SEARCH
Search DetailsWakeman Kevin
| Faculty of Science Biological Sciences Biodiversity | Assistant Professor |
My research interests broadly revolve around exploring the biodiversity and evolution single-celled marine microeukaryotes (protists). Currently, I am working on alveolates, a collective of organisms that include notorious apicompelxan pathogens such as causative agent of malaria, as well as dinoflagellate microalgae that produce harmful algal blooms (red tides) and shellfish poisoning. My goal is to apply this work to ecological and economical significant systems such as coral reef biology and shellfish fisheries in the oceans around Japan and globally.
Researcher basic information
■ Degree■ URL
researchmap URLホームページURL■ Various IDs
J-Global ID■ Research Keywords and Fields
Research KeywordResearch Field
- Life Science, Biodiversity and systematics
- Life Science, Evolutionary biology
- Life Science, Morphology and anatomical structure
- Life Science, Cell biology
- Life Science, Molecular biology
- Master's degree program, Graduate School of Science
- Doctoral (PhD) degree program, Graduate School of Science
Career
■ CareerCareer
- Mar. 2016 - Present
Institute for the Advancement of Higher Education (ISP)/Graduate School of Science, Hokkaido University, Biology, Assistant Professor - Apr. 2015 - Feb. 2016
University of Tokyo, Center for Global Communication Strategies, Assistant Professor - Jan. 2013 - Mar. 2015
Okinawa Institute of Science and Technology, Researcher/Research Specialist - Jun. 2010 - Dec. 2013
University of British Coloumbia, Zoology Department, Teaching Assistant
Research activity information
■ Awards- Apr. 2019, Hokkaido University, Young Researcher Award
Kevin Wakeman - 2013, University of British Columbia, Zoology Department, Zoology Graduate Student Award
Kevin Wakeman
- Taxonomic re-evaluation of the Braarudosphaera bigelowii species complex (Prymnesiophyceae)
Kyoko Hagino; Yoshihito Takano; Kevin C. Wakeman; Takeo Horiguchi; Naotaka Tomioka; Ken-ichiro Ishida; Chinatsu Tsukakoshi; Atsushi Nakamura; Kendra Turk-Kubo; Ana María Cabello; Siratee Riewluang; Aika Yamaguchi; Katsunori Kimoto; Masahide Wakita; Masanobu Kawachi; Mary-Hélène Noël; Noriaki Ozaki; Tomohiro Nishimura; Masao Adachi; Keizo Nagasaki; Jonathan P. Zehr
Phycologia, 04 May 2026
Scientific journal - Diversity of convolutid acoels (Convolutidae, Acoela) in Hokkaido, Japan, including a description of the symbiont-bearing Amphiscolops ranshimensis n. sp.
Siratee Riewluang; Regine C. Manglicmot; Shaun Cunningham; Matthew D. Hooge; Rintaro Ono; Hiroshi Kajihara; Brian S. Leander; Kevin C. Wakeman
Zoologischer Anzeiger, 322, 96, 109, Elsevier BV, May 2026, [Peer-reviewed], [Internationally co-authored], [International Magazine]
English, Scientific journal - Diversity and toxicity of Coolia from the Ryukyu Islands, with a description of Coolia kabiraensis sp. nov
Shaun Cunningham; Siratee Riewluang; Aika Yamaguchi; Anne Rolton; Kevin C. Wakeman
HARMFUL ALGAE, 154, 103075, 103075, Apr. 2026, [International Magazine]
English, Scientific journal - Global diversity and distribution of coral-associated protists
Javier del Campo; Anthony M. Bonacolta; Bradley A. Weiler; Ben Knowles; Amy Apprill; Michael D. Fox; Kevin C. Wakeman; Mark J. A. Vermeij; Forest Rohwer; Patrick J. Keeling
22 Jan. 2026 - Multiple independent origins of asexuality and polyploidy among cryptic species of the brown alga Scytosiphon promiscuus (Scytosiphonaceae, Ectocarpales)
Masakazu Hoshino; Masanori Hiraoka; Kevin C. Wakeman; Shimpei F. Hiruta; Kazuhiro Kogame
PHYCOLOGICAL RESEARCH, 74, 1, 8, 21, Jan. 2026
English, Scientific journal - A Practical Approach to Study Uncultivated Protists Using Single-Cell Techniques for Electron Microscopy.
Maia V Palka; Kevin C Wakeman; Eunji Park; Gordon Lax; Brian S Leander
The Journal of eukaryotic microbiology, 73, 2, e70061, 2026, [International Magazine]
English, Scientific journal, Protists represent a significant portion of eukaryotic diversity with a wide range of ecological roles, lifestyles, and diverse morphological traits. Despite their widespread importance in ecological systems and their potential as model organisms, most protist lineages remain poorly characterized, in part due to their small size and the fact that many lineages remain uncultivated. High-resolution microscopy techniques like transmission and scanning electron microscopy (TEM and SEM) are powerful tools for studying protists, but the application of these techniques has significant limitations when applied to uncultivated species, largely due to the absence of reproducible methodologies tailored to studying single cells with small sample sizes. We present a robust protocol for preparing individual eukaryotic cells for TEM and SEM that addresses these limitations, can be implemented in the field, and uses inexpensive and easily obtainable materials. Our method minimizes cell loss during sample preparation for TEM and SEM, and it enables the tracking of single cells through TEM preparation to ensure cells can be localized and oriented appropriately for ultramicrotome sectioning. This protocol expands the feasibility of ultrastructural studies on uncultivated protist lineages and aims to make high-resolution microscopy more accessible for the broader community of protistologists who study uncultivated taxa. - High genetic diversity of Labyrinthula spp. inhabiting mangrove leaf detritus in Okinawa, Japan
Natalia C. Arana; Siratee Riewluang; Fernando Arteaga Arteaga; Marcel Pascal Beier; Kevin C. Wakeman
MARINE BIOLOGY, 173, 1, 02 Dec. 2025
English, Scientific journal - Non-photosynthetic Plastid Replacement by a Primary Plastid in the Making
Yong Heng Phua; Dewi Langlet; Bruno M. Humbel; Arno Hagenbeek; Vasyl Vaskivskyi; Kevin Wakeman; Filip Husnik
08 Oct. 2025 - Molecular phylogeny of Tetraselmis with a description of Tetraselmis convallis sp. nov. (Chlorodendrophyceae, Chlorophyta) isolated from a convolutid acoel (Convolutidae)
Siratee Riewluang; Kevin C. Wakeman
PHYCOLOGIA, 64, 4, 311, 321, 04 Jul. 2025
English, Scientific journal - Divergent Plastid Genomes in the Deepest-Branching Apicomplexan Parasites.
