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SATO Takeo
| Faculty of Science Biological Sciences Cell Structure and Function | Associate Professor |
| Institute for Academic Innovation | Associate Professor |
Researcher basic information
■ Degree■ URL
researchmap URLホームページURL■ Various IDs
J-Global ID■ Research Keywords and Fields
Research Field■ Educational Organization
- Bachelor's degree program, School of Science
- Master's degree program, Graduate School of Life Science
- Doctoral (PhD) degree program, Graduate School of Life Science
Research activity information
■ Awards■ Papers
- Cellular energy sensor SnRK1 suppresses salicylic acid-dependent and -independent defenses and bacterial resistance in Arabidopsis
Linnan Jie; Miho Sanagi; Shigetaka Yasuda; Kohji Yamada; Saki Ejima; Ayumi Sugisaki; Junpei Takagi; Mika Nomoto; Xiufang Xin; Yasuomi Tada; Yusuke Saijo; Takeo Sato
Cold Spring Harbor Laboratory, 01 Oct. 2025, [Peer-reviewed], [Last author, Corresponding author]
In nature, plants cope with various pathogens that compete for cellular resources during infection. It has long been suggested that plant defense activity must be linked to cellular energy and metabolic states to optimize the balance between growth and defense. However, the molecular mechanisms that regulate immune activity in relation to cellular energy status remain unclear. Here, we demonstrate that the plant energy sensor SNF1-RELATED KINASE 1 (SnRK1) plays a critical role in modulating defense responses and bacterial resistance in Arabidopsis thaliana. Bacterial elicitor-induced expression of defense marker genes, such as PATHOGENESIS-RELATED 1 (PR1), is significantly repressed under sugar-limited conditions in wild-type seedlings, whereas this expression is markedly enhanced in the snrk1 knockdown mutants. SnRK1 restricts defense-related gene expression and resistance to the biotrophic bacterial pathogen Pseudomonas syringe pv. tomato DC3000, which are partly dependent on salicylic acid (SA). In addition, we found that the SnRK1 kinase activity is increased by high humidity. Consistently, SnRK1 is critical for the suppression of SA-mediated defense responses under high humidity conditions. SnRK1 physically associates with the SA-related transcription factors TGACG SEQUENCE-SPECIFIC BINDING PROTEIN 4 (TGA4) and TGA2 to attenuate PR1 expression. These findings provide valuable insight into the molecular mechanisms linking cellular energy status with immune regulation in plants. - New insights into nutrient- and drought-responsive flowering.
Miho Sanagi; Filip Rolland; Takeo Sato
Journal of experimental botany, 28 Mar. 2025, [Peer-reviewed], [Last author, Corresponding author], ［Internationally co-authored］, [International Magazine]
English, Scientific journal, Nutrients not only provide energy and structural components but also play essential roles as regulatory molecules to control plant growth and development. Flowering is a key developmental phase transition (from vegetative to reproductive growth), and its precise timing determines reproductive fitness and crop yield. This requires coordination of metabolism, partitioning between source and sink tissues, and apical meristem activity with nutrient supplies. Here we summarize recent advances in our understanding of nutrient-regulated flowering, focusing on sugars and the three primary (soil supplied) macronutrients nitrogen, phosphorus, and potassium, also considering drought stress as a highly relevant condition affecting nutrient availability. Most notably, recent evidence indicates that the evolutionarily conserved SNF1-RELATED KINASE 1 (SnRK1) kinase, a key metabolic sensor, serves as an integrator of nutrient status to control flowering. However, the combined effects of multiple nutrients on flowering and differences in responses between plant species remain underexplored and are an important topic for future research. - Citrate pretreatment promotes rice (Oryza sativa L.) coleoptile elongation under submergence
Akio Kubo; Miho Sanagi; Yuko Maki; Ryosuke Koyari; Futoshi Sakuma; Junji Yamaguchi; Takeo Sato
42, 57, 64, Mar. 2025, [Peer-reviewed], [Last author, Corresponding author], [International Magazine] - Recent Advances in Ubiquitin Signals Regulating Plant Membrane Trafficking.
Yoko Hasegawa; Yongming Luo; Takeo Sato
Plant & cell physiology, 65, 12, 1907, 1924, 21 Dec. 2024, [Peer-reviewed], [Last author, Corresponding author], ［Internationally co-authored］, [International Magazine]
English, Scientific journal, Ubiquitination is a reversible post-translational modification involving the attachment of ubiquitin, a 76-amino acid protein conserved among eukaryotes. The protein 'ubiquitin' was named after it was found to be ubiquitously expressed in cells. Ubiquitination was first identified as a post-translational modification that mediates energy-consuming protein degradation by the proteasome. After half a century, the manifold functions of ubiquitin are widely recognized to play key roles in diverse molecular pathways and physiological processes. Compared to humans, the number of enzymes related to ubiquitination is almost twice as high in plant species, such as Arabidopsis and rice, suggesting that this modification plays a critical role in many aspects of plant physiology including development and environmental stress responses. Here, we summarize and discuss recent knowledge of ubiquitination focusing on the regulation of membrane trafficking in plants. Ubiquitination of plasma membrane-localized proteins often leads to endocytosis and vacuolar targeting. In addition to cargo proteins, ubiquitination of membrane trafficking regulators regulates the morphodynamics of the endomembrane system. Thus, throughout this review, we focus on the physiological responses regulated by ubiquitination and their underlying mechanisms to clarify what is already known and what would be interesting to investigate in the future. - Plasmodesmata mediate cell-to-cell transport of brassinosteroid hormones
Yaowei Wang; Jessica Perez-Sancho; Matthieu Pierre Platre; Brenda Callebaut; Marija Smokvarska; Karoll Ferrer; Yongming Luo; Trevor M. Nolan; Takeo Sato; Wolfgang Busch; Philip N. Benfey; Miroslav Kvasnica; Johan M. Winne; Emmanuelle M. Bayer; Nemanja Vukašinović; Eugenia Russinova
Nature Chemical Biology, 19, 11, 1331, 1341, Springer Science and Business Media LLC, 26 Jun. 2023, [Peer-reviewed], ［Internationally co-authored］, [International Magazine]
Scientific journal - Histone chaperone NUCLEOSOME ASSEMBLY PROTEIN 1 proteins affect plant growth under nitrogen deficient conditions in Arabidopsis thaliana
Linnan Jie; Miho Sanagi; Yongming Luo; Haruna Maeda; Yoichiro Fukao; Yukako Chiba; Shuichi Yanagisawa; Junji Yamaguchi; Junpei Takagi; Takeo Sato
PLANT BIOTECHNOLOGY, 40, 1, 93, 98, Mar. 2023, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal - Flowering regulation in response to nitrogen availability
Sanagi Miho; Sato Takeo
生化学, 94, 6, 892, 895, 公益社団法人日本生化学会, 25 Dec. 2022, [Domestic magazines]
Japanese - Deubiquitinating enzymes UBP12 and UBP13 regulate carbon/nitrogen-nutrient stress responses by interacting with the membrane-localized ubiquitin ligase ATL31 in Arabidopsis
Yongming Luo; Shigetaka Yasuda; Junpei Takagi; Yoko Hasegawa; Yukako Chiba; Junji Yamaguchi; Takeo Sato
Biochemical and Biophysical Research Communications, Elsevier BV, Oct. 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
Scientific journal - Conjugates of 3-phenyllactic acid and tryptophan enhance root-promoting activity without adverse effects in Vigna angularis.
