Researcher basic information

• 博士（生命科学）, 京都大学

• https://researchmap.jp/read0069963?lang=en

• https://orcid.org/0000-0003-1100-7462
• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200901087506211081
• http://www.lowtem.hokudai.ac.jp/plantadapt/ayumi/

• 90546417

• https://orcid.org/0000-0003-1100-7462

• 200901087506211081

• Life Science, Plant molecular biology and physiology

• Master's degree program, Graduate School of Environmental Science
• Doctoral (PhD) degree program, Graduate School of Environmental Science

Research activity information

• Evolutionary origin and photoprotective role of Lhcx in the centric diatom Chaetoceros gracilis
  Minoru Kumazawa; Seiji Akimoto; Atsushi Takabayashi; Ko Imaizumi; Shoko Tsuji; Hazuki Hasegawa; Atsushi Sakurai; Sousuke Imamura; Noriko Ishikawa; Natsuko Inoue-Kashino; Yasuhiro Kashino; Kentaro Ifuku
  Plant Physiology, 04 Aug. 2026
  Scientific journal
• Dynamic regulation of the chloroplast SUFBC2D complex by SUFB expression and CLP protease in Arabidopsis
  Yuting Cheng; Zhaoyang Liu; Bing Yang; Qingsong Jiao; Hisashi Ito; Atsushi Takabayashi; Ryouichi Tanaka; Ting Jia; Xueyun Hu
  Journal of Experimental Botany, 17 Mar. 2026
  Scientific journal
• An efficient clear-native PAGE-based workflow for cryo-electron microscopy sample preparation of large protein complexes.
  Zitong Yang; Shinsa Kameo; Soichiro Seki; Genji Kurisu; Ryouichi Tanaka; Akihiro Kawamoto; Atsushi Takabayashi
  Plant methods, 10 Mar. 2026, [International Magazine]
  English, Scientific journal, BACKGROUND: Cryo-electron microscopy (cryo-EM) has revolutionized protein research by enabling high-resolution structural analysis. However, preparing ultra-large protein complexes (e.g., > 700 kDa) for cryo-EM remains challenging, as it requires preserving both structural integrity and the native state. Conventional isolation methods, such as sucrose density gradient centrifugation, require large sample volumes and provide limited separation resolution. In contrast, native PAGE offers higher resolution; however, no established method exists for extracting protein complexes from gels followed by further purification to achieve high purity. Consequently, no standardized native PAGE-based protocol for cryo-EM sample preparation avoids multiple purification steps. Hence, we aimed to develop a rapid and efficient cryo-EM protein sample preparation method using electroelution with an optimized buffer system that preserves complex integrity to recover target protein complexes after sodium deoxycholate (DOC)-based clear-native PAGE (CN-PAGE). RESULTS: We developed an agarose-acrylamide composite gel, which is simpler to prepare and mechanically more robust than conventional linear-gradient acrylamide gels commonly used for CN-PAGE, facilitating precise band excision for efficient electroelution. Cryo-EM structural analysis of the photosystem I-light-harvesting complex I (PSI-LHCI) supercomplex from Arabidopsis thaliana achieved high resolution (2.18 Å) after electroelution from this gel, requiring only buffer exchange by ultrafiltration to remove DOC before grid preparation, without additional chromatographic purification. This finding suggests that DOC may be the main inhibitor of successful grid preparation. CONCLUSION: Our results demonstrate the potential of this method for isolating large protein complexes from small sample volumes for cryo-EM structural analysis. This approach may expand the scope of cryo-EM targets to include some challenging systems previously hindered by purification difficulties.
• Photosystem II subunit S (PsbS) as a conserved regulator of non-photochemical quenching across green lineage evolution
  Hisashi Dewa; Junko Kishimoto; Ryouichi Tanaka; Atsushi Takabayashi
  Plant and Cell Physiology, 27 Feb. 2026
  Scientific journal
• Structural insights into the divergent evolution of a photosystem I supercomplex in Euglena gracilis
  Takahiro Ishikawa; Kentaro Ifuku; Ryo Nagao; Yoshiki Nakajima; Jian-Ren Shen; Koji Kato; Runa Sakamoto; Minoru Kumazawa; Atsushi Takabayashi
  Science Advances, 31 Oct. 2025
  Scientific journal
• Evolutionary origin and functional mechanism of Lhcx in the diatom photoprotection
  Minoru Kumazawa; Seiji Akimoto; Atsushi Takabayashi; Ko Imaizumi; Shoko Tsuji; Hazuki Hasegawa; Atsushi Sakurai; Sousuke Imamura; Noriko Ishikawa; Natsuko Inoue-Kashino; Yasuhiro Kashino; Kentaro Ifuku
  07 Sep. 2025
• Biochemical and phylogenetic analyses of light-harvesting complexes from Tetraselmis striata
  Yuma N. Yamamoto; Takehiro Suzuki; Yoshifumi Ueno; Tatsuya Tomo; Naoshi Dohmae; Atsushi Takabayashi; Ryo Nagao
  Photosynthesis Research, Jun. 2025, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Abscisic acid enhances non-photochemical quenching through SnRK2 and ABI3 in Physcomitrium patens
  Chang-Hyun Maeng; Takuya Fujita; Junko Kishimoto; Ryouichi Tanaka; Atsushi Takabayashi; Tomomichi Fujita
  Journal of Plant Research, 07 Apr. 2025, [Peer-reviewed]
  English, Scientific journal
• Revisiting the early light-induced protein hypothesis in the sustained thermal dissipation mechanism in yew leaves.
  ZIHAO YE; Sawada M; Iwasa M; Moriyama R; Dey D; Furutani M; Mitsutoshi Kitao; Hara T; Tanaka A; Kishimoto J; Makio Yokono; Seiji Akimoto; Atsushi Takabayashi; Ryouichi Tanaka
