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MITANI Tomohiro

Research Faculty of Agriculture　Fundamental AgriScience Research　Animal ScienceAssociate Professor

Researcher basic information

■ Degree
  • 博士（農学）, 北海道大学
■ URL
researchmap URLホームページURL■ Various IDs
J-Global ID■ Research Keywords and Fields
Research Keyword
  • 家畜生産システム
  • 飼料
  • 乳牛
  • 放牧
  • 畜産学
  • 農林水産物
Research Field
  • Life Science, Animal production science
■ Educational Organization

Career

■ Career
Career
  • Oct. 2021 - Present
    Hokkaido University, Graduate School of Agriculture Research Faculty of Agriculture, 准教授, Japan
  • Apr. 2021 - Sep. 2021
    Hokkaido University, Field Science Center for Northern Biosphere, 准教授, Japan
  • Feb. 2015 - Mar. 2021
    Hokkaido University, Field Science Center for Northern Biosphere, 助教
  • Sep. 2014 - Jan. 2015
    Hokkaido University, Field Science Center for Northern Biosphere, 学術研究員
  • Sep. 2011 - Aug. 2014
    Hokkaido University, Graduate School of Agriculture Research Faculty of Agriculture, 特任助教
  • Jul. 2011 - Aug. 2011
    Hokkaido University, Graduate School of Agriculture Research Faculty of Agriculture, 博士研究員
  • Apr. 2009 - Jun. 2011
    Hokkaido University, Creative Research Institution, 特任助教
  • Apr. 2007 - Mar. 2009
    Hokkaido University, Creative Research Initiative 'Sousei', 博士研究員
  • Apr. 2006 - Mar. 2007
    農研機構 畜産草地研究所（つくば）, 研究補助員
  • Apr. 2005 - Mar. 2006
    National Agriculture and Food Research Organization, 特殊研究補助員
Educational Background
  • Apr. 2002 - Sep. 2005, Hokkaido University, 大学院農学研究科, 生物資源生産学専攻 博士後期課程
  • Apr. 2001 - Mar. 2002, Hokkaido University, 大学院農学研究科, 生物資源生産学専攻 博士前期課程
  • Apr. 1996 - Mar. 2000, Hokkaido University, Faculty of Agriculture, Department of Animal Science
  • Apr. 1994 - Mar. 1996, 私立 京都大谷高等学校
Committee Memberships
  • Apr. 2023 - Present
    生乳取扱者技術認定講習会運営委員会, 委員長
  • Apr. 2023 - Present
    日本酪農科学会, 評議員
  • Apr. 2023 - Mar. 2025
    北海道畜産草地学会, 庶務幹事
  • Apr. 2016 - Mar. 2021
    北海道畜産草地学会, 編集委員
  • Apr. 2016 - Mar. 2020
    北海道畜産草地学会, 監事, Society
  • Apr. 2012 - Mar. 2014
    北海道畜産草地学会, 会計幹事, Society
  • Apr. 2010 - Dec. 2012
    北海道家畜管理研究会, 会計幹事, Society

Research activity information

■ Papers
  • Effect of introducing somatic mitochondria into an early embryo on zygotic gene activation
    Yoshihiro Hayashi; Hanako Bai; Masashi Takahashi; Tomohiro Mitani; Manabu Kawahara
    Biology of Reproduction, Oxford University Press (OUP), 15 Jan. 2025, [Peer-reviewed]
    Scientific journal, Abstract

    Unlike differentiated somatic cells, which possess elongated mitochondria, undifferentiated cells, such as those of preimplantation embryos, possess round, immature mitochondria. Mitochondrial morphology changes dynamically during cell differentiation in a process called mitochondrial maturation. The significance of the alignment between cell differentiation and mitochondrial maturity in preimplantation development remains unclear. In this study, we analyzed mouse embryos into which liver-derived somatic mitochondria were introduced (SM-embryos). Most SM-embryos were arrested at the two-cell stage. Some of the introduced somatic mitochondria became round, while others remained elongated and large. RNA-sequencing revealed a disruption of both minor and major zygotic gene activation (ZGA) in SM-embryos. Minor ZGA did not terminate before major ZGA, and the onset of major ZGA was inhibited, as shown by histone modification analyses of histone H3 lysine 4 trimethylation and histone H3 lysine 27 acetylation. Further analysis of metabolites involved in histone modification regulation in SM-embryos showed a significantly lower NAD+/NADH ratio in SM-embryos than in control embryos. Additionally, the mitochondrial membrane potential, an indicator of mitochondrial function, was lower in SM-embryos than in control embryos. Our results demonstrated that introducing somatic mitochondria into an embryo induces mitochondrial dysfunction, thereby disrupting metabolite production, leading to a disruption in ZGA and inducing developmental arrest. Our findings reveal that the alignment between cell differentiation and mitochondrial maturity is essential for early embryonic development.
  • Effect of grain vinegar feeding on milk production and fatty acid profile of Holstein cows
    Seongjin Oh; Tomohiro Mitani; Masahito Kawai; Koichiro Ueda
    Journal of Animal Science and Technology, 66, 6, 1162, 1169, Korean Society of Animal Science and Technology, 30 Nov. 2024, [Peer-reviewed]
    Scientific journal
  • Application of glucose to prepare Aspergillus oryzae koji as an adjunct to prevent rancidity in cheese products.
    Napaporn Chintagavongse; Tomohiro Mitani; Koichi Tamano; Toru Hayakawa; Jun-Ichi Wakamatsu; Haruto Kumura
    Bioscience, biotechnology, and biochemistry, 28 Nov. 2024, [Peer-reviewed], [International Magazine]
    English, Scientific journal, Koji made using Aspergillus oryzae shows potential for application as a cheese adjunct; however, flavor defects resulting from volatile free fatty acid (FFA) accumulation should be avoided. Hence, a modified glucose-containing whey solid medium was used to culture A. oryzae AHU 7139, and the triacylglycerol (TG) lipase activity and lipase gene (tglA and mdlB) expression were compared with those of a culture using a conventional whey solid medium. The results showed that TG lipase activity and the expression of both lipase genes were reduced in the modified medium. Moreover, the expression level of farA, a positive transcription factor of the lipase genes, was also reduced. The cheese adjunct prepared by culturing the AHU 7139 strain in the modified medium lowered the FFA content in the cheese products, resulting in comparable FFA levels with those in the adjunct-free cheese. Thus, adding glucose is recommended to prepare the koji adjunct for cheesemaking.