Yuko Maki; Hiroshi Soejima; Tamizi Sugiyama; Takeo Sato; Junji Yamaguchi; Masaaki K Watahiki
Plant Biotechnology, 39, 2, 173, 177, 25 Jun. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, 3-Phenyllactic acid (PLA) is a common secondary product of Lactobacillus sp. and promotes adventitious-root formation in Azuki beans (Vigna angularis). Root promotion activity of PLA is synergistically enhanced by tryptophan (Trp). In this study, stereoisomers of PLA and Trp amide conjugates and their alkyl esters were synthesized to investigate the structure-activity relationships on root-promotion activity. The rooting activity of D-PLA-L-Trp conjugate shows more than 40 times higher than that of the mixture of D-PLA and L-Trp. Modification of PLA-Trp with ethyl ester showed the highest activity at 3,400 times of a mixture of D-PLA and L-Trp. However, L-or D-PLA-D-Trp conjugate and the isopropyl ester of PLA-Trp conjugates, both lost the root promotion activity and implicated that a requirement for steric structure for PLA related root promotion mechanism. Unlike auxin substances, which are commonly used as rooting agents that displayed high activity in low concentrations, PLA-Trp ethyl ester exhibited far less phytotoxicity at high concentration of 1 mM, despite its high rooting activity. Innovation of PLA-Trp ethyl ester may be expected for agricultural aspects with low environmental impact. - 3-Phenyllactic acid is converted to phenylacetic acid and induces auxin-responsive root growth in Arabidopsis plants.
Yuko Maki; Hiroshi Soejima; Tamizi Sugiyama; Masaaki K Watahiki; Takeo Sato; Junji Yamaguchi
Plant biotechnology (Tokyo, Japan), 39, 2, 111, 117, 25 Jun. 2022, [Peer-reviewed], [International Magazine]
English, Scientific journal, Many microorganisms have been reported to produce compounds that promote plant growth and are thought to be involved in the establishment and maintenance of symbiotic relationships. 3-Phenyllactic acid (PLA) produced by lactic acid bacteria was previously shown to promote root growth in adzuki cuttings. However, the mode of action of PLA as a root-promoting substance had not been clarified. The present study therefore investigated the relationship between PLA and auxin. PLA was found to inhibit primary root elongation and to increase lateral root density in wild-type Arabidopsis, but not in an auxin signaling mutant. In addition, PLA induced IAA19 promoter fused β-glucuronidase gene expression, suggesting that PLA exhibits auxin-like activity. The inability of PLA to promote degradation of Auxin/Indole-3-Acetic Acid protein in a yeast heterologous reconstitution system indicated that PLA may not a ligand of auxin receptor. Using of a synthetic PLA labeled with stable isotope showed that exogenously applied PLA was converted to phenylacetic acid (PAA), an endogenous auxin, in both adzuki and Arabidopsis. Taken together, these results suggest that exogenous PLA promotes auxin signaling by conversion to PAA, thereby regulating root growth in plants. - Deubiquitinating enzymes UBP12 and UBP13 stabilize the brassinosteroid receptor BRI1.
Yongming Luo; Junpei Takagi; Lucas Alves Neubus Claus; Chao Zhang; Shigetaka Yasuda; Yoko Hasegawa; Junji Yamaguchi; Libo Shan; Eugenia Russinova; Takeo Sato
EMBO reports, 23, 4, e53354, 15 Feb. 2022, [Peer-reviewed], [Corresponding author], ［Internationally co-authored］, [International Magazine]
English, Scientific journal, Protein ubiquitination is a dynamic and reversible post-translational modification that controls diverse cellular processes in eukaryotes. Ubiquitin-dependent internalization, recycling, and degradation are important mechanisms that regulate the activity and the abundance of plasma membrane (PM)-localized proteins. In plants, although several ubiquitin ligases are implicated in these processes, no deubiquitinating enzymes (DUBs), have been identified that directly remove ubiquitin from membrane proteins and limit their vacuolar degradation. Here, we discover two DUB proteins, UBP12 and UBP13, that directly target the PM-localized brassinosteroid (BR) receptor BR INSENSITIVE1 (BRI1) in Arabidopsis. BRI1 protein abundance is decreased in the ubp12i/ubp13 double mutant that displayed severe growth defects and reduced sensitivity to BRs. UBP13 directly interacts with and effectively removes K63-linked polyubiquitin chains from BRI1, thereby negatively modulating its vacuolar targeting and degradation. Our study reveals that UBP12 and UBP13 play crucial roles in governing BRI1 abundance and BR signaling activity to regulate plant growth. - The TGN/EE SNARE protein SYP61 and the ubiquitin ligase ATL31 cooperatively regulate plant responses to carbon/nitrogen conditions in Arabidopsis.
