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Nakashima Taiken

Research Faculty of Agriculture　Fundamental AgriScience Research　Agrobiology and BioresourcesAssistant Professor

Researcher basic information

■ Degree
  • 博士（農学）, 九州大学
■ URL
researchmap URLホームページURL■ Various IDs
Researcher number
  • 70710945
J-Global ID■ Research Keywords and Fields
Research Keyword
  • 植物生体計測
  • Environmental stress
  • Photosynthesis
Research Field
  • Environmental Science/Agriculture Science, Agricultural environmental engineering and agricultural information engineering
  • Environmental Science/Agriculture Science, Crop production science
■ Educational Organization

Career

■ Career
Career
  • Sep. 2015 - Present
    Hokkaido University, Graduate School of Agriculture Research Faculty of Agriculture, 助教
  • Apr. 2013 - Sep. 2015
    Ehime University, Faculty of Agriculture, 特定研究員
  • Apr. 2010 - Mar. 2013
    日本学術振興会特別研究員
Educational Background
  • Mar. 2010 - Apr. 2013, 九州大学大学院, 生物資源環境科学府, 資源生物科学科
  • Apr. 2008 - Mar. 2010, 九州大学大学院, 生物資源環境科学府, 植物資源科学科
  • Oct. 2006 - Mar. 2008, 九州大学大学院, 生物資源環境科学府, 植物資源科学科
  • Mar. 2003 - Sep. 2006, オークランド大学, 理学部, 生物学科

Research activity information

■ Awards
  • Sep. 2013, 日本生物環境工学会, 論文奨励賞
    中島 大賢
  • Sep. 2012, 日本生物環境工学会, 優秀ポスター発表賞
    中島 大賢
  • Mar. 2012, 日本作物学会, 優秀発表賞
    中島 大賢
■ Papers
■ Other Activities and Achievements
■ Lectures, oral presentations, etc.
  • Evaluation of Hokkaido rice varieties on the canopy structure in light interception and nitrogen distributions for yield improvements
    Kabasawa T; Nishitsuji K; Chen Y; Horikoshi M; Nishimura T; Sato H; Nakashima T; Ichikawa S; Kashiwagi J
    Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 2021, Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, Japanese
    2021 - 2021
  • Varietal differences in post-silking kernel development and dry-down in maize (Zea mays L.)
    Yamaguchi Hiroto; Ishiyama Shiho; Matsui Yasuma; Ichikawa Shinji; Kashiwagi Junichi; Yamada Toshihiko; Nakashima Taiken
    Abstracts of Meeting of the CSSJ, 2021, CROP SCIENCE SOCIETY OF JAPAN, Japanese
    2021 - 2021
  • Comparative analysis on early growth vigor in maize (Zea mays L.) hybrids in different maturity ranges
    Ishiyama Shiho; Yamaguchi Hiroto; Kono Ryugo; Matsui Yasuma; Ichikawa Shinji; Kashiwagi Junichi; Yamada Toshihiko; Nakashima Taiken
    Abstracts of Meeting of the CSSJ, 2021, CROP SCIENCE SOCIETY OF JAPAN, Japanese
    2021 - 2021
  • Prolificacy accelerates kernel development and dry-down rate in maize (Zea mays L.)
    Yamaguchi Hiroto; Ishiyama Shiho; Matsui Yasuma; Ichikawa Shinji; Kashiwagi Junichi; Yamada Toshihiko; Nakashima Taiken
    Abstracts of Meeting of the CSSJ, 2021, CROP SCIENCE SOCIETY OF JAPAN, Japanese
    2021 - 2021
  • On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice.