  • Relationship between feeding management of dairy farm, and chemical compositions, aroma compounds, and sensory property of milk
    三谷朋弘; 朝隈貞樹; 篠田優香; 上田靖子
    ミルクサイエンス, 72, 2, 45, 53, Aug. 2023, [Lead author]
  • APPLICABILITY OF VITAL DATA COLLECTED FROM A NON-CONTACT SENSOR FOR ESTIMATING AN INDIVIDUAL COW’S METHANE EMISSION WITH A LASER METHANE DETECTOR
    Samuel Kinari SAGA; Shiho ISHIKAWA; Tomohiro MITANI; Shigeru MORITA; Ryoichi HARA; Takayuki TANAKA; Michio KOMIYA; Kazuei ISHII; Satoru OCHIAI; Geun-Yong HAM
    Journal of JSCE, 11, 2, 1, 9, Japan Society of Civil Engineers, 2023, [Peer-reviewed]
    English, Scientific journal
  • A study of lipolysis induced by adjuncts from edible Aspergillus sp. solid culture products on ripened semi-hard cheese.
    Napaporn Chintagavongse; Hayate Takiguchi; Chi Ming-Hsuan; Koichi Tamano; Toru Hayakawa; Jun-Ichi Wakamatsu; Tomohiro Mitani; Haruto Kumura
    Journal of the science of food and agriculture, 23 Jan. 2022, [Peer-reviewed], [International Magazine]
    English, Scientific journal, BACKGROUND: Aspergillus sp. has been used in traditional Japanese fermented foods. Protease-containing culture products of A. oryzae have been applied as the adjunct enzyme source to enrich the flavor in ripened cheese. Although proteolysis was stimulated, the increase of free fatty acids (FFA) was recognized in some products. Since an excess amount of FFA accumulation can cause rancidity in cheese products, the assessment of lipase activity was considered to be essential for the cheese adjunct preparation. RESULTS: Although an equal lipase activity from the adjunct materials of A. kawachii NBRC 4308, A. luchuensis RIB 2604 and A. oryzae AHU 7139 was applied to semi-hard cheese, the FFA level was significantly higher in A. oryzae cheese than in the others. Furthermore, the profiles of volatile components were different in experimental cheeses. An in vitro study with experimental curds demonstrated that the high FFA might not depend on the lipase retainability on curds. On the contrary, the pronounced activation of the lipases occurred in A. oryzae after incubation with the curds. Moreover, incubation of the insoluble lipase that had been attached to the cells with skim milk curd extracts allowed the release of lipases from the cells into the medium with remarkable activation. CONCLUSION: A. oryzae AHU 7139 possessed a complex lipolytic system comprising extracellular and cell-binding lipases that were attributed to the increase in FFA in A. oryzae cheese. © 2022 Society of Chemical Industry.
  • Application of MinION Amplicon Sequencing to Buccal Swab Samples for Improving Resolution and Throughput of Rumen Microbiota Analysis.
    Hiroto Miura; Masayuki Takeda; Megumi Yamaguchi; Yoshihisa Ohtani; Go Endo; Yasuhisa Masuda; Kaede Ito; Yoshio Nagura; Kunihiro Iwashita; Tomohiro Mitani; Yutaka Suzuki; Yasuo Kobayashi; Satoshi Koike
    Frontiers in microbiology, 13, 783058, 783058, 2022, [Peer-reviewed], [International Magazine]
    English, Scientific journal, The Illumina MiSeq platform has been widely used as a standard method for studying the rumen microbiota. However, the low resolution of taxonomic identification is the only disadvantage of MiSeq amplicon sequencing, as it targets a part of the 16S rRNA gene. In the present study, we performed three experiments to establish a high-resolution and high-throughput rumen microbial profiling approach using a combination of MinION platform and buccal swab sample, which is a proxy for rumen contents. In experiment 1, rumen contents and buccal swab samples were collected simultaneously from cannulated cattle (n = 6) and used for microbiota analysis using three different analytical workflows: amplicon sequencing of the V3-V4 region of the 16S rRNA gene using MiSeq and amplicon sequencing of near full-length 16S rRNA gene using MinION or PacBio Sequel II. All reads derived from the MinION and PacBio platforms were classified at the species-level. In experiment 2, rumen fluid samples were collected from beef cattle (n = 28) and used for 16S rRNA gene amplicon sequencing using the MinION platform to evaluate this sequencing platform for rumen microbiota analysis. We confirmed that the MinION platform allowed species-level taxa assignment for the predominant bacterial groups, which were previously identified at the family- and genus-level using the MiSeq platform. In experiment 3, buccal swab samples were collected from beef cattle (n = 30) and used for 16S rRNA gene amplicon sequencing using the MinION platform to validate the applicability of a combination of the MinION platform and buccal swab samples for rumen microbiota analysis. The distribution of predominant bacterial taxa in the buccal swab samples was similar to that in the rumen samples observed in experiment 2. Based on these results, we concluded that the combination of the MinION platform and buccal swab samples may be potentially applied for rumen microbial analysis in large-scale studies.
