博士 (農学), 北海道大学

魚肉タンパク質

慢性炎症

非感染疾患

紅藻

未利用水産資源

Life Science, Food sciences

Life Science, Aquatic bioproduction science, 水産食品科学分野

Bachelor's degree program, School of Fisheries Sciences

Master's degree program, Graduate School of Fisheries Sciences

Doctoral (PhD) degree program, Graduate School of Fisheries Sciences

Nov. 2021, 8th International symposium East Asia Fisheries Technologists Association 2021, Best Oral Award
Enhanced anti-inflammatory activity of glycated salmon myofibrillar protein with reducing sugars containing carboxyl group,
Wenzhao Li*, Ga-Hyun Joe, Hiroki Saeki

Nov. 2021, 8th International symposium East Asia Fisheries Technologists Association 2021, Best Oral Award
Development of bio-functionally enhanced collagen by alginate oligosaccharide glycation
Boxue Yang;Ga-Hyun Joe;Hiroki Saeki

Dec. 2020, 2020年度日本農芸化学会北海道支部／第50回 日本栄養・食糧学 会北海道支部合同学術講演会, 若手奨励賞
スケトウダラ筋肉水溶性タンパク質はマクロファージの受容体発現の変動を介してLPS誘導性炎症を抑制する
大野 雅貴1,東(西澤) 瑞穂2,高橋 義宣2,小西 達也2,清水 裕1,趙 佳賢1,佐伯 宏樹1 (1北大院水,2マルハニチロ 中央研究所)

May 2017, The International Conference of Korean Society of Food Science of Animal Resources and 49th Annual Meeting, The Excellence Poster Presentation Award

Nov. 2016, 日本農芸化学会･北海道支部会, 学生会員奨励賞受賞講演
Modulation of inflammatory responses by megalo-type isomaltoglucosaccharides
趙 佳賢

Aug. 2016, 日本農芸化学会北海道支部平成28年度第1回講演会, 優秀発表賞
趙 佳賢

Nov. 2015, The 6th International Conference on Food Factors, Poster Award受賞
趙 佳賢

Reduction of salmon roe allergy risk through fish meat consumption using triploidization technology
Saki Watanabe; Yukiho Sasaoka; Gahyun Joe; Hiroki Saeki; Yutaka Shimizu
Fisheries Science, Springer Science and Business Media LLC, 18 Jul. 2025, [Peer-reviewed]
Scientific journal

Dietary isomaltomegalosaccharides ameliorate lipopolysaccharide-induced endotoxemia in rats
Ga-Hyun Joe; Shota Hori; Akari Takeuchi; Sena Ishikawa; Kanta Pora; Fumika Yokoyama; Takahisa Iizuka; Yoshinori Fujimoto; Weeranuch Lang; Atsuo Kimura; Hiroshi Hara; Satoshi Ishizuka
Food Science and Technology Research, 31, 3, 279, 285, Japanese Society for Food Science and Technology, 2025, [Peer-reviewed], [Lead author]
Scientific journal

Enhanced anti-inflammatory effect of fish myofibrillar protein by introducing pectin oligosaccharide and its molecular mechanisms
Wenzhao Li; Hiroki Saeki; Boxue Yang; Yutaka Shimizu; Ga-Hyun Joe
Food Chemistry, 141082, 141082, Elsevier BV, Sep. 2024, [Peer-reviewed], [Last author, Corresponding author]
Scientific journal

Fish myofibrillar protein glycated with uronic acid‐type reducing sugar via Maillard reaction attenuates toll‐like receptor 4 signalling pathway activation and suppresses lipopolysaccharide‐stimulated inflammation in RAW 264.7 macrophages
Wenzhao Li; Hiroki Saeki; Boxue Yang; Yutaka Shimizu; Ga‐Hyun Joe
International Journal of Food Science and Technology, Wiley, 07 Apr. 2024, [Peer-reviewed], [Last author, Corresponding author]
Scientific journal, Summary

Our previous study found that glycation with uronic acid‐type reducing sugar enhanced the anti‐inflammatory effect of fish myofibrillar protein (Mf) via the Maillard reaction, and the molecular mechanism of enhanced anti‐inflammatory effect was investigated in this study. Mf was glycated with various reducing sugars at 60 °C and 35% relative humidity. After pepsin‐trypsin digestion, the anti‐inflammatory effects were evaluated by assessing secretions of pro‐inflammatory cytokines and expressions of LPS‐stimulated signalling pathway‐related genes in RAW 264.7 macrophages. Mf glycated with uronic acid‐type reducing sugars: glucuronic acid, galacturonic acid and alginate oligosaccharide, suppressed expression of cd14 and further suppressed expressions of tlr4 and myd88 comparing with intact and glycated Mf with glucose and galactose. Furthermore, uronic acid‐glycated Mf significantly suppressed expressions and secretions of pro‐inflammatory cytokines. The results indicate that uronic acid‐type glycation enhances anti‐inflammatory effect of Mf by attenuating expression of cd14 and enhancing the Mf‐induced suppression in TLR4‐MyD88‐dependent inflammatory signalling pathway.

