Researcher basic information

• Doctor of Life Science, Hokkaido University, Mar. 2023

• https://researchmap.jp/shuya_ohira?lang=en

• https://orcid.org/0000-0003-4326-9707
• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=202301011646046440

• https://orcid.org/0000-0003-4326-9707

• 202301011646046440

• 粘膜免疫
• 腸内環境
• 腸内細菌叢
• 自然免疫
• α-defensin
• Paneth細胞
• Noncommunicable diseases

• Life Science, Immunology

Research activity information

• Modulation of Bifidobacterium by HD5 during weaning is associated with high abundance in later life
  Yu Shimizu; Yuki Yokoi; Shuya Ohira; Hirohisa Izumi; Satomi Kawakami; Miu Ihara; Fuka Tabata; Yasuhiro Takeda; Takashi Kimura; Koshi Nakamura; Akiko Tamakoshi; Tokiyoshi Ayabe; Kiminori Nakamura
  Communications Medicine, 01 Jul. 2025
  Scientific journal
• Potential consequences of phototoxicity on cell function during live imaging of intestinal organoids
  Yuki Yokoi; Ryu Nakamura; Shuya Ohira; Shota Takemi; Tokiyoshi Ayabe; Kiminori Nakamura
  PLOS ONE, 19, 11, e0313213, e0313213, Public Library of Science (PLoS), 15 Nov. 2024
  Scientific journal, Live imaging visualizes the structure, dynamics, and function of cells and tissues to reveal the molecular mechanisms, and has contributed to the advancement of life science. In live imaging, it has been well known that there is a trade-off between higher-resolution analysis and cell damage caused by light illumination, i.e., phototoxicity. However, despite the risk of unknowingly distorting experimental results, phototoxicity is an unresolved issue in live imaging because overall consequences occurring inside cells due to phototoxicity remains unknown. Here, we determined the molecular process of phototoxicity-induced cell damage systematically under low- and high-dose light illumination conditions by analyzing differential gene expression using RNA-sequencing in a three-dimensional organoid of small intestinal epithelial cells, enteroid. The low-dose light illumination already induced various abnormalities in functional molecules involved in the response to reactive oxygen species generated by the excitation of fluorescent dyes, intracellular metabolism, mitosis, immune responses, etc., at mRNA expression level. Together with the behavior toward apoptosis caused by high-dose light illumination, the light dose-dependent progression of intracellular damage was revealed. About visible impairment of intestinal epithelial function, failures in both the structure-forming ability of enteroids and Paneth cell granule secretion were observed under high-dose light illumination, while the drug efflux was not disturbed despite abnormal drug efflux transporter mRNA expression. Based on the gene expression profiles, we comprehensively clarified phenomena in the cells at mRNA level that cannot be recognized both morphologically and functionally during live imaging, further providing a new insight into the risk of phototoxicity. This study warns from the aspect of mRNA expression that awareness of phototoxic artifacts is needed when analyzing cellular function and the mechanism in live imaging.
• Decreased Paneth cell α-defensins promote fibrosis in a choline-deficient L-amino acid-defined high-fat diet-induced mouse model of nonalcoholic steatohepatitis via disrupting intestinal microbiota
  Shunta Nakamura; Kiminori Nakamura; Yuki Yokoi; Yu Shimizu; Shuya Ohira; Mizu Hagiwara; Zihao Song; Li Gan; Tomoyasu Aizawa; Daigo Hashimoto; Takanori Teshima; Andre J. Ouellette; Tokiyoshi Ayabe
  Scientific Reports, 13, 1, Springer Science and Business Media {LLC}, 09 Mar. 2023
  English, Scientific journal, AbstractNonalcoholic steatohepatitis (NASH) is a chronic liver disease characterized by fibrosis that develops from fatty liver. Disruption of intestinal microbiota homeostasis, dysbiosis, is associated with fibrosis development in NASH. An antimicrobial peptide α-defensin secreted by Paneth cells in the small intestine is known to regulate composition of the intestinal microbiota. However, involvement of α-defensin in NASH remains unknown. Here, we show that in diet-induced NASH model mice, decrease of fecal α-defensin along with dysbiosis occurs before NASH onset. When α-defensin levels in the intestinal lumen are restored by intravenous administration of R-Spondin1 to induce Paneth cell regeneration or by oral administration of α-defensins, liver fibrosis is ameliorated with dissolving dysbiosis. Furthermore, R-Spondin1 and α-defensin improved liver pathologies together with different features in the intestinal microbiota. These results indicate that decreased α-defensin secretion induces liver fibrosis through dysbiosis, further suggesting Paneth cell α-defensin as a potential therapeutic target for NASH.
  