Gordon Lax; Waldan K Kwong; Ina Na; Victoria K L Jacko-Reynolds; Morelia Trznadel; Chloe S E House; Varsha Mathur; Kevin C Wakeman; Patrick J Keeling
Genome biology and evolution, 17, 6, 30 May 2025, [International Magazine]
English, Scientific journal - Comparative Sensitivity of MRI Indices for Myelin Assessment in Spinal Cord Regions.
Philip Kyeremeh Jnr Oppong; Hiroyuki Hamaguchi; Maho Kitagawa; Nina Patzke; Kevin C Wakeman; Khin Khin Tha
Tomography (Ann Arbor, Mich.), 11, 1, 14 Jan. 2025, [International Magazine]
English, Scientific journal - Ultrastructure of Olkasia polycarbonata (Euglenozoa, Euglenida) demonstrates cytoskeletal innovations associated with the feeding and flagellar apparatuses.
Maia V Palka; Regine Claire Manglicmot; Gordon Lax; Kevin C Wakeman; Brian S Leander
The Journal of eukaryotic microbiology, 72, 1, e13074, 2025, [International Magazine]
English, Scientific journal - Phylogenomic diversity of archigregarine apicomplexans.
Gordon Lax; Eunji Park; Ina Na; Victoria Jacko-Reynolds; Waldan K Kwong; Chloe S E House; Morelia Trznadel; Kevin Wakeman; Brian S Leander; Patrick Keeling
Open biology, 14, 9, 240141, 240141, Sep. 2024, [International Magazine]
English, Scientific journal - Marine microturbellarians from Japan, with descriptions of two new species of Reinhardorhynchus (Platyhelminthes, Rhabdocoela, Koinocystididae)
Aoi Tsuyuki; Jhoe Reyes; Yuki Oya; Kevin C. Wakeman; Brian S. Leander; Niels W. L. Van Steenkiste
ZOOSYSTEMATICS AND EVOLUTION, 100, 3, 877, 895, 03 Jul. 2024
English, Scientific journal - Taxonomic study of the polyphyletic Dudresnaya (Dumontiaceae, Florideophyceae) with descriptions of Dudresnaya ryukyuensis sp. nov. and two new genera, Himehibirhodia and Nudresdaya
Masakazu Hoshino; Kevin C. Wakeman; Aki Kato; Taiju Kitayama; Alison R. Sherwood; Shinya Uwai; Kazuhiro Kogame
PHYCOLOGICAL RESEARCH, 72, 2, 133, 148, Apr. 2024
English, Scientific journal - GINSA: an accumulator for paired locality and next-generation small ribosomal subunit sequence data.
Eric Odle; Samuel Kahng; Siratee Riewluang; Kyoko Kurihara; Kevin C Wakeman
Bioinformatics (Oxford, England), 40, 4, 29 Mar. 2024, [International Magazine]
English, Scientific journal - Parallel functional reduction in the mitochondria of apicomplexan parasites.
Patrick J Keeling; Mahara Mtawali; Morelia Trznadel; Samuel J Livingston; Kevin C Wakeman
European journal of protistology, 94, 126065, 126065, 09 Mar. 2024, [International Magazine]
English, Scientific journal - Pacific marine gregarines (Apicomplexa) shed light on biogeographic speciation patterns and novel diversity among early apicomplexans
Eric Odle; Siratee Riewluang; Kentaro Ageishi; Hiroshi Kajihara; Kevin C. Wakeman
European Journal of Protistology, 94, 126080, 126080, Mar. 2024, [International Magazine]
English, Scientific journal - Coral geometry and why it matters.
Samuel E Kahng; Eric Odle; Kevin C Wakeman
PeerJ, 12, e17037, 2024, [International Magazine]
English, Scientific journal - Patterns of host-parasite associations between marine meiofaunal flatworms (Platyhelminthes) and rhytidocystids (Apicomplexa).
Niels W L Van Steenkiste; Kevin C Wakeman; Bill Söderström; Brian S Leander
Scientific reports, 13, 1, 21050, 21050, 29 Nov. 2023, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
English, Scientific journal - Phylogenomics shows that novel tapeworm-like traits of haplozoan parasites evolved from within the Peridiniales (Dinoflagellata).
Eunji Park; Elizabeth Cooney; Yong Heng Phua; Takeo Horiguchi; Filip Husnik; Patrick Keeling; Kevin Wakeman; Brian Leander
Molecular phylogenetics and evolution, 186, 107859, 107859, Sep. 2023, [International Magazine]
English, Scientific journal - Bacterial communities and toxin profiles of Ostreopsis (Dinophyceae) from the Pacific island of Okinawa, Japan.
Yong Heng Phua; Javier Tejeda; Michael C Roy; Filip Husnik; Kevin C Wakeman
European journal of protistology, 89, 125976, 125976, 30 Mar. 2023, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal - Reconstruction of plastid proteomes of apicomplexans and close relatives reveals the major evolutionary outcomes of cryptic plastids.
Varsha Mathur; Eric D Salomaki; Kevin C Wakeman; Ina Na; Waldan K Kwong; Martin Kolisko; Patrick J Keeling
Molecular biology and evolution, 40, 1, 05 Jan. 2023, [International Magazine]
English, Scientific journal - EXPRESSION PATTERNS OF THE THREE AROMATASE ISOFORMS AND EFFECT OF EDITING CONCEPTUS CYP19A2 EXPRESSION ON MAINTENANCE OF PREGNANCY IN THE PIG
Kevin Wakeman
MOLECULAR REPRODUCTION AND DEVELOPMENT, 2023
Scientific journal - Biodiversity of symbiotic microalgae associated with meiofaunal marine acoels in Southern Japan.
