PhD, Hokkaido University, Mar. 2025

May 2025, 第79回NPO法人日本口腔科学会学術集会, Rising Scientist賞
細胞外基質ラミニンは褐色細胞腫由来PC-12細胞の脱分化を抑制し、本来の副腎髄質細胞機能を回復する

大学病院小児・障がい者歯科における5年間の有病児および障害児(者)の初診時実態
西浦 まい; 青山 佳仁; 須貝 壮; 野村 奈央子; 中村 光一; 八若 保孝
小児歯科学雑誌, 64, 2, 33, 40, (公社)日本小児歯科学会, Jun. 2026
Japanese

Bone Metabolic Changes and Osteoporosis During Pregnancy and Lactation: A View from Dental Medicine.
Mai Nishiura; Haruhisa Watanabe; Atsuko Nakanishi-Kimura; Marie Hoshi-Numahata; Shinnosuke Nishimoto; Fumi Ueno; Riyu Koguchi; Ryutaro Takemoto; Yusuke Kurakane; Lang Bao; Tadahiro Iimura
International journal of molecular sciences, 26, 21, 28 Oct. 2025, [International Magazine]
English, Scientific journal, Pregnancy- and lactation-associated osteoporosis (PLO) is receiving increasing attention. During pregnancy and lactation, bone metabolism is dramatically changed to supply minerals to the fetus and infant, which is a major cause of PLO. Weaning of lactation is clinically a primary choice to treat lactation-induced osteoporosis since breastfeeding is a key regulator of the pathophysiology during lactation. However, breastfeeding is beneficial to the physical and mental development of infants. We also discuss the beneficial effects of breastfeeding on the oral and maxillofacial development of infants. Pharmacological treatment of PLO is also discussed. This review also discusses how dynamic regulatory changes in bone metabolism during pregnancy and lactation affect homeostasis of the temporomandibular joint (TMJ) and alveolar bone in mothers, from the perspectives of TMJ diseases and orthodontic treatment.

細胞外基質ラミニンは褐色細胞腫由来PC-12細胞の脱分化を抑制し,本来の副腎髄質細胞機能を回復する
西浦 まい; 渡辺 陽久; 飯村 忠浩
日本口腔科学会雑誌, 74, 2, 118, 118, (NPO)日本口腔科学会, Jul. 2025
Japanese

A Long-Term (41 Years) Radiographic Follow-Up Study of the Onset and Development of a Jawbone Lesion in a Patient With Gnathodiaphyseal Dysplasia.
Riyu Koguchi; Haruhisa Watanabe; Mai Nishiura; Tadahiro Iimura; Yutaka Maruoka
Cureus, 17, 7, e88763, Jul. 2025, [International Magazine]
English, Scientific journal, Gnathodiaphyseal dysplasia (GDD) is an autosomal dominant syndrome characterized by bone fragility, sclerosis of tubular bones, and cemento-osseous lesions of the jawbones. This report describes the long-term radiographic follow-up study of jaw lesions in a GDD-affected woman of >40 years of age from the age of one onward. At three years of age, widening of the diaphyseal cortices in the femur and radius was observed, although no symptoms were observed in the jawbones. At nine years of age, we observed a slightly sclerotic appearance in the alveolar and jawbones adjacent to the permanent tooth roots. After completion of the secondary dentition at 14 years of age, increased density of the sclerotic mass was clearly observed. Although the etiology and pathogenesis are uncertain, this study observed that the onset of the jawbone lesion had already appeared after the mixed dentition stage, and the sclerotic mass developed with age.