  Journal of experimental botany, 01 Jan. 2025, [Peer-reviewed], [Corresponding author]
  English, Scientific journal, Overwintering evergreen trees in boreal regions continuously convert absorbed light energy into heat through a process known as sustained thermal dissipation. To better understand this mechanism, this study examined the alterations in the photosynthetic apparatus and transcriptomes of yew (Taxus cuspidata) leaves throughout the year, comparing sun-exposed and shaded leaves. The Y(II) parameter, conventionally used to estimate the quantum yield of photosystem II (PSII), indicated the occurrence of temperature-dependent thermal dissipation during winter. On the other hand, the levels of photosystem subunits, including the D1 subunit of the PSII reaction center, remained relatively stable year-round, indicating that typical photoinhibition is unlikely to occur. Time-resolved chlorophyll fluorescence analysis revealed that heat dissipation at the PSII antenna is prominent in winter. Winter transcriptomes are notably characterized by a predominance of Elip transcripts encoding early light-induced protein (ELIP), which constitute 20% of the total transcripts, as deduced from RNA-seq analysis. Furthermore, ELIP protein concentration increased to nearly half that of the major light-harvesting complexes. The predicted structure of ELIP includes potential chlorophyll a and carotenoid binding sites. These findings, taken together with a previous report showing ELIP capacity for energy dissipation, lead to a re-evaluation of its significant role in sustained thermal dissipation.
• ArabidopsisSUFBand CLP protease regulate SUFBC2D to manipulate iron-sulfur cluster biosynthesis in chloroplast
  Yuting Cheng; Zhaoyang Liu; Bing Yang; Qingsong Jiao; Hisashi Ito; Atsushi Takabayashi; Ryouichi Tanaka; Ting Jia; Xueyun Hu
  02 Nov. 2024
• Anthocyanins act as a sugar-buffer and an alternative electron sink in response to starch depletion during leaf senescence: a case study on a typical anthocyanic tree species, Acer japonicum.
  Mitsutoshi Kitao; Kenichi Yazaki; Tobita H; Agathokleous E; Kishimoto J; Takabayashi A; Ryouichi Tanaka
  Journal of experimental botany, 01 Jun. 2024, [Peer-reviewed]
  English, Scientific journal, We hypothesized that anthocyanins act as a sugar-buffer and an alternative electron sink during leaf senescence to prevent sugar-mediated early senescence and photoinhibition. To elucidate the role of anthocyanin, we monitored seasonal changes in photosynthetic traits, sugar, starch and N contents, pigment composition, and gene expression profiles in leaves exposed to substantially different light conditions within a canopy of an adult fullmoon maple (Acer japonicum) tree. Enhancement of starch amylolysis accompanied by cessation of starch synthesis occurred in the same manner independent of light conditions. Leaf sugar contents increased, but reached upper limits in the late stage of leaf senescence, even though leaf anthocyanins further increased after complete depletion of starch. Sun-exposed leaves maintained higher energy consumption via electron flow than shade-grown leaves during leaf N resorption. Thus, anthocyanins accumulated in sun-exposed leaves might have a regulative role as a sugar-buffer, retarding leaf senescence, and an indirect photoprotective role as an alternative sink for electron consumption to compensate declines in other metabolic processes such as starch and protein synthesis. In this context, anthocyanins may be key substrates protecting both outer-canopy leaves (against photoinhibition) and inner-canopy leaves (via shading by outer-canopy leaves) from high light stress during N resorption.
• Reversible down-regulation of photosystems I and II leads to fast photosynthesis recovery after long-term drought in Jatropha curcas.
  Helena Sapeta; Makio Yokono; Atsushi Takabayashi; Yoshifumi Ueno; André M Cordeiro; Toshihiko Hara; Ayumi Tanaka; Seiji Akimoto; M Margarida Oliveira; Ryouichi Tanaka
  Journal of experimental botany, 74, 1, 336, 351, 01 Jan. 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Jatropha curcas is a drought-tolerant plant that maintains its photosynthetic pigments under prolonged drought, and quickly regains its photosynthetic capacity when water is available. It has been reported that drought stress leads to increased thermal dissipation in PSII, but that of PSI has been barely investigated, perhaps due to technical limitations in measuring the PSI absolute quantum yield. In this study, we combined biochemical analysis and spectroscopic measurements using an integrating sphere, and verified that the quantum yields of both photosystems are temporarily down-regulated under drought. We found that the decrease in the quantum yield of PSII was accompanied by a decrease in the core complexes of PSII while light-harvesting complexes are maintained under drought. In addition, in drought-treated plants, we observed a decrease in the absolute quantum yield of PSI as compared with the well-watered control, while the amount of PSI did not change, indicating that non-photochemical quenching occurs in PSI. The down-regulation of both photosystems was quickly lifted in a few days upon re-watering. Our results indicate, that in J. curcas under drought, the down-regulation of both PSII and PSI quantum yield protects the photosynthetic machinery from uncontrolled photodamage.
