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Nishimura Goro

Research Institute for Electronic Science　Research Center of Mathematics for Social CreativityAssistant Professor

Researcher basic information

■ Degree
  • 1989.6 Doctor of Science, Osaka Univeristy, Osaka University
  • 理学修士, 大阪大学
■ URL
researchmap URLホームページURL■ Various IDs
ORCID IDJ-Global ID■ Research Keywords and Fields
Research Keyword
  • 生体計測学
  • 光計測学
  • Biomedical Optics (1301)
  • 生物物理学
  • Biophysics (4306)
Research Field
  • Nanotechnology/Materials, Optical engineering and photon science
  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering), Measurement engineering
  • Life Science, Biomedical engineering
  • Life Science, Biomaterials
  • Natural Science, Biophysics, chemical physics and soft matter physics
■ Educational Organization

Career

■ Career
Career
  • Apr. 2015 - Present
    Hokkaido University, Research Institute for Electronic Science, Assistant Professor
  • Apr. 2012 - Mar. 2015
    Hokkaido University, Research Institute for Electronic Science, Assistant Professor
  • Oct. 2007 - Mar. 2012
    Hokkaido University, Research Institute for Electronic Science, Assistant Professor
  • Apr. 2007 - Sep. 2007
    Hokkaido University, Research Institute for Electronic Science, Assistant Professor
  • Apr. 1992 - Mar. 2007
    Hokkaido University, Research Institute for Electronic Science, Instructor
  • 1992 - 2007
    Research Associate
  • Jul. 1989 - Apr. 1992
    Hokkaido University, Research Institute for Applied Electricity, Instructor
  • 1989 - 1992
    Research Associate,1989.7 Instructor, Hokkaido University
Committee Memberships
  • Apr. 2013 - Mar. 2015
    応用物理学会分科会日本光学会, 幹事, Society
  • 2009 - 2010
    日本光学会, 幹事, Society
  • 2010
    生物物理学会, 分野別専門委員, Society

Research activity information

■ Papers
■ Other Activities and Achievements
■ Books and other publications
  • 非侵襲・可視化技術ハンドブック
    エヌ・ティー・エス, 2007
  • 測定法シリーズ 、42.蛍光分光とイメージングの手法
    日本分光学会・学会出版センター, 2006
  • Fluorescence spectroscopy and imaging
    2006
  • DOS/V科学計測入門
    HFS出版, 1994
■ Lectures, oral presentations, etc.
  • Sensing and quantifying fluorescence objects in dense scattering medium
    Goro Nishimura
    Sensing and Imaging through Scattering and Fluctuating Field in Biology, Telecommunication, and Astronomy, 22 Apr. 2025, English, Invited oral presentation
    21 Apr. 2025 - 23 Apr. 2025, [Invited]
  • Measurements in Diffuse Optics – Is the interpretation correct?
