Researcher basic information

• 博士(農学), 東京大学

• https://researchmap.jp/chromosomes?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201701012112293425

• 00631194

• 201701012112293425

• proteomics
• chromatin
• chromosome
• Senescence
• mitosis
• 染色体分配
• 細胞分裂

• Life Science, System genome science
• Life Science, Cell biology
• Life Science, Molecular biology

• Master's degree program, Graduate School of Life Science
• Doctoral (PhD) degree program, Graduate School of Life Science

Research activity information

• Dec. 2017, 高知信用金庫・高知安心友の会, 学術賞
  太田信哉
• Jul. 2017, 日本プロテオーム学会, 奨励賞
  太田信哉

• A role for condensin-mediator interaction in mitotic chromosome organization
  Osamu Iwasaki; Sanki Tashiro; Claire Yik-Lok Chung; Tomomi Hayashi; Hideki Tanizawa; Xuebing Wang; Shinya Ohta; Yuko Fujioka; Joseph Han; Gabrielle Tabor; Mikihiro Kawagoe; Ronen Marmorstein; Nobuo N. Noda; Ken-ichi Noma
  Nature Communications, Springer Science and Business Media LLC, 08 Feb. 2026
  Scientific journal
• DeepSAHF: deep learning-based detection of senescence-associated chromatin features reveals nuclear heterogeneity
  Claire Yik-Lok Chung; Shinya Ohta; HidekiTanizawa; Ken-ichi Noma
  Briefings in Bioinformatics, 26, Supplement_1, i27, i27, Oxford University Press (OUP), 12 Dec. 2025
  Scientific journal, Abstract
  
  Senescence-associated heterochromatin foci (SAHF) are condensed chromatin domains that mark cellular aging. Manual microscopy-based counting of SAHF-positive nuclei is widely used but is labor-intensive, subjective, and limited by binary classification that overlooks nuclear heterogeneity, especially in intermediate senescence or under non-canonical chromatin features. Accurate automated detection remains challenging due to variability in nuclear morphology, fluorescence intensity, and imaging conditions, with current approaches relying on arbitrary thresholds that hinder reproducibility.
  
  We present DeepSAHF, a deep learning–based pipeline for robust and reproducible detection of senescence-specific chromatin states from fluorescence microscopy images. The workflow integrates image pre-processing, YOLO-based single nucleus segmentation, and chromatin pattern classification to identify SAHF-positive cells. An extension using a visual language model (VLM) systematically comments on nuclear shape and features, facilitating interpretability and filtering unsuitable nuclei (e.g., out-of-focus). DeepSAHF is trained and tested at 20× resolution on oncogene-induced senescence cells across multiple time points, using curated high-confidence images from literature and novel data to ensure generalization without manual thresholds.
  
  By enabling scalable, reproducible, and interpretable analysis of chromatin reorganization, DeepSAHF advances beyond traditional methods, opening avenues to explore nuclear heterogeneity in aging and stress responses, with broad applications in drug screening, regenerative medicine, and cancer biology.
• Senotherapeutic potential against xeroderma pigmentosum
  Xuebing Wang; Masako Nishida; Ai Yoshioka; Claire Yik-Lok Chung; Shinya Hashimoto; Hideki Tanizawa; Shinya Ohta; Ken-ichi Noma; Takeshi Fukumoto
  bioRxiv : the preprint server for biology, Cold Spring Harbor Laboratory, 23 May 2025
  Abstract
  