Siratee Riewluang; Kevin C Wakeman
PeerJ, 11, e16078, 2023, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal - Morphology and molecular phylogeny of the benthic dinoflagellates (Dinophyceae, Peridiniales) Amphidiniopsis crumena n. sp. and Amphidiniopsis nileribanjensis n. sp.
Aika Yamaguchi; Mona Hoppenrath; Shauna Murray; Anna Liza Kretzschmar; Takeo Horiguchi; Kevin C Wakeman
European journal of protistology, 87, 125940, 125940, 07 Nov. 2022, [Last author], [International Magazine]
English, Scientific journal - Phylogeny of Amphidinium (Dinophyceae) from Guam and Okinawa, with descriptions of A. pagoense sp. nov. and A. uduigamense sp. nov.
Yong Heng Phua; Filip Husnik; Sarah Lemer; Kevin C. Wakeman
PHYCOLOGIA, Apr. 2022, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal - Description of an Enigmatic Alveolate, Platyproteum noduliferae n. sp., and Reconstruction of its Flagellar Apparatus.
Koh Yokouchi; Davis Iritani; Kay Hian Lim; Yong Heng Phua; Takeo Horiguchi; Kevin C Wakeman
Protist, 173, 3, 125878, 125878, 01 Apr. 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Platyproteum is an enigmatic, monotypic genus formerly assigned to the Apicomplexa, until a recent phylogenomic study demonstrated that it diverged from the base of the chromerid/colpodellid (chrompodellid) taxa and apicomplexan clade. In the present study, a new species, P. noduliferae n. sp., is described using a combination of morphological and molecular data. Moreover, a reconstruction of the flagellar apparatus is presented to characterize the presence of flagella which was, until this study, an unknown trait for this genus. Phylogenetic analyses using rDNA sequences suggested that P. noduliferae n. sp. is a sister species of P. vivax, diverging from the base of chrompodellids and apicomplexans. This study provides new morphological data that corroborates the position of Platyproteum amongst other biflagellate species, contributing to an improved understanding of Platyproteum and the evolutionary changes undergone by some marine alveolates as they transitioned into obligate parasitic life styles. - Diversity and toxicity of Pacific strains of the benthic dinoflagellate Coolia (Dinophyceae), with a look at the Coolia canariensis species complex
Yong Heng Phua; Michael C. Roy; Sarah Lemer; Filip Husnik; Kevin C. Wakeman
Harmful Algae, 109, 102120, 102120, Elsevier BV, Oct. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Coolia Meunier 1919 from benthic assemblages of Hawai'i and Guam were isolated and clonal cultures were established from single cells. Cultures were identified to species-level based on 28S rRNA and ITS-5.8S rRNA genes and tested for toxicity. In Hawai'i, two strains of C. malayensis were isolated. In Guam, a high biodiversity was identified: four strains of C. malayensis, one strain of C. palmyrensis, one strain of C. tropicalis, one strain of C. canariensis phylogroup III, and two strains forming a new phylogroup (phylogroup IV) of nontoxic C. canariensis. Morphology of the new C. canariensis phylogroup was described using light microscopy and scanning electron microscopy. Mass cultures and methanol extracts of representative cultures (C. malayensis, C. palmyrensis, C. canariensis, C. tropicalis) from Guam were prepared for liquid chromatography-mass spectrometry analysis. Chemical analyses revealed yessotoxin analogue C56H78O18S2 is produced by C. malayensis, C. canariensis phylogroup IV and C. palmyrensis, but other analogues, C57H80O18S2 and C58H86O18S2, were only found in C. malayensis (Okinawa) and C. canariensis phylogroup IV. Individual toxin profiles were also different over time for an Okinawa strain of C. malayensis (NIES-3637), highlighting intra and inter-species variation in Yessotoxin-analogue expression. Biological activity was tested using Artemia bioassay and toxicity was observed in Guam and Okinawa strains of C. malayensis. Strong support of four distinct clades within the C. canariensis species complex was recovered in phylogenetic analyses, despite morphological similarities. - Parallel functional reduction in the mitochondria of apicomplexan parasites.
Varsha Mathur; Kevin C Wakeman; Patrick J Keeling
Current biology : CB, 31, 13, 2920, 2928, 12 Jul. 2021, [Peer-reviewed], [International Magazine]
English, Scientific journal, Gregarines are an early-diverging lineage of apicomplexan parasites that hold many clues into the origin and evolution of the group, a remarkable transition from free-living phototrophic algae into obligate parasites of animals.1 Using single-cell transcriptomics targeting understudied lineages to complement available sequencing data, we characterized the mitochondrial metabolic repertoire across the tree of apicomplexans. In contrast to the large suite of proteins involved in aerobic respiration in well-studied parasites like Toxoplasma or Plasmodium,2 we find that gregarine trophozoites have significantly reduced energy metabolism: most lack respiratory complexes III and IV, and some lack the electron transport chains (ETCs) and tricarboxylic acid (TCA) cycle entirely. Phylogenomic analyses show that these reductions took place several times in parallel, resulting in a functional range from fully aerobic organelles to extremely reduced "mitosomes" restricted to Fe-S cluster biosynthesis. The mitochondrial genome has also been lost repeatedly: in species with severe functional reduction simply by gene loss but in one species with a complete ETC by relocating cox1 to the nuclear genome. Severe functional reduction of mitochondria is generally associated with structural reduction, resulting in small, nondescript mitochondrial-related organelles (MROs).3 By contrast, gregarines retain distinctive mitochondria with tubular cristae, even the most functionally reduced cases that also lack genes associated with cristae formation. Overall, the parallel, severe reduction of gregarine mitochondria expands the diversity of organisms that contain MROs and further emphasizes the role of parallel transitions in apicomplexan evolution. - New gregarine species (Apicomplexa) from tunicates show an evolutionary history of host switching and suggest a problem with the systematics of Lankesteria and Lecudina.