• Efficient and simple genetic engineering of enteroids using mouse isolated crypts for investigating intestinal functions
  Shuya Ohira; Yuki Yokoi; Tokiyoshi Ayabe; Kiminori Nakamura
  Biochemical and Biophysical Research Communications, Dec. 2022
  Scientific journal
• Intestinal commensal microbiota and cytokines regulate Fut2 + Paneth cells for gut defense
  Mariko Kamioka; Yoshiyuki Goto; Kiminori Nakamura; Yuki Yokoi; Rina Sugimoto; Shuya Ohira; Yosuke Kurashima; Shingo Umemoto; Shintaro Sato; Jun Kunisawa; Yu Takahashi; Steven E. Domino; Jean-Christophe Renauld; Susumu Nakae; Yoichiro Iwakura; Peter B. Ernst; Tokiyoshi Ayabe; Hiroshi Kiyono
  Proceedings of the National Academy of Sciences, 119, 3, Proceedings of the National Academy of Sciences, 18 Jan. 2022
  Scientific journal
• Decrease of α-defensin impairs intestinal metabolite homeostasis via dysbiosis in mouse chronic social defeat stress model
  Kosuke Suzuki; Kiminori Nakamura; Yu Shimizu; Yuki Yokoi; Shuya Ohira; Mizu Hagiwara; Yi Wang; Yuchi Song; Tomoyasu Aizawa; Tokiyoshi Ayabe
  Scientific Reports, 11, 1, Springer Science and Business Media {LLC}, Dec. 2021
  English, Scientific journal, AbstractPsychological stress has been reported to relate to dysbiosis, imbalance of the intestinal microbiota composition, and contribute to the onset and exacerbation of depression, though, underlying mechanisms of psychological stress-related dysbiosis have been unknown. It has been previously established that α-defensins, which are effector peptides of innate enteric immunity produced by Paneth cells in the small intestine, play an important role in regulation of the intestinal microbiota. However, the relationship between disruption of intestinal ecosystem and α-defensin under psychological stress is yet to be determined. Here we show using chronic social defeat stress (CSDS), a mouse depression model that (1) the exposure to CSDS significantly reduces α-defensin secretion by Paneth cells and (2) induces dysbiosis and significant composition changes in the intestinal metabolites. Furthermore, (3) they are recovered by administration of α-defensin. These results indicate that α-defensin plays an important role in maintaining homeostasis of the intestinal ecosystem under psychological stress, providing novel insights into the onset mechanism of stress-induced depression, and may further contribute to discovery of treatment targets for depression.
• Paneth cell α-defensin misfolding correlates with dysbiosis and ileitis in Crohn’s disease model mice
  Yu Shimizu; Kiminori Nakamura; Aki Yoshii; Yuki Yokoi; Mani Kikuchi; Ryuga Shinozaki; Shunta Nakamura; Shuya Ohira; Rina Sugimoto; Tokiyoshi Ayabe
  Life Science Alliance, 3, 6, Life Science Alliance, {LLC}, Jun. 2020
  Scientific journal
• Expression and Localization of Paneth Cells and Their α-Defensins in the Small Intestine of Adult Mouse.
  Kiminori Nakamura; Yuki Yokoi; Rie Fukaya; Shuya Ohira; Ryuga Shinozaki; Takuto Nishida; Mani Kikuchi; Tokiyoshi Ayabe
  Frontiers in immunology, 11, 570296, 570296, 2020, [International Magazine]
  English, Scientific journal, Paneth cells contribute to intestinal innate immunity by sensing bacteria and secreting α-defensin. In Institute of Cancer Research (ICR) mice, α-defensin termed cryptdin (Crp) in Paneth cells consists of six major isoforms, Crp1 to 6. Despite accumulating evidences that α-defensin functions in controlling the intestinal microbiota, topographical localization of Paneth cells in the small intestine in relation to functions of α-defensin remains to be determined. In this study, we examined the expression level of messenger RNA (mRNA) encoding six Crp-isoforms and Crp immunoreactivities using singly isolated crypts together with bactericidal activities of Paneth cell secretions from isolated crypts of duodenum, jejunum, and ileum. Here we showed that levels of Crp mRNAs in the single crypt ranged from 5 x 103 to 1 x 106 copies per 5 ng RNA. For each Crp isoform, the expression level in ileum was 4 to 50 times higher than that in duodenum and jejunum. Furthermore, immunohistochemical analysis of isolated crypts revealed that the average number of Paneth cell per crypt in the small intestine increased from proximal to distal, three to seven-fold, respectively. Both Crp1 and 4 expressed greater in ileal Paneth cells than those in duodenum or jejunum. Bactericidal activities in secretions of ileal Paneth cell exposed to bacteria were significantly higher than those of duodenum or jejunum. In germ-free mice, Crp expression in each site of the small intestine was attenuated and bactericidal activities released by ileal Paneth cells were decreased compared to those in conventional mice. Taken together, Paneth cells and their α-defensin in adult mouse appeared to be regulated topographically in innate immunity to control intestinal integrity.
• Butyric Acid and Leucine Induce α-Defensin Secretion from Small Intestinal Paneth Cells.
  Akiko Takakuwa; Kiminori Nakamura; Mani Kikuchi; Rina Sugimoto; Shuya Ohira; Yuki Yokoi; Tokiyoshi Ayabe
  Nutrients, 11, 11, 18 Nov. 2019, [International Magazine]
  English, Scientific journal, The intestine not only plays a role in fundamental processes in digestion and nutrient absorption, but it also has a role in eliminating ingested pathogenic bacteria and viruses. Paneth cells, which reside at the base of small intestinal crypts, secrete α-defensins and contribute to enteric innate immunity through potent microbicidal activities. However, the relationship between food factors and the innate immune functions of Paneth cells remains unknown. Here, we examined whether short-chain fatty acids and amino acids induce α-defensin secretion from Paneth cells in the isolated crypts of small intestine. Butyric acid and leucine elicit α-defensin secretion by Paneth cells, which kills Salmonella typhimurium. We further measured Paneth cell secretion in response to butyric acid and leucine using enteroids, a three-dimensional ex vivo culture system of small intestinal epithelial cells. Paneth cells expressed short-chain fatty acid receptors, Gpr41, Gpr43, and Gpr109a mRNAs for butyric acid, and amino acid transporter Slc7a8 mRNA for leucine. Antagonists of Gpr41 and Slc7a8 inhibited granule secretion by Paneth cells, indicating that these receptor and transporter on Paneth cells induce granule secretion. Our findings suggest that Paneth cells may contribute to intestinal homeostasis by secreting α-defensins in response to certain nutrients or metabolites.