    Goro Nishimura
    Optics & Photonics Japan 2024, 01 Dec. 2024, Japanese, Nominated symposium
    29 Nov. 2024 - 01 Dec. 2024, [Invited]
  • Fluorescence diffuse optical tomography – ideal and reality of an inverse problem
    Goro Nishimura
    7th Joint Conference of A3 Foresight Program on Computational and Applied Mathematics, 01 Sep. 2024, English, Invited oral presentation
    30 Aug. 2024 - 01 Sep. 2024, [Invited]
  • Near-infrared fluorescence detection for assessment of aspiration risk
    G. Nishimura; T. Suzuki; K. Tashiro; K. Wakamatsu; T. Koike; Y. Yamada; H. Niwa; Y. Michiwaki
    The Third Britton Chance Symposium on Metabolic Imaging and Spectroscopy, 18 Jul. 2024, English, Oral presentation
    17 Jul. 2024 - 20 Jul. 2024
  • Depth detection limit of a fluorescence object in a strongly scattering medium with background emission
    Goro Nishimura; Takahiro Suzuki; Yukio Yamada; Haruki Niwa; Takuji Koike
    Optics & Photonics Japan 2023, 28 Nov. 2023, Japanese, Oral presentation
    27 Nov. 2023 - 29 Nov. 2023
  • Nonlinear concentration-dependent scattering coefficient with a colloidal solution and its origin
    Yuki Inoue; Hiroyuki Fujii; Goro Nishimura
    21th International Conference on Near Ingrared Spectroscopy, 20 Aug. 2023, English, Poster presentation
    20 Aug. 2023 - 24 Aug. 2023
  • Size parameter expansion of the scattering coefficient under the interference effect of a dense scattering medium
    Goro Nishimura; Hiroyuki Fujii; Yuki Inoue
    Optics & Photonics Japan 2022, 16 Nov. 2022, Japanese, Oral presentation
    13 Nov. 2022 - 16 Nov. 2022
  • An analysis of detection angle dependence of diffuse light (II)
    Goro Nishimura
    Optics & Photonics Japan 2022, 16 Nov. 2022, Japanese, Oral presentation
    13 Nov. 2022 - 16 Nov. 2022
  • Angle Dependent Time-domain Diffuse Reflectance – A Simulation Study
    Goro Nishimura
    Sensing and Imaging through Scattering and Fluctuating Field in Biology, Telecommunication, and Astronomy, 21 Apr. 2022, English, Oral presentation
    18 Apr. 2022 - 22 Apr. 2022
  • Diffuse optical imaging - Time-domain approach
    Goro Nishimura
    The 82nd JSAP Autumn Meeting 2021, 10 Sep. 2021, Japanese, Invited oral presentation
    10 Sep. 2021 - 13 Sep. 2021, 29352704, [Invited]
  • Fluorescence diffuse optical tomography with a cuboid target
    Chunlong Sun; Goro Nishimura; Gen Nakamura; Manabu Machida; Jijun Liu; Yu Jang
    Optics and Photonics Japan 2020, 17 Nov. 2020, Japanese, Oral presentation
    14 Nov. 2020 - 17 Nov. 2020
  • Time-domain Fluorescence Diffuse Optical Tomography using a Cuboid
    Chunlong Sun; Goro Nishimura; Gen Nakamura; Manabu Machida; Yu Jiang; Jijun Liu
    Biophotonics Congress: Biomedical Optics 2020, 20 Apr. 2020, English, Poster presentation
    20 Apr. 2020 - 23 Apr. 2020, 29352704
  • High sensitive detection of fluorescence target in biological tissue (3)
    Goro Nishimura
    Optics and Photonics Japan 2019, 05 Dec. 2019, Japanese, Oral presentation
    02 Dec. 2019 - 05 Dec. 2019, 29352704
  • 生体組織中に局在する蛍光体の高感度検出
    西村 吾朗
    第３期第５回「レーザーバイオ医療」技術専門委員会, 26 Jul. 2019, Japanese, Invited oral presentation
    26 Jul. 2019, 29352704, [Invited]
  • 生体組織の光学計測
    西村 吾朗
    「光×質量分析」の可能性, 22 Mar. 2019, Japanese, Poster presentation
    22 Mar. 2019, [Invited]
  • SSensitivity of fluorescence signal in strong scattering medium with background fluorescence
    Goro Nishimura
    A3 Workshop in Applied Inverse Problems, 28 Jan. 2019, English, Invited oral presentation
    28 Jan. 2019 - 30 Jan. 2019, 10748193, [Invited]
  • High sensitive detection of near-infrared fluorescence probes for medical applications
    NISHIMURA Goro