  Xeroderma pigmentosum (XP) is an inherited photoaging syndrome caused by mutations in genes involved in the nucleotide excision repair (NER) pathway. XP patients exhibit hypersensitivity to ultraviolet (UV) radiation, leading to accelerated skin aging and requiring lifelong sun avoidance. Here, we demonstrate that UV-induced DNA damage triggers cellular senescence and up-regulates senescence-associated secretory phenotype (SASP) genes in melanocytes derived from an XP patient. To explore the potential therapeutics for XP, we developed a cisplatin-based drug screening system and identified JAK inhibitors and curcuminoids as promising senomorphic agents. In addition, two classes of senolytic agents, BCL-2-like protein inhibitors and HSP90 inhibitors, effectively eliminate senescent melanocytes. Further analysis demonstrates that senomorphic treatment effectively counteracts senescence and reduces SASP gene expression in XP-derived melanocytes. Moreover, genes in senescence-related pathways, including the JAK/STAT, Type I interferon (IFN-I), and PI3K/AKT pathways, which are activated by both UV irradiation and cisplatin treatment, are down-regulated by senomorphic treatment. This study highlights a potential senotherapeutic strategy for XP, which may help alleviate photoaging symptoms in XP patients.
• RNA methyltransferase SPOUT1/CENP-32 links mitotic spindle organization with the neurodevelopmental disorder SpADMiSS
  Dharmadhikari AV; Abad MA; Khan S.; Maroofian R.; Sands TT; Ullah F.; Samejima I.; Wear MA; Moore KE; Kondakova E.; Mitina N.; Schaub T.; Lee GK; Umandap CH; Berger SM; Iglesias AD; Popp B.; Jamra RA; Gabriel H.; Rentas S.; Rippert AL; Izumi K.; Conlin LK; Koboldt DC; Mosher TM; Hickey SE; Albert DVF; Norwood H.; Lewanda AF; Dai H.; Liu P.; Mitani T.; Marafi D.; Pehlivan D.; Posey JE; Lippa N.; Vena N.; Heinzen EL; Goldstein DB; Mignot C.; de Sainte; Agathe JM; Al-Sannaa NA; Zamani M.; Sadeghian S.; Azizimalamiri R.; Seifia T.; Zaki MS; Abdel-Salam GMH; Abdel-Hamid M.; Alabdi L.; Alkuraya FS; Dawoud H.; Lofty A.; Bauer P.; Zifarelli G.; Afzal E.; Zafar F.; Efthymiou S.; Gossett D.; Towne MC; Yeneabat R.; Wontakal SN; Aggarwal VS; Rosenfeld JA; Tarabykin V.; Ohta S.; Lupski JR; Houlden H.; Earnshaw WC; Davis EE; Jeyaprakash AA; Liao J.
  Nature Communications, 16, 1, 1703, Feb. 2025, [Peer-reviewed]
  English, Scientific journal
• Novel Role of Zinc-Finger Protein 518 in Heterochromatin Formation on alpha-Satellite DNA
  Shinya Ohta; Jun-ichirou Ohzeki; Nobuko Sato; Hideki Tanizawa; Claire Chung; Ken-ichi Noma; Hiroshi Masumoto
  Nucleic Acids Research, 53, 2, gkae1162, 04 Dec. 2024, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Chemo-Senolytic Therapeutic Potential against Angiosarcoma
  Xuebing Wang; Claire Yik-Lok Chung; Ai Yoshioka; Shinya Hashimoto; Haruki Jimbo; Hideki Tanizawa; Shinya Ohta; Takeshi Fukumoto; Ken-ichi Noma
  Journal of Investigative Dermatology, 144, 10, 2285, 2297.e13, Elsevier BV, Oct. 2024
  Scientific journal
• CCDC86 is a novel Ki-67-interacting protein important for cell division
  Konstantinos Stamatiou; Aldona Chmielewska; Shinya Ohta; William C Earnshaw; Paola Vagnarelli
  Journal of Cell Science, 136, 2, jcs260391, Jan. 2023, [Peer-reviewed]
  English, Scientific journal
• Quantitative proteomics of the mitotic chromosome scaffold reveals the association of BAZ1B with chromosomal axes
  Ohta S; Taniguchi T; Sato N; Hamada M; Taniguchi H; Rappsilber J
  Molecular and Cellular Proteomics, 18, 2, 169, 181, Feb. 2019, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry
  Jan G Ruppert; Kumiko Samejima; Melpomeni Platani; Oscar Molina; Hiroshi Kimura; A Arockia Jeyaprakash; Shinya Ohta; William C. Earnshaw
  EMBO Journal, 37, 6, e97677, Wiley-VCH Verlag, 15 Mar. 2018, [Peer-reviewed]
  English, Scientific journal
• Proteomics analysis understanding the regulation system of mitotic chromosome structure
  Shinya Ohta
  Proteome Letters, 2, 2, 59, 67, Jan. 2018, [Peer-reviewed], [Invited]
  Japanese, Scientific journal
• Nano Random Forests to mine protein complexes and their relationships in quantitative proteomics data
  Luis F. Montano-Gutierrez; Shinya Ohta; Georg Kustatscher; William C. Earnshaw; Juri Rappsilber
  MOLECULAR BIOLOGY OF THE CELL, 28, 5, 673, 680, Mar. 2017, [Peer-reviewed]
  English, Scientific journal
• 分裂期染色体におけるタンパク質修飾の時空間変化の網羅的解析
  太田信哉
  第一三共生命科学研究財団研究報告集, 32, 277, 292, 第一三共生命科学研究振興財団, Nov. 2016, [Invited]
  Japanese
• Identification of Mitosis-Specific Phosphorylation in Mitotic Chromosome-Associated Proteins
  Shinya Ohta; Michiko Kimura; Shunsuke Takagi; Iyo Toramoto; Yasushi Ishihama