• Exposure to strong irradiance exacerbates photoinhibition and suppresses N resorption during leaf senescence in shade-grown seedlings of fullmoon maple (Acer japonicum).
  Kitao M; Yazaki K; Tobita H; Agathokleous E; Kishimoto J; Takabayashi A; Ryouichi Tanaka
  Frontiers in plant science, 13, Frontiers Media SA, 28 Oct. 2022, [Peer-reviewed]
  English, Scientific journal, Leaves of fullmoon maple (Acer japonicum) turn brilliant red with anthocyanins synthesis in autumn. Based on field observations, autumn coloring mainly occurs in outer-canopy leaves exposed to sun, whereas inner-canopy leaves remain green for a certain longer period before finally turn yellowish red with a smaller amount of anthocyanins. Here, we hypothesized that outer-canopy leaves protect themselves against photooxidative stress via anthocyanins while simultaneously shading inner canopy leaves and protecting them from strong light (holocanopy hypothesis). To test this hypothesis, we investigated photoinhibition and leaf N content during autumn senescence in leaves of pot-grown seedlings of fullmoon maple either raised under shade (L0, ≈13% relative irradiance to open) or transferred to full sunlight conditions on 5^th (LH1), 12^th (LH2), or 18^th (LH3) Oct, 2021. Dry mass-based leaf N (N_mass) in green leaves in shade-grown seedlings was ≈ 30 mg N g^-1 in summer. N_mass in shed leaves (25^th Oct to 1^st Nov) was 11.1, 12.0, 14.6, and 10.1 mg N g^-1 in L0, LH1, LH2, and LH3 conditions, respectively. Higher N_mass was observed in shed leaves in LH2, compared to other experimental conditions, suggesting an incomplete N resorption in LH2. F_v/F_m after an overnight dark-adaptation, measured on 19^th Oct when leaf N was actively resorbed, ranked L0: 0.72 > LH3: 0.56 > LH1: 0.45 > LH2: 0.25. As decreased F_v/F_m indicates photoinhibition, leaves in LH2 condition suffered the most severe photoinhibition. Leaf soluble sugar content decreased, but protein carbonylation increased with decreasing F_v/F_m across shade-grown seedlings (L0, LH1, LH2, and LH3) on 19^th Oct, suggesting impaired photosynthetic carbon gain and possible membrane peroxidation induced by photooxidative stress, especially in LH2 condition with less N resorption efficiency. Although the impairment of N resorption seems to depend on the timing and intensity of strong light exposure, air temperature, and consequently the degree of photoinhibition, the photoprotective role of anthocyanins in outer-canopy leaves of fullmoon maple might also contribute to allow a safe N resorption in inner-canopy leaves by prolonged shading.
• Letter to the Editor: Weak-Acidic Clear-Native Polyacrylamide Gel Electrophoresis for the Separation of the Intact Forms of Thylakoid Protein Complexes
  Renon Matsumae; Shinsa Kameo; Ryouichi Tanaka; Atsushi Takabayashi
  Plant And Cell Physiology, 63, 7, 14 Jul. 2022, [Peer-reviewed], [Last author, Corresponding author]
  English, Scientific journal
• Characterization of photosystem II assembly complexes containing ONE-HELIX PROTEIN1 in Arabidopsis thaliana.
  Hanaki Maeda; Koharu Takahashi; Yoshifumi Ueno; Kei Sakata; Akari Yokoyama; Kozue Yarimizu; Fumiyoshi Myouga; Kazuo Shinozaki; Shin-Ichiro Ozawa; Yuichiro Takahashi; Ayumi Tanaka; Hisashi Ito; Seiji Akimoto; Atsushi Takabayashi; Ryouichi Tanaka
  Journal of plant research, 135, 2, 361, 376, Mar. 2022, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, The assembly process of photosystem II (PSII) requires several auxiliary proteins to form assembly intermediates. In plants, early assembly intermediates comprise D1 and D2 subunits of PSII together with a few auxiliary proteins including at least ONE-HELIX PROTEIN1 (OHP1), OHP2, and HIGH-CHLOROPHYLL FLUORESCENCE 244 (HCF244) proteins. Herein, we report the basic characterization of the assembling intermediates, which we purified from Arabidopsis transgenic plants overexpressing a tagged OHP1 protein and named the OHP1 complexes. We analyzed two major forms of OHP1 complexes by mass spectrometry, which revealed that the complexes consist of OHP1, OHP2, and HCF244 in addition to the PSII subunits D1, D2, and cytochrome b559. Analysis of chlorophyll fluorescence showed that a major form of the complex binds chlorophyll a and carotenoids and performs quenching with a time constant of 420 ps. To identify the localization of the auxiliary proteins, we solubilized thylakoid membranes using a digitonin derivative, glycodiosgenin, and separated them into three fractions by ultracentrifugation, and detected these proteins in the loose pellet containing the stroma lamellae and the grana margins together with two chlorophyll biosynthesis enzymes. The results indicated that chlorophyll biosynthesis and assembly may take place in the same compartments of thylakoid membranes. Inducible suppression of the OHP2 mRNA substantially decreased the OHP2 protein in mature Arabidopsis leaves without a significant reduction in the maximum quantum yield of PSII under low-light conditions, but it compromised the yields under high-light conditions. This implies that the auxiliary protein is required for acclimation to high-light conditions.