    34th NIR Forum held under Japan Council for Near Infrared Spectroscopy (JCNIRS), 22 Nov. 2018, Japanese, Oral presentation
    [Domestic Conference]
  • High sensitive detection of fluorescence target in biological tissue (2)
    NISHIMURA Goro
    Optics and Photonics Japan 2018, 31 Oct. 2018, Japanese, Oral presentation
    [Domestic Conference]
  • Time-Domain Optical Tomography For Fluorescence Objects
    NISHIMURA Goro
    Tianyuan Workshop on Mathematical and Computational Challenges of Medical Imaging and Inverse Problems, 13 Aug. 2018, English, Nominated symposium
    [Invited], [International presentation]
  • Fluorescence diffuse optical tomography: Time-domain approach
    NISHIMURA Goro
    The 37th JAMIT Annual Meeting, 25 Jul. 2018, Japanese, Invited oral presentation
    [Invited], [Domestic Conference]
  • Fluorescence Image Contrast Improvement by a Time-domain Method
    NISHIMURA Goro
    2018 OSA Biophotonics Congress: Biomedical Optics, 03 Apr. 2018, English, Poster presentation
    [International presentation]
  • Optical tomography for near-infrared fluorescence imaging
    NISHIMURA Goro
    Inverse problems and medical imaging, 13 Feb. 2018, English, Public symposium
    [Invited], [International presentation]
  • Diffuse Correlation Spectroscopy: Analysis of Moving Particles
    NISHIMURA Goro
    Inverse problems and medical imaging, 13 Feb. 2018, English, Public symposium
    [Invited], [International presentation]
  • Temporal response function of multiple scattering light - injection-detection geometry
    NISHIMURA Goro
    Transport Theory in Biomedical Optics, 01 Feb. 2018, Japanese, Public symposium
    [Invited], [Domestic Conference]
  • Optical parameters in Melons by Optical Diffusion Model
    HATTORI K; FUJII H; NISHIMURA G; KOBAYASHI K; WATANABE M
    31st NIR Forum held under Japan Council for Near Infrared Spectroscopy, 27 Nov. 2017, Japanese, Oral presentation
    [Domestic Conference]
  • High sensitive detection of fluorescence target in biological tissue
    NISHIMURA Goro
    Optics and Photonics Japan 2017, 30 Oct. 2017, Japanese, Poster presentation
    [Domestic Conference]
  • Aspiration Risk Detection Using Oral Administratio n of Fluorescent Food -- Preliminary Experiments Using Meat Phantoms
    SUZUKI T; SAITO R; KITADA N; KOIKE T; MAKI S; MICHIWAKI Y; NISHIMURA G; NIWA H; YAMADA Y
    2017 IEE E International Conference on Cyborg and Bionic Systems, 17 Oct. 2017, English, Poster presentation
    [International presentation]
  • Time-domain fluorescence diffuse optical tomography for quantitative imaging of a fluorescence target in deep biological tissue
    NISHIMURA Goro
    The 94th Annual Meeting of the Physiological Society of Japan, 28 Mar. 2017, Japanese, Invited oral presentation
    [Domestic Conference]
  • A novel approach for the time-domain fluorescence imaging of a semi-infinite turbid medium: Monte Carlo evaluation
    PRIETO Kernel; NISHIMURA Goro
    Bios SPIE Photonics West, 28 Jan. 2017, English, Oral presentation
    [International presentation]
  • Quantitative method to determine the optical properties of melons by the photon diffusion equation
    HATTORI K; FUJII H; NISHIMURA G; KOBAYASHI K; WATANABE M
    Asian NIR Symposium 2016, 30 Nov. 2016, English, Oral presentation
    [International presentation]
  • Near-infrared optical properties of white bread using the light propagation model
    FUJII H; NISHIMURA G; HATTORI K; KOBAYASHI K; WATANABE M
    Asian NIR Symposium 2016, 30 Nov. 2016, English, Poster presentation
    [International presentation]
  • Fluorescence diffuse optical tomography for a fluorescence target in a semi-infinite medium
    PRIETO Kernel; NISHIMURA Goro
