Researcher basic information

• 博士（医学）, 北海道大学

• https://researchmap.jp/sudo?lang=en

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201301096887048795
• https://www.hokudaiseikei.jp/pdf/researchmap202508.pdf

• 201301096887048795

• 再生医療
• 生体材料・生体力学
• 人工知能・情報科学
• 医工連携・産学連携
• 整形外科・脊椎脊髄外科
• 脊柱変形矯正
• 遺伝子治療・幹細胞治療

• Life Science, Orthopedics, 脊椎脊髄病学

Research activity information

• May 2023, 第96回日本整形外科学会学術総会最優秀口演賞（研究責任者）
  筌場大介
• Nov. 2022, 第56回日本側彎症学会学術集会 Short Symposium Award
  須藤英毅
• Oct. 2022, 第37回日本整形外科学会基礎学術集会最優秀演題賞（研究責任者）
  鈴木久崇
• Sep. 2022, 第71回東日本整形災害外科学会若手優秀演題アウォｰド最優秀賞（研究責任者）
  鈴木久崇
• Jul. 2022, Computer-Aided Design and Applications, Annual CAD Conference, Computer-Aided Design and Applications, Annual CAD Conference Best Paper Award
  Ayane Soutome;Satoshi Kanai;Hiroaki Date;Terufumi Kokabu;Yuichiro Abe;Hiroshi Moridaira;Hiroshi Taneichi;Hideki Sudo
• Apr. 2022, 日本脊椎脊髄病学会奨励賞（論文責任著者）
  筌場大介、山田勝久、辻本武尊、浦勝郎、野々山貴行、岩崎倫政、須藤英毅
• Dec. 2021, （厚生労働省、第一生命、朝日新聞厚生文化事業団、ＮＨＫ厚生文化事業団）, 保健文化賞
  須藤英毅
• Sep. 2021, North American Spine Society 2021 Best Paper Award (corresponding author)
  Kokabu T, Kanai S, Kawakami N, Uno K, Kotani T, Suzuki T, Tachi H, Abe Y, Iwasaki N, Sudo H.
• Mar. 2021, 日本軟骨代謝学会賞（論文責任著者）
  Bone marrow mesenchymal stem cells combined with ultra-purified alginate gel as a regenerative therapeutic strategy after discectomy for degenerated intervertebral discs
  筌場大介、須藤英毅、辻本武尊、浦勝郎、山田勝久、岩崎倫政
• Apr. 2020, 日本脊椎脊髄病学会奨励賞（論文責任著者）
  Caspase-3 knockout inhibits intervertebral disc degeneration related to injury but accelerates degeneration related to aging
  大西貴士,山田勝久,岩崎浩司,辻本武尊,東秀明,木村太一,岩崎倫政,須藤英毅
• Apr. 2020, 文部科学大臣表彰科学技術賞（研究部門）
  脊柱変形疾患に対する革新的医療機器の開発と実用化研究
  須藤英毅(筆頭者)、金井理、千葉晶彦
• Mar. 2020, 日本軟骨代謝学会賞（論文責任著者）
  An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair: A preclinical proof-of-concept study
  辻本武尊;須藤英毅;東藤正浩;山田勝久;岩崎浩司;大西貴士;廣浜直樹;野々山貴行;筌場大介;浦勝郎;伊藤陽一;岩崎倫政
• Nov. 2018, Best Paper Award (17th International Conference on Precision Engineering)
  3D Asymmetry Analysis of Human’s Back Surface for Early Screening of Idiopathic Scoliosis
  Satoshi Kanai;Hideki Sudo;Terufumi Kokabu;Hiroshi Nagaeda;Takayuki Hayashi;Hajime Ohta
• Feb. 2018, ORS/OREF Travel Grant in Orthopaedic Research Translation (Corresponding author, Orthopaedic Research Society, United States, 2018)
  The role of caspase 3 gene in intervertebral disc degeneration due to injury and aging
  Takashi Ohnishi;Hideki Sudo;Takeru Tsujimoto;Norimasa Iwasaki
• Feb. 2018, ORS/OREF Travel Grant in Orthopaedic Research Translation (Corresponding author, Orthopaedic Research Society, United States, 2018)
  Acellular Ultra-purified Alginate Gels for Intervertebral Disc Regeneration in a Preclinical Animal Model
  Takeru Tsujimoto;Hideki Sudo;Masahiro Todoh;Katsuhisa Yamada;Koji Iwasaki;Takashi Ohnishi;Norimasa Iwasaki
• Feb. 2017, Force & Motion Foundation/ORS Young Scientist Travel Grant (Orthopaedic Research Society, USA, Corresponding author)
  Effects of multilevel facetectomy and screw density on postoperative changes in spinal rod contour in thoracic adolescent idiopathic scoliosis surgery
  Kokabu Terufumi, Hideki Sudo, Yuichiro Abe, Manabu Ito, Norimasa Iwasaki
• 2017, Biomaterials Science Presentation Prize (Royal Society of Chemistry)
  須藤英毅
• 2017, 日本バイオマテリアル学会ハイライト講演受賞
  須藤英毅
• Jan. 2017, 北海道大学研究総長賞
  須藤英毅
• 2016, AOSpine Research Grant助成
  須藤英毅
• 2016, 北海道大学医学部医学科エクセレント・ティーチャー（優秀賞）
  須藤英毅
• Feb. 2015, 北海道科学技術奨励賞
  須藤英毅
• 2015, John Moe Award (Scoliosis Research Society, USA)
  須藤英毅
• Nov. 2014, 日本医師会医学研究奨励賞
  須藤英毅
• 2012, 財団法人博慈会老人病研究所優秀論文賞（基礎部門）
  須藤英毅
• 2011, The top scored ORS poster within Spine posters (Orthopaedic Research Society, United States)
  須藤英毅
• 2011, 第6回Asia Traveling Fellowship (日本脊椎脊髄病学会)
  須藤英毅
• 2008, 整形災害外科学研究助成財団研究助成エーザイ奨励賞
  須藤英毅
• 2005, 北海道大学医学部高桑榮松奨学基金奨励賞
  須藤英毅
• 2005, 北海道整形災害外科学会学術奨励賞
  須藤英毅

