進した下水処理場エアレーションタンク単槽での窒素除去技術の開発
  科学研究費助成事業
  01 Apr. 2020 - 31 Mar. 2024
  荒木 信夫; 川上 周司; 青木 仁孝; 押木 守; 渡利 高大
  好気性脱窒能力を有する活性汚泥微生物群の多様性を明らかにするため、実都市下水処理を行っている標準活性汚泥処理装置のエアレーションタンクから活性汚泥を採取し、NO3-N（濃度：約30 mg-N/L）を含む培地にてバッチ式の集積培養を繰り返し行った。最終的に得られた集積培養系による12時間の培養期間における全窒素（TN）除去率は約2割、NO3-N除去率は約5割であった。この結果から、培地に含まれるNO3-N（濃度：約30 mg-N/L）の一部は、好気性脱窒反応によって除去されている可能性が示唆された。また、既知の好気性脱窒能を有すると報告されているAcinetobacter属やComamonas属の細菌が本実験で得られた集積培養系においても好気性脱窒反応を担っている可能性が、16S rRNA遺伝子を標的としたアンプリコンシーケンス解析により示された。
  活性汚泥内での原位置（in-situ）での好気性脱窒反応の速度を評価する手法として、15N標識硝酸（15NO3）を用いて15N標識窒素ガス（15-15N2）の生成速度を評価し、好気性脱窒速度を測定する方法を開発した。本年度は好気性脱窒細菌として知られているParacoccus属細菌を用いて、本手法の検証を行なった。その結果、試験に供したParacoccus属細菌が好気条件下で15N標識硝酸から生成した窒素ガス（30N2ガス）を生成すること、また、好気性脱窒活性がDO濃度に強い影響をうけることを明らかにした。
  日本学術振興会, 基盤研究(B), 20H02290
• 嫌気的硫黄酸化と電子伝達を活性化した生物学的水処理技術の構築
  科学研究費助成事業
  01 Apr. 2020 - 31 Mar. 2023
  山口 隆司; 幡本 将史; 押木 守; 渡利 高大
  開発する水処理システムは、システム前段装置として途上国で普及している上昇流嫌気性汚泥床（UASB）、後段装置として申請者らが開発を進めてきた下降流懸架式スポンジ型好気性反応槽（DHS）を組み合わせた構成である。システムの有機物（BOD）分解者としては、メタン生成古細菌、脱窒素細菌、BOD酸化菌に加えて、硫黄酸化還元サイクルの微生物も積極的に活用することとしている。システム前段UASBは、下水を押出流れで供給する形式とし、内部に直接異種間電子伝達促進ゲル担体を投入する。後段DHSは，スポンジ担体利用散水型装置であり、BOD酸化と硝化を図る。硫黄サイクル微生物によるBOD除去は、特に、低温時に有用となる。本システムを通年連続運転し、黄サイクル微生物によるBOD除去および直接異種間電子伝達促進ゲル担体の制御方法を確立していくものである。特に、保持微生物の評価のための研究として下記を進めた。
  ◎ 嫌気的硫黄酸化について高活性な微生物を培養するため連続式培養装置運転。
  ◎ 嫌気的硫黄酸化の発現汚泥を対象に次世代シーケンサーを用いた網羅的解析。
  ◎ 可視化に成功した嫌気的硫黄酸化微生物の候補は、シングルセルゲノム解析により遺伝子レベルでの機能解明を行ための準備。
  当該年度は、未知な事象である「嫌気的硫黄酸化」に関する電子伝達に関わる反応メカニズムの解明と、低温条件下での嫌気的硫黄酸化還元の制御に関する知見収集が大幅に進む実績を得た。
  日本学術振興会, 基盤研究(A), 長岡技術科学大学, 20H00641
• 新規好気性アンモニア酸化反応の分子メカニズム解明に向けた触媒分子の分離と機能解明
  日本学術振興会科学研究費補助金 基盤研究C
  Apr. 2019 - Mar. 2022
  押木守
  日本学術振興会, Principal investigator, Competitive research funding
• ゲノムマイニングおよび培養試験から存在が見いだされた新規な亜硝酸還元酵素の正体と機能をつきとめる
  Apr. 2018 - Mar. 2020
  押木守
  公益財団法人発酵研究所一般研究助成, Principal investigator, Competitive research funding
• 水処理微生物のバイオインフォマティクス
  Jul. 2016 - Jun. 2019
  福島寿和
  日本製鉄株式会社(旧 新日鐵住金株式会社)・電気通信大学, Competitive research funding
• 分子・物質合成プラットフォーム利用促進事業: 嫌気性アンモニウム酸化(anammox)細菌におけるヒドラジンN2H4分子の細胞イメージング
  Apr. 2018 - Mar. 2019
  押木守
  千歳科学技術大学, Principal investigator, Competitive research funding
• 窒素循環に新たな1ページを加える第三の窒素ガス生成経路を担う微生物群の同定
  日本学術振興会科学研究費補助金 若手(B)
  Apr. 2017 - Mar. 2019
  押木守
  日本学術振興会, Principal investigator, Competitive research funding
• エネルギー資源を生み出す排水処理技術の開発：液体燃料ヒドラジンN2H4の生産
  Sep. 2017 - Mar. 2018
  押木守
  公益財団法人インテリジェント・コスモス学術振興財団, Principal investigator, Competitive research funding