    Optics and Photonics Japan 2016, 30 Oct. 2016, Japanese, Oral presentation
    [Domestic Conference]
  • Optical properties of melons determined by an analysis based on the photon diffusion equation
    HATTORI K; FUJII H; NISHIMURA G; KOBAYASHI K; WATANABE M
    First Food Chemistry Conference - Shaping the Future of Food Quality, Health and Safety, 30 Oct. 2016, English, Poster presentation
    [International presentation]
  • Near-infrared fluorescence detection and its medical applications
    NISHIMURA Goro
    The 10th ICME International Conference on Complex Medical Engineering, 06 Aug. 2016, English, Invited oral presentation
    [International presentation]
  • A new strategy of the time-domain fluorescence imaging for a semi-infinite turbid media
    PRIETO Kernel; NISHIMURA Goro
    2nd Biomedical Imaging and Sensing Conference 2016, 18 May 2016, English, Oral presentation
    [International presentation]
  • Analysis of the dynamic properties in strong scattering system with DCS and TRS measurements
    NISHIMURA Goro
    Optics and Photonics Japan 2015, 28 Oct. 2015, Japanese, Oral presentation
    [Domestic Conference]
  • A novel fluorescence lifetime analysis for in vivo measurements
    NISHIMURA Goro
    The 14th Conference on Methods and Applications in Fluorescence, 13 Sep. 2015, English, Poster presentation
    [International presentation]
  • Diffuse Optical Tomography - Actual Problems in Reconstruction with Time-domain Data
    NISHIMURA Goro
    The 9th ICME International Conference on Complex Medical Engineering, 18 Jun. 2015, English, Invited oral presentation
    [Invited], [International presentation]
  • Near-infrared fluorescence fluctuation measurement system -- Its design and applications
    NISHIMURA Goro
    5th Asian and Pacific Rim Symposium on Biophotonics, 22 Apr. 2015, English, Poster presentation
    [International presentation]
  • Study on a reconstuction technique using time domain reflectance measurements data
    FURUKAWA Daisuke; NISHIMURA Goro
    5th Asian and Pacific Rim Symposium on Biophotonics, 22 Apr. 2015, English, Oral presentation
    [International presentation]
  • ヒト組織深部のイメージングを可能とする定量的蛍光分子イメージング基盤技術の確立
    西村 吾朗
    第５３回日本生体医工学会大会, 26 Jun. 2014, Japanese, Nominated symposium
    [Invited], [Domestic Conference]
  • Fluorescence decay measurement in tissue-like scattering medium
    NISHIMURA Goro; FURUKAWA Daisuke; AWASTHI Kamlesh
    OSA Topical meeting Biomedical Optics, 26 Apr. 2014, English, Poster presentation
    [International presentation]
  • Tissue optical properties in an 1-um wavelength region
    Nishimura Goro
    The Fifteenth Conference of Peace through Mind/Brain Science, 20 Feb. 2014, English, Invited oral presentation
    [Invited], [International presentation]
  • 時間分解法による生体組織計測へ向けて
    西村 吾朗
    香川大学社会連携・知的財産センター技術協力会, 01 Mar. 2013, Japanese, Public discourse
    [Invited], [Domestic Conference]
  • Non-contact type time-domain fluorescence diffuse optical tomography for quantitative analysis of fluorophores
    Nishimura Goro
    Bios SPIE Photonics West, 02 Feb. 2013, English, Invited oral presentation
    [Invited], [International presentation]
  • 散乱体中での蛍光寿命計測
    西村 吾朗
    日本光学会年次学術講演会 OPJ2012, 23 Oct. 2012, Japanese, Oral presentation
    [Domestic Conference]
  • 蛍光を用いた蛍光物質の吸収の拡散光イメージング ーその考え方と実験データの考察
    西村 吾朗
    日本光学会年次学術講演会 OPJ2011, 28 Nov. 2011, Japanese, Oral presentation
    [Domestic Conference]
  • Complex formation of some near-infrared cyanine dyes in serum albumin - serum albumin induces a new reaction
    Nishimura Goro
    Biophysics Soceity of Japan Annual Meeting, 16 Sep. 2011, English, Oral presentation
    [Domestic Conference]