• Unique Peripheral Antennas in the Photosystems of the Streptophyte Alga Mesostigma viride.
  Michiki Aso; Renon Matsumae; Ayumi Tanaka; Ryouichi Tanaka; Atsushi Takabayashi
  Plant & cell physiology, 62, 3, 436, 446, 17 Jul. 2021, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Land plants evolved from a single group of streptophyte algae. One of the key factors needed for adaptation to a land environment is the modification in the peripheral antenna systems of photosystems (PSs). Here, the PSs of Mesostigma viride, one of the earliest-branching streptophyte algae, were analyzed to gain insight into their evolution. Isoform sequencing and phylogenetic analyses of light-harvesting complexes (LHCs) revealed that M. viride possesses three algae-specific LHCs, including algae-type LHCA2, LHCA9 and LHCP, while the streptophyte-specific LHCB6 was not identified. These data suggest that the acquisition of LHCB6 and the loss of algae-type LHCs occurred after the M. viride lineage branched off from other streptophytes. Clear-native (CN)-polyacrylamide gel electrophoresis (PAGE) resolved the photosynthetic complexes, including the PSI-PSII megacomplex, PSII-LHCII, two PSI-LHCI-LHCIIs, PSI-LHCI and the LHCII trimer. Results indicated that the higher-molecular weight PSI-LHCI-LHCII likely had more LHCII than the lower-molecular weight one, a unique feature of M. viride PSs. CN-PAGE coupled with mass spectrometry strongly suggested that the LHCP was bound to PSII-LHCII, while the algae-type LHCA2 and LHCA9 were bound to PSI-LHCI, both of which are different from those in land plants. Results of the present study strongly suggest that M. viride PSs possess unique features that were inherited from a common ancestor of streptophyte and chlorophyte algae.
• Substitution of Deoxycholate with the Amphiphilic Polymer Amphipol A8-35 Improves the Stability of Large Protein Complexes during Native Electrophoresis.
  Shinsa Kameo; Michiki Aso; Ryo Furukawa; Renon Matsumae; Makio Yokono; Tomomichi Fujita; Ayumi Tanaka; Ryouichi Tanaka; Atsushi Takabayashi
  Plant & cell physiology, 62, 2, 348, 355, 11 May 2021, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, Native polyacrylamide gel electrophoresis (PAGE) is a powerful technique for protein complex separation that retains both their activity and structure. In photosynthetic research, native-PAGE is particularly useful given that photosynthetic complexes are generally large in size, ranging from 200 kD to 1 MD or more. Recently, it has been reported that the addition of amphipol A8-35 to solubilized protein samples improved protein complex stability. In a previous study, we found that amphipol A8-35 could substitute sodium deoxycholate (DOC), a conventional electrophoretic carrier, in clear-native (CN)-PAGE. In this study, we present the optimization of amphipol-based CN-PAGE. We found that the ratio of amphipol A8-35 to α-dodecyl maltoside, a detergent commonly used to solubilize photosynthetic complexes, was critical for resolving photosynthetic machinery in CN-PAGE. In addition, LHCII dissociation from PSII-LHCII was effectively prevented by amphipol-based CN-PAGE compared with that of DOC-based CN-PAGE. Our data strongly suggest that majority of the PSII-LHCII in vivo forms C2S2M2 at least in Arabidopsis and Physcomitrella. The other forms might appear owing to the dissociation of LHCII from PSII during sample preparation and electrophoresis, which could be prevented by the addition of amphipol A8-35 after solubilization from thylakoid membranes. These results suggest that amphipol-based CN-PAGE may be a better alternative to DOC-based CN-PAGE for the study of labile protein complexes.
• Erratum: Unique Peripheral Antennas in the Photosystems of the Streptophyte Alga Mesostigma Viride (Plant and Cell Physiology (2021) 62:3 (436–446) DOI: 10.1093/pcp/pcaa172)
  Aso, M.; Matsumae, R.; Tanaka, A.; Tanaka, R.; Takabayashi, A.
  Plant and Cell Physiology, 62, 8, 1355, 1355, 2021
  Scientific journal
• Formation of a PSI–PSII megacomplex containing LHCSR and PsbS in the moss Physcomitrella patens
  Ryo Furukawa; Michiki Aso; Tomomichi Fujita; Seiji Akimoto; Ryouichi Tanaka; Ayumi Tanaka; Makio Yokono; Atsushi Takabayashi
  Journal of Plant Research, 132, 6, 867, 880, Springer Science and Business Media {LLC}, Nov. 2019, [Peer-reviewed]
  Scientific journal
• Horizontal Transfer of Promiscuous Activity from Nonphotosynthetic Bacteria Contributed to Evolution of Chlorophyll Degradation Pathway
  Atsushi Takabayashi
  Molecular Biology and Evolution, Aug. 2019, [Peer-reviewed]
  Scientific journal
• The PSI-PSII Megacomplex in Green Plants
  Atsushi Takabayashi
  Plant and Cell Physiology, May 2019, [Peer-reviewed]
  English, Scientific journal
• Regulation of excitation energy in Nannochloropsis photosystem II
  Makio Yokono; Ikumi Umetani; Atushi Takabayashi; Seiji Akimoto; Ayumi Tanaka
  Photosynthesis Research, 1, 7, Springer Netherlands, 27 Apr. 2018, [Peer-reviewed]
  English, Scientific journal
• Evidence of the supercomplex organization of photosystem II and light-harvesting complexes in Nannochloropsis granulata
  Ikumi Umetani; Motoshi Kunugi; Makio Yokono; Atsushi Takabayashi; Ayumi Tanaka