Davis Iritani; Jonathan C Banks; Stephen C Webb; Andrew Fidler; Takeo Horiguchi; Kevin C Wakeman
Journal of invertebrate pathology, 183, 107622, 107622, Jul. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Apicomplexa (sensu stricto) are a diverse group of obligate parasites to a variety of animal species. Gregarines have been the subject of particular interest due to their diversity, phylogenetically basal position, and more recently, their symbiotic relationships with their hosts. In the present study, four new species of marine eugregarines infecting ascidian hosts (Lankesteria kaiteriteriensis sp. nov., L. dolabra sp. nov., L. savignyii sp. nov., and L. pollywoga sp. nov.) were described using a combination of morphological and molecular data. Phylogenetic analysis using small subunit rDNA sequences suggested that gregarines that parasitize ascidians and polychaetes share a common origin as traditionally hypothesized by predecessors in the discipline. However, Lankesteria and Lecudina species did not form clades as expected, but were instead intermixed amongst each other and their respective type species in the phylogeny. These two major genera are therefore taxonomically problematic. We hypothesize that the continued addition of new species from polychaete and tunicate hosts as well as the construction of multigene phylogenies that include type-material will further dissolve the currently accepted distinction between Lankesteria and Lecudina. The species discovered and described in the current study add new phylogenetic and taxonomic data to the knowledge of marine gregarine parasitism in ascidian hosts. - Evidence for Host Jumping and Diversification of Marine Cephaloidophorid Gregarines (Apicomplexa) Between Two Distantly Related Animals, viz., Crustaceans and Salps.
Kevin C Wakeman; Shimpei Hiruta; Yusuke Kondo; Susumu Ohtsuka
Protist, 172, 4, 125822, 125822, 20 Jun. 2021, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
English, Scientific journal, This study examined the evolutionary history and diversity of marine gregarine parasites of pelagic zooplankton, and highlighted a unique example of a host-jumping event of cephaloidophorid gregarines between two distantly related host groups, crustaceans and chordates. Candacia bipinnata Giesbrecht, 1889, a free-living calanoid copepod, and a salp, Salpa fusiformis Cuvier, 1804, were collected on oceanic research cruises in 2018 and 2019, in the West Pacific aboard TRV SEISUI MARU and TOYOSHIO MARU, respectively. A molecular phylogeny based on 18S rDNA nested the gregarine parasite from S. fusiformis among cephaloidophorids, within a clade exclusively comprised of gregarines from crustaceans. The relationship between these groups was underpinned with ultrastructural data including the presence of a septum, and similarities in the apices of the epicytic folds. Subsequently, it was concluded to establish a new combination, Cephaloidophora cf. flava n. comb (Ex. Thalicola flava) and transfer the other two members of the Thalicola (also parasites of salps) to the Cephaloidophora. This study also attempted to ascertain the origin of cephaloidophorids in S. fusiformis. However, the relationship between Cephaloidophora bipinnatae n. sp., and C. cf. flava n. comb. had only modest support. - Grappleria corona gen. et sp. nov. (Platyhelminthes: Rhabdocoela: Jenseniidae fam. nov.) and an updated molecular phylogeny of 'dalyelliid' and temnocephalid microturbellarians
Niels W. L. Van Steenkiste; Natalie Rivlin; Penelope Kahn; Kevin Wakeman; Brian S. Leander
SYSTEMATICS AND BIODIVERSITY, 19, 3, 261, 272, Apr. 2021, [Peer-reviewed]
English, Scientific journal - A non-functional copy of the salmonid sex determining gene (sdY) is responsible for the "apparent" XY females in Chinook salmon,
Oncorhynchus tshawytscha .
Kevin Wakeman
bioRxiv, 2021
Scientific journal - A new record of the marine chaetonotid gastrotrich genus Diuronotus (Paucitubulatina: Muselliferidae) from the Sea of Japan
Niels W. L. Van Steenkiste; Kevin C. Wakeman; Brian S. Leander
ZOOLOGISCHER ANZEIGER, 290, 113, 116, Jan. 2021, [Peer-reviewed]
English, Scientific journal - Molecular phylogeny and ultrastructure of two novel parasitic dinoflagellates,Haplozoon gracile sp. nov. and H. pugnus sp. nov.
Mana Yamamoto; Kevin C. Wakeman; Shinri Tomioka; Takeo Horiguchi
PHYCOLOGIA, 59, 4, 305, 319, Jul. 2020, [Peer-reviewed]
English, Scientific journal - Molecular Phylogeny of Marine Gregarines (Apicomplexa) from the Sea of Japan and the Northwest Pacific Including the Description of Three Novel Species of Selenidium and Trollidium akkeshiense n. gen. n. sp.
Kevin C Wakeman
Protist, 171, 1, 125710, 125710, Feb. 2020, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
English, Scientific journal, This study set out to bolster morphological and molecular datasets of marine gregarine apicomplexans. Gregarines were sampled from the Sea of Japan and Northwest Pacific from cirratuliform polychaetes (Acrocirridae, Cirratulidae, and Flabelligeridae), as well as sipunculids. Trophozoites (feeding stages) were gathered for identification using light microscopy, scanning electron microscopy, and transmission electron microscopy. Cells were also collected for molecular phylogenetic analysis using 18S rDNA and 28S rDNA. As a result, three new species of Selenidium, S. planusae n. sp., S. validusae n. sp., and S. pyroidea n. sp. were described, and additional morphological and genetic data were gathered for an existing species, S. orientale; and Trollidium was established as a new genus. Trollidium akkeshiense n. gen. n. sp. possessed a unique, unsymmetrical organization of microtubules running the longitudinal length of one side of the trophozoite, corresponding to a zig-zag pattern of epicytic (surface) folds, and a flicking pattern of movement. Phylogenetic analyses of 18S rDNA and 28S rDNA showed that these portions of the ribosomal operon are able to resolve some relationships among Selenidium, while other lineages including Trollidium akkeshiense n. gen. n. sp. appeared to be highly influenced by long branch attraction. High evolutionary rates along the ribosomal operon of gregarines may hinder this marker from resolving deeper nodes among early apicomplexans. - Cosmopolitan Distribution of Endozoicomonas-Like Organisms and Other Intracellular Microcolonies of Bacteria Causing Infection in Marine Mollusks.