  • Time-domain fluorescence diffuse optical tomography for living animals by total-light algorithm
    Nishimura Goro
    Bios SPIE Photonics West, 22 Jan. 2011, English, Oral presentation
    [International presentation]
  • 時間領域蛍光イメージング -時間応答関数の性質と定量的イメージング
    西村 吾朗
    日本光学会 (OPJ2010), 08 Nov. 2010, Japanese, Oral presentation
    [Domestic Conference]
  • 1μm波長域での組織光計測
    西村 吾朗
    第14回酸素ダイナミクス研究会, 04 Sep. 2010, Japanese, Nominated symposium
    [Domestic Conference]
  • 1P346 Oxygen concentration measurements by a phosphorescence intensity correlation method(New biophysical techniques and instrumentation,Poster Presentations)
    Nishimura Goro; Pack Chan-Gi; Tamura Mamoru
    Biophysics, 20 Nov. 2007, English
  • 3P314 Scattering and absorption properties in tissues at the 1μm NIR region
    Nishimura G; Tamura M
    Biophysics, 10 Nov. 2004, Japanese
  • Nano-second fluorescence decay measurements by a digital storage oscilloscope
    Nishimura G; Tamura M
    Biophysics, 25 Aug. 2003, Japanese
  • Diffusion dynamics depending on the time scale of observation inhyaluronan aqueous solution
    Masuda Akiko; Ushida Kiminori; Nishimura Goro; Kinjo Masataka; Tamura Mamoru; Koshino Hiroyuki; Yamashita Koichi
    Meeting abstracts of the Physical Society of Japan, 13 Aug. 2002, Japanese
  • Multi photon excitation fluorescence correlation spectroscopy - measurement of dye molecules in UV region
    Nishimura G; Kinjo M
    Biophysics, 05 Aug. 2000, Japanese
  • The cleavage of dsDNA by restriction endonucleases monitored by flourescence correlation spectroscopy
    Nishimura G; Kinjo M
    Biophysics, 02 Sep. 1999, Japanese
  • Fluorescence Correlation Spectroscopy as an Analytical Tool of Enzymatic Reactions in the Single Molecule Level
    NISHIMURA Goro; KINJO Masataka
    Biophysics, 25 Mar. 1999, Japanese
    Fluorescence correlation spectroscopy (FCS) method has been attractive as an analytical tool, particularly in biophysics and biochemistry, with the recent developments of the method. FCS with confocal optics of microscope and real time correlator equipment achieves analysis of fluorescent species in single molecu1e level from a very small sample area (<1 mm) and volume (〜fl) in almost real time (sec〜min). An application to the analysis of enzymatic reaction in solution with FCS is demonstrated as a basis of the analysis to tackle the problems in vivo. The concept of FCS and future view are also discussed.
  • 29p-XF-7 Fluorescence correlation analysis of cleavage reaction of dsDNA by restriction enzymes
    Nishimura G; Kinjo M
    Meeting abstracts of the Physical Society of Japan, 15 Mar. 1999, Japanese
  • Analysis of Gelation by Deffusing-Wave Spectroscopy
    NISHIMURA G; WEITX D.A; YODH A.G
    Meeting abstracts of the Physical Society of Japan, 10 Mar. 1998, Japanese
  • 2Q18 Analysis of interactions between Chaperon GroEL and substrates using Fluorescence Correlation Spectroscopy
    Pack C; Nishimura G; Kinjo M; Tamura M; Aoki K; Taguchi H; Yoshida M
    Biophysics, 05 Sep. 1997, Japanese
  • 29p-WE-3 Analysis of Interactions between Chaperonin GroEL and Substrates using Fluorescence Correlation Spectroscopy(FCS)
    Pack C; Nishimura G; Kinjo M; Aoki K; Taguchi H; Yoshida M
    Meeting abstracts of the Physical Society of Japan, 17 Mar. 1997, Japanese
  • 自己相関蛍光分光法を用いたDNA断片数の分子カウント
    金城 政孝; 西村 吾朗; 小山 富康
    日本分子生物学会年会プログラム・講演要旨集, 01 Aug. 1996, Japanese
  • FCS Measurements in Hydrolysis Process of Long dsDNA
    Nishimura Goro; Kinjo Masataka
    Abstracts of the meeting of the Physical Society of Japan. Sectional meeting, 12 Sep. 1995, Japanese
  • Measurement of oxygen metabolism in living tissue by time-resolved spectrophotometry.