  Photosynthesis Research, 136, 1, 49, 61, Springer Netherlands, 01 Apr. 2018, [Peer-reviewed]
  English, Scientific journal
• Deficiency of the Stroma-Lamellar Protein LIL8/PSB33 Affects Energy Transfer Around PSI in Arabidopsis
  Yukako Kato; Makio Yokono; Seiji Akimoto; Atsushi Takabayashi; Ayumi Tanaka; Ryouichi Tanaka
  PLANT AND CELL PHYSIOLOGY, 58, 11, 2026, 2039, Nov. 2017, [Peer-reviewed]
  English, Scientific journal
• Towards artificial methanogenesis: biosynthesis of the [Fe]-hydrogenase cofactor and characterization of the semisynthetic hydrogenase
  Liping Bai; Takashi Fujishiro; Gangfeng Huang; Urgen Koch; Atsushi Takabayashi; Makio Yokono; Ayumi Tanaka; Tao Xu; Xile Hu; Ulrich Ermler; Seigo Shima
  FARADAY DISCUSSIONS, 198, 37, 58, Jun. 2017, [Peer-reviewed]
  English, Scientific journal
• 光化学系I‐光化学系II超複合体の発見と機能 新しい光防御機構
  横野牧生; 高林厚史; 秋本誠志; 田中歩
  化学と生物, 55, 2, 78‐80, 20 Jan. 2017
  Japanese
• Complete chloroplast genome sequence of the early diverging green alga Palmophyllum crassum
  Ryo Furukawa; Motoshi Kunugi; Kunio Ihara; Atsushi Takabayashi; Ayumi Tanaka
  Genome Announcements, 5, 10, American Society for Microbiology, 2017, [Peer-reviewed]
  English, Scientific journal
• PCoM-DB Update: A Protein Co-Migration Database for Photosynthetic Organisms
  Atsushi Takabayashi; Saeka Takabayashi; Kaori Takahashi; Mai Watanabe; Hiroko Uchida; Akio Murakami; Tomomichi Fujita; Masahiko Ikeuchi; Ayumi Tanaka
  PLANT AND CELL PHYSIOLOGY, 58, 1, Jan. 2017, [Peer-reviewed]
  English, Scientific journal
• NDH-Mediated Cyclic Electron Flow Around Photosystem I is Crucial for C-4 Photosynthesis
  Noriko Ishikawa; Atsushi Takabayashi; Ko Noguchi; Youshi Tazoe; Hiroshi Yamamoto; Susanne von Caemmerer; Fumihiko Sato; Tsuyoshi Endo
  PLANT AND CELL PHYSIOLOGY, 57, 10, 2020, 2028, Oct. 2016, [Peer-reviewed]
  English, Scientific journal
• Accumulation of the components of cyclic electron flow around photosystem I in C-4 plants, with respect to the requirements for ATP
  Noriko Ishikawa; Atsushi Takabayashi; Fumihiko Sato; Tsuyoshi Endo
  PHOTOSYNTHESIS RESEARCH, 129, 3, 261, 277, Sep. 2016, [Peer-reviewed]
  English
• Direct interaction with ACR11 is necessary for post-transcriptional control of GLU1-encoded ferredoxin-dependent glutamate synthase in leaves
  Atsushi Takabayashi; Akihiro Niwata; Ayumi Tanaka
  SCIENTIFIC REPORTS, 6, Jul. 2016, [Peer-reviewed]
  English, Scientific journal
• Evolution of Green Plants Accompanied Changes in Light-Harvesting Systems
  Motoshi Kunugi; Soichirou Satoh; Kunio Ihara; Kensuke Shibata; Yukimasa Yamagishi; Kazuhiro Kogame; Junichi Obokata; Atsushi Takabayashi; Ayumi Tanaka
  PLANT AND CELL PHYSIOLOGY, 57, 6, 1231, 1243, Jun. 2016, [Peer-reviewed]
  English, Scientific journal
• A megacomplex composed of both photosystem reaction centres in higher plants
  M. Yokono; A. Takabayashi; S. Akimoto; A. Tanaka
  NATURE COMMUNICATIONS, 6, Mar. 2015, [Peer-reviewed]
  English, Scientific journal
• Pale-Green Phenotype of atl31 atl6 Double Mutant Leaves Is Caused by Disruption of 5-Aminolevulinic Acid Biosynthesis in Arabidopsis thaliana
  Shugo Maekawa; Atsushi Takabayashi; Thais Huarancca Reyes; Hiroko Yamamoto; Ayumi Tanaka; Takeo Sato; Junji Yamaguchi
  PLOS ONE, 10, 2, Feb. 2015, [Peer-reviewed]
  English, Scientific journal
• Functional Analysis of Light-harvesting-like Protein 3 (LIL3) and Its Light-harvesting Chlorophyll-binding Motif in Arabidopsis
  Kaori Takahashi; Atsushi Takabayashi; Ayumi Tanaka; Ryouichi Tanaka
  JOURNAL OF BIOLOGICAL CHEMISTRY, 289, 2, 987, 999, Jan. 2014, [Peer-reviewed]
  English, Scientific journal
• Evolutionary Changes in Chlorophyllide a Oxygenase (CAO) Structure Contribute to the Acquisition of a New Light-harvesting Complex in Micromonas
  Motoshi Kunugi; Atsushi Takabayashi; Ayumi Tanaka
  JOURNAL OF BIOLOGICAL CHEMISTRY, 288, 27, 19330, 19341, Jul. 2013, [Peer-reviewed]
  English, Scientific journal
• Protein co-migration database (PCoM -DB) for Arabidopsis thylakoids and Synechocystis cells
  Atsushi Takabayashi; Ryosuke Kadoya; Masayoshi Kuwano; Katsunori Kurihara; Hisashi Ito; Ryouichi Tanaka; Ayumi Tanaka
  SPRINGERPLUS, 2, 2013, [Peer-reviewed]
  English, Scientific journal
• Screening of Novel Subunits of Chloroplastic NAD(P)H Dehydrogenase in Arabidopsis
  Ishikawa, N; Takabayashi, A; Ishida, S; Hano, Y; Ifuku, K; Sato F; Endo, T
  In : Kuang, T., Lu, C., Zhang, L. (eds.) Photosynthesis Research for Food, Fuel and the Future, Advanced Topics in Science and Technology in China, Zhejiang Univ. Press, Hangzhou, and Springer-Verlag, Heidelberg., 279, 281, 2013
  English, International conference proceedings
• Chlorophyll metabolism in photosynthetic organisms.
  Tanaka R; Takabayashi A; Ito H; Tanaka A
  Handbook of Porphyrin Science, 20, 213, 242, 2012, [Peer-reviewed], [Invited]
  English, Scientific journal
• The Oligomeric States of the Photosystems and the Light-Harvesting Complexes in the Chl b-Less Mutant
  Atsushi Takabayashi; Katsunori Kurihara; Masayoshi Kuwano; Yasuhiro Kasahara; Ryouichi Tanaka; Ayumi Tanaka
  PLANT AND CELL PHYSIOLOGY, 52, 12, 2103, 2114, Dec. 2011, [Peer-reviewed]
  English, Scientific journal
• Allocation of Absorbed Light Energy in PSII to Thermal Dissipations in the Presence or Absence of PsbS Subunits of Rice