Yoko Hasegawa; Thais Huarancca Reyes; Tomohiro Uemura; Anirban Baral; Akari Fujimaki; Yongming Luo; Yoshie Morita; Yasushi Saeki; Shugo Maekawa; Shigetaka Yasuda; Koki Mukuta; Yoichiro Fukao; Keiji Tanaka; Akihiko Nakano; Junpei Takagi; Rishikesh P Bhalerao; Junji Yamaguchi; Takeo Sato
The Plant cell, 34, 4, 1354, 1374, 28 Jan. 2022, [Peer-reviewed], [Corresponding author], ［Internationally co-authored］, [International Magazine]
English, Scientific journal, Ubiquitination is a post-translational modification involving the reversible attachment of the small protein ubiquitin to a target protein. Ubiquitination is involved in numerous cellular processes, including the membrane trafficking of cargo proteins. However, the ubiquitination of the trafficking machinery components and their involvement in environmental responses are not well understood. Here, we report that the Arabidopsis thaliana trans-Golgi network/early endosome (TGN/EE) localized SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) protein SYP61 interacts with the transmembrane ubiquitin ligase ATL31, a key regulator of resistance to disrupted carbon (C)/nitrogen/(N)-nutrient conditions. SYP61 is a key component of membrane trafficking in Arabidopsis. The subcellular localization of ATL31 was disrupted in knockdown mutants of SYP61, and the insensitivity of ATL31-overexpressing plants to high C/low N-stress was repressed in these mutants, suggesting that SYP61 and ATL31 cooperatively function in plant responses to nutrient stress. SYP61 is ubiquitinated in plants, and its ubiquitination level is upregulated under low C/high N-nutrient conditions. These findings provide important insights into the ubiquitin signaling and membrane trafficking machinery in plants. - Low nitrogen conditions accelerate flowering by modulating the phosphorylation state of FLOWERING BHLH 4 in Arabidopsis.
Miho Sanagi; Shoki Aoyama; Akio Kubo; Yu Lu; Yasutake Sato; Shogo Ito; Mitsutomo Abe; Nobutaka Mitsuda; Masaru Ohme-Takagi; Takatoshi Kiba; Hirofumi Nakagami; Filip Rolland; Junji Yamaguchi; Takato Imaizumi; Takeo Sato
Proceedings of the National Academy of Sciences of the United States of America, 118, 19, 11 May 2021, [Peer-reviewed], [Last author, Corresponding author], ［Internationally co-authored］, [International Magazine]
English, Scientific journal, Nitrogen (N) is an essential nutrient that affects multiple plant developmental processes, including flowering. As flowering requires resources to develop sink tissues for reproduction, nutrient availability is tightly linked to this process. Low N levels accelerate floral transition; however, the molecular mechanisms underlying this response are not well understood. Here, we identify the FLOWERING BHLH 4 (FBH4) transcription factor as a key regulator of N-responsive flowering in Arabidopsis Low N-induced early flowering is compromised in fbh quadruple mutants. We found that FBH4 is a highly phosphorylated protein and that FBH4 phosphorylation levels decrease under low N conditions. In addition, decreased phosphorylation promotes FBH4 nuclear localization and transcriptional activation of the direct target CONSTANS (CO) and downstream florigen FLOWERING LOCUS T (FT) genes. Moreover, we demonstrate that the evolutionarily conserved cellular fuel sensor SNF1-RELATED KINASE 1 (SnRK1), whose kinase activity is down-regulated under low N conditions, directly phosphorylates FBH4. SnRK1 negatively regulates CO and FT transcript levels under high N conditions. Together, these results reveal a mechanism by which N levels may fine-tune FBH4 nuclear localization by adjusting the phosphorylation state to modulate flowering time. In addition to its role in flowering regulation, we also showed that FBH4 was involved in low N-induced up-regulation of nutrient recycling and remobilization-related gene expression. Thus, our findings provide insight into N-responsive growth phase transitions and optimization of plant fitness under nutrient-limited conditions. - 3-Phenyllactic acid, a root-promoting substance isolated from Bokashi fertilizer, exhibits synergistic effects with tryptophan
Yuko Maki; Hiroshi Soejima; Toru Kitamura; Tamizi Sugiyama; Takeo Sato; Masaaki K. Watahiki; Junji Yamaguchi
Plant Biotechnology, 38, 1, 9, 16, Japanese Society for Plant Cell and Molecular Biology, 25 Mar. 2021, [Peer-reviewed], [International Magazine]
Scientific journal - Proteome Analysis of 14-3-3 Targets in Tomato Fruit Tissues.
Yongming Luo; Yu Lu; Junji Yamaguchi; Takeo Sato
Methods in molecular biology (Clifton, N.J.), 2139, 289, 296, 2020, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Tomato is a major crop plant and an important constituent of the human diet. Exclusive features such as bearing fleshy fruits and undergoing a phase transition from partially photosynthetic to fully heterotrophic metabolism make tomato fruit a model system for fruit development studies. Although the tomato genome has been completely sequenced, functional proteomics studies are still at their starting stage. Proteomics technologies, especially the combination of multiple approaches, provide a very powerful tool to accurately identify functional proteins and investigate certain sets of proteins in more detail. The direct binding of plant 14-3-3 proteins to their multiple target proteins modulates the functions of the latter, suggesting that these 14-3-3 proteins are directly involved in various physiological pathways. This chapter outline methods for the identification of 14-3-3 protein complexes in tomato fruit tissues. These methods include detailed protocols for protein extraction, coimmunoprecipitation, SDS-PAGE, SYPRO Ruby staining, in-gel trypsin digestion, and LC-MS/MS analysis for 14-3-3 interactomics. - Protein Phosphorylation Dynamics Under Carbon/Nitrogen-Nutrient Stress and Identification of a Cell Death-Related Receptor-Like Kinase in Arabidopsis.
Xingwen Li; Miho Sanagi; Yu Lu; Yuko Nomura; Sara Christina Stolze; Shigetaka Yasuda; Yusuke Saijo; Waltraud X Schulze; Regina Feil; Mark Stitt; John E Lunn; Hirofumi Nakagami; Takeo Sato; Junji Yamaguchi
Frontiers in plant science, 11, 377, 377, 2020, [Peer-reviewed], [Last author, Corresponding author], ［Internationally co-authored］, [International Magazine]
English, Scientific journal, Nutrient availability, in particular the availability of sugar [carbon (C)] and nitrogen (N), is important for the regulation of plant metabolism and development. In addition to independent utilization of C and N nutrients, plants sense and respond to the balance of C and N nutrients (C/N-nutrient) available to them. High C/low N-nutrient stress has been shown to arrest early post-germinative growth while promoting progression to senescence in Arabidopsis. Although several signaling components of the C/N-nutrient response have been identified, the inclusive molecular basis of plant C/N-nutrient response remains unclear. This proteome analysis evaluated phosphorylation dynamics in response to high C/low N-nutrient stress. Phosphoproteomics under conditions of C/N-nutrient stress showed a global change in the phosphorylation status of proteins, including plasma membrane H+-ATPase, carbon and nitrogen metabolic enzymes and signaling proteins such as protein kinases and transcription factors. Further analyses suggested that SNF1-related protein kinase 1 (SnRK1) is involved in primary C/N-nutrient signal mediation via the transcriptional regulation of C/N-regulatory kinases. We also identified a leucine-rich repeat receptor-like kinase with extracellular malectin-like domain, named as LMK1, which was shown to possess cell death induction activity in plant leaves. These results provide important insight into the C/N-nutrient signaling pathways connecting nutrition stress to various cellular and physiological processes in plants. - Identification of Arabidopsis CCR4-NOT Complexes with Pumilio RNA-Binding Proteins, APUM5 and APUM2.