• Paneth細胞の機能異常は腸内細菌叢の破綻を介してNASH発症に関与する
  中村 駿太; 中村 公則; 菊池 摩仁; 横井 友樹; 大平 修也; 清水 由宇; 西田 琢人; 綾部 時芳, 腸内細菌学雑誌, 34, 2, 101, 101, Apr. 2020
  (公財)腸内細菌学会, Japanese
• Enteroid内腔へのマイクロインジェクションによるPaneth細胞顆粒分泌評価システムの確立と応用
  米田 司; 中村 公則; 横井 友樹; 大平 修也; 菊池 摩仁; 櫻木 直也; 綾部 時芳, 生命科学系学会合同年次大会, 2017年度, [1P, 1073], Dec. 2017
  生命科学系学会合同年次大会運営事務局, Japanese

• The effect of social isolation stress-induced Paneth cell abnormality on Crohn's disease via dysbiosis
  Miu IHARA; Shuya OHIRA; Yuki YOKOI; Yuna ABE; Kanta HARASAWA; Kiminori NAKAMURA
  JSBBA 2026 Annual Meeting, 11 Mar. 2026, Japan Society for Bioscience, Biotechnology, and Agrochemistry, English, Oral presentation
  09 Mar. 2026 - 12 Mar. 2026, Kyoto city, Japan
• Intestinal microbiota disruption by psychological stress-induced Paneth cell damage is associated with depression-like behaviors
  Yuna ABE; Yuki YOKOI; Miyuki IKEDA; Kanta HARASAWA; Miu IHARA; Shuya OHIRA; Kiminori NAKAMURA
  The 48th Annual Meeting of the Molecular Biology Society of Japan, 04 Dec. 2025, 日本分子生物学会, English, Poster presentation
  03 Dec. 2025 - 05 Dec. 2025, 横浜市, Japan
• Effect of the intestinal microbiota regulation by Paneth cell α-defensin on food allergy development
  Kanta HARASAWA; Shuya OHIRA; Miu IHARA; Yuna ABE; Yuki YOKOI; Kiminori NAKAMURA
  The 48th Annual Meeting of the Molecular Biology Society of Japan, 04 Dec. 2025, 日本分子生物学会, English, Poster presentation
  03 Dec. 2025 - 05 Dec. 2025, 横浜市, Japan
• 精神的ストレスによるPaneth細胞α-defensin分泌低下は腸内細菌叢組成を攪乱し，抑うつ様行動に関与する
  横井友樹; 阿部悠和; 井原海詩; 原澤頑太; 大平修也; 中村公則
  第29回腸内細菌学会学術集会, 23 Jun. 2025, 腸内細菌学会, Japanese
  23 Jun. 2025 - 24 Jun. 2025, 江戸川区, Japan
• Human defensin 5 (HD5)の分泌と相関する離乳期のBifidobacterium占有率の高さが将来のBifidobacterium豊富な腸内細菌叢形成に関係する
  第29回腸内細菌学会学術集会, 23 Jun. 2025, 腸内細菌学会, Japanese
  23 Jun. 2025 - 24 Jun. 2025, 江戸川区, Japan
• 食成分が誘導するPaneth細胞α-defensin分泌応答の解明
  横井友樹; 原澤頑太; 井原海詩; 大平修也; 中村公則
  アミノ酸学会 第8回 産官学連携シンポジウム, 02 Jun. 2025, Japanese, Poster presentation
  02 Jun. 2025 - 02 Jun. 2025
• Paneth細胞α-defensinによる小腸粘膜免疫の制御メカニズム
  大平修也
  2024 自然免疫セミナー, 05 Dec. 2024, Japanese, Invited oral presentation
  05 Dec. 2024 - 05 Dec. 2024, 札幌, Japan, [Invited]
• 食物アレルギー発症における異常Paneth細胞の関与
  原澤頑太; 大平修也; 横井友樹; 竹見祥大; 井原海詩; 阿部悠和; 中村公則
  日本食品免疫学会 設立20周年記念学術大会 (JAFI2024), 02 Dec. 2024, 日本食品免疫学会, Japanese, Oral presentation
  02 Dec. 2024 - 03 Dec. 2024, 東京都文京区東京大学内, Japan
• The effect of psychological stress induced by social isolation on Crohn's disease relapse.
  井原海詩; 大平修也; 横井友樹; 竹見祥大; 阿部悠和; 原澤頑太; 松下稜; 中村公則
  The 47th Annual Meeting of the Molecular Biology Society of Japan, 28 Nov. 2024, 日本分子生物学会, English, Poster presentation
  26 Nov. 2024 - 29 Nov. 2024, 福岡, Japan