  JOURNAL OF PROTEOME RESEARCH, 15, 9, 3331, 3341, Sep. 2016, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Proteomics Analysis with a Nano Random Forest Approach Reveals Novel Functional Interactions Regulated by SMC Complexes on Mitotic Chromosomes
  Shinya Ohta; Luis F. Montano-Gutierrez; Flavia de Lima Alves; Hiromi Ogawa; Iyo Toramoto; Nobuko Sato; Ciaran G. Morrison; Shunichi Takeda; Damien F. Hudson; Juri Rappsilber; William C. Earnshaw
  Molecular and Cellular Proteomics, 15, 8, 2802, 2818, Aug. 2016, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability
  Laura Wood; Daniel G. Booth; Giulia Vargiu; Shinya Ohta; Flavia Delima Alves; Kumiko Samejima; Tatsuo Fukagawa; Juri Rappsilber; William C. Earnshaw
  OPEN BIOLOGY, 6, 1, 150230, Jan. 2016, [Peer-reviewed]
  English, Scientific journal
• Polyglutamylated Tubulin Binding Protein C1orf96/CSAP Is Involved in Microtubule Stabilization in Mitotic Spindles
  Shinya Ohta; Mayako Hamada; Nobuko Sato; Iyo Toramoto
  PLOS ONE, 10, 11, e0142798, Nov. 2015, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly
  Shinya Ohta; Laura Wood; Iyo Toramoto; Ken-Ichi Yagyu; Tatsuo Fukagawa; William C. Earnshaw
  MOLECULAR BIOLOGY OF THE CELL, 26, 7, 1225, 1237, Apr. 2015, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• プロテオミクスと遺伝学による分裂期の理解
  太田信哉
  上原記念生命科学財団研究報告集, 28, 120, 4, 上原記念生命科学財団, Dec. 2014, [Invited]
  Japanese
• 多次元プロテオミクスを利用した分裂期を制御する癌原因遺伝子の探索とその機能の決定
  太田信哉
  かなえ医薬振興財団研究業績集, 41, Dec. 2014, [Invited]
  Japanese
• Phosphoproteomic analysis of mitotic chromatin
  Shinya Ohta
  The NOVARTIS Foundation (Japan) for the Promotion of Science, Annual Report, 26, 124, 126, Aug. 2014, [Invited]
  English
• Multi-classifier combinatorial proteomics proves useful for the analysis of the cellular structures that lack defined boundaries
  Shinya Ohta
  The lung, 21, 3, 294, 299, Aug. 2013, [Invited]
  Japanese, Scientific journal
• Mitotic chromosomes are compacted laterally by KIF4 and condensin and axially by topoisomerase II alpha
  Kumiko Samejima; Itaru Samejima; Paola Vagnarelli; Hiromi Ogawa; Giulia Vargiu; David A. Kelly; Flavia de Lima Alves; Alastair Kerr; Lydia C. Green; Damien F. Hudson; Shinya Ohta; Carol A. Cooke; Christine J. Farr; Juri Rappsilber; William C. Earnshaw
  JOURNAL OF CELL BIOLOGY, 199, 5, 755, 770, Nov. 2012, [Peer-reviewed]
  English, Scientific journal
• Building mitotic chromosomes
  Shinya Ohta; Laura Wood; Jimi-Carlo Bukowski-Wills; Juri Rappsilber; William C. Earnshaw
  CURRENT OPINION IN CELL BIOLOGY, 23, 1, 114, 121, Feb. 2011, [Peer-reviewed], [Lead author]
  English, Scientific journal
• The Protein Composition of Mitotic Chromosomes Determined Using Multiclassifier Combinatorial Proteomics
  Shinya Ohta; Jimi-Carlo Bukowski-Wills; Luis Sanchez-Pulido; Flavia de Lima Alves; Laura Wood; Zhuo A. Chen; Melpi Platani; Lutz Fischer; Damien F. Hudson; Chris P. Ponting; Tatsuo Fukagawa; William C. Earnshaw; Juri Rappsilber
  CELL, 142, 5, 810, 821, Sep. 2010, [Peer-reviewed], [Lead author]
  English, Scientific journal
• Proteomics of isolated mitotic chromosomes identifies the kinetochore protein Ska3/Rama1.
  Ohta S; Bukowski-Wills JC; Wood L; de Lima Alves F; Chen Z; Rappsilber J; Earnshaw WC
  Cold Spring Harbor symposia on quantitative biology, 75, 433, 438, 2010, [Peer-reviewed], [Lead author]
• Molecular and genetic analysis of condensin function in vertebrate cells
  Damien F. Hudson; Shinya Ohta; Tina Freisinger; Fiona MacIsaac; Lau Sennels; Flavia Alves; Fan Lai; Alastair Kerr; Juri Rappsilber; William C. Earnshaw
  MOLECULAR BIOLOGY OF THE CELL, 19, 7, 3070, 3079, Jul. 2008, [Peer-reviewed]
  English, Scientific journal
• Involvement of two domains with helix-turn-helix and zinc finger motifs in the binding of IS1 transposase to terminal inverted repeats
  S Ohta; E Yoshimura; E Ohtsubo
  MOLECULAR MICROBIOLOGY, 53, 1, 193, 202, Jul. 2004, [Peer-reviewed], [Lead author]
  English, Scientific journal
• A novel IS element, IS621, of the IS110/IS492 family transposes to a specific site in repetitive extragenic palindromic sequences in Escherichia coli
  S Choi; S Ohta; E Ohtsubo
  JOURNAL OF BACTERIOLOGY, 185, 16, 4891, 4900, Aug. 2003, [Peer-reviewed]
  English, Scientific journal
• Titanium alkoxide complex of functionalized conjugated dienes. A versatile bis-anionic template for stereoselective construction of carbon frameworks
  H Urabe; K Mitsui; S Ohta; F Sato
  JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 125, 20, 6074, 6075, May 2003, [Peer-reviewed]
  English, Scientific journal
• Presence of a characteristic D-D-E motif in IS1 transposase
  S Ohta; K Tsuchida; S Choi; Y Sekine; Y Shiga; E Ohtsubo
  JOURNAL OF BACTERIOLOGY, 184, 22, 6146, 6154, Nov. 2002, [Peer-reviewed], [Lead author]
  English, Scientific journal