Toshihiro Arae; Kotone Morita; Riko Imahori; Yuya Suzuki; Shigetaka Yasuda; Takeo Sato; Junji Yamaguchi; Yukako Chiba
Plant & cell physiology, 60, 9, 2015, 2025, 01 Sep. 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal, CCR4/CAF1 are widely conserved deadenylases in eukaryotes. They form a large complex that includes NOT1 as a scaffold protein and various NOT proteins that are core components of multiple levels of gene expression control. The CCR4-NOT complex also contains several RNA-binding proteins as accessory proteins, which are required for target recognition by CCR4/CAF1 deadenylases. AtCCR4a/b, orthologs of human CCR4 in Arabidopsis, have various physiological effects. AtCCR4 isoforms are likely to have specific target mRNAs related to each physiological effect; however, AtCCR4 does not have RNA-binding capability. Therefore, identifying factors that interact with AtCCR4a/b is indispensable to understand its function as a regulator of gene expression, as well as the target mRNA recognition mechanism. Here, we identified putative components of the AtCCR4-NOT complex using co-immunoprecipitation in combination with mass spectrometry using FLAG-tagged AtCCR4b and subsequent verification with a yeast two-hybrid assay. Interestingly, four of 11 AtCAF1 isoforms interacted with both AtCCR4b and AtNOT1, whereas two isoforms interacted only with AtNOT1 in yeast two-hybrid assays. These results imply that Arabidopsis has multiple CCR4-NOT complexes with various combinations of deadenylases. We also revealed that the RNA-binding protein Arabidopsis Pumilio 5 and 2 interacted with AtCCR4a/b in the cytoplasm with a few foci. - Involvement of the membrane-localized ubiquitin ligase ATL8 in the sugar starvation response in Arabidopsis
Luo Y; Aoyama S; Fukao Y; Chiba Y; Sato T; Yamaguchi J
Plant Biotechnology, 36, 2, 107, 112, Japanese Society for Plant Cell and Molecular Biology, 2019, [Peer-reviewed], [International Magazine]
English, Scientific journal - Sugar-responsive transcription factor bZIP3 affects leaf shape in Arabidopsis plants
Sanagi M; Lu Y; Aoyama S; Morita Y; Mitsuda N; Ikeda M; Ohme-Takagi M; Sato T; Yamaguchi J
Plant Biotechnology, 35, 2, 167, 170, Jun. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Sugars are essential for plant metabolism, growth and development. Plants must therefore manage their growth and developmental processes in response to sugar availability. Sugar signaling pathways constitute a complicated molecular network and are associated with global transcriptional regulation. However, the molecular mechanisms underlying sugar signaling remain largely unclear. This study reports that the protein basic-region leucine zipper 3 (bZIP3) is a novel sugar-responsive transcription factor in Arabidopsis plants. The expression of bZIP3 was rapidly repressed by sugar. Genetic analysis indicated that bZIP3 expression was modulated by the SNF1-RELATED KINASE 1 (SnRK1) pathway. Moreover, transgenic plants overexpressing bZIP3 and dominant repressor form bZIP3-SRDX showed aberrant shaped cotyledons with hyponastic bending. These findings suggest that bZIP3 plays a role in plant responses to sugars and is also associated with leaf development. - Methods for elucidation of plant senescence in response to C/N-nutrient balance
Shoki Aoyama; Junji Yamaguchi; Takeo Sato
Methods in Molecular Biology, 1744, 151, 159, Humana Press Inc., 2018, [Peer-reviewed], [Corresponding author], [International Magazine]
English, In book - Membrane-localized ubiquitin ligase ATL15 functions in sugar-responsive growth regulation in Arabidopsis
Shoki Aoyama; Saki Terada; Miho Sanagi; Yoko Hasegawa; Yu Lu; Yoshie Morita; Yukako Chiba; Takeo Sato; Junji Yamaguchi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 491, 1, 33, 39, Sep. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal - Ubiquitin related enzymes and plant-specific ubiquitin ligase ATL family in tomato plants
Xingwen Li; Yoko Hasegawa; Yu Lu; Takeo Sato
PLANT BIOTECHNOLOGY, 34, 2, 71, 78, Jun. 2017, [Peer-reviewed], [Corresponding author], [International Magazine]
English - Arabidopsis CBL-Interacting Protein Kinases Regulate Carbon/Nitrogen-Nutrient Response by Phosphorylating Ubiquitin Ligase ATL31
Shigetaka Yasuda; Shoki Aoyama; Yoko Hasegawa; Takeo Sato; Junji Yamaguchi
MOLECULAR PLANT, 10, 4, 605, 618, Apr. 2017, [Peer-reviewed], [International Magazine]
English, Scientific journal - Direct transcriptional activation of BT genes by NLP transcription factors is a key component of the nitrate response in Arabidopsis
Takeo Sato; Shugo Maekawa; Mineko Konishi; Nozomi Yoshioka; Yuki Sasaki; Haruna Maeda; Tetsuya Ishida; Yuki Kato; Junji Yamaguchi; Shuichi Yanagisawa
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 483, 1, 380, 386, Jan. 2017, [Peer-reviewed], [Lead author], [International Magazine]
English, Scientific journal - Characterization of ubiquitin ligase SlATL31 and proteomic analysis of 14-3-3 targets in tomato fruit tissue (Solanum lycopersicum L.)