  • Cow Milk Progesterone Concentration Assessment during Milking Using Near-infrared Spectroscopy
    Patricia IWEKA; Shuso KAWAMURA; Tomohiro MITANI; Takashi KAWAGUCHI; Shigenobu KOSEKI
    Engineering in Agriculture, Environment and Food, 14, 1, 30, 36, Asian Agricultural and Biological Engineering Association, 2021, [Peer-reviewed]
    Scientific journal
  • Effects of grazing adaptation on herbage intake, milk production, and body weight change in lactating dairy cows after turning out to pasture in early spring
    Mitani, T; Nakajima, S; Oh, S; Kawai, M; Ueda, K
    Animal Science Journal, 92, 1, e13639, 2021, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
    English, Scientific journal, The present study investigated the effects of adaptation to grazing in early spring on the herbage intake, milk production, and body weight changes in lactating dairy cows. The experiment included 12 Holstein lactating cows during early spring. Six cows were allowed to gradually adapt to grazing (ADP) over 10 days. They were allowed to graze on a pasture for 4 h during the first week and for 19 h during the remaining 3 days (adaptation period). During the 10-day period, the remaining six cows were housed in a barn (CON). Cows in both groups received adequate silage, hay, and concentrate during the adaptation period. Subsequently, cows in both groups were allowed to graze together for 19 h for 4 weeks (experimental period). No cow received supplements during the experimental period. In the first week of the experimental period, the ADP cows showed a higher herbage intake than the CON cows. During the subsequent weeks, there were no differences in herbage intake between the two groups. At the start of the experimental period, milk production and body weight of the ADP cows were higher than those of the CON cows, and this difference lasted until the end of the experiment.
  • Regional differences in the fatty acid composition, and vitamin and carotenoid concentrations in farm bulk milk in Hokkaido, Japan.
    Mitani, T; Kobayashi, K; Ueda, K; Kondo, S
    Animal Science Journal, 92, 1, e13570, 2021, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
    English, Scientific journal, In this study, we investigated the regional differences in the composition of farm bulk milk produced at three different dairy areas in Hokkaido, Japan. A field survey was conducted at Central, Tokachi, and North areas of Hokkaido, three or four times a year. At each farm, an interview questionnaire for farm basal data was conducted, and 500 ml of bulk tank milk sample was obtained. Fatty acid composition, and vitamin and carotenoid concentrations in the milk samples were determined. In Central and Tokachi areas, corn silage was used as the main forage. In North area, fresh herbage was the dominant feed in the summer season, and grass hay was the main feed in the winter season. Discriminant analysis revealed that the composition of milk samples differed among the areas and seasons. Milk from Central and Tokachi areas contained a higher ratio of linoleic acid compared with that from North area, but there were only slight differences in the composition of milk between Central and Tokachi areas. The concentrations of carotenoids and α-tocopherol were higher in samples from North area and the ratios of trans-vaccenic acid and conjugated linoleic acid were higher in the summer season than in the indoor season.
  • Evaluation of taste sensing system for discriminating milk produced by different feeding management of cows and heat treatments.
    Mitani, T; Asakuma, S; Ueda, Y; Uchida, K; Kawamura, S; Ueda, K; Akiyama, F
    Milk Science, 70, 1, 14, 21, 2021, [Peer-reviewed], [Lead author, Corresponding author]
    English
  • Effects of grazing adaptation on intake, ruminal fermentation, blood metabolites, and body weight change in dairy cows after turning out to pasture in early spring.
    Mitani, T; Kubota, T; Mizuguchi, H; Kushibiki, S; Ueda, K
    Animal Science Journal, 91, 1, e13481, 2020, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
    English, Scientific journal, This study investigated the effect of adaptation to grazing in early spring on herbage intake, ruminal fermentation parameters, blood metabolite concentrations, and body weight change in dairy cows. The experiment was conducted on eight rumen-cannulated non-lactating cows in the early spring period. Four cows were adapted to grazing by stocking for 4 hr for 1 week (ADP group). The other cows were kept in a barn during the period (CON group). Then, both groups of cows were stocked together throughout a day on a 1 ha pasture for 3 weeks (experimental period). In the first week of the experimental period, compared to the CON group, the ADP group had a higher herbage intake, ruminal NH3 -N and total VFA concentration, and blood urea concentration, but the NEFA concentration was lower in the ADP group (p < .01). During the subsequent weeks, there were little differences in ruminal fermentation parameters and blood metabolites. Cows in the ADP group maintained their body weight, but cows in the CON group lost 60 kg of body weight in the first week of the experimental period.
  • Effect of summer heat stress on gene expression in bovine uterine endometrial tissues
    Bai, H; Ukita, H; Kawahara, M; Mitani, T; Furukawa, E; Yanagawa, Y; Yabuuchi, N; Kim, H; Takahashi
    Animal Science Journal, 91, 1, e13454, Wiley, 2020, [Peer-reviewed]
    English, Scientific journal
  • Effects of ear corn silage supplementation on milk production and milk fatty acid profiles in grazing dairy farms.
    Mitani, T; Asakuma, S; Shinoda, Y; Ueda, Y; Aoki, Y; Oshita
    Animal Science Journal, 91, e13474, 2020, [Peer-reviewed], [Lead author, Corresponding author]
    English, Scientific journal
  • Non-destructive determination of bovine milk progesterone concentration during milking using near-infrared spectroscopy.
    Iweka, P; Kawamura, S; Mitani, T; Koseki, S
    Agricultural Engineering International: CIGR Journal, 22, 171, 178, 2020, [Peer-reviewed]
    English, Scientific journal
  • The changes of grazing behavior and locomotion activity per day for stocked Thoroughbred foals with growth.
    Tanabe, T; Mitani, T; Ueda, K; Matsui, A; Kawai, M
    Animal Behaviour and Management, 56, 2, 55, 62, 2020, [Peer-reviewed]
    English
  • Online milk quality assessment during milking using near-infrared spectroscopic sensing system
    Patricia Iweka; Shuso Kawamura; Tomohiro Mitani; Takashi Kawaguchi; Shigenobu Koseki
    Environmental Control in Biology, 58, 1, 1, 6, 2020, [Peer-reviewed]
    Scientific journal
  • The relationship between the concentrate of free fatty acid in bulk tank milk and milking traits in a commercial dairy farm that uses an automatic milking system
    MORITA Shigeru; Megumi Hiramatsu; Fuko Oka; Tomohiro Mitani; Yasutaka Kumano; Eijiro Koita; Naoko Iida
    Animal Behaviour and Management, 55, 1, 1, 7, Mar. 2019, [Peer-reviewed]
    Japanese
  • Cow milk progesterone concentration determination during milking using near-infrared spectroscopy.