Safety evaluation of salmon muscle consumption in patients with salmon roe allergy
Yutaka Shimizu; Ga-hyun Joe; Hiroki Saeki
Fisheries Science, Springer Science and Business Media LLC, 08 Mar. 2024, [Peer-reviewed]
Scientific journal

Maillard-type glycated collagen with alginate oligosaccharide suppresses inflammation and oxidative stress by attenuating the expression of LPS receptors Tlr4 and Cd14 in macrophages
Boxue Yang; Wenzhao Li; Hiroki Saeki; Yutaka Shimizu; Ga-Hyun Joe
Food & Function, Royal Society of Chemistry (RSC), Feb. 2024, [Peer-reviewed], [Last author, Corresponding author]
Scientific journal, 18h AO-glycated collagen (C-AO) reduced LPS receptor expression, enhancing anti-inflammatory and anti-oxidant effects in macrophages. C-AO shows potential in disease prevention and inflammation management.

Impact of backslopping in TERASI manufacture, as an improving method to reduce shrimp allergenicity
Ulfah Amalia; Yutaka Shimizu; Ga-Hyun Joe; Hiroki Saeki
Food Chemistry, 434, 137491, 137491, Elsevier BV, Feb. 2024, [Peer-reviewed]
Scientific journal

Water-soluble protein from walleye pollock (Gadus chalcogrammus) suppresses lipopolysaccharide-induced inflammation by attenuating TLR4–MyD88 expression in macrophages
Masataka Ono; Satomi Watari; Mizuho Nishizawa-Higashi; Tatsuya Konishi; Yoshinori Takahashi; Hiroki Saeki; Ga-Hyun Joe
Food Chemistry: Molecular Sciences, 6, 100165, 100165, Elsevier BV, Jul. 2023, [Peer-reviewed], [Last author, Corresponding author]
Scientific journal

Glycation with uronic acid-type reducing sugar enhances the anti-inflammatory activity of fish myofibrillar protein via the Maillard reaction
Wenzhao Li; Boxue Yang; Ga-Hyun Joe; Yutaka Shimizu; Hiroki Saeki
Food Chemistry, 407, 135162, 135162, Elsevier BV, May 2023, [Peer-reviewed]
Scientific journal

Development of a Sequential Fractionation-and-Recovery Method for Multiple Anti-Inflammatory Components Contained in the Dried Red Alga Dulse (Palmaria palmata)
Ga-Hyun Joe; Masafumi Masuoka; Ryosuke Reisen; Seiya Tanaka; Hiroki Saeki
Marine Drugs, 21, 5, 276, 276, MDPI AG, 28 Apr. 2023, [Peer-reviewed], [Lead author]
Scientific journal, A separation process was established to sequentially fractionate and recover three anti-inflammatory components derived from sugars, phycobiliprotein, and chlorophyll from the hot-air-dried thalli of the red alga dulse (Palmaria palmata). The developed process consisted of three steps, without the use of organic solvents. In Step I, the sugars were separated by disrupting the cell wall of the dried thalli with a polysaccharide-degrading enzyme, and a sugar-rich extract (E1) was obtained by precipitating the other components, which were simultaneously eluted by acid precipitation. In Step II, the residue suspension from Step I was digested with thermolysin to obtain phycobiliprotein-derived peptides (PPs), and a PP-rich extract (E2) was obtained by separating the other extracts using acid precipitation. In Step III, solubilized chlorophyll was obtained by heating the residue, which was acid-precipitated, neutralized, and re-dissolved to concentrate the chlorophyll-related components (Chls)-rich extract (E3). These three extracts suppressed inflammatory-cytokine secretion by lipopolysaccharide (LPS)-stimulated macrophages, confirming that the sequential procedure had no negative effects on the activities of any of the extracts. The E1, E2, and E3 were rich in sugars, PPs, and Chls, respectively, indicating that the anti-inflammatory components were effectively fractionated and recovered through the separation protocol.