• 分裂期染色体上におけるタンパク質リン酸化の網羅的解析
  太田信哉; 高木俊輔; 木村迪子; 石濱泰, 日本分子生物学会年会プログラム・要旨集(Web), 37th, 2P-0335 (WEB ONLY), 2014
  Japanese
• Defining the structure of mitotic chromosomes using multi-classifier combinatorial proteomics together with DT40 genetics
  Shinya Ohta; Laura C. Wood; Itaru Samejima; Flavia De Lima Alves; Juri Rapsilber; William C. Earnshaw, GENES & GENETIC SYSTEMS, 88, 6, 388, 388, Dec. 2013
  English, Summary international conference
• Understanding the architecture of vertebrate kinetochores using quantitative proteomics
  L. Wood; S. Ohta; K. Samejima; T. Fukagawa; J. Rappsilber; W. Earnshaw, MOLECULAR BIOLOGY OF THE CELL, 23, 2012
  English, Summary international conference
• The protein composition of mitotic chromosomes determined using multi-classifier combinatorial proteomics
  Shinya Ohta; Jimi-Carlo Bukowski-Wills; Luis Sanchez-Pulido; Flavia de Lima Alves; Laura Wood; Zhuo Chen; Melpi Platani; Lutz Fischer; Damien F. Hudson; Chris P. Ponting; Tatsuo Fukagawa; William C. Earnshaw; Juri Rappsilber, MUTAGENESIS, 25, 6, 635, 635, Nov. 2010
  English, Summary international conference