Genome-wide analyses of susceptibility genes responsible for mandibular prognathism in the Japanese population.
Marie Hoshi-Numahata; Atsuko Nakanishi-Kimura; Haruhisa Watanabe; Mai Nishiura; Shinnosuke Nishimoto; Fumi Ueno; Riyu Koguchi; Akira Oka; Yoshiaki Sato; Takashi S Kajii; Tadahiro Iimura
Journal of oral biosciences, 67, 2, 100670, 100670, Jun. 2025, [International Magazine]
English, Scientific journal, BACKGROUND: Mandibular prognathism (MP) is a type of malocclusion characterized by an imbalance in the anteroposterior position of the upper and lower jaws. The prevalence of MP in Japan is relatively high, suggesting a unique genetic background in the population. HIGHLIGHT: Genome-wide analyses identified susceptibility genes responsible for mandibular prognathism in the Japanese population. CONCLUSION: Identification of the genes associated with malocclusion will pave the way for personalized and precise medicine and contribute to craniofacial biology.

Cholesterol metabolism and neuroinflammatory changes in a non-human primate spinal nerve ligation model.
Hiroshi Yamane; Suguru Koyama; Takayuki Komatsu; Tomoya Tanaka; Riyu Koguchi; Haruhisa Watanabe; Mai Nishiura; Satoru Yoshikawa; Tadahiro Iimura
Scientific reports, 15, 1, 11462, 11462, 03 Apr. 2025, [International Magazine]
English, Scientific journal, Neuropathic pain remains one of the major neurological conditions with high unmet medical needs. Poor translation from preclinical studies using rodent models to clinical trials is one of the major obstacles to the development of new pharmacological medications to treat neuropathic pain. The aims of this study were to establish a behavioral test to evaluate spontaneous pain in a spinal nerve ligation (SNL) model using cynomolgus monkey as a non-human primate (NHP) model. After right unilateral L7 SNL surgery in cynomolgus monkeys, the percentage of weight-bearing on ipsilateral hindlimb significantly decreased, which was well-associated with an analytical score of electroencephalography (EEG). Transcriptomic analysis of RNA-seq results from the dorsal part of the spinal cord identified pathways matching those in equivalent rodent models, along with NHP-specific pathways, suggesting that neuroinflammation and cholesterol transportation/metabolism were the main pathways altered in this NHP model. Additionally, several upregulated genes observed here were previously reported uniquely in clinical studies, but not in rodent models. This study provides a potentially useful model that can aid our understanding of pathophysiological mechanism of neuropathic pain and the development of pain relief therapies by inducing a robust behavioral phenotype and changes in gene expression resembling those in patients.

Selective Pyk2 inhibition enhances bone restoration through SCARA5-mediated bone marrow remodeling in ovariectomized mice.
Yunqing Liu; Mai Nishiura; Mika Fujii; Sumiti Sandhu; Yasutaka Yawaka; Yutaka Yamazaki; Akira Hasebe; Tadahiro Iimura; Sek Won Kong; Ji-Won Lee
Cell communication and signaling : CCS, 22, 1, 561, 561, 22 Nov. 2024, [International Magazine]
English, Scientific journal, Understanding the intricate cellular interactions involved in bone restoration is crucial for developing effective strategies to promote bone healing and mitigate conditions such as osteoporosis and fractures. Here, we provide compelling evidence supporting the anabolic effects of a pharmacological Pyk2 inhibitor (Pyk2-Inh) in promoting bone restoration. In vitro, Pyk2 signaling inhibition markedly enhances alkaline phosphatase (ALP) activity, a hallmark of osteoblast differentiation, through activation of canonical Wnt/β-catenin signaling. Notably, analysis of human mesenchymal stem cells through RNA-seq revealed a novel candidate, SCARA5, identified through Pyk2-Inh treatment. We demonstrate that Scara5 plays a crucial role in suppressing the differentiation from stromal cells into adipocytes, and accelerates lineage commitment to osteoblasts, establishing Scara5 as a negative regulator of bone formation. Additionally, Pyk2 inhibition significantly impedes osteoclast differentiation and bone resorption. In a co-culture system comprising osteoblasts and osteoclasts, Pyk2-Inh effectively suppressed osteoclast differentiation, accompanied by a substantial increase in the transcriptional expression of Tnfrsf11b and Csf1 in osteoblasts, highlighting a dual regulatory role in osteoblast-osteoclast crosstalk. In an ovariectomized mouse model of osteoporosis, oral administration of Pyk2-Inh significantly increased bone mass by simultaneously reducing bone resorption, promoting bone formation and decreasing bone marrow fat. These results suggest Pyk2 as a potential therapeutic target for both adipogenesis and osteogenesis in bone marrow. Our findings underscore the importance of Pyk2 signaling inhibition as a key regulator of bone remodeling, offering promising prospects for the development of novel osteoporosis therapies.