    Iweka, P; Kawamura, S; Mitani, T; Kawaguchi, T; Koseki, S
    Proceedings of International Joint Conference on JSAM, SASJ, and 13th CIGR VI Technical Symposium joining FWFNWG and FSWG Workshops, 2019
    English, International conference proceedings
  • Quality and flavor of milk related with feeding management for dairy cows
    三谷朋弘
    乳業技術, 69, 1, 11, 2019, [Invited]
    Japanese
  • Wall thickness control in biotubes prepared using type-C mold
    Takeshi Terazawa; Takanori Nishimura; Tomohiro Mitani; Osamu Ichii; Teppei Ikeda; Keigo Kosenda; Eisuke Tatsumi; Yasuhide Nakayama
    Journal of Artificial Organs, 21, 3, 1, 5, Springer Tokyo, 30 Mar. 2018, [Peer-reviewed]
    English, Scientific journal
  • Timing strategy for concentrate supplementation in grazing dairy cows
    Ueda, K; Mitani, T
    Proceedings of the 7th Japan-China-Korea Grassland Conference, Japan, 2018, [Invited]
    English, International conference proceedings
  • Non-destructive online real-time milk quality determination in a milking robot using near-infrared spectroscopic sensing system
    Patricia Iweka; Shuso Kawamura; Tomohiro Mitani; Shigenobu Koseki
    Arid Zone Journal of Engineering, Technology and Environment, 18, 121, 128, 2018, [Peer-reviewed]
    English, Scientific journal
  • Effect of feeding condensed sweet-potato distillers solubles in fermented TMR on milk production and milk flavor
    鈴木知之; 三谷朋弘; 神谷裕子; 田中正仁; 服部育男; 野中最子; 佐藤健次
    日本畜産学会報, 89, 1, 37, 45, 2018, [Peer-reviewed]
    Japanese
  • 高乳量水準の酪農場における最短搾乳間隔時間の延長が自動搾乳システムでの牛乳生産量および搾乳特性に及ぼす影響
    森田 茂; 高橋圭二; 三谷朋弘; 熊野康隆; 小板英次郎; 飯田直子
    北海道畜産草地学会報, 6, 35, 39, 2018, [Peer-reviewed]
    Japanese
  • Effect of grazing and processing treatments on sensory characteristics of cow milk
    Asakuma Sadaki; Ueda Yasuko; Akiyama Fumiaki; Uchida Kenji; Katano Naoya; Kawamura Shuso; Mitani Tomohiro
    Milk Science, 67, 1, 22, 29, Japanese Dairy Science Association, 2018, [Peer-reviewed]
    Japanese, Scientific journal,

    Over 50% of milk productions in Japan have been performed in Hokkaido region, where there are larger grassland area than in other region of Japan. The purpose of the study is to evaluate the sensory characteristics of twelve milks, produced by four farmers, three of whom performed the grazing fed, while one performed non-grazing fed. These milks were processed by each of three kinds of the treatments; ultra-high temperature treatment (UHT: 120℃, 2 s) with homogenization (U+H), high temperature short time treatment (HTST: 72℃, 15 s) with homogenization (H+H), and HTST treatment without homogenization (H+N). Sensory evaluation was conducted by 60 consumer panelists. The panelists were divided into four groups by 6×6 Latin square design. The evaluation was conducted four times per a day, or three times per a day in these experiments. Twelve milk samples (four farmer's bulk milk treated by either of the above processing method) were scored for seven items, i. e. the taste of koku, sweetness, aroma strength with −5 (weak) to 5 (strong), aftertaste feeling, as well as aroma preference with favorability at −5 (unfavorable) to 5 (favorable) at each ±5 scoring stages, compared with those of a commercial UHT milk as base (zero). The evaluation scores of 12 milk samples, analyzed by the principle component analysis, could be differentiated among three processing treatments and also between the milk from grazing cows or non-grazing cows. In preference map, it was suggested that the homogenized milk of grazing cows was rather favorable than that of non-homogenized milk.

  • Effects of the presence of grazing-experienced heifers on the development of foraging behavior at the feeding station scale for first-grazing season calves
    Yuko Shingu; Koichiro Ueda; Shingo Tada; Tomohiro Mitani; Seiji Kondo
    ANIMAL SCIENCE JOURNAL, 88, 8, 1120, 1127, Aug. 2017, [Peer-reviewed]
    English, Scientific journal
  • Skill reading of human social cues by horses (Equus caballus) reared under year-round grazing conditions
    Ryoko Koizumi; Tomohiro Mitani; Koichiro Ueda; Seiji Kondo
    Animal Behaviour and Management, 53, 2, 69, 78, 日本家畜管理学会, 2017, [Peer-reviewed]
    English, Scientific journal
  • Effect of timing and type of supplementary grain on herbage intake, nitrogen utilization and milk production in dairy cows grazed on perennial ryegrass pasture from evening to morning
    Koichiro Ueda; Tomohiro Mitani; Seiji Kondo
    ANIMAL SCIENCE JOURNAL, 88, 1, 107, 118, Jan. 2017, [Peer-reviewed]
    English, Scientific journal
  • Effect of increased concentrate allotment before evening grazing on herbage intake, nitrogen utilization and rumen fermentation in dairy cows grazed on perennial ryegrass pasture
    Koichiro Ueda; Tomohiro Mitani; Seiji Kondo
    ANIMAL SCIENCE JOURNAL, 87, 10, 1233, 1243, Oct. 2016, [Peer-reviewed]
    English, Scientific journal
  • Relationship of rumen fill and fermentation to diurnal and seasonal variation of herbage intake in dairy cows grazed on perennial ryegrass pasture
    Koichiro Ueda; Tomohiro Mitani; Seiji Kondo
    ANIMAL SCIENCE JOURNAL, 87, 9, 1148, 1156, Sep. 2016, [Peer-reviewed]
    English, Scientific journal
  • Effect of water-soluble carbohydrate content in orchardgrass pasture on grazing time and rumen fermentation in dairy cows