  Satoshi Ishida; Ken-Ichi Morita; Masahiro Kishine; Atsushi Takabayashi; Reiko Murakami; Satomi Takeda; Ko Shimamoto; Fumihiko Sato; Tsuyoshi Endo
  PLANT AND CELL PHYSIOLOGY, 52, 10, 1822, 1831, Oct. 2011, [Peer-reviewed]
  English, Scientific journal
• Identification of the 7-Hydroxymethyl Chlorophyll a Reductase of the Chlorophyll Cycle in Arabidopsis
  Miki Meguro; Hisashi Ito; Atsushi Takabayashi; Ryouichi Tanaka; Ayumi Tanaka
  PLANT CELL, 23, 9, 3442, 3453, Sep. 2011, [Peer-reviewed]
  English, Scientific journal
• LIL3, a light-harvesting-like protein, plays an essential role in chlorophyll and tocopherol biosynthesis
  Ryouichi Tanaka; Maxi Rothbart; Seiko Oka; Atsushi Takabayashi; Kaori Takahashi; Masaru Shibata; Fumiyoshi Myouga; Reiko Motohashi; Kazuo Shinozaki; Bernhard Grimm; Ayumi Tanaka
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 107, 38, 16721, 16725, Sep. 2010, [Peer-reviewed]
  English, Scientific journal
• Three PsbQ-Like Proteins are Required for the Function of the Chloroplast NAD(P)H Dehydrogenase Complex in Arabidopsis
  Shinya Yabuta; Kentaro Ifuku; Atsushi Takabayashi; Seiko Ishihara; Kunio Ido; Noriko Ishikawa; Tsuyoshi Endo; Fumihiko Sato
  PLANT AND CELL PHYSIOLOGY, 51, 6, 866, 876, Jun. 2010, [Peer-reviewed]
  English, Scientific journal
• Direct interaction between KaiA and KaiB revealed by a site-directed spin labeling electron spin resonance analysis
  Risa Mutoh; Hiroyuki Mino; Reiko Murakami; Tatsuya Uzumaki; Atsushi Takabayashi; Kentaro Ishii; Masahiro Ishiura
  GENES TO CELLS, 15, 3, 269, 280, Mar. 2010, [Peer-reviewed]
  English, Scientific journal
• A Novel Nuclear-Encoded Protein, NDH-Dependent Cyclic Electron Flow 5, is Essential for the Accumulation of Chloroplast NAD(P)H Dehydrogenase Complexes
  Satoshi Ishida; Atsushi Takabayashi; Noriko Ishikawa; Yasushi Hano; Tsuyoshi Endo; Fumihiko Sato
  PLANT AND CELL PHYSIOLOGY, 50, 2, 383, 393, Feb. 2009, [Peer-reviewed]
  English, Scientific journal
• Three novel subunits of Arabidopsis chloroplastic NAD(P)H dehydrogenase identified by bioinformatic and reverse genetic approaches
  Atsushi Takabayashi; Noriko Ishikawa; Takeshi Obayashi; Satoshi Ishida; Junichi Obokata; Tsuyoshi Endo; Fumihiko Sato
  PLANT JOURNAL, 57, 2, 207, 219, Jan. 2009, [Peer-reviewed]
  English, Scientific journal
• NDF6: A thylakoid protein specific to terrestrial plants is essential for activity of chloroplastic NAD(P)H dehydrogenase in arabidopsis
  Noriko Ishikawa; Atsushi Takabayashi; Satoshi Ishida; Yasushi Hano; Tsuyoshi Endo; Fumihiko Sato
  PLANT AND CELL PHYSIOLOGY, 49, 7, 1066, 1073, Jul. 2008, [Peer-reviewed]
  English, Scientific journal
• Distinct functions for the two PsbP-like proteins PPL1 and PPL2 in the chloroplast thylakoid lumen of arabidopsis
  Seiko Ishihara; Atsushi Takabayashi; Kunio Ido; Tsuyoshi Endo; Kentaro Ifuku; Fumihiko Sato
  PLANT PHYSIOLOGY, 145, 3, 668, 679, Nov. 2007, [Peer-reviewed]
  English, Scientific journal
• Functional analysis of two PsbP-like (PPL) proteins in Arabidopsis thaliana
  Atsushi Takabayashi
  Photosynthesis Research, 17, 1085, 1088, 2007
  English, Scientific journal
• Screening of novel NDH subunits by comparative genomics
  Takabayashi Atsushi; Endo Tsuyoshi; Sato Fumihiko
  PLANT AND CELL PHYSIOLOGY, 48, S128, 2007, [Peer-reviewed]
• Chloroplastic NAD(P)H dehydrogenase in tobacco leaves functions in alleviation of oxidative damage caused by temperature stress
  P Wang; W Duan; A Takabayashi; T Endo; T Shikanai; JY Ye; HL Mi
  PLANT PHYSIOLOGY, 141, 2, 465, 474, Jun. 2006, [Peer-reviewed]
  English, Scientific journal
• Differential use of two cyclic electron flows around photosystem I for driving CO2-concentration mechanism in C-4 photosynthesis
  A Takabayashi; M Kishine; K Asada; T Endo; F Sato
  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 102, 46, 16898, 16903, Nov. 2005, [Peer-reviewed]
  English, Scientific journal
• Functional dissection of two Arabidopsis PsbO proteins
  R Murakami; K Ifuku; A Takabayashi; T Shikanai; T Endo; F Sato
  FEBS JOURNAL, 272, 9, 2165, 2175, May 2005, [Peer-reviewed]
  English, Scientific journal
• Ribosomal RNA processing and an RNase R family member in chloroplasts of Arabidopsis
  M Kishine; A Takabayashi; Y Munekage; T Shikanai; T Endo; F Sato
  PLANT MOLECULAR BIOLOGY, 55, 4, 595, 606, Jul. 2004, [Peer-reviewed]
  English, Scientific journal
• Post-illumination reduction of the plastoquinone pool in chloroplast transformants in which chloroplastic NAD(P)H dehydrogenase was inactivated
  A Takabayashi; T Endo; T Shikanai; F Sato
  BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 66, 10, 2107, 2111, Oct. 2002, [Peer-reviewed]
  English, Scientific journal
• Characterization of an Arabidopsis thaliana mutant with impaired psbO, one of two genes encoding extrinsic 33-kDa proteins in photosystem II
  R Murakami; K Ifuku; A Takabayashi; T Shikanai; T Endo; F Sato
  FEBS LETTERS, 523, 1-3, 138, 142, Jul. 2002, [Peer-reviewed]
  English, Scientific journal
• The role of chloroplastic NAD(P)H dehydrogenase in photoprotection
  T Endo; T Shikanai; A Takabayashi; K Asada; F Sato
  FEBS LETTERS, 457, 1, 5, 8, Aug. 1999, [Peer-reviewed]
  English, Scientific journal