Advanced Utilization of By-Product from the Sturgeon Aquaculture Industry: Optimum Preparation of Notochord-Derived Multifunctional Peptides by Glycation using the Maillard Reaction
Boxue Yang; Wenzhao Li; Asuka Ishii; Ga-hyun Joe; Yutaka Shimizu; Yasuaki Takagi; Hiroki Saeki
Waste and Biomass Valorization, Springer Science and Business Media LLC, 24 Mar. 2023, [Peer-reviewed]
Scientific journal

Food safety evaluation of commercial Terasi, Indonesian fermented shrimp paste, from the viewpoint of food allergy
Ulfah Amalia; Yutaka Shimizu; Ga-Hyun Joe; Hiroki Saeki
Fisheries Science, Springer Science and Business Media LLC, 18 Jan. 2023, [Peer-reviewed]
Scientific journal

A diet supplemented with cholic acid elevates blood pressure accompanied by albuminuria in rats
Tomoko Shimoda; Hidehisa Shimizu; Wakana Iwasaki; Hongxia Liu; Yoshie Kamo; Koji Tada; Taketo Hanai; Shota Hori; Ga-Hyun Joe; Yasutake Tanaka; Masao Sato; Hitoshi Miyazaki; Satoshi Ishizuka
Bioscience, Biotechnology, and Biochemistry, 87, 4, 434, 441, Oxford University Press (OUP), 09 Jan. 2023, [Peer-reviewed]
Scientific journal

Comparison of Maillard-Type Glycated Collagen with Alginate Oligosaccharide and Glucose: Its Characterization, Antioxidant Activity, and Cytoprotective Activity on H2O2-Induced Cell Oxidative Damage
Boxue Yang; Ga-Hyun Joe; Wenzhao Li; Yutaka Shimizu; Hiroki Saeki
Foods, 11, 15, 2374, 2374, MDPI AG, 08 Aug. 2022, [Peer-reviewed]
Scientific journal, To improve the antioxidant activity of collagen molecules using Maillard-type glycation, the relation between antioxidant activity and progress indexes for the Maillard reaction must be understood. In this study, lyophilized tilapia scale collagen was mixed with a half weight of alginate oligosaccharide (AO) or glucose and incubated at 60 °C and 35% relative humidity for up to 18 h to produce the Maillard-type glycated collagen (C-AO and C-Glu, respectively). As glycation progressed, the amount of conjugated sugar coupled with UV-vis absorbance at 294 nm and 420 nm increased more rapidly in C-Glu than in C-AO, and the available lysine decreased rapidly in C-Glu compared with C-AO. The early-to-middle- and late-stage products of the Maillard reaction were involved in enhanced antioxidant activity of digested C-AO and digested C-Glu, respectively. Additionally, C-AO acquired the antioxidant activity without marked available lysine loss. The cytoprotective effect of collagen in H2O2-induced damage was enhanced by glycation, achieved by reducing malondialdehyde content and increasing superoxide dismutase and catalase activities. These results indicate that AO is an excellent reducing sugar that enhances the health benefits of collagen without excessive loss of lysine, which is a nutritional problem of the Maillard-type glycation.

Effect of drying treatment on the extractability and anti-inflammatory function of photosynthesis-related components in dulse Palmaria palmata and their efficient recovery from dried thallus
Daichi Sugita; Ga-Hyun Joe; Masafumi Masuoka; Yasuyuki Konishi; Hiroki Saeki
Fisheries Science, Springer Science and Business Media LLC, 20 Jul. 2022, [Peer-reviewed]
Scientific journal

Dietary supplementation with okara and Bacillus coagulans lilac-01 improves hepatic lipid accumulation induced by cholic acids in rats
Yeonmi Lee; Yasutake Tanaka; Wakana Iwasaki; Fumika Yokoyama; Ga-Hyun Joe; Misaki Tsuji; Takuma Nose; Koji Tada; Taketo Hanai; Shota Hori; Hidehisa Shimizu; Kimiko Minamida; Kazunori Miwa; Satoshi Ishizuka
Journal of Functional Foods, 90, 104991, 104991, Elsevier BV, Mar. 2022, [Peer-reviewed]
Scientific journal

Nutritional value of Pacific herring bone co-ingested with fish meat as a calcium source in retort seafood
Ga-Hyun Joe; Masataka Ono; Koji Tsuji; Tadaaki Takeda; Hiroki Saeki
Fisheries Science, Springer Science and Business Media LLC, 12 Jul. 2021, [Peer-reviewed], [Lead author]
Scientific journal