    ODA MOTOKI; YAMASHITA YUTAKA; NISHIMURA GORO; TAMURA MAMORU
    応用物理学会学術講演会講演予稿集, Aug. 1995, Japanese
  • Quantitative Analysis of Absorption in Turbid Media by photon Correlation method.
    NISHIMURA GORO; KATAYAMA KAORU; KINJO MASATAKA; TAMURA MAMORU
    日本生物物理学会年会講演予稿集, Aug. 1995, Japanese
  • Quantitative Measurement of Hemoglobin in living tissue by time-resolved spectrophotometry.
    ODA MOTOKI; YAMASHITA YUTAKA; OTA KAZUYOSHI; NISHIMURA GORO; TAMURA MAMORU
    応用物理学関係連合講演会講演予稿集, Mar. 1994, Japanese
  • Quantitative analysis of light absorption in living tissue by time-resolved spectrophotometry.
    小田元樹; 山下豊; 太田和義; 西村吾朗; 田村守
    応用物理学会学術講演会講演予稿集, Sep. 1993, Japanese
  • 30a-D-13 Time-Resolved Fluorescence of NADH (IV)
    Wakita M; Nishimura G; Tamura M
    年会講演予稿集, 12 Mar. 1992, Japanese
  • 30a-D-12 Time-Resolved Fluorescence of NADH (III)
    Nishimura G; Wakita M; Tamura M
    年会講演予稿集, 12 Mar. 1992, Japanese
  • 3p-C3-7 結晶中Eu^<3+>の蛍光の圧力効果
    西村 吾朗; 栗田 厚; 櫛田 孝司
    秋の分科会講演予稿集, 16 Sep. 1988, Japanese
  • 3a-G3-12 タンパク中トリプトファンの蛍光寿命の温度依存性(生体物理)
    西村 吾朗; 栗田 厚; 櫛田 孝司
    年会講演予稿集, 18 Mar. 1988, Japanese
  • 28p-D-14 Eu^<3+>プローブによるアモルファス物質中の結晶場解析
    西村 吾朗; 櫛田 孝司
    秋の分科会講演予稿集, 16 Sep. 1987, Japanese
  • 1a-D5-7 Ca(PO_3)_2:Eu^<3+>ガラスの蛍光スペクトルの偏光特性(1a D5 イオン結晶・光物性(不純物中心・欠陥生成・磁性体・他),イオン結晶・光物性)
    西村 吾朗; 櫛田 孝司
    年会講演予稿集, 31 Mar. 1985, Japanese
  • 5p-TC-4 Ca(PO_3)_2ガラス中でのEu^<3+>のエネルギー準位
    西村 吾朗; 櫛田 孝司
    秋の分科会講演予稿集, 10 Sep. 1984, Japanese
■ Affiliated academic society
  • SPIE
  • 日本光学会
  • 米国光学会
  • 物理学会
  • 生物物理学会
  • Optical Soceity of Japan
  • Optical Society of America
  • The Biophysical Society of Japan
  • The Physical Society of Japan
■ Research Themes
  • Monitoring of residual food in hypopharynx using near-infrared fluorescence: aspiration risk detection and treatment with electrical stimulation
    Grants-in-Aid for Scientific Research
    Apr. 2024 - Mar. 2027
    小池 卓二; 西村 吾朗; 中川 量晴; 香取 幸夫; 森本 韻
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), The University of Electro-Communications, 24K03305
  • Study on the transport of topological lightwave in dense scattering media for an advanced fluorescence diffuse optical tomography
    Grants-in-Aid for Scientific Research
    Apr. 2023 - Mar. 2025