    Koichiro Ueda; Tomohiro Mitani; Seiji Kondo
    ANIMAL SCIENCE JOURNAL, 87, 9, 1122, 1129, Sep. 2016, [Peer-reviewed]
    English, Scientific journal
  • Herbage intake and ruminal digestion of dairy cows grazed on perennial ryegrass pasture either in the morning or evening
    Koichiro Ueda; Tomohiro Mitani; Seiji Kondo
    ANIMAL SCIENCE JOURNAL, 87, 8, 997, 1004, Aug. 2016, [Peer-reviewed]
    English, Scientific journal
  • Discrimination of "grazing milk" using milk fatty acid profile in the grassland dairy area in Hokkaido
    Tomohiro Mitani; Kuniyuki Kobayashi; Koichiro Ueda; Seiji Kondo
    ANIMAL SCIENCE JOURNAL, 87, 2, 233, 241, Feb. 2016, [Peer-reviewed], [Lead author, Corresponding author]
    English, Scientific journal
  • 北海道和種繁殖母馬のヒト許容距離と子馬のヒト許容距離の関係(日本家畜管理学会・応用動物行動学会2016年度春季合同研究発表会)
    納多 春佳; 河合 正人; 三谷 朋弘; 上田 宏一郎; 近藤 誠司
    日本家畜管理学会誌・応用動物行動学会誌, 52, 1, 54, 54, 日本家畜管理学会, 2016
    Japanese
  • Effect of Cow individuality on accuracy of calibration models using near-infrared spectroscopy for determining milk constituents during milking
    Iweka, P; Kawamura, S; Morita, A; Mitani, T; Okatani, T; Koseki, S
    Proceedings of the 8th International Symposium on Machinery and Mechatronics for Agriculture and Biosystems Engineering (ISMAB), 2016
    English, International conference proceedings
  • Regional difference of feeding management and milk composition in Hokkaido, Japan
    Tomohiro Mitani; Kuniyuki Kobayashi; Tetsushiro Endo; Koichiro Ueda; Seiji Kondo
    Proceedings of the 6th Korea-China-Japan Grassland Conference, 86, 89, 2016, [Invited]
    English, International conference proceedings
  • Large-Scale Glycomics of Livestock: Discovery of Highly Sensitive Serum Biomarkers Indicating an Environmental Stress Affecting Immune Responses and Productivity of Holstein Dairy Cows
    Ibrahim F. Rehan; Koichiro Ueda; Tomohiro Mitani; Maho Amano; Hiroshi Hinou; Tetsu Ohashi; Seiji Kondo; Shin-Ichiro Nishimura
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 63, 48, 10578, 10590, Dec. 2015, [Peer-reviewed]
    English, Scientific journal
  • Structure of subgroup in mares and foals in a herd of reproductive horse and formation change of subgroup in weaned foals
    Sato, F; Tada, S; Mitani, T; Ueda, K; Kondo, S
    Proceedings of the 49th Congress of the International Society for Applied Ethology, 2015
    English, International conference proceedings
  • Relationship between flight distance of mare and foal to human in Hokkaido native horse.
    Noda, H; Tada, S; Mitani, T; Ueda, K; Kondo, S
    Proceedings of the 49th Congress of the International Society for Applied Ethology, 2015
    English, International conference proceedings
  • Effect of substitution of highly-digestible fiber of beet pulp for corn grain starch in concentrate on energy balance and recovery of ovarian function in early lactating dairy cows
    Bo M; Masaki, T; Nagano, M; Yanagawa, Y; Mitani, T; Ueda, K; Kondo, S
    Hokkaido Journal of Livestock and Grassland Science, 3, 45, 53, 2015, [Peer-reviewed]
    English, Scientific journal
  • Long-Term Oral Feeding of Lutein-Fortified Milk Increases Voluntary Running Distance in Rats
    Megumi Matsumoto; Masahito Hagio; Ryo Inoue; Tomohiro Mitani; Masako Yajima; Hiroshi Hara; Takaji Yajima
    PLOS ONE, 9, 4, e93529, Apr. 2014, [Peer-reviewed]
    English, Scientific journal
  • Effects of a proportion of herbage intake and crude protein content of herbage on nitrogen and energy utilization in lactating dairy cows
    Tomohiro Mitani; Koichiro Ueda; Tetsushiro Endo; Makoto Takahashi; Hiroki Nakatsuji; Seiji Kondo
    Hokkaido Journal of Livestock and Grassland Science, 2, 51, 62, 北海道畜産草地学会, 2014, [Peer-reviewed], [Lead author, Corresponding author]
    English, Scientific journal
  • Diversity of dairy farming and milk composition in Japan and Hokkaido
    Mitani, T
    IDF World Dairy Summit 2013, 2013, [Invited]
    English, International conference proceedings
  • Relationship between sensory property and chemical composition of raw milk in different feeding management of Hokkaido in Japan
    Mitani, T; Katoh, H; Mimuro, A; Ohyama, K; Ueda, K; Kondo, S
    IDF World Dairy Summit 2013, 2013
    English, International conference proceedings
  • Regional difference of fatty acid, vitamin and β-carotene concentration in milk of Hokkaido in Japan
    Mitani, T; Kobayashi, K; Ueda. K; Komatsu, Y; Kondo. S
    IDF World Dairy Summit 2012, 2012
    English, International conference proceedings
  • 草からの牛乳生産の研究
    三谷朋弘
    北海道畜産学会報, 54, 46, 1, 5, 2012
    Japanese
  • Effect of feeding non-fibrous carbohydrate before grazing on intake and nitrogen utilization in dairy cows throughout the grazing season
    Tomohiro Mitani; Koichiro Ueda; Tetsushiro Endo; Makoto Takahashi; Hiroki Nakatsuji; Seiji Kondo
    ANIMAL SCIENCE JOURNAL, 83, 2, 121, 127, 2012, [Peer-reviewed]
    English, Scientific journal
  • Change in milk compositions from cows during transition period from barn feeding to grazing
    三谷朋弘; 佐藤悠二; 上田宏一郎; 高橋誠; 中辻浩喜; 近藤誠司
    北海道畜産学会報, 53, 29, 34, 2011, [Peer-reviewed]
    Japanese, Scientific journal
  • Effects of the Addition of Urea or Soy Sauce Cake to Potato Pulp on the Fermentative Quality of Silage, Nutritive Value and Nitogen Balance in Sheep.