Irene Cano; David Ryder; Steve C Webb; Brian J Jones; Cara L Brosnahan; Noelia Carrasco; Barbara Bodinier; Dolors Furones; Tobia Pretto; Francesca Carella; Bruno Chollet; Isabelle Arzul; Deborah Cheslett; Evelyn Collins; Karin B Lohrmann; Ana L Valdivia; Georgia Ward; María J Carballal; Antonio Villalba; Ionan Marigómez; Stein Mortensen; Kevin Christison; Wakeman C Kevin; Eduardo Bustos; Lyndsay Christie; Matthew Green; Stephen W Feist
Frontiers in microbiology, 11, 577481, 577481, 2020, [Peer-reviewed], [International Magazine]
English, Scientific journal, Intracellular microcolonies of bacteria (IMC), in some cases developing large extracellular cysts (bacterial aggregates), infecting primarily gill and digestive gland, have been historically reported in a wide diversity of economically important mollusk species worldwide, sometimes associated with severe lesions and mass mortality events. As an effort to characterize those organisms, traditionally named as Rickettsia or Chlamydia-like organisms, 1950 specimens comprising 22 mollusk species were collected over 10 countries and after histology examination, a selection of 99 samples involving 20 species were subjected to 16S rRNA gene amplicon sequencing. Phylogenetic analysis showed Endozoicomonadaceae sequences in all the mollusk species analyzed. Geographical differences in the distribution of Operational Taxonomic Units (OTUs) and a particular OTU associated with pathology in king scallop (OTU_2) were observed. The presence of Endozoicomonadaceae sequences in the IMC was visually confirmed by in situ hybridization (ISH) in eight selected samples. Sequencing data also indicated other symbiotic bacteria. Subsequent phylogenetic analysis of those OTUs revealed a novel microbial diversity associated with molluskan IMC infection distributed among different taxa, including the phylum Spirochetes, the families Anaplasmataceae and Simkaniaceae, the genera Mycoplasma and Francisella, and sulfur-oxidizing endosymbionts. Sequences like Francisella halioticida/philomiragia and Candidatus Brownia rhizoecola were also obtained, however, in the absence of ISH studies, the association between those organisms and the IMCs were not confirmed. The sequences identified in this study will allow for further molecular characterization of the microbial community associated with IMC infection in marine mollusks and their correlation with severity of the lesions to clarify their role as endosymbionts, commensals or true pathogens. - Validation of a universal set of primers to study animal-associated microeukaryotic communities
Javier del Campo; Maria J. Pons; Maria Herranz; Kevin C. Wakeman; Juana del Valle; Mark J. A. Vermeij; Brian S. Leander; Patrick J. Keeling
ENVIRONMENTAL MICROBIOLOGY, 21, 10, 3855, 3861, Oct. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal - Morphological and molecular diversity of rissoellids (Mollusca, Gastropoda, Heterobranchia) from the Northwest Pacific island of Hokkaido, Japan
Siaden Luis E. Chira; Wakeman Kevin C; Webb Stephen C; Hasegawa Kazunori; Kajihara Hiroshi
ZOOTAXA, 4551, 4, 415, 431, 04 Feb. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal - PCR and histology identify new bivalve hosts of Apicomplexan-X (APX), a common parasite of the New Zealand flat oyster Ostrea chilensis.
Suong NT; Banks JC; Fidler A; Jeffs A; Wakeman KC; Webb S
Diseases of aquatic organisms, 132, 3, 181, 189, Jan. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, Apicomplexan-X (APX) is a significant pathogen of the flat oyster Ostrea chilensis in New Zealand. The life cycle and host range of this species are poorly known, with only the zoite stage identified. Here, we report the use of molecular approaches and histology to confirm the presence of APX in samples of green-lipped mussels Perna canaliculus, Mediterranean mussels Mytilus galloprovincialis and hairy mussels Modiolus areolatus collected from widely distributed locations in New Zealand. The prevalence of APX infection estimated by PCR was 22.2% (n = 99) and 50% (n = 30) in cultured green-lipped mussels from Nelson and Coromandel, respectively; 0.8% (n = 258), 3.3% (n = 150) and 35.3% (n = 17) in wild Mediterranean mussels from Nelson, Foveaux Strait and Golden Bay, respectively; and 46.7% (n = 30) in wild hairy mussels from Foveaux Strait. Histology detected all cases of PCR that were positive with APX and appeared to be more sensitive. The prevalence of APX estimated by histology in green-lipped mussels from Coromandel was 60% versus 50% by PCR, and 4.3%, 10.7% and 52.9% by histology versus 0.8%, 3.3% and 35.3% by PCR in wild Mediterranean mussels from Nelson, Foveaux Strait and Golden Bay, respectively. The specific identity of the parasite found in mussels was determined by sequencing PCR products for a portion (676 bp) of the 18S rRNA gene; the resulting sequences were 99-100% similar to APX found in flat oysters. Phylogenetic analyses also confirmed that all isolates from green-lipped, Mediterranean and hairy mussels grouped with APX isolates previously identified from flat oysters. This study indicates the wide geographical distribution of APX and highlights the potentially multi-host specific distribution of the parasite in commercially important bivalve shellfish. - Dinoflagellate nucleus contains an extensive endomembrane network, the nuclear net
Gregory S. Gavelis; Maria Herranz; Kevin C. Wakeman; Christina Ripken; Satoshi Mitarai; Gillian H. Gile; Patrick J. Keeling; Brian S. Leander
SCIENTIFIC REPORTS, 9, 1, 839, 839, Jan. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal - Molecular phylogeny of the benthic dinoflagellate Cabra matta (Dinophyceae) from Okinawa, Japan
Aika Yamaguchi; Kevin C. Wakeman; Mona Hoppenrath; Takeo Horiguchi; Hiroshi Kawai
Phycologia, 57, 6, 630, 640, Sep. 2018, [Peer-reviewed]
Scientific journal - PCR test to specifically detect the apicomplexan 'X' (APX) parasite found in flat oysters Ostrea chilensis in New Zealand.
Suong NT; Banks JC; Webb SC; Jeffs A; Wakeman KC; Fidler A
Diseases of aquatic organisms, 129, 3, 199, 205, Aug. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Described here is a polymerase chain reaction (PCR) test to detect the apicomplexan-X (APX) parasite of a flat oyster species, Ostrea chilensis, endemic to New Zealand. The test primers target sequences in the in situ hybridisation probes identified to bind specifically to APX 18S rRNA and amplify a 723 bp DNA product. The test did not amplify 18S rRNA gene sequences of other apicomplexan species, including Toxoplasma gondii, Neospora caninum, Selenidium spp., Cephaloidophorida spp., Lecudina spp. and Thiriotia sp. Of 73 flat oysters identified by histology to be infected with APX at different severities, 69 (95%) tested PCR-positive. Failure to amplify an internal control indicated the presence of PCR inhibitors in the 4 PCR-negative samples. The high analytical sensitivity, specificity and speed of the PCR test should make it a useful tool for detecting APX. - Molecular Phylogenetic Positions and Ultrastructure of Marine Gregarines (Apicomplexa) Cuspisella ishikariensis n. gen., n. sp. and Loxomorpha cf. harmothoe from Western Pacific scaleworms (Polynoidae).