12α-Hydroxylated bile acid enhances accumulation of adiponectin and immunoglobulin A in the rat ileum
Reika Yoshitsugu; Hongxia Liu; Yoshie Kamo; Akari Takeuchi; Ga-Hyun Joe; Koji Tada; Keidai Kikuchi; Nobuyuki Fujii; Shinri Kitta; Shota Hori; Manami Takatsuki; Hitoshi Iwaya; Yasutake Tanaka; Hidehisa Shimizu; Satoshi Ishizuka
Scientific Reports, 11, 1, 12939, 12939, Springer Science and Business Media LLC, 21 Jun. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, We previously reported that dietary supplementation with cholic acid (CA), the primary 12α-hydroxylated (12αOH) bile acid (BA), reduces plasma adiponectin concentration in rats. The aim of this study was to examine the distribution of adiponectin in the body of CA-fed rats and its influence on mucosal immunoglobulin A concentration in the intestine. Rats were fed a diet supplemented with or without CA (0.5 g CA/kg diet) for 13 weeks. A reduction in plasma adiponectin level was observed from week 3. At the end of the experiment, the CA diet reduced plasma adiponectin concentration both in the portal and aortic plasma. Accumulation of adiponectin was accompanied by an increase in cadherin-13 mRNA expression in the ileal mucosa of CA-fed rats. No increase was observed in adiponectin mRNA expression in the ileal and adipose tissues of the CA-fed rats. Immunoglobulin A concentration in the ileal mucosa was elevated in the CA-fed rats and was correlated with the ileal adiponectin concentration. 12αOH BAs may modulate mucosal immune response that are involved in the accumulation of adiponectin in the ileum.

Improvement of anti-inflammatory activity of salmon muscle peptides by conjugation with alginate oligosaccharide and recovery of the active fraction using ampholyte-free isoelectric focusing
Kunichika Sugihara; Mizuho Nishizawa-Higashi; Ga-Hyun Joe; Yutaka Onishi; Yutaka Shimizu; Hiroki Saeki
Fisheries Science, 87, 4, 569, 577, Springer Science and Business Media LLC, 26 Apr. 2021, [Peer-reviewed]
Scientific journal

12α-Hydroxylated bile acid induces hepatic steatosis with dysbiosis in rats
Ja-Young Lee; Hidehisa Shimizu; Masahito Hagio; Satoru Fukiya; Masamichi Watanabe; Yasutake Tanaka; Ga-Hyun Joe; Hitoshi Iwaya; Reika Yoshitsugu; Keidai Kikuchi; Misaki Tsuji; Nanako Baba; Takuma Nose; Koji Tada; Taketo Hanai; Shota Hori; Akari Takeuchi; Yumiko Furukawa; Bungo Shirouchi; Masao Sato; Tadasuke Ooka; Yoshitoshi Ogura; Tetsuya Hayashi; Atsushi Yokota; Satoshi Ishizuka
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1865, 12, 158811, 158811, Elsevier BV, Dec. 2020, [Peer-reviewed]
Scientific journal

A low coefficient of variation in hepatic triglyceride concentration in an inbred rat strain.
Tomoko Shimoda; Shota Hori; Kenta Maegawa; Akari Takeuchi; Yeonmi Lee; Ga-Hyun Joe; Yasutake Tanaka; Hidehisa Shimizu; Satoshi Ishizuka
Lipids in health and disease, 19, 1, 137, 137, 16 Jun. 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, BACKGROUND: Inbred strains are characterized by less genetic variation, which suggests usefulness of inbred strains for evaluations of various parameters. In this study, experimental reproducibility in several parameters was compared between an outbred Wistar rat and Wistar King A Hokkaido (WKAH/HkmSlc) rat, the inbred strain that is originated from Wistar rats. METHODS: Difference of variations was investigated in parameters of body compositions and liver functions such as body weight, liver weight, liver triglycerides (TG), liver cholesterol and plasma alanine aminotransferase activity (ALT) between WKAH rats and outbred Wistar rats by using the coefficient of variation (CV). RESULTS: There was no difference in the CVs of body weight and relative liver weight between WKAH and Wistar rats. The CVs of body weight and relative liver weight were below 10% in both WKAH and Wistar rats. The CVs of TG, cholesterol, and ALT in Wistar rats were between 30 and 40%, whereas those in WKAH rats were between 10 and 25%. A low CV level of TG was observed in WKAH rats compared to that in Wistar rats regardless of the duration of the experimental period in those rat strains. CONCLUSION: The low CV values in metabolic parameters involved in liver functions in the inbred rats suggested an advantage of using inbred rather than outbred rats for the evaluation of liver lipid metabolism.