    大下友子; 三谷朋弘; 宮地慎; 青木康浩; 秋山典昭
    日本草地学会誌, 55, 4, 201, 207, 2010, [Peer-reviewed]
    Japanese
  • 乳牛の分娩後黄体機能の回復に対するセレン酵母給与の影響
    鎌田 八郎; 上田 靖子; 三谷 朋弘; 宮地 慎; 安井 喬; 中田 健; 松井 義貴; 村井 勝
    日本畜産学会大会講演要旨集, 111回, 49, 49, (公社)日本畜産学会, Sep. 2009
    Japanese
  • Effect of Set Stocking and Rotational Grazing by Lactating Dairy Cows on Sward Structure, Herbage Production and Utilization
    遠藤哲代; 三谷朋弘; 高橋誠; 上田宏一郎; 中辻浩喜; 近藤誠司
    日本草地学会誌, 55, 1, 9, 14, 2009, [Peer-reviewed]
    Japanese
  • 農村におけるネットワークの構造と特質
    小林国之; 三谷朋弘
    日本農業経済学会論文集，2008年度農業経済研究別冊, 137, 144, 2008, [Peer-reviewed]
    Japanese, Scientific journal
  • Differences of lying-standing action and locomotion posture between cows kept on pasture and in cowshed
    Kondo, S; Takahashi, M; Mitani, T; Kashiwabara, H; Ueda, K; Nakatsuji, H
    Proceedings of the 41st International Congress of the International Society for Applied Ethology, 2007
    English, International conference proceedings
  • 反芻胃でのタンパク質と炭水化物の分解同期化を考慮した放牧乳牛に対する併給飼料の給与法
    三谷朋弘; 遠藤哲代; 高橋 誠; 上田宏一郎; 中辻浩喜; 近藤誠司
    栄養生理研究会報, 52, 1, 1, 9, 2007, [Peer-reviewed]
    Japanese
  • Chemical Composition, Characteristics of Silage Fermentation and Nutritive Value of Potato Pulp Silages with or without Lactic Acid Bacteria Innoculants.
    大下友子; 三谷朋弘; 宮地慎; 青木康浩; 秋山典昭
    日本草地学会誌, 53, 3, 201, 207, 2007, [Peer-reviewed]
    Japanese
  • Effects of pre-feeding of a corn silage-based supplement on the feed intake, milk production and nitrogen utilization of grazing dairy cows
    Tomohiro Mitani; Makoto Takahashi; Koichiro Ueda; Hiroki Nakatsuji; Seiji Kondo
    Animal Science Journal, 76, 5, 453, 460, Oct. 2005, [Peer-reviewed]
    English, Scientific journal
  • Effects of supplementary corn silage on the feed intake and milk production of time-restricted grazing dairy cows
    Tomohiro Mitani; Makoto Takahashi; Koichiro Ueda; Hiroki Nakatsuji; Seiji Kondo; Masahiko Okubo
    Animal Science Journal, 76, 4, 331, 337, Aug. 2005, [Peer-reviewed]
    English, Scientific journal
  • 「泌乳牛の放牧飼養時における反芻胃内でのタンパク質と炭水化物分解の同期化に関する研究
    三谷 朋弘
    2005
    Japanese, Doctoral thesis
  • The effect of stocking rate and initial grass height on herbage production and utilization, and milk production per unit area under set stocking by lactating dairy cows
    H. Nakatsuji; Endo, T; Bawm, S; Mitani, T; Ueda K; Kondo, S
    Proceedings of a satellite workshop of the XXth International Grassland Congress, 240, 2005
    English, International conference proceedings
  • Effect of pre-grazing feeding of a non-fibrous carbohydrate on feed intake, milk production and nitrogen utilization
    Tomohiro Mitani; Junki Honma; Tetsushiro Endo; Koichiro Ueda; Hiroki Nakatsuji; Seiji Kondo
    Tomohiro Mitani, Junki Honma, Tetsushiro Endo, Koichiro Ueda, Hiroki Nakatsuji, Seiji Kondo, 31, 37, 2005
    English, International conference proceedings
  • 泌乳牛のコーンサイレージ主体飼養下におけるルーメン内分解性蛋白質含量の異なる濃厚飼料の給与が乳生産、窒素およびエネルギー出納に及ぼす影響
    中辻浩喜; 竹井 悠; 三谷朋弘; 近藤誠司; 大久保正彦
    北海道大学大学院農学研究科邦文紀要, 24, 3-4, 381, 388, 2005, [Peer-reviewed]
    Japanese
  • Effect of timing to feed corn silage-based supplement for grazing dairy cows on intake, milk production and nitrogen utilization.