• 細胞老化に伴うクロマチンダイナミクスをプロテオミクスで追跡
  太田 信哉; 谷澤 英樹; 鍾 奕洛; 小迫 英尊; 野間 健一
  第98回日本生化学会大会, 05 Nov. 2025, Nominated symposium
  [Invited]
• プロテオミクスで追跡する細胞老化クロマチンダイナミクス
  太田 信哉
  第3回北海道バイオMix-up, 30 Sep. 2025, Oral presentation
  29 Sep. 2025 - 30 Sep. 2025
• Proteomics analysis of chromatin dynamics during cellular senescence
  Shinya Ohta; Hideki Tanizawa; Claire Yik-Lok Chung; Hidetaka Kosako; Ken-ichi Noma
  日本プロテオーム学会2025年大会, 07 Aug. 2025, Invited oral presentation
  [Invited]
• Proteomics analysis of chromatin dynamics during cellular senescence
  Shinya Ohta; Hideki Tanizawa; Claire Yik-Lok Chung; Hidetaka Kosako; Ken-ichi Noma
  第62回 日本生化学会北海道支部例会, 14 Jul. 2025, Oral presentation
• 細胞老化に伴う核内構造変化を制御するタンパク質を明らかにする
  太田 信哉; 谷澤 英樹; 鍾 奕洛; 小迫 英尊; 野間 健一
  第47回日本分子生物学会年会, Nov. 2024, Poster presentation
• 細胞老化における核内タンパク質動態の解読
  太田 信哉; 谷澤 英樹; 王 雪冰; 鍾 奕洛; 小迫 英尊; 野間 健一
  日本プロテオーム学会2024年大会・第20回日本臨床プロテオゲノミクス学会 合同大会, 24 Jun. 2024, Oral presentation
• Novel roles of zinc-finger protein 518s in the formation of human pericentromeric heterochromatin
  Shinya Ohta; Jun-Ichirou Ohzeki; Hideki Tanizawa; Claire Yik-Lok Chung; Hiroshi Masumoto; Ken-ichi Noma
  EMBO meeting, Chromatin dynamics and nuclear organization in genome maintenance, 21 Jun. 2024, English, Poster presentation
  20 Jun. 2024 - 23 Jun. 2024
• 細胞老化に伴う核内プロテオームの変化
  太田 信哉; 王 雪冰; 鍾 奕洛; 谷澤 英樹; 小迫 英尊; 野間 健一
  第21回日本プロテオーム学会2023年会, 19 Jul. 2023, Oral presentation
• Formation of pericentromeric heterochromatin via ZNF518s that link satellite DNA to heterochromatin
  太田 信哉; 野間 健一
  第17回生命医科学研究所ネットワーク国際シンポジウム, Oct. 2022, Poster presentation
• ZNF518を介したペリセントロメアへのヒストンメチル化酵素の誘導
  太田 信哉
  第94回日本遺伝学会年会, Sep. 2022, Oral presentation
• ZNF518によるCENP-B依存的なペリセントロメアヘテロクロマチン化メカニズム
  太田 信哉
  第93回日本遺伝学会年会, Sep. 2021, Oral presentation
• ZNF518によるCENP-B依存的なペリセントロメアヘテロクロマチン化メカニズム
  太田 信哉
  第38回染色体ワークショップ・第19回核ダイナミクス研究会、福岡(オンライン, Jan. 2021, Poster presentation
• ZNF518 proteins are involved in CENP-B dependent pericentromeric heterochromatization
  太田 信哉
  第43回日本分子生物学会年会, Dec. 2020, Poster presentation
• Proteomics analysis of mitotic chromosomes with using nano Random Forest
  Shinya Ohta
  The Korean Human Proteome Organization Annual International Proteomics Conference, Mar. 2019, English, Invited oral presentation
  Mar. 2019, [Invited]
• Proteomics analysis of mitotic chromosomes
  Shinya Ohta
  第15回日本プロテオーム学会2017年会, Jul. 2017, Japanese, Keynote oral presentation
  [Invited], [Domestic Conference]
• Quantitative proteomics revealed that BAZ1 proteins regulate the precious timing of chromosome condensation in early mitosis
  Shinya Ohta
  第15回日本プロテオーム学会2017年会, Jul. 2017, English, Invited oral presentation
  [Invited], [International presentation]
• Quantitative proteomics revealed that BAZ1 proteins regulate the precious timing of chromosome condensation in early mitosis
  Shinya Ohta
  SMC protein Workshop 2017, Jun. 2017, English, Poster presentation
  [International presentation]
• In silico co-fractionation を用いた分裂期染色体上のタンパク質相互作用解析
  太田信哉
  第13回日本プロテオーム学会2015年会 シンポジウム｢エピゲノム、プロテオゲノム｣, 21 Jul. 2015, Japanese, Invited oral presentation
  [Invited], [Domestic Conference]
• 多次元プロテオミクスを用いた分裂期染色体構造の解析
  太田信哉
  第2回JHUPOサテライトシンポジウム, 07 Jun. 2013, Japanese, Oral presentation
  [Invited], [Domestic Conference]
• Proteomics study of vertebrate mitotic chromosomes
  Itaru Samejima; Flavia de Lima Alves; Shinya Ohta; Juri Rappsilber; Tatsuo Fukagawa; William C Earnshaw
  Gordon Research Conference – chromosome dynamics, Jun. 2013, English, Poster presentation
  [International presentation]
• Defining the structure of mitotic chromosomes using multi-classifier combinatorial proteomics together with DT40 genetics