• Feasibility and accuracy of AI-assisted 3D MRI/CT fusion imaging for preoperative simulation of full-endoscopic transforaminal discectomy.
  Daisuke Ukeba; Ken Nagahama; Yoshinori Hyugaji; Katsuhisa Yamada; Hideki Sudo; Tsutomu Endo; Takashi Ohnishi; Ryo Fujita; Yoshinao Koike; Yohei Sodeyama; Norimasa Iwasaki
  European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, 35, 3, 1077, 1084, Mar. 2026, [International Magazine]
  English, Scientific journal
• Lumbar ossification of the posterior longitudinal ligament as a distinct phenotype of diffuse spinal ligament ossification1.
  Yoshinao Koike; Tsutomu Endo; Ryo Fujita; Katsuhisa Yamada; Masahiro Kanayama; Hideki Sudo; Ken Kadoya; Huohuo Xue; M Alaa Terkawi; Daisuke Ukeba; Takashi Ohnishi; Shotaro Fukada; Yohei Sodeyama; Ryota Suzuki; Misaki Ishii; Norimasa Iwasaki
  The spine journal : official journal of the North American Spine Society, 03 Feb. 2026, [International Magazine]
  English, Scientific journal, BACKGROUND CONTEXT: Lumbar ossification of the posterior longitudinal ligament (L-OPLL) has been underrecognized and remains poorly characterized clinically. We hypothesized that L-OPLL constitutes a distinct phenotype within the broader OPLL spectrum, sharing features of obesity and diffuse spinal ligament ossification. PURPOSE: To evaluate the clinical and radiographic features of L-OPLL and assess their relationship with diffuse spinal ligament ossification and obesity-related factors. STUDY DESIGN: Cross-sectional study with a replication cohort. PATIENT SAMPLE: A total of 186 patients with OPLL were diagnosed using whole-spine computed tomography (CT) at a regional spine center in Japan (2007-2024). Additionally, 75 asymptomatic individuals with OPLL from a population-based health screening cohort comprised the replication cohort. OUTCOME MEASURES: Patient background, including BMI, was assessed. Spinal ligament ossification was evaluated using whole-spine CT. The severity of ossification was scored for four ligaments-OPLL, OALL (ossification of the anterior longitudinal ligament), OLF (ossification of the ligamentum flavum), and ossification of the supra/interspinous ligament-and summed to define the ossification index (OS index). Regional scores from the cervical, thoracic, and lumbar spine were combined to calculate the total index. METHODS: In the primary analysis, patients were classified into L-OPLL and non-L-OPLL groups, and their clinical and radiographic features were compared. Multiple linear regression analysis was used to assess the independent association between L-OPLL and OS index. In the secondary analysis, patients were classified into three groups: localized cervical OPLL (C-OPLL), thoracic OPLL (T-OPLL), and L-OPLL groups, and comparisons were made between the localized C-OPLL group and the T- and L-OPLL groups. RESULTS: The L-OPLL group had a significantly higher BMI (median 27.5 vs. 26.0 kg/m², p=0.003) and greater prevalence of obesity than the non-L-OPLL group, along with significantly elevated thoracic OPLL and OLF indices. Multiple linear regression analysis confirmed that L-OPLL was independently associated with a higher OS index (regression coefficient: 0.448, 95% confidence interval: 0.162 to 0.735, p=0.002). The L-OPLL group also exhibited significantly higher BMI and OS index than the localized C-OPLL group, primarily driven by increased thoracic and lumbar OPLL and OLF. The replication cohort results were consistent with an association between L-OPLL, obesity, and diffuse ligament ossification. CONCLUSIONS: L-OPLL is rarely an isolated lumbar lesion; instead, it commonly coexists with extensive spinal ligament ossification and marked obesity. Its distinct clinical and radiographic features support classification as a separate entity within the broader OPLL spectrum.
• Full-Endoscopic Spine Surgery for Adjacent Segment Disease Using Artificial Intelligence-Assisted 3-Dimensional MRI/CT Fusion Imaging: A Case Report.
  Koyo Koizumi; Daisuke Ukeba; Ken Nagahama; Katsuhisa Yamada; Hideki Sudo; Norimasa Iwasaki
  JBJS case connector, 16, 1, 01 Jan. 2026, [International Magazine]
  English, Scientific journal, CASE: An 82-year-old woman presented with right medial thigh pain. Her medical history included multiple lumbar decompression and fusion surgeries. She was diagnosed with L3 radiculopathy secondary adjacent segment disease (ASD) after lumbar fusion. Full-endoscopic spine surgery (FESS) was performed for targeted decompression. Preoperative planning was conducted using AI-assisted 3-dimensional (3D) magnetic resonance imaging (MRI)/computed tomography (CT) fusion images. The procedure was completed under local anesthesia, resulting in postoperative pain relief. At the 3-year follow-up, the patient remained asymptomatic with no symptom recurrence. CONCLUSION: FESS, combined with detailed surgical planning using artificial intelligence -assisted 3D MRI/CT fusion imaging, may be an effective treatment strategy for ASD.
• Only One Occurrence of Postoperative White Cord Syndrome in Sequential 244 Cases of Cervical Spine Laminoplasty in 20 Years in a Single Institute.
  Masahiko Chikuma; Takashi Ohnishi; Hideki Sudo; Katsuhisa Yamada; Tsutomu Endo; Daisuke Ukeba; Hiroyuki Tachi; Yuichi Hasegawa; Toshiya Chubachi; Masahiko Takahata; Norimasa Iwasaki
  Clinical spine surgery, 17 Nov. 2025, [International Magazine]
  English, Scientific journal, STUDY DESIGN: A retrospective cross-sectional clinical study. OBJECTIVE: The purpose of the present study was to investigate the incidence of White cord syndrome (WCS) after laminoplasty (LAP) of the Kirita-Miyazaki method in 20 years. SUMMARY OF BACKGROUND DATA: Sequential 244 cases of cervical LAP in 20 years in a single institute. METHODS: We identified cases performed LAP for cervical myelopathy, followed by an independent record review for more precise inclusion. The primary target was the WCS, but we also collected other minor complications and obtained their incidence. RESULTS: We discovered one WCS case of 244 cases of cervical LAP (0.4%). The case was a 62-year-old man with diffuse ossification of the posterior longitudinal ligament causing very severe spinal cord compression. Other complications were dura mater tear in five cases (2%); C5 palsy in 19 cases (7.8%); C6 palsy in four cases (1.6%); sensation disorder in four cases (1.6%); surgical site infection in two cases (0.8%); epidural hematoma in one case (0.4%); incomplete decompression at the edge of LAP in one case (0.4%); occurrence of flexion myelopathy in one case (0.4%). CONCLUSIONS: The incidence of WCS secondary to cervical spine LAP was as low as one per 4880 person-years, lower than those of previous studies, while the rates of other typical complications were comparable. LEVEL OF EVIDENCE: Level 3.
• Prediction of cervical spondylotic myelopathy from a plain radiograph using deep learning with convolutional neural networks.
  Hiroyuki Tachi; Terufumi Kokabu; Hisataka Suzuki; Yoko Ishikawa; Akito Yabu; Yasushi Yanagihashi; Takahiko Hyakumachi; Tomohiro Shimizu; Tsutomu Endo; Takashi Ohnishi; Daisuke Ukeba; Hideki Sudo; Katsuhisa Yamada; Norimasa Iwasaki
  European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, 34, 9, 3786, 3797, Sep. 2025, [International Magazine]
  English, Scientific journal, PURPOSE: This study aimed to develop deep learning algorithms (DLAs) utilising convolutional neural networks (CNNs) to classify cervical spondylotic myelopathy (CSM) and cervical spondylotic radiculopathy (CSR) from plain cervical spine radiographs. METHODS: Data from 300 patients (150 with CSM and 150 with CSR) were used for internal validation (IV) using five-fold cross-validation strategy. Additionally, 100 patients (50 with CSM and 50 with CSR) were included in the external validation (EV). Two DLAs were trained using CNNs on plain radiographs from C3-C6 for the binary classification of CSM and CSR, and for the prediction of the spinal canal area rate using magnetic resonance imaging. Model performance was evaluated on external data using metrics such as area under the curve (AUC), accuracy, and likelihood ratios. RESULTS: For the binary classification, the AUC ranged from 0.84 to 0.96, with accuracy between 78% and 95% during IV. In the EV, the AUC and accuracy were 0.96 and 90%, respectively. For the spinal canal area rate, correlation coefficients during five-fold cross-validation ranged from 0.57 to 0.64, with a mean correlation of 0.61 observed in the EV. CONCLUSION: DLAs developed with CNNs demonstrated promising accuracy for classifying CSM and CSR from plain radiographs. These algorithms have the potential to assist non-specialists in identifying patients who require further evaluation or referral to spine specialists, thereby reducing delays in the diagnosis and treatment of CSM.
• Comparative Study between Full-Endoscopic Transforaminal Approach Lumbar Interbody Fusion (TF-LIF) Using PETLIF System and Minimally Invasive TLIF for Degenerative Lumbar Spine Disorders.
  Katsuhisa Yamada; Ken Nagahama; Hisataka Suzuki; Yuichiro Abe; Shigeto Hiratsuka; Yohei Sodeyama; Daisuke Ukeba; Hiroyuki Tachi; Tsutomu Endo; Takashi Ohnishi; Yuichi Hasegawa; Hideki Sudo; Takahiko Hyakumachi; Norimasa Iwasaki
  Spine surgery and related research, 9, 4, 460, 468, 27 Jul. 2025, [Domestic magazines]
  English, Scientific journal, INTRODUCTION: To compare the clinical outcomes between a full-endoscopic transforaminal approach lumbar interbody fusion (TF-LIF) using the percutaneous endoscopic transforaminal lumbar interbody fusion (PETLIF) system and a minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF). METHODS: A total of 102 patients (80 females, 22 males; mean age: 70.0 years) with degenerative lumbar spine disorders who underwent PETLIF and were followed up for 2 years were assigned to the PETLIF group. Based on age, sex, and operated lumbar levels in the PETLIF group, 100 patients (71 women and 29 men; mean age: 68.9 years) who underwent MIS-TLIF were randomly selected and included in the MIS-TLIF group. This retrospective investigation included surgical data, radiographic assessment, and clinical outcomes. RESULTS: The fusion rate was 95.1% and 96.0% in the PETLIF and MIS-TLIF groups, respectively (P=0.38). The decrease in hemoglobin levels from before surgery to 1 day after surgery was significantly lower in the PETLIF group than in the MIS-TLIF group (P<0.01). Five patients had detectable transient neurologic disorders after PETLIF that were resolved within 3 months. The increase in the local lordosis angle from before surgery to the final follow-up was significantly higher in the MIS-TLIF group than in the PETLIF group (P<0.01). Clinical scores were comparable between the two groups. CONCLUSIONS: Compared with MIS-TLIF, PETLIF showed excellent bone fusion rate and clinical outcomes. It was minimally invasive, resulting in less blood loss. However, exiting nerve root injury was a PETLIF-specific complication, and proper preventive management, including techniques to enlarge the Kambin's triangle, is required.
• Risk Factors for Delayed Diagnosis of Pyogenic Spondylitis: A Cross-Sectional Study with Prospective Case Series.
  Tomoya Sato; Katsuhisa Yamada; Keigo Yasui; Junichiro Okumura; Masahiro Kanayama; Ryota Hyakkan; Hiroyuki Hasebe; Yuichi Hasegawa; Hiroshi Nakayama; Tsutomu Endo; Daisuke Ukeba; Hiroyuki Tachi; Toshiya Chubachi; Hideki Sudo; Masahiko Takahata; Manabu Ito; Norimasa Iwasaki
  Spine surgery and related research, 9, 4, 485, 491, 27 Jul. 2025, [Domestic magazines]
  English, Scientific journal, INTRODUCTION: Delayed diagnosis and therapy initiation for pyogenic spondylitis can have severe and fatal consequences. Early diagnosis and intervention are crucial in the treatment of pyogenic spondylitis. This multicenter cross-sectional study with prospective case series aimed to identify factors influencing the time from symptom onset to the diagnosis of pyogenic spondylitis. METHODS: Patients hospitalized with pyogenic spondylitis between 2019 and 2023 were included. Patients were classified into 2 groups: the delayed diagnosis group (>30 days from the onset of initial symptoms to the diagnosis of pyogenic spondylitis) and the early diagnosis group (within 29 days). Risk factors for delayed diagnosis were analyzed. RESULTS: A total of 74 patients (42 men and 32 women; mean age: 70.2 years) from 5 institutions were included. Univariate analysis of risk factors for delayed diagnosis revealed that the significant risk factors included advanced age (p=0.03), low white blood cell count (p<0.01), low C-reactive protein level (p<0.05), and semi-rigid spinal level, based on the spinal instability neoplastic score classification (p=0.05). Multivariate analysis for delayed diagnosis showed that the location at the semi-rigid spinal level was a significant risk factor (p=0.02). The vertebral bone destruction rate and abscess cavity index in the delayed diagnosis group were significantly higher than those in the early diagnosis group (p<0.01 and p<0.01, respectively). CONCLUSIONS: Significant risk factors for delayed diagnosis of pyogenic spondylodiscitis include infection at the semi-rigid thoracic spinal level. Early diagnosis of spondylodiscitis is crucial because delayed diagnosis can lead to progressive bone destruction and the formation of large abscesses. Increased awareness of thoracic spinal infections, which can easily delay diagnosis, could help in the early diagnosis and treatment of pyogenic spondylodiscitis.
• The Potential Role of the Posterior Elements in Lumbar Spine Laminoplasty to Protect the Intervertebral Disc and Improve Walking Ability—Retrospective Comparative Study
  Namito Nakashita; Takashi Ohnishi; Tomomichi Kajino; Yuichiro Hisada; Hideki Sudo; Katsuhisa Yamada; Tsutomu Endo; Daisuke Ukeba; Yuichi Hasegawa; Toshiya Chubachi; Norimasa Iwasaki
  Journal of Clinical Medicine, 14, 12, 4014, 4014, MDPI AG, 06 Jun. 2025
  Scientific journal, Objectives: To investigate whether preservation of the posterior elements protects the spine from degeneration and improves postoperative symptoms in lumbar spine laminoplasty. Methods: Eighty-five consecutive patients who underwent lumbar spine laminoplasty were retrospectively reviewed. They were non-randomly stratified into two groups, the posterior elements resection (R) group and the preservation (P) group, and they were followed for two years after surgery. We radiographically analyzed the conditions of the spine and intervertebral disc (IVD) two years after surgery. The Japanese Orthopaedic Association Back Pain Evaluation Questionnaire (JOABPEQ) was used for symptom assessments. Logistic regression analysis was performed to determine whether the kissing spine was a significant factor for the outcomes in group R. Results: The 2-year D score increment and 2-year IVD height decrement was lower in group P. No difference was found in the flexion–extension angles or incidence of instability between groups. The JOABPEQ revealed higher scores in walking ability, social life function, and mental health in group P one year after surgery. Walking ability was the only score that remained higher two years after surgery. The visual analog scale of pain in the buttocks and lower limbs was lower in group P only one year after surgery. Finally, the kissing spine was not a significant factor in any outcome. Conclusions: The preserved posterior elements were considered to protect the IVD in lumbar spine laminoplasty. In addition, they positively affected postoperative health status from multiple aspects.
• Acellular, bioresorbable, ultra-purified alginate gel implantation for intervertebral disc herniation: Phase 1/2, open-label, non-randomized clinical trials
  Katsuhisa Yamada; Takahiko Hyakumachi; Terufumi Kokabu; Kenichiro Maeda; Toshiyuki Isoe; Khin Khin Tha; Yoichi M. Ito; Takashi Ohnishi; Tsutomu Endo; Daisuke Ukeba; Hiroyuki Tachi; Yuichiro Abe; Yoko Ishikawa; Nozomi Yokota; Takashi Miyakoshi; Osamu Sugita; Norihiro Sato; Norimasa Iwasaki; Hideki Sudo
  Nature Communications, 16, 1, Springer Science and Business Media LLC, 08 May 2025, [Peer-reviewed], [Last author, Corresponding author]
  Scientific journal
• Artificial intelligence-driven 3D MRI of lumbosacral nerve root anomalies: accuracy, incidence, and clinical utility.
  Daisuke Ukeba; Ken Nagahama; Katsuhisa Yamada; Yuichiro Abe; Yoshinori Hyugaji; Tsutomu Endo; Takashi Ohnishi; Hiroyuki Tachi; Yuichi Hasegawa; Hideki Sudo; Norimasa Iwasaki
  Neuroradiology, 01 Mar. 2025, [Peer-reviewed], [International Magazine]
  English, Scientific journal, PURPOSE: Lumbosacral nerve root anomalies are relatively rare but can be a risk factor for intraoperative nerve injury. However, it is often difficult to evaluate them with preoperative imaging. We developed a software that automatically generates three-dimensional (3D) nerve root images from magnetic resonance (MR) imaging using artificial intelligence (AI). This study aims to evaluate the accuracy and utility of this modality in clinical practice by conducting an epidemiological study of nerve root anomalies. METHODS: The incidence and morphology of nerve root anomalies were evaluated in the 3D images of 1,500 patients. The accuracy of the images was evaluated by comparing the images generated automatically using this AI software with those created manually by conventional methods. RESULTS: Of 1,500 cases, 53 (3.5%) had nerve root anomalies with total of 58 nerve root anomalies. With respect to the spinal level, 35 nerve root anomalies were found in the L5-S1 level, the most common (60.3%). As for morphology, 47 nerve roots (81.0%) were of the Neidre-MacNab classification Type 1. The images matched in 1,493 out of 1,500 cases (99.5%) between the two methods, and the remaining 7 cases all had nerve root abnormalities, which were detected as abnormal by the AI software. CONCLUSION: The MR nerve root 3D imaging provided a 3D visualization and understanding of nerve root morphology, including nerve root anomalies. The AI software enables easy and precise 3D nerve root imaging, which greatly aids in the preoperative evaluation for spinal surgery.
• Four-dimensional anatomical spinal reconstruction using pre-bent rods in thoracic adolescent idiopathic scoliosis.
  Akira Fukushima; Takashi Ohnishi; Terufumi Kokabu; Yuichiro Abe; Hiroyuki Tachi; Tsutomu Endo; Daisuke Ukeba; Norimasa Iwasaki; Katsuhisa Yamada; Hideki Sudo
  Scientific reports, 15, 1, 378, 378, 02 Jan. 2025, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, A four-dimensional (4D) anatomical spinal reconstruction (ASR) technique and anatomical notch-free, pre-bent rods have been developed for thoracic adolescent idiopathic scoliosis (AIS) surgery. We aimed to evaluate the outcomes of ASR using notch-free rods through multiple comparisons with conventional methods, including the simultaneous double-rod rotation technique (SDRRT) and ASR using manually bent notched rods. Three consecutive series of 126 patients who underwent surgery for Lenke 1 AIS curves were prospectively followed up for 2 years after surgery. The operative time was significantly shorter in the ASR using notch-free rods group than in the other two groups (P < 0.05). The correction rate of the main thoracic (MT) curve was higher in the ASR group than in the SDRRT group (P < 0.01). Thoracic kyphosis (TK) was greater in the ASR using notch-free rods group than in the other two groups at the final follow-up (P < 0.01). The percentage of patients with a T6-T8 location of the TK apex was greater in the ASR using notch-free rods group than in the SDRRT group at the final follow-up (P < 0.01). ASR using notch-free rods created an anatomical TK, contributing to a shorter operative time and standardization of the procedure.
• Visceral Fat and Ossification of the Posterior Longitudinal Ligament: Insights From a Japanese Cohort.
  Tomoya Sato; Tsutomu Endo; Yoshinao Koike; Hideki Sudo; M Alaa Terkawi; Huohuo Xue; Ryo Fujita; Soya Miura; Ryota Suzuki; Yukitoshi Shimamura; Masahiro Kanayama; Ken Kadoya; Katsuhisa Yamada; Daisuke Ukeba; Misaki Ishii; Norimasa Iwasaki
  JB & JS open access, 10, 4, 2025, [International Magazine]
  English, Scientific journal, BACKGROUND: Ossification of the posterior longitudinal ligament (OPLL) is relatively common in East Asian populations, with a recently revealed link to obesity. However, evidence linking OPLL with visceral fat obesity, which is prevalent in the Asian population, is insufficient. We aimed to examine the association between visceral fat obesity and the development of OPLL. METHODS: In a single-center case-control study, data were collected from 120 Japanese patients diagnosed with OPLL and 91 controls without spinal ligament ossification identified during health screenings. From 2020 to 2023, all participants underwent computed tomography to assess visceral fat content and spinal ligament ossification. OPLL was classified as localized (cervical spine) or diffuse (thoracic/lumbar spine). Multivariable logistic regression was conducted to assess the effect size (odds ratio [OR]) of body mass index on the incidence of OPLL and to compare outcomes between groups with a high and low visceral/subcutaneous fat area (V/S) ratio. RESULTS: The proportion of patients with visceral fat obesity was significantly higher in both the localized and diffuse OPLL groups than in the controls (58.9% vs. 64.1% vs. 25.2%, p < 0.05). Patients with OPLL had a higher rate of comorbid visceral fat obesity than the propensity score-matched controls did (56.8% vs. 18.1%, p < 0.001). The effect of BMI on the development of diffuse OPLL was 2.6-fold greater in the high V/S ratio group (OR, 9.50; 95% confidence interval [CI], 2.11 to 42.71) than in the low V/S ratio group (OR, 3.56; 95% CI, 1.51-8.37). CONCLUSIONS: Visceral fat obesity was associated with the development of OPLL, particularly diffuse OPLL. The modifying effect of visceral fat accumulation with overweight status was more strongly associated with diffuse OPLL than was that of subcutaneous fat combined with an overweight status. LEVEL OF EVIDENCE: Therapeutic Level III. See Instructions for Authors for a complete description of levels of evidence.
• Duckbill Release Technique for the Outside-in Method in Full-Endoscopic Spine Surgery via Transforaminal Approach: A Technical Note.
  Daisuke Ukeba; Ken Nagahama; Katsuhisa Yamada; Yuichiro Abe; Yoshinori Hyugaji; Yuto Horita; Tsutomu Endo; Takashi Ohnishi; Hiroyuki Tachi; Yuichi Hasegawa; Hideki Sudo; Norimasa Iwasaki
  World neurosurgery, 193, 149, 154, Jan. 2025, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: In full-endoscopic spine surgery via transforaminal approach (FESS-TF), the outside-in method facilitates treatment for various cases; however, the technique is difficult to perform. To facilitate this procedure, we developed a duckbill release technique. In this article, we aimed to introduce the details of this technique and investigate its effectiveness. METHODS: Fifty patients who underwent FESS-TF for intervertebral disc herniation were divided into 2 groups: the conventional technique and the duckbill release technique groups. The time from the initiation of surgery to exposure of the bone outside the superior articular process was measured in both groups. The visual analog scale score for leg pain and the Japanese Orthopaedic Association score before and after surgery were compared in terms of clinical outcomes. RESULTS: No significant differences were observed between the 2 groups in terms of patient background. The approach time was 252 ± 86 seconds in the conventional group, while it was 105 ± 26 seconds in the duckbill release group, which was significantly shorter (P < 0.001). In terms of clinical outcomes, the 2 groups did not significantly differ in all endpoints of the visual analog scale score for leg pain or the Japanese Orthopaedic Association score. CONCLUSIONS: The duckbill release technique for the outside-in method in FESS-TF could be efficient because it is a simple and time-saving approach.
• Visceral fat obesity predicts ossification of the posterior longitudinal ligament: annual health examination data-based evidence
  Soya Miura; Yoshinao Koike; Tsutomu Endo; Masahiko Takahata; Hideki Sudo; Ken Kadoya; Masahiro Kanayama; Ryo Fujita; Shotaro Fukada; M Alaa Terkawi; Katsuhisa Yamada; Takashi Ohnishi; Daisuke Ukeba; Hiroyuki Tachi; Yuichi Hasegawa; Misaki Ishii; Norimasa Iwasaki
  The Spine Journal, Elsevier BV, Jan. 2025, [Peer-reviewed]
  Scientific journal
• Posterior Correction and Fusion Using a 4D Anatomical Spinal Reconstruction Technique Improves Postural Stability Under the Eye-Closed Condition in Patients with Adolescent Idiopathic Scoliosis.
  Satoshi Osuka; Hideki Sudo; Katsuhisa Yamada; Hiroyuki Tachi; Akira Fukushima; Hiroki Mani; Kentaro Watanabe; Fuma Sentoku; Takeshi Chiba; Hiroaki Hori; Norimasa Iwasaki; Masahiko Mukaino; Harukazu Tohyama
  Journal of clinical medicine, 13, 21, 24 Oct. 2024, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Background: Patients with adolescent idiopathic scoliosis (AIS) has been reported to exhibit impaired postural stability. Posterior correction and fusion using four-dimensional (4D) anatomical spinal reconstruction techniques may improve postural stability to correct the spine for optimal anatomical alignment. This prospective study aimed to determine the effect of posterior correction and fusion using a 4D anatomical spinal reconstruction technique on postural stability in the eye-open and eye-closed standing position in patients with thoracic AIS. Methods: Thirty-three patients with AIS, excluding those with Lenke type 5C AIS, participated in the study. The mean and standard deviation of the minimum values of the time-to-boundary (TTB) were determined. All patients were asked to perform the quiet standing position under the eye-open and eye-closed condition on a force plate preoperatively and at 1 week and 2 years postoperatively. The TTB value was calculated from the velocity and distance to the foot boundary of the acquired center-of-pressure data. Results: Under the eye-closed condition, the mean and standard deviation of the minimum TTB were significantly higher at 2 years postoperatively than preoperatively and at 1 week postoperatively. The mean and standard deviation of the minimum TTB values were significantly lower at 1 week postoperatively than preoperatively. Conclusions: The results of this study suggest that surgery using the 4D anatomical spinal reconstruction technique reduces postural stability immediately after surgery; however, it improves postural stability at 2 years compared to the preoperative values.
• 敵対的生成ネットワークによる単純CTからAIミエロCTの生成
  小甲 晃史; 山田 勝久; 糸賀 稜; 鈴木 久崇; 藪 晋人; 石川 蓉子; 柳橋 寧; 百町 貴彦; 清水 智弘; 須藤 英毅; 岩崎 倫政
  日本整形外科学会雑誌, 98, 8, S1826, S1826, (公社)日本整形外科学会, Sep. 2024
  Japanese
• 敵対的生成ネットワークによる単純CTからAIミエロCTの生成
  小甲 晃史; 山田 勝久; 糸賀 稜; 鈴木 久崇; 柳橋 寧; 百町 貴彦; 清水 智弘; 須藤 英毅; 岩崎 倫政
  東日本整形災害外科学会雑誌, 36, 3, 275, 275, 東日本整形災害外科学会, Aug. 2024
  Japanese
• Reverse Translational Approach Using Biomaterials and Stem Cells for Intervertebral Disc Degeneration.
  Katsuhisa Yamada; Hideki Sudo; Norimasa Iwasaki
  JMA journal, 7, 3, 423, 425, 16 Jul. 2024, [Domestic magazines]
  English, Scientific journal
• Deep learning-based detection of lumbar spinal canal stenosis using convolutional neural networks.
  Hisataka Suzuki; Terufumi Kokabu; Katsuhisa Yamada; Yoko Ishikawa; Akito Yabu; Yasushi Yanagihashi; Takahiko Hyakumachi; Hiroyuki Tachi; Tomohiro Shimizu; Tsutomu Endo; Takashi Ohnishi; Daisuke Ukeba; Ken Nagahama; Masahiko Takahata; Hideki Sudo; Norimasa Iwasaki
  The spine journal : official journal of the North American Spine Society, 22 Jun. 2024, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND CONTEXT: Lumbar spinal canal stenosis (LSCS) is the most common spinal degenerative disorder in elderly people and usually first seen by primary care physicians or orthopedic surgeons who are not spine surgery specialists. Magnetic resonance imaging (MRI) is useful in the diagnosis of LSCS, but the equipment is often not available or difficult to read. LSCS patients with progressive neurologic deficits have difficulty with recovery if surgical treatment is delayed. So, early diagnosis and determination of appropriate surgical indications are crucial in the treatment of LSCS. Convolutional neural networks (CNNs), a type of deep learning, offers significant advantages for image recognition and classification, and work well with radiographs, which can be easily taken at any facility. PURPOSE: Our purpose was to develop an algorithm to diagnose the presence or absence of LSCS requiring surgery from plain radiographs using CNNs. STUDY DESIGN: Retrospective analysis of consecutive, nonrandomized series of patients at a single institution. PATIENT SAMPLE: Data of 150 patients who underwent surgery for LSCS, including degenerative spondylolisthesis, at a single institution from January 2022 to August 2022, were collected. Additionally, 25 patients who underwent surgery at 2 other hospitals were included for extra external validation. OUTCOME MEASURES: In annotation 1, the area under the curve (AUC) computed from the receiver operating characteristic (ROC) curve, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), accuracy, positive likelihood ratio (PLR), and negative likelihood ratio (NLR) were calculated. In annotation 2, correlation coefficients were used. METHODS: Four intervertebral levels from L1/2 to L4/5 were extracted as region of interest from lateral plain lumbar spine radiographs totaling 600 images were obtained. Based on the date of surgery, 500 images derived from the first 125 cases were used for internal validation, and 100 images from the subsequent 25 cases used for external validation. Additionally, 100 images from other hospitals were used for extra external validation. In annotation 1, binary classification of operative and nonoperative levels was used, and in annotation 2, the spinal canal area measured on axial MRI was labeled as the output layer. For internal validation, the 500 images were divided into each 5 dataset on per-patient basis and 5-fold cross-validation was performed. Five trained models were registered in the external validation prediction performance. Grad-CAM was used to visualize area with the high features extracted by CNNs. RESULTS: In internal validation, the AUC and accuracy for annotation 1 ranged between 0.85-0.89 and 79-83%, respectively, and the correlation coefficients for annotation 2 ranged between 0.53 and 0.64 (all p<.01). In external validation, the AUC and accuracy for annotation 1 were 0.90 and 82%, respectively, and the correlation coefficient for annotation 2 was 0.69, using 5 trained CNN models. In the extra external validation, the AUC and accuracy for annotation 1 were 0.89 and 84%, respectively, and the correlation coefficient for annotation 2 was 0.56. Grad-CAM showed high feature density in the intervertebral joints and posterior intervertebral discs. CONCLUSIONS: This technology automatically detects LSCS from plain lumbar spine radiographs, making it possible for medical facilities without MRI or nonspecialists to diagnose LSCS, suggesting the possibility of eliminating delays in the diagnosis and treatment of LSCS that require early treatment.
• Ossification of the posterior longitudinal ligament is linked to heterotopic ossification of the ankle/foot tendons.
  Tsutomu Endo; Masahiko Takahata; Yoshinao Koike; Ryo Fujita; Daisuke Yoneoka; Masahiro Kanayama; Ken Kadoya; Tomoka Hasegawa; Mohamad Alaa Terkawi; Katsuhisa Yamada; Hideki Sudo; Taku Ebata; Misaki Ishii; Norimasa Iwasaki
  Journal of bone and mineral metabolism, 08 Jun. 2024, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, INTRODUCTION: Systemic osteogenesis has been speculated to be involved in the pathogenesis of ossification of the posterior longitudinal ligament (OPLL). Our purpose was to compare the radiologic prevalence and severity of heterotopic ossification in foot tendons of Japanese patients with OPLL and to determine their association with systemic heterotopic ossification. MATERIALS AND METHODS: Clinical and radiographic data of 114 patients with OPLL were collected from 2020 to 2022. Control data were extracted from a medical database of 362 patients with ankle radiographs. Achilles and plantar tendon ossification were classified as grades 0-4, and the presence of osteophytes at five sites in the foot/ankle joint was assessed by radiography. Factors associated with the presence and severity of each ossification were evaluated by multivariable logistic regression and linear regression analysis. RESULTS: The prevalence of Achilles and plantar tendon ossification (grade ≥ 2) was 4.0-5.5 times higher in patients with OPLL (40-56%) than in the controls (10-11%). The presence of Achilles tendon ossification was associated with OPLL, age, and coexisting plantar tendon ossification, and was most strongly associated with OPLL (standardized regression coefficient, 0.79; 95% confidence interval, 1.34-2.38). The severity of Achilles and plantar tendon ossification was associated with the severity of ossification of the entire spinal ligament. CONCLUSIONS: The strong association of foot tendon ossification with OPLL suggests that patients with OPLL have a systemic osteogenesis background. These findings will provide a basis for exploring new treatment strategies for OPLL, including control of metabolic abnormalities.
• Bone Marrow Aspirate Concentrate Combined with Ultra-Purified Alginate Bioresorbable Gel Enhances Intervertebral Disc Repair in a Canine Model: A Preclinical Proof-of-Concept Study.
  Daisuke Ukeba; Yoko Ishikawa; Katsuhisa Yamada; Takashi Ohnishi; Hiroyuki Tachi; Khin Khin Tha; Norimasa Iwasaki; Hideki Sudo
  Cells, 13, 11, 05 Jun. 2024, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Although discectomy is commonly performed for lumbar intervertebral disc (IVD) herniation, the capacity for tissue repair after surgery is limited, resulting in residual lower back pain, recurrence of IVD herniation, and progression of IVD degeneration. Cell-based therapies, as one-step procedures, are desirable for enhancing IVD repair. This study aimed to investigate the therapeutic efficacy of a combination of newly developed ultra-purified alginate (UPAL) gel and bone marrow aspirate concentrate (BMAC) implantation for IVD repair after discectomy. Prior to an in vivo study, the cell concentration abilities of three commercially available preparation kits for creating the BMAC were compared by measuring the number of bone marrow mesenchymal stem cells harvested from the bone marrow of rabbits. Subsequently, canine-derived BMAC was tested in a canine model using a kit which had the highest concentration rate. At 24 weeks after implantation, we evaluated the changes in the magnetic resonance imaging (MRI) signals as well as histological degeneration grade and immunohistochemical analysis results for type II and type I collagen-positive cells in the treated IVDs. In all quantitative evaluations, such as MRI and histological and immunohistochemical analyses of IVD degeneration, BMAC-UPAL implantation significantly suppressed the progression of IVD degeneration compared to discectomy and UPAL alone. This preclinical proof-of-concept study demonstrated the potential efficacy of BMAC-UPAL gel as a therapeutic strategy for implementation after discectomy, which was superior to UPAL and discectomy alone in terms of tissue repair and regenerative potential.
• 転移性脊椎腫瘍に対する手術治療における立位歩行獲得と合併症発生予測の必要性について
  岩田 玲; 高畑 雅彦; 須藤 英毅; 山田 勝久; 松岡 正剛; 伊東 学; 安井 啓悟; 奥村 潤一郎; 楫野 知道; 放生 憲博; 平塚 重人; 原谷 健太郎; 清水 寛和; 相馬 有; 岩崎 倫政; 平賀 博明
  日本整形外科学会雑誌, 98, 6, S1636, S1636, (公社)日本整形外科学会, Jun. 2024
  Japanese
• 放射線治療後の姑息的転移性脊椎腫瘍手術に対する検討
  岩田 玲; 高畑 雅彦; 安井 啓悟; 伊東 学; 楫野 知道; 奥村 潤一郎; 放生 憲博; 平塚 重人; 原谷 健太郎; 須藤 英毅; 松岡 正剛; 山田 勝久; 清水 寛和; 岩崎 倫政; 平賀 博明
  Journal of Spine Research, 15, 3, 329, 329, (一社)日本脊椎脊髄病学会, Feb. 2024
  Japanese
• Intervertebral Disc Degeneration and Regeneration: New Molecular Mechanisms and Therapeutics
  Hideki Sudo
  Cells, 13, 2, 153, 153, MDPI AG, 15 Jan. 2024, [Peer-reviewed], [Invited], [Lead author, Last author, Corresponding author]
  Scientific journal, The intervertebral disc (IVD) is a soft tissue that constitutes the spinal column together with the vertebrae, and consists of the central nucleus pulposus (gelatinous tissue) and the annulus fibrosus (rich in fibrous tissue) that surrounds the nucleus pulposus [...]
• Development of Notch-Free, Pre-Bent Rod Applicable for Posterior Corrective Surgery of Thoracolumbar/Lumbar Adolescent Idiopathic Scoliosis.
  Yoko Ishikawa; Satoshi Kanai; Katsuro Ura; Terufumi Kokabu; Katsuhisa Yamada; Yuichiro Abe; Hiroyuki Tachi; Hisataka Suzuki; Takashi Ohnishi; Tsutomu Endo; Daisuke Ukeba; Masahiko Takahata; Norimasa Iwasaki; Hideki Sudo
  Journal of clinical medicine, 12, 17, 04 Sep. 2023, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Adolescent idiopathic scoliosis (AIS), the most common pediatric musculoskeletal disorder, causes a three-dimensional spine deformity. Lenke type 5 AIS is defined as a structural thoracolumbar/lumbar curve with nonstructural thoracic curves. Although a rod curvature will affect clinical outcomes, intraoperative contouring of the straight rod depends on the surgeon's knowledge and experience. This study aimed to determine the optimum rod geometries to provide a pre-bent rod system for posterior spinal surgery in patients with Lenke type 5 AIS. These pre-bent rods will be beneficial for achieving proper postoperative outcomes without rod contouring based on surgeon experience. We investigated 20 rod geometries traced in posterior spinal reconstruction in patients with Lenke type 5 AIS. The differences between the center point clouds in each cluster were evaluated using the iterative closest point (ICP) method with modification. Before the evaluation using the ICP method, the point clouds were divided into four clusters based on the rod length using a hierarchical cluster analysis. Because the differences in the values derived from the ICP method were <5 mm for each length-based cluster, four representative rod shapes were generated from the length-based clusters. We identified four optimized rod shapes that will reduce operation time, leading to a decreased patient and surgeon burden.
• A Review: Methodologies to Promote the Differentiation of Mesenchymal Stem Cells for the Regeneration of Intervertebral Disc Cells Following Intervertebral Disc Degeneration.
  Takashi Ohnishi; Kentaro Homan; Akira Fukushima; Daisuke Ukeba; Norimasa Iwasaki; Hideki Sudo
  Cells, 12, 17, 28 Aug. 2023, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Intervertebral disc (IVD) degeneration (IDD), a highly prevalent pathological condition worldwide, is widely associated with back pain. Treatments available compensate for the impaired function of the degenerated IVD but typically have incomplete resolutions because of their adverse complications. Therefore, fundamental regenerative treatments need exploration. Mesenchymal stem cell (MSC) therapy has been recognized as a mainstream research objective by the World Health Organization and was consequently studied by various research groups. Implanted MSCs exert anti-inflammatory, anti-apoptotic, and anti-pyroptotic effects and promote extracellular component production, as well as differentiation into IVD cells themselves. Hence, the ultimate goal of MSC therapy is to recover IVD cells and consequently regenerate the extracellular matrix of degenerated IVDs. Notably, in addition to MSC implantation, healthy nucleus pulposus (NP) cells (NPCs) have been implanted to regenerate NP, which is currently undergoing clinical trials. NPC-derived exosomes have been investigated for their ability to differentiate MSCs from NPC-like phenotypes. A stable and economical source of IVD cells may include allogeneic MSCs from the cell bank for differentiation into IVD cells. Therefore, multiple alternative therapeutic options should be considered if a refined protocol for the differentiation of MSCs into IVD cells is established. In this study, we comprehensively reviewed the molecules, scaffolds, and environmental factors that facilitate the differentiation of MSCs into IVD cells for regenerative therapies for IDD.
• High whole-body bone mineral density in ossification of the posterior longitudinal ligament.
  Ryo Fujita; Tsutomu Endo; Masahiko Takahata; Yoshinao Koike; Daisuke Yoneoka; Ryota Suzuki; Masaru Tanaka; Katsuhisa Yamada; Hideki Sudo; Tomoka Hasegawa; Mohamad Alaa Terkawi; Ken Kadoya; Norimasa Iwasaki
  The spine journal : official journal of the North American Spine Society, 23, 10, 1461, 1470, 10 Jul. 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND CONTEXT: Recent studies suggest that ossification of the posterior longitudinal ligament (OPLL) is exacerbated by systemic metabolic disturbances, including obesity. However, although an increase in bone mineral density (BMD) measured at the lumbar spine has been reported in patients with OPLL, no studies have investigated the systemic BMD of patients with OPLL in detail. PURPOSE: We investigated whether patients with OPLL develop increased whole-body BMD. STUDY DESIGN: Single institution cross-sectional study. PATIENT SAMPLE: Data were collected from Japanese patients with symptomatic OPLL (OPLL [+]; n=99). Control data (OPLL [-]; n=226) without spinal ligament ossification were collected from patients who underwent spinal decompression, spinal fusion, or hip replacement surgery. OUTCOME MEASURES: Demographic data, including age, body mass index (BMI), comorbidities, history of treatment for osteoporosis, and history of vertebral and nonvertebral fractures, was obtained from all participants. In addition, whole-body BMD, including the lumbar spine, thoracic spine, femoral neck, skull, ribs, entire upper extremity, entire lower extremity, and pelvis, were measured in all participants using whole-body dual-energy X-ray absorptiometry. METHODS: Patient data were collected from 2018 to 2022. All participants were categorized based on sex, age (middle-aged [<70 years] and older adults [≥70 years]), and OPLL type (localized OPLL [OPLL only in the cervical spine], diffuse OPLL [OPLL in regions including the thoracic spine]), and OPLL [-]) and each parameter was compared. The factors associated with whole-body BMD were evaluated via multivariable linear regression analysis. RESULTS: Compared with the OPLL (-) group, the OPLL (+) group of older women had significantly higher BMD in all body parts (p<.01), and the OPLL (+) group of older men had significantly higher BMD in all body parts except the ribs, forearm, and skull (p<.01). The factors associated with increased BMD of both the femoral neck (load-bearing bone) and skull (nonload-bearing bone) were age, BMI, and coexisting diffuse OPLL in women and BMI and coexisting localized OPLL in men. CONCLUSIONS: Patients with OPLL have increased whole-body BMD regardless of sex, indicating that it is not simply due to load-bearing from obesity. These findings suggested that OPLL is associated with a systemic pathology.
• Factors Affecting Transcranial Motor-Evoked Potential Measurements Using Single-Train Stimulation with an Increased Number of Pulses during Adolescent Scoliosis Surgery: A Prospective Observational Study.
  Takayuki Toki; Noriaki Fujita; Tomohiro Ichikawa; Noriki Ochi; Isao Yokota; Hideki Sudo; Yuji Morimoto
  Journal of clinical medicine, 12, 13, 30 Jun. 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Measurement of transcranial motor-evoked potentials (TcMEPs) during scoliosis surgery helps detect postoperative new neurological defects. However, TcMEP interpretation is difficult owing to the influence of intraoperative physiological, pharmacological, and time-related factors as well as stimulation conditions. In this study, we aimed to investigate the effect of the abovementioned factors on TcMEP amplitude using single-train stimulation with an increased number of pulses (STS-INP) during adolescent scoliosis surgery; moreover, we evaluated the complications of TcMEP measurement. We included 50 patients and 706 TcMEP measurements. A total of 1412 TcMEP waveforms were analyzed, each on the bilateral abductor pollicis brevis, tibialis anterior, and abductor hallucis muscles. We estimated the mean difference (95% confidence interval (CI)) and predicted mean difference (95% CI) evaluated using the interquartile range of each factor, based on a mixed-effect model with random intercepts for TcMEP amplitude. The predicted mean differences in TcMEP amplitude were clinically small compared with the actual TcMEP amplitude, suggesting that each factor had a limited effect on TcMEP amplitude. No intraoperative bite injuries or seizures were observed. Using STS-INP during adolescent scoliosis surgery may enable accurate measurement of TcMEP amplitude with neither complications nor the influence of various intraoperative factors.
• Quantitative Assessment of Intervertebral Disc Composition by MRI: Sensitivity to Diurnal Variation.
  Hiroyuki Hamaguchi; Maho Kitagawa; Daiki Sakamoto; Ulrich Katscher; Hideki Sudo; Katsuhisa Yamada; Kohsuke Kudo; Khin Khin Tha
  Tomography (Ann Arbor, Mich.), 9, 3, 1029, 1040, 16 May 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Whether diurnal variation exists in quantitative MRI indices such as the T1rho relaxation time (T1ρ) of the intervertebral disc (IVD) is yet to be explored. This prospective study aimed to evaluate the diurnal variation in T1ρ, apparent diffusion coefficient (ADC), and electrical conductivity (σ) of lumbar IVD and its relationship with other MRI or clinical indices. Lumbar spine MRI, including T1ρ imaging, diffusion-weighted imaging (DWI), and electric properties tomography (EPT), was conducted on 17 sedentary workers twice (morning and evening) on the same day. The T1ρ, ADC, and σ of IVD were compared between the time points. Their diurnal variation, if any, was tested for correlation with age, body mass index (BMI), IVD level, Pfirrmann grade, scan interval, and diurnal variation in IVD height index. The results showed a significant decrease in T1ρ and ADC and a significant increase in the σ of IVD in the evening. T1ρ variation had a weak correlation with age and scan interval, and ADC variation with scan interval. Diurnal variation exists for the T1ρ, ADC, and σ of lumbar IVD, which should be accounted for in image interpretation. This variation is thought to be due to diurnal variations in intradiscal water, proteoglycan, and sodium ion concentration.
• Dyslipidemia as a novel risk for the development of symptomatic ossification of the posterior longitudinal ligament.
  Shotaro Fukada; Tsutomu Endo; Masahiko Takahata; Masahiro Kanayama; Yoshinao Koike; Ryo Fujita; Ryota Suzuki; Toshifumi Murakami; Tomoka Hasegawa; Mohamad Alaa Terkawi; Tomoyuki Hashimoto; Kastuhisa Yamada; Hideki Sudo; Ken Kadoya; Norimasa Iwasaki
  The spine journal : official journal of the North American Spine Society, 23, 9, 1287, 1295, 07 May 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND CONTEXT: Obesity and visceral fat have been implicated as potential factors in the pathogenesis of the ossification of the posterior longitudinal ligament (OPLL); the details of the factors involved in OPLL remain unclear. PURPOSE: We aimed to determine the association between dyslipidemia and symptomatic OPLL. STUDY DESIGN: Single institution cross-sectional study. PATIENT SAMPLE: Data were collected from Japanese patients with OPLL (n=92) who underwent whole-spine computed tomography scanning. Control data (n=246) without any spinal ligament ossification were collected from 627 Japanese participants who underwent physical examination. OUTCOME MEASURES: Baseline information and lipid parameters, including triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) from fasting blood samples were collected to assess the comorbidity of dyslipidemia. METHODS: Patient data were collected from 2020 to 2022. Patients with dyslipidemia were defined as those who were taking medication for dyslipidemia and who met one of the following criteria: TG ≥150 mg/dL, LDL-C ≥140 mg/dL, and/or HDL-C <40 mg/dL. The factors associated with OPLL development were evaluated using multivariate logistic regression analysis. RESULTS: The comorbidity of dyslipidemia in the OPLL group was more than twice that in the control group (71.7% and 35.4%, respectively). The mean body mass index (BMI) of the OPLL group was significantly higher than that of the control group (27.2 kg/m2 and 23.0 kg/m2). Multivariate logistic regression analysis revealed that dyslipidemia was associated with the development of OPLL (regression coefficient, 0.80; 95% confidence interval, 0.11-1.50). Additional risk factors included age, BMI, and diabetes mellitus. CONCLUSIONS: We demonstrated a novel association between dyslipidemia and symptomatic OPLL development using serum data. This suggests that visceral fat obesity or abnormal lipid metabolism are associated with the mechanisms of onset and exacerbation of OPLL as well as focal mechanical irritation due to being overweight.
• Evaluation of Surgical Indications for Full Endoscopic Discectomy at Lumbosacral Disc Levels Using Three-Dimensional Magnetic Resonance/Computed Tomography Fusion Images Created with Artificial Intelligence.
  Katsuhisa Yamada; Ken Nagahama; Yuichiro Abe; Yoshinori Hyugaji; Daisuke Ukeba; Tsutomu Endo; Takashi Ohnishi; Katsuro Ura; Hideki Sudo; Norimasa Iwasaki; Masahiko Takahata
  Medicina (Kaunas, Lithuania), 59, 5, 28 Apr. 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Background and Objectives: Although full endoscopic lumbar discectomy with the transforaminal approach (FED-TF) is a minimally invasive spinal surgery for lumbar disc herniation, the lumbosacral levels present anatomical challenges when performing FED-TF surgery due to the presence of the iliac bone. Materials and Methods: In this study, we simulated whether FED-TF surgery could be safely performed on a total of 52 consecutive cases with L5-S1 or L5-L6 disc herniation using fused three-dimensional (3D) images of the lumbar nerve root on magnetic resonance imaging (MRI) created with artificial intelligence and of the lumbosacral spine and iliac on computed tomography (CT) images. Results: Thirteen of the fifty-two cases were deemed operable according to simulated FED-TF surgery without foraminoplasty using the 3D MRI/CT fusion images. All 13 cases underwent FED-TF surgery without neurological complications, and their clinical symptoms significantly improved. Conclusions: Three-dimensional simulation may allow for the assessment from multiple angles of the endoscope entry and path, as well as the insertion angle. FED-TF surgery simulation using 3D MRI/CT fusion images could be useful in determining the indications for full endoscopic surgery for lumbosacral disc herniation.
• Influence of Lateral Translation of Lowest Instrumented Vertebra on L4 Tilt and Coronal Balance for Thoracolumbar and Lumbar Curves in Adolescent Idiopathic Scoliosis
  Katsuhisa Yamada; Hideki Sudo; Yuichiro Abe; Terufumi Kokabu; Hiroyuki Tachi; Tsutomu Endo; Takashi Ohnishi; Daisuke Ukeba; Katsuro Ura; Masahiko Takahata; Norimasa Iwasaki
  Journal of Clinical Medicine, 12, 4, 1389, 1389, MDPI AG, 09 Feb. 2023, [Peer-reviewed], [Corresponding author]
  Scientific journal, This study aimed to evaluate the lowest instrumented vertebra translation (LIV-T) in the surgical treatment of thoracolumbar/lumbar adolescent idiopathic scoliosis and to analyze the radiographic parameters in relation to LIV-T and L4 tilt and global coronal balance. A total of 62 patients underwent posterior spinal fusion (PSF, n = 32) or anterior spinal fusion (ASF, n = 30) and were followed up for a minimum of 2 years. The mean preoperative LIV-T was significantly larger in the ASF group than the PSF (p < 0.01), while the final LIV-T was equivalent. LIV-T at the final follow-up was significantly correlated with L4 tilt and the global coronal balance (r = 0.69, p < 0.01, r = 0.38, p < 0.01, respectively). Receiver-operating characteristic analysis for good outcomes, with L4 tilt <8° and coronal balance <15 mm at the final follow-up, calculated the cutoff value of the final LIV-T as 12 mm. The cutoff value of preoperative LIV-T that would result in the LIV-T of ≤12 mm at the final follow-up was 32 mm in PSF, although no significant cutoff value was calculated in ASF. ASF can centralize the LIV better than PSF with a shorter segment fusion, and could be useful in obtaining a good curve correction and global balance without fixation to L4 in cases with large preoperative LIV-T.
• Injection of Ultra-Purified Stem Cells with Sodium Alginate Reduces Discogenic Pain in a Rat Model
  Hisataka Suzuki; Katsuro Ura; Daisuke Ukeba; Takashi Suyama; Norimasa Iwasaki; Masatoki Watanabe; Yumi Matsuzaki; Katsuhisa Yamada; Hideki Sudo
  Cells, 12, 3, 505, 505, MDPI AG, 03 Feb. 2023, [Peer-reviewed], [Last author, Corresponding author]
  Scientific journal, Intervertebral disc (IVD) degeneration is a major cause of low back pain. However, treatments directly approaching the etiology of IVD degeneration and discogenic pain are not yet established. We previously demonstrated that intradiscal implantation of cell-free bioresorbable ultra-purified alginate (UPAL) gel promotes tissue repair and reduces discogenic pain, and a combination of ultra-purified, Good Manufacturing Practice (GMP)-compliant, human bone marrow mesenchymal stem cells (rapidly expanding clones; RECs), and the UPAL gel increasingly enhanced IVD regeneration in animal models. This study investigated the therapeutic efficacy of injecting a mixture of REC and UPAL non-gelling solution for discogenic pain and IVD regeneration in a rat caudal nucleus pulposus punch model. REC and UPAL mixture and UPAL alone suppressed not only the expression of TNF-α, IL-6, and TrkA (p < 0.01, respectively), but also IVD degeneration and nociceptive behavior compared to punching alone (p < 0.01, respectively). Furthermore, REC and UPAL mixture suppressed these expression levels and nociceptive behavior compared to UPAL alone (p < 0.01, respectively). These results suggest that this minimally invasive treatment strategy with a single injection may be applied to treat discogenic pain and as a regenerative therapy.
• Protocol for treating lumbar spinal canal stenosis with a combination of ultrapurified, allogenic bone marrow-derived mesenchymal stem cells and in situ-forming gel: a multicentre, prospective, double-blind randomised controlled trial.
  Hideki Sudo; Takashi Miyakoshi; Yudai Watanabe; Yoichi M Ito; Kaoru Kahata; Khin Khin Tha; Nozomi Yokota; Hiroe Kato; Tomoko Terada; Norimasa Iwasaki; Teruyo Arato; Norihiro Sato; Toshiyuki Isoe
  BMJ open, 13, 2, e065476, 02 Feb. 2023, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, INTRODUCTION: In patients with combined lumbar spinal canal stenosis (LSCS), a herniated intervertebral disc (IVD) that compresses the dura mater and nerve roots is surgically treated with discectomy after laminoplasty. However, defects in the IVD after discectomy may lead to inadequate tissue healing and predispose patients to the development of IVD degeneration. Ultrapurified stem cells (rapidly expanding clones (RECs)), combined with an in situ-forming bioresorbable gel (dMD-001), have been developed to fill IVD defects and prevent IVD degeneration after discectomy. We aim to investigate the safety and efficacy of a new treatment method in which a combination of REC and dMD-001 is implanted into the IVD of patients with combined LSCS. METHODS AND ANALYSIS: This is a multicentre, prospective, double-blind randomised controlled trial. Forty-five participants aged 20-75 years diagnosed with combined LSCS will be assessed for eligibility. After performing laminoplasty and discectomy, participants will be randomised 1:1:1 into the combination of REC and dMD-001 (REC-dMD-001) group, the dMD-001 group or the laminoplasty and discectomy alone (control) group. The primary outcomes of the trial will be the safety and effectiveness of the procedure. The effectiveness will be assessed using visual analogue scale scores of back pain and leg pain as well as MRI-based estimations of morphological and compositional quality of the IVD tissue. Secondary outcomes will include self-assessed clinical scores and other MRI-based estimations of compositional quality of the IVD tissue. All evaluations will be performed at baseline and at 1, 4, 12, 24 and 48 weeks after surgery. ETHICS AND DISSEMINATION: This study was approved by the ethics committees of the institutions involved. We plan to conduct dissemination of the outcome data by presenting our data at national and international conferences, as well as through formal publication in a peer-reviewed journal. TRIAL REGISTRATION NUMBER: jRCT2013210076.
• Lumbar ossification of the ligamentum flavum reflects a strong ossification tendency of the entire spinal ligament.
  Kazuha Nakabachi; Tsutomu Endo; Masahiko Takahata; Ryo Fujita; Yoshinao Koike; Ryota Suzuki; Yuichi Hasegawa; Toshifumi Murakami; Katsuhisa Yamada; Hideki Sudo; Mohamad Alaa Terkawi; Ken Kadoya; Norimasa Iwasaki
  Scientific reports, 13, 1, 638, 638, 12 Jan. 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Patients with ossification of the ligamentum flavum (OLF) in the lumbar spine may be at high risk of developing concomitant ossification of the entire spinal ligament, but the etiology remains unclear. We investigated the propensity for spinal ligament ossification in asymptomatic subjects with lumbar OLF using the data of 595 Japanese individuals receiving medical check-ups, including computed tomography (CT) scanning. The severity of OLF (total number of intervertebral segments with OLF) of the entire spine on CT was quantified using an OLF index. Subjects with OLF were grouped according to this index: localized OLF (n = 138), intermediate OLF (n = 70), and extensive OLF (n = 31). The proportion of subjects with lumbar OLF increased with increasing OLF index (localized 13.7%, intermediate 41.4%, and extensive 70.9%). Multiple regression analysis found that lumbar OLF index was associated with thoracic OLF index, and co-existence of ossification of the posterior longitudinal ligament (OPLL) of the thoracic and lumbar spine. This study showed that subjects with more multilevel lumbar OLF were more likely to develop multilevel thoracic OLF and to have coexisting OPLL. Patients with lumbar OLF may be a distinctive subgroup with a strong tendency to ossification of the entire spinal ligament.
• Prediction of Cobb Angle Using Deep Learning Algorithm with Three-Dimensional Depth Sensor Considering the Influence of Garment in Idiopathic Scoliosis
  Yoko Ishikawa; Terufumi Kokabu; Katsuhisa Yamada; Yuichiro Abe; Hiroyuki Tachi; Hisataka Suzuki; Takashi Ohnishi; Tsutomu Endo; Daisuke Ukeba; Katsuro Ura; Masahiko Takahata; Norimasa Iwasaki; Hideki Sudo
  Journal of Clinical Medicine, 12, 2, 499, 499, MDPI AG, 07 Jan. 2023, [Peer-reviewed], [Last author, Corresponding author]
  Scientific journal, Adolescent idiopathic scoliosis (AIS) is the most common pediatric spinal deformity. Early detection of deformity and timely intervention, such as brace treatment, can help inhibit progressive changes. A three-dimensional (3D) depth-sensor imaging system with a convolutional neural network was previously developed to predict the Cobb angle. The purpose of the present study was to (1) evaluate the performance of the deep learning algorithm (DLA) in predicting the Cobb angle and (2) assess the predictive ability depending on the presence or absence of clothing in a prospective analysis. We included 100 subjects with suspected AIS. The correlation coefficient between the actual and predicted Cobb angles was 0.87, and the mean absolute error and root mean square error were 4.7° and 6.0°, respectively, for Adam’s forward bending without underwear. There were no significant differences in the correlation coefficients between the groups with and without underwear in the forward-bending posture. The performance of the DLA with a 3D depth sensor was validated using an independent external validation dataset. Because the psychological burden of children and adolescents on naked body imaging is an unignorable problem, scoliosis examination with underwear is a valuable alternative in clinics or schools.
• Identification of a Functional Susceptibility Variant for Adolescent Idiopathic Scoliosis that Upregulates Early Growth Response 1 (EGR1)-Mediated UNCX Expression.
  Yoshiro Yonezawa; Long Guo; Hisaya Kakinuma; Nao Otomo; Soichiro Yoshino; Kazuki Takeda; Masahiro Nakajima; Toshiyuki Shiraki; Yoji Ogura; Yohei Takahashi; Yoshinao Koike; Shohei Minami; Koki Uno; Noriaki Kawakami; Manabu Ito; Ikuho Yonezawa; Kei Watanabe; Takashi Kaito; Haruhisa Yanagida; Hiroshi Taneichi; Katsumi Harimaya; Yuki Taniguchi; Hideki Shigematsu; Takahiro Iida; Satoru Demura; Ryo Sugawara; Nobuyuki Fujita; Mitsuru Yagi; Eijiro Okada; Naobumi Hosogane; Katsuki Kono; Kazuhiro Chiba; Toshiaki Kotani; Tsuyoshi Sakuma; Tsutomu Akazawa; Teppei Suzuki; Kotaro Nishida; Kenichiro Kakutani; Taichi Tsuji; Hideki Sudo; Akira Iwata; Tatsuya Sato; Satoshi Inami; Masaya Nakamura; Morio Matsumoto; Chikashi Terao; Kota Watanabe; Hitoshi Okamoto; Shiro Ikegawa
  Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 38, 1, 144, 153, Jan. 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Adolescent idiopathic scoliosis (AIS) is a serious health problem affecting 3% of live births all over the world. Many loci associated with AIS have been identified by previous genome wide association studies, but their biological implication remains mostly unclear. In this study, we evaluated the AIS-associated variants in the 7p22.3 locus by combining in silico, in vitro, and in vivo analyses. rs78148157 was located in an enhancer of UNCX, a homeobox gene and its risk allele upregulated the UNCX expression. A transcription factor, early growth response 1 (EGR1), transactivated the rs78148157-located enhancer and showed a higher binding affinity for the risk allele of rs78148157. Furthermore, zebrafish larvae with UNCX messenger RNA (mRNA) injection developed body curvature and defective neurogenesis in a dose-dependent manner. rs78148157 confers the genetic susceptibility to AIS by enhancing the EGR1-regulated UNCX expression. © 2022 American Society for Bone and Mineral Research (ASBMR).
• Strong relationship between dyslipidemia and the ectopic ossification of the spinal ligaments.
  Tsutomu Endo; Masahiko Takahata; Ryo Fujita; Yoshinao Koike; Ryota Suzuki; Yuichi Hasegawa; Toshifumi Murakami; Misaki Ishii; Katsuhisa Yamada; Hideki Sudo; Norimasa Iwasaki
  Scientific reports, 12, 1, 22617, 22617, 30 Dec. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Obesity and metabolic disturbances are prevalent in ossification of the posterior longitudinal ligament (OPLL) and ossification of the ligamentum flavum (OLF); however, the involvement of dyslipidemia (DL) in OPLL/OLF remains uncertain. We investigated the association between dyslipidemia and OPLL/OLF using a dataset of 458 individuals receiving health screening tests, including computed tomography. Subjects were grouped according to the presence or location of OPLL/OLF: controls (no OPLL/OLF, n = 230), OLF (n = 167), cervical OPLL (n = 28), and thoracic OPLL (n = 33). They were also grouped according to the presence of dyslipidemia (DL[+], n = 215; DL[-], n = 243). The proportion of dyslipidemia in the OLF and OPLL groups was 1.6-2.2 times higher than that in the control group. The proportion of OLF and OPLL in the DL(+) group was significantly higher than that in the DL(-) group (OLF, 43% vs. 29%; cervical OPLL, 14.4% vs. 3.2%; thoracic OPLL, 11.1% vs. 3.7%). Multivariate logistic regression analysis showed an association between all ossification types and dyslipidemia. This study demonstrated an association of dyslipidemia with OPLL/OLF; further investigation on the causal relationship between dyslipidemia and ectopic spinal ligament ossification is warranted to develop a therapeutic intervention for OPLL/OLF.
• Effects of Posterior Spinal Correction and Fusion on Postural Stability in Patients with Adolescent Idiopathic Scoliosis.
  Satoshi Osuka; Hideki Sudo; Katsuhisa Yamada; Hiroyuki Tachi; Kentaro Watanabe; Fuma Sentoku; Takeshi Chiba; Norimasa Iwasaki; Masahiko Mukaino; Harukazu Tohyama
  Journal of clinical medicine, 12, 1, 29 Dec. 2022, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, The present study aimed to assess the effects of posterior spinal correction and fusion on postural stability in patients with adolescent idiopathic scoliosis (AIS). The study included 41 female patients with AIS at our institution. All patients performed three 10 s single-leg standing trials on a force plate. The center of pressure (COP) was measured preoperatively, and at 1 week and 6 months postoperatively. The postural stability parameters were absolute minimum time-to-boundary (TTB), mean of the minimum TTB, mean COP velocity, standard deviation, range, and 95% confidence ellipse area. One-way repeated analysis of variance or Friedman test was applied to the postural stability parameters. Multiple comparisons were performed using the Bonferroni correction. The absolute minimum TTB and the mean minimum TTB showed a significant increase 6 months post-operation as compared to preoperatively and 1 week postoperatively. The COP velocity significantly decreased at 6 months post-operation compared to preoperatively and 1 week postoperatively. These changes in postural stability indicate that spinal correction and fusion can be considered to improve postural stability during single-leg standing tests in the postoperative period.
• Posterolateral full-endoscopic debridement and irrigation is effective in treating thoraco-lumbar pyogenic spondylodiscitis, except in cases with large abscess cavities
  Katsuhisa Yamada; Masahiko Takahata; Ken Nagahama; Akira Iwata; Tsutomu Endo; Ryo Fujita; Hiroyuki Hasebe; Takashi Ohnishi; Hideki Sudo; Manabu Ito; Norimasa Iwasaki
  European Spine Journal, Springer Science and Business Media LLC, 24 Nov. 2022, [Peer-reviewed]
  Scientific journal
• Long-Term Clinical Course of Patients After Decompression and Posterior Instrumented Fusion Surgery for Thoracic Ossification of the Posterior Longitudinal Ligament: An Average Follow-Up of 18 years
  Masahiko Takahata; Tsutomu Endo; Yoshinao Koike; Kuniyoshi Abumi; Kota Suda; Ryo Fujita; Toshifumi Murakami; Hideki Sudo; Katsuhisa Yamada; Takashi Ohnishi; Katsuro Ura; Daisuke Ukeba; Norimasa Iwasaki
  Global Spine Journal, 219256822211355, 219256822211355, SAGE Publications, 15 Oct. 2022, [Peer-reviewed]
  Scientific journal, Study Design
  