Combination of soya pulp and Bacillus coagulans lilac-01 improves intestinal bile acid metabolism without impairing the effects of prebiotics in rats fed a cholic acid-supplemented diet
Yeonmi Lee; Reika Yoshitsugu; Keidai Kikuchi; Ga-Hyun Joe; Misaki Tsuji; Takuma Nose; Hidehisa Shimizu; Hiroshi Hara; Kimiko Minamida; Kazunori Miwa; Satoshi Ishizuka
BRITISH JOURNAL OF NUTRITION, 116, 4, 603, 610, Aug. 2016, [Peer-reviewed]
English, Scientific journal

Megalo-type alpha-1,6-glucosaccharides induce production of tumor necrosis factor alpha in primary macrophages via toll-like receptor 4 signaling
Ga-Hyun Joe; Midori Andoh; Aki Shinoki; Weeranuch Lang; Yuya Kumagai; Juri Sadahiro; Masayuki Okuyama; Atsuo Kimura; Hidehisa Shimizu; Hiroshi Hara; Satoshi Ishizuka
BIOMEDICAL RESEARCH-TOKYO, 37, 3, 179, 186, 2016, [Peer-reviewed], [Lead author]
English, Scientific journal

Activity of ERK regulates mucin 3 expression and is involved in undifferentiated Caco-2 cell death induced by 3-oxo-C-12-homoserine lactone
Hidehisa Shimizu; Nanako Baba; Takuma Nose; Ryoko Taguchi; Shinya Tanaka; Ga-Hyun Joe; Hideaki Maseda; Nobuhiko Nomura; Masahito Hagio; Ja-Young Lee; Satoru Fukiya; Atsushi Yokota; Satoshi Ishizuka; Hitoshi Miyazaki
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 79, 6, 937, 942, Jun. 2015, [Peer-reviewed]
English, Scientific journal

Diet supplementation with cholic acid promotes intestinal epithelial proliferation in rats exposed to gamma-radiation
Masahito Hagio; Hidehisa Shimizu; Ga-Hyun Joe; Manami Takatsuki; Maiko Shiwaku; Hong Xu; Ja-Young Lee; Nobuyuki Fujii; Satoru Fukiya; Hiroshi Hara; Atsushi Yokota; Satoshi Ishizuka
TOXICOLOGY LETTERS, 232, 1, 246, 252, Jan. 2015, [Peer-reviewed]
English, Scientific journal

Mucin 3 is involved in intestinal epithelial cell apoptosis via N-(3-oxododecanoyl)-L-homoserine lactone-induced suppression of Akt phosphorylation
Ryoko Taguchi; Shinya Tanaka; Ga-Hyun Joe; Hideaki Maseda; Nobuhiko Nomura; Junji Ohnishi; Satoshi Ishizuka; Hidehisa Shimizu; Hitoshi Miyazaki
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 307, 2, C162, C168, Jul. 2014, [Peer-reviewed]
English, Scientific journal

Acyl-homoserine lactones suppresses IEC-6 cell proliferation and increase permeability of isolated rat colon
Ga-Hyun Joe; Midori Andoh; Mikako Nomura; Hitoshi Iwaya; Jae-Sung Lee; Hidehisa Shimizu; Youhei Tsuji; Hideaki Maseda; Hitoshi Miyazaki; Hiroshi Hara; Satoshi Ishizuka
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 78, 3, 462, 465, 2014, [Peer-reviewed], [Lead author]
English, Scientific journal

Ingestion of Megalo-type Isomaltoglucosaccharides Ameliorates Lipopolysaccharide-induced Acute Liver Injury in Rats
Ga-Hyun Joe; Takahisa Iizuka; Yoshinori Fujimoto; Atsuo Kimura; Hiroshi Hara; Satoshi Ishizuka, FASEB JOURNAL, 31, Apr. 2017
English, Summary international conference

胆汁酸誘導性未病状態の脱脂乳チーズによる改善作用
高橋勇太郎; LEE Dongguen; LEE Yeonmi; JOE Gahyun; 加茂佳恵; 多田幸司; 堀将太; 花井健人; 竹内あかり; 田中愛健; 清水英寿; 若松純一; 原博; 石塚敏; 玖村朗人, 日本農芸化学会大会講演要旨集(Web), 2017, ROMBUNNO.2A08a08 (WEB ONLY), 05 Mar. 2017
Japanese

A reduction of myeloperoxidase and interleukin-10 expressions in mesenteric lymph nodes of the rats fed a cholic acid-supplemented diet
Ga-Hyun Joe; Shota Hori; Hidehisa Shimizu; Hiroshi Hara; Satoshi Ishizuka, FASEB JOURNAL, 30, Apr. 2016
English, Summary international conference

Deoxycholic acid is involved in the initiation and progression of atherosclerosis.
Hidehisa Shimizu; Masahito Hagio; Reika Yoshitsugu; Keidai Kikuchi; Ga Hyun Joe; Hiroshi Hara; Satoshi Ishizuka, FASEB JOURNAL, 27, Apr. 2013
English, Summary international conference

Oral administration of cholic acid modulates the gut microbiota and epithelial proliferation
Satoshi Ishizuka; Manami Takatsuki; Maiko Shiwaku; Masahito Hagio; Reika Yoshitsugu; Keidai Kikuchi; Nobuyuki Fujii; Ga‐Hyun Joe; Hidehisa Shimizu; Hiroshi Hara; Satoru Fukiya; Atsushi Yokota, The FASEB Journal, 27, S1, Apr. 2013
English