  Shinya Ohta; Laura Wood; Jimi-Carlo Bukowski-Wills; Itaru Samejima Luis Fernando Montano; Juri Rappsilber; William C. Earnshaw
  12th Human Proteome Organisation World Congress, Jun. 2013, English, Oral presentation
  [International presentation]
• Understanding the architecture of vertebrate kinetochores using quantitative proteomics
  Laura Wood; Shinya Ohta; Kumiko Samejima; Tatsuo Fukagawa; Juri Rappsilber; William C Earnshaw
  The American Society for Cell Biology Annual Meeting, Dec. 2012, English, Poster presentation
  [International presentation]
• Defining the complete protein composition of mitotic chromosomes with multi-classifier combinatorial proteomics
  Shinya Ohta; Jimi-Carlo Bukowski-Wills; Laura Wood; Melpi Platani; Flavia de Lima Alves; Juri Rappsilber; William C. Earnshaw
  The 6th UK-JAPAN cell cycle workshop, Mar. 2011, English, Poster presentation
  [International presentation]
• Understanding the architecture of vertebrate kinetochores using quantitative proteomics
  Laura Wood; Shinya Ohta; Jimi-Carlo Bukowski-Wills; Flavia de Lima Alves; Tatsuo Fukagawa; Juri Rappsilber; William C Earnshaw
  The 18th International Chromosome Conference, 2011, English, Poster presentation
  [International presentation]
• The protein composition of mitotic chromosomes determined using multi-classifier combinatorial proteomics
  Shinya Ohta; Jimi-Carlo Bukowski-Wills; Laura Wood; Juri Rappsilber; William C. Earnshaw
  The 75th Symposium: Nuclear Organization & Function 2010, Jul. 2010, English, Invited oral presentation
  [Invited], [International presentation]
• Using multi-dimensional proteomics to define the complete protein composition of mitotic chromosomes
  William C. Earnshaw; Shinya Ohta; Jimi-Carlo Bukowski-Wills; Flavia de Lima Alves; Lau Sennels; Juri Rappsilber
  34th Congress of the Federation-of-European-Biochemical-Societies, Jul. 2009, English, Invited oral presentation
  [Invited], [International presentation]
• Using multi-dimensional proteomics to define the complete protein composition of mitotic chromosomes
  Shinya Ohta; Flavia Alves; Lau Sennels; Juri Rappsilber; William C. Earnshaw
  The American Society for Cell Biology Annual Meeting, Dec. 2008, English, Poster presentation
  [International presentation]
• ATPase function and binding of condenin in vertebrate cells
  Damien F. Hudson; Shinya Ohta; William C. Earnshaw
  The American Society for Cell Biology Annual Meeting, Dec. 2008, English, Poster presentation
  [International presentation]
• Defining the complete protein composition of mitotic chromosomes with multidimensional proteomics
  Shinya Ohta; Flavia Alves; Damien F. Hudson; Paola Vagnarelli; Lau Sennels; Juri Rappsilber; William C. Earnshaw
  EMBO workshop Chromosome segregation: Centromere & Kinetochores, Nov. 2008, English, Poster presentation
  [International presentation]
• The structure-specific DNA binding activity in IS1 transposase
  Shinya Ohta; Eiichi Ohtsubo
  ASM conference, Mobile DNA, Feb. 2006, English, Poster presentation
  [International presentation]
• Involvement of two domains with Helix-Turn-Helix and Zinc Finger motif in the binding of IS1 transposase to terminal inverted repeats.
  Shinya Ohta; Etsuro Yoshimura; Eiichi Ohtsubo
  Keystone Symposia, Bacteria chromosome, 2004, English, Poster presentation
  [International presentation]
• Involvement of two domains with helix-turn-helix and zincf motif in the binding of IS1 transposase to terminal inverted repeats.
  Shinya Ohta; Etsuro Yoshimura; Eiichi Ohtsubo
  The 4th International Symposium on DNA Replication, Recombination and Repair, 2003, English, Oral presentation
  [International presentation]