Iritani D; Horiguchi T; Wakeman KC
The Journal of eukaryotic microbiology, 65, 5, 637, 647, Jul. 2018, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Marine gregarines are unicellular parasites of invertebrates commonly found infecting the intestine and coelomic spaces of their hosts. Situated at the base of the apicomplexan tree, marine gregarines offer an opportunity to explore the earliest stages of apicomplexan evolution. Classification of marine gregarines is often based on the morphological traits of the conspicuous feeding stages (trophozoites) in combination with host affiliation and molecular phylogenetic data. Morphological characters of other life stages such as the spore are also used to inform taxonomy when such stages can be found. The reconstruction of gregarine evolutionary history is challenging, due to high levels of intraspecific variation of morphological characters combined with relatively few traits that are taxonomically unambiguous. The current study combined morphological data with a phylogenetic analysis of small subunit rDNA sequences to describe and establish a new genus and species (Cuspisella ishikariensis n. gen., n. sp.) of marine gregarine isolated from the intestine of a polynoid host (Lepidonotus helotypus) collected from Hokkaido, Japan. This new species possesses a set of unusual morphological traits including a spiked attachment apparatus and sits on a long branch on the molecular phylogeny. Furthermore, this study establishes a molecular phylogenetic position for Loxomorpha cf. harmothoe, a previously described marine gregarine, and reveals a new group of gregarines that infect polynoid hosts. - Molecular Phylogeny and Morphology of Haplozoon ezoense n. sp. (Dinophyceae): A Parasitic Dinoflagellate with Ultrastructural Evidence of Remnant Non-photosynthetic Plastids
Kevin C. Wakeman; Aika Yamaguchi; Takeo Horiguchi
Protist, 169, 3, 333, 350, Elsevier GmbH, 01 Jul. 2018, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
English, Scientific journal - Molecular Phylogeny and Surface Morphology of Thiriotia hyperdolphinae n. sp. and Cephaloidophora oradareae n. sp. (Gregarinasina, Apicomplexa) Isolated from a Deep Sea Oradarea sp. (Amphipoda) in the West Pacific
Kevin C. Wakeman; Akinori Yabuki; Katsunori Fujikura; Ko Tomikawa; Takeo Horiguchi
Journal of Eukaryotic Microbiology, 65, 3, 372, 381, Blackwell Publishing Inc., 01 May 2018, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
English, Scientific journal - Molecular Phylogenetic Positions of Two New Marine Gregarines (Apicomplexa)—Paralecudina anankea n. sp. and Lecudina caspera n. sp.—from the Intestine of Lumbrineris inflata (Polychaeta) Show Patterns of Co-evolution
Davis Iritani; Kevin C. Wakeman; Brian S. Leander
Journal of Eukaryotic Microbiology, 65, 2, 211, 219, Blackwell Publishing Inc., 01 Mar. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal - Ultrastructure of the marine benthic dinoflagellate Plagiodinium belizeanum (Dinophyceae) from the southeast Pacific island of Okinawa, Japan
Kevin C. Wakeman; Mona Hoppenrath; Aika Yamaguchi; Greg S. Gavelis; Brian S. Leander; Hisayoshi Nozaki
Phycologia, 57, 2, 209, 222, 11 Jan. 2018, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal - Partial 18S rRNA sequences of apicomplexan parasite 'X' (APX), associated with flat oysters Ostrea chilensis in New Zealand
Nguyen Thao Suong; Stephen Webb; Jonathan Banks; Kevin C. Wakeman; Henry Lane; Andrew Jeffs; Cara Brosnahan; Brian Jones; Andrew Fidler
DISEASES OF AQUATIC ORGANISMS, 127, 1, 1, 9, Dec. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal - Microbial arms race: Ballistic "nematocysts" in dinoflagellates represent a new extreme in organelle complexity
Gregory S. Gavelis; Kevin C. Wakeman; Urban Tillmann; Christina Ripken; Satoshi Mitarai; Maria Herranz; Suat Oezbek; Thomas Holstein; Patrick J. Keeling; Brian S. Leander
SCIENCE ADVANCES, 3, 3, e1602552, Mar. 2017, [Peer-reviewed]
English, Scientific journal - Morphology and molecular phylogeny of the marine gregarine parasite Selenidium oshoroense n. sp. (Gregarina, Apicomplexa) isolated from a Northwest Pacific Hydroides ezoensis Okuda 1934 (Serpulidae, Polychaeta)
Kevin C. Wakeman; Takeo Horiguchi
Marine Biodiversity, 48, 3, 1, 10, 01 Feb. 2017, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal - Description of Ganymedes yurii sp n. (Ganymedidae), a New Gregarine Species from the Antarctic Amphipod Gondogeneia sp (Crustacea)
Andrei Diakin; Kevin C. Wakeman; Andrea Valigurova
JOURNAL OF EUKARYOTIC MICROBIOLOGY, 64, 1, 56, 66, Jan. 2017, [Peer-reviewed]
English, Scientific journal - Description of Ganymedes yurii sp. n. (Ganymedidae), a New Gregarine Species from the Antarctic Amphipod Gondogeneia sp. (Crustacea) (vol 64, pg 56, 2017)
Kevin Wakeman
JOURNAL OF EUKARYOTIC MICROBIOLOGY, 2017
Scientific journal - Molecular Phylogeny of the Benthic Dinoflagellate Genus Amphidiniopsis and its Relationships with the Family Protoperidiniaceae
Aika Yamaguchi; Sadaaki Yoshimatsu; Mona Hoppenrath; Kevin C. Wakeman; Hiroshi Kawai
PROTIST, 167, 6, 568, 583, Dec. 2016, [Peer-reviewed]
English, Scientific journal - Molecular Phylogeny of the Marine Planktonic Dinoflagellate Oxytoxum and Corythodinium (Peridiniales, Dinophyceae)
Fernando Gomez; Kevin C. Wakeman; Aika Yamaguchi; Hisayoshi Nozaki