  Retrospective observational study.
  
  Objectives
  
  To evaluate the long-term recurrence rates and functional status of patients with thoracic ossification of the posterior longitudinal ligament (OPLL) after decompression and posterior fusion surgery.
  
  Methods
  
  Thirty-seven consecutive patients who underwent posterior thoracic spine surgery at a single institution were retrospectively reviewed. The long-term neurological and functional outcomes of 25 patients who were followed up for ≥10 years after surgery were assessed. Factors associated with the recurrence of myelopathy were also analyzed.
  
  Results
  
  The mean preoperative Japanese Orthopaedic Association score was 3.7, which improved to 6.5 at postoperative year 2 and declined to 6.0 at a mean follow-up of 18 years. No patient experienced a relapse of myelopathy due to OPLL within the instrumented spinal segments. However, 15 (60%) patients experienced late neurological deterioration, 10 of whom had a relapse of myelopathy due to OPLL or ossification of the ligamentum flavum (OLF) in the region outside the primary operative lesion, while 4 developed myelopathy due to traumatic vertebral fracture of the ankylosed spine. Young age, a high body mass index, and lumbar OPLL are likely associated with late neurological deterioration.
  
  Conclusions
  
  Decompression and posterior instrumented fusion surgery is a reliable surgical procedure with stable long-term clinical outcomes for thoracic OPLL. However, as OPLL may progress through the spine, attention should be paid to the recurrence of paralysis due to OPLL or OLF in regions other than the primary operative lesion and vertebral fractures of the ankylosed spine after surgery for thoracic OPLL.
• Dual-Rod Instrumentation Via the Anterior Approach in Thoracolumbar and Lumbar Adolescent Idiopathic Scoliosis
  Hideki Sudo
  JBJS Essential Surgical Techniques, Oct. 2022, [Peer-reviewed], [Lead author, Last author, Corresponding author]
  Scientific journal
• Preliminary shape similarity analysis and standardization for pre-bent rod design for adult spinal deformity correction
  Soutome A; Kanai S; Date H; Kokabu T; Abe Y; Moridaira H; Taneichi H; Sudo H
  Comput Aided Des Appl, Aug. 2022, [Peer-reviewed], [Last author]
• Distinct progression pattern of ossification of the posterior longitudinal ligament of the thoracic spine versus the cervical spine: a longitudinal whole-spine CT study.
  Yuichiro Hisada; Tsutomu Endo; Yoshinao Koike; Masahiro Kanayama; Ryota Suzuki; Ryo Fujita; Katsuhisa Yamada; Akira Iwata; Hiroyuki Hasebe; Hideki Sudo; Norimasa Iwasaki; Masahiko Takahata
  Journal of neurosurgery. Spine, 1, 8, 04 Mar. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: Data regarding risk factors for the progression of ossification of the posterior longitudinal ligament (OPLL) in the thoracic spine are scarce. Therefore, in this study, the authors aimed to elucidate the difference in the radiographic progression pattern of OPLL and its risk factors between cervical and thoracic OPLL using longitudinally acquired whole-spine CT scans. METHODS: Overall, 123 patients with symptomatic OPLL who underwent repeated whole-spine CT examinations, with an average interval of 49 months (at least 3 years) between scans, were retrospectively reviewed. Progression of OPLL was assessed to compare the distribution of OPLL over the entire spine on the initial and final CT scans. Patients were divided into a cervical OPLL (C-OPLL) group and a thoracic OPLL (T-OPLL) group according to the location of the main lesion. The progression pattern of OPLL and its risk factors were compared between the two groups using the Student t-test or Mann-Whitney U-test. RESULTS: In the C-OPLL group, 15 (22.1%) of 68 patients had OPLL progression, of whom 12 patients (80.0%) had progression only in the cervical spine and 3 patients (20.0%) had progression in multiple regions (cervical and thoracic/lumbar). In the T-OPLL group, 16 (29.1%) of 55 patients had OPLL progression, of which 3 patients (18.8%) had progression only in the thoracic spine and 8 patients (50.0%) had progression in multiple regions. Young age was a common risk factor for OPLL progression regardless of the location of OPLL, and this trend was more pronounced in the T-OPLL group than in the C-OPLL group. High BMI, male sex, and multilevel, severe T-OPLL were identified as independent risk factors for progression of T-OPLL (OR 1.19, 95% CI 1.03-1.37; OR 10.5, 95% CI 1.39-81.94; and OR 1.24, 95% CI 1.16-1.45, respectively). CONCLUSIONS: Patients with T-OPLL are predisposed to diffuse progression of OPLL over the entire spine, whereas patients with C-OPLL are likely to have progression in only the cervical spine. Young age and high BMI are significant risk factors for OPLL progression, especially in patients with T-OPLL. Our study highlights the need for continued follow-up in patients with T-OPLL, especially in young patients and those with obesity, for early detection of spinal cord and cauda equina symptoms due to the progression of OPLL throughout the spine.
• Four-dimensional anatomical spinal reconstruction in thoracic adolescent idiopathic scoliosis
  Hideki Sudo
  JBJS Essential Surgical Techniques, Feb. 2022, [Peer-reviewed], [Lead author, Last author, Corresponding author]
  Scientific journal
• Causes of and Molecular Targets for the Treatment of Intervertebral Disc Degeneration: A Review.
  Takashi Ohnishi; Norimasa Iwasaki; Hideki Sudo
  Cells, 11, 3, 24 Jan. 2022, [International Magazine]
  English, Scientific journal, Intervertebral disc degeneration (IVDD) is a pathological condition that can lead to intractable back pain or secondary neurological deficits. There is no fundamental cure for this condition, and current treatments focus on alleviating symptoms indirectly. Numerous studies have been performed to date, and the major strategy for all treatments of IVDD is to prevent cell loss due to programmed or regulated cell death. Accumulating evidence suggests that several types of cell death other than apoptosis, including necroptosis, pyroptosis, and ferroptosis, are also involved in IVDD. In this study, we discuss the molecular pathway of each type of cell death and review the literature that has identified their role in IVDD. We also summarize the recent advances in targeted therapy at the RNA level, including RNA modulations through RNA interference and regulation of non-coding RNAs, for preventing cell death and subsequent IVDD. Therefore, we review the causes and possible therapeutic targets for RNA intervention and discuss the future direction of this research field.
• Combination of ultra-purified stem cells with an in situ-forming bioresorbable gel enhances intervertebral disc regeneration.
  Daisuke Ukeba; Katsuhisa Yamada; Takashi Suyama; Darren R Lebl; Takeru Tsujimoto; Takayuki Nonoyama; Hirokazu Sugino; Norimasa Iwasaki; Masatoki Watanabe; Yumi Matsuzaki; Hideki Sudo
  EBioMedicine, 76, 103845, 103845, 24 Jan. 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, BACKGROUND: Lumbar intervertebral disc (IVD) herniations are associated with significant disability. Discectomy is the conventional treatment option for IVD herniations but causes a defect in the IVD, which has low self-repair ability, thereby representing a risk of further IVD degeneration. An acellular, bioresorbable, and good manufacturing practice (GMP)-compliant in situ-forming gel, which corrects discectomy-associated IVD defects and prevents further IVD degeneration had been developed. However, this acellular matrix-based strategy has certain limitations, particularly in elderly patients, whose tissues have low self-repair ability. The aim of this study was to investigate the therapeutic efficacy of using a combination of newly-developed, ultra-purified, GMP-compliant, human bone marrow mesenchymal stem cells (rapidly expanding clones; RECs) and the gel for IVD regeneration after discectomy in a sheep model of severe IVD degeneration. METHODS: RECs and nucleus pulposus cells (NPCs) were co-cultured in the gel. In addition, RECs combined with the gel were implanted into IVDs following discectomy in sheep with degenerated IVDs. FINDINGS: Gene expression of NPC markers, growth factors, and extracellular matrix increased significantly in the co-culture compared to that in each mono-culture. The REC and gel combination enhanced IVD regeneration after discectomy (up to 24 weeks) in the severe IVD degeneration sheep model. INTERPRETATION: These findings demonstrate the translational potential of the combination of RECs with an in situ-forming gel for the treatment of herniations in degenerative human IVDs. FUNDING: Ministry of Education, Culture, Sports, Science, and Technology of Japan, Japan Agency for Medical Research and Development, and the Mochida Pharmaceutical Co., Ltd.
• Radical resection for solitary thoracic spinous-process metastasis: a case report and technical note.
  Chikako Ishii; Akira Iwata; Katsuhisa Yamada; Tsutomu Endo; Takeru Tsujimoto; Hideki Sudo; Ai Shimizu; Norimasa Iwasaki; Masahiko Takahata
  Spinal cord series and cases, 8, 1, 8, 8, 17 Jan. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal, INTRODUCTION: Bone metastases confined to the posterior elements of the spine are rarely treated, as there exist no established radical surgical treatment options for this area. Herein, we present a case report of and technical note on a patient who underwent radical resection for a metastatic tumor in the thoracic spinous process. CASE PRESENTATION: A 34-year-old male presented with a nasopharyngeal carcinoma with a solitary metastatic focus in the spinous process of the 10th thoracic vertebra. Imaging revealed that the tumor was confined to the spinous process and the surrounding soft tissues. No tumor was noted in the pedicles, vertebral body, and cortical bone on the ventral side of the lamina, as well as within the spinal canal. As treatment for this solitary metastatic lesion, we decided to perform radical resection with sufficient margins that would include the involved spinous process and all surrounding soft tissues exhibiting evidence of tumor infiltration. The posterior elements of the 9th-11th vertebrae, multifidus muscles, and skin were widely resected en bloc using a T-saw. The posterior elements of the spinal column were resected at the level of pedicles without full visualization of the involved dural sac. The tumor-infiltrated soft tissues surrounding the T10 vertebral spinous process were excised without full visualization of the tumor. Adjuvant therapy was not administered postoperatively. During the second year of follow-up, no signs of recurrence or metastasis were noted. DISCUSSION: Our proposed technique allows wide resection of a solitary focus of metastasis in the posterior elements of the spine.
• Association between obesity and ossification of spinal ligaments in 622 asymptomatic subjects: a cross-sectional study.
  Tsutomu Endo; Masahiko Takahata; Yoshinao Koike; Ryo Fujita; Ryota Suzuki; Yuichiro Hisada; Yuichi Hasegawa; Hisataka Suzuki; Katsuhisa Yamada; Akira Iwata; Hideki Sudo; Daisuke Yoneoka; Norimasa Iwasaki
  Journal of bone and mineral metabolism, 40, 2, 337, 347, 16 Jan. 2022, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, INTRODUCTION: Previous studies on patients with symptoms of spinal ligament ossification, including ossification of the posterior longitudinal ligament (OPLL) and ligamentum flavum (OLF), have not clarified whether obesity is a cause or consequence of these diseases and were limited by selection bias. Thus, we investigated the association between obesity and the prevalence of spinal ligament ossification in randomly selected asymptomatic subjects. MATERIALS AND METHODS: Between April 2020 and March 2021, 622 asymptomatic Japanese subjects who underwent computed tomography of neck to pelvis for medical check-up purposes were included. All subjects were divided into the following three groups: normal weight (body mass index [BMI] < 25 kg/m2), obese I (25 ≤ BMI < 30 kg/m2), and obese II (BMI ≥ 30 kg/m2). The relationship between factors affecting the presence of each spinal ligament ossification was evaluated using multivariate logistic regression analysis. RESULTS: The proportion of subjects with thoracic OPLL was significantly higher in the obese II group than in the other two groups (vs. normal weight, P < 0.001; vs. obese I, P < 0.001). BMI was associated with the prevalence of OLF, cervical OPLL, thoracic OPLL, and ossification of the anterior longitudinal ligament (OALL). BMI was most significantly associated with the prevalence of thoracic OPLL (β, 0.28; 95% confidence interval, 0.17-0.39). CONCLUSION: BMI was associated with the prevalence of OALL, cervical OPLL, thoracic OPLL, and OLF in asymptomatic subjects, suggesting that obesity is associated with the development of heterotopic ossification of the spinal ligaments.
• Biomaterials and Cell-Based Regenerative Therapies for Intervertebral Disc Degeneration with a Focus on Biological and Biomechanical Functional Repair: Targeting Treatments for Disc Herniation
  Yamada K; Iwasaki N; Sudo H
  Cells, 11, 4, Jan. 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Intervertebral disc (IVD) degeneration is a common cause of low back pain and most spinal disorders. As IVD degeneration is a major obstacle to the healthy life of so many individuals, it is a major issue that needs to be overcome. Currently, there is no clinical treatment for the regeneration of degenerated IVDs. However, recent advances in regenerative medicine and tissue engineering suggest the potential of cell-based and/or biomaterial-based IVD regeneration therapies. These treatments may be indicated for patients with IVDs in the intermediate degenerative stage, a point where the number of viable cells decreases, and the structural integrity of the disc begins to collapse. However, there are many biological, biomechanical, and clinical challenges that must be overcome before the clinical application of these IVD regeneration therapies can be realized. This review summarizes the basic research and clinical trials literature on cell-based and biomaterial-based IVD regenerative therapies and outlines the important role of these strategies in regenerative treatment for IVD degenerative diseases, especially disc herniation.
• Causes of and molecular targets for the treatment of intervertebral disc degeneration: a review
  Ohnishi T; Iwasaki N; Sudo H
  Cells, Jan. 2022, [Peer-reviewed], [Last author, Corresponding author]
  Scientific journal
• Surgical outcome prediction using a four-dimensional planning simulation system with finite element analysis incorporating pre-bent rods in adolescent idiopathic scoliosis: Simulation for spatiotemporal anatomical correction technique
  Tachi H; Kato K; Abe Y; Kokabu T; Yamada K; Iwasaki N; Sudo H
  Frontiers in Bioengineering and Biotechnology, 9, 746902, 746902, Oct. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, An optimal surgical strategy for adolescent idiopathic scoliosis (AIS) is to provide maximal deformity correction while preserving spinal mobile segments as much as possible and obtaining a balanced posture. From a spatiotemporal deformity correction standpoint, we recently showed that anatomical four-dimensional (4D) spinal correction could be accomplished by curving the rod. In the surgical procedure, two rods are bent identically to confirm spinal anatomical alignment without referring to the intraoperative alignment of the deformity. Therefore, anatomically designed rods have been developed as notch-free, pre-bent rods for easier anatomical reconstruction. In addition to providing the best spinal instrumentation configurations as pre-bent rods, prediction of surgical outcome along with its biomechanical impact can be obtained by simulation of the surgical procedures with computer modeling. However, an objective model that can simulate the surgical outcome in patients with AIS has not been completely elucidated. The present study aimed to compare simulated deformity corrections based on our newly developed spatiotemporal morphological 4D planning simulation system incorporating pre-bent rods and actual deformity corrections in patients with AIS. A consecutive series of 47 patients who underwent anatomical posterior correction for AIS curves were prospectively evaluated. After multilevel facetectomy, except for the lowest instrumented segment, 11 types of pre-bent rods were used. Patient demographic data, radiographic measurements, and sagittal rod angles were analyzed within 1 week of surgery. Our simulation system incorporating pre-bent rods showed a significant correlation with the actual postoperative spinal alignment. The present study demonstrated the feasibility of our simulation system and the ability to simulate the surgical procedure using the pre-bent rods. The simulation system can be used to minimize the differences between the optimal and possible outcomes related to the instrumentation levels and rod shapes. Preoperative assumption of rod shape and length can contribute to a reduction in operative time which decreases blood loss and risk of infection. The results of the finite element analysis in the simulation system measured for each individual patient would also provide a more realistic representation of the surgical procedures., 11449040
• Thrombocytopenia associated with unrecognized non-alcoholic fatty liver disease is an independent predictor of perioperative significant blood loss in cervical laminoplasty.
  Ryota Suzuki; Yoshinao Koike; Masahiro Ota; Tsutomu Endo; Yuichiro Hisada; Takeru Tsujimoto; Masahiro Kanayama; Yoichi M Ito; Hideki Sudo; Akira Iwata; Katsuhisa Yamada; Norimasa Iwasaki; Masahiko Takahata
  World neurosurgery, 155, e797-e804, 09 Sep. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: This study aimed to identify risk factors for significant blood loss (SBL) in cervical laminoplasty, especially regarding thrombocytopenia and coagulopathy resulting from non-alcoholic fatty liver disease (NAFLD). METHODS: We retrospectively investigated differences in patient background data, laboratory data at the time of admission, and surgery-related data of 317 patients who underwent cervical laminoplasty and were divided into SBL (estimated blood loss (EBL)+drainage(D) ≥ 500 g) and non-SBL (EBL+D < 500 g) groups. To evaluate liver status, we used the fibrous 4 (FIB-4) index and considered FIB-4 index ≥ 1.85 as a representative phenotype for NAFLD with liver fibrosis. In addition, the risk factor for perioperative SBL was investigated using multiple logistic regression analysis, and the cutoff value was calculated. RESULTS: Incidence of perioperative SBL in cervical laminoplasty was 7.3% (23/317). Compared to the non-SBL group, the SBL group demonstrated significantly lower platelet count (PLT), lower AST, longer operation time, and greater number of opened laminae. According to multivariate analysis, lower PLT and a greater number of opened laminae were identified as significant risk factors for perioperative SBL. The cutoff value of PLT for predicting SBL was determined to be 16.7 x 104 /μL using a receiver operating characteristic curve. The liver fibrosis group revealed significantly lower PLT and higher EBL+D than the non-liver fibrosis group, CONCLUSIONS: Thrombocytopenia is an independent predictor of perioperative SBL in cervical laminoplasty. Thus, patients with mild thrombocytopenia that may be associated with NAFLD must be carefully monitored to avoid perioperative SBL.
• Exploratory clinical trial on the safety and capability of dMD-001 in lumbar disc herniation: Study protocol for a first-in-human pilot study.
  Katsuhisa Yamada; Maeda Kenichiro; Yoichi M Ito; Fujio Inage; Toshiyuki Isoe; Nozomi Yokota; Osamu Sugita; Norihiro Sato; Khin Khin Tha; Norimasa Iwasaki; Teruyo Arato; Hideki Sudo
  Contemporary clinical trials communications, 23, 100805, 100805, Sep. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Herniated nucleus pulposus (NP), one of the most common diseases of the spine, is surgically treated by removing the sequestered NP. However, intervertebral disc (IVD) defects may remain after discectomy, leading to inadequate tissue healing and predisposing patients to IVD degeneration. An acellular, bioresorbable, ultra-purified alginate (UPAL) gel (dMD-001) implantation system can be used to fill any IVD defects in order to prevent IVD degeneration after discectomy. This first-in-human pilot study aims to determine the feasibility, safety, and perceived patient response to a combined treatment involving discectomy and UPAL gel implantation for herniated NP. We designed a one-arm, double-centre, open-label, pilot trial. The study started in November 2018 and will run until a sample of 40 suitable participants is established. Patients aged 20-49 years, diagnosed with isolated lumbar IVD herniation and scheduled for discectomy represent suitable candidates. All eligible participants who provide informed consent undergo standard discectomy followed by UPAL gel implantation. The primary outcomes of the trial will be the feasibility and safety of the procedure. Secondary outcomes will include self-assessed clinical scores and magnetic resonance imaging-based measures of morphological and compositional quality of the IVD tissue. Initial outcomes will be published at 24 weeks. Analysis of feasibility and safety will be performed using descriptive statistics. Both intention-to-treat and per-protocol analyses of treatment trends of effectiveness will be conducted., 12011290
• Polygenic Risk Score of Adolescent Idiopathic Scoliosis for Potential Clinical Use.
  Nao Otomo; Hsing-Fang Lu; Masaru Koido; Ikuyo Kou; Kazuki Takeda; Yukihide Momozawa; Michiaki Kubo; Yoichiro Kamatani; Yoji Ogura; Yohei Takahashi; Masahiro Nakajima; Shohei Minami; Koki Uno; Noriaki Kawakami; Manabu Ito; Tatsuya Sato; Kei Watanabe; Takashi Kaito; Haruhisa Yanagida; Hiroshi Taneichi; Katsumi Harimaya; Yuki Taniguchi; Hideki Shigematsu; Takahiro Iida; Satoru Demura; Ryo Sugawara; Nobuyuki Fujita; Mitsuru Yagi; Eijiro Okada; Naobumi Hosogane; Katsuki Kono; Masaya Nakamura; Kazuhiro Chiba; Toshiaki Kotani; Tsuyoshi Sakuma; Tsutomu Akazawa; Teppei Suzuki; Kotaro Nishida; Kenichiro Kakutani; Taichi Tsuji; Hideki Sudo; Akira Iwata; Kazuo Kaneko; Satoshi Inami; Yuta Kochi; Wei-Chiao Chang; Morio Matsumoto; Kota Watanabe; Shiro Ikegawa; Chikashi Terao
  Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 36, 8, 1481, 1491, Aug. 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Adolescent idiopathic scoliosis (AIS) is a common disease causing three-dimensional spinal deformity in as many as 3% of adolescents. Development of a method that can accurately predict the onset and progression of AIS is an immediate need for clinical practice. Because the heritability of AIS is estimated as high as 87.5% in twin studies, prediction of its onset and progression based on genetic data is a promising option. We show the usefulness of polygenic risk score (PRS) for the prediction of onset and progression of AIS. We used AIS genomewide association study (GWAS) data comprising 79,211 subjects in three cohorts and constructed a PRS based on association statistics in a discovery set including 31,999 female subjects. After calibration using a validation data set, we applied the PRS to a test data set. By integrating functional annotations showing heritability enrichment in the selection of variants, the PRS demonstrated an association with AIS susceptibility (p = 3.5 × 10-40 with area under the receiver-operating characteristic [AUROC] = 0.674, sensitivity = 0.644, and specificity = 0.622). The decile with the highest PRS showed an odds ratio of as high as 3.36 (p = 1.4 × 10-10 ) to develop AIS compared with the fifth in decile. The addition of a predictive model with only a single clinical parameter (body mass index) improved predictive ability for development of AIS (AUROC = 0.722, net reclassification improvement [NRI] 0.505 ± 0.054, p = 1.6 × 10-8 ), potentiating clinical use of the prediction model. Furthermore, we found the Cobb angle (CA), the severity measurement of AIS, to be a polygenic trait that showed a significant genetic correlation with AIS susceptibility (rg = 0.6, p = 3.0 × 10-4 ). The AIS PRS demonstrated a significant association with CA. These results indicate a shared polygenic architecture between onset and progression of AIS and the potential usefulness of PRS in clinical settings as a predictor to promote early intervention of AIS and avoid invasive surgery. © 2021 American Society for Bone and Mineral Research (ASBMR).
• In vivo deformation of anatomically pre-bent rods in thoracic adolescent idiopathic scoliosis.
  Hideki Sudo; Hiroyuki Tachi; Terufumi Kokabu; Katsuhisa Yamada; Akira Iwata; Tsutomu Endo; Masahiko Takahata; Yuichiro Abe; Norimasa Iwasaki
  Scientific reports, 11, 1, 12622, 12622, 16 Jun. 2021, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, Some surgical strategies can maintain or restore thoracic kyphosis (TK); however, next-generation surgical schemes for adolescent idiopathic scoliosis (AIS) should consider anatomical corrections. A four-dimensional correction could be actively achieved by curving the rod. Thus, anatomically designed rods have been developed as notch-free, pre-bent rods for easier anatomical reconstruction. This study aimed to compare the initial curve corrections obtained using notch-free rods and manually bent, notched rods for the anatomical reconstruction of thoracic AIS. Two consecutive series of 60 patients who underwent anatomical posterior correction for main thoracic AIS curves were prospectively followed up. After multilevel facetectomy, except for the lowest instrumented segment, either notch-free or notched rods were used. Patient demographic data, radiographic measurements, and sagittal rod angles were analyzed within 1 week after surgery. Patients with notch-free rods had significantly higher postoperative TK than patients with notched rods (P < .001), but both groups achieved three-dimensional spinal corrections and significantly increased postoperative rates of patients with T6-T8 TK apex (P = .006 for notch-free rods and P = .008 for notched rods). The rod deformation angle at the concave side was significantly lower in the notch-free rods than in the notched rods (P < .001). The notch-free, pre-bent rod can maintain its curvature, leading to better correction or maintenance of TK after anatomical spinal correction surgery than the conventional notched rod. These results suggest the potential benefits of anatomically designed notch-free, pre-bent rods over conventional, manually bent rods., 11449040
• Impact of multilevel facetectomy on segmental spinal flexibility in patients with thoracic adolescent idiopathic scoliosis.
  Terufumi Kokabu; Yuichiro Abe; Katsuhisa Yamada; Norimasa Iwasaki; Hideki Sudo
  Clinical biomechanics (Bristol, Avon), 83, 105296, 105296, Mar. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, BACKGROUND: The aim of this study was to intraoperatively assess the effects of multilevel facetectomy on segmental spinal flexibility in patients with thoracic adolescent idiopathic scoliosis. METHODS: Twenty patients who underwent posterior thoracic adolescent idiopathic scoliosis curve correction were evaluated. Compressive or distractive loaded force of 50N was applied on the handle of a compressor or distractor connected to the necks of pedicle screws inserted at T7 to T11. Segmental spinal flexibility rates were calculated based on the distance between screw heads under the loaded and unloaded conditions. In addition, the flexibility rates were obtained before and after multilevel facetectomy. FINDINGS: Absolute flexibility rates of all segments significantly increased after multilevel facetectomy under both compressive and distractive forces (P < 0.01). The absolute change in the flexibility rate was significantly higher at the concave side than at the convex side under both compressive (P < 0.01) and distractive loaded forces (P = 0.046). No significant correlation was found between change in the flexibility rates and preoperative Cobb angle or preoperative curve flexibility. INTERPRETATION: From a biomechanical point of view, multilevel facetectomy provides proper spinal flexibility to improve the correction rate of posterior adolescent idiopathic scoliosis surgery. The effects are higher at the concave side than at the convex side.
• An algorithm for using deep learning convolutional neural networks with three dimensional depth sensor imaging in scoliosis detection
  Terufumi Kokabu; Satoshi Kanai; Noriaki Kawakami; Koki Uno; Toshiaki Kotani; Teppei Suzuki; Hiroyuki Tachi; Yuichiro Abe; Norimasa Iwasaki; Hideki Sudo
  Spine J, Feb. 2021, [Peer-reviewed], [Last author, Corresponding author]
• Bone Marrow Aspirate Concentrate Combined with in Situ Forming Bioresorbable Gel Enhances Intervertebral Disc Regeneration in Rabbits.
  Daisuke Ukeba; Katsuhisa Yamada; Takeru Tsujimoto; Katsuro Ura; Takayuki Nonoyama; Norimasa Iwasaki; Hideki Sudo
  The Journal of bone and joint surgery. American volume, 103, 8, e31, 21 Jan. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, BACKGROUND: The current surgical procedure of choice for intervertebral disc (IVD) herniation is discectomy, which induces postoperative IVD degeneration. Thus, cell-based therapies, as a 1-step simple procedure, are desired because of the poor capacity of IVDs for self-repair. The aim of this study was to investigate the repair efficacy of ultra-purified alginate (UPAL) gels containing bone marrow aspirate concentrate (BMAC) for the treatment of discectomy-associated IVD degeneration in rabbits. METHODS: The mechanical properties of 3 types of gels-UPAL, UPAL containing bone marrow-derived mesenchymal stem cells (BMSCs), and UPAL containing BMAC-were evaluated. Forty rabbits were assigned to 5 groups: intact control, discectomy (to make the cavity), UPAL (implantation of the UPAL gel after discectomy), BMSCs-UPAL (implantation of a combination of autogenic BMSCs and UPAL gel after discectomy), and BMAC-UPAL (implantation of a combination of BMAC and UPAL gel after discectomy). The gels were implanted at 4 weeks after induction of IVD degeneration. At 4 and 12 weeks, magnetic resonance imaging (MRI) as well as histological and immunohistochemical analyses were performed to analyze IVD degeneration qualitatively and the viability of the implanted cells. RESULTS: There was no significant difference among the 3 types of gels in terms of the results of unconfined compression tests. The implanted cells survived for 12 weeks. The histological grades of the BMSCs-UPAL (mean and standard deviation, 2.50 ± 0.53; p < 0.001) and BMAC-UPAL (2.75 ± 0.64, p = 0.001) showed them to be more effective in preventing degeneration than UPAL gel alone (3.63 ± 0.52). The effectiveness of BMAC-UPAL was not significantly different from that of BMSCs-UPAL, except with respect to type-II collagen synthesis. CONCLUSIONS: BMAC-UPAL significantly enhanced the repair of IVD defects created by discectomy. This approach could be an effective therapeutic strategy owing to its simplicity and cost-effectiveness compared with cell therapy using culture-expanded BMSCs. CLINICAL RELEVANCE: Local administration of the BMAC combined with UPAL gel could be an effective therapeutic strategy to enhance IVD repair after discectomy.
• Ultra-purified alginate gel implantation decreases inflammatory cytokine levels, prevents intervertebral disc degeneration, and reduces acute pain after discectomy.
  Katsuro Ura; Katsuhisa Yamada; Takeru Tsujimoto; Daisuke Ukeba; Norimasa Iwasaki; Hideki Sudo
  Scientific reports, 11, 1, 638, 638, 12 Jan. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Lumbar intervertebral disc (IVD) herniation causes severe low back pain (LBP), which results in substantial financial and emotional strains. Despite the effectiveness of discectomy, there is no existing treatment for post-operative LBP induced by progressive IVD degeneration. Two key factors of LBP are intradiscal inflammation, indicated by tumour necrosis factor alpha (TNF-α) and interleukin-6 (IL-6), and sensory nerve ingrowth into the inner layer of the annulus fibrosus, triggered by nerve growth factor/high-affinity tyrosine kinase A (TrkA) signalling. In an animal models of discectomy, the bioresorbable ultra-purified alginate (UPAL) gel with an extremely low-toxicity has been effective in acellular tissue repair. We aimed to investigate whether UPAL gel can alleviate LBP using a rat nucleus pulposus (NP) punch model and a rabbit NP aspirate model. In both models, we assessed TNF-α and IL-6 production and TrkA expression within the IVD by immunohistochemistry. Further, histological analysis and behavioural nociception assay were conducted in the rat model. UPAL gel implantation suppressed TNF-α and IL-6 production, downregulated TrkA expression, inhibited IVD degeneration, and reduced nociceptive behaviour. Our results suggest the potential of UPAL gel implantation as an innovative treatment for IVD herniation by reducing LBP and preventing IVD degeneration after discectomy.
• Risk factors of multidrug-resistant pyogenic spondylitis in thoraco-lumbar spine: A retrospective study of 122 cases.
  Katsuhisa Yamada; Masahiko Takahata; Manabu Ito; Ken Nagahama; Akira Iwata; Tsutomu Endo; Hideki Sudo; Nobuhisa Ishiguro; Norimasa Iwasaki
  Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association, 27, 1, 95, 100, 06 Jan. 2021, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, BACKGROUND: The number of spinal infections has been increasing in developed countries due to the increase of aged or immunosuppressed patients. Spondylitis caused by multidrug-resistant (MDR) bacterial infection often become intractable and require long-term antibiotic therapy and multiple surgeries. Therefore, it is of great importance to understand risk factors for MDR spinal infections. The aim of this study was to elucidate the risk factors for MDR bacterial spondylitis. METHODS: A total of 122 patients (82 men, 40 women; average age: 63.8 y) with thoracic/lumbar spondylitis who underwent posterolateral full-endoscopic debridement and irrigation were included. The organisms detected by this endoscopic procedure were investigated, and the incidence and risk factors for MDR bacterial infection were retrospectively analyzed. RESULTS: Cultures of specimens obtained by endoscopic procedures were positive in 78 patients (63.9%). Among 68 isolated bacteria, MDR bacteria accounted for 47.1%. Multivariate analysis showed that significant risk factors for MDR bacterial infection included autoimmune connective tissue disease (P = 0.03) and central venous catheter (P = 0.02). The incidence of MDR bacteria in patients who were administered a broad-spectrum antibiotic for more than 1 month preoperatively was 64.0%, which was significantly higher than in patients who were administered a broad-spectrum antibiotic for less than 1 month and patients who were administered a narrow-spectrum antibiotic (P < 0.01, P < 0.01, respectively). CONCLUSIONS: The significant risk factors for MDR bacterial spondylitis included immunosuppressed conditions, such as autoimmune connective tissue disease, presence of central venous catheter, and longer administration periods of a broad-spectrum antibiotic. In patients with pyogenic spondylitis who could not be controlled with previous antibiotics and whose result of culture was negative, administration of anti-MRSA antibiotics would be considered when they have the risk factors identified in this study.
• Aggravation of ossified ligamentum flavum lesion is associated with the degree of obesity
  Endo T; Koike Y; Hisada Y; Fujita R; Suzuki R; Tanaka M; Tsujimoto T; Shimamura Y; Hasegawa Y; Kanayama M; Yamada K; Iwata A; Sudo H; Ishii M; Iwasaki N; Takahata M
  Global Spine Journal, 13, 5, 21925682211031514, 21925682211031514, 2021, [Peer-reviewed], [International Magazine]
  English, Scientific journal, STUDY DESIGN: Retrospective cross-sectional study. OBJECTIVES: There is insufficient data on the clinical features of ossification of the ligamentum flavum (OLF) of the thoracic spine and the risk of progression of ossified lesions. The link between obesity and ossification of the posterior longitudinal ligament (OPLL), which frequently coexists with OLF, has been demonstrated. However, the link between obesity and OLF has not been recognized. We aimed to determine the prevalence of obesity in thoracic OLF and whether the severity of OLF is associated with the degree of obesity. METHODS: A total of 204 symptomatic Japanese subjects with thoracic OLF and 136 subjects without spinal ligament ossification as controls were included. OLF subjects were divided into 3 groups: 1) localized OLF (OLF <2-intervertebral regions); 2) multilevel OLF (OLF ≥3-intervertebral regions); and 3) OLF + OPLL. The severity of OLF was quantified using the OLF index using computed tomography imaging of the entire spine. RESULTS: The proportion of severely obese subjects (BMI ≥ 30 kg/m2) was significantly higher both in the multilevel OLF group (25.5%) and the OLF + OPLL group (44.3%) than in the localized OLF group (3.6%) and the control group (1.4%) (P < 0.01). BMI, age, and coexistence of cervical OPLL and lumbar OLF were associated with thoracic OLF index in the multiple regression analysis. CONCLUSIONS: Our findings demonstrated that obesity is a distinct feature of multilevel OLF in the thoracic spine and that the severity of OLF is associated with the degree of obesity.