Yu Lu; Shigetaka Yasuda; Xingwen Li; Yoichiro Fukao; Takayuki Tohge; Alisdair R. Fernie; Chiaki Matsukura; Hiroshi Ezura; Takeo Sato; Junji Yamaguchi
JOURNAL OF PROTEOMICS, 143, 254, 264, Jun. 2016, [Peer-reviewed], [Corresponding author], ［Internationally co-authored］, [International Magazine]
English, Scientific journal - Integration of C/N-nutrient and multiple environmental signals into the ABA signaling cascade
Yu Lu; Junji Yamaguchi; Takeo Sato
PLANT SIGNALING & BEHAVIOR, 10, 12, e1048940, Dec. 2015, [Peer-reviewed], [Corresponding author], [International Magazine]
English - ABI1 regulates carbon/nitrogen-nutrient signal transduction independent of ABA biosynthesis and canonical ABA signalling pathways in Arabidopsis (vol 66, pg 2763, 2015)
Yu Lu; Yuki Sasaki; Xingwen Li; Izumi C. Mori; Takakazu Matsuura; Takashi Hirayama; Takeo Sato; Junji Yamaguchi
JOURNAL OF EXPERIMENTAL BOTANY, 66, 15, 4851, 4851, Aug. 2015, [Peer-reviewed], [International Magazine]
English - ABI1 regulates carbon/nitrogen-nutrient signal transduction independent of ABA biosynthesis and canonical ABA signalling pathways in Arabidopsis
Yu Lu; Yuki Sasaki; Xingwen Li; Izumi C. Mori; Takakazu Matsuura; Takashi Hirayama; Takeo Sato; Junji Yamaguchi
JOURNAL OF EXPERIMENTAL BOTANY, 66, 9, 2763, 2771, May 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal - The Arabidopsis ubiquitin ligase ATL31 is transcriptionally controlled by WRKY33 transcription factor in response to pathogen attack
Thais Huarancca Reyes; Shugo Maekawa; Takeo Sato; Junji Yamaguchi
PLANT BIOTECHNOLOGY, 32, 1, 11, +, Mar. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal - Pale-Green Phenotype of atl31 atl6 Double Mutant Leaves Is Caused by Disruption of 5-Aminolevulinic Acid Biosynthesis in Arabidopsis thaliana
Shugo Maekawa; Atsushi Takabayashi; Thais Huarancca Reyes; Hiroko Yamamoto; Ayumi Tanaka; Takeo Sato; Junji Yamaguchi
PLOS ONE, 10, 2, e0117662, Feb. 2015, [Peer-reviewed], [International Magazine]
English, Scientific journal - Proteomic Analysis of the 26S Proteasome Reveals Its Direct Interaction with Transit Peptides of Plastid Protein Precursors for Their Degradation
Kaori Sako; Yuki Yanagawa; Tomoyuki Kanai; Takeo Sato; Motoaki Seki; Masayuki Fujiwara; Yoichiro Fukao; Junji Yamaguchi
JOURNAL OF PROTEOME RESEARCH, 13, 7, 3223, 3230, Jul. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal - Phosphorylation of Arabidopsis Ubiquitin Ligase ATL31 Is Critical for Plant Carbon/Nitrogen Nutrient Balance Response and Controls the Stability of 14-3-3 Proteins
Shigetaka Yasuda; Takeo Sato; Shugo Maekawa; Shoki Aoyama; Yoichiro Fukao; Junji Yamaguchi
JOURNAL OF BIOLOGICAL CHEMISTRY, 289, 22, 15179, 15193, May 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal - Regulation of senescence under elevated atmospheric CO2 via ubiquitin modification
Shoki Aoyama; Yu Lu; Junji Yamaguchi; Takeo Sato
Plant Signaling and Behavior, 9, 5, e28839, Landes Bioscience, 16 Apr. 2014, [Peer-reviewed], [Corresponding author], [International Magazine]
English - The Carbon/Nitrogen Regulator ARABIDOPSIS TOXICOS EN LEVADURA31 Controls Papilla Formation in Response to Powdery Mildew Fungi Penetration by Interacting with SYNTAXIN OF PLANTS121 in Arabidopsis
Shugo Maekawa; Noriko Inada; Shigetaka Yasuda; Yoichiro Fukao; Masayuki Fujiwara; Takeo Sato; Junji Yamaguchi
PLANT PHYSIOLOGY, 164, 2, 879, 887, Feb. 2014, [Peer-reviewed], [International Magazine]
English, Scientific journal - Ubiquitin Ligase ATL31 Functions in Leaf Senescence in Response to the Balance Between Atmospheric CO2 and Nitrogen Availability in Arabidopsis
Shoki Aoyama; Thais Huarancca Reyes; Lorenzo Guglielminetti; Yu Lu; Yoshie Morita; Takeo Sato; Junji Yamaguchi
PLANT AND CELL PHYSIOLOGY, 55, 2, 293, 305, Feb. 2014, [Peer-reviewed], [Corresponding author], ［Internationally co-authored］, [International Magazine]
English, Scientific journal - Assay for proteasome-dependent protein degradation and ubiquitinated proteins