    西村 吾朗
    拡散蛍光トモグラフィにおける再構成画像を改善するために蛍光検出の感度、蛍光の時間応答関数の数学的解析、さらに励起光の散乱体透過深度向上のための光渦の散乱体透過特性に関して検討した。
    最初に、過去行った生体組織模擬試料に埋め込まれた蛍光体の測定データを解析するために解析モデルを構築した。検出深さ感度を定量的に評価するために測定データの分散に注目し、それに対する蛍光信号の大きさを持って評価した。その結果、外来光の存在下では外来光に基づく検出光ゆらぎが検出可能深さを決定している主なる原因であることがわかった。それをもとに入射受光間距離の最適値を評価し、結果を論文としてまとめ投稿中である。
    蛍光の時間応答関数を用いた画像再構成において、時間応答関数を特徴づけるパラメータとして何を用いるべきかと言う議論があるが、最もノイズレベルが低くなる時間応答関数の最大値を与える時間(ピーク時間)がロバストなパラメータである。そこで、蛍光体を点とした時のピーク時間と蛍光体の位置に関する関係を数学的に解析した。蛍光の時間応答関数は励起光蛍光それぞれの光伝搬関数の畳込み積分で表現されるが、それからピーク時間を求めるために蛍光体深さを十分深いと考える極限で考え、界面のFresnel反射を考慮した新しい解析式の導出に成功しその結果を論文として発表した(Eom et.al. 2024, Shuli et.al.2023)。また、蛍光寿命を考慮した解析式の導出の試みを進め、現在投稿論文としてまとめている段階である。
    光渦の散乱体伝搬特性が異なることがこれまで報告されているが、その透過特性を確かめるために液晶retarderを利用したLaguerre-Gaussモードの生成光学系を構築し、イントラリピッドを散乱体とする溶液での散乱透過パターンを計測した。その結果は報告結果と異るため詳細な検討を進めている。
    Japan Society for the Promotion of Science, Grant-in-Aid for Transformative Research Areas (A), Hokkaido University, 23H04127
  • Influence of the interference effects on radiative transfer in dense colloidal suspensions
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    01 Apr. 2020 - 31 Mar. 2023
    藤井 宏之; 西村 吾朗; 渡部 正夫; 小林 一道
    本研究の目的は、「生体を模擬した高密度なコロイド溶液に対して、輻射（ふくしゃ）の干渉効果を組み込んだ輻射輸送モデル(輸送モデル)を新たに構築した上で、干渉効果が及ぼす光伝播への影響を明らかにすること」である。生体光イメージングや光治療において、生体内の光伝播を定量的に把握することが必要であり、光を輻射と捉えた輸送モデルが活用されている。しかし、従来の輸送モデルには、生体のような高密度な散乱媒体において重要となる干渉効果が考慮されていない。本研究では電磁波の散乱理論より干渉効果を考慮した散乱特性を計算し、輸送モデルに組み込んだ上で光強度の時間分解波形を数値計算する。また、短パルスレーザー光源による光強度計測も実施する。干渉効果が光伝播へ影響を及ぼす時空間スケールについて、散乱係数などで表される無次元数を用いて解析し、理論、数値計算、実験より体系的に明らかにする。この目的を達成するため、本年度は以下の３項目について研究を実施した。
    １．光散乱特性を簡潔に記述するモデル式を開発した。モデル式により、単一散乱の寄与と干渉効果の寄与を分離して評価することに初めて成功した。
    ２．分担研究者の西村吾朗助教の波長可変の光計測システムを利用して、コロイド溶液（脂質エマルジョン）において、濃度20%まで21条件、720 nmから860 nmまでの6条件で光計測を実施した。光計測データを逆解析することで算出した光学特性値をモデル式より解析し、干渉効果の波長依存性を明らかにした。
    ３．分子動力学シミュレーションにより構造特性を計算し、干渉散乱理論に組み込んで散乱特性を計算した。粒子間の引力相互作用が散乱特性に強く影響を及ぼすことを明らかにした。
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 20H02076
  • 近赤外蛍光検査システムを応用した新しい嚥下機能評価に関する基礎的研究
    科学研究費助成事業 基盤研究(C)
    01 Apr. 2020 - 31 Mar. 2023
    吉見 佳那子; 小池 卓二; 道脇 幸博; 西村 吾朗; 戸原 玄; 中川 量晴
    本年度は、まず、蛍光計測法の有用性評価のための予備実験を実施した。