• 常緑針葉樹4種における冬季の光化学系タンパク質の変動と光化学系の応答
  成田あゆ; 成田あゆ; 小野清美; 岸本純子; 高林厚史; 菅井徹人; 北尾光俊; 田中亮一, 日本森林学会大会学術講演集, 136th, 2025
• ハウチワカエデ紅葉期のアントシアニンの挙動と役割
  北尾光俊; 矢崎健一; 飛田博順; 岸本純子; 高林厚史; 田中亮一, 日本森林学会大会学術講演集, 134th, 2023
• 紅葉は樹冠内部の葉を守り,樹冠全体での炭素獲得と窒素回収に貢献する
  北尾光俊; 矢崎健一; 飛田博順; 高林厚史; 田中亮一, 日本森林学会大会学術講演集, 133rd, 2022
• 常緑針葉樹4種における冬季の光合成色素・光化学系タンパク質の変動
  成田あゆ; 成田あゆ; 小野清美; 高林厚史; 菅井徹人; 北尾光俊; 田中亮一, 日本植物学会大会研究発表記録(CD-ROM), 86th, 2022
• 光化学系の光環境適応とその進化的な制約 -緑藻の光化学系をモデルとして-
  高林 厚史, 光合成研究, Dec. 2017, [Peer-reviewed], [Invited]
  Japanese, Introduction scientific journal
• ブルーネイティブ電気泳動を用いた光合成生物タンパク質複合体の網羅的解析
  高林 厚史; 田中 歩, 電気泳動, 61, 2, 111, 114, Nov. 2017, [Peer-reviewed], [Invited]
  Japanese, Technical report
• ヒメツリガネゴケにおけるアブシジン酸処理時の光合成の変化(ポスター発表,<特集>日本蘚苔類学会第43回青森大会)
  藤田 拓矢; 高林 厚史; 田中 歩; 藤田 知道, 蘚苔類研究, 11, 3, 92, 92, Dec. 2014
  日本蘚苔類学会, Japanese
• コケ植物を用いた宇宙実験に向けて：スペース・モスの活動報告
  藤田知道; 蒲池浩之; 唐原一郎; 久米篤; 坂田洋一; 高林厚史; 田中歩; 長嶋寿江; 西山智明; 橋本博文; 長谷部光泰; 半場祐子; 日渡祐二; 松田修; 本村泰三; 矢野幸子, Proceedings of The Twenty-ninth Space Utilization Sysmposium, 29, 19, 20, 2014
  第29回宇宙環境利用シンポジウム (2015年1月24日-25日. 宇宙航空研究開発機構宇宙科学研究所(JAXA)(ISAS)), 相模原市, 神奈川県
  Space Utilization Research (January 24-25, 2015. Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency(JAXA)(ISAS)), Sagamihara, Kanagawa Japan
  著者人数: 16名
  資料番号: SA6000035009
  レポート番号: ISAS-SUR29-S04, 宇宙航空研究開発機構宇宙科学研究所(JAXA)(ISAS), Japanese
• 3P-218 Genetic modification of plant photosystems: from basic to applied research
  Kunugi Motoshi; Takabayashi Atushi; Tanaka Ayumi, 日本生物工学会大会講演要旨集, 65, 242, 242, 2013
  日本生物工学会, Japanese
• ゲノム世代のタンパク質複合体解析
  光合成研究, 60, 2010
• 地球環境と光合成の共進化
  低温科学, 21, 2010
• 発現プロファイルの利用
  低温科学, 663, 2009
• Functional analysis of two PsbP-like (PPL) proteins in Arabidopsis thaliana
  S. Ishihara; A. Takabayashi; T. Endo; K. Ifuku; F. Sato, PHOTOSYNTHESIS RESEARCH, 91, 2-3, 263, 263, Feb. 2007
  English, Summary international conference
• Functional analysis of PsbP-like (PPL) proteins in Arabidopsis thaliana
  Seiko Ishihara; Atsushi Takabayashi; Tsuyoshi Endo; Kentaro Ifuku; Furnihiko Sato, PLANT AND CELL PHYSIOLOGY, 48, S99, S99, 2007
  English, Summary international conference
• Characterization of chloroplastic RNase E mutants in Arabidopsis
  M Kishine; A Takabayashi; F Sato; T Endo, PLANT AND CELL PHYSIOLOGY, 47, S39, S39, 2006
  English, Summary international conference
• The functional differences of two extrinsic 33-kDa proteins in photosystem II in Arabidopsis thaliana
  R Murakami; K Ifuku; A Takabayashi; T Shikanai; T Endo; F Sato, PLANT AND CELL PHYSIOLOGY, 45, S79, S79, 2004
  English, Summary international conference
• Isolation of chloroplastic RNase mutants in Arabidopsis
  M Kishine; A Takabayashi; T Endo; F Sato, PLANT AND CELL PHYSIOLOGY, 45, S32, S32, 2004
  English, Summary international conference
• Characterization of PsbO and PsbO2 protein in Arabidopsis
  R Murakami; K Ifuku; A Takabayashi; T Shikanai; T Endo; F Sato, PLANT AND CELL PHYSIOLOGY, 44, S190, S190, 2003
  English, Summary international conference
• An RNase R family functions in ribosomal RNA processing in chloroplasts of Arabidopsis
  M Kishine; A Takabayashi; Y Munekage; T Shikanai; F Sato; T Endo, PLANT AND CELL PHYSIOLOGY, 44, S58, S58, 2003
  English, Summary international conference
• A high chlorophyll fluorescence mutant of Arabidopsis has defect in 16S ribosomal RNA maturation
  M Kishine; A Takabayashi; Y Munekage; T Shikanai; F Sato; T Endo, PLANT AND CELL PHYSIOLOGY, 43, S37, S37, 2002
  English, Summary international conference
• Physiological and enzymatic characterization of chloroplastic NAD(P)H dehydrogenase
  A Takabayashi; T Endo; T Shikanai; F Sato, PLANT AND CELL PHYSIOLOGY, 43, S24, S24, 2002
  English, Summary international conference
• Tobacco ndhC-K-J disruptants created by chloroplast transformation and their characterization :
  TAKABAYASHI Atsushi; ENDO Tsuyoshi; SHIKANAI Toshiharu; SATO Fumihiko, Plant and cell physiology, 42, s172, 2001
  Japanese Society of Plant Physiologists, English
• DEFECT IN CHLOROPLASTIC NAD (P) H DEHYDROGENASE COMPLEX RESULTED IN STROMAL OVER-REDUCTION :
  ENDO Tsuyoshi; TAKABAYASHI Atsushi; SHIKANAI Toshiharu; SATO Fumihiko, Plant and cell physiology, 42, s157, 2001
  Japanese Society of Plant Physiologists, English
• CYCLIC ELECTRON TRANSPORT MEDIATED BY CHLOROPLASTIC NAD(P)H DEHYDROGENASE COMPLEX
  ENDO Tsuyoshi; SHIKANAI Toshiharu; TAKABAYASHI Atsushi; SATO Fumihiko, Plant and cell physiology, 40, s36, s36, Mar. 1999
  English