Dynamic morphometric changes in the mandibular osteocytic lacunae of ovariectomized rats in response to teriparatide, as revealed by three-dimensional fluorescence analyses: Possible involvement of osteocytic perilacunar remodeling.
Atsuko Nakanishi-Kimura; Aya Takakura; Marie Hoshi-Numahata; Haruhisa Watanabe; Mai Nishiura; Yoshiaki Sato; Ryoko Takao-Kawabata; Tadahiro Iimura
Journal of oral biosciences, 66, 1, 49, 60, Mar. 2024, [International Magazine]
English, Scientific journal, OBJECTIVES: Teriparatide [TPTD; human parathyroid hormone (hPTH1-34)] is an anti-osteoporotic drug with bone anabolic effects. Clinical and preclinical studies have indicated that TPTD has value in oral and maxillofacial bone therapies, including jawbone regeneration, periodontal tissue repair, and the treatment of medication-related osteonecrosis of the jaw. However, it is unclear whether the craniofacial bones respond to TPTD similarly to the axial and appendicular bones. Recent studies showed that TPTD acts on both osteocytes and osteoblasts. This study aimed to characterize distinct craniofacial bone sites, with a focus on morphometric changes in osteocytic lacunae in ovariectomized rats receiving TPTD. METHODS: Conventional bone histomorphometric analyses of mandibular and parietal bone sections were conducted. High-resolution confocal imaging-based three-dimensional fluorescence morphometric analyses of osteocytic lacunae in distinct mandibular and parietal bone sites were conducted. RESULTS: We observed dynamic changes in the morphometric characteristics of osteocytic lacunae specifically in alveolar and other mandibular bone sites upon TPTD administration. CONCLUSIONS: These findings suggest that osteocytes in mandibular bone (specifically, alveolar bone) have unique functional characteristics of osteocytic perilacunar remodeling.

Evaluation of cortical bone remodeling in canines treated with daily and weekly administrations of teriparatide by establishing AI-driven morphometric analyses and GIS-based spatial mapping.
Marie Hoshi-Numahata; Aya Takakura; Atsuko Nakanishi-Kimura; Haruhisa Watanabe; Kentaro Takada; Mai Nishiura; Yoshiaki Sato; Ryoko Takao-Kawabata; Tadahiro Iimura
Bone reports, 19, 101720, 101720, Dec. 2023, [International Magazine]
English, Scientific journal, Larger animal models with a well-developed Haversian system, as observed in humans, are ideal to analyze cortical bone remodeling in pharmacological studies of anti-osteoporosis drugs, although they have some limitations in controlling individual variability in size, weight, age, and number. This study aimed to morphometrically analyze cortical bone remodeling focusing on Haversian canals in dogs using four regimens of TPTD with daily and weekly administrations at lower and higher weekly doses (4.9 μg/kg/week and 19.8 μg/kg/week, respectively) for 9 months. A micro-computed tomography-based analysis showed no significant differences among regimen groups. By establishing artificial intelligence (AI)-driven morphometric analyses and geographical information system (GIS)-based spatial mapping of Haversian canals that does not require confocal microscopy but is possible with more commonly used wide field microscopes, we successfully observed significant morphometric distinctions among regimens applied even in dogs. Our analytical results suggested that the daily higher regimen specifically increased the number of eroded pores creating spaces between existing canals, thus stimulating cortical bone remodeling.