• Selective Paneth cell granule secretion in response to pathogenic and commensal bacteria in enteric mucosal immunity
  Yuki Yokoi; Shuya Ohira; Haruto Matsuoka; Shota Takemi; Tokiyoshi Ayabe; Kiminori Nakamura
  The 52nd Annual Meeting of the Japanese Society for Immunology, 18 Jan. 2024, English, Poster presentation
  17 Jan. 2024 - 19 Jan. 2024, 千葉, Japan
• 小腸上皮細胞機能の分子メカニズム解明に向けた高効率かつ簡便な遺伝子改変enteroidの作製法の確立
  松下稜; 横井友樹; 大平修也; 松岡温音; 竹見祥大; 中村公則
  第46回日本分子生物学会年会, 06 Dec. 2023, English, Poster presentation
  06 Dec. 2023 - 08 Dec. 2023, 神戸, Japan, [Domestic Conference]
• 病原体と共生菌に対する選択的なPaneth細胞顆粒分泌調節による自然免疫と共生のメカニズム
  横井友樹; 大平修也; 綾部時芳; 中村公則
  令和4年度北大細胞生物研究集会, 14 Mar. 2023, Japanese, Oral presentation
  14 Mar. 2023 - 14 Mar. 2023
• High-efficient genetic engineering of enteroids using mouse isolated crypts revealing the intestinal function
  大平修也; 横井友樹; 海堀唯人; 清水由宇; 綾部時芳; 中村公則
  第51回日本免疫学会学術集会, 08 Dec. 2022, English, Poster presentation
  07 Dec. 2022 - 09 Dec. 2022
• 腸管恒常性維持における病原菌と常在菌の選択的センシングによるPaneth細胞顆粒分泌調節
  横井友樹; 大平修也; 松岡温音; 海堀唯人; 篠崎竜我; 綾部時芳; 中村公則
  第45回日本分子生物学会年会, 02 Dec. 2022, English, Oral presentation
  30 Nov. 2022 - 02 Dec. 2022
• The mechanism of acetylcholine-induced Paneth cell secretory responses in innate enteric immunity
  横井友樹; 大平修也; 菊池摩仁; 綾部時芳; 中村公則
  第50回日本免疫学会学術集会, 08 Dec. 2021, English, Poster presentation
  08 Dec. 2021 - 10 Dec. 2021
• 単離陰窩を用いた高効率で簡便なtransgenic mouse enteroidの作製と応用
  大平修也; 横井友樹; 菊池摩仁; 八塚夏美; 綾部時芳; 中村公則
  第44回日本分子生物学会年会, 03 Dec. 2021, English, Oral presentation
  01 Dec. 2021 - 03 Dec. 2021
• 母親の高脂肪食摂取が子のPaneth細胞の発達に与える作用メカニズム
  八塚夏美; 杉本理菜; 大平修也; 横井友樹; 菊池摩仁; 綾部時芳; 中村公則
  第44回日本分子生物学会年会, 02 Dec. 2021, English, Poster presentation
  01 Dec. 2021 - 03 Dec. 2021
• ER stress-associated misfolding of Paneth cell a-defensin induces dysbiosis and ileitis in a murine model of Crohn’s disease
  Yu Shimizu; Kiminori Nakamura; Yoshii Aki; Yokoi Yuki; Kikuchi Mani; Shinozaki Ryuga; Nakamura Shunta; Shuya Ohira; Rina Sugimoto; Tokiyoshi Ayabe
  World Microbe Forum, 21 Jun. 2021, English, Poster presentation
  20 Jun. 2021 - 24 Jun. 2021
• Dynamics of Paneth cell secretory responses to bacteria in innate enteric immunity
  Yuki Yokoi; Kiminori Nakamura; Shuya Ohira; Ryuga Shinozaki; Mani Kikuchi; Tokiyoshi Ayabe
  World Microbe Forum, 21 Jun. 2021, English, Poster presentation
  21 Jun. 2021 - 21 Jun. 2021
• Paneth 細胞の機能異常は腸内細菌叢の破綻を介してNASH 発症に関与する
  中村駿太; 中村公則; 菊池摩仁; 横井友樹; 大平修也; 清水由宇; 西田琢人; 綾部時芳
  第24 回腸内細菌学会学術集会, Jun. 2020, English, Others
  Jun. 2020 - Jun. 2020
• Acetylcholine-induced Paneth cell granule secretion via muscarinic M3 receptor in mouse small intestine
  Yuki Yokoi; Kiminori Nakamura; Rina Sugimoto; Shuya Ohira; Mani Kikuchi; Tokiyoshi Ayabe