    Mitani, T; Takahashi, M; Ueda, K; Nakatsuji, H; Kondo, S
    55th Annual Meeting of the European Association for Animal Production, 2004
    English, International conference proceedings
  • Herbage production and utilization, and milk production per unit area under set stocking and rotational grazing by lactating dairy cows
    Nakatsuji, H; Endo, T; Kurata, M; Mitani, T; Takahashi, M; Ueda, K; Kondo, S
    Proceedings of the 11th Animal Science Congress, 517, 519, 2004
    English, International conference proceedings
  • Effect of different concentrate supplementation on milk production, rumen fermentation, nitrogen and energy balance in grazing dairy cows
    Nakatsuji, H; Takei, Y; Mitani, T; Kondo, S; Okubo, M
    26th IDF World Dairy Congress, 2002
    English, International conference proceedings
  • Effect of corn silage supplementation on intake, milk production, energy and nitrogen balance in grazing dairy cows
    Mitani, T; Nakatsuji, H; Kondo S; Okubo, M
    26th IDF World Dairy Congress, 2002
    English, International conference proceedings
■ Other Activities and Achievements
■ Books and other publications
  • 乳肉卵の機能と利用 新版
    三谷朋弘, 2章 乳の科学, Section 1 乳脂肪の合成と性質，分析法
    アイ・ケイ・コーポレーション, 2018, [Contributor]
  • 日本飼養標準 乳牛（2017年版）
    三谷朋弘, 5.3 乳成分に及ぼす給与飼料の影響
    国立研究法人 農業・食品産業技術総合研究機構 編, 2017, [Contributor]
  • Milk book ～とっておきレシピ＆牛乳のお話
    三谷朋弘
    北海道新聞社, 2011, [Contributor]
  • 酪農大辞典
    三谷朋弘, 生理・飼育技術・環境管理
    農文協, 2011, [Contributor]
  • 最新農業技術 畜産 vol.3
    三谷朋弘, 牛乳の脂肪酸組成-舎飼いと放牧での比較
    農文協, 2010, [Contributor]
■ Lectures, oral presentations, etc.
  • 「生乳の風味異常における発生メカニズムと防止対策」
    三谷 朋弘
    令和3年度 北海道農業団体生乳検査技術研究会 研修会, 28 Jun. 2021
    [Invited]
  • 「より良い風味の牛乳の生産に向けて -酪農現場における自発性酸化臭発生メカニズムの解明-」
    三谷 朋弘; 森田 茂
    生乳の風味に関する研究事業報告説明会, 08 Jun. 2021
    08 Jun. 2021 - 08 Jun. 2021, [Invited]
  • 「酪農生産現場における自発性酸化臭発生メカニズムとその要因について」
    三谷 朋弘
    令和２年度生乳検査技術者連絡会, 24 Feb. 2021
    [Invited]
  • 「生乳の異常風味改善」
    三谷 朋弘
    令和元年度経営体育成対策事業酪農ゼミナール, 18 Feb. 2020
    [Invited]
  • 「乳中の脂肪酸組成 －その生成メカニズムと飼料との関連－」
    三谷 朋弘
    令和元年度生乳検査技術者連絡会, 29 Jan. 2020
    [Invited]
  • 北海道における乳牛飼養最前線
    三谷朋弘
    北海道畜産草地学会第8回大会シンポジウム「温故知新～北海道の酪農畜産の持続的発展を目指して～」, 25 Aug. 2019
    [Invited]
  • 「生乳の風味変化について －特に、自発性酸化臭について－」
    三谷朋弘
    令和元年度第1回生乳の安全・安心の確保のための指定団体担当者会議, 12 Jul. 2019
    [Invited]
  • 生乳の風味変化に関わる成分分析 －特に、乳脂肪に関わる成分について－
    三谷朋弘
    平成30年度生乳検査技術者連絡会, Feb. 2019
    [Invited]
  • 生乳の風味変化について －特に、自発性酸化臭について－
    三谷朋弘
    令和元年度 安全・安心の確保のための指定団体担当者会議, 2019
    [Invited]
  • 生乳の風味異常について
    三谷朋弘; 森田 茂
    Jミルク 生乳の異常風味研修会, 2019
    [Invited]
  • 生乳の風味変化について －その発生メカニズムと要因について－
    三谷朋弘
    平成30年度 全国生乳検査技術研修会, Oct. 2018
    [Invited]
  • 国産飼料100%による牛乳生産に向けて -放牧牛に対するイアコーンサイレージの給与事例-
    三谷朋弘
    NON-GMO生乳生産者・よつ葉共同購入グループ 2017年度 産地交流会, Jan. 2018
    [Invited]
  • 牛乳から見える文化
    三谷朋弘
    2016 北海道放牧酪農ネットワーク交流会in足寄, 30 Aug. 2016
    [Invited]
  • Regional difference of feeding management and milk composition in Hokkaido, Japan
    Tomohiro MITANI
    The 6th Korea-China-Japan Grassland Conference, Aug. 2016
    [Invited]
  • Diversity of dairy farming and milk composition in Japan and Hokkaido
    Tomohiro Mitani; Koji Ohyama; Seiji Kondo
    IDF World Dairy Summit 2013, Oct. 2013
    [Invited]
  • 草からの牛乳生産の研究
    三谷朋弘
    北海道畜産学会・北海道草地研究会・北海道家畜管理研究会合同シンポジウム, Dec. 2011, Keynote oral presentation
    [Invited]
  • Relationship between feeding system and milk characteristics in Hokkaido Region of Japan
    Tomohiro MITANI
    The 14th SNU-HU Joint Symposium, Nov. 2011
    [Invited]
  • 北海道における飼養方法の違いと牛乳の特徴について
    三谷朋弘
    平成23年度第1回地域交流牧場全国連絡会北海道ブロック, 14 Jul. 2011
    [Invited]
  • 高付加価値化牛乳の要件
    三谷朋弘
    「放牧牛乳のプレミアム化に向けた家畜健全性指標の確立と牛乳中機能性成分制御技術の開発」研究成果発表会, Mar. 2010
    [Invited]
  • 反芻胃でのタンパク質と炭水化物の分解同期化を考慮した放牧乳牛に対する併給飼料の給与法
    三谷朋弘; 遠藤哲代; 高橋 誠; 上田宏一郎; 中辻浩喜; 近藤誠司
    家畜栄養生理研究会春季集談会, Mar. 2008
    [Invited]
  • Effect of pre-grazing feeding of a non-fibrous carbohydrate on feed intake, milk production and nitrogen utilization
    T. Mitani; J. Honma; T. Endo; K. Ueda; H. Nakatsuji; S. Kondo
    The 13th Asian Diary Conference, Oct. 2005
    [Invited]
■ Syllabus
  • 家畜生態学特論, 2024年, 修士課程, 農学院
  • 大学院共通授業科目（教育プログラム）：食の安全・安心基盤学, 2024年, 修士課程, 大学院共通科目
  • 実践農学総論, 2024年, 修士課程, 農学院