• Expert consensus on surgical treatment for adolescent idiopathic scoliosis in Japan.
  Hideyuki Arima; Tetsuro Ohba; Daisuke Kudo; Hideki Shigematsu; Takashi Kaito; Wataru Saito; Hideki Sudo; Shoji Seki; Mitsuru Yagi; Manabu Ito; Daisuke Sakai
  Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association, 26, 5, 765, 773, 18 Sep. 2020, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, BACKGROUND: Surgical treatment for adolescent idiopathic scoliosis (AIS) has changed significantly with the advent of new medical devices and surgical procedures. Today, pre- and postoperative management differs widely between institutions. The purpose of this study is to establish consensus regarding the surgical management of AIS in Japan through the use of a questionnaire survey of experienced spine deformity surgeons. METHODS: From February to March 2020, experienced spine deformity surgeons who perform more than 25 cases of AIS surgery annually were asked to respond to a questionnaire request regarding AIS surgical management formulated by the International Affairs Committee of the Japanese Scoliosis Society. For each of the questions, consensus was achieved upon a 70% agreement among respondents. RESULTS: Responses were received from 25 of the 32 (78%) experienced spine deformity surgeons. The average age of the responding surgeons was 52 years with an average practice experience of 28 year. Consensus was achieved on 74 (76%) of the 97 aspects of care presented in the questionnaire and is broken down as follows: 12 of 17 items for preoperative management, all 5 items for perioperative management, 11 of 14 items for surgical technique, 9 of 15 items for implant selection, 6 of 8 items for bone grafting, 7 of 10 items for blood conservation, 5 of 7 items for postoperative management, all 17 items for postoperative evaluation, and 2 of 4 items for aftercare. CONCLUSIONS: Expert consensus was achieved on 74 aspects of the surgical management of AIS in Japan. In implant selection and aftercare, consensus was obtained in less than 70% of the aspects, revealing differences in AIS management between institutions. These findings on AIS surgery in Japan, informed by expert opinion, will conceivably help spine deformity surgeons determine appropriate surgical management of AIS.
• Impact of lowest instrumented vertebra tilt and rotation on uninstrumented lumbar curve and L4 tilt in thoracic adolescent idiopathic scoliosis.
  Akira Iwata; Hideki Sudo; Kuniyoshi Abumi; Manabu Ito; Katsuhisa Yamada; Norimasa Iwasaki
  Journal of neurosurgery. Spine, 1, 9, 12 Jun. 2020, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, OBJECTIVE: Controversy exists regarding the effects of lowest instrumented vertebra (LIV) tilt and rotation on uninstrumented lumbar segments in adolescent idiopathic scoliosis (AIS) surgery. Because the intraoperative LIV tilt from the inferior endplate of the LIV to the superior sacral endplate is not stable after surgery, the authors measured the LIV angle of the instrumented thoracic spine as the LIV angle of the construct. This study aimed to evaluate the effects of the LIV angle of the construct and the effects of LIV rotation on the postoperative uninstrumented lumbar curve and L4 tilt in patients with thoracic AIS. METHODS: A retrospective correlation and multivariate analysis of a prospectively collected, consecutive, nonrandomized series of patients at a single institution was undertaken. Eighty consecutive patients with Lenke type 1 or type 2 AIS treated with posterior correction and fusion were included. Preoperative and 2-year postoperative radiographic measurements were the outcome measures for this study. Outcome variables were postoperative uninstrumented lumbar segments (LIV tilt, LIV translation, uninstrumented lumbar curve, thoracolumbar/lumbar [TL/L] apical vertebral translation [AVT], and L4 tilt). The LIV angle of the construct was measured from the orthogonal line drawn from the upper instrumented vertebra to the LIV. Multiple stepwise linear regression analysis was conducted between outcome variables and patient demographics/radiographic measurements. There were no study-specific biases related to conflicts of interest. RESULTS: Predictor variables for postoperative uninstrumented lumbar curve were the postoperative LIV angle of the construct, number of uninstrumented lumbar segments, and flexibility of TL/L curve. Specifically, a lower postoperative uninstrumented lumbar curve was predicted by a lower absolute value of the postoperative LIV angle of the construct (p < 0.0001). Predictor variables for postoperative L4 tilt were postoperative LIV rotation, preoperative L4 tilt, and preoperative uninstrumented lumbar curve. Specifically, a lower postoperative L4 tilt was predicted by a lower absolute value of postoperative LIV rotation (p < 0.0001). CONCLUSIONS: The LIV angle of the construct significantly affected the LIV tilt, uninstrumented lumbar curve, and TL/L AVT. LIV rotation significantly affected the LIV translation and L4 tilt.
• Mechanical Analysis of Notch-Free Pre-Bent Rods for Spinal Deformity Surgery.
  Katsuhisa Yamada; Hideki Sudo; Norimasa Iwasaki; Akihiko Chiba
  Spine, 45, 6, E312-E318, 15 Mar. 2020, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, STUDY DESIGN: Experimental study of spinal rod as per the American Society for Testing Materials (ASTM) F2193 methodology for static and dynamic four-point bending. OBJECTIVE: The hypotheses underlying this study were that the notch-free, curved rod would have a significantly higher ultimate load and fatigue strength compared with conventional notched curved rods. This study aimed to analyze the mechanical properties of notch-free curved rods compared with conventional notched rods. SUMMARY OF BACKGROUND DATA: The goal of instrumented spinal fusion in the management of spinal deformities is to realign the spine and maintain the correction and stability in order to obtain arthrodesis. Although rod curvature could play an important role, intraoperative contouring of the straight rod induces notches into the rod, leading to decreased fatigue strength. METHODS: Commercially produced titanium alloy (ϕ6.0 mm) and cobalt chromium alloy (ϕ5.5 mm) spinal rods were assessed by four-point bending tests in accordance with the ASTM F2193. RESULTS: Static four-point bending tests for the curved spinal rods showed that cobalt chromium alloy rods had significantly higher stiffness compared with titanium alloy rods. Notch-free cobalt chromium alloy rods had a significantly higher ultimate load than the conventional notched cobalt chromium alloy and titanium alloy rods. The dynamic four-point bending test showed that force/displacement at a minimum force at 2,500,000 cycles was larger in the notch-free cobalt chromium alloy rod than in the notched cobalt chromium alloy rod. CONCLUSION: The notch-free curved cobalt chromium alloy rod is likely to maintain its curvature after spinal deformity surgery with a decreased risk of breakage and could overcome the problems of the conventional notched rod such as breakage and spring-back. LEVEL OF EVIDENCE: N/A.
• Bone marrow mesenchymal stem cells combined with ultra-purified alginate gel as a regenerative therapeutic strategy after discectomy for degenerated intervertebral discs.
  Daisuke Ukeba; Hideki Sudo; Takeru Tsujimoto; Katsuro Ura; Katsuhisa Yamada; Norimasa Iwasaki
  EBioMedicine, 53, 102698, 102698, Mar. 2020, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, BACKGROUND: Because the regenerative ability of intervertebral discs (IVDs) is restricted, defects caused by discectomy may induce insufficient tissue repair leading to further IVD degeneration. An acellular bioresorbable biomaterial based on ultra-purified alginate (UPAL) gel was developed to fill the IVD cavity and prevent IVD degeneration. However, an acellular matrix-based strategy may have limitations, particularly in the elderly population, who exhibit low self-repair capability. Therefore, further translational studies involving product combinations, such as UPAL gel plus bone marrow-derived mesenchymal stem cells (BMSCs), are required to evaluate the regenerative effects of BMSCs embedded in UPAL gel on degenerated IVDs. METHODS: Rabbit BMSCs and nucleus pulposus cells (NPCs) were co-cultured in a three-dimensional (3D) system in UPAL gel. In addition, rabbit or human BMSCs combined with UPAL gel were implanted into IVDs following partial discectomy in rabbits with degenerated IVDs. FINDINGS: Gene expression of NPC markers, growth factors, and extracellular matrix was significantly increased in the NPC and BMSC 3D co-culture compared to that in each 3D mono-culture. In vivo, whereas UPAL gel alone suppressed IVD degeneration as compared to discectomy, the combination of BMSCs and UPAL gel exerted a more potent effect to induce IVD regeneration. Similar IVD regeneration was observed using human BMSCs. INTERPRETATION: These findings demonstrate the therapeutic potential of BMSCs combined with UPAL gel as a regenerative strategy following discectomy for degenerated IVDs. FUNDING: Ministry of Education, Culture, Sports, Science, and Technology of Japan, Japan Agency for Medical Research and Development, and the Mochida Pharmaceutical Co., Ltd.
• Long-term pulmonary function after posterior spinal fusion in main thoracic adolescent idiopathic scoliosis.
  Young Man Byun; Takahiro Iida; Katsuhisa Yamada; Kuniyoshi Abumi; Terufumi Kokabu; Akira Iwata; Norimasa Iwasaki; Hideki Sudo
  PloS one, 15, 6, e0235123, 2020, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, BACKGROUND: Adolescent idiopathic scoliosis (AIS) patients typically undergo surgical treatment as teenagers, follow-ups of >5 years are necessary to evaluate effects on peak pulmonary reserves. However, limited data is available regarding the long-term (>10 years) effects of surgical intervention on pulmonary function (PF) in patients with thoracic AIS. OBJECTIVE: To provide long-term (>10 years) information on the PF after posterior spinal fusion for treating main thoracic AIS. We especially investigated whether surgical correction for AIS led to impairment of the PF. METHODS: A total of 35 patients with main thoracic AIS treated with posterior spinal fusion were included. Radiographs and PF tests, which included measurements of absolute and percent-predicted values of forced vital capacity (FVC) and forced expiratory volume in 1 second (FEV1), were evaluated. RESULTS: Mean age at surgery was 14.9 years (12-19 years). Mean follow-up period was 15.1 years (10-24 years). Although the final postoperative FVC and FEV1 absolute values were higher than the preoperative values, the differences were not statistically significant (p = 0.22 and p = 0.08, respectively). Percent-predicted FVC and FEV1 values between preoperative and final postoperative measurements were not statistically different (p = 0.63 and p = 0.29, respectively). However, for the patients who presented with pulmonary impairment preoperatively, both the FVC and FEV1 significantly increased at the final follow-up (p = 0.01 and p = 0.01, respectively). CONCLUSIONS: Long-term results of AIS patients who underwent posterior spinal fusion in main thoracic curves demonstrated absolute and percent-predicted PF test values similar to preoperative measurements; thus, indicating that posterior spinal fusion did not decrease PF 15 years after the initial surgery. Instead, patients with severe preoperative pulmonary impairment might show some degree of improvement after surgery.
• Caspase-3 knockout inhibits intervertebral disc degeneration related to injury but accelerates degeneration related to aging.
  Takashi Ohnishi; Katsuhisa Yamada; Koji Iwasaki; Takeru Tsujimoto; Hideaki Higashi; Taichi Kimura; Norimasa Iwasaki; Hideki Sudo
  Scientific reports, 9, 1, 19324, 19324, 18 Dec. 2019, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Approximately 40% of people under 30 and over 90% of people 55 or older suffer from moderate-to-severe levels of degenerative intervertebral disc (IVD) disease in their lumbar spines. Surgical treatments are sometimes effective; however, the treatment of back pain related to IVD degeneration is still a challenge; therefore, new treatments are necessary. Apoptosis may be important in IVD degeneration because suppressing cell apoptosis inside the IVD inhibits degeneration. Caspase-3, the primary effector of apoptosis, may be a key treatment target. We analyzed caspase-3's role in two different types of IVD degeneration using caspase-3 knockout (Casp-3 KO) mice. Casp-3 KO delayed IVD degeneration in the injury-induced model but accelerated it in the age-induced model. Our results suggest that this is due to different pathological mechanisms of these two types of IVD degeneration. Apoptosis was suppressed in the IVD cells of Casp-3 KO mice, but cellular senescence was enhanced. This would explain why the Casp-3 KO was effective against injury-induced, but not age-related, IVD degeneration. Our results suggest that short-term caspase-3 inhibition could be used to treat injury-induced IVD degeneration.
• Bi-allelic loss of function variants of TBX6 causes a spectrum of malformation of spine and rib including congenital scoliosis and spondylocostal dysostosis.
  Nao Otomo; Kazuki Takeda; Shunsuke Kawai; Ikuyo Kou; Long Guo; Mitsujiro Osawa; Cantas Alev; Noriaki Kawakami; Noriko Miyake; Naomichi Matsumoto; Yukuto Yasuhiko; Toshiaki Kotani; Teppei Suzuki; Koki Uno; Hideki Sudo; Satoshi Inami; Hiroshi Taneichi; Hideki Shigematsu; Kei Watanabe; Ikuho Yonezawa; Ryo Sugawara; Yuki Taniguchi; Shohei Minami; Kazuo Kaneko; Masaya Nakamura; Morio Matsumoto; Junya Toguchida; Kota Watanabe; Shiro Ikegawa
  Journal of medical genetics, 56, 9, 622, 628, Sep. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: Congenital scoliosis (CS) is a common vertebral malformation. Spondylocostal dysostosis (SCD) is a rare skeletal dysplasia characterised by multiple vertebral malformations and rib anomalies. In a previous study, a compound heterozygosity for a null mutation and a risk haplotype composed by three single-nucleotide polymorphisms in TBX6 have been reported as a disease-causing model of CS. Another study identified bi-allelic missense variants in a SCD patient. The purpose of our study is to identify TBX6 variants in CS and SCD and examine their pathogenicity. METHODS: We recruited 200 patients with CS or SCD and investigated TBX6 variants. We evaluated the pathogenicity of the variants by in silico prediction and in vitro experiments. RESULTS: We identified five 16p11.2 deletions, one splice-site variant and five missense variants in 10 patients. In vitro functional assays for missense variants identified in the previous and present studies demonstrated that most of the variants caused abnormal localisation of TBX6 proteins. We confirmed mislocalisation of TBX6 proteins in presomitic mesoderm cells induced from SCD patient-derived iPS cells. In induced cells, we found decreased mRNA expressions of TBX6 and its downstream genes were involved in somite formation. All CS patients with missense variants had the risk haplotype in the opposite allele, while a SCD patient with bi-allelic missense variants did not have the haplotype. CONCLUSIONS: Our study suggests that bi-allelic loss of function variants of TBX6 cause a spectrum of phenotypes including CS and SCD, depending on the severity of the loss of TBX6 function.
• Effects of Intradiscal Injection of Local Anesthetics on Intervertebral Disc Degeneration in Rabbit Degenerated Intervertebral Disc.
  Ura K; Sudo H; Iwasaki K; Tsujimoto T; Ukeba D; Iwasaki N
  Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 37, 9, 1963, 1971, Sep. 2019, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Analgesic discoblock is widely used for the diagnosis or treatment of discogenic low back pain by injecting local anesthetics. The purpose of this study was to investigate the deleterious effects of local anesthetics on degenerated rabbit intervertebral disks (IVDs) using an organotypic culture model and in vivo long-term follow-up model. To induce IVD degeneration, a rabbit annular puncture model was used. For the organotypic culture model, degenerated IVDs were harvested 1 month after the initial annular puncture and cultured for 3 or 7 days after intradiscal injection of local anesthetics (1% lidocaine and 0.5% bupivacaine). To perform in vivo analysis, local anesthetics were injected into degenerated IVDs, and IVDs were prepared for histological analysis after 6 or 12 months. In the organotypic model, terminal deoxynucleotidyl transferase dUTP nick end labeling-positive nucleus pulposus (NP) cells were significantly increased in the bupivacaine group compared with the other groups. In the in vivo study, the number of NP cells was significantly decreased in the saline and local anesthetics groups compared with the untreated control and puncture-only groups. However, there was no significant difference among the saline, lidocaine, and bupivacaine groups. In addition, histological analysis showed no significant difference of IVD degeneration among the puncture-only, saline, lidocaine, and bupivacaine groups. Although bupivacaine induced apoptotic NP cell death in the organotypic culture model, in vivo observations did not show any definitive proof to suggest that local anesthetics were capable of promoting degeneration in the degenerated IVD, except for pressurized injection-induced damage. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:1963-1971, 2019.
• Genome-wide association study identifies 14 previously unreported susceptibility loci for adolescent idiopathic scoliosis in Japanese.
  Kou I; Otomo N; Takeda K; Momozawa Y; Lu HF; Kubo M; Kamatani Y; Ogura Y; Takahashi Y; Nakajima M; Minami S; Uno K; Kawakami N; Ito M; Yonezawa I; Watanabe K; Kaito T; Yanagida H; Taneichi H; Harimaya K; Taniguchi Y; Shigematsu H; Iida T; Demura S; Sugawara R; Fujita N; Yagi M; Okada E; Hosogane N; Kono K; Nakamura M; Chiba K; Kotani T; Sakuma T; Akazawa T; Suzuki T; Nishida K; Kakutani K; Tsuji T; Sudo H; Iwata A; Sato T; Inami S; Matsumoto M; Terao C; Watanabe K; Ikegawa S
  Nature communications, 10, 1, 3685, 3685, Aug. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Adolescent idiopathic scoliosis (AIS) is the most common pediatric spinal deformity. Several AIS susceptibility loci have been identified; however, they could explain only a small proportion of AIS heritability. To identify additional AIS susceptibility loci, we conduct a meta-analysis of the three genome-wide association studies consisting of 79,211 Japanese individuals. We identify 20 loci significantly associated with AIS, including 14 previously not reported loci. These loci explain 4.6% of the phenotypic variance of AIS. We find 21 cis-expression quantitative trait loci-associated genes in seven of the fourteen loci. By a female meta-analysis, we identify additional three significant loci. We also find significant genetic correlations of AIS with body mass index and uric acid. The cell-type specificity analyses show the significant heritability enrichment for AIS in multiple cell-type groups, suggesting the heterogeneity of etiology and pathogenesis of AIS. Our findings provide insights into etiology and pathogenesis of AIS.
• Three-dimensional depth sensor imaging to identify adolescent idiopathic scoliosis: a prospective multicenter cohort study.
  Terufumi Kokabu; Noriaki Kawakami; Koki Uno; Toshiaki Kotani; Teppei Suzuki; Yuichiro Abe; Kenichiro Maeda; Fujio Inage; Yoichi M Ito; Norimasa Iwasaki; Hideki Sudo
  Scientific reports, 9, 1, 9678, 9678, 04 Jul. 2019, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Adolescent idiopathic scoliosis is the most ordinary pediatric spinal disease that causes a three-dimensional deformity. Early detection of this potentially progressive deformity is considered crucial. The purpose of the present study was to report the potential for accurately diagnosis of adolescent idiopathic scoliosis using a newly developed, automated, noninvasive asymmetry-recognition system for the surface of the human back using a three-dimensional depth sensor. We included 170 subjects with suspected adolescent idiopathic scoliosis in this study. Outcomes measured included patient demographics, Cobbe angles from radiographic measurements, and asymmetry indexes. The coefficient of correlation between the asymmetry index and the Cobb angle was 0.85. For the prediction of scoliosis >10°, the area under the curve was 0.98, sensitivity was 0.97, specificity was 0.93, positive predictive value was 0.99, negative predictive value was 0.72, accuracy was 0.97, positive likelihood ratio was 13.55, and negative likelihood ratio was 0.04. The posterior test probability for the positive screen >10° was 98.9% if the asymmetry index was >1.268, three times in a row. This novel system automatically evaluated the back asymmetry. Therefore, this study demonstrates the outstanding discriminative ability of this newly developed system for deciding whether an examinee should undergo additional radiography to define scoliosis. This system can be used as an alternative to the forward bend test and scoliometer measurement in clinics. Future studies should seek to confirm these findings in a larger group and involve mass school scoliosis screening programs within the context of a multicenter trial.
• A multiethnic meta-analysis defined the association of rs12946942 with severe adolescent idiopathic scoliosis
  Kazuki Takeda; Ikuyo Kou; Nao Otomo; Anna Grauers; Yan-Hui Fan; Yoji Ogura; Yohei Takahashi; Yukihide Momozawa; Elisabet Einarsdottir; Juha Kere; Morio Matsumoto; Yong Qiu; You-Qiang Song; Paul Gerdhem; Kota Watanabe; Shiro Ikegawa; Noriaki Kawakami; Taichi Tsuji; Koki Uno; Teppei Suzuki; Manabu Ito; Shohei Minami; Toshiaki Kotani; Tsuyoshi Sakuma; Haruhisa Yanagida; Hiroshi Taneichi; Ikuho Yonezawa; Hideki Sudo; Kazuhiro Chiba; Naobumi Hosogane; Kotaro Nishida; Kenichiro Kakutani; Tsutomu Akazawa; Takashi Kaito; Kei Watanabe; Katsumi Harimaya; Yuki Taniguchi; Hideki Shigematsu; Satoru Demura; Takahiro Iida; Katsuki Kono; Eijiro Okada; Nobuyuki Fujita; Mitsuru Yagi
  JOURNAL OF HUMAN GENETICS, 64, 5, 493, 498, May 2019
  English, Scientific journal
• Simple method to construct process maps for additive manufacturing using a support vector machine
  Kenta Aoyagi; Hao Wang; Hideki Sudo; Akihiko Chiba
  Additive Manufacturing, 27, 353, 362, May 2019, [Peer-reviewed]
  Scientific journal
• Long-Term Changes in Vertebral Morphology After Cervical Spinal Fusion in Adolescent Pediatric Patients: Retrospective Case Series with up to a Minimum 12 Years of Follow-up.
  Yuichiro Abe; Katsuhisa Yamada; Kuniyoshi Abumi; Norimasa Iwasaki; Hideki Sudo
  World neurosurgery, 122, e765-e772, e772, Feb. 2019, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, OBJECTIVE: The mechanical alteration in the adolescent/pediatric cervical spine after spinal fusion remains unknown. The purpose of this study was to investigate morphologic changes in the cervical spine in adolescent/pediatric patients who underwent spinal fusion. METHODS: Ten adolescent/pediatric patients (9-18 years) who underwent cervical spinal fusion were included. The anteroposterior diameter (AP-D) of the vertebral body was evaluated using lateral radiographs. The AP-D ratio was defined as the ratio of the AP-D at final follow-up to the postoperative value. The kyphosis angles at the fused level and cervical spine (C2-C7) also were measured. RESULTS: The mean follow-up period was 20.0 years (range, 12-40 years). The AP-D was reduced in 4 patients and increased or remained unchanged in 6 patients. The AP-D reduction was usually seen at the middle of the fused levels and was remarkable in patients who underwent kyphosis correction using posterior instrumentation combined with anterior fusion. The AP-D ratio was significantly correlated to segments of anterior fusion (P = 0.029) and the kyphosis angle of the fused levels (P = 0.016). CONCLUSIONS: Cervical kyphosis correction using posterior instrumentation combined with endplate destruction by anterior bone grafting is a risk factor for atrophic morphologic changes in the vertebral body in adolescent/pediatric patients. Endplate destruction and instrumentation-induced stress shielding could alter bone remodeling.
• An international meta-analysis confirms the association of BNC2 with adolescent idiopathic scoliosis
  Yoji Ogura; Kazuki Takeda; Ikuyo Kou; Anas Khanshour; Anna Grauers; Hang Zhou; Gang Liu; Yan-Hui Fan; Taifeng Zhou; Zhihong Wu; Yohei Takahashi; Morio Matsumoto; Noriaki Kawakami; Taichi Tsuji; Koki Uno; Teppei Suzuki; Manabu Ito; Shohei Minami; Toshiaki Kotani; Tsuyoshi Sakuma; Haruhisa Yanagida; Hiroshi Taneichi; Ikuho Yonezawa; Hideki Sudo; Kazuhiro Chiba; Naobumi Hosogane; Kotaro Nishida; Kenichiro Kakutani; Tsutomu Akazawa; Takashi Kaito; Kei Watanabe; Katsumi Harimaya; Yuki Taniguchi; Hideki Shigematsu; Satoru Demura; Takahiro Iida; Katsuki Kono; Eijiro Okada; Nobuyuki Fujita; Mitsuru Yagi; Masaya Nakamura; Lori A. Karol; Karl E. Rathjen; Daniel J. Sucato; John G. Birch; Charles E. Johnston; Benjamin S. Richards; Brandon Ramo; Amy L. McIntosh; John A. Herring; Todd A. Milbrandt; Vishwas R. Talwakar; Henry J. Iwinski; Ryan D. Muchow; J. Channing Tassone; X. C. Liu; Richard Shindell; William Schrader; Craig Eberson; Anthony Lapinsky; Randall Loder; Joseph Davey; Elisabet Einarsdottir; Juha Kere; Dongsheng Huang; Guixing Qiu; Leilei Xu; Yong Qiu; Carol A. Wise; You-Qiang Song; Nan Wu; Peiqiang Su; Paul Gerdhem; Kota Watanabe; Shiro Ikegawa
  Scientific Reports, 8, 1, Nature Publishing Group, 01 Dec. 2018, [Peer-reviewed]
  English, Scientific journal
• Automated noninvasive detection of idiopathic scoliosis in children and adolescents: A principle validation study.
  Sudo H; Kokabu T; Abe Y; Iwata A; Yamada K; Ito YM; Iwasaki N; Kanai S
  Scientific reports, 8, 1, 17714, 17714, Dec. 2018, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, Idiopathic scoliosis is the most common pediatric musculoskeletal disorder that causes a three-dimensional deformity of the spine. Early detection of this progressive aliment is essential. The aim of this study is to determine outcomes using a newly developed automated asymmetry-evaluation system for the surface of the human back using a three-dimensional depth sensor. Seventy-six human subjects suspected to have idiopathic scoliosis were included in this study. Outcome measures include patient demographics, radiographic measurements, and asymmetry indexes defined in the automated asymmetry-recognition system. The mean time from scanning to analysis was 1.5 seconds. For predicting idiopathic scoliosis of greater than 25°, the area under the curve was 0.96, sensitivity was 0.97, and specificity was 0.88. The coefficient of variation for repeatability analyses using phantom models was 1-4%. The intraclass correlation coefficient obtained for intra-observer repeatability for human subjects was 0.995. The system three-dimensionally scans multiple points on the back, enabling an automated evaluation of the back's asymmetry in a few seconds. This study demonstrated discriminative ability in determining whether an examinee requires an additional x-ray to confirm diagnosis.
• Postoperative translation of the upper instrumented vertebra in thoracic adolescent idiopathic scoliosis.
  Katsuhisa Yamada; Hideki Sudo; Kiyoshi Kaneda; Yasuhiro Shono; Yuichiro Abe; Norimasa Iwasaki
  Journal of neurosurgery. Pediatrics, 22, 6, 694, 700, 01 Dec. 2018, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, OBJECTIVEThe aim of this retrospective study was to analyze the influence of upper instrumented vertebra (UIV) translation from the C7 plumb line (C7PL) on the long-term postoperative results of patients with main thoracic (MT) adolescent idiopathic scoliosis (AIS).METHODSTwenty-five patients had been treated surgically for AIS with a Lenke type 1 curve and had been followed up for a mean period of 18.2 years. Radiographic parameters, pulmonary function measurements, and clinical outcomes were compared between the patients (n = 15) with UIV translation < 20 mm and those (n = 10) with UIV translation ≥ 20 mm at the final follow-up. Correlations between UIV translation and radiographic or pulmonary function parameters were analyzed.RESULTSPatients with ≥ 20 mm UIV translation at the final follow-up had a significantly larger preoperative UIV translation than that in the patients with < 20 mm UIV translation at follow-up. The former group also had a significantly lower correction rate of the MT curve, higher chest cage ratio, and lower radiographic shoulder height (p = 0.01, 0.005, and 0.025, respectively) at the final follow-up. The Scoliosis Research Society (SRS)-30 Questionnaire scores were equivalent between the two groups. Correlation analysis showed that the following parameters were significantly associated with UIV translation: MT curve correction rate (r = -0.481, p = 0.015), chest cage ratio (r = 0.673, p < 0.001), and percent-predicted forced expiratory volume in 1 second (r = -0.455, p = 0.033).CONCLUSIONSThe UIV translation should be considered an important factor that influences postoperative results. In MT AIS patients whose preoperative upper end vertebra (UEV) is distant from the C7PL, the UIV should be selected above the UEV to prevent large UIV translation at the postoperative follow-up.
• 3D Asymmetry Analysis of Human’s Back Surface for Early Screening of Idiopathic Scoliosis
  Satoshi Kanai; Hideki Sudo; Terufumi Kokabu; Hiroshi Nagaeda; Takayuki Hayashi; Hajime Ohta
  International Conference on Precision Engineering (ICPE2018), D-5-2, Nov. 2018, [Peer-reviewed]
  English, International conference proceedings
• A novel technique of cervical pedicle screw placement with a pilot screw under the guidance of intraoperative 3D imaging from C-arm cone-beam CT without navigation for safe and accurate insertion.
  Masahiko Takahata; Katsuhisa Yamada; Iwata Akira; Tsutomu Endo; Hideki Sudo; Hidetoki Yokoyama; Norimasa Iwasaki
  European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, 27, 11, 2754, 2762, Nov. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, PURPOSE: The cervical pedicle screw (CPS) requires careful and accurate placement because of the critical risk for neurovascular injury. This study aimed to introduce and evaluate the safety and efficacy of a new CPS placement technique using intraoperative C-arm cone-beam CT (CBCT) and a pilot screw without navigation system. METHODS: This was a case-control study to compare the accuracy and safety of intraoperative C-arm CBCT-guided CPS placement with freehand CPS placement under lateral fluoroscopy using control data from a previous multicenter study. A total of 166 CPSs were inserted under intraoperative C-arm CBCT guidance in 48 consecutive patients (20 rheumatoid arthritis, 16 degenerative spinal disorders, 6 spinal tumor, 2 congenital deformity, 2 ossification of posterior longitudinal ligament, and 2 fracture dislocation). Accuracy and safety of CPS placement were assessed. RESULTS: The overall malposition rate was 2.4% (4 screws in grade 1: malposition by less than half-screw diameter, 0 in grade 2: malposition by more than half-screw diameter), which was significantly lower than the reported malposition rate of 14.8% in lateral fluoroscopy-guided freehand placement. There were no complications directly related to CPS insertion. The average estimated effective radiation dose per surgery was 14.7 mSv. CONCLUSIONS: The novel technique enables intraoperative adjustment of the trajectory of the CPS as well as confirmation of the CPS path before penetrating the isthmus of the pedicle, resulting in accurate and safe CPS placement, which outweighs the demerits of radiation exposure. These slides can be retrieved under Electronic Supplementary Material.
• An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair: A preclinical proof-of-concept study.
  Takeru Tsujimoto; Hideki Sudo; Masahiro Todoh; Katsuhisa Yamada; Koji Iwasaki; Takashi Ohnishi; Naoki Hirohama; Takayuki Nonoyama; Daisuke Ukeba; Katsuro Ura; Yoichi M Ito; Norimasa Iwasaki
  EBioMedicine, 37, 521, 534, Nov. 2018, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, BACKGROUND: The current surgical procedure of choice for lumbar intervertebral disc (IVD) herniation is discectomy. However, defects within IVD produced upon discectomy may impair tissue healing and predispose patients to subsequent IVD degeneration. This study aimed to investigate whether the use of an acellular bioresorbable ultra-purified alginate (UPAL) gel implantation system is safe and effective as a reparative therapeutic strategy after lumbar discectomy. METHODS: Human IVD cells were cultured in a three-dimensional system in UPAL gel. In addition, lumbar spines of sheep were used for mechanical analysis. Finally, the gel was implanted into IVD after discectomy in rabbits and sheep in vivo. FINDINGS: The UPAL gel was biocompatible with human IVD cells and promoted extracellular matrix production after discectomy, demonstrating sufficient biomechanical characteristics without material protrusion. INTERPRETATION: The present results indicate the safety and efficacy of UPAL gels in a large animal model and suggest that these gels represent a novel therapeutic strategy after discectomy in cases of lumbar IVD herniation. FUND: Grant-in-Aid for the Ministry of Education, Culture, Sports, Science, and Technology of Japan, Japan Agency for Medical Research and Development, and the Mochida Pharmaceutical Co., Ltd.
• Late Subaxial Lesion after Overcorrected Occipitocervical Reconstruction in Patients with Rheumatoid Arthritis.
  Iwata A; Abumi K; Takahata M; Sudo H; Yamada K; Endo T; Iwasaki N
  Asian spine journal, 13, 2, 181, 188, Nov. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, STUDY DESIGN: Retrospective case-control study, level 4. PURPOSE: To clarify the risk factors for late subaxial lesion after occipitocervical (O-C) reconstruction. We examined cases requiring fusion-segment-extended (FE) reconstruction in addition to/after O-C reconstruction. OVERVIEW OF LITERATURE: Patients with rheumatoid arthritis (RA) frequently require O-C reconstruction surgery for cranio-cervical lesions. Acceptable outcomes are achieved via indirect decompression using cervical pedicle screws and occipital plate-rod systems. However, late subaxial lesions may develop occasionally following O-C reconstruction. METHODS: O-C reconstruction using cervical pedicle screws and occipital plate-rod systems was performed between 1994 and 2007 in 113 patients with RA. Occipito-atlanto-axial (O-C2) reconstruction was performed for 89 patients, and occipito-subaxial cervical (O-under C2) reconstruction was performed for 24 patients. We reviewed the cases of patients requiring FE reconstruction (fusion extended group, FEG) and 26 consecutive patients who did not require FE reconstruction after a follow-up of >5 years (non-fusion extended group, NEG) as controls. RESULTS: FE reconstructions were performed for nine patients at an average of 45 months (range, 24-180 months) after O-C reconstruction. Of the 89 patients, three (3%) underwent FE reconstruction in cases of O-C2 reconstruction. Of the 24 patients, five (21%) underwent FE reconstruction in cases of O-under C2 reconstruction (p=0.003, Fisher exact test). Age, sex, RA type, and neurological impairment stage were not significantly different between FEG and NEG. O-under C2 reconstruction, larger correction angle (4° per number of unfixed segment), and O-C7 angle change after O-C reconstruction were the risk factors for late subaxial lesions on radiographic assessment. CONCLUSIONS: Overcorrection of angle at fusion segments requiring O-C7 angle change was a risk factor for late subaxial lesion in patients with RA with fragile bones and joints. Correction should be limited, considering the residual mobility of the cervical unfixed segments.
• Screening of known disease genes in congenital scoliosis.
  Takeda K; Kou I; Mizumoto S; Yamada S; Kawakami N; Nakajima M; Otomo N; Ogura Y; Miyake N; Matsumoto N; Kotani T; Sudo H; Yonezawa I; Uno K; Taneichi H; Watanabe K; Shigematsu H; Sugawara R; Taniguchi Y; Minami S; Nakamura M; Matsumoto M; Japan Early Onset; Scoliosis Research Group; Watanabe K; Ikegawa S