ACTA PROTOZOOLOGICA, 55, 4, 239, 248, 2016, [Peer-reviewed]
English, Scientific journal - Molecular systematics of marine gregarine apicomplexans from Pacific tunicates, with descriptions of five novel species of Lankesteria
Sonja Rueckert; Kevin C. Wakeman; Holger Jenke-Kodama; Brian S. Leander
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 65, 8, 2598, 2614, Aug. 2015, [Peer-reviewed]
English, Scientific journal - Morphology, phylogeny and novel chemical compounds from Coolia malayensis (Dinophyceae) from Okinawa, Japan
Kevin C. Wakeman; Aika Yamaguchi; Michael C. Roy; Holger Jenke-Kodama
HARMFUL ALGAE, 44, 8, 19, Apr. 2015, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal - Comparative Ultrastructure and Molecular Phylogeny of Selenidium melongena n. sp and S. terebellae Ray 1930 Demonstrate Niche Partitioning in Marine Gregarine Parasites (Apicomplexa)
Kevin C. Wakeman; Matthew B. Heintzelman; Brian S. Leander
PROTIST, 165, 4, 493, 511, Aug. 2014, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal - Molecular Phylogeny and Ultrastructure of Caliculium glossobalani n. gen. et sp (Apicomplexa) from a Pacific Glossobalanus minutus (Hemichordata) Confounds the Relationships Between Marine and Terrestrial Gregarines
Kevin C. Wakeman; James D. Reimer; Holger Jenke-Kodama; Brian S. Leander
JOURNAL OF EUKARYOTIC MICROBIOLOGY, 61, 4, 343, 353, Jul. 2014, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal - Molecular Phylogeny of Marine Gregarine Parasites (Apicomplexa) from Tube-forming Polychaetes (Sabellariidae, Cirratulidae and Serpulidae), Including Descriptions of Two New Species of Selenidium
Kevin C. Wakeman; Brian S. Leander
JOURNAL OF EUKARYOTIC MICROBIOLOGY, 60, 5, 514, 525, Sep. 2013, [Peer-reviewed]
English, Scientific journal - Identity of environmental DNA sequences using descriptions of four novel marine gregarine parasites, Polyplicarium n. gen. (Apicomplexa), from capitellid polychaetes
Kevin C. Wakeman; Brian S. Leander
MARINE BIODIVERSITY, 43, 2, 133, 147, Jun. 2013, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal - Discovery of a Diverse Clade of Gregarine Apicomplexans (Apicomplexa: Eugregarinorida) from Pacific Eunicid and Onuphid Polychaetes, Including Descriptions of Paralecudina n. gen., Trichotokara japonica n. sp., and T. eunicae n. sp.
Sonja Rueckert; Kevin C. Wakeman; Brian S. Leander
JOURNAL OF EUKARYOTIC MICROBIOLOGY, 60, 2, 121, 136, Mar. 2013, [Peer-reviewed]
English, Scientific journal - Molecular Phylogeny of Pacific Archigregarines (Apicomplexa), Including Descriptions of Veloxidium leptosynaptae n. gen., n. sp., from the Sea Cucumber Leptosynapta clarki (Echinodermata), and Two New Species of Selenidium
Kevin C. Wakeman; Brian S. Leander
JOURNAL OF EUKARYOTIC MICROBIOLOGY, 59, 3, 232, 245, May 2012, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal
- 円石藻Braarudosphaera bigelowiiの分類学的再検討
萩野恭子; 高野義人; ZEHR Jonathan; WAKEMAN Kevin; 堀口健雄; 富岡尚敬; 西村朋宏; 足立真佐雄; 脇田昌英; 木元克典, 藻類, 71, 1, 2023 - Species discovery, systematics, and taxonomy of marine gregarines (Apicomplexa)
IRITANI Davis; YOKUCHI Koh; HORIGUCHI Takeo; WAKEMAN Kevin C., 藻類, 68, 1, 2020 - Species discovery and evolution of marine gregarines (Apicomplexa): the ancient relatives of Malaria
IRITANI Naoki Davis; WAKEMAN Kevin C.; HORIGUCHI Takeo, 藻類, 66, 1, 2018 - Taxonomic study of two new Amphidinium species (Dinophyceae) with predominant non-motile stages
KIEFFER E SILVA Joao Henriques; WAKEMAN Kevin C.; TERADA Ryuta; HORIGUCHI Takeo, 藻類, 66, 1, 2018 - 厚岸産寄生性渦鞭毛藻Haplozoonの一種の分類学的研究
山本茉奈; WAKEMAN Kevin; 冨岡森理; 堀口健雄, 藻類, 66, 1, 2018
- A taxonomic study of a novel Haplozoon parasite from Akkeshi, Japan
Mana Yamamoto; Kevin Wakeman; 冨岡森理; 堀口健雄
Japanese Society of Phycology, 20 Mar. 2018, Poster presentation - Taxonomic study of two new Amphidinium species (Dynophyceae) with predominant non-motile stage
Joao Henriques; Kiefer e Silva; Kevin Wakeman; Ryuta Terada; Takeo Horiguchi
Japanese Society of Phycology, 20 Mar. 2018, English, Poster presentation - Diversity of Marine Gregarine Apicomplexans
Davis Iritani; Takeo Horiguchi; Kevin Wakeman
Japanese Society of Phycology, 20 Mar. 2018, English, Oral presentation - The Evolutionary (photosynthetic) history of marine apicomplexans and their bizarre relatives
Kevin Wakeman
Botanical Society of Japan, 10 Nov. 2017, English, Invited oral presentation
[Invited] - Algal toxins from novel strains of benthic dinoflagellates from Okinawa, Japan
Kevin Wakeman
20 Aug. 2015, English, Public discourse
[Invited] - A brief look at remnant plastids in gregarine apicomplexans
Kevin Wakeman
10 Aug. 2015, English, Public discourse
[Invited] - Transciptomics reveal relic plastids and diverse evolutionary histories among marine gregarine apicomplexans
Kevin Wakeman
10 May 2015, English, Public discourse - Apicomplexans in our oceans
Kevin Wakeman
2014, Public discourse
[Invited] - Evolutionary history of two sister species exemplify niche partitioning among gregarine apicomplexans
Kevin Wakeman
International Congress of Protistology, 07 Aug. 2013, English, Oral presentation
[International presentation] - Marine Apicomplexan Associates of Coral Reefs
Kevin Wakeman
Jan. 2013, English, Public discourse
[Invited] - Marine Apicomplexans
Kevin Wakeman