未利用・廃棄水産資源の活用と SDGs への 貢献
Ga-Hyun Joe
未利用資源活用セミナー 畜産・水産物における未利用資源活用の取り組み, 14 Mar. 2023

Increased PTH secretion and Ca absorption rate due to fish bone intake in rats
Ga-Hyun JOE; Kazuki FUJIMOTO; Hirofumi TAKEDA; Hiroki SAEKI
22nd IUNS-ICN International Congress of Nutrition in Tokyo, Japan, 06 Dec. 2022
06 Dec. 2022 - 11 Dec. 2022

健康機能から見た未利用・廃棄水産資源の高度利用
Ga-Hyun Joe
日本栄養·食糧学会 北海道支部 シンポジウム「北海道におけるこれからの栄養・食糧学」, 05 Mar. 2022

熱風乾燥した紅藻ダルスに含まれる各種抗炎症成分の個別抽出法の開発
増岡 雅文,岸 夏海,趙 佳賢,清水 裕,佐伯 宏樹,
第73回日本栄養・食糧学会大会, 19 May 2019, Japanese, Oral presentation
[Domestic Conference]

アルギン酸オリゴ糖修飾したシロザケ筋肉ペプチド間における抗炎症作用の比較
大西 豊; 李; 趙 佳賢; 佐伯 宏樹
日本栄養・食糧学会北海道・東北合同支部大会および公開シンポジウム, 18 Nov. 2018

熱風乾燥ダルスのプロテアーゼ処理条件が各種抗炎症成分の抽出挙動に及ぼす影響
岸 夏海,増岡 雅文,趙 佳賢,清水 裕,佐伯 宏樹
日本栄養・食糧学会北海道・東北合同支部大会および公開シンポジウム, 18 Nov. 2018, Japanese, Oral presentation
[Domestic Conference]

熱風乾燥した紅藻ダルスからの抗炎症成分の効率的な抽出法の開発
増岡 雅文,岸 夏海,趙 佳賢,清水 裕,佐伯 宏樹
日本栄養・食糧学会北海道・東北合同支部大会および公開シンポジウム, 18 Nov. 2018, Japanese, Oral presentation
[Domestic Conference]

Ingestion of skim milk cheese modulates bile acid metabolism and cholic acid-induced disorders in rats.
GA-HYUN JOE
The International Conference of Korean Society of Food Science of Animal Resources and 49th Annual Meeting, 18 May 2017, English, Poster presentation
[International presentation]

Ingestion of megalo-type isomaltoglucosaccharides ameliorates lipopolysaccharide-induced acute liver injury in rats.
GA-HYUN JOE
Experimental Biology, 14 Apr. 2017, English, Poster presentation
[International presentation]

胆汁酸摂取ラット回腸粘膜におけるIgA産生の増加
趙 佳賢
日本農芸化学会2017年度大会（京都）, 17 Mar. 2017, Japanese, Oral presentation
[Domestic Conference]

胆汁酸誘導性未病状態の脱脂乳チーズによる改善作用
趙 佳賢
日本農芸化学会2017年度大会（京都）, 17 Mar. 2017, Japanese, Oral presentation
[Domestic Conference]

胆汁酸誘導性病態の発症に及ぼす脱脂乳チーズ摂取の作用
趙 佳賢
日本農芸化学会北海道支部平成28年度第1回講演会（函館）, 06 Aug. 2016, Japanese, Oral presentation
[Domestic Conference]

Cholic acid promotes gut epithelial proliferation in rats exposed to gamma-radiation.
GA-HYUN JOE
XXIV International Bile Acid Meeting: Bile Acids in Health and Disease, Düsseldorf, Germany, 17 Jun. 2016, English, Poster presentation
[International presentation]

A reduction of myeloperoxidase and interleukin-10 expressions in mesenteric lymphnodes of the rats fed a cholic acid-supplemented diet.
GA-HYUN JOE
Experimental Biology, 02 Apr. 2016, English, Poster presentation
[International presentation]

A diet supplemented with cholic acid induced hepatic steatosis without apparent inflammation and reduced myeloperoxidase and interleukin-10 expressions in the mesenteric lymph nodes in rats.
GA-HYUN JOE
第21回Hindgut Club Japanミーティング（東京）, 05 Dec. 2015, English, Oral presentation
[Domestic Conference]

Isomaltomegalosaccharides modulation rat macrophage functions in primary culture and cytokine expression in rat intestinal mucosa.
GA-HYUN JOE
The 6th International Conference on Food Factors, 22 Nov. 2015, English, Poster presentation
[International presentation]