  Molecular genetics & genomic medicine, 6, 6, 966, 974, Sep. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: Congenital scoliosis (CS) is defined as a lateral curvature of the spine due to the vertebral malformations and has an incidence of 0.5-1/1,000 births. We previously examined TBX6 in Japanese CS patients and revealed that approximately 10% of CS was caused by TBX6 mutations. However, the genetic cause of remaining CS is unknown. METHODS: We recruited 78 CS patients without TBX6 mutations and major comorbidities, and investigated the genes previously reported to be associated with CS and congenital vertebral malformations by whole-exome sequencing. RESULTS: We identified the compound heterozygous missense variants in LFNG in one patient. No likely disease-causing variants were identified in other patients, however. LFNG encodes a GlcNAc-transferase. The LFNG variants showed loss of their enzyme function. CONCLUSIONS: A LFNG mutation is reported in a case of spondylocostal dysostosis (SCD), a skeletal dysplasia with severe malformations of vertebra and rib. The CS patient with LFNG mutations had multiple vertebral malformations including hemivertebrae, butterfly vertebrae, and block vertebrae, and rib malformations. LFNG mutations may cause a spectrum of phenotypes including CS and SCD. The current list of known disease genes could explain only a small fraction of genetic cause of CS.
• Identification of optimized rod shapes to guide anatomical spinal reconstruction for adolescent thoracic idiopathic scoliosis.
  Kokabu T; Kanai S; Abe Y; Iwasaki N; Sudo H
  Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 36, 12, 3219, 3224, Jul. 2018, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
  English, Scientific journal, Adolescent idiopathic scoliosis (AIS), the most common pediatric musculoskeletal disorder, causes a three-dimensional deformity of the spine. Although rod curvature could play an important role in anatomical spinal reconstruction in patients with thoracic AIS, intraoperative contouring of the straight rod induces notches into the rod, leading to decreased fatigue strength. Here, we analyzed pre-bent rod geometries from 46 intraoperative tracings of the rod geometry, which can provide anatomical spinal reconstruction in patients with thoracic AIS. The center point clouds of the rod shapes were extracted and approximated as arcs and straight lines. The difference between the center point clouds were evaluated using the iterative closest point methods. When the rod shapes were divided into six groups based on length followed by hierarchical cluster analysis, 10 representative rod shapes were obtained with a difference value of 5 mm. Thus, we identified optimized rod shapes to guide anatomical spinal reconstruction for thoracic AIS, which will reduce not only the risk of rod breakage but also operation time, leading to decreased patient burden. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:3219-3224, 2018.
• A Replication Study for the Association of rs11190870 With Curve Severity in Adolescent Idiopathic Scoliosis in Japanese.
  Yohei Takahashi; Ikuyo Kou; Yoji Ogura; Atsushi Miyake; Kazuki Takeda; Masahiro Nakajima; Shohei Minami; Noriaki Kawakami; Koki Uno; Manabu Ito; Ikuho Yonezawa; Takashi Kaito; Haruhisa Yanagida; Kei Watanabe; Hiroshi Taneichi; Katsumi Harimaya; Yuki Taniguchi; Toshiaki Kotani; Taichi Tsuji; Teppei Suzuki; Hideki Sudo; Nobuyuki Fujita; Mitsuru Yagi; Kazuhiro Chiba; Katsuki Kono; Tsuyoshi Sakuma; Tsutomu Akazawa; Kotaro Nishida; Kenichiro Kakutani; Hideki Shigematsu; Takahiro Iida; Satoru Demura; Naobumi Hosogane; Eijiro Okada; Masaya Nakamura; Morio Matsumoto; Kota Watanabe; Shiro Ikegawa
  Spine, 43, 10, 688, 692, 15 May 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, STUDY DESIGN: Case-only study. OBJECTIVE: The aim of this study was to confirm the association of rs11190870 with adolescent idiopathic scoliosis (AIS) severity in Japanese patients with AIS. SUMMARY OF BACKGROUND DATA: Although the association of rs11190870 with AIS susceptibility is replicated in multiple ethnics, the association of rs11190870 with curve severity is controversial. Since the previous studies are of small, we performed a replication study using far larger number of patients than previous studies. METHODS: A total of 1860 Japanese patients with AIS who had reached skeletal maturity or undergone surgical fusion were included in the study. We evaluated the association between rs11190870 and AIS progression for the entire group, and then for patients grouped according to a severe curve (a Cobb angle of ≥40°) or mild curve (a Cobb angle <30°). Because braces could affect the results of the present study, patients in the mild-curve group were divided according to whether or not they had worn a brace. We then evaluated associations between rs11190870 genotype and curve severity in these groups. RESULTS: The mean Cobb angles were 54.8° ± 12.1° in the severe-curve group and 24.4° ± 4.0° in the mild-curve group. The difference in rs11190870 risk-allele frequency between the severe- and mild-curve groups was evaluated. No significant differences were observed. We then examined the association of rs11190870 risk-allele frequency between patients in the mild- and severe-curve groups using the χ test for three models, and found a marginal association between rs11190870 and curve severity in the dominant model (P = 0.035, odds ratio = 1.51). CONCLUSION: We found no association between rs11190870 and curve severity using the criteria of previous study. However, we found a marginal association between rs11190870 and curve severity. Large-scale replication studies that consider skeletal maturity and brace history, including replication studies in other ethnic groups, would be helpful for clarifying the association. LEVEL OF EVIDENCE: 4.
• Impact of Multilevel Facetectomy and Rod Curvature on Anatomical Spinal Reconstruction in Thoracic Adolescent Idiopathic Scoliosis.
  Sudo H; Abe Y; Kokabu T; Kuroki K; Iwata A; Iwasaki N
  Spine, 43, 19, E1135, E1142, Mar. 2018, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, STUDY DESIGN: A prospective, nonrandomized study. OBJECTIVE: The aim of this study was to assess surgical outcomes of multilevel facetectomy and rod curvature with simultaneous double-rod rotation technique for anatomical spinal reconstruction in thoracic adolescent idiopathic scoliosis (AIS). SUMMARY OF BACKGROUND DATA: Although some surgical techniques maintain or restore thoracic kyphosis (TK), next-generation strategies for thoracic AIS should include corrections in three anatomical planes. METHODS: The study included 39 consecutive patients with Lenke 1 or Lenke 2 thoracic AIS treated at our institution. After all-level facetectomy at instrumentation level, except for the lowest intervertebral segment, two rods were identically bent to guide postoperative anatomical TK without reference to the intraoperative coronal alignment of the AIS deformity. Outcome measures included patient demographics, radiographic measurements, and Scoliosis Research Society (SRS) questionnaire scores. RESULTS: After 2 years of follow-up, the average main thoracic Cobb angle correction rate was 83.5%, and the final correction loss was 2.2°. The average preoperative TK (T5-T12) significantly increased from 13.2° to 24.6° (P < 0.001) at final follow-up. The percentage of patients with a T6-T8 location of the TK apex significantly increased from 51.3% preoperatively to 87.2% at final follow-up. The average preoperative vertebral rotation angle significantly decreased from 18.7° to 12.8° postoperatively (P < 0.001). The average preoperative total SRS questionnaire score significantly increased from 3.5 to 4.5 (P < 0.001) at final follow-up. There was no implant breakage and vascular and neurologic complications, with all patients demonstrating solid fusion at final follow-up. CONCLUSION: Multilevel facetectomy and rod curvature play an important role in anatomical spinal reconstruction in patients with thoracic AIS. From the spatiotemporal point of view, four-dimensional correction could be actively performed by rod curvature under multilevel facetectomy and is expected to obtain an anatomical thoracic spine postoperatively, indicating that an anatomically designed rod could be supplied as a pre-bent rod. LEVEL OF EVIDENCE: 3.
• Age-related spontaneous lumbar intervertebral disc degeneration in a mouse model
  Takashi Ohnishi; Hideki Sudo; Takeru Tsujimoto; Norimasa Iwasaki
  Journal of Orthopaedic Research, 36, 1, 224, 232, John Wiley and Sons Inc., 01 Jan. 2018, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• 18F-FDG-PET/CT localizes intervertebral disc space infection following posterior lumbar interbody fusion surgery leading to successful retention of percutaneously inserted pedicle screws: a case report.
  Haraya K; Yamada K; Kokabu T; Iwata A; Endo T; Sudo H; Iwasaki N; Takahata M
  Spinal cord series and cases, 4, 81, 81, 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Introduction: Postoperative infection is a potentially devastating complication of spine surgery and an appropriate strategy and timely decision-making are essential for successful treatment of deep surgical site infection (SSI) after spinal instrumentation surgeries. However, there is a lack of consensus on implant removal or retention. We report on a case of deep SSI after posterior lumbar interbody fusion (PLIF) surgery in which we achieved clinical cure by debridement and removal of the interbody fusion cage without removing the percutaneously inserted pedicle screws (PPS). Case presentation: A case was a 53-year-old woman with deep SSI after PLIF surgery using the PPS system at the L4-5 level. Computed tomography (CT) showed no clear radiolucent line around the screws and 18F-fluorodeoxyglucose positron emission tomography (FDG-PET)/CT demonstrated abnormal FDG uptake around the cages and no uptake around the pedicle screws. Intervertebral cages were removed and iliac bone grafts were inserted between the vertebral bodies, without removing the pedicle screws. The infection was cleared and bone fusion was achieved after the revision surgery. Discussion: Targeting active infection using FDG-PET/CT is considered useful in narrowing the surgical margins and determining whether to preserve instrumentation in revision surgery after SSI. PLIF using the PPS system could be useful in preventing the easy spread of infection from the intervertebral space to the insertion point of PPS through the interstitial space.
• A functional variant in MIR4300HG, the host gene of microRNA MIR4300 is associated with progression of adolescent idiopathic scoliosis
  Yoji Ogura; Ikuyo Kou; Yohei Takahashi; Kazuki Takeda; Shohei Minami; Noriaki Kawakami; Koki Uno; Manabu Ito; Ikuho Yonezawa; Takashi Kaito; Haruhisa Yanagida; Kei Watanabe; Hiroshi Taneichi; Katsumi Harimaya; Yuki Taniguchi; Toshiaki Kotani; Taichi Tsuji; Teppei Suzuki; Hideki Sudo; Nobuyuki Fujita; Mitsuru Yagi; Kazuhiro Chiba; Michiaki Kubo; Yoichiro Kamatani; Masaya Nakamura; Morio Matsumoto; Kota Watanabe; Shiro Ikegawa
  HUMAN MOLECULAR GENETICS, 26, 20, 4086, 4092, Oct. 2017, [Peer-reviewed]
  English, Scientific journal
• Effective Repair of Dural Tear Using Bioabsorbable Sheet with Fibrin Glue
  Akira Iwata; Masahiko Takahata; Ken Kadoya; Hideaki Sudo; Terufumi Kokabu; Katsuhisa Yamada; Norimasa Iwasaki
  Spine, 42, 18, 1362, 1366, Lippincott Williams and Wilkins, 15 Sep. 2017, [Peer-reviewed]
  English, Scientific journal
• Compound Heterozygosity for Null Mutations and a Common Hypomorphic Risk Haplotype in TBX6 Causes Congenital Scoliosis
  Kazuki Takeda; Ikuyo Kou; Noriaki Kawakami; Aritoshi Iida; Masahiro Nakajima; Yoji Ogura; Eri Imagawa; Noriko Miyake; Naomichi Matsumoto; Yukuto Yasuhiko; Hideki Sudo; Toshiaki Kotani; Masaya Nakamura; Morio Matsumoto; Kota Watanabe; Shiro Ikegawa
  HUMAN MUTATION, 38, 3, 317, 323, Mar. 2017, [Peer-reviewed]
  English, Scientific journal
• Cervical myelopathy due to atraumatic odontoid fracture in patients with rheumatoid arthritis: A case series
  Masahiko Takahata; Kuniyoshi Abumi; Hideki Sudo; Ken Nagahama; Norimasa Iwasaki
  MODERN RHEUMATOLOGY, 27, 5, 901, 904, 2017, [Peer-reviewed]
  English, Scientific journal
• Effects of Multiple Intra-articular Injections of 0.5% Bupivacaine on Normal and Osteoarthritic Joints in Rats
  Koji Iwasaki; Hideki Sudo; Yasuhiko Kasahara; Katsuhisa Yamada; Takashi Ohnishi; Takeru Tsujimoto; Norimasa Iwasaki
  ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY, 32, 10, 2026, 2036, Oct. 2016, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Correlation analysis between change in thoracic kyphosis and multilevel facetectomy and screw density in main thoracic adolescent idiopathic scoliosis surgery
  Hideki Sudo; Yuichiro Abe; Terufumi Kokabu; Manabu Ito; Kuniyoshi Abumi; Yoichi M. Ito; Norimasa Iwasaki
  SPINE JOURNAL, 16, 9, 1049, 1054, Sep. 2016, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• In Vivo Mouse Intervertebral Disc Degeneration Model Based on a New Histological Classification
  Takashi Ohnishi; Hideki Sudo; Koji Iwasaki; Takeru Tsujimoto; Yoichi M. Ito; Norimasa Iwasaki
  PLOS ONE, 11, 8, e0160486, Aug. 2016, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Effects of Multilevel Facetectomy and Screw Density on Postoperative Changes in Spinal Rod Contour in Thoracic Adolescent Idiopathic Scoliosis Surgery
  Terufumi Kokabu; Hideki Sudo; Yuichiro Abe; Manabu Ito; Yoichi M. Ito; Norimasa Iwasaki
  PLOS ONE, 11, 8, e0161906, Aug. 2016, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Maintenance of spontaneous lumbar curve correction following thoracic fusion of main thoracic curves in adolescent idiopathic scoliosis
  H. S. Sudo; M. M. Mayer; K. K. Kaneda; S. Nunez-Pereira; S. Y. Shono; W. H. Hitzl; N. I. Iwasaki; H. K. Koller
  BONE & JOINT JOURNAL, 98B, 7, 997, 1002, Jul. 2016, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Short fusion strategy for thoracolumbar and lumbar adolescent idiopathic scoliosis using anterior dual-rod instrumentation.
  H. Sudo; K. Kaneda; Y. Shono; N. Iwasaki
  BONE & JOINT JOURNAL, 98B, 3, 402, 409, Mar. 2016, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Selection of the upper vertebra to be instrumented in the treatment of thoracolumbar and lumbar adolescent idiopathic scoliosis by anterior correction and fusion surgery using dual-rod instrumentation: a minimum 12-year follow-up study
  Hideki Sudo; Kiyoshi Kaneda; Yasuhiro Shono; Norimasa Iwasaki
  SPINE JOURNAL, 16, 3, 281, 287, Mar. 2016, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Surgical treatment of double thoracic adolescent idiopathic scoliosis with a rigid proximal thoracic curve
  Hideki Sudo; Yuichiro Abe; Kuniyoshi Abumi; Norimasa Iwasaki; Manabu Ito
  EUROPEAN SPINE JOURNAL, 25, 2, 569, 577, Feb. 2016, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• A Functional SNP in BNC2 Is Associated with Adolescent Idiopathic Scoliosis
  Yoji Ogura; Ikuyo Kou; Shigenori Miura; Atsushi Takahashi; Leilei Xu; Kazuki Takeda; Yohei Takahashi; Katsuki Kono; Noriaki Kawakami; Koki Uno; Manabu Ito; Shohei Minami; Ikuho Yonezawa; Haruhisa Yanagida; Hiroshi Taneichi; Zezhang Zhu; Taichi Tsuji; Teppei Suzuki; Hideki Sudo; Toshiaki Kotani; Kota Watanabe; Naobumi Hosogane; Eijiro Okada; Aritoshi Iida; Masahiro Nakajima; Akihiro Sudo; Kazuhiro Chiba; Yuji Hiraki; Yoshiaki Toyama; Yong Qiu; Chisa Shukunami; Yoichiro Kamatani; Michiaki Kubo; Mono Matsumoto; Shiro Ikegawal
  AMERICAN JOURNAL OF HUMAN GENETICS, 97, 2, 337, 342, Aug. 2015, [Peer-reviewed]
  English, Scientific journal
• 18F-FDG-PET/CT better localizes active spinal infection than MRI for successful minimally invasive surgery
  Masayuki Nakahara; Manabu Ito; Naoya Hattori; Keiichi Magota; Masahiko Takahata; Ken Nagahama; Hideki Sudo; Tamotsu Kamishima; Nagara Tamaki; Norimasa Iwasaki
  ACTA RADIOLOGICA, 56, 7, 829, 836, Jul. 2015, [Peer-reviewed]
  English, Scientific journal
• Right-convex thoracolumbar scoliosis
  Kei Kuroki; Hideki Sudo; Norimasa Iwasaki
  SPINE JOURNAL, 15, 5, 1154, 1155, May 2015, [Peer-reviewed], [Corresponding author]
  English
• A PAX1 enhancer locus is associated with susceptibility to idiopathic scoliosis in females
  Swarkar Sharma; Douglas Londono; Walter L. Eckalbar; Xiaochong Gao; Dongping Zhang; Kristen Mauldin; Ikuyo Kou; Atsushi Takahashi; Morio Matsumoto; Nobuhiro Kamiya; Karl K. Murphy; Reuel Cornelia; John A. Herring; Dennis Burns; Nadav Ahituv; Shiro Ikegawa; Derek Gordon; Carol A. Wise
  NATURE COMMUNICATIONS, 6, 6, 6452, Mar. 2015, [Peer-reviewed]
  English, Scientific journal
• Scoliosis corrective force estimation from the implanted rod deformation using 3D-FEM analysis
  Yuichiro Abe; Manabu Ito; Kuniyoshi Abumi; Hideki Sudo; Remel Salmingo; Shigeru Tadano
  Scoliosis, 10, Suppl 2, S2, BioMed Central Ltd., 11 Feb. 2015, [Peer-reviewed]
  English, Scientific journal
• Subaxial Sagittal Alignment After Atlantoaxial Fixation Techniques
  Shigeki Oshima; Hideki Sudo; Manabu Ito; Kuniyoshi Abumi
  JOURNAL OF SPINAL DISORDERS & TECHNIQUES, 28, 1, E49, E55, Feb. 2015, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Effects of Single Injection of Local Anesthetic Agents on Intervertebral Disc Degeneration: Ex Vivo and Long-Term In Vivo Experimental Study
  Koji Iwasaki; Hideki Sudo; Katsuhisa Yamada; Hideaki Higashi; Takashi Ohnishi; Takeru Tsujimoto; Norimasa Iwasaki
  PLOS ONE, 9, 10, e109851, Oct. 2014, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Surgical Treatment of Lenke 1 Thoracic Adolescent Idiopathic Scoliosis With Maintenance of Kyphosis Using the Simultaneous Double-Rod Rotation Technique
  Hideki Sudo; Manabu Ito; Yuichiro Abe; Kuniyoshi Abumi; Masahiko Takahata; Ken Nagahama; Shigeto Hiratsuka; Kei Kuroki; Norimasa Iwasaki
  SPINE, 39, 14, 1163, 1169, Jun. 2014, [Peer-reviewed], [Lead author]
  English, Scientific journal
• A meta-analysis identifies adolescent idiopathic scoliosis association with LBX1 locus in multiple ethnic groups
  Douglas Londono; Ikuyo Kou; Todd A. Johnson; Swarkar Sharma; Yoji Ogura; Tatsuhiko Tsunoda; Atsushi Takahashi; Morio Matsumoto; John A. Herring; Tsz-Ping Lam; Xingyan Wang; Elisa M. S. Tam; You-Qiang Song; Yan-Hui Fan; Danny Chan; Kathryn S. E. Cheah; Xusheng Qiu; Hua Jiang; Dongsheng Huang; Peiqiang Su; Pak Sham; Kenneth M. C. Cheung; Keith D. K. Luk; Derek Gordon; Yong Qiu; Jack Cheng; Nelson Tang; Shiro Ikegawa; Carol A. Wise
  JOURNAL OF MEDICAL GENETICS, 51, 6, 401, 406, Jun. 2014, [Peer-reviewed]
  English, Scientific journal
• Fungal Spinal Infection Treated with Percutaneous Posterolateral Endoscopic Surgery
  Akira Iwata; Manabu Ito; Kuniyoshi Abumi; Hideki Sudo; Yoshihisa Kotani; Yasuhiro Shono; Akio Minami
  JOURNAL OF NEUROLOGICAL SURGERY PART A-CENTRAL EUROPEAN NEUROSURGERY, 75, 3, 170, 176, May 2014, [Peer-reviewed]
  English, Scientific journal
• Anomalous vertebral and posterior communicating arteries as a risk factor in instrumentation of the posterior cervical spine
  K. Nagahama; H. Sudo; K. Abumi; M. Ito; M. Takahata; S. Hiratsuka; K. Kuroki; N. Iwasaki
  BONE & JOINT JOURNAL, 96B, 4, 535, 540, Apr. 2014, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Cytotoxic Effects of the Radiocontrast Agent Iotrolan and Anesthetic Agents Bupivacaine and Lidocaine in Three-Dimensional Cultures of Human Intervertebral Disc Nucleus Pulposus Cells: Identification of the Apoptotic Pathways
  Koji Iwasaki; Hideki Sudo; Katsuhisa Yamada; Manabu Ito; Norimasa Iwasaki
  PLOS ONE, 9, 3, e92442, Mar. 2014, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Caspase 3 Silencing Inhibits Biomechanical Overload-Induced Intervertebral Disk Degeneration
  Katsuhisa Yamada; Hideki Sudo; Koji Iwasaki; Naoki Sasaki; Hideaki Higashi; Yusuke Kameda; Manabu Ito; Masahiko Takahata; Kuniyoshi Abumi; Akio Minami; Norimasa Iwasaki
  AMERICAN JOURNAL OF PATHOLOGY, 184, 3, 753, 764, Mar. 2014, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Cervical spondylotic myelopathy with subacute combined degeneration
  Takuji Miyazaki; Hideki Sudo; Shigeto Hiratsuka; Norimasa Iwasaki
  SPINE JOURNAL, 14, 2, 381, 382, Feb. 2014, [Peer-reviewed], [Corresponding author]
  English
• Anomalous vertebral and posterior communicating arteries as a risk factor in instrumentation of the posterior cervical spine
  K. Nagahama; H. Sudo; K. Abumi; M. Ito; M. Takahata; S. Hiratsuka; K. Kuroki; N. Iwasaki
  Bone and Joint Journal, 96, 4, 535, 540, British Editorial Society of Bone and Joint Surgery, 2014, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Anterior decompression and strut graft versus posterior decompression and pedicle screw fixation with vertebroplasty for osteoporotic thoracolumbar vertebral collapse with neurologic deficits
  Hideki Sudo; Manabu Ito; Kiyoshi Kaneda; Kuniyoshi Abumi; Yoshihisa Kotani; Ken Nagahama; Akio Minami; Norimasa Iwasaki
  Spine Journal, 13, 12, 1726, 1732, 12, 01 Dec. 2013, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Primary spinal marginal zone lymphoma relapse at a different spinal level after remission of the primary lesion.
  Hojo Y; Ito M; Abumi K; Sudo H; Takahata M
  Global spine journal, 3, 4, 261, 264, 4, Dec. 2013, [Peer-reviewed]
• Severe fixed cervical kyphosis treated with circumferential osteotomy and pedicle screw fixation using an anterior-posterior-anterior surgical sequence
  Hiroyuki Yoshihara; Kuniyoshi Abumi; Manabu Ito; Yoshihisa Kotani; Hideki Sudo; Masahiko Takahata
  World Neurosurgery, 80, 5, 654, e21, Nov. 2013, [Peer-reviewed]
  English
• Identification of a Susceptibility Locus for Severe Adolescent Idiopathic Scoliosis on Chromosome 17q24.3
  Atsushi Miyake; Ikuyo Kou; Yohei Takahashi; Todd A. Johnson; Yoji Ogura; Jin Dai; Xusheng Qiu; Atsushi Takahashi; Hua Jiang; Huang Yan; Katsuki Kono; Noriaki Kawakami; Koki Uno; Manabu Ito; Shohei Minami; Haruhisa Yanagida; Hiroshi Taneichi; Naoya Hosono; Taichi Tsuji; Teppei Suzuki; Hideki Sudo; Toshiaki Kotani; Ikuho Yonezawa; Michiaki Kubo; Tatsuhiko Tsunoda; Kota Watanabe; Kazuhiro Chiba; Yoshiaki Toyama; Yong Qiu; Morio Matsumoto; Shiro Ikegawa
  PLOS ONE, 8, 9, e72802, Sep. 2013, [Peer-reviewed]
  English, Scientific journal
• A replication study for association of 53 single nucleotide polymorphisms in a scoliosis prognostic test with progression of adolescent idiopathic scoliosis in Japanese
  Yoji Ogura; Yohei Takahashi; Ikuyo Kou; Masahiro Nakajima; Katsuki Kono; Noriaki Kawakami; Koki Uno; Manabu Ito; Shohei Minami; Haruhisa Yanagida; Hiroshi Taneichi; Ikuho Yonezawa; Taichi Tsuji; Teppei Suzuki; Hideki Sudo; Toshiaki Kotani; Kota Watanabe; Kazuhiro Chiba; Yoshiaki Toyama; Morio Matsumoto; Shiro Ikegawa
  Spine, 38, 16, 1375, 1379, 16, 15 Jul. 2013, [Peer-reviewed]
  English, Scientific journal
• Genetic variants in GPR126 are associated with adolescent idiopathic scoliosis
  Ikuyo Kou; Yohei Takahashi; Todd A. Johnson; Atsushi Takahashi; Long Guo; Jin Dai; Xusheng Qiu; Swarkar Sharma; Aki Takimoto; Yoji Ogura; Hua Jiang; Huang Yan; Katsuki Kono; Noriaki Kawakami; Koki Uno; Manabu Ito; Shohei Minami; Haruhisa Yanagida; Hiroshi Taneichi; Naoya Hosono; Taichi Tsuji; Teppei Suzuki; Hideki Sudo; Toshiaki Kotani; Ikuho Yonezawa; Douglas Londono; Derek Gordon; John A. Herring; Kota Watanabe; Kazuhiro Chiba; Naoyuki Kamatani; Qing Jiang; Yuji Hiraki; Michiaki Kubo; Yoshiaki Toyama; Tatsuhiko Tsunoda; Carol A. Wise; Yong Qiu; Chisa Shukunami; Morio Matsumoto; Shiro Ikegawa
  Nature Genetics, 45, 6, 676, 679, 6, Jun. 2013, [Peer-reviewed]
  English, Scientific journal
• Genetic variants in GPR126 are associated with adolescent idiopathic scoliosis
  Ikuyo Kou; Yohei Takahashi; Todd A. Johnson; Atsushi Takahashi; Long Guo; Jin Dai; Xusheng Qiu; Swarkar Sharma; Aki Takimoto; Yoji Ogura; Hua Jiang; Huang Yan; Katsuki Kono; Noriaki Kawakami; Koki Uno; Manabu Ito; Shohei Minami; Haruhisa Yanagida; Hiroshi Taneichi; Naoya Hosono; Taichi Tsuji; Teppei Suzuki; Hideki Sudo; Toshiaki Kotani; Ikuho Yonezawa; Douglas Londono; Derek Gordon; John A. Herring; Kota Watanabe; Kazuhiro Chiba; Naoyuki Kamatani; Qing Jiang; Yuji Hiraki; Michiaki Kubo; Yoshiaki Toyama; Tatsuhiko Tsunoda; Carol A. Wise; Yong Qiu; Chisa Shukunami; Morio Matsumoto; Shiro Ikegawa
  Nature Genetics, 45, 6, 676, 679, Jun. 2013
  English, Scientific journal
• Long-term Outcomes of Anterior Spinal Fusion for Treating Thoracic Adolescent Idiopathic Scoliosis Curves Average 15-Year Follow-up Analysis
  Hideki Sudo; Manabu Ito; Kiyoshi Kaneda; Yasuhiro Shono; Masahiko Takahata; Kuniyoshi Abumi
  SPINE, 38, 10, 819, 826, May 2013, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Long-term outcomes of anterior dual-rod instrumentation for thoracolumbar and lumbar curves in adolescent idiopathic scoliosis: A twelve to twenty-three-year follow-up study
  Hideki Sudo; Manabu Ito; Kiyoshi Kaneda; Yasuhiro Shono; Kuniyoshi Abumi
  Journal of Bone and Joint Surgery - Series A, 95, 8, e491, e498, Journal of Bone and Joint Surgery Inc., 17 Apr. 2013, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• A Replication Study for Association of 5 Single Nucleotide Polymorphisms With Curve Progression of Adolescent Idiopathic Scoliosis in Japanese Patients
  Yoji Ogura; Yohei Takahashi; Ikuyo Kou; Masahiro Nakajima; Katsuki Kono; Noriaki Kawakami; Koki Uno; Manabu Ito; Shohei Minami; Haruhisa Yanagida; Hiroshi Taneichi; Ikuho Yonezawa; Taichi Tsuji; Teppei Suzuki; Hideki Sudo; Toshiaki Kotani; Kota Watanabe; Kazuhiro Chiba; Yoshiaki Toyama; Morio Matsumoto; Shiro Ikegawa
  SPINE, 38, 7, 571, 575, Apr. 2013, [Peer-reviewed]
  English, Scientific journal
• Global Identification of Genes Related to Nutrient Deficiency in Intervertebral Disc Cells in an Experimental Nutrient Deprivation Model
  Hideki Sudo; Katsuhisa Yamada; Koji Iwasaki; Hideaki Higashi; Manabu Ito; Akio Minami; Norimasa Iwasaki
  PLOS ONE, 8, 3, e58806, Mar. 2013, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Cervical myelopathy resulting from combined ossification of the ligamentum flavum and posterior longitudinal ligament: Report of two cases and literature review
  Yoshihisa Kotani; Masahiko Takahata; Kuniyoshi Abumi; Manabu Ito; Hideki Sudo; Akio Minami
  Spine Journal, 13, 1, -e6, 6, 1, Jan. 2013, [Peer-reviewed]
  English, Scientific journal
• Impact of deep extensor muscle-preserving approach on clinical outcome of laminoplasty for cervical spondylotic myelopathy: comparative cohort study
  Yoshihisa Kotani; Kuniyoshi Abumi; Manabu Ito; Hideki Sudo; Masahiko Takahata; Ken Nagahama; Akira Iwata; Akio Minami
  EUROPEAN SPINE JOURNAL, 21, 8, 1536, 1544, Aug. 2012, [Peer-reviewed]
  English, Scientific journal
• A retrospective study of congenital osseous anomalies at the craniocervical junction treated by occipitocervical plate-rod systems
  Xianjun Ding; Kuniyoshi Abumi; Manabu Ito; Hideki Sudo; Masahiko Takahata; Ken Nagahama; Akira Iwata
  EUROPEAN SPINE JOURNAL, 21, 8, 1580, 1589, Aug. 2012, [Peer-reviewed]
  English, Scientific journal
• Post-traumatic lumbar subdural hematoma
  Hideki Sudo; Kuniyoshi Abumi; Yusuke Menjo; Manabu Ito
  SPINE JOURNAL, 12, 8, 714, 715, Aug. 2012, [Peer-reviewed], [Lead author, Corresponding author]
  English
• Mid-term clinical results of minimally invasive decompression and posterolateral fusion with percutaneous pedicle screws versus conventional approach for degenerative spondylolisthesis with spinal stenosis
  Yoshihisa Kotani; Kuniyoshi Abumi; Manabu Ito; Hideki Sudo; Yuichiro Abe; Akio Minami
  EUROPEAN SPINE JOURNAL, 21, 6, 1171, 1177, Jun. 2012, [Peer-reviewed]
  English, Scientific journal
• Spinal cord compression by hematoma in the cervical ligamentum flavum: a case report
  Yoshihisa Kotani; Hideki Sudo; Kuniyoshi Abumi; Manabu Ito; Shinji Matsubara; Akio Minami
  SPINE JOURNAL, 12, 4, E18, E21, Apr. 2012, [Peer-reviewed]
  English, Scientific journal
• Reconstruction of the Subaxial Cervical Spine Using Pedicle Screw Instrumentation
  Kuniyoshi Abumi; Manabu Ito; Hideki Sudo
  SPINE, 37, 5, E349, E356, Mar. 2012, [Peer-reviewed], [Last author]
  English, Scientific journal
• Cervical angina because of ossification of the posterior longitudinal ligament
  Hideki Sudo; Reo Goto
  SPINE JOURNAL, 12, 2, 169, 169, Feb. 2012, [Peer-reviewed], [Lead author, Corresponding author]
  English
• A genome-wide association study identifies common variants near LBX1 associated with adolescent idiopathic scoliosis
  Yohei Takahashi; Ikuyo Kou; Atsushi Takahashi; Todd A. Johnson; Katsuki Kono; Noriaki Kawakami; Koki Uno; Manabu Ito; Shohei Minami; Haruhisa Yanagida; Hiroshi Taneichi; Taichi Tsuji; Teppei Suzuki; Hideki Sudo; Toshiaki Kotani; Kota Watanabe; Kazuhiro Chiba; Naoya Hosono; Naoyuki Kamatani; Tatsuhiko Tsunoda; Yoshiaki Toyama; Michiaki Kubo; Morio Matsumoto; Shiro Ikegawa
  NATURE GENETICS, 43, 12, 1237, U96, Dec. 2011, [Peer-reviewed]
  English, Scientific journal
• A novel cost-effective computer-assisted imaging technology for accurate placement of thoracic pedicle screws
  Yuichiro Abe; Manabu Ito; Kuniyoshi Abumi; Yoshihisa Kotani; Hideki Sudo; Akio Minami
  JOURNAL OF NEUROSURGERY-SPINE, 15, 5, 479, 485, Nov. 2011, [Peer-reviewed]
  English, Scientific journal
• Lack of Association between Adolescent Idiopathic Scoliosis and Previously Reported Single Nucleotide Polymorphisms in MATN1, MTNR1B, TPH1, and IGF1 in a Japanese Population
  Yohei Takahashi; Morio Matsumoto; Tatsuki Karasugi; Kota Watanabe; Kazuhiro Chiba; Noriaki Kawakami; Taichi Tsuji; Koki Uno; Teppei Suzuki; Manabu Ito; Hideki Sudo; Shohei Minami; Toshiaki Kotani; Katsuki Kono; Haruhisa Yanagida; Hiroshi Taneichi; Atsushi Takahashi; Yoshiaki Toyama; Shiro Ikegawa
  JOURNAL OF ORTHOPAEDIC RESEARCH, 29, 7, 1055, 1058, Jul. 2011, [Peer-reviewed]
  English, Scientific journal
• A late neurological complication following posterior correction surgery of severe cervical kyphosis
  Yoshihiro Hojo; Manabu Ito; Kuniyoshi Abumi; Yoshihisa Kotani; Hideki Sudo; Masahiko Takahata; Akio Minami
  EUROPEAN SPINE JOURNAL, 20, 6, 890, 898, Jun. 2011, [Peer-reviewed]
  English, Scientific journal
• Caspase 3 as a Therapeutic Target for Regulation of Intervertebral Disc Degeneration in Rabbits
  Hideki Sudo; Akio Minami
  ARTHRITIS AND RHEUMATISM, 63, 6, 1648, 1657, Jun. 2011, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Replication Study of the Association between Adolescent Idiopathic Scoliosis and Two Estrogen Receptor Genes
  Yohei Takahashi; Mono Matsumoto; Tatsuki Karasugi; Kota Watanabe; Kazuhiro Chiba; Noriaki Kawakami; Taichi Tsuji; Koki Uno; Teppei Suzuki; Manabu Ito; Hideki Sudo; Shohei Minami; Toshiaki Kotani; Katsuki Kono; Haruhisa Yanagida; Hiroshi Taneichi; Atsushi Takahashi; Yoshiaki Toyama; Shiro Ikegawa
  JOURNAL OF ORTHOPAEDIC RESEARCH, 29, 6, 834, 837, Jun. 2011, [Peer-reviewed]
  English, Scientific journal
• Regulation of Apoptosis in Nucleus Pulposus Cells by Optimized Exogenous Bcl-2 Overexpression
  Hideki Sudo; Akio Minami
  JOURNAL OF ORTHOPAEDIC RESEARCH, 28, 12, 1608, 1613, Dec. 2010, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• A widespread echinococcosis of the spine
  H. Sudo; A. Minami
  JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, 81, 8, 892, 892, Aug. 2010, [Peer-reviewed], [Lead author, Corresponding author]
  English
• One-stage posterior instrumentation surgery for the treatment of osteoporotic vertebral collapse with neurological deficits
  Hideki Sudo; Manabu Ito; Kuniyoshi Abumi; Yoshihisa Kotani; Masahiko Takahata; Yoshihiro Hojo; Akio Minami
  EUROPEAN SPINE JOURNAL, 19, 6, 907, 915, Jun. 2010, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Simultaneous double-rod rotation technique in posterior instrumentation surgery for correction of adolescent idiopathic scoliosis Technical note
  Manabu Ito; Kuniyoshi Abumi; Yoshihisa Kotani; Masahiko Takahata; Hideki Sudo; Yoshihiro Hojo; Akio Minami
  JOURNAL OF NEUROSURGERY-SPINE, 12, 3, 293, 300, Mar. 2010, [Peer-reviewed]
  English, Scientific journal
• Spinal Cord Compression by Ligamentum Flavum Hematoma in the Thoracic Spine
  Hideki Sudo; Kuniyoshi Abumi; Manabu Ito; Yoshihisa Kotani; Masahiko Takahata; Yoshihiro Hojo; Masatoshi Sanda; Akio Minami
  SPINE, 34, 25, E942, E944, Dec. 2009, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Minimally Invasive Surgical Treatment for Tuberculous Spondylodiscitis
  M. Ito; H. Sudo; K. Abumi; Y. Kotani; M. Takahata; M. Fujita; A. Minami
  MINIMALLY INVASIVE NEUROSURGERY, 52, 5-6, 250, 253, Oct. 2009, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Minimum 2-year outcome of cervical laminoplasty with deep extensor muscle-preserving approach: impact on cervical spine function and quality of life
  Yoshihisa Kotani; Kuniyoshi Abumi; Manabu Ito; Hideki Sudo; Masahiko Takahata; Shigeki Ohshima; Yoshihiro Hojo; Akio Minami
  EUROPEAN SPINE JOURNAL, 18, 5, 663, 671, May 2009, [Peer-reviewed]
  English, Scientific journal
• Two cases of postoperative MRSA infection after a transforaminal lumbar interbody fusion
  Katsuhisa Yamada; Yoshihisa Kotani; Manabu Ito; Kuniyoshi Abumi; Hideki Sudo; Masahiko Takahata; Shigeki Oshima; Akio Minami
  Hokkaido Journal of Orthopaedics and Traumatology, 50, 2, 220, 223, Mar. 2009
  Japanese, Scientific journal
• Clinical results and complications of circumferential spinal cord decompression through a single posterior approach for thoracic myelopathy caused by ossification of posterior longitudinal ligament
  Masahiko Takahata; Manabu Ito; Kuniyoshi Abumi; Yoshihisa Kotani; Hideki Sudo; Akio Minami
  SPINE, 33, 11, 1199, 1208, May 2008, [Peer-reviewed]
  English, Scientific journal
• Comparison of novel ultra-high molecular weight polyethylene tape versus conventional metal wire for sublaminar segmental fixation in the treatment of adolescent idiopathic scoliosis
  Masahiko Takahata; Manabu Ito; Kuniyoshi Aburni; Yoshihisa Kotani; Hideki Sudo; Shigeki Ohshima; Akio Minami
  JOURNAL OF SPINAL DISORDERS & TECHNIQUES, 20, 6, 449, 455, Aug. 2007, [Peer-reviewed]
  English, Scientific journal
• Accuracy analysis of pedicle screw placement in posterior scoliosis surgery - Comparison between conventional fluoroscopic and computer-assisted technique
  Yoshihisa Kotani; Kuniyoshi Abumi; Manabu Ito; Masahiko Takahata; Hideki Sudo; Shigeki Ohshima; Akio Minami
  SPINE, 32, 14, 1543, 1550, Jun. 2007, [Peer-reviewed]
  English, Scientific journal
• Long-term follow up of surgical outcomes in patients with cervical disorders undergoing hemodialysis
  Hideki Sudo; Manabu Ito; Kuniyoshi Abumi; Yoshihisa Kotani; Tatsuto Takeuchi; Keigo Yasui; Akio Minami
  JOURNAL OF NEUROSURGERY-SPINE, 5, 4, 313, 319, Oct. 2006, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Biomechanical study on the effect of five different lumbar reconstruction techniques on adjacent-level intradiscal pressure and lamina strain
  Hideki Sudo; Itaru Oda; Kuniyoshi Abumi; Manabu Ito; Yoshihisa Kotani; Akio Minami
  JOURNAL OF NEUROSURGERY-SPINE, 5, 2, 150, 155, Aug. 2006, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Secondary medulla oblongata involvement following middle cervical spinal cord injury associated with latent traumatic instability in a patient with ossification of the posterior longitudinal ligament
  H Sudo; H Taneichi; K Kaneda
  SPINAL CORD, 44, 2, 126, 129, Feb. 2006, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Burkholderia pickettii spondylitis
  H Sudo; Y Hisada; M Ito; H Kotaki; A Minami
  SPINAL CORD, 43, 8, 499, 502, Aug. 2005, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• In vitro biomechanical effects of reconstruction on adjacent motion segment: comparison of aligned/kyphotic posterolateral fusion with aligned posterior lumbar interbody fusion/posterolateral fusion
  H Sudo; Oda, I; K Abumi; M Ito; Y Kotani; Y Hojo; A Minami
  JOURNAL OF NEUROSURGERY, 99, 2, 221, 228, Sep. 2003, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Types of spinal instability that require interbody support in posterior lumbar reconstruction - An in vitro biomechanical investigation
  Oda, I; K Abumi; BS Yu; H Sudo; A Minami
  SPINE, 28, 14, 1573, 1580, Jul. 2003, [Peer-reviewed]
  English, Scientific journal
• A moving middle finger
  H Sudo; M Ito; A Minami
  LANCET, 361, 9376, 2202, 2202, Jun. 2003, [Peer-reviewed], [Lead author, Corresponding author]
  English
• NF-kappa B-dependent induction of cyclin D1 by retinoblastoma protein (pRB) family proteins and tumor-derived pRB mutants
  T Takebayashi; H Higashi; H Sudo; H Ozawa; E Suzuki; O Shirado; H Katoh; M Hatakeyama
  JOURNAL OF BIOLOGICAL CHEMISTRY, 278, 17, 14897, 14905, Apr. 2003, [Peer-reviewed]
  English, Scientific journal
• Spinal cord compression by multistrand cables after solid posterior atlantoaxial fusion - Report of three cases
  H Sudo; K Abumi; M Ito; Y Kotani; A Minami
  JOURNAL OF NEUROSURGERY, 97, 3, 359, 361, Oct. 2002, [Peer-reviewed], [Lead author]
  English, Scientific journal