Skeletal-Vascular Interactions in Bone Development, Homeostasis, and Pathological Destruction.
Haruhisa Watanabe; Nako Maishi; Marie Hoshi-Numahata; Mai Nishiura; Atsuko Nakanishi-Kimura; Kyoko Hida; Tadahiro Iimura
International journal of molecular sciences, 24, 13, 30 Jun. 2023, [International Magazine]
English, Scientific journal, Bone is a highly vascularized organ that not only plays multiple roles in supporting the body and organs but also endows the microstructure, enabling distinct cell lineages to reciprocally interact. Recent studies have uncovered relevant roles of the bone vasculature in bone patterning, morphogenesis, homeostasis, and pathological bone destruction, including osteoporosis and tumor metastasis. This review provides an overview of current topics in the interactive molecular events between endothelial cells and bone cells during bone ontogeny and discusses the future direction of this research area to find novel ways to treat bone diseases.

当科における5年間の有病児・障害児(者)の初診時実態調査
西浦 まい; 青山 佳仁; 須貝 壮; 野村 奈央子; 中村 光一; 八若 保孝, 小児歯科学雑誌, 64, 地方会抄録号, 12, 12, Feb. 2026
(公社)日本小児歯科学会, Japanese

細胞外基質ラミニンは褐色細胞腫由来PC-12細胞の脱分化を抑制し,本来の副腎髄質細胞機能を回復する
西浦 まい; 渡辺 陽久; 飯村 忠浩, 日本口腔科学会雑誌, 74, 2, 118, 118, Jul. 2025
(NPO)日本口腔科学会, Japanese

自閉スペクトラム症の精神症状治療薬開発を目指した効率的なドパミン産生性神経細胞分化法の確立
西浦 まい; 八若 保孝, 小児歯科学雑誌, 63, 大会抄録号, 282, 282, Apr. 2025
(公社)日本小児歯科学会, Japanese

「"育児・臨床・研究"-3足の草鞋に挑戦中の歯科医師の道のり」と歯科矯正学への骨粗鬆症治療薬の応用の可能性
星 麻里絵[沼端]; 中西 徳子[木村]; 渡辺 陽久; 西浦 まい; 上野 楓実; 西本 慎之介; 孝口 里侑; 佐藤 嘉晃; 飯村 忠浩, 北海道矯正歯科学会雑誌, 52, 1, 61, 68, Dec. 2024
北海道矯正歯科学会, Japanese

骨形成促進薬PTH製剤は骨粗鬆症モデルラットの顎骨特異的に骨細胞性骨リモデリング調節を行う AI駆動型形態計測による解析
中西 徳子[木村]; 星 麻里絵[沼端]; 渡辺 陽久; 西浦 まい; 飯村 忠浩, Journal of Oral Biosciences Supplement, 2024, [P1, 33], Nov. 2024
(一社)歯科基礎医学会, Japanese

顎関節退行性病変における臨床病態解明とケモカインCCL5の関与
渡辺 陽久; 西浦 まい; 星 麻里絵[沼端]; 中西 徳子[木村]; 飯村 忠浩, Journal of Oral Biosciences Supplement, 2024, [P2, 40], Nov. 2024
(一社)歯科基礎医学会, Japanese

褐色細胞腫の分化誘導療法を基盤とした新規薬物療法の探索
西浦 まい; 渡辺 陽久; 中西 徳子[木村]; 星 麻里絵[沼端]; 八若 保孝; 飯村 忠浩, Journal of Oral Biosciences Supplement, 2024, [P2, 41], Nov. 2024
(一社)歯科基礎医学会, Japanese

AIとGISによる新規形態計測法の確立とイヌ皮質骨リモデリングに対するテリパラチドの薬理作用解明
星 麻里絵[沼端]; 高倉 綾; 中西 徳子[木村]; 渡辺 陽久; 高田 健太郎; 西浦 まい; 佐藤 嘉晃; 高尾 亮子[川端]; 飯村 忠浩, 日本骨形態計測学会雑誌, 34, 2, 76, 77, Nov. 2024
日本骨形態計測学会, Japanese