    Hiroshi Wada; Yuto Hatakeyama; Taiken Nakashima; Hiroshi Nonami; Rosa Erra-Balsells; Makoto Hakata; Keisuke Nakata; Kenzo Hiraoka; Yayoi Onda; Hiroshi Nakano
    Scientific reports, 06 Feb. 2020, English
    06 Feb. 2020 - 06 Feb. 2020, Although a loss of healthy pollen grains induced by metabolic heat responses has been indicated to be a major cause of heat-induced spikelet sterility under global climate change, to date detailed information at pollen level has been lacking due to the technical limitations. In this study, we used picolitre pressure-probe-electrospray-ionization mass spectrometry (picoPPESI-MS) to directly determine the metabolites in heat-treated single mature pollen grains in two cultivars, heat-tolerant cultivar, N22 and heat-sensitive cultivar, Koshihikari. Heat-induced spikelet fertility in N22 and Koshihikari was 90.0% and 46.8%, respectively. While no treatment difference in in vitro pollen viability was observed in each cultivar, contrasting varietal differences in phosphatidylinositol (PI)(34:3) have been detected in mature pollen, together with other 106 metabolites. Greater PI content was detected in N22 pollen regardless of the treatment, but not for Koshihikari pollen. In contrast, there was little detection for phosphoinositide in the single mature pollen grains in both cultivars. Our findings indicate that picoPPESI-MS analysis can efficiently identify the metabolites in intact single pollen. Since PI is a precursor of phosphoinositide that induces multiple signaling for pollen germination and tube growth, the active synthesis of PI(34:3) prior to germination may be closely associated with sustaining spikelet fertility even at high temperatures.
  • Effects of seedling age on the growth of Hokkaido rice varieties in wide spacing cultivation
    Muraoka Takamichi; Kikuchi Toshiki; Nshitsuji Kosuke; Nagashima Reiko; Ichikawa Shinji; Nishimura Tsutomu; Nakashima Taiken; Junichi Kashiwagi
    Abstracts of Meeting of the CSSJ, 2020, CROP SCIENCE SOCIETY OF JAPAN, Japanese
    2020 - 2020
  • Effects of limiting sub-apical ears development on grain-filling and kernel dry-down processes in prolific maize hybrid
    Yamaguchi H; Nakashima T; Matsui Y; Ishiyama S; Ito K; Kono R; Hattori Y; Ichikawa S; Kashiwagi J; Yamada T
    Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 2020, Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, Japanese
    2020 - 2020
  • Effects of basal application of coated slow-release fertilizer and topdressing of urea on growth and grain yield of maize hybrids
    Matsui Y; Kashiwagi J; Yamada T; Nakashima T; Yamaguchi H; Hattori Y; Kono R; Ishiyama S; Ito K; Ichikawa S; Takemura K; Shinohara Y
    Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 2020, Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, Japanese
    2020 - 2020
  • Varietal differences in traits related to grain filling and drying down in maize hybrids with different maturity
    Yamaguchi H; Ishiyama S; Oki S; Okamoto T; Hattori Y; Ichikawa S; Kashiwagi J; Yamada T; Nakashima T
    Report of the Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, 2019, Hokkaido Branch, the Japanese Society of Breeding and Hokkaido Branch, the Crop Science Society of Japan, Japanese
    2019 - 2019
  • Potential use of late-maturing maize varieties for improving grain yieldand root-lodging resistance in Hokkaido
    Yamaguchi Hiroto; Oki Shio; Okamoto Tatsuro; Hattori Yoko; Ichikawa Shinji; Kashiwagi Junichi; Nakashima Taiken
    Abstracts of Meeting of the CSSJ, 2019, CROP SCIENCE SOCIETY OF JAPAN, Japanese
    2019 - 2019
  • Importance of ear as an alternative source for drought yield in wheat
    Nakayama Suzu; Yoshioka Yuichiro; Inoue Yoshiko; Ichikawa Shinji; Nakashima Taiken; Kashiwagi Junichi; An Ping
    Abstracts of Meeting of the CSSJ, 2019, CROP SCIENCE SOCIETY OF JAPAN, Japanese
    2019 - 2019
  • Improvement of Seedling Establishment by Seed Coating with Iron in Early-winter Direct-sowing Rice
    Satoko Oikawa; Masayoshi Nishi; Susumu Yui; Junichi Kashiwagi; Taiken Nakashima; Shinji Ichikawa; Toshiyuki Kimura; Youichi Ohdaira; Kiyoshi Nagasuga; Eiki Kuroda; Maya Matsunami; Hiroyuki Shimono
    Japanese Society of Crop Science, 2019, CROP SCIENCE SOCIETY OF JAPAN, Japanese
    2019 - 2019,

    Poor seedling establishment is a major problem for Early-Winter Direct-Sowing cultivation in rice. We examined the effects of seed coating with three materials (iron, calcium peroxide, starch) on the seedling establishment. The seedling establishment rate was only 1～3% in the control without seed coating in both 2016/2017 and 2017/2018 seasons in Iwate Prefecture. Only the coating with iron showed a significant increase in the seedling establishment rate, that is, 24% in the 2016/2017 season and 11～30% in the 2017/2018 season. Significant improvement of seedling establishment by seed coating with iron was confirmed similarly in additional four Prefectures (Hokkaido, Aomori, Akita and Mie) in 2017/2018 season. We concluded that the seed coating with iron is a promising methodology for improving seedling establishment for Early-Winter Direct-Sowing cultivation in rice.