• ヒドラジンをエネルギーとして生産する新規な窒素含有排水処理法の開発
  第一回イムラ・ジャパン賞
  Apr. 2017 - Mar. 2018
  押木守
  イムラ・ジャパン株式会社, Principal investigator, Competitive research funding
• 膜分離技術を利用した脱塩素呼吸Dehalococcoides属細菌の高密度培養による超高速有機塩素嫌気分解リアクターの構築
  育英奨学会研究助成金
  Apr. 2016 - Mar. 2017
  押木守
  公益財団法人ユニオンツール, Principal investigator, Competitive research funding
• ナノ流路集積デバイスを用いた病原性微生物・ウイルスの迅速定量
  Apr. 2015 - Mar. 2016
  押木守
  公益財団法人 内田エネルギー科学振興財団, Principal investigator, Competitive research funding
• 超省エネ都市下水処理の安定性の強化にむけた嫌気性原生動物データベース(PD2)の整備
  Apr. 2015 - Mar. 2016
  押木守
  公益財団法人 前田記念工学振興財団, Principal investigator, Competitive research funding
• ナノ流路デバイスを用いた病原性微生物の迅速検査技術の開発
  研究プロジェクト経費若手研究
  Apr. 2015 - Mar. 2016
  押木守
  独立行政法人国立高等専門学校機構, Principal investigator, Competitive research funding
• 鉄還元型嫌気性アンモニア酸化プロセスによる超省エネルギーかつ省コストな窒素除去技術の創出
  若手研究助成
  Apr. 2014 - Mar. 2016
  押木守
  公益財団法人鉄鋼環境基金, Principal investigator, Competitive research funding
• 都市下水処理UASB内への嫌気性原生動物の高濃度培養技術の開発
  日本学術振興会科学研究費補助金 基盤研究(B)
  Apr. 2013 - Mar. 2016
  荒木信夫
  日本学術振興会, Competitive research funding
• 超省エネルギー型下水処理プロセスにおける病原性微生物の動態
  Oct. 2014 - Mar. 2015
  押木守
  高専−長岡技科大共同研究助成, Principal investigator, Competitive research funding
• 水田土壌由来N2Oガスの生成経路および生成細菌の同定
  Jul. 2014 - Mar. 2015
  押木守
  公益財団法人山口育英奨学会, Principal investigator, Competitive research funding
• アンモニア酸化細菌による亜酸化窒素(N2O)生成機構の解明
  育英奨学会研究助成金
  Apr. 2014 - Mar. 2015
  押木守
  公益財団法人ユニオンツール, Principal investigator, Competitive research funding
• メタボローム解析に基づくANAMMOXプロセスの高効率・安定化技術の創出
  日本学術振興会科学研究費補助金 特別研究員奨励費
  Apr. 2011 - Mar. 2013
  押木守
  日本学術振興会, Principal investigator, Competitive research funding
• 有機物の一時貯蔵によって減少する脱窒量の把握とその改善に向けた検討
  日本学術振興会科学研究費補助金 研究活動スタート支援
  Apr. 2009 - Mar. 2011
  押木守
  日本学術振興会, Principal investigator, Competitive research funding
• 活性汚泥におけるPHA蓄積細菌群集の分子生物学的手法による解析
  日本学術振興会科学研究費補助金 特別研究員奨励費
  Apr. 2007 - Mar. 2008
  押木守
  日本学術振興会, Principal investigator, Competitive research funding

• 微生物群の特定方法
  Patent right, 福島寿和; 中川淳一; 加藤敏朗; 川野秀一; 押木守, 日本製鉄株式会社
  特願特願2019-105590, 05 Jun. 2019
  特開2020-036579, 12 Mar. 2020
  特許7299485
• 遺伝子又は該遺伝子産物の定量方法及びモニタリング方法
  Patent right, 福島寿和; 中川淳一; 押木守; 川野秀一, 日本製鉄株式会社
  特願特願2020-100233, 09 Jun. 2020
  特許特開2021-193883

• 日本酒醸造法に関する新規な醸造技術の提案
  17 Nov. 2018 - 17 Nov. 2018
  Advisor, Organizing member
  発酵を科学するアイディアコンテスト
  第二回 発酵を科学するアイディアコンテスト

• 廃棄物処理に関する啓蒙活動
  01 Oct. 2018
  ケーブル4K
  テレビ番組 テクノ探検隊「都市金山を掘り起こせ ～不要になった基盤から金を回収～」
  [Media report]