ゲノムワイド解析による下顎前突症疾患感受性遺伝子の探索と同定
星 麻里絵[沼端]; 梶井 貴史; 中西 徳子[木村]; 渡辺 陽久; 西浦 まい; 上野 楓実; 西本 慎之介; 孝口 里侑; 佐藤 嘉晃; 飯村 忠浩, 北海道歯学雑誌, 45, 12, 15, Sep. 2024
北海道歯学会, Japanese

副甲状腺ホルモン1型受容体作動薬の顎骨への薬理作用と歯科矯正治療への応用
中西 徳子[木村]; 星 麻里絵[沼端]; 上野 楓実; 西本 慎之介; 西浦 まい; 孝口 里侑; 渡辺 陽久; 佐藤 嘉晃; 飯村 忠浩, 北海道歯学雑誌, 45, 16, 21, Sep. 2024
北海道歯学会, Japanese

最新の歯学 「お酒に強い」と「骨が丈夫」の意外な関連性
星 麻里絵[沼端]; 中西 徳子[木村]; 渡辺 陽久; 西浦 まい; 佐藤 嘉晃; 飯村 忠浩, 北海道歯学雑誌, 44, 10, 11, Sep. 2023
北海道歯学会, Japanese

【顎関節の退行性病変におけるケモカインCCL5の役割とバイオマーカーとしての可能性】
渡辺 陽久; 星 麻里絵; 西浦 まい; 丸岡 豊; 北川 善政; 飯村 忠浩, 北海道歯学雑誌, 44, 12, 15, Sep. 2023
北海道歯学会, Japanese

破骨細胞の分化成熟におけるPyk2の役割
西浦 まい; 八若 保孝; 飯村 忠浩; 李 智媛, 日本骨形態計測学会雑誌, 33, 1, 197, 197, May 2023
日本骨形態計測学会, Japanese

当科における5年間の有病児・障害児（者）の初診時実態調査
西浦まい; 青山佳仁; 須貝 壮; 野村奈央子; 中村光一; 八若保孝
第43回日本小児歯科学会北日本地方会大会, 11 Oct. 2025, Poster presentation

自閉スペクトラム症の精神症状治療薬開発を目指した効率的なドパミン産生性神経細胞分化法の確立
西浦まい; 八若保孝
第63回日本小児歯科学会大会, 29 May 2025, Poster presentation

細胞外基質ラミニンは褐色細胞腫由来PC-12細胞の脱分化を抑制し、本来の副腎髄質細胞機能を回復する
西浦まい; 渡辺陽久; 飯村忠浩
第79回NPO法人日本口腔科学会学術集会, 16 May 2025, Poster presentation

褐色細胞腫の分化誘導療法を基盤とした新規薬物療法の探索
西浦まい; 渡辺陽久; 中西(木村)徳子; 星(沼端)麻里絵; 八若保孝; 飯村忠浩
第66回 歯科基礎医学会 学術大会, 03 Nov. 2024, Poster presentation

顎関節退行性病変における臨床病態解明とケモカインCCL5の関与
渡辺陽久; 西浦まい; 星(沼端; 麻里絵; 中西(木村) 徳子; 飯村忠浩
第66回 歯科基礎医学会 学術大会, 03 Nov. 2024, Poster presentation

破骨細胞の分化成熟におけるPyk2の役割
西浦まい; 飯村忠浩; 李 智媛
第43回日本骨形態計測学会, 01 Jul. 2023, Oral presentation

神経・骨連関生体模倣システムの確立と三叉神経・神経分泌による骨形成機構の解明
科学研究費助成事業
31 Jul. 2025 - 31 Mar. 2027
西浦 まい
日本学術振興会, 研究活動スタート支援, 北海道大学, 25K24119