  • 子実用トウモロコシに対する葉位別摘葉処理が収量および収量関連形質に及ぼす影響
    長岡詩帆; 土井希晃; 宋粮; 中島大賢; 佐藤智宏; 村井祐介; 義平大樹
    日本育種学会・日本作物学会北海道談話会年次講演会, Dec. 2017
  • 北海道水稲品種におけるソース能の経時変化が収量安定性に及ぼす影響
    若林侑; 竹澤広基; 中島大賢; 西村努; 道満剛平; 木下雅文; 市川伸次; 柏木純一
    日本育種学会・日本作物学会北海道談話会年次講演会, Dec. 2017
  • 北海道水稲多収品種における葉位別光合成速度と収量の関係
    竹澤 広基; 若林 侑; 菊地俊希; 中島 大賢; 西村 努; 市川 伸次; 柏木 純一
    日本育種学会・日本作物学会北海道談話会年次講演会, Dec. 2017
  • Phosphoinositides in Rice Pollen Grains Associated with Spikelet Fertility
    Hiroshi Wada; Taiken Nakashima; Makoto Hakata; Yuto Hatakeyama; Keisuke Nakata; Hiroshi Nonami; Rosa Erra-Balsells; Kenzo Hiraoka; Hiroshi Nakano
    2017 CIGR World Workshop, Sep. 2017
  • Nitrogen application reverses heat-induced rice chalkiness: Evidence for organelle rearrangement due to the recovery of protein synthesis in endosperm cells
    Yuto Hatakeyama; Yayoi Onda; Hiroshi Nonami; Taiken Nakashima; Hiroshi Nakano; Rosa Erra-Balsells; Kenzo Hiraoka; Hiroshi Wada
    Society for Experimental Biology Annual Meeting, Jun. 2017
  • Development of the on-site live cell metabolomics performable in controlled environment
    Hiroshi Wada; Hiroshi Nonami; Katsunori Tanaka; Taiken Nakashima; Yuto Hatakeyama; Hiroshi Nakano; Yayoi Onda; Kenzo Hiraoka; Makoto Hakata; Satoshi Morita
    Society for Experimental Biology Annual Meeting, Jun. 2017
  • 北海道で育成された水稲多収品種における多収要因の解析
    竹澤 広基; 若林 侑; 中島 大賢; 西村 努; 道満 剛平; 木下 雅文; 市川 伸次; 柏木 純一
    第243回日本作物学会講演会, Mar. 2016
  • リアルタイム・1細胞代謝物質計測のためのプレッシャープローブ・エレクトロスプレーイオン化
    野並浩; 中島大賢; 和田博史; 森田敏; 武森信暁; 武森文子; Erra-Balsells Rosa; 平岡賢三
    Sep. 2015
    [Invited]
  • 1細胞代謝物質・タンパク質計測のためのプレッシャープローブ・エレクトロスプレーイオン化
    中島大賢; 和田博史; 森田敏; 武森信暁; 武森文子; Erra-Balsells Rosa; 平岡賢三; 野並浩
    第63回質量分析総合討論会, Jun. 2015
    [Invited]
  • 細胞生体計測法を用いたトマトトライコームのEC濃度変化に対する生理応答の経過時的評価
    中田佳佑; 中島大賢; 田中晴規; 野並浩
    日本生物環境工学会, Sep. 2014
  • プレッシャープローブ・探針エレクトロスプレーイオン化を用いたタンパク質および代謝物質の超微量分析
    中島大賢; 武森信暁; 武森文子; Erra-Balsells Rosa; 平岡賢三; 野並浩
    第41回BMSコンファレンス, Jul. 2014
    [Invited]
  • Comparative metabolite profiling of intact tomato trichomes at single-cell resolution using pressure probe electrospray ionization mass spectrometry