Modulation of rat macrophage functions by isomaltomegalosaccharides.
GA-HYUN JOE
12th Asian Congress of Nutrition, 14 May 2015, English, Poster presentation
[International presentation]

Modulation of immune-related gene expressions in mesenteric lymph nodes of the rats fed cholic acid-supplemented diet.
GA-HYUN JOE
日本農芸化学会大会（岡山）, 26 Mar. 2015, English, Oral presentation
[Domestic Conference]

Deoxycholic acid is involved in the initiation and progression of atherosclerosis
GA-HYUN JOE
Experimental Biology 2013, 21 Apr. 2014, English, Poster presentation
[International presentation]

Ingestion of cholic acid modulates the gut microbiota and intestinal epithelial proliferation.
GA-HYUN JOE
Obesity Week 2013, 11 Nov. 2013, English, Poster presentation
[International presentation]

Evaluation of isolation and culture procedure for sodium periodate-induced peritoneal elicited cells as a model system of functional analysis in primary macrophages.
GA-HYUN JOE
日本農芸化学会北海道支部夏期シンポジウム（旭川）, 10 Aug. 2013, English, Poster presentation
[Domestic Conference]

Cytokine production in immune cells in response to megalo type isomaltosylglucosaccharides
GA-HYUN JOE
日本栄養・食糧学会第67回大会（名古屋）, 24 May 2013, English, Oral presentation
[Domestic Conference]

Supplementation of cholic acid in enterohepatic circulation modulates the gut microbiota and epithelial proliferation.
GA-HYUN JOE
Experimental Biology 2013, 21 Apr. 2013, English, Poster presentation
[International presentation]

TNFα production in mesenteric leukocytes and Raw 264.7 cells in response to megalo type isomaltosylglucosaccharides.
GA-HYUN JOE
第18回Hindgut Club Japanミーティング（東京）, 08 Dec. 2012, English, Oral presentation
[Domestic Conference]

10. Carboxyl group in reducing sugar is an important factor for enhancing anti-inflammatory activity of glycated fish myofibrillar protein
令和3年度公益社団法人日本水産学会春季大会

Carboxyl group in reducing sugar is an important factor for enhancing anti-inflammatory activity of glycated fish myofibrillar protein
令和3年度公益社団法人日本水産学会春季大会

水産食品科学特論Ⅰ, 2024年, 修士課程, 水産科学院

水産食品科学特論Ⅱ, 2024年, 修士課程, 水産科学院

健康と社会, 2024年, 学士課程, 全学教育

食品衛生学実験, 2024年, 学士課程, 水産学部

生産プロセス工学実習, 2024年, 学士課程, 水産学部

Functional analysis of anti-inflammatory protein contained in fish meat water-soluble fraction and its utilization for prevention of non-communicable diseases
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
01 Apr. 2021 - 31 Mar. 2024
佐伯 宏樹; 趙 佳賢
2021年度は，抗炎症機能の詳細な検証・メカニズム解析と，機能タンパク質の特定を計画していたが，利用予定していた分析装置の入手の遅れから，メカニズム解析は追試験のレベルに留まった。そのため，2021ー22年に予定していた水溶性タンパク質画分（WSP）の組成分析を先行実施するとともに，凍結筋肉や工場廃水からWSPを抽出し，抗炎症機能の差異を検討し,WSPのもつ抗炎症機能の安定性を検証した。さらに2022年度の動物実験を踏まえて，メイラード反応を用いた糖鎖導入が，その抗炎症作用におよぼす影響を調査した。
まず，スケトウダラ筋肉の水抽出画分から，等電点沈殿を主体とした生化学的手法によってWSPを調製し，これを電気泳動分画，免疫染色，TOF-MS分析に供して，含有成分の特定をおこなったところ，（1）WSPは解糖系酵素群，ミオシン軽鎖，およびパルブアルブミンから成ることを確認した。続いて（2）スケトウダラの生鮮筋肉，凍結筋肉，さらに冷凍すりみ工場の工場廃液のいずれを原料としても，各WSPはLPS刺激したマウスマクロファージの炎症性サイトカイン産生を抑制した。さらに(3)糖鎖導入はWSPの抗炎症機能を増強することを明らかにできた。
(1)と(2)の結果は，WSPの構成タンパク質との抗炎症機能が原材料の相違によらず維持されていることを示す結果であり，今後，本研究の知見を産業的に展開する上で重要な知見である。また（3）はメイラード反応による糖鎖導入が抗炎症機能を強く増強させる可能性を示した結果であり，2022年度の動物実験計画に反映させることで，本研究の意義を大いに高められる可能性がある。
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 21K05765