• 解剖学的4D矯正法が思春期特発性側弯症症例の片脚立位における姿勢制御能力に及ぼす影響
  大須賀聡; 須藤英毅; 山田勝久; 舘弘之; 福島瑛; 渡邊謙太郎; 千徳風真; 岩崎倫政; 遠山晴一, 日本整形外科学会雑誌(CD-ROM), 99, 3, 2025
• Automated Diagnosis of Lumbar Spinal Canal Stenosis Using AI
  鈴木久崇; 小甲晃史; 山田勝久; 須藤英毅, 脊椎脊髄ジャーナル, 38, 4, 2025
• Impact of Visceral Fat Obesity on the Development of Spinal Ligament Ossification-Analysis of Health Examination Data of 249 Patients-
  三浦宗也; 遠藤努; 小池良直; 藤田諒; 山田勝久; 大西貴士; 筌場大介; 舘弘之; 金山雅弘; 深田翔太郎; 須藤英毅; 岩崎倫政; 高畑雅彦, Journal of Spine Research (Web), 15, 3, 2024
• Visceral Fat is Strongly Associated with the Severity of Spinal Ligament Ossification
  遠藤努; 遠藤努; 三浦宗也; 小池良直; 藤田諒; 山田勝久; 大西貴士; 筌場大介; 舘弘之; 金山雅弘; 深田翔太朗; 須藤英毅; 岩崎倫政; 高畑雅彦, Journal of Spine Research (Web), 15, 3, 2024
• Risk factors for ossification of the posterior longitudinal ligament leading to lumbar spine surgery
  小池良直; 遠藤努; 遠藤努; 高畑雅彦; 金山雅弘; 山田勝久; 大西貴士; 筌場大介; 舘弘之; 須藤英毅; 石井美咲; 岩崎倫政, Journal of Spine Research (Web), 15, 3, 2024
• Prediction of cervical spondylosis classification using deep learning with convolutional neural network
  舘弘之; 小甲晃史; 山田勝久; 筌場大介; 大西貴史; 遠藤努; 須藤英毅; 岩崎倫政, 日本関節病学会誌(Web), 43, 2, 2024
• AI作成3D腰神経像を用いた経椎間孔全内視鏡下ヘルニア切除術シミュレーションの有用性-上関節突起部分切除例を含めた検討-
  筌場大介; 山田勝久; 山田勝久; 長濱賢; 安倍雄一郎; 日向寺義則; 舘弘之; 遠藤努; 大西貴士; 須藤英毅; 高畑雅彦; 岩崎倫政, 日本整形外科学会雑誌, 98, 2, 2024
• AI作成三次元腰神経MRI・腰仙椎CT融合画像を用いた全内視鏡下椎間板ヘルニア摘出術経椎弓間法シミュレーションの有用性
  筌場大介; 山田勝久; 山田勝久; 長濱賢; 安倍雄一郎; 日向寺義則; 舘弘之; 遠藤努; 大西貴士; 須藤英毅; 高畑雅彦; 岩崎倫政, 日本整形外科学会雑誌, 98, 2, 2024
• 非アルコール性脂肪肝(NAFLD)は後縦靭帯骨化症と強く関連する
  深田翔太郎; 金山雅弘; 遠藤努; 小池良直; 藤田諒; 山田勝久; 大西貴士; 筌場大介; 須藤英毅; 岩崎倫政; 高畑雅彦, 北海道整形災害外科学会, 143rd, 2024
• 全内視鏡下脊椎手術における経椎間孔アプローチのOutside-in法を安全かつ容易に行うためのDuckbill release法
  筌場大介; 山田勝久; 遠藤努; 大西貴士; 舘弘之; 須藤英毅; 岩崎倫政; 長濱賢; 安倍雄一郎, 北海道整形災害外科学会, 143rd, 2024
• Research of the surgical intervention for metastatic spinal tumors after radiotherapy
  岩田玲; 高畑雅彦; 安井啓悟; 伊東学; 楫野知道; 奥村潤一郎; 放生憲博; 平塚重人; 原谷健太郎; 須藤英毅; 松岡正剛; 山田勝久; 清水寛和; 岩崎倫政; 平賀博明, Journal of Spine Research (Web), 15, 3, 2024
• Regenerative therapy of intervertebral disc using alginate-based bioabsorbable biomaterials combined with bone marrow aspirate concentrate.
  筌場大介; 石川蓉子; 山田勝久; タ キンキン; 大西貴士; 遠藤努; 舘弘之; 高畑雅彦; 岩崎倫政; 須藤英毅, Journal of Spine Research (Web), 15, 3, 2024
• Non-alcoholic fatty liver disease (NAFLD) is strongly associated with posterior longitudinal ligament ossification
  深田翔太郎; 遠藤努; 遠藤努; 小池良直; 藤田諒; 山田勝久; 大西貴士; 筌場大介; 舘弘之; 金山雅弘; 須藤英毅; 橋本友幸; 岩崎倫政; 高畑雅彦, Journal of Spine Research (Web), 15, 3, 2024
• Simulation for full-endoscopic interlaminar lumbar discectomy using 3D lumbar nerve MRI images created automatically with AI
  山田勝久; 山田勝久; 長濱賢; 筌場大介; 安倍雄一郎; 日向寺義則; 舘弘之; 遠藤努; 大西貴士; 高畑雅彦; 須藤英毅; 岩崎倫政, Journal of Spine Research (Web), 15, 3, 2024
• Preoperative simulation of Full-endoscopic discectomy using 3D-MRI/CT fusion images at lumbosacral level-including outside-in technique-
  山田勝久; 山田勝久; 長濱賢; 筌場大介; 安倍雄一郎; 日向寺義則; 舘弘之; 遠藤努; 大西貴士; 高畑雅彦; 須藤英毅; 岩崎倫政, Journal of Spine Research (Web), 15, 3, 2024
• 胸腰椎化膿性椎体椎間板炎に対する全内視鏡下掻爬洗浄術の適応とその限界-大きい膿瘍腔は予後不良因子である-
  山田勝久; 長濱賢; 長濱賢; 遠藤努; 筌場大介; 大西貴士; 舘弘之; 長谷川裕一; 須藤英毅; 岩崎倫政; 伊東学, 日本低侵襲脊椎外科学会学術集会プログラム・抄録集, 27th, 2024
• 全内視鏡下脊椎手術における経椎間孔アプローチのOutside-in法を安全かつ容易に行うためのDuckbill release法
  山田勝久; 筌場大介; 長濱賢; 安倍雄一郎; 遠藤努; 大西貴士; 舘弘之; 須藤英毅; 岩崎倫政, 日本低侵襲脊椎外科学会学術集会プログラム・抄録集, 27th, 2024
• 腰椎変性すべり症における経椎間孔アプローチ全内視鏡下固定術に基づく安全領域の三次元解析-3D-MRI/CT融合画像を用いて-
  山田勝久; 長濱賢; 安倍雄一郎; 日向寺義則; 筌場大介; 舘弘之; 長谷川裕一; 遠藤努; 大西貴士; 須藤英毅; 岩崎倫政, 日本脊椎インストゥルメンテーション学会抄録集, 33rd, 2024
• AI作成3D腰神経MRI・腰仙椎CT融合画像を用いた経椎弓間法全内視鏡下椎間板ヘルニア摘出術シミュレーションの有用性
  山田勝久; 長濱賢; 安倍雄一郎; 日向寺義則; 筌場大介; 舘弘之; 遠藤努; 大西貴士; 須藤英毅; 岩崎倫政, 東日本整形災害外科学会雑誌(Web), 36, 3, 2024
• AI作成3D腰神経像を用いたL5/Sレベルの経椎間孔全内視鏡下ヘルニア切除術シミュレーションの有用性
  山田勝久; 長濱賢; 筌場大介; 安倍雄一郎; 日向寺義則; 舘弘之; 遠藤努; 大西貴士; 須藤英毅; 岩崎倫政, 東日本整形災害外科学会雑誌(Web), 36, 3, 2024
• 硬膜外血腫により遅発性麻痺を生じた高齢者の頚椎脱臼骨折の一例
  白井良樹; 白井良樹; 浦勝郎; 浦勝郎; 山田勝久; 筌場大介; 大西貴士; 遠藤努; 太田昌博; 須藤英毅; 岩崎倫政, 北海道整形災害外科学会雑誌(Web), 66, 1, 2024
• Drop fingerをきたしたC8神経根症の病態と手術治療成績
  泉泰純; 三浦宗也; 山田勝久; 高畑雅彦; 楫野知道; 岩田玲; 遠藤努; 久田雄一郎; 須藤英毅; 岩崎倫政, 北海道整形災害外科学会雑誌(Web), 66, 1, 2024
• Nucleic acids for regeneration of the intervertebral disc based on regenerative medicine using mesenchymal stem cell transplantation
  大西貴士; 宝満健太郎; 福島瑛; 須藤英毅; 山田勝久; 岩崎倫政, Journal of Spine Research (Web), 15, 3, 2024
• 経過中に腫瘍内出血を呈した脊髄円錐部上衣腫の1例
  JUNG Wooyoung; 山田勝久; 山田勝久; 高畑雅彦; 岩田玲; 遠藤努; 藤田諒; 須藤英毅; 岩崎倫政, 北海道整形災害外科学会雑誌(Web), 65, 1, 2023
• 有限要素解析による四次元シミュレーションソフトを用いた小児特発性側弯症に対する術前計画の検証
  舘弘之; 舘弘之; 加藤浩仁; 安倍雄一郎; 小甲晃史; 山田勝久; 岩崎倫政; 須藤英毅; 須藤英毅, 日本整形外科学会雑誌, 96, 2, 2022
• 有限要素解析による四次元シミュレーションソフトを用いた小児特発性側弯症に対する術前計画の検証
  舘弘之; 小甲晃史; 山田勝久; 岩崎倫政; 須藤英毅; 加藤浩仁; 舘弘之; 安倍雄一郎; 小甲晃史; 須藤英毅, 北海道整形災害外科学会, 141st, 2022
• Surgical outcome prediction using a four-dimensional planning simulation system with finite element analysis in AIS
  舘弘之; 舘弘之; 加藤浩仁; 安倍雄一郎; 小甲晃史; 山田勝久; 岩崎倫政; 須藤英毅; 須藤英毅, Journal of Spine Research (Web), 13, 3, 2022
• 脊柱変形疾患における革新的動的有限要素解析シミュレーションソフトの開発
  舘弘之; 舘弘之; 加藤浩仁; 安倍雄一郎; 小甲晃史; 小甲晃史; 山田勝久; 岩崎倫政; 須藤英毅; 須藤英毅, 日本整形外科学会雑誌, 95, 8, 2021
• ウサギ椎間板ヘルニアモデルにおける高純度硬化性ゲルの椎間板内炎症抑制効果についての検討
  浦勝郎; 筌場大介; 辻本武尊; 岩崎倫政; 須藤英毅, 北海道整形災害外科学会, 138th, 2020
• ウサギ髄核摘出モデルにおける高純度硬化性ゲルと自家骨髄濃縮液による組織修復効果
  筌場大介; 浦勝郎; 辻本武尊; 岩崎倫政; 須藤英毅, 北海道整形災害外科学会, 138th, 2020
• 骨髄由来間葉系幹細胞による椎間板細胞治療
  須藤英毅; 須藤英毅; 筌場大介; 岩崎倫政, 日本再生医療学会総会(Web), 19th, 2020
• 椎間板再生への挑戦 高純度硬化性ゲルによる椎間板再生
  辻本 武尊; 須藤 英毅; 東藤 正浩; 山田 勝久; 大西 貴士; 岩崎 倫政, 日本整形外科学会雑誌, 93, 8, S1595, S1595, Sep. 2019
  (公社)日本整形外科学会, Japanese
• 高純度硬化性ゲルと骨髄由来間葉系幹細胞による椎間板治療
  筌場 大介; 須藤 英毅; 浦 勝郎; 辻本 武尊; 岩崎 倫政, 日本整形外科学会雑誌, 93, 8, S1776, S1776, Sep. 2019
  (公社)日本整形外科学会, Japanese
• 骨粗鬆症性椎体骨折後の遅発性麻痺 アライメント変化による非骨折高位の腰部脊柱管狭窄の症状顕在化
  山口 哲也; 遠藤 努; 高畑 雅彦; 岩田 玲; 山田 勝久; 辻本 武尊; 須藤 英毅; 岩崎 倫政, 東日本整形災害外科学会雑誌, 31, 3, 259, 259, Aug. 2019
  東日本整形災害外科学会, Japanese
• 胸椎後縦靱帯骨化症にくも膜嚢胞を合併した胸髄症の2例
  小川 裕生; 高畑 雅彦; 岩田 玲; 山田 勝久; 遠藤 努; 辻本 武尊; 須藤 英毅; 岩崎 倫政, 東日本整形災害外科学会雑誌, 31, 3, 382, 382, Aug. 2019
  東日本整形災害外科学会, Japanese
• 胸椎後縦靱帯骨化症後方除圧固定術後20年来の慢性骨髄炎に対する治療経験
  山本 真弘; 辻本 武尊; 高畑 雅彦; 岩田 玲; 山田 勝久; 遠藤 努; 須藤 英毅; 岩崎 倫政, 東日本整形災害外科学会雑誌, 31, 3, 384, 384, Aug. 2019
  東日本整形災害外科学会, Japanese
• 単椎間経皮的椎弓根スクリュー固定と経皮的内視鏡下ドレナージ併用にて治癒し得た腰椎化膿性脊椎炎の2症例
  舘 弘之; 山田 勝久; 高畑 雅彦; 岩田 玲; 遠藤 努; 辻本 武尊; 須藤 英毅; 岩崎 倫政, 東日本整形災害外科学会雑誌, 31, 3, 413, 413, Aug. 2019
  東日本整形災害外科学会, Japanese
• 4D‐有限要素解析シミュレーションによる術前計画と積層造形技術を含む脊柱変形矯正用カスタムメイドインプラントの開発
  須藤英毅; 金井理; 小甲晃史; 安倍雄一郎; 岩崎倫政; 千葉晶彦, 別冊整形外科, 75, 218‐221, Apr. 2019
  Japanese, Introduction scientific journal
• 側弯検診システムの研究開発
  須藤英毅; 小甲晃史; 小甲晃史; 安倍雄一郎; 岩崎倫政; 金井理, 臨床整形外科, 54, 3, 306‐310, Mar. 2019
  Japanese, Introduction scientific journal
• 3次元スキャンと3次元対称性解析による脊柱側弯症高速・高感度検診システムの開発
  金井理; 須藤英毅, 医科学応用研究財団研究報告(CD-ROM), 36, 50‐57, Feb. 2019
  Japanese
• 椎間板ブロックが変性椎間板細胞・組織に与える影響
  浦勝郎; 須藤英毅; 筌場大介, Journal of Spine Research, 10, 3, 2019
• ウサギ椎間板ヘルニアモデルにおける高純度硬化性ゲルの椎間板内炎症抑制効果についての検討
  浦勝郎; 須藤英毅; 辻本武尊; 筌場大介; 岩崎倫政, 整形外科バイオマテリアル研究会プログラム・抄録集, 39th, 2019
• 高純度硬化性ゲルを併用した椎間板細胞治療における各種骨髄由来間葉系幹細胞の効果
  筌場大介; 浦勝郎; 辻本武尊; 岩崎倫政; 須藤英毅, 北海道整形災害外科学会, 137th, 2019
• TBX6遺伝子のmissense変異はTBX6の細胞内局在異常による機能の喪失により先天性側彎症を引き起こす
  大伴 直央; 武田 和樹; 小倉 洋二; 川上 紀明; 小谷 俊明; 須藤 英毅; 米澤 郁穂; 宇野 耕吉; 種市 洋; 渡辺 慶; 三宅 紀子; 南 昌平; 重松 英樹; 菅原 亮; 谷口 優樹; 中村 雅也; 松本 守雄; 渡邉 航太; 千葉 一裕; 池川 志郎, 日本整形外科学会雑誌, 92, 8, S1914, S1914, Aug. 2018
  (公社)日本整形外科学会, Japanese
• rs11190870が思春期特発性側彎症の重症度に関与するかの検討
  高橋 洋平; 小倉 洋二; 南 昌平; 川上 紀明; 宇野 耕吉; 伊東 学; 米澤 郁穂; 海渡 貴司; 柳田 晴久; 渡辺 慶; 種市 洋; 須藤 英毅; 播广谷 勝三; 谷口 優樹; 細金 直文; 小谷 俊明; 辻 太一; 鈴木 哲平; 武田 和樹; 岡田 英次朗; 藤田 順之; 八木 満; 中村 雅也; 松本 守雄; 渡邉 航太; 千葉 一裕; 池川 志郎, 日本整形外科学会雑誌, 92, 8, S1915, S1915, Aug. 2018
  (公社)日本整形外科学会, Japanese
• 整形外科領域におけるゲノム医療:現状と臨床への展開 先天性側彎症に対する遺伝子解析の現状
  武田 和樹; 黄 郁代; 小倉 洋二; 川上 紀明; 小谷 俊明; 須藤 英毅; 米澤 郁穂; 中村 雅也; 松本 守雄; 渡邉 航太; 千葉 一裕, 日本整形外科学会雑誌, 92, 8, S1853, S1853, Aug. 2018
  (公社)日本整形外科学会, Japanese
• 特発性側弯症手術に対して使用したロッドに対する形状類似分析手法の開発
  小甲晃史; 金井理; 安倍雄一郎; 岩崎倫政; 須藤英毅, Journal of Spine Research, 9, 3, 239, 25 Mar. 2018
  Japanese
• 北海道における整形外科基礎研究 基礎研究体制と概要 北海道大学整形外科
  角家 健; 近藤 英司; 高畑 雅彦; 須藤 英毅; 古川 潤一; 小野寺 智洋; アラー・テルカウイ; 岩崎 倫政, 北海道整形災害外科学会雑誌, 59, 2, 197, 204, Mar. 2018
  北海道整形災害外科学会, Japanese
• 重度側弯症に対して二期的手術を行った一例
  中村夢志郎; 須藤英毅; 安倍雄一郎; 岩田玲; 小甲晃史; 山田勝久; 高畑雅彦; 岩崎倫政, 北海道整形災害外科学会雑誌, 60, 1, 2018
• BNC2遺伝子は人種を超えて思春期特発性側弯症発症に関与する
  小倉洋二; 小倉洋二; 小倉洋二; 高橋洋平; 武田和樹; 武田和樹; 南昌平; 川上紀明; 宇野耕吉; 伊東学; 米澤郁穂; 海渡貴司; 柳田晴久; 渡辺慶; 種市洋; 須藤英毅; 播广谷勝三; 谷口優樹; 細金直文; 小谷俊明; 辻太一; 鈴木哲平; 岡田英次朗; 藤田順之; 八木満; 中村雅也; 松本守雄; 渡辺航太; 池川志郎, Journal of Spine Research, 9, 3, 2018
• RS11190870と思春期特発性側弯症の重症化に関する検討
  高橋洋平; 高橋洋平; 高橋洋平; 小倉洋二; 小倉洋二; 南昌平; 川上紀明; 宇野耕吉; 伊東学; 米澤郁穂; 海渡貴志; 柳田晴久; 渡辺慶; 種市洋; 須藤英毅; 播广谷勝三; 谷口優樹; 細金直文; 小谷俊明; 辻太一; 鈴木哲平; 武田和樹; 武田和樹; 岡田英次朗; 藤田順之; 八木満; 辻崇; 中村雅也; 松本守雄; 渡辺航太; 千葉一裕; 池川志郎, 日本側彎症学会演題抄録集, 52nd, 2018
• 先天性側弯症の新規原因遺伝子の探索
  武田和樹; 黄郁代; 小倉洋二; 小倉洋二; 川上紀明; 川上紀明; 小谷俊明; 小谷俊明; 須藤英毅; 須藤英毅; 米澤郁穂; 米澤郁穂; 宇野耕吉; 宇野耕吉; 種市洋; 種市洋; 渡辺慶; 渡辺慶; 三宅紀子; 南昌平; 南昌平; 重松英樹; 重松英樹; 菅原亮; 菅原亮; 谷口優樹; 谷口優樹; 中村雅也; 松本守雄; 渡辺航太; 池川志郎, 日本側彎症学会演題抄録集, 52nd, 2018
• RS12946942は多人種間で思春期特発性側弯症の重症化に関与する
  武田和樹; 武田和樹; 小倉洋二; 小倉洋二; 小倉洋二; 高橋洋平; 南昌平; 川上紀明; 宇野耕吉; 伊東学; 米澤郁穂; 海渡貴司; 柳田晴久; 渡辺慶; 種市洋; 須藤英毅; 播广谷勝三; 谷口優樹; 細金直文; 小谷俊明; 辻太一; 鈴木哲平; 岡田英次朗; 藤田順之; 八木満; 中村雅也; 松本守雄; 池川志郎; 渡辺航太, 日本側彎症学会演題抄録集, 52nd, 2018
• BNC2遺伝子は多人種で思春期特発性側弯症発症に関与する
  小倉洋二; 小倉洋二; 小倉洋二; 高橋洋平; 武田和樹; 武田和樹; 南昌平; 川上紀明; 宇野耕吉; 伊東学; 米澤郁穂; 海渡貴司; 柳田晴久; 渡辺慶; 種市洋; 須藤英毅; 播广谷勝三; 谷口優樹; 細金直文; 小谷俊明; 辻太一; 鈴木哲平; 岡田英次朗; 藤田順之; 八木満; 中村雅也; 松本守雄; 渡邉航太; 池川志郎, 日本整形外科学会雑誌, 92, 8, 2018
• 腰椎後方椎体間固定術後に生じた手術部位感染の治療経験
  原谷健太郎; 山田勝久; 小甲晃史; 高畑雅彦; 岩田玲; 筌場大介; 須藤英毅, 北海道整形災害外科学会雑誌, 59, 2, 2018
• 椎間板ブロックが変性椎間板細胞・組織に与える影響
  浦勝郎; 須藤英毅; 筌場大介; 辻本武尊; 岩崎倫政, 日本整形外科学会雑誌, 92, 8, 2018
• 医工連携による椎間板再生治療用組織修復材の開発
  須藤英毅; 辻本武尊; 東藤正浩; 筌場大介; 浦勝郎; 岩崎倫政, 日本整形外科学会雑誌, 92, 8, 2018
• 思春期特発性側弯症におけるロッド曲線形状の類似分析手法の開発
  小甲晃史; 金井理; 安倍雄一郎; 岩崎倫政; 須藤英毅, 北海道整形災害外科学会, 134th, 41, 2018
  Japanese
• 特発性側弯症手術に対して使用したロッドに対する形状類似分析手法の開発
  小甲晃史; 金井理; 安倍雄一郎; 岩崎倫政; 須藤英毅, 北海道整形災害外科学会, 135th, 28, 2018
  Japanese
• 3Dスキャナと非対称性解析に基づく脊柱側弯症早期検診システムの開発
  金井理; 須藤英毅; 小甲晃史; 長枝浩; 林隆行; 太田初, 日本設計工学会北海道支部研究発表論文集, 2018, 2, 13‐16, 2018
  Japanese
• 医工連携による次世代型脊柱変形矯正用インプラントの開発戦略
  須藤英毅; 金井理; 小甲晃史; 青柳健太; WANG Hao; 岩崎倫政; 千葉晶彦, 日本バイオマテリアル学会大会予稿集(Web), 40th, 165 (WEB ONLY), 2018
  Japanese
• 特発性側弯症手術に対して解剖学的脊柱配列獲得を目指して使用したロッドに対する形状類似分析手法の開発
  小甲晃史; 金井理; 安倍雄一郎; 岩崎倫政; 須藤英毅, 日本側彎症学会演題抄録集, 52nd, 276, 2018
  Japanese
• Response to Lefebvre et al
  K. Takeda; I. Kou; N. Kawakami; Y. Yasuhiko; Y. Ogura; E. Imagawa; N. Miyake; N. Matsumoto; H. Sudo; T. Kotani; Japan Early Onset Scoliosis Research Group; M. Nakamura; M. Matsumoto; Kei Watanabe; S. Ikegawa; Shohei Minami; Hiroshi Taneichi; Hideki Shigematsu; Ikuho Yonezawa; Ryo Sugawara, Clinical Genetics, 92, 5, 563, 564, 01 Nov. 2017
  Blackwell Publishing Ltd, English, Report scientific journal
• 思春期特発性側弯症におけるロッド曲線形状の類似分析手法の開発
  小甲晃史; 金井理; 安倍雄一郎; 岩崎倫政; 須藤英毅, 日本整形外科学会雑誌, 91, 8, S1795, 31 Aug. 2017
  Japanese
• 3D非対称性解析による革新的脊柱側弯症検診システムの研究開発
  須藤英毅; 小甲晃史; 林隆行; 安倍雄一郎; 岩田玲; 長枝浩; 岩崎倫政; 金井理, 日本整形外科学会雑誌, 91, 8, S1475, 31 Aug. 2017
  Japanese
• 3D非対称性解析による革新的脊柱側弯症検診システムの研究開発
  須藤英毅; 小甲晃史; 林隆行; 安倍雄一郎; 岩田玲; 長枝浩; 岩崎倫政; 金井理, 東日本整形災害外科学会雑誌, 29, 3, 368, 15 Aug. 2017
  Japanese
• 遺伝子改変マウスを用いた椎間板変性におけるcaspase 3遺伝子の果たす機能解析
  大西 貴士; 岩崎 浩司; 辻本 武尊; 岩崎 倫政; 須藤 英毅, 北海道整形災害外科学会雑誌, 58, 2, 270, 270, Mar. 2017
  北海道整形災害外科学会, Japanese
• 高純度硬化性ゲルによる無細胞移植椎間板組織自然再生誘導法
  辻本 武尊; 須藤 英毅; 岩崎 浩司; 大西 貴士; 岩崎 倫政, Journal of Spine Research, 8, 3, 298, 298, Mar. 2017
  (一社)日本脊椎脊髄病学会, Japanese
• 電子ビーム積層造形によって作製された生体用CoCrMo合金の逆変態熱処理による組織微細化
  王昊; 青柳健太; 山中謙太; 千葉晶彦; 須藤英毅, 日本金属学会講演概要(CD-ROM), 161st, 2017
• TBX6遺伝子関連の先天性側弯症における表現型と遺伝型の関連性の検討
  武田和樹; 武田和樹; 武田和樹; 黄郁代; 小倉洋二; 小倉洋二; 川上紀明; 川上紀明; 小谷俊明; 小谷俊明; 須藤英毅; 須藤英毅; 米澤郁穂; 米澤郁穂; 宇野耕吉; 宇野耕吉; 種市洋; 種市洋; 渡辺慶; 渡辺慶; 三宅紀子; 南昌平; 南昌平; 重松英樹; 重松英樹; 菅原亮; 菅原亮; 谷口優樹; 谷口優樹; 中村雅也; 松本守雄; 渡辺航太; 渡辺航太; 池川志郎, 日本側彎症学会演題抄録集, 51st, 2017
• TBX6遺伝子のnull mutationとhypomorphic risk haplotypeのcompound heterozygoteは先天性側弯症およびSpondylocostal dysostosisを引き起こす
  武田和樹; 武田和樹; 武田和樹; 黄郁代; 小倉洋二; 小倉洋二; 川上紀明; 川上紀明; 小谷俊明; 小谷俊明; 須藤英毅; 須藤英毅; 米澤郁穂; 米澤郁穂; 宇野耕吉; 宇野耕吉; 種市洋; 種市洋; 渡辺慶; 渡辺慶; 三宅紀子; 南昌平; 南昌平; 重松英樹; 重松英樹; 菅原亮; 菅原亮; 谷口優樹; 谷口優樹; 安彦行人; 中村雅也; 松本守雄; 渡邉航太; 渡邉航太; 池川志郎, 日本整形外科学会雑誌, 91, 8, 2017
• 脊柱側弯症に対する遺伝子解析の現状
  武田和樹; 武田和樹; 黄郁代; 小倉洋二; 小倉洋二; 川上紀明; 小谷俊明; 須藤英毅; 中村雅也; 松本守雄; 渡辺航太; 池川志郎, 日本小児整形外科学会雑誌, 26, 3, 2017
• 3D非対称性解析による革新的脊柱側弯症検診システムの研究開発
  須藤英毅; 小甲晃史; 林隆行; 安倍雄一郎; 岩田玲; 長枝浩; 岩崎倫政; 金井理, 日本側彎症学会演題抄録集, 51st, 160, 2017
  Japanese
• 学術奨励賞・受賞記念論文 Anomalous vertebral and posterior communicating arteries as a risk factor in cervical instrumentation surgery
  Nagahama Ken; Sudo Hideki; Abumi Kuniyoshi; Ito Manabu; Takahata Masahiko; Iwasaki Norimasa, 北海道整形災害外科学会雑誌 : 北海道整形災害外科学会機関誌, 58, 1, 15, 19, Oct. 2016
  北海道整形災害外科学会, English
• 局所麻酔薬0.5%ブピバカイン関節内頻回投与が膝関節軟骨に与える影響ラット正常膝関節及び外科的変形性関節症誘発モデルでの検討
  岩崎 浩司; 笠原 靖彦; 山田 勝久; 大西 貴士; 辻本 武尊; 岩崎 倫政; 須藤 英毅, 北海道整形災害外科学会雑誌, 58, 1, 82, 82, Oct. 2016
  北海道整形災害外科学会, Japanese
• Surgical treatment for late neurological deficits in patients with osteoporotic vertebral collapse : comparison between anterior surgery and posterior surgery
  伊東 学; 高畑 雅彦; 須藤 英毅; 金田 清志; 佐藤 栄修; 岩崎 倫政, 日本整形外科学会雑誌, 90, 9, 577, 583, Sep. 2016
  日本整形外科学会, Japanese
• 思春期特発性側彎症の進行に関与する遺伝子の発見
  小倉 洋二; 黄 郁代; 高橋 洋平; 武田 和樹; 河野 克己; 川上 紀明; 宇野 耕吉; 伊東 学; 南 昌平; 米澤 郁穂; 柳田 晴久; 種市 洋; 細金 直文; 岡田 英次朗; 辻 太一; 鈴木 哲平; 須藤 英毅; 小谷 俊明; 千葉 一裕; 戸山 芳昭; 中村 雅也; 松本 守雄; 池川 志郎; 渡邉 航太, 日本整形外科学会雑誌, 90, 8, S1502, S1502, Aug. 2016
  (公社)日本整形外科学会, Japanese
• 思春期特発性側彎症発症の分子機構の解明
  小倉 洋二; 黄 郁代; 高橋 洋平; 武田 和樹; 河野 克己; 川上 紀明; 宇野 耕吉; 伊東 学; 南 昌平; 米澤 郁穂; 柳田 晴久; 種市 洋; 細金 直文; 岡田 英次朗; 三浦 重徳; 辻 太一; 鈴木 哲平; 須藤 英毅; 小谷 俊明; 千葉 一裕; 戸山 芳昭; 開 祐司; 宿南 知佐; 中村 雅也; 松本 守雄; 池川 志郎; 渡邉 航太, 日本整形外科学会雑誌, 90, 8, S1785, S1785, Aug. 2016
  (公社)日本整形外科学会, Japanese
• 遺伝子改変マウスを用いた椎間板変性におけるcaspase 3遺伝子の果たす機能解析
  大西 貴士; 須藤 英毅; 岩崎 浩司; 辻本 武尊; 岩崎 倫政, 日本整形外科学会雑誌, 90, 8, S1685, S1685, Aug. 2016
  (公社)日本整形外科学会, Japanese
• 0.5%ブピバカインは関節軟骨に変性変化を惹起するか ラット正常膝および変形性膝関節症誘発モデルを用いた検討
  岩崎 浩司; 須藤 英毅; 笠原 靖彦; 山田 勝久; 大西 貴士; 辻本 武尊; 岩崎 倫政, 日本整形外科学会雑誌, 90, 8, S1770, S1770, Aug. 2016
  (公社)日本整形外科学会, Japanese
• 児童期に発症した腰椎椎間板ヘルニアの特徴
  小池 良直; 高畑 雅彦; 岩田 玲; 校條 祐輔; 小甲 晃史; 須藤 英毅; 岩崎 倫政, 北海道整形災害外科学会雑誌 : 北海道整形災害外科学会機関誌, 57, 2, 279, 282, Apr. 2016
  北海道整形災害外科学会, Japanese
• 椎間板造影・ブロックが正常椎間板細胞・組織に与える長期的影響
  岩崎 浩司; 山田 勝久; 大西 貴士; 辻本 武尊; 岩崎 倫政; 須藤 英毅, 北海道整形災害外科学会雑誌, 57, 2, 290, 290, Apr. 2016
  北海道整形災害外科学会, Japanese
• 再現性の高いマウス椎間板穿刺変性モデルの作成
  大西 貴士; 須藤 英毅; 岩崎 倫政, 北海道整形災害外科学会雑誌, 57, 2, 290, 291, Apr. 2016
  北海道整形災害外科学会, Japanese
• 新病理分類に基づいた再現性の高いin vivoマウス腰椎椎間板変性モデルの確立
  大西 貴士; 須藤 英毅; 岩崎 浩司; 辻本 武尊; 岩崎 倫政, Journal of Spine Research, 7, 3, 387, 387, Mar. 2016
  (一社)日本脊椎脊髄病学会, Japanese
• 遺伝子改変マウスを用いた椎間板変性におけるcaspase 3遺伝子の果たす機能解析
  大西 貴士; 須藤 英毅; 岩崎 浩司; 辻本 武尊; 岩崎 倫政, Journal of Spine Research, 7, 3, 558, 558, Mar. 2016
  (一社)日本脊椎脊髄病学会, Japanese
• 【運動器疾患のゲノム解析の最前線〜単一遺伝子病から、common disease、ビッグデータ解析まで〜】 Common diseaseのGWAS 思春期特発性側彎症
  小倉 洋二; 黄 郁代; 松本 守雄; 渡辺 航太; 池川 志郎; 河野 克己; 川上 紀明; 辻 太一; 宇野 耕吉; 鈴木 哲平; 伊東 学; 須藤 英毅; 南 昌平; 小谷 俊明; 米澤 郁穂; 柳田 晴久; 種市 洋; 千葉 一裕; 細金 直文; 岡田 英次朗; 高橋 洋平; 武田 和樹; 日本側彎症臨床研究グループ, Clinical Calcium, 26, 4, 553, 560, Mar. 2016
  (株)医薬ジャーナル社, Japanese
• TBX6のnull mutationとhypomorphic risk haplotypeのcompound heterozygoteは日本人においても先天性側弯症を引き起こす
  武田和樹; 武田和樹; 渡辺航太; 黄郁代; 小倉洋二; 三宅紀子; 川上紀明; 須藤英毅; 南昌平; 小谷俊明; 藤田順之; 池川志郎; 中村雅也; 松本守雄, Journal of Spine Research, 7, 3, 2016
• TBX6遺伝子のnull mutationとhypomorphic risk haplotypeのcompound heterozygoteは日本人においても先天性側弯症を引き起こす
  武田和樹; 渡辺航太; 小倉洋二; 川上紀明; 須藤英毅; 南昌平; 小谷俊明; 三宅紀子; 藤田順之; 池川志郎; 中村雅也; 松本守雄, 日本側彎症学会演題抄録集, 50th, 2016
• BNC2遺伝子の過剰発現が思春期特発性側弯症の発症リスクとなる
  小倉洋二; 小倉洋二; 小倉洋二; 高橋洋平; 武田和樹; 武田和樹; 河野克己; 川上紀明; 宇野耕吉; 伊東学; 南昌平; 柳田晴久; 種市洋; 米澤郁穂; 辻太一; 鈴木哲平; 須藤英毅; 小谷俊明; 岡田英次朗; 細金直文; 中村雅也; 松本守雄; 池川志郎; 渡辺航太, 日本側彎症学会演題抄録集, 50th, 2016
• 思春期特発性側弯症のゲノム解析
  小倉洋二; 小倉洋二; 小倉洋二; 高橋洋平; 武田和樹; 武田和樹; 河野克己; 川上紀明; 宇野耕吉; 伊東学; 南昌平; 柳田晴久; 種市洋; 米澤郁穂; 辻太一; 鈴木哲平; 須藤英毅; 小谷俊明; 岡田英次朗; 細金直文; 中村雅也; 松本守雄; 池川志郎; 渡辺航太, 日本小児整形外科学会雑誌, 25, 3, 2016
• 非定型抗酸菌による化膿性脊椎炎の特徴と治療経過 3例の治療経験
  校條 祐輔; 高畑 雅彦; 須藤 英毅; 長濱 賢; 黒木 圭; 小甲 晃史; 岩崎 倫政; 伊東 学; 石黒 信久, 北海道整形災害外科学会雑誌, 57, 1, 152, 152, Dec. 2015
  北海道整形災害外科学会, Japanese
• 再現性の高いマウス椎間板穿刺変性モデルの作成
  大西 貴士; 須藤 英毅; 岩崎 浩司; 辻本 武尊, 日本整形外科学会雑誌, 89, 8, S1615, S1615, Sep. 2015
  (公社)日本整形外科学会, Japanese
• 脊椎脊髄疾患に対する病態解明の現状と未来 思春期特発性側彎症に対するオーダーメード医療実現に向けた取り組み
  松本 守雄; 渡辺 航太; 高橋 洋平; 小倉 洋二; 三宅 敦; 戸山 芳昭; 川上 紀明; 辻 太一; 宇野 耕吉; 鈴木 哲平; 伊東 学; 須藤 英毅; 種市 洋; 柳田 晴久; 南 昌平; 小谷 俊明; 河野 克己; 米澤 郁穂; 池川 志郎; 長嶋 比呂志; 梅山 一大; 西脇 祐司; 道川 武紘, Journal of Spine Research, 6, 3, 206, 206, Mar. 2015
  (一社)日本脊椎脊髄病学会, Japanese
• 椎間板造影・ブロックが正常椎間板細胞・組織に与える長期的影響
  岩崎 浩司; 須藤 英毅; 山田 勝久; 大西 貴士; 辻本 武尊; 岩崎 倫政, Journal of Spine Research, 6, 3, 590, 590, Mar. 2015
  (一社)日本脊椎脊髄病学会, Japanese
• 思春期特発性側彎症の新規の疾患感受性遺伝子の同定と発症機序の解明
  小倉 洋二; 河野 克己; 川上 紀明; 宇野 耕吉; 伊東 学; 辻 太一; 鈴木 哲平; 須藤 英毅; 高橋 洋平; 渡辺 航太; 戸山 芳昭; 松本 守雄; 細金 直文; 南 昌平; 小谷 俊明; 米澤 郁穂; 柳田 晴久; 種市 洋; 千葉 一裕; 岡田 英次朗; 池川 志郎, Journal of Spine Research, 6, 3, 535, 535, Mar. 2015
  (一社)日本脊椎脊髄病学会, Japanese
• 髄核脱出を伴った小児一過性頚椎椎間板石灰化症の1例
  木田博朗; 高畑雅彦; 三田真俊; 岩田玲; 小甲晃史; 校條祐輔; 安井啓悟; 須藤英毅; 岩崎倫政, 北海道整形災害外科学会, 129th, 2015
• 強皮症に合併した頚椎calcinosisの1例
  高橋要; 新井隆太; 校條祐輔; 高畑雅彦; 須藤英毅; 長濱賢; 黒木圭; 小甲晃史; 岩崎倫政, 北海道整形災害外科学会, 128th, 107, 2015
  Japanese
• 思春期特発性側彎症のゲノムワイド相関解析 側彎の進行に関与する遺伝子の解析
  小倉 洋二; 河野 克己; 川上 紀明; 宇野 耕吉; 伊東 学; 渡辺 航太; 戸山 芳昭; 松本 守雄; 辻 太一; 鈴木 哲平; 須藤 英毅; 高橋 洋平; 南 昌平; 小谷 俊明; 柳田 晴久; 米澤 郁穂; 細金 直文; 種市 洋; 千葉 一裕; 岡田 英次朗; 池川 志郎, Journal of Spine Research, 5, 3, 251, 251, Mar. 2014
  (一社)日本脊椎脊髄病学会, Japanese
• 思春期特発性側彎症のゲノムワイド相関解析 側彎の発症に関与する遺伝子の解析
  小倉 洋二; 河野 克己; 川上 紀明; 宇野 耕吉; 伊東 学; 渡辺 航太; 戸山 芳昭; 松本 守雄; 辻 太一; 鈴木 哲平; 須藤 英毅; 高橋 洋平; 南 昌平; 小谷 俊明; 柳田 晴久; 米澤 郁穂; 細金 直文; 種市 洋; 千葉 一裕; 岡田 英次朗; 池川 志郎, Journal of Spine Research, 5, 3, 252, 252, Mar. 2014
  (一社)日本脊椎脊髄病学会, Japanese
• 思春期特発性側彎症の重症化に関連する領域を染色体17q24.3に同定
  三宅 敦; 高橋 洋平; 小倉 洋二; 黄 郁代; 河野 克己; 川上 紀明; 宇野 耕吉; 伊東 学; 南 昌平; 柳田 晴久; 種市 洋; 辻 太一; 鈴木 哲平; 須藤 英毅; 小谷 俊明; 米澤 郁穂; 渡邉 航太; 千葉 一裕; 戸山 芳昭; 松本 守雄; 池川 志郎, Journal of Spine Research, 5, 3, 421, 421, Mar. 2014
  (一社)日本脊椎脊髄病学会, Japanese
• 思春期特発性側弯症の大規模ゲノムワイド相関解析:新規の座位の同定
  小倉洋二; 小倉洋二; 松本守雄; 黄郁代; 高橋洋平; 河野克己; 川上紀明; 宇野耕吉; 伊東学; 南昌平; 米澤郁穂; 辻太一; 鈴木哲平; 須藤英毅; 渡邉航太; 戸山芳昭; 池川志郎, 日本整形外科学会雑誌, 88, 8, 2014
• 思春期特発性側弯症の原因遺伝子-遺伝子診断の現況
  高橋洋平; 高橋洋平; 高橋洋平; 小倉洋二; 小倉洋二; 黄郁代; 高橋弘; 河野克己; 川上紀明; 辻太一; 宇野耕吉; 鈴木哲平; 伊東学; 須藤英毅; 南昌平; 小谷俊明; 柳田晴久; 種市洋; 米澤郁穂; 渡辺航太; 戸山芳昭; 松本守雄; 池川志郎, 日本側彎症学会演題抄録集, 48th, 2014
• 思春期特発性側弯症のゲノムワイド相関解析:新規の疾患受性遺伝子の発現
  小倉洋二; 小倉洋二; 小倉洋二; 河野克己; 川上紀明; 宇野耕吉; 伊藤学; 南昌平; 米澤郁穂; 柳田晴久; 種市洋; 岡田英次朗; 高橋洋平; 辻太一; 鈴木哲平; 須藤英毅; 小谷俊明; 渡辺航太; 細金直文; 千葉一裕; 戸山芳昭; 松本守雄; 池川志郎, 日本側彎症学会演題抄録集, 48th, 2014
• 側弯症矯正手術における内固定金属の力学分担:クロスリンクによる応力分散効果
  安倍雄一郎; 伊東学; 鐙邦芳; 藤崎和弘; REMEL Salmingo; 但野茂; 久田雄一郎; 高畑雅彦; 須藤英毅; 長濱賢; 中原誠之, J Spine Res, 4, 3, 750, 25 Mar. 2013
  Japanese
• 思春期特発性側彎症の進行に関する遺伝子マーカーの検討 日本人での大規模再現解析
  小倉 洋二; 高橋 洋平; 河野 克己; 川上 紀明; 宇野 耕吉; 伊東 学; 南 昌平; 柳田 晴久; 米澤 郁穂; 種市 洋; 辻 太一; 鈴木 哲平; 須藤 英毅; 小谷 俊明; 渡辺 航太; 戸山 芳昭; 千葉 一裕; 松本 守雄; 池川 志郎, Journal of Spine Research, 4, 3, 713, 713, Mar. 2013
  (一社)日本脊椎脊髄病学会, Japanese
• 思春期特発性側弯症の遺伝子解析
  高橋洋平; 高橋洋平; 河野克己; 川上紀明; 宇野耕吉; 伊東学; 南昌平; 柳田晴久; 種市洋; 米澤郁穂; 渡辺航太; 辻太一; 鈴木哲平; 須藤英毅; 戸山芳昭; 池川志郎; 松本守雄, Journal of Spine Research, 4, 3, 2013
• Caspase 3 as a Therapeutic Target for Regulation of Intervertebral Disc Degeneration in Rabbits
  須藤 英毅; 三浪 明男, 北海道醫學雜誌 = Acta medica Hokkaidonensia, 87, 6, 258, 258, 01 Nov. 2012
  Japanese
• Reconstruction of the Subaxial Cervical Spine Using Pedicle Screw Instrumentation
  鐙 邦芳; 伊東 学; 須藤 英毅, 北海道醫學雜誌 = Acta medica Hokkaidonensia, 87, 6, 260, 260, 01 Nov. 2012
  Japanese
• 2012(基礎部門優秀論文賞) Caspase 3 as a Therapeutic Target for Regulation of Intervertebral Disc Degeneration in Rabbits (平成24年度助成研究論文)
  須藤 英毅; 三浪 明男, 未病と抗老化 : 財団法人博慈会老人病研究所紀要, 21, 101, 111, Jun. 2012
  博慈会老人病研究所, English
• 【後縦靱帯骨化症(OPLL)の治療】胸椎OPLLの治療 胸椎OPLLに対する後方進入全周性脊髄除圧
  鐙 邦芳; 高畑 雅彦; 伊東 学; 須藤 英毅; 長濱 賢, 関節外科, 31, 5, 578, 585, May 2012
  (株)メジカルビュー社, Japanese
• 内固定金属形状追跡による側弯症矯正手術の力学解析
  安倍雄一郎; 伊東学; 鐙邦芳; 藤崎和弘; REMEL Salmingo; 但野茂; 小谷善久; 須藤英毅; 長濱賢; 岩田玲; 佐藤栄修; 三浪明男, J Spine Res, 3, 3, 448, 25 Mar. 2012
  Japanese
• 思春期特発性側彎症の全ゲノム相関解析
  高橋 洋平; 河野 克己; 川上 紀明; 宇野 耕吉; 伊東 学; 南 昌平; 柳田 晴久; 種市 洋; 渡辺 航太; 辻 太一; 鈴木 哲平; 須藤 英毅; 小谷 俊明; 千葉 一裕; 戸山 芳昭; 池川 志郎; 松本 守雄, Journal of Spine Research, 3, 3, 447, 447, Mar. 2012
  (一社)日本脊椎脊髄病学会, Japanese
• 当院の脊柱側弯症手術におけるMEPモニタリングの成績
  村上望; 山本雅史; 奥原浩之; 鐙邦芳; 伊東学; 小谷善久; 須藤英毅; 高畑雅彦; 長濱賢; 岩田玲; 三浪明男, 北海道整形災害外科学会, 122nd, 2012
• 思春期特発性側弯症の進行予測キットScoliscoreの有用性の検証:日本人での大規模再現解析
  小倉洋二; 高橋洋平; 河野克己; 川上紀明; 宇野耕吉; 伊東学; 南昌平; 渡辺航太; 戸山芳昭; 辻太一; 鈴木哲平; 須藤英毅; 松本守雄; 小谷俊明; 柳田晴久; 種市洋; 米澤郁穂; 池川志郎, 日本側彎症学会演題抄録集, 46th, 2012
• 中国人で同定された思春期特発性側弯症の進行に関与するSNP:日本人での大規模再現解析
  小倉洋二; 高橋洋平; 河野克己; 川上紀明; 宇野耕吉; 伊東学; 南昌平; 渡辺航太; 戸山芳昭; 松本守雄; 辻太一; 鈴木哲平; 須藤英毅; 小谷俊明; 柳田晴久; 種市洋; 米澤郁穂; 池川志郎, 日本側彎症学会演題抄録集, 46th, 2012
• 特発性側弯症後方矯正手術時における脊椎インプラントの応力評価
  伊東学; 安倍雄一郎; 鐙邦芳; 藤崎和弘; SALMINGO Remel; 但野茂; 小谷善久; 須藤英毅; 長濱賢; 岩田玲; 三浪明男, J Spine Res, 2, 3, 609, 25 Mar. 2011
  Japanese
• CT osteoabsorptmetry法を用いた小児特発性側弯症における椎体終板骨濃度分布の解析
  安倍雄一郎; 伊東学; 鐙邦芳; 藤崎和弘; 但野茂; 岩崎倫政; 小谷善久; 須藤英毅; 長濱賢; 岩田玲; 三浪明男, J Spine Res, 2, 3, 615, 25 Mar. 2011
  Japanese
• Comparative Study between Hybrid System and Direct Vertebral Rotation Procedure with Pedicle Screws in Treatment of Adolescent Idiopathic Scoliosis
  OSHIMA Shigeki; ITO Manabu; TAKAHATA Masahiko; ABUMI Kuniyoshi; KOTANI Yoshihisa; SUDO Hideki; HOJO Yoshihiro; ABE Yuichiro; MINAMI Akio, Journal of spine research, 1, 11, 2074, 2078, 25 Nov. 2010
  日本脊椎脊髄病学会, Japanese
• Posterolateral spinal endoscopic debridement and irrigation for postoperative spinal infections
  伊東 学; 放生 憲博; 須藤 英毅, Orthopaedic surgery and traumatology, 53, 5, 647, 653, Apr. 2010
  金原出版, Japanese
• 内固定金属形状追跡による側弯症矯正手術の力学解析
  安倍雄一郎; 伊東学; 鐙邦芳; 藤崎和弘; SALMINGO Remel; 但野茂; 小谷善久; 須藤英毅; 長濱賢; 岩田玲; 三浪明男, 日本側わん症学会演題抄録集, 44th, 69, 2010
  Japanese
• 【脊柱後彎症の病態と手術治療】脊椎高位による後彎症治療の特異性 頭蓋頸椎移行部の後彎変形 関節リウマチによる頭蓋頸椎移行部後彎の病態と矯正固定手術
  高畑 雅彦; 鐙 邦芳; 伊東 学; 小谷 善久; 須藤 英毅; 放生 憲博, 脊椎脊髄ジャーナル, 22, 5, 639, 645, May 2009
  (株)三輪書店, Japanese
• Spinal Reconstruction Surgery using Ultra-high Molecular Weight Polyethylene Cable
  TAKAHATA Masahiko, 日本脊椎脊髄病学会雑誌 = The journal of the Japan Spine Research Society, 19, 4, 724, 729, 25 Dec. 2008
  Japanese
• Posterolateral Endoscopic Debridement and Irrigation for Pyogenic Spondylitis
  ITO Manabu, 日本脊椎脊髄病学会雑誌 = The journal of the Japan Spine Research Society, 19, 4, 741, 747, 25 Dec. 2008
  Japanese
• Atlantoaxial instability with ankylosing spondylitis: a case report
  林 晴久; 須藤 英毅; 鐙 邦芳, Orthopedic surgery, 59, 9, 1095, 1097, Aug. 2008
  南江堂, Japanese
• Revision Surgery Using Pedicle Screw Fixation Systems for Previously Operated Cervical Spine
  ABUMI Kuniyoshi; ITO Manabu; KOTANI Yoshihisa; TAKAHATA Masahiko; SUDO Hideki; HOJO Yoshihiro; MINAMI Akio, 日本脊椎脊髄病学会雑誌 = The journal of the Japan Spine Research Society, 19, 2, 359, 359, 20 Mar. 2008
  English
• Cervical Spinal Cord Tumor Requring Spinal Reconstructive Surgery
  ABUMI K, 日本脊椎脊髄病学会雑誌 = The journal of the Japan Spine Research Society, 19, 1, 41, 41, 20 Mar. 2008
  Japanese
• Minimum invasive posterior reconstruction surgery for lumbar degenerative disorders using subcutaneous pedicle screw placement with emphasis on perioperative invasiveness and QOL
  KOTANI Y, 日本脊椎脊髄病学会雑誌 = The journal of the Japan Spine Research Society, 19, 1, 68, 68, 20 Mar. 2008
  Japanese
• Review of surgical treatment for osteoporotic vertebral fracture and its future direction
  ITO M, 日本脊椎脊髄病学会雑誌 = The journal of the Japan Spine Research Society, 19, 2, 250, 250, 20 Mar. 2008
  Japanese
• C5 Palsy after Correction of Cervical Kyphosis using Pedicle Screw System
  HOJO Y, 日本脊椎脊髄病学会雑誌 = The journal of the Japan Spine Research Society, 19, 2, 488, 488, 20 Mar. 2008
  Japanese
• Efficacy of Linezolid in the treatment of refractory MRSA spondylitis
  T. Masahiko, 日本脊椎脊髄病学会雑誌 = The journal of the Japan Spine Research Society, 19, 2, 491, 491, 20 Mar. 2008
  Japanese
• School Screening for Scoliosis in Sapporo-city from 1989 through 2005
  SUKEGAWA Atsushi, 脊柱変形 : 日本側彎症研究会会誌 = Spinal deformity : the journal of Japanese Scoliosis Society, 22, 1, 31, 35, 20 Dec. 2007
  Japanese
• プロテウス症候群様身体異常を合併した脊髄症・神経根症2例の治療経験
  新井隆太; 伊東 学; 小谷 善久; 高畑 雅彦; 須藤 英毅; 大嶋 茂樹; 三浪 明男; 鐙 邦芳, 北海道整形災害外科学会雑誌, 48, 2, 116, 117, Mar. 2007
  Japanese, Summary national conference
• Surgical Treatment of Cervical Spondylotic Myelopathy with Kyphosis : Posteriror Decompression and Correction of Kyphosis
  ABUMI Kuniyoshi; ITO Manabu; KOTANI Yoshihisa; SUDO Hideki; TAKAHATA Masahiko; OOSHIMA Shigeki; MINAMI Akio, 日本脊椎脊髄病学会雑誌 = The journal of the Japan Spine Research Society, 17, 1, 7, 8, 20 Mar. 2006
  Japanese
• Procedural Changes of Spinal Reconstruction Surgery for Osteoporotic Vertebral Collapse : Based on the Long-term Clinical Results of Spinal Reconstruction Surgery
  ITO Manabu; ABUMI Kiniyoshi; KOTANI Yoshihisa; TAKAHATA Masahiko; SUDO Hideki; OHSHIMA Shigeki; KANEDA Kiyoshi; FUJIYA Masanori; MINAMI Akio, 日本脊椎脊髄病学会雑誌 = The journal of the Japan Spine Research Society, 17, 1, 155, 156, 20 Mar. 2006
  Japanese
• Biomechanical Effects of PLF Alone and PLIF/PLF on the Adjacent Segment
  須藤 英毅; 鐙 邦芳; 織田 格, 臨床整形外科, 38, 4, 487, 491, Apr. 2003
  医学書院, Japanese
• What types of instability require intervertebral support in the posterior lumbar reconstruction?
  ODA I, 日本脊椎脊髄病学会雑誌 = The journal of the Japan Spine Research Society, 13, 1, 2, 2, 26 Apr. 2002
  Japanese
• Biomechanical effects of spinal reconstruction on its adjacent segment : an In-Vitro comparison of PLF alone and PLF combined with PLIF
  SUDO H, 日本脊椎脊髄病学会雑誌 = The journal of the Japan Spine Research Society, 13, 1, 13, 13, 26 Apr. 2002
  Japanese
• Risk factor analysis for osteoporotic vertebral collapse
  TANEICHI H., 日本脊椎脊髄病学会雑誌 = The journal of the Japan Spine Research Society, 12, 1, 215, 215, 27 Apr. 2001
  Japanese
• Spinal injuries in snowboarders
  SUDOH H, 日本脊椎外科学会雑誌 = The journal of the Japan Spine Research Society, 11, 1, 356, 356, 28 Apr. 2000
  Japanese