    中島大賢; 春日純; 和田博史; 森田敏; Erra-Balsells Rosa; 平岡賢三; Gholipour Yousef; 野並浩
    第62回質量分析学会, May 2014
  • アリ科女王の受精嚢内精子の運動とその制御因子の探索
    後藤彩子; 野並浩; 春日純; 中島大賢; 小林悟; 伊藤文紀; 辻和希
    第58回日本応用動物昆虫学会, Mar. 2014
  • トウモロコシと半陰生C4植物コササキビの光合成特性に及ぼす生育光の影響
    玉垣和子; 中島大賢; 上野修
    第236回日本作物学会講演会, Sep. 2013
  • アマランサス属(NAD-ME型C4)における光合成能の種間差とその制御要因の検討
    堤伸子; 遠矢みゆき; 中島大賢; 上野修
    第233回日本作物学会講演会, Mar. 2012
  • 葉内ベタシアニンがアマランサス葉内部における光阻害特性に及ぼす影響-LIF測定法を用いた細胞レベルでの評価-
    中島大賢; 安河内裕司; 山下昭二; 荒木卓哉; 上野修
    第233回日本作物学会講演会, Mar. 2012
  • 葉内ベタシアニンがアマランサス葉の老化過程における光合成特性に及ぼす影響
    中島大賢; 荒木卓哉; 上野修
    第233回日本作物学会講演会, Mar. 2012
  • 異なるアマランサス種における葉内ベタシアニンの光防御機能の検討
    中島大賢; 安河内裕司; 山下昭二; 上野修
    第233回日本作物学会講演会, Mar. 2012
  • アマランサス葉の老化に伴う光合成能の低下は向軸側と背軸側で異なる
    中島大賢; 堤伸子; 荒木卓哉; 上野修
    第233回日本作物学会講演会, Mar. 2012
  • ヒユ属（Amaranthus）C4植物における光合成能の変異とその制御要因の解析
    堤伸子; 中島大賢; 上野修
    第232回日本作物学会講演会, Sep. 2011
  • アマランサスにおけるベタシアニン蓄積が長期乾燥下の光阻害並びに光合成に及ぼす影響
    中島大賢; 荒木卓哉; 上野修
    第231回日本作物学会講演会, Mar. 2011
  • Laser-Induced Fluorescence測定によるC4植物の葉細胞における光阻害特性評価法の検討
    中島大賢; 山下昭二; 安河内裕司; 荒木卓哉; 上野修
    第231回日本作物学会講演会, Mar. 2011
  • Photoprotective function of foliar betacyanin in leaves of Amaranthus cruentus L. under drought stress
    Taiken Nakashima; Takuya Araki; Osamu Ueno
    Aug. 2010
  • 葉内ベタシアニンが乾燥条件下のアマランサスにおける光阻害に及ぼす影響
    中島大賢; 荒木卓哉; 上野修
    第229回日本作物学会講演会, Mar. 2010
  • アマランサスにおける葉内ベタシアニンの光阻害緩和作用の検討
    中島大賢; 荒木卓哉; 上野修
    Sep. 2008
  • Variations in responses of leaf photosynthesis and dry matter productivity to the different levels of nitrogen application in Amaranthus spp
    Taiken Nakashima; Takuya Araki
    May 2008
■ Syllabus
  • 大学院共通授業科目（一般科目）：自然科学・応用科学, 2024年, 修士課程, 大学院共通科目
  • 一般教育演習(ﾌﾚｯｼｭﾏﾝｾﾐﾅｰ), 2024年, 学士課程, 全学教育
  • 生物資源科学特講, 2024年, 学士課程, 農学部
  • 生物資源科学実験Ⅰ, 2024年, 学士課程, 農学部
■ Affiliated academic society
  • JAPANESE SOCIETY OF AGRICULTURAL, BIOLOGICAL AND ENVIRONMENTAL ENGINEERS AND SCIENTISTS
  • CROP SCIENCE SOCIETY OF JAPAN
  • 日本草地学会
■ Research Themes
  • Kinetic study for synchronization nutrients requirement of Crop production and nutrients supply from cover crop residues.