• 生物学Ⅰ, 2024年, 学士課程, 全学教育

• 3Dゲノム構造を介した細胞老化に伴う遺伝子発現変容機構
  科学研究費助成事業
  01 Apr. 2025 - 31 Mar. 2029
  太田 信哉; 谷澤 英樹
  日本学術振興会, 基盤研究(C), 北海道大学, 25K14883
• 染色体分離と転写を結び付ける分子機構の解明
  科学研究費助成事業
  01 Apr. 2025 - 31 Mar. 2028
  野間 健一; 太田 信哉; 谷澤 英樹
  日本学術振興会, 基盤研究(B), 北海道大学, 25K02249
• 老化細胞ゲノム構造制御を基盤とした小児性早老疾患の病態理解と介入可能性の探索
  バイオ分野 研究開発助成
  Apr. 2026 - Mar. 2027
  太田 信哉
  G-7奨学財団, Principal investigator
• 老化が病気に変わる瞬間を捉える：3D ゲノムで病の引き金を探る
  研究助成金
  Apr. 2026 - Mar. 2027
  太田 信哉
  寿原記念財団, Principal investigator
• 分裂酵母とヒトの３Ｄゲノム構造とその形成機構の解明
  科学研究費助成事業
  Feb. 2022 - Mar. 2025
  野間 健一; 太田 信哉
  日本学術振興会, 国際共同研究加速基金(帰国発展研究), 北海道大学, 20K23376
• 機能未知タンパク質ZNF518を介したペリセントロメアの新規ヘテロクロマチン形成機構
  国際研究集会出張助成
  Jun. 2024 - Jul. 2024
  太田 信哉
  小笠原敏晶記念財団, Principal investigator
• 老化細胞が老化因子を分泌する仕組みをゲノムの３次元構造変化で解明する
  研究助成
  Sep. 2023 - Mar. 2024
  太田 信哉
  秋山記念生命科学振興財団, Principal investigator
• タンパク質リン酸化制御ネットワークの網羅的予測技術の開発
  2019年度 研究助成
  Apr. 2020 - Mar. 2022
  太田 信哉
  中部電気利用基礎研究振興財団, Principal investigator
• Novel mechanism of pericentromere-specific heterochromatin formation
  Grants-in-Aid for Scientific Research
  Apr. 2018 - Mar. 2021
  Ohta Shinya
  Centromere/kinetochore is essential for proper segregation of the genome during mitosis. It has been indicated that precise heterochromatization at pricentromeric alpha-satellites is required for maintenance of centromere function. Currently our proteomics has indicated chromosomal association of uncharacterized protein, ZNF518. Therefore we verified this hypothesis observing localization of GFP fusion and found its pericentromere localization with satellite DNA binding protein CENP-B dependent manner. Also GFP localization trucking with various truncated ZNF518 shows two essential domains for the pericentromere localization. A fluorescence microscopy-based interaction-trap assay on human chromosomes suggests these two domains are independent CENP-B or HP1 interacting domains. Since G9a histone metyltransferase interaction of ZNF518 has been reported, we suppose that ZNF518 protein is involved in CENP-B dependent pericentromeric heterochromatization.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), 18K06061
• セントロメア周縁部のヘテロクロマチン形成と維持の機構に関与する未知タンパク質
  研究助成
  Apr. 2016 - Mar. 2018
  太田 信哉
  加藤記念バイオサイエンス振興財団, Principal investigator
• ヘテロクロマチンの形成と維持の機構を担う機能未知なセントロメア結合タンパク質
  医学系研究奨励
  Apr. 2016 - Mar. 2018
  太田 信哉
  武田科学振興財団, Principal investigator
• Functional analysis of novel centromere/kinetochore proteins in mitosis
  Grants-in-Aid for Scientific Research
  Apr. 2016 - Mar. 2018
  Ohta Shinya
  Our proteomics analysis of mitotic chromosomes revealed that uncharacterized protein MKT4 localizes to centromere in mitosis. Functional analysis suggested the involvement of MKT4 in the mitotic spindle checkpoint depending on microtubules. Moreover MKT4 shows most strong kinetochore localization in prophase and disassociation by anaphase onset. In this study, we found another novel centromere protein ZNF518B which associates centromere through the cell cycle. ZNF518B and its homolog ZNF518A interact Heterochromatin protein 1 (HP1) and Centromere protein B (CENP-B), which associate pericentromere region in chromosomes.
  Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Kochi University, 16K18494
• 癌化を抑制する新規染色体タンパク質の機能解析
  研究助成金
  Dec. 2015 - Mar. 2017
  太田 信哉
  かなえ医薬振興財団, Principal investigator
• 分裂期染色体タンパク質における翻訳後修飾の網羅的解析
  基礎科学研究助成
  Nov. 2015 - Mar. 2017
  太田 信哉
  住友財団, Principal investigator
• プロテオミクスを基盤とした分裂期の理解と染色体分配制御に関わる新しい機構の発見
  日本人若手研究者研究助成