  ASCB|EMBO 2019 meeting, 10 Dec. 2019, English, Poster presentation
  07 Dec. 2019 - 11 Dec. 2019
• Paneth Cell Dysfunction In Pathophysiology Of Nonalcoholic Steatohepatitis
  Shunta Nakamura; Kiminori Nakamura; Mani Kikuchi; Yuki Yokoi; Shuya Ohira; Yu Shimizu; Takuto Nishida; Tokiyoshi Ayabe
  ASCB|EMBO 2019 meeting, 09 Dec. 2019, English, Poster presentation
  07 Dec. 2019 - 11 Dec. 2019
• Establishment of transgenic enteroid by gene transduction in isolated crypts of mouse small intestine
  Shuya Ohira
  Innate Immunity Research Seminar, 07 Oct. 2019, English, Oral presentation
  07 Oct. 2019 - 07 Oct. 2019
• Visualization and quantitation of Paneth cell granule secretory responses to bacteria using enteroids
  横井友樹; 中村公則; 米田司; 大平修也; 中村駿太; 菊池摩仁; 綾部時芳
  北海道大学ライフサイエンスフォーラム2019, 28 Sep. 2019, English, Poster presentation
  28 Sep. 2019 - 28 Sep. 2019
• Analysis of the regulation of Paneth cell differentiation in stem cell niche by using intestinal organoid
  大平修也; 中村公則; 菊池摩仁; 横井友樹; 杉本理菜; 綾部時芳
  北海道大学ライフサイエンスフォーラム2019, 28 Sep. 2019, English, Poster presentation
  28 Sep. 2019 - 28 Sep. 2019
• Dynamics and mechanisms of Paneth cell granule responses in enteroids
  Yuki Yokoi; Kiminori Nakamura; Shuya Ohira; Rina Sugimoto; Tokiyoshi Ayabe
  Hokkaido University-University of Southern California Research Workshop “Gut-Organ Crosstalk”, 20 May 2019, English, Invited oral presentation
  20 May 2019 - 20 May 2019
• Analysis of the regulation of Paneth cell differentiation in stem cell niche by using intestinal organoid
  大平修也; 中村公則; 菊池摩仁; 横井友樹; 杉本理菜; 綾部時芳
  第41回日本分子生物学会年会, 30 Nov. 2018, English, Poster presentation
  28 Nov. 2018 - 30 Nov. 2018
• Elucidation of a differentiation mechanism for the intestinal epithelium by Paneth cell
  Shuya Ohira
  Innate Immunity Research Seminar, 15 Oct. 2018, English, Oral presentation
  15 Oct. 2018 - 15 Oct. 2018
• Establishment of the evaluation system for Paneth cell granule secretion by using enteroid with lumen microinjection and its application
  米田司; 中村公則; 横井友樹; 大平修也; 菊池摩仁; 櫻木直也; 綾部時芳
  第40回日本分子生物学会年会, 06 Dec. 2017, English, Poster presentation
  06 Dec. 2017 - 09 Dec. 2017
• Uncovering the mechanism of differentiation by Paneth cell using intestinal organoids, Innate Immunity Research Seminar
  Shuya Ohira
  Innate Immunity Research Seminar, 16 Oct. 2017, English, Oral presentation
  16 Oct. 2017 - 16 Oct. 2017
• 腸管オルガノイドを用いたPaneth細胞分化制御機構の解明
  大平修也
  第3回腸内環境と免疫の融合 合同セミナー, 11 Sep. 2017, Japanese, Oral presentation
  11 Sep. 2017 - 11 Sep. 2017
• Enteroid 内腔へのmicroinjection によるPaneth 細胞の顆粒分泌システム
  菊池摩仁; 米田司; 横井友樹; 大平修也; 櫻木直也; 中村公則; 綾部時芳
  第12 回遺伝子栄養学研究会, 25 Aug. 2017, English
  25 Aug. 2017 - 25 Aug. 2017