    Grants-in-Aid for Scientific Research
    01 Apr. 2022 - 31 Mar. 2025
    平田 聡之; 中島 大賢
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 22K05925
  • Redesigning ideal crop canopy architecture using drone-based phenotyping techniques
    Grants-in-Aid for Scientific Research
    01 Apr. 2021 - 31 Mar. 2024
    中島 大賢; 加藤 洋一郎; 郭 威
    初年度はまず，収量特性が異なる茎葉型および子実型の2品種を北海道大学と東京大学の2地点で栽培した．北海道大学では，ドローン画像および群落内部の仰視画像を経時的に取得し，登熟初期の地上部構造特性を個体・群落規模で調査した．構造解析には投影法および層別刈り取り法を用い，各節の個葉形態や葉身傾斜角度および雌穂着生位置など，個体の草型を決定する形態形質を評価した．加えて，群落内の葉面積分布と光透過特性などの基本的な群落構造特性を解析した．東京大学では，ドローンおよびローバー型地上走行ロボットに搭載した各種センサーを用いて群落表層および内部の構造情報を経時計測し，画像解析や3次元点群再構築による形態評価手法の開発を行った．
    構造解析の結果，個体および群落の構造特性には顕著な品種間差異が認められた．茎葉型品種では群落上層の葉面積が多く，これは上位節間が狭く，中位葉の葉身傾斜角度が大きいことに起因していた．子実型品種では個体あたりの葉面積は少ないものの，上位節間が長いことに加え，着雌穂葉の葉身傾斜角度が有意に小さいことにより，子実充填への寄与が大きい着雌穂葉の光捕集効率が高まったと考えられた．また，雌穂着生位置が茎葉型品種に比べて有意に低く，地上部の重心高を低下させることで耐倒伏性の向上に寄与していた．以上より，本試験で供試した子実型品種では，収量性と耐倒伏性の両立に有利な形態特性を備えていることが示唆された．今後は，このような形態形質の品種間差異について新規解析技術の応用を試みる．本年度までに，トウモロコシ幼植物体の3次元再構築やドローン計測による群落表層構造の時系列観測については基礎データが既に得られている．一方で，ドローン計測による評価が困難な群落内部の構造解析についてはさらに改良を進める必要がある．
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 21K05537
  • International research network for the improvement of direct-seeded rice systems in stress-prone environments in tropical Asia
    Grants-in-Aid for Scientific Research
    09 Oct. 2018 - 31 Mar. 2023
    加藤 洋一郎; 中島 大賢; 深野 祐也; 藤田 大輔
    コロナ禍において、熱帯アジアではロックダウンによる厳しい外出制限がかけられている地域もある。オンラインによる共同研究展開には限界があり、不良環境の直播稲作地帯への現地調査や現地試験に制限が生じた状況は大きく改善されなかった。そのような中、国際共同研究ネットワーク構築と強化をオンラインベースで進めることにエフォートを割いた。ACSAC10（アジア作物学会国際会議；オンライン開催）において、本研究プロジェクトによって構築した直播稲作研究ネットワークを活用して、中国・オーストラリア・インド・フィリピン、そして日本の５カ国からの研究者を招待し、アジア・オセアニア圏の直播稲作の研究課題と技術革新、そして社会実装に向けた研究課題についてセッションをオーガナイズし、議論を行った。セッションにはミャンマー、インドネシア他熱帯アジアからの参加もあり、のちに多くのフィードバックを得て、直播稲作の国際的な研究基盤をさらに強化することが出来た。また、熱帯アジアにおける直播水稲栽培の現地共同研究を一部で再開し、東ティモール国において展開される水稲直播栽培の実態と生産課題の調査、生産力調査を行った（当該国において直播稲作の栽培研究が実施されるのは初めてである）。
    Japan Society for the Promotion of Science, Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), The University of Tokyo, 18KK0169
  • Evaluation of potential function of non-photochemical quenching as a heat source to chloroplast under chilling stress
    Grants-in-Aid for Scientific Research
    01 Apr. 2018 - 31 Mar. 2021
    Nakashima Taiken
    Short growing season is a major limitation on maize production in high latitude cold regions, leading to high density planting which poses an increased risk of root lodging. In this project, therefore, a comparative study on varietal differences in photosynthetic responses to early stage chilling stress was conducted to seek potential use of late-maturing varieties with improved cold tolerance. Among 18 varieties examined, two hybrids differing in early vigor and photosynthetic traits were identified. Further comparison of these hybrids revealed the importance of higher capacity of non-photochemical quenching (NPQ), as well as of other photosynthetic components.As an additional and potential function of NPQ, effects of heat dissipated through NPQ processes on chloroplast temperature were also evaluated by utilizing a microscope spectroscopy which enables simultaneous monitoring of chlorophyll fluorescence quenching and thermoresponsive fluorescence probe.