Takeo Sato; Kaori Sako; Junji Yamaguchi
Methods in Molecular Biology, 1072, 655, 663, Humana Press Inc., 2014, [Peer-reviewed], [Lead author], [International Magazine]
English, Scientific journal - The Arabidopsis transcriptional repressor ERF9 participates in resistance against necrotrophic fungi
Yosuke Maruyama; Natsuko Yamoto; Yuya Suzuki; Yukako Chiba; Ken-ichi Yamazaki; Takeo Sato; Junji Yamaguchi
PLANT SCIENCE, 213, 79, 87, Dec. 2013, [Peer-reviewed], [International Magazine]
English, Scientific journal - Proteomics Analysis Reveals a Highly Heterogeneous Proteasome Composition and the Post-translational Regulation of Peptidase Activity under Pathogen Signaling in Plants
Hui H. Sun; Yoichiro Fukao; Sakiko Ishida; Hiroko Yamamoto; Shugo Maekawa; Masayuki Fujiwara; Takeo Sato; Junji Yamaguchi
JOURNAL OF PROTEOME RESEARCH, 12, 11, 5084, 5095, Nov. 2013, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal - The Arabidopsis ubiquitin ligases ATL31 and ATL6 control the defense response as well as the carbon/nitrogen response
Shugo Maekawa; Takeo Sato; Yutaka Asada; Shigetaka Yasuda; Midori Yoshida; Yukako Chiba; Junji Yamaguchi
PLANT MOLECULAR BIOLOGY, 79, 3, 217, 227, Jun. 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal - Arabidopsis RPT2a, 19S Proteasome Subunit, Regulates Gene Silencing via DNA Methylation
Kaori Sako; Yuko Maki; Tomoyuki Kanai; Eriko Kato; Shugo Maekawa; Shigetaka Yasuda; Takeo Sato; Masaaki K. Watahiki; Junji Yamaguchi
PLOS ONE, 7, 5, e37086, May 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal - Proteomics approach to study metabolic regulation mediated by ubiquitin-proteasome system in Arabidopsis
Yasuda S; Maekawa S; Sato T; Yamaguchi J
Frontiers of Agriculture Proteome Research, 39, 44, 2012, [Peer-reviewed], [International Magazine] - Sugar-inducible RPT2a, a subunit of 26S proteasome, participates in sugar response in Arabidopsis
Huihui Sun; Kaori Sako; Yuya Suzuki; Shugo Maekawa; Shigetaka Yasuda; Yukako Chiba; Takeo Sato; Junji Yamaguchi
PLANT BIOTECHNOLOGY, 29, 3, 279, 284, 2012, [Peer-reviewed], [International Magazine]
English, Scientific journal - Identification of 14-3-3 proteins as a target of ATL31 ubiquitin ligase, a regulator of the C/N response in Arabidopsis
Takeo Sato; Shugo Maekawa; Shigetaka Yasuda; Yukie Domeki; Kuni Sueyoshi; Masayuki Fujiwara; Yoichiro Fukao; Derek B. Goto; Junji Yamaguchi
PLANT JOURNAL, 68, 1, 137, 146, Oct. 2011, [Peer-reviewed], [Lead author], [International Magazine]
English, Scientific journal - Carbon and nitrogen metabolism regulated by the ubiquitin-proteasome system
Takeo Sato; Shugo Maekawa; Shigetaka Yasuda; Junji Yamaguchi
Plant Signaling and Behavior, 6, 10, 1465, 1468, Landes Bioscience, 2011, [Peer-reviewed], [Lead author], [International Magazine]
English, Scientific journal - Studies on Plant Ubiquitin-Proteasome System by Proteomics Approaches—our Case Study on Metabolic Regulation—
Yamaguchi Junji; Satoh Takeo
Abstracts for Annual Meeting of Japanese Proteomics Society, 2011, 64, 64, Japanese Proteomics Society (Japan Human Proteome Organisation), 2011, [Domestic magazines]
Japanese - CNI1/ATL31, a RING-type ubiquitin ligase that functions in the carbon/nitrogen response for growth phase transition in Arabidopsis seedlings
Takeo Sato; Shugo Maekawa; Shigetaka Yasuda; Yutaka Sonoda; Etsuko Katoh; Takanari Ichikawa; Miki Nakazawa; Motoaki Seki; Kazuo Shinozaki; Minami Matsui; Derek B. Goto; Akira Ikeda; Junji Yamaguchi
PLANT JOURNAL, 60, 5, 852, 864, Dec. 2009, [Peer-reviewed], [Lead author], [International Magazine]
English, Scientific journal - DEAR1, a transcriptional repressor of DREB protein that mediates plant defense and freezing stress responses in Arabidopsis
Tomokazu Tsutsui; Wataru Kato; Yutaka Asada; Kaori Sako; Takeo Sato; Yutaka Sonoda; Satoshi Kidokoro; Kazuko Yamaguchi-Shinozaki; Masanori Tamaoki; Keita Arakawa; Takanari Ichikawa; Miki Nakazawa; Motoaki Seki; Kazuo Shinozaki; Minami Matsui; Akira Ikeda; Junji Yamaguchi