  • ﾌｨーﾙﾄﾞ科学基礎論, 2024年, 修士課程, 環境科学院
  • 牧場実習, 2024年, 学士課程, 農学部
  • 畜産科学概論, 2024年, 学士課程, 農学部
  • 一般教育演習(ﾌﾚｯｼｭﾏﾝｾﾐﾅｰ), 2024年, 学士課程, 全学教育
  • 一般教育演習(ﾌﾚｯｼｭﾏﾝｾﾐﾅｰ), 2024年, 学士課程, 全学教育
  • 基礎家畜生産学Ⅲ, 2024年, 学士課程, 農学部
  • 家畜生産実習, 2024年, 学士課程, 農学部
  • 畜牧体系科学実験, 2024年, 学士課程, 農学部
■ Affiliated academic society
  • Dec. 2019
    日本酪農科学会
  • HOKKAIDO SOCIETY OF LIVESTOCK AND GRASSLAND SCIENCE
  • 動物の行動と管理学会
  • JAPANESE SOCIETY OF GRASSLAND SCIENCE
  • JAPANESE SOCIETY OF ANIMAL SCIENCE
■ Research Themes
  • 酪農家飼養環境とバルク乳成分調査事業
    Apr. 2021 - Mar. 2024
    三谷 朋弘, 森田 茂, 小林 国之, 朝隈 貞樹, 上田 靖子, 篠田 優香, 福澤 萌
    日本中央競馬会畜産振興事業, Principal investigator
  • 異なる飼養条件下で生産された牛乳の風味および品質特性
    ミルクサイエンス研究助成金
    Apr. 2020 - Mar. 2022
    三谷 朋弘; 朝隈 貞樹; 上田 靖子; 篠田 優香
    日本酪農科学会, 北海道大学, Principal investigator
  • 牧草サイレージの高品質化に向けたバイオガスプラント消化液施用量の評価
    令和2年度研究助成
    Apr. 2020 - Mar. 2021
    三谷 朋弘
    伊藤記念財団, 北海道大学, Principal investigator
  • 次世代閉鎖型搾乳牛舎とロボット、ICTによる省力化スマート酪農生産の実証 「搾乳作業の自動化の検証と個体別牛乳の品質分析装置の開発」
    国立研究開発法人農業・食品産業技術総合研究機構委託事業「スマート農業技術の開発・実証プロジェクト」及び「スマート農業加速化実証プロジェクト」
    Apr. 2019 - Mar. 2021
    三谷朋弘, 川村 周三
    国立研究開発法人農業・食品産業技術総合研究機構, Coinvestigator, Competitive research funding
  • 生乳の異常風味発生に対する対策事業
    日本中央競馬会畜産振興事業
    Apr. 2018 - Mar. 2021
    三谷朋弘, 福澤 萌, 森田 茂
    日本中央競馬会, Principal investigator, Competitive research funding
  • 道産トウモロコシの安定供給に基づく高付加価値畜産物生産技術の開発 「道産飼料100%でのおいしい牛乳生産技術の開発」
    「革新的技術開発・緊急展開事業（うち経営体強化プロジェクト）」
    Apr. 2017 - Mar. 2020
    三谷朋弘
    国立研究開発法人農業・食品産業技術総合研究機構生物系特定産業技術研究支援センター, Coinvestigator, Competitive research funding
  • 放牧牛乳・乳製品の特性を活かした高付加価値技術の開発 ①味覚センサ等による放牧牛乳の特徴付け
    平成２３年度農林水産省委託プロジェクト研究「自給飼料多給による高付加価値牛肉・牛乳生産技術の開発」
    2010 - 2012
    三谷朋弘
    農林水産省, Coinvestigator, Competitive research funding
  • Analysis of behavior of grazing cows by the movable body recognition and the analysis system using an automatic following system
    Grants-in-Aid for Scientific Research
    2009 - 2011
    NAKATSUJI Hiroki; KONDO Seiji; UEDA Koichiro; MITANI Tomohiro
    We tried to determine the moving locus of grazing cows by high resolution network cameras installed in pasture, which recorded the behavior of cows, the digital images were transmitted to a host personal computer by wireless LAN, and automatic image processing was made. As the result of trial and error, it was suggested to be possible to identify the place of cows grazed, depending on the conditions of cameras installed in pasture. Although the possibility of such a system configuration was recognized, the number of camera, and the improvement in accuracy of picture integrated processing with the host computer was remained as a future subject.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), 21580321
  • Study on occurrence factor and prevention against lipid oxidation of raw milk during preservation period
    Grants-in-Aid for Scientific Research
    2009 - 2011
    MITANI Tomohiro
    In dairy industry, a quality deterioration occurred by lipid oxidation of raw milk become a problem. This study objected to investigate an occurrence factor and prevention against lipid oxidation of raw milk during preservation period. Hexanal yielded at final step of lipid oxidation was produced from polyunsaturated fatty acids(PUFA) through a peroxide lipid. For milk containing a lot of highly-unsaturated fatty acid such as linolenic acid, hexanal was yielded at early preservation period. Conversely, for milk containing a lot of PUFA such as linoleic acid, hexanal was yielded 3 or 5 days after refrigerated storage. It is known that feeding regime for dairy cows strongly affected on fatty acid profile of milk. It would be considered usage and distribution of raw milk which is produced from different feeding regime.
    Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, Principal investigator, Competitive research funding, 21780241
■ Industrial Property Rights
  • 乳牛の飼養内容の予測方法、乳牛の飼料の選定方法、生乳の品質の制御方法
    Patent right, 大山孝治; 斉藤瑞恵; 三室明寛; 三谷朋弘
    特願2013-224038, 29 Oct. 2013
    特開2015-084677, 07 May 2015
  • 「身体活動促進剤」―ルテインを強化した乳とその有効成分を含むものー
    Patent right, 松本 恵; 矢島昌子; 三谷朋弘; 原 博; 矢島髙二
    特願2009-216482, 18 Sep. 2009
    特開2011-063552, 31 Mar. 2011

Research Profiles

■ Research Profiles