• 大学院共通授業科目（一般科目）：複合領域, 2024年, 修士課程, 大学院共通科目

• Aug. 2024 - Present
  日本食品免疫学会
• Jul. 2018 - Present
  日本分子生物学会
• Oct. 2017 - Present
  日本免疫学会

• α-Defensinの共生制御が担う腸を起点とする粘膜免疫ネットワーク
  科学研究費助成事業
  Apr. 2022 - Mar. 2024
  大平 修也
  日本学術振興会, 特別研究員奨励費, 北海道大学, Principal investigator, 22KJ0054
• 小腸上皮細胞の環境因子との相互作用による生体恒常性維持機構の解明
  DX博士人材フェローシップ
  Oct. 2021 - Mar. 2022
  北海道大学, 挑戦的、「融合研究の場」実施支援事業, 北海道大学, Principal investigator, Competitive research funding, PMJSP2119
• 小腸上皮細胞の環境因子との相互作用による生体恒常性維持機構の解明
  DX博士人材フェローシップ
  Oct. 2021 - Mar. 2022
  北海道大学, 研究費, 北海道大学, Principal investigator, Competitive research funding, PMJSP2119

• 2023 Interdisciplinary Networking Trip for Doctoral Students
  17 Feb. 2024 - 18 Feb. 2024
  Planning etc
  Academic society etc
  東京外国語大学
  Inter-University Seminar House (Tokyo, Hachioji)