JOURNAL OF PLANT RESEARCH, 122, 6, 633, 643, Nov. 2009, [Peer-reviewed], [International Magazine]
English, Scientific journal - SHA1, a novel RING finger protein, functions in shoot apical meristem maintenance in Arabidopsis
Yutaka Sonoda; Shan-Guo Yao; Kaori Sako; Takeo Sato; Wataru Kato; Masa-aki Ohto; Takanari Ichikawa; Minami Matsui; Junji Yamaguchi; Akira Ikeda
PLANT JOURNAL, 50, 4, 586, 596, May 2007, [Peer-reviewed], [International Magazine]
English, Scientific journal
- たん水直播栽培の効率化
佐藤長緒, ニューカントリー, 818, 18, 20, May 2022, [Lead author, Corresponding author] - 乳酸菌培養液由来バイオスティミュラントの開発
眞木祐子; 佐藤長緒, バイオスティミュラントハンドブック, 399, 403, Apr. 2022 - P022 Anthranilic acid and its derivatives promote rooting specifically to adventitious root
Maki Yuko; Soejima Hiroshi; Sato Takeo; Watahiki Masaaki; Yamaguchi Junji, 植物化学調節学会研究発表記録集, 50, 40, 40, 01 Oct. 2015
The Japanese Society for Chemical Regulation of Plants, Japanese - アントラニル酸および誘導体は不定根特異的発根促進活性を持つ
眞木祐子; 副島洋; 佐藤長緒; 綿引雅昭; 山口淳二, 植物の生長調節, 50, Supplement, 40, 01 Oct. 2015
Japanese - 4-1-7 シロイヌナズナにおける硝酸シグナル応答型転写因子NLPの新規標的遺伝子BT群の機能解析(4-1 植物の多量栄養素,2015年度京都大会)
前川 修吾; 吉岡 希; 小西 美稲子; 石田 哲也; 加藤 祐樹; 佐々木 勇樹; 佐藤 長緒; 山口 淳二; 柳澤 修一, 日本土壌肥料学会講演要旨集, 61, 50, 50, 09 Sep. 2015
一般社団法人日本土壌肥料学会, Japanese - 4-1-9 植物の炭素/窒素栄養バランス応答を制御する膜局在型ユビキチンリガーゼATL31の機能解析(4-1 植物の多量栄養素,2015年度京都大会)
佐藤 長緒; HUARANCCA REYES Thais; 植村 知博; 深尾 陽一朗; 山口 淳二, 日本土壌肥料学会講演要旨集, 61, 50, 50, 09 Sep. 2015
一般社団法人日本土壌肥料学会, Japanese - 4-1-10 大気中二酸化炭素と窒素栄養バランスに応答した老化制御におけるユビキチンリガーゼATL31の機能解析(4-1 植物の多量栄養素,2015年度京都大会)
青山 翔紀; Reyes Thais Huarancca; Guglielminetti Lorenzo; 陸 宇; 森田 嘉恵; 佐藤 長緒; 山口 淳二, 日本土壌肥料学会講演要旨集, 61, 51, 51, 09 Sep. 2015
一般社団法人日本土壌肥料学会, Japanese - 植物免疫における膜局在型ユビキチンリガーゼATL31のリン酸化とその機能解析
安田盛貴; 長谷川陽子; 門田康弘; 深尾陽一朗; 佐藤長緒; 山口淳二, 日本植物生理学会年会要旨集, 56th, 2015 - Exploration of substrates for a membrane-localized ubiquitin ligase in plant innate immunity
安田盛貴; 長谷川陽子; 門田康弘; 深尾陽一朗; 佐藤長緒; 山口淳二, 日本プロテオーム学会大会プログラム・抄録集, 2015 (Web), 2015 - C/Nバランス調節による植物の代謝・成長戦略
佐藤長緒; 山口淳二, 化学と生物, 51, 11, 763, 772, 2013
Japan Society for Bioscience, Biotechnology, and Agrochemistry, Japanese - Ring-type ubiquitin ligase and proteasome function in plants
Shugo Maekawa; Kaori Sako; Takeo Sato, Seikagaku, 84, 6, 416, 424, 2012
Japanese, Book review - ユビキチンカスケード-プロテアソームシステムによる植物の栄養応答制御 : C/Nバランス応答機構解明の新たな糸口
佐藤 長緒, 化学と生物, 47, 1, 2, 4, 01 Jan. 2009
社団法人 日本農芸化学会, Japanese - ユビキチンカスケードを視点とした高等植物の"無限成長" : 茎頂分裂組織の機能維持に必要な能動的タンパク質分解システム
池田 亮; 佐藤 長緒, 化学と生物, 46, 8, 516, 517, 01 Aug. 2008
社団法人 日本農芸化学会, Japanese
- 生命システム科学基礎論, 2024年, 修士課程, 生命科学院
- 環境応答システム科学特論, 2024年, 修士課程, 生命科学院
- 細胞生物学概論, 2024年, 学士課程, 理学部
- 科学・技術の世界（1単位）, 2024年, 学士課程, 全学教育
- 生物学Ⅰ, 2024年, 学士課程, 全学教育
- ISP生物科学実習Ⅱ・a, 2024年, 学士課程, 理学部
- ISP生物科学実習Ⅱ・b, 2024年, 学士課程, 理学部
- 形態機能学実習, 2024年, 学士課程, 理学部
- 形態機能学実習, 2024年, 学士課程, 理学部
- 形態機能学Ⅱ, 2024年, 学士課程, 理学部
- 形態機能学b, 2024年, 学士課程, 理学部
- 形態機能学Ⅱ, 2024年, 学士課程, 理学部
- 生物学Ⅰ, 2024年, 学士課程, 全学教育
- 機能生物学Ⅲ, 2024年, 学士課程, 理学部
- Symplast; intercellular communication mechanism in plants under environmental changes
Grants-in-Aid for Scientific Research
01 Apr. 2025 - 31 Mar. 2030
野田口 理孝; 松林 嘉克; 児玉 豊; 豊岡 公徳; 吉田 聡子; 打田 直行; 多田 安臣; 壽崎 拓哉; 鈴木 孝征; 佐藤 長緒; 太治 輝昭; 野元 美佳; 芦苅 基行
Japan Society for the Promotion of Science, Grant-in-Aid for Transformative Research Areas (A), Kyoto University, 25H01340 - 植物のシンプラスト栄養輸送を介した個体成長統御機構
科学研究費助成事業
01 Apr. 2025 - 31 Mar. 2030
佐藤 長緒; 木下 俊則
日本学術振興会, 学術変革領域研究(A), 北海道大学, 25H01346 - 植物の細胞内エネルギーセンサーを介した環境に応じた免疫活性制御機構
科学研究費助成事業
01 Apr. 2026 - 31 Mar. 2029
佐藤 長緒; 眞木 美帆
日本学術振興会, 基盤研究(B), 北海道大学, 26K01704 - 不規則な栄養環境おける植物ステージゲート機構
科学研究費助成事業
01 Apr. 2023 - 31 Mar. 2025
佐藤 長緒
日本学術振興会, 学術変革領域研究(A), 北海道大学, 23H04184 - 栄養シグナルによる膜交通制御と植物成長最適化機構の解明
科学研究費助成事業 基盤研究(B)
01 Apr. 2021 - 31 Mar. 2024
山口 淳二; 佐藤 長緒; 高木 純平
日本学術振興会, 基盤研究(B), 北海道大学, 21H02150 - マメ科植物の根粒形成と成長相転換制御における栄養シグナル伝達系の役割
形質転換植物デザイン研究拠点共同利用・共同研究課題
Apr. 2023 - Mar. 2024
高木 純平; 佐藤 長緒; 眞木 美帆; 久保 晁生
国立大学法人筑波大学つくば機能植物イノベーション研究センター, 一般型・若手, 北海道大学, 2304 - 窒素栄養欠乏に応答した植物ステージゲート制御機構の全容解明
科学研究費助成事業 学術変革領域研究(A)