• 環境分子生物学特論Ⅱ, 2024年, 修士課程, 環境科学院
• 分子生物学基礎論, 2024年, 修士課程, 環境科学院
• 分子生物学基礎論, 2024年, 修士課程, 環境科学院
• 一般教育演習(ﾌﾚｯｼｭﾏﾝｾﾐﾅｰ), 2024年, 学士課程, 全学教育

• THE JAPANESE SOCIETY OF PHOTOSYNTHESIS RESEARCH
• 日本植物生理学会

• 海洋から淡水へ ー プラシノ藻類の淡水適応に伴う光化学系の強光適応機構の進化
  科学研究費助成事業
  01 Apr. 2024 - 31 Mar. 2028
  高林 厚史
  日本学術振興会, 基盤研究(C), 北海道大学, 24K09496
• 緑藻メソスティグマのユニークな光防御機構の解析―淡水性緑藻から陸上植物へ
  科学研究費助成事業
  01 Apr. 2021 - 31 Mar. 2024
  高林 厚史
  PsbS遺伝子は緑藻および陸上植物で保存されている遺伝子であり、陸上植物では光合成の熱放散機構に寄与することが明らかになっている。しかし、緑藻のPsbSの機能は未だ明らかではなく、緑藻では（維管束植物には存在しない）LHCSR遺伝子がその生理的機能を担っている。緑藻から維管束植物への進化の過程で熱放散機構がどのように進化したのか、については未解明である。
  
  
  本研究では、緑藻のPsbS遺伝子をシロイヌナズナのPsbS欠損株に導入することで、緑藻のPsbS遺伝子がシロイヌナズナのPsbSの機能を相補できるかどうかを調べることとした。今年度は緑藻とシロイヌナズナのPsbS遺伝子を形質転換ベクターに導入し、シロイヌナズナPsbS欠損株への形質転換を進めている。現在、形質転換体の選抜を進めており、各ラインで十分な数の形質転換体が得られつつある。
  
  
  また、PsbS遺伝子は光化学系IIのLHCIIアンテナに結合すると考えられているため、導入した緑藻のPsbS遺伝子がPSII-LHCII、もしくは、LHCIIアンテナに結合するかどうかを調べる必要がある。そのため、Native-PAGEの改良を進めており、これまでにCN-PAGEのbuffer系を改良することでPSIIからのLHCIIや酸素発生複合体の解離を抑制することが可能になった。形質転換体が得られた後、この系を用いて、導入したPsbSがPSIIやそのLHCアンテナと結合するかどうかを調べる予定である。
  日本学術振興会, 基盤研究(C), 北海道大学, 21K06224
• 葉の窒素代謝ネットワークの転写後制御の解明とその応用
  基盤研究(C)
  2017 - 2019
  高林 厚史
  文部科学省, Principal investigator, Competitive research funding
• Comprehensive study of chlorophyll metabolism based on enzyme identification
  Grants-in-Aid for Scientific Research
  01 Apr. 2015 - 31 Mar. 2018
  TANAKA Ayumi; TAKABAYASHI ATSUSHI; ITO HISASHI
  Despite its importance, Mg-dechelatase that catalyzes the first step of chlorophyll degradation pathway was not identified. In this study, we found that Mg-dechelatase is encoded by SGR gene and the dechelating activity was successfully measured with the recombinant protein expressed in E. coli. We also found that substrate specificity differs between SGR1 and SGRL. By these studies, all major enzymes of the chlorophyll metabolic pathway were finally identified. Furthermore, it was revealed that SGR can catalyze not only free chlorophyll but also chlorophyll bound to protein, thus playing a central role in the degradation of chlorophyll-binding proteins and photosystems.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 15H04381
• 植物の新規窒素代謝制御機構の解明とその応用
  若手研究（B)
  2014 - 2016
  高林 厚史
  文部科学省, Principal investigator, Competitive research funding
• Study on the chlorophyll metabolism from enzymatic, applicable and evolutionary aspects
  Grants-in-Aid for Scientific Research
  01 Apr. 2012 - 31 Mar. 2015
  TANAKA AYUMI; TAKABAYASHI Atsushi
  Chlorophyll metabolism is indispensable for photosynthetic organisms. This metabolic pathway is responsible not only for synthesis and degradation of chlorophyll molecules but also for other physiological processes. In this study, we focus on the following topics: (1) identification of chlorophyll metabolic enzymes; (2) regulation of chlorophyll metabolism; (3) multi-function of chlorophyll metabolism; (4) evolution of chlorophyll metabolism; (5) agricultural application. Especially, we have succeeded in identification of Mg-dechelatase which removes central Mg from chlorophyll. This is the last unidentified enzyme of chlorophyll degradation. How a new metabolic pathway has appeared during evolution is a major biological question. We showed that one of the promiscuous activities of the ancestral enzymes have become the main activity of the present enzyme during evolution. We proposed the importance of enzyme promiscuous activity for acquiring a new metabolic pathway.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 24370017
• 新規手法による葉緑体タンパク質複合体の網羅的検出
  若手研究 (B)
  2011 - 2013
  高林 厚史
  文部科学省, Principal investigator, Competitive research funding
• バイオインフォマティクスの手法を用いたクロロフィル代謝経路の確立
  若手研究 (スタートアップ)→研究活動スタート支援
  2009 - 2010
  高林 厚史
  文部科学省, Principal investigator, Competitive research funding