Regulation of immune function by dulse (Palmaria palmata) and prevention of non-alcoholic fatty liver disease
Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists
01 Apr. 2021 - 31 Mar. 2024
趙 佳賢
周りを海に囲まれた日本では, 多様な水産資源を食品や加工品として利用しているが, それでも利用されていない水産資源もまだ多く存在する。暖流と寒流の双方の影響を受ける日本沿岸の岩礁域には多様な海藻が生息しているため, その資源量は豊富であるが, 食用種はわずかであり, 代表的な未利用水産資源である。本研究で対象とした未利用紅藻，ダルス (Palmaria palmata)の資源量は，函館市臼尻近郊だけでも，年間数千トンに到達すると推定されている。しかし，コンブ養殖の種苗ロープに繁茂して日光を遮断するため，そのほとんどが除去・廃棄されている。先行研究では，凍結乾燥ダルスに含まれる複数種の抗炎症成分であるフィコビリタンパク質由来ペプチド (PP) とクロロフィルa関連化合物 (Chls)の有効性の可能性が示唆された。
2021年度には，海藻の代表的な加工処理法である熱風乾燥を用いて，ダルスを熱風乾燥により減容化した藻体から，三つの有効成分(糖画分とPP画分，Chls画分)の分画抽出する方法を検討した。さらに，ダルス由来の有効成分が生活習慣病の発症・増悪に深く関与しているマクロファージの免疫応答におよぼす影響を調べた。その結果，未利用水産資源である紅藻ダルスから抽出したPP画分とChls画分が抗炎症効果を持ち，また両画分の作用メカニズムが異なることを明らかにした。まず(1)PP画分は免疫細胞を活性化させ，炎症の早期収束に寄与することで炎症を抑制する。次に(2)Chls画分はLPS受容体をはじめとする異物認識機能を調節することで，炎症を抑制する。このような研究成果はダルス由来のPP画分とChls画分が炎症抑制の機能性食品素材としての利用可能性が高いことを示唆する。
Japan Society for the Promotion of Science, Grant-in-Aid for Early-Career Scientists, Hokkaido University, 21K14912

未利用紅藻・ダルスからの抗炎症糖質の効果的抽出と機能解析
令和３年度研究助成金
Apr. 2021 - Mar. 2022
公益財団法人飯島藤十郎記念食品科学振興財団, 北海道大学, Principal investigator

紅藻ダルスに含まれる免疫機能増強成分の活用
研究助成金
Apr. 2020 - Mar. 2021
公益財団法人北水協会, 北海道大学, Principal investigator

Involvement of immune system in non-obesity fatty liver and disorders
Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows
22 Apr. 2016 - 31 Mar. 2018
趙 佳賢
先行の研究で一次胆汁酸であるコール酸の摂取が脂肪肝を誘導し、未病モデルであることを見出した。この病態モデルでの脂肪肝は1st hit直後の状態で、2nd hit前の単純性脂肪肝であると考えられる。しかしながら、肝臓や血中での炎症反応は見られず、消化管透過性の亢進が確認された。そこで、コール酸負荷による脂肪肝発症メカニズムを調べるため、LPSを2nd hitしてコール酸摂取ラットに投与し、肝炎での悪化を調べた。コール酸を10週間摂取した Fischer344ラットにLPSを皮下に投与、投与6時間後に解剖を行った。その結果、先行研究においてWKAH系統のラットで見られた透過性の亢進は見られなかった。肝臓及び消化管内容物における胆汁酸組成が系統間で異なることより、消化管透過性に影響を及ぼすことが考えられる。一方で肝障害マーカーがコール酸群でさらに増加したことから、コール酸負荷が肝障害の悪化させることが明らかになった。また、そのメカニズムを調べるため、肝臓での炎症関連遺伝子や繊維化マーカの発現を分析した。しかし、LPS刺激でのコール酸摂取による炎症や繊維化関連遺伝子発現には有意差が見られなかった。LPSによる炎症反応に自然免疫細胞であるマクロファージが関与していることから、マクロファージの機能に関与する遺伝子を分析した結果、群間差は見られなかった。本研究では0.05％コール酸摂取による単純性脂肪肝の病態モデルで、LPS刺激による肝障害の悪化が示唆された。高脂肪食の摂取は胆汁酸の分泌を促進させ、加齢により一次胆汁酸であるコール酸の割合が増加する。また、高脂肪食による体内でのLPS濃度の増加や加齢による免疫システムの変化が知られている。したがって、胆汁酸摂取による単純性脂肪肝の発症が「未病」状態であることを強く示唆するだけでなく、炎症や代謝疾患の発症や悪化に繋がる可能性も示している。
Japan Society for the Promotion of Science, Grant-in-Aid for JSPS Fellows, Hokkaido University, 16J02256

道南SDGs action
Oct. 2022
株式会社北海道新聞