10 Sep. 2021 - 31 Mar. 2023
佐藤 長緒
本研究では，植物が異なる時間スケールの栄養欠乏ストレスに対して誘導する応答について，その制御機構の解明を目指している。最近申請者らは，栄養素の中でも特に重要な窒素に着目し，窒素応答性花成制御を担う鍵因子として転写因子FBH4および上流キナーゼSnRK1を同定し，長年謎であった窒素欠乏による花成早期化の分子機構の一端を明らかにした。本研究では，こうした鍵因子の機能に着目し，植物が細胞内における短期的な栄養欠乏応答から長期的・個体レベルでの応答段階へと移行する際の分子スイッチ機構について解明を目指す。主に，下記3研究課題に関する詳細な解析を効率的に実施する。
研究課題 1）短期的および長期的な窒素欠乏に応じたFBH4機能変換機構の解析
研究課題 2）窒素応答性成長相転換を誘導する上流シグナル機構の解析
研究課題 3）成長相転換制御における光周期シグナルと窒素シグナル統合機構の解析
当該年度は，ChIP-seq解析に取組み，FBH4が制御する標的遺伝子についてグローバルな情報を得た。また，これまであまり分かっていなかった窒素欠乏におけるエピゲノムシグナルの変動についても新たな知見が得られた。また，これまでのIP-MS解析で得られた情報からFBH4機能とリン酸化修飾や相互作用因子について解析した。加えて，上流キナーゼSnRK1の活性変動について，窒素欠乏時の代謝物変動との関連を解析し，どういった代謝物がSnRK1活性制御に影響するかについてin vivoおよびin vitroでの解析が可能となった。
日本学術振興会, 学術変革領域研究(A), 北海道大学, 21H05644 - ユビキチンシグナルによる膜交通制御を介した植物の病原体抵抗性強化機構
科学研究費助成事業 基盤研究(C)
01 Apr. 2020 - 31 Mar. 2023
佐藤 長緒
本研究では，植物の病原体ストレス抵抗性に着目し，その優れた環境適応能力を支える細胞内膜交通系の分子実態と制御機構について明らかにする。膜交通系は，細胞膜上の環境シグナル受容体・伝達因子や輸送体の機能制御に重要な役割を果たす。病原体抵抗性においては，細胞壁の強化が重要であり，膜交通系はその一翼を担っている。本研究では特に，膜局在型ユビキチンリガーゼによる膜交通因子の機能制御に注目，ユビキチンシグナルの果たす役割について，新たな視点で解析を実施する。主に，下記3研究課題に関する詳細な解析を効率的に実施する。
1．病原体ストレスに応じたユビキチンリガーゼ活性の変動制御機構の解析
2. 細胞壁成分の輸送に関わる構成因子と積荷の解析
3. 鍵因子機能改変株を用いた病原体抵抗性解析
当該年度は，TGN/EE局在型SNARE因子と膜局在型ユビキチンリガーゼATL31の関係性について解析を行った。その結果，TGN/EE局在型SNARE因子と膜局在型ユビキチンリガーゼATL31が相互作用していること，TGN/EE局在型SNARE因子がATL31の細胞内局在制御に重要な役割を果たすことを見出した。また，遺伝学的な解析も行い，TGN/EE局在型SNARE因子がATL31の機能発現に重要であることが示唆された。これまでの研究から，TGN/EE局在型SNARE因子のユビキチン化部位等の情報が得られていることから，このユビキチン化候補部位に変異を導入した変異型SNARE因子を発現する植物の作出を進めている。
日本学術振興会, 基盤研究(C), 北海道大学, 20K05949 - マメ科植物の根粒形成と成長相転換制御における栄養シグナル伝達系の役割
「形質転換植物デザイン研究拠点」共同利用・共同研究
Apr. 2022 - Mar. 2023
高木 純平; 佐藤 長緒; 眞木 美帆; 久保 晁生
国立大学法人筑波大学つくば機能植物イノベーション研究センター, 一般型・若手, 北海道大学, 2201 - ユビキチン修飾による病原体の認識受容と機能発現制御機構の全容解明
科学研究費補助金（基盤研究（B））
Apr. 2018 - Mar. 2021
山口淳二
文部科学省, Competitive research funding - ユビキチンシグナルによる膜交通制御と環境ストレス適応機構の解明
科学研究費補助金(基盤研究(C))
Apr. 2017 - Mar. 2020
佐藤長緒
文部科学省, Principal investigator, Competitive research funding - C/N栄養シグナルと花成制御シグナル統合分子基盤の解明
科学研究費補助金(若手研究(Ｂ))
Apr. 2015 - Mar. 2017
佐藤長緒
文部科学省, Principal investigator, Competitive research funding - 膜局在型植物ユビキチンリガーゼATL31による環境シグナル伝達調節の包括的解明
科学研究費補助金(基盤研究(B))
Apr. 2014 - Mar. 2017
山口淳二
文部科学省, Competitive research funding - Regulatory mechanism of nutrient signaling and metabolism in plants
Grants-in-Aid for Scientific Research
Apr. 2012 - Mar. 2014
SATO Takeo
Nutrient availability, in particularly the balance of carbon (C) and nitrogen (N) is one of the most important factors for regulating plant metabolism and development. Our proteomics and biochemical analysis demonstrated that the ATL31 targets 14-3-3 proteins for ubiquitination and regulates the 14-3-3s stabilities in response to C/N status. Furthermore, we revealed that the targeting of ATL31 to 14-3-3 is dependent on phosphorylation of specific Ser/Thr residues in C-terminal region of ATL31. Our proteome and metabolome analysis demonstrated the dynamics of 14-3-3 interactors and regulation of primary metabolism via phosphorylation cascade in response to C/N condition.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, Principal investigator, Competitive research funding, 24770035 - 植物の栄養応答制御に関与するユビキチン‐プロテアソームシステムの全容解明
科学研究費助成事業
2009 - 2010
佐藤 長緒
様々な栄養素の中でも,基幹代謝の根幹を担う炭素源(C)と窒素源(N)の獲得は重要であり,これらは代謝で密に関わり合っている。そのため植物は,CとNの相対量比「C/N」を感知し適応する能力を有している。申請者らは,未解明であった植物C/N応答機構の解明を目指し研究を進めている。当該年度は昨年度に引き続き,新規C/N応答制御因子であるユビキチンリガーゼATL31の機能解析を行い,ユビキチン-プロテアソームシステム(UPS)による植物C/N応答制御機構の実態を明らかにした。
1.ユビキチンリガーゼATL31と14-3-3タンパク質相互作用を介したC/N応答制御機構の発見
昨年度までの研究でユビキチンリガーゼATL31の相互作用因子として14-3-3タンパク質が単離されていた。14-3-3はC,N代謝に関わる多くの酵素活性を制御する重要な因子である。本年度は生化学および遺伝学的な解析を通して,ATL31がin vivoで14-3-3と直接結合しユビキチン化すること,また14-3-3タンパク質の安定性がC/Nに応じてATL31及びプロテアソーム活性依存的に制御されることが示された。さらに,新たに作製した14-3-3過剰発現体がATL31過剰発現体とは逆にC/Nストレス過剰応答性を示すことがわかった。よって,ATL31はUPSによる14-3-3の分解を介して,植物C/N応答を制御するという新規のメカニズムを明らかにした。
2.ATL31が制御する植物C/N代謝ネットワークの解析
理研・植物科学センターとの共同研究で,C/Nストレス下における野生型及びatl31機能欠損変異体のメタボローム解析を行った。これによりATL31が実際に制御している代謝レベルでの制御ターゲットに関する知見が得られた。現在,各代謝過程に関わる酵素の活性変化に関する検証も進めている。
日本学術振興会, 特別研究員奨励費, 北海道大学, 09J01575