Oct. 2012, English, Public discourse
[Invited] - Evolutionary History of Marine Gregarine Apicomplexans
Kevin Wakeman
International Society of Protistology, 05 Aug. 2012, English, Oral presentation
[International presentation] - Eugregarine and the Archigregarine Concepts
Kevin Wakeman
Joint meeting of the International Society of Evolutionary Protistologsts and the Phycological Society of America, 2011, English, Oral presentation
[International presentation]
- 自然史科学特別講義Ⅲ, 2024年, 修士課程, 理学院
- 自然史科学特別講義Ⅲ, 2024年, 修士課程, 理学院
- 多様性生物学研究法, 2024年, 修士課程, 理学院
- 国際交流Ⅱ, 2024年, 学士課程, 国際本部
- 多様性生物学Ⅰ, 2024年, 学士課程, 総合教育部
- 多様性生物学Ⅱ, 2024年, 学士課程, 総合教育部
- 多様性生物学Ⅲ, 2024年, 学士課程, 理学部
- 専門横断科目(一般科目):自然科学・応用科学, 2024年, 学士課程, 専門横断科目
- 専門横断科目(一般科目):自然科学・応用科学, 2024年, 学士課程, 専門横断科目
- 専門横断科目(一般科目):自然科学・応用科学, 2024年, 学士課程, 専門横断科目
- 専門横断科目(一般科目):自然科学・応用科学, 2024年, 学士課程, 専門横断科目
- 専門横断科目(一般科目):自然科学・応用科学, 2024年, 学士課程, 専門横断科目
- 専門横断科目(一般科目):自然科学・応用科学, 2024年, 学士課程, 専門横断科目
- 科学・技術の世界, 2024年, 学士課程, 全学教育
- 生物学Ⅰ, 2024年, 学士課程, 全学教育
■ Research Themes
- Speciation patterns of Lankesteria (Apicomplexan) parasites and their tunicate (Chordata) hosts
Grants-in-Aid for Scientific Research
01 Apr. 2025 - 31 Mar. 2028
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 25K09752 - Exploring the unknown biodiversity of apicomplexan parasites (Apicomplexa) in bivalve shellfish (Mollusca) in Japan
Grants-in-Aid for Scientific Research
01 Apr. 2020 - 31 Mar. 2023
Wakeman Kevin
For this project, we collected 40 blue mussels, 40 Pacific Oysters and a total of 180 Hokkaido Scallop, from Hokkaido, Japan. Generally, samples were processed for histology, genomic DNA isolation, and also prepared for laser dissection microscopy. We started with the processing of scallop because we had more samples of those. We were able to amplified 18S rDNA and also conduct phylogenetic analyses in order to assess relationships of these parasites compared to samples collected in previous studies around the world. As a results, we found two ciliates, Tichodina pectenis and Ancistrum sp. We also were able to isolate and identify an apicomplexan parasite, Merocystis kathae. This was the first report of all three of these organisms in Japan. For each of these organisms, we were able to gather histology and amplify 18S rDNA. We are currently processing the blue mussels and pacific oysters.
Japan Society for the Promotion of Science, Grant-in-Aid for Early-Career Scientists, Hokkaido University, 20K15856 - Studies on species biodiversity and evolution of enigmatic parasitic dinoflagellates, Haplozoon spp., which acquired multicellularity.
Grants-in-Aid for Scientific Research
01 Apr. 2020 - 31 Mar. 2023
HORIGUCHI TAKEO
The parasitic genus Haplozoon is known to possess multicellular organization and this is the only example of multicellularity within the group Dinophyceae. The aims of this project were to understand species diversity, accurate phylogenetic position and cytological features of the genus Haplozoon. All the specimens from Japan were identified as H. pugnus and also the same species has been identified from Canadian Pacific coast, although the host species were different. Multigene phylogeny based on 241 genes obtained through unicellular transcriptomic method indicated that the group had evolved within the order Peridiniales. Membrane staining study revealed that the organisms have true multicellular organization. Functional and structural analyses on remnant plastid, apical spine and endosymbiotic bacteria are currently underway.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 20H03304 - Cellular identity, systematics and biogeography of apicomplexan parasites Genotype-N and Gemmocystis infecting coral reefs
Grants-in-Aid for Scientific Research
01 Apr. 2018 - 31 Mar. 2021
Wakeman Kevin
This work focused on sampling apicomplexans associated with coral reefs. Samples were collected from Okinawa and in locations around Southern Japan, including Ishigaki, Kagoshima and Hiroshima. Samples were processed for histology and PCR screening of apicomplexans using the 18S rDNA. Samples were also prepared for next generation sequencing, in order to gain a better understanding of the diversity of apicomplexans associated with these samples. In total over 80 species of coral were sampled and sent for sequencing. As a general result, we found that apicomplexans that associate with coral are broadly distributed, geographically and throughout coral reefs. To this end, coral in Japan have similar communities of apicomplexans to those found in other localities such as the Caribbean. While similar, these species are likely different, and appear to be unique to the waters around Japan.
Japan Society for the Promotion of Science, Grant-in-Aid for Early-Career Scientists, Hokkaido University, 18K14774 - Apicomplexans associated with coral reefs in Japan
Grant in-aid for Young Scientists B
Apr. 2018 - Mar. 2020
Kevin Wakeman
Japanese Society for the Promotion of Science (JSPS), Principal investigator, Competitive research funding - Novel haplosporidian and apicomplexan parasites infecting shellfish fisheries between Japan and New Zealand
Bilaterial Joint Research
Apr. 2017 - Mar. 2019
Kevin Wakeman; Jonathan Banks; Stephen Webb
Japanese Society for the Promotion of Science (JSPS), Principal investigator, Competitive research funding