    Japan Society for the Promotion of Science, Grant-in-Aid for Early-Career Scientists, Hokkaido University, 18K14450
  • Development of drought tolerant energy crops based on field researches in the North American arid areas
    Grants-in-Aid for Scientific Research
    01 Apr. 2017 - 31 Mar. 2020
    YAMADA Toshihiko
    Non-food energy crops such as Miscanthus spp. have been taken attention as one of feedstocks for renewable energy such as biofuels. They are required to be cultivated in marginal agriculture lands where environmental stresses such as droughts occur. At the two sites in Japan and the United States, we evaluated the drought tolerance and measured photosynthetic characteristics using various strains of Miscanthus spp. Genotypes with excellent drought tolerance were almost consistent in both sites experiments, and Miscanthus sinensis “PMS-285” from the Yangtze-Qinling Mountains region showed excellent drought tolerance and high photosynthetic ability even in drought condition. In addition, regarding Miscane (hybrids between Miscanthus and sugarcane), the photosynthetic ability at low temperature, which is related to drought tolerance, is intermediate between both parents. This finding reveals that Miscanthus could contributes to improve environmental adaptability of sugarcane.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 17H04615
  • Cell dynamics of heat-induced rice chalky formation and nitrogen-enhanced suppression unveiled by using newly-developed on-site cell-specific analysis
    Grants-in-Aid for Scientific Research
    01 Apr. 2016 - 31 Mar. 2020
    Wada Hiroshi
    Heat-induced chalky formation is a major concern in rice production. The chalkiness is suppressed by nitrogen, yet little is known about the cellular dynamics. Picolitre pressure-probe electrospray-ionization mass spectrometry and transmission electron microscopy were used to investigate the cellular mechanisms of both processes in single endosperm cells of intact growing plants. We found an enlargement of protein storage vacuoles in osmotically adjusted cells under heat conditions with little accumulation of storage proteins prior to chalky formation, in addition to the partial inhibition of amyloplast development. Increased nitrogen availability enhanced heat tolerance through strong osmotic adjustment and the cells sustained protein synthesis even under heat conditions, which led to normal storage protein and amyloplast development to suppress air space formation. These findings provide new insight into the nitrogen-associated heat responses in the growing endosperm cells.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (A), National Agriculture and Food Research Organization, 16H02533
  • Speaking Cell Approach by on-site/real-time cellular and molecular measurements
    Grants-in-Aid for Scientific Research
    31 May 2012 - 31 Mar. 2017
    NONAMI Hiroshi; MORITA Satoshi; WADA Hiroshi; NAKASHIMA Taiken
    By using physiological molecular information, environmental conditions can be adjusted optimally to grow crops in plant growth factories. Such a control method uses physiological information to optimize energy efficiency and product quality control in plant growth factories, and is known as the “speaking cell approach” (SCA). In the present study, methods for on-site/real time cellular and molecular measurement techniques have been developed by using a cell pressure probe and probe electrospray ionization mass spectrometry for SCA. By mixing ionic liquid with the silicone oil in the pressure probe, pico pressure probe electrospray ionization mass spectrometry was developed to measure metabolite concentrations of single cells of plants. Hence, the water status and metabolite concentrations of single cells of plants can be measured simultaneously to evaluate the physiological conditions in real time to control environmental conditions in greenhouses.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (S), Ehime University, 24228004