• 整形外科SURGICAL TECHNIQUE 特発性側弯症に対する基本手術手技 矯正・固定のポイント
  須藤英毅, 4D解剖学的矯正
  メディカ出版, Jan. 2025, [Contributor]
• 小児脊柱変形治療の最前線
  須藤英毅 (担当:手術治療,手術手技,Dual-rod Rotation, 179-183), 手術治療，手術手技，Dual-rod Rotation,179-183
  南江堂，東京, 2021, [Single work]
• 小児脊柱変形治療の最前線
  須藤英毅(担当:診療の考え方,トピック:3D対称性解析による脊柱側弯症検診システム,57-58), 診療の考え方，トピック：3D対称性解析による脊柱側弯症検診システム,57-58
  南江堂，東京, 2021, [Single work]
• 整形外科手術Knack and Pitfallsシリーズ，脊椎脊髄病における脊椎インストゥメンテーション手術
  須藤英毅(担当:脊柱変形に対する脊椎インストゥメンテーション手術,骨切り術を使用した矯正固定術,Ponte骨切り術(Grade 2),114-121), 脊柱変形に対する脊椎インストゥメンテーション手術，骨切り術を使用した矯正固定術，Ponte骨切り術（Grade 2）
  文光堂，東京, 2021, [Single work]
• Cervical Spine Surgery: Standard and Advanced Techniques. Chapter 37; SURGICAL TECHNIQUES - UPPER CERVICAL - C2 pedicle screw fixation technique
  Hideki Sudo; Kuniyoshi Abumi
  Springer, 2018
• Neurological and vascular complications after surgery for cervical myelopathy
  Hideki Sudo, Chapter 20
  Jaypee Medical Publishers, 2016, [Contributor]
• Complications of Spinal Hydatidosis
  Hideki Sudo, Chapter 18,213-222
  Springer-Verlag Berlin Heidelberg, 2014, [Contributor]
• 講義録運動器学
  須藤英毅; 三浪明男, 前腕遠位部骨折，165-168
  medical view，東京, 2006, [Joint work]
• 講義録運動器学
  須藤英毅; 三浪明男, 手根骨骨折，169-172
  medical view，東京, 2006, [Joint work]