• ～負けるな小腸・がんばれ免疫～ 北海道大学☆はかせ体験
  07 Oct. 2023 - 07 Oct. 2023
  Organizing member
  北海道教育大学岩見沢校
  あそびプロジェクト vol.15
• 体験☆負けるな小腸・頑張れ免疫！
  07 Aug. 2023 - 07 Aug. 2023
  Advisor, Organizing member
  北海道大学理学部生物科学科 (高分子機能学)
  北海道大学オープンキャンパス2023・高校生限定プログラム
  北海道大学大学院 先端生命科学研究院 自然免疫研究室
  High school students
• 体験☆負けるな小腸・頑張れ免疫！
  08 Aug. 2022 - 08 Aug. 2022
  Advisor, Organizing member
  北海道大学理学部生物科学科 (高分子機能学)
  北海道大学オープンキャンパス2022・高校生限定プログラム
  北海道大学大学院 先端生命科学研究院 自然免疫研究室
  High school students
• 体験☆負けるな小腸・頑張れ免疫！
  05 Aug. 2019 - 05 Aug. 2019
  Advisor, Organizing member
  北海道大学理学部生物科学科 (高分子機能学)
  北海道大学オープンキャンパス2019・高校生限定プログラム
  北海道大学大学院 先端生命科学研究院 自然免疫研究室
  High school students
• Immunostaining
  31 Jul. 2019 - 31 Jul. 2019
  Advisor, Planner, Organizing member
  International School of Science
  International School of Science Japan 2018 SUMMER PROGRAM
  北海道大学大学院 先端生命科学研究院 自然免疫研究室
  Junior high school students, High school students
• 体験☆負けるな小腸・がんばれ免疫！
  07 Aug. 2018 - 07 Aug. 2018
  Advisor, Organizing member
  北海道大学理学部生物科学科 (高分子機能学)
  ひらめき☆ときめきサイエンス～ようこそ大学の研究室へ～KAKENHI
  北海道大学大学院 先端生命科学研究院 自然免疫研究室
  High school students
  小腸のパネト細胞 (Paneth cell)は、バイ菌がやってくるとαディフェンシンを分泌して自然免疫の作用ですばやく殺菌します。実験を通して、からだの中で働いている「免疫」を体験しましょう。
  ① 免疫の現場、小腸の組織を見てみよう！
  ② 免疫の瞬間、αディフェンシンが細菌を秒殺するのを目撃しよう！
  ③ 免疫のエース、パネト細胞の顆粒を光らせてみよう！小腸を色素で染色、位相差顕微鏡で観察し、さらに、パネト細胞の顆粒にあるαディフェンシンを蛍光免疫染色して、共焦点レーザー顕微鏡で詳しく観察しよう。
• 体験☆負けるな小腸・頑張れ免疫！
  06 Aug. 2018 - 06 Aug. 2018
  Advisor, Organizing member
  北海道大学理学部生物科学科 (高分子機能学)
  北海道大学オープンキャンパス2018・高校生限定プログラム
  北海道大学大学院 先端生命科学研究院 自然免疫研究室
  High school students
• 体験☆負けるな小腸・がんばれ免疫！
  08 Aug. 2017 - 08 Aug. 2017
  Advisor, Organizing member
  北海道大学理学部生物科学科 (高分子機能学)
  ひらめき☆ときめきサイエンス～ようこそ大学の研究室へ～KAKENHI
  北海道大学大学院 先端生命科学研究院 自然免疫研究室
  High school students
  小腸のパネト細胞 (Paneth cell)は、バイ菌がやってくるとαディフェンシンを分泌して自然免疫の作用ですばやく殺します。実験を通して「免疫」を体験しましょう。
  
  ① 免疫の現場、小腸の組織を見てみよう！
  ② 免疫の瞬間、αディフェンシンが細菌を秒殺するのを目撃しよう！
  ③ 免疫のエース、パネト細胞の顆粒を光らせてみよう！
  マウスの小腸を色素で染色、位相差顕微鏡で観察し、さらに、 パネト細胞の顆粒にあるαディフェンシンを蛍光免疫染色して、 共焦点レーザー顕微鏡で詳しく観察しよう。
• 体験☆負けるな小腸・頑張れ免疫！
  07 Aug. 2017 - 07 Aug. 2017
  Advisor, Organizing member
  北海道大学理学部生物科学科 (高分子機能学)
  北海道大学オープンキャンパス2017・高校生限定プログラム
  北海道大学大学院 先端生命科学研究院 自然免疫研究室
  High school students