  Oct. 2013 - Mar. 2016
  太田 信哉
  中島記念国際交流財団, Principal investigator
• 染色体タンパク質の翻訳後修飾の時間的空間的変化と分裂期染色体動態の制御機構との関わりを解明する
  研究助成金
  Oct. 2013 - Mar. 2016
  太田 信哉
  持田記念医学薬学振興財団, Principal investigator
• 分裂期染色体におけるタンパク質修飾の時空間変化の網羅的解析
  創立30周年記念事業海外帰国研究者研究継続支援助成
  Oct. 2013 - Mar. 2016
  太田 信哉
  第一三共生命科学研究振興財団, Principal investigator
• Identification and functional analysis of novel vertebrate chromosomal proteins with using Multi-Classifier Combinational Proteomics
  Grants-in-Aid for Scientific Research
  Apr. 2013 - Mar. 2016
  Ohta Shinya
  In this study we identified CENP-32. CENP-32 appears to be required for centrosomes to integrate into a fully functional spindle that notonly nucleates astral microtubules, but also is able to nucleate and bind to kinetochore andcentral spindle microtubules. Additional data suggest that NuMA tethers microtubules at the anastral spindle poles and that augmin is required for centrosome detachment after CENP-32 depletion, possibly due to an imbalance of forces within the spindle.
  Next we identified CSAP and proposed that CSAP associates with MTs around centrosomes to stabilize MTs during mitosis, ensuring proper bipolar spindle formation and maintenance.
  Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Kochi University, 25870487
• 分裂期染色体におけるサブドメイン特異的タンパク質翻訳後修飾のプロテオミクス解析
  ノバルティス研究奨励金
  Apr. 2014 - Mar. 2015
  太田 信哉
  ノバルティス科学振興財団, Principal investigator
• プロテオミクスと遺伝学による分裂期の理解
  研究奨励金
  Jan. 2013 - Mar. 2015
  太田 信哉
  上原記念生命科学財団, Principal investigator
• 新規セントロメアタンパク質CENP-34, -36の機能とその欠失を介した染色体異常のメカニズムの解析
  医学系研究奨励
  Oct. 2012 - Mar. 2015
  太田 信哉
  武田科学振興財団, Principal investigator
• 多次元プロテオミクスを利用した分裂期を制御する癌原因遺伝子の探索とその機能の決定
  研究助成金
  Dec. 2012 - Mar. 2014
  太田 信哉
  かなえ医薬振興財団, Principal investigator
• 多次元プロテオミクスを利用した新規染色体タンパク質の探索
  科学研究費助成事業
  Aug. 2012 - Mar. 2014
  太田 信哉
  本研究の目的は、分裂期染色体の凝縮や分配機構に関わる新規のタンパク質を同定することである。この目的を達成するために、近年普及した定量的なプロテオーム解析をさらに改良し、複数の定量値を統計学的に組み合わせた手法、多次元プロテオミクスにおいて、分裂期染色体タンパク質と予測された機能未知タンパク質の分裂期細胞内での局在の決定を試み、これまでにこの手法で、12種類の新規セントロメアタンパク質を含む、30種類の新規染色体タンパク質を同定した。
  1 そこで本年度の研究では、更なる機能的分裂期染色体タンパク質同定のため、上記のプロテオミクス解析から染色体タンパク質と予測されるタンパク質の局在をさらに確認し、興味深いタンパク質を見いだすことを試みた。これまでに、100種類の標的遺伝子のうち25種類のヒトホモログをクローニングした。クローニングした遺伝子はGFP融合タンパク質の形でヒト細胞に導入したが、これまでに有力な新規遺伝子の発見には至っていない。
  2 また、同時に我々がこれまでに見いだした興味深い新規セントロメアタンパク質の機能解析を行った。これにより、この新規タンパク質は分裂前中期において染色体パッセンジャー複合体のセントロメアの局在と姉妹染色体の接着維持に必須であることが示唆された。
  日本学術振興会, 研究活動スタート支援, 高知大学, 24870020
• 挿入因子IS1がコードするトランスポゼースの構造と機能に関する研究
  科学研究費助成事業
  2005 - 2006
  太田 信哉
  トランスポゾンの転移は、トランスポゼースが両IRに結合した後、トランスポゼースの2量体形成により、両IRが近接した形の安定な複合体であるトランスポソゾームを形成することによって起こると考えられている。このような複合体においてトランスポゼースは、IR特異的DNA結合活性に加え、転移中間体中の少なくとも4本のDNA鎖が交叉した、ホリデー様構造に特異的なDNA結合活性を持つことにより、安定な複合体であるトランスポソゾームを形成しているのではないかと考えた。そこで、IRを含まないホリデー構造をとるX字形の基質を作成し、これを用いてトランスポゼースが結合するかどうか調べた。その結果、トランスポゼースはX字型DNAに結合し、2つのトランスポゼース分子が結合した複合体を形成することを見出した。また、トランスポゼースはX字型のみならず、Y字型DNAにも結合すること、この場合には1つのトランスポゼース分子が結合した複合体を形成すること、さらに、IR配列を持たない2本鎖DNAには結合しないが、バルジ構造を持つ2本鎖DNAには結合することが分かった。
  これらの結果は、トランスポゼースが折れ曲がった2本のDNAを認識するという構造特異的DNA結合活性を保持していることを示唆する。このことは、トランスポゼースがIR特異的DNA結合活性に加え、構造特異的DNA結合活性を保持することにより、安定な複合体であるトランスポソゾームを形成するという上記の仮説を支持する。
  次に、トランスポゼースのどの領域が上記の活性に関わっているか、いくつかのトランスポゼース欠失変異体を用いてX字型あるいはY字型DNAへの結合を調べた。その結果、トランスポゼースのN及びC末領域が、両基質への結合に関わっていることが分かった。また、N末領域においてはジンクフィンガーモチーフ、および、ジンクフィンガーとヘリックスーターンーヘリックスモチーフの問に存在するヘリックス領域が、C末領域においてはDDEモチーフの存在する領域が、この活性に関わることが分かった。
  また、トランスポゼースの結合ドメインの構造を解析するために、トランスポゼースのN末端領域とタンパク質Gの一部を融合したタンパク質を発現、生成した。これをサンプルとし、NMRを用いた構造解析を試みた。その結果、このタンパク質の一部のアミノ酸をNMRで認識することに成功した。
  日本学術振興会, 特別研究員奨励費, 東京大学, 05J11501