• 基本医学総論, 2024年, 修士課程, 医学院
• 基本医学研究, 2024年, 修士課程, 医学院
• 医学総論, 2024年, 博士後期課程, 医学研究科
• 基本医学総論, 2024年, 修士課程, 医学院
• 医学総論, 2024年, 博士後期課程, 医学院
• 医学総論, 2024年, 博士後期課程, 医学院
• 基盤医学研究, 2024年, 博士後期課程, 医学院
• 臨床医学研究, 2024年, 博士後期課程, 医学院

• THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE
• 日本側弯症学会
• THE JAPANESE SOCIETY FOR SPINE SURGERY AND RELATED RESEARCH
• THE JAPANESE ORTHOPAEDIC ASSOCIATION

• 超高純度幹細胞・バイオマテリアルによる椎間板再生メカニズムの包括的解明
  科学研究費補助金基盤研究（A）
  Apr. 2024 - Mar. 2028
  須藤英毅
  文部科学省 科学研究費補助金, Principal investigator
• 椎間板保水能評価のための非侵襲的イメージングシステム開発
  科学研究費助成事業
  01 Apr. 2024 - 31 Mar. 2027
  Tha KhinKhin; 須藤 英毅
  日本学術振興会, 基盤研究(C), 北海道大学, 24K15693
• 高生体適合性三次元積層造形椎体間ケージの開発
  Jul. 2024 - Mar. 2025
  須藤英毅
  ノーステック財団 札幌バイオシーズ事業化支援事業
• アナトミカルロッドとユニバーサルヘッドスクリューによる経皮的脊椎固定システムの開発・事業化
  北海道銀行中小企業技術活性化助成金
  Mar. 2024 - Mar. 2025
  須藤英毅
• アナトミカルロッドとユニバーサルヘッドスクリューによる経皮的脊椎固定システムの開発・事業化
  北洋銀行スタートアップ研究開発基金
  Mar. 2024 - Mar. 2025
  須藤英毅
• アナトミカルロッドとユニバーサルヘッドスクリューによる経皮的脊椎固定システムの開発・事業化
  医工連携イノベーション推進事業 開発･事業化事業（医療機器ベンチャー育成）
  Oct. 2023 - Mar. 2025
  須藤英毅
  日本医療研究開発機構(AMED)
• 腰部脊柱管狭窄症に対するREC/dMD-001の安全性と有効性に係る探索的医師主導治験
  Apr. 2022 - Mar. 2025
  須藤英毅
  日本医療研究開発機構(AMED) 再生医療実用化研究事業
• 自家骨髄濃縮液による低侵襲椎間板組織再生誘導法の開発と組織再生メカニズムの解明
  科学研究費補助金基盤研究（B）
  Apr. 2021 - Mar. 2024
  須藤英毅
  Principal investigator
• 高純度同種間葉系幹細胞(REC)と硬化性ゲルを用いた腰部脊柱管狭窄症に対する細胞治療
  再生医療実現拠点ネットワークプログラム （技術開発個別課題）
  Jun. 2020 - Mar. 2022
  須藤英毅
  日本医療研究開発機構(AMED)
• 無形医療技術のデータ化及び分析に基づいた脊椎外科手術用高生体適合性医療機器の開発
  2022
  須藤英毅
  鈴木謙三記念医科学応用研究財団
• 混合性腰部脊柱管狭窄症に対する高純度同種間葉系幹細胞（REC）を用いた新規治療法の開発
  革新的医療技術創出拠点プロジェクト橋渡し研究戦略的推進プログラム preB
  Apr. 2020 - Mar. 2021
  須藤英毅
  日本医療研究開発機構(AMED)
• 椎間板再生治療における組織修復材の開発
  革新的医療技術創出拠点プロジェクト 橋渡し研究戦略的推進プログラム シーズC
  Apr. 2018 - Mar. 2021
  須藤英毅
  日本医療研究開発機構(AMED), Principal investigator, Competitive research funding
• 椎間板変性に起因した痛みと関連する血清中糖鎖の網羅的解析
  科学研究費補助金挑戦的研究(萌芽)
  Jul. 2018 - Mar. 2020
  須藤英毅
  文部科学省, Principal investigator, Competitive research funding
• 高純度硬化性ゲルによる椎間板組織自然再生誘導法の開発と組織再生メカニズムの解明
  科学研究費補助金基盤研究（B）
  Apr. 2016 - Mar. 2019
  須藤英毅
  Principal investigator, Competitive research funding
• 高適合・短時間施術を実現する、3Dモデリング及び3D積層造形を応用したカスタムメイド脊柱変形矯正用インプラントの開発・事業化
  医工連携事業化推進事業
  2016 - 2019
  須藤英毅
  日本医療研究開発機構(AMED), Principal investigator, Competitive research funding
• 知財活用支援事業 大学等知財基盤強化支援（権利化支援）
  2018
  須藤英毅
  科学技術振興機構(JST), Principal investigator, Competitive research funding
• 知財活用支援事業 大学等知財基盤強化支援
  Feb. 2017
  須藤英毅
  科学技術振興機構(JST), Principal investigator, Competitive research funding
• Factors affecting implant rod deformation in adolescent idiopathic scoliosis surgery
  2016 - 2017
  須藤英毅
  AOSpine Research Grant, Principal investigator, Competitive research funding
• 椎間板再生治療における組織修復材の開発
  革新的医療技術創出拠点プロジェクト 橋渡し研究加速ネットワークプログラムシーズB
  2016 - 2017
  須藤英毅
  日本医療研究開発機構(AMED), Principal investigator, Competitive research funding
• Maintenance of homeostasis for intervertebral disc by cell cycle-related genes
  Grants-in-Aid for Scientific Research
  2014 - 2015
  Sudo Hideki
  Although human intervertebral disc (IVD) degeneration can cause several spinal diseases, its pathogenesis remains unclear. This study aimed to create a highly reproducible in vivo mouse IVD degeneration model. One hundred six mice were operated and L4/5 IVD was stabbed with a 35 or 33-gauge needle. Micro-computed tomography scanning was performed, and the stabbed region was confirmed. Evaluation was by magnetic resonance imaging (MRI) and histology using our classification scoring system. In IVDs stabbed with either needle, significant degeneration occurred relative to the control. Our histological classification scores correlated well with MRI findings and could detect degenerative progression, irrespective of the stabbed region.In this study, a highly reproducible in vivo mouse IVD degeneration model was created with precise information about surgical techniques.
  Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, Principal investigator, Competitive research funding, 26670651
• Caspase 3 Silencing Inhibits Biomechanical Overload-induced Intervertebral Disc Degeneration
  Grants-in-Aid for Scientific Research
  2013 - 2013
  SUDO HIDEKI
  Intervertebral disc (IVD) degeneration causes debilitating low back pain in large sections of the worldwide population. No efficient treatment currently exists because the unclear pathogenesis. One characteristic event early in the degeneration is the apoptosis of nucleus pulposus (NP) cells embedded in IVD. Excessive biomechanical loading may be also a major etiology of IVD degeneration. The present study used in vitro and in vivo models of compressive loading to elucidate the underlying mechanism of IVD degeneration. In addition, we investigated whether the inhibition of apoptosis is a potential clinical therapeutic strategy for the treatment of IVD degeneration induced by biomechanical stress.The present study suggests that caspase 3 siRNA attenuates overload-induced IVD degeneration by inhibiting NP cell apoptosis and the expression of matrix-degrading enzymes.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Principal investigator, Competitive research funding, 23592150
• Caspase 3 Silencing Inhibits Biomechanical Overload-induced Intervertebral Disc Degeneration
  Grants-in-Aid for Scientific Research
  2011 - 2013
  SUDO HIDEKI
  Intervertebral disc (IVD) degeneration causes debilitating low back pain in large sections of the worldwide population. No efficient treatment currently exists because the unclear pathogenesis. One characteristic event early in the degeneration is the apoptosis of nucleus pulposus (NP) cells embedded in IVD. Excessive biomechanical loading may be also a major etiology of IVD degeneration. The present study used in vitro and in vivo models of compressive loading to elucidate the underlying mechanism of IVD degeneration. In addition, we investigated whether the inhibition of apoptosis is a potential clinical therapeutic strategy for the treatment of IVD degeneration induced by biomechanical stress.The present study suggests that caspase 3 siRNA attenuates overload-induced IVD degeneration by inhibiting NP cell apoptosis and the expression of matrix-degrading enzymes.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Principal investigator, Competitive research funding, 23592150
• Nakatomi Foundation
  2012
  Hideki Sudo
  Principal investigator, Competitive research funding
• Suzuken Memorial Foundation
  2011
  Hideki Sudo
  Principal investigator, Competitive research funding
• Takeda Science Foundation
  2011
  Hideki Sudo
  Principal investigator, Competitive research funding
• Regulation of cell cycle in nucleus pulposus cells for the treatment of intervertebral disc degeneration
  Grants-in-Aid for Scientific Research(基盤研究(C))
  2008 - 2010
  Hideki SUDO
  Although the etiology of intervertebral disc degeneration is poorly understood, one possible approach for the treatment includes regulation of cell cycle in nucleus pulposus cells. We investigated the cell cycle effects of nucleus pulposus cells in intervertebral disc degeneration. Our findings indicate that regulation of cell cycle in intervertebral disc cells is effective in preventing intervertebral disc degeneration.
  Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(C), 北海道大学, Principal investigator, Competitive research funding, 20591733
• Japan Orthopaedics and Traumatology Foundation (JOTF)
  2008
  Hideki Sudo
  Principal investigator, Competitive research funding
• アポトーシス関連遺伝子を用いた椎間板組織の細胞周期・老化変性の制御機構の解明
  科学研究費補助金(若手研究(B))
  2005 - 2007
  須藤 英毅
  これまで,椎間板の変性変化には椎間板細胞のアポトーシスが関与している可能性があることが示唆されていたが,その詳細なメカニズムは依然不明であった.本研究ではアポトーシスにおいて直接的な役割を果たす遺伝子群と,椎間板細胞を用いた遺伝子導入実験を行い,椎間板細胞のアポトーシス誘導機構の解析とこれらが椎間板変性に及ぼすメカニズムについての検討を行った.ラットアポトーシス抑制遺伝子:Bcl-2に対する最適化遺伝子合成を行い発現プラスミドを構築した.これをラット椎間板より採取拡大培養した椎間板細胞に遺伝子導入した.遺伝子導入48時間後に血清飢餓を開始しアポトーシスを誘導した.Bcl-2を過剰発現した系では,コントロール群に比べTunne1法にて断片化DNAが減少し,FACSにおいてもアポトーシス陽性細胞の減少が確認された.同時にRT-PCRにてCaspase3の発現量が低下していた.つまり,Bcl-2の過剰発現はCaspase3によるアポトーシスの実行を抑制することが明らかになった.椎間板細胞アポトーシスを遺伝子レベルで抑制することで椎間板変性に対する初期治療に応用できると思われる.
  文部科学省, 若手研究(B), 北海道大学, Principal investigator, Competitive research funding, 17790991
• Nakatomi Foundation
  2005
  Hideki Sudo
  Principal investigator, Competitive research funding
• Uehara Memorial Foundation
  2005
  Hideki Sudo
  Principal investigator, Competitive research funding

• ROD GROUP, ARCUATE ROD, S-SHAPED ROD, SPINE STABILIZATION SYSTEM, AND ROD MANUFACUTURING METHOD
  Patent right, Hideki Sudo; Satoshi Kanai; Terufumi Kokabu; Yuichiro Abe; Tsuyoshi Segawa
  特許US 11,622,792 B2
  Hokkaido University, Robert Reid INC.
• 椎間板再生用組成物
  Patent right, 須藤英毅; 筌場大介; 松崎有未; 陶山隆史
  特願2021-13667, 29 Jan. 2021
• 椎間板疼痛抑制用組成物
  Patent right, 須藤英毅; 浦勝郎; 山田勝久
  PCT/JP2020/042482, 13 Nov. 2020
• 側弯症診断支援装置、側弯症診断支援システム、機械学習装置、側弯症診断支援方法、機械学習方法及びプログラム
  Patent right, 須藤英毅; 金井理; 小甲晃史; 安倍雄一郎; 瀬川剛
  特願2020-145623, 31 Aug. 2020
• Continuously Variable Simulation (CVS) spinal system
  Design right, 須藤英毅; 株式会社ロバートリード商会
  T19XX044
• 一組の弓状ロッドおよび一組のS字状ロッド
  Patent right, 須藤英毅; 金井理; 小甲晃史; 安倍雄一郎; 瀬川剛
  特願2019-052987, 20 Mar. 2019
• SCOLIOMAP
  Design right, Hideki Sudo
  基礎出願 商願2019-004102
  1469015(国際登録番号) 日本、米国、欧州連合、中国
  Hideki Sudo, Robert Reid Inc.
• 椎間板治療用組成物
  Patent right, 須藤英毅; 辻本武尊; 岩崎倫政; 清水賢; 伊佐次三津子
  特願2019-027933, 20 Feb. 2019
• 脊柱変形矯正固定術支援装置、脊柱変形矯正固定術支援方法、プログラム、及び脊柱変形矯正固定術に使用するロッド製造方法
  Patent right, 須藤英毅; 山本強; 安倍雄一郎; 岩崎倫政
  特願2014-226466, 06 Nov. 2014
  特許第6633353号
• ロッド群、弓状ロッド群、脊柱安定化システム、およびロッドの製造方法
  Patent right, 須藤英毅; 金井理; 小甲晃史; 安倍雄一郎; 瀬川剛
  特許第6502587号
• 椎間板治療用組成物
  Patent right, 須藤英毅; 辻本武尊; 岩崎倫政; 清水賢; 伊佐次三津子
  TH1801005621
• 椎間板治療用組成物
  Patent right, 須藤英毅; 辻本武尊; 岩崎倫政; 清水賢; 伊佐次三津子
  BR-2 1220210247099
• 椎間板治療用組成物
  Patent right, 須藤英毅; 辻本武尊; 岩崎倫政; 清水賢; 伊佐次三津子
  BR-1 1220210152535
• 椎間板治療用組成物
  Patent right, 須藤英毅; 辻本武尊; 岩崎倫政; 清水賢; 伊佐次三津子
  BR1120180690600
• 椎間板治療用組成物
  Patent right, 須藤英毅; 辻本武尊; 岩崎倫政; 清水賢; 伊佐次三津子
  CA3018152
• 椎間板治療用組成物
  Patent right, 須藤英毅; 辻本武尊; 岩崎倫政; 清水賢; 伊佐次三津子
  CN201780018143.7
• 椎間板治療用組成物
  Patent right, 須藤英毅, 辻本武尊, 岩崎倫政, 清水賢, 伊佐次三津子
  EP17769650.7
• 椎間板治療用組成物
  Patent right, 須藤英毅; 辻本武尊; 岩崎倫政; 清水賢; 伊佐次三津子
  US16/086081
• 椎間板治療用組成物
  Patent right, 須藤英毅; 辻本武尊; 岩崎倫政; 清水賢; 伊佐次三津子
  特願2021-107910
• 椎間板治療用組成物
  Patent right, 須藤英毅; 辻本武尊; 岩崎倫政; 清水賢; 伊佐次三津子
  特許第6907254号
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