    牛肉ブロックとバルーンカテテールを用いてヒトの咽頭の梨状窩を再現し作成したファントムと、ヤギの頭頸部標本を使用した。実験項目は、①近赤外線ファイバおよび蛍光検出ファイバを取り付けたプローブの頚部への照射角の設定、②咽頭通過する食物中の蛍光を計測可能な蛍光強度積算時間の設定、③咽頭残留を計測する最適スパンの検討、である。その結果、①光プローブと頚部の接触角度が蛍光強度可否に大きく影響すること、②嚥下時に食塊が咽頭を通過する速度での蛍光検出は深さ25mmまでは可能であること、③最適スパンは10mmから20mmであること、がわかった。
    また、人を対象とした計測では蛍光計測と嚥下造影検査を同時に実施する予定であったため、ICG入り検査食に硫酸バリウムやとろみ剤を混和した場合に、蛍光強度に影響するか調査した。その結果、混和前と比較し混和後は蛍光強度が大きくなる傾向が見られたが、検査自体には支障はないことがわかった。
    予備実験終了後、実際に嚥下障害患者を対象とし計測を実施した。対象は、東京医科歯科大学病院摂食嚥下リハビリテーション外来を受診する嚥下障害患者4名である。嚥下時の蛍光計測および嚥下造影検査を同時に実施し、得られた蛍光強度と検査画像を対応させた。その結果、咽頭残留、咽頭通過の蛍光検出が可能であった。一方で、プローブの接触位置や接触強さが検出精度に影響するため、プローブ形態やセンサの感度を改良し、検査手技の簡易化と検出精度向上を検討する必要があることが示唆された。
    日本学術振興会, 基盤研究(C), 東京医科歯科大学, 20K12741
  • A high-sensitive time-domain method to identify fluorescence targets in thick biological tissue
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    01 Apr. 2019 - 31 Mar. 2022
    西村 吾朗; 藤井 宏之
    散乱体に埋め込まれた蛍光体に関して、その時間応答関数に関して、研究協力者と検討した。特に、S/Nが最も良くなる時間応答関数が最大値を取る時間を、点蛍光体に関して解析的に導出し、厳密な証明を与えた。これらの結果を投稿論文としてまとめ投稿した。
    時間応答関数を観測する上で、検出条件が与える影響を評価するために、MCシミュレーションを行った。特に、検出方向の境界面に対する角度依存性を検討した。検出方向の方位角を4分割、極角を10分割しそれぞれの方向で検出される光子数に関してシミュレーションを行った。入射点と検出点を結ぶ方向に対し直角となる方位角で検出する場合の極角に対する依存性は、界面のフレネル反射と拡散反射のみで説明可能であった。一方、入射点と検出点を結ぶ方向では、入射点方向から離れる方向では強度が大きくなる一方、逆の方向では強度が小さくなり、直角方向のそれとの違いはほぼ対称的であった。この結果は、拡散近似に含まれる流れの項で説明できることがわかった。その一方、時間応答の速い時間ではそれらからずれ、拡散近似の破れが現れた。これらの結果は、10月に行われた国内学会、4月に行われた国際会議で報告した。
    散乱体濃度に対して時間応答特性がどのように変化するか、特にその波長依存性に関して、共同研究者とより詳細に解析を進めた。その結果、濃度に対する散乱係数の依存性の非線形性が、波長が長くなるにつれより薄い濃度で現れることが明らかになった。
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 19K04421
  • Study of noninvasive aspiration detection using fluorescence food
    Grants-in-Aid for Scientific Research Challenging Research (Exploratory)
    29 Jun. 2018 - 31 Mar. 2020
    YAMADA YUKIO
    For the purpose of evaluating pulmonary aspiration, we have developed a technology to inspect food residue in the piriform sinus noninvasively and easily. When fluorescence food is remained in the piriform sinus, it can be detected by an optical probe attached to the skin for measuring fluorescence emitted from the food. Selection of fluorescence agent emitting near-infrared light with high penetration depth, development of an instrument having high sensitivity to near-infrared fluorescence as well as an optical probe, preliminary experiments using phantoms, and measurements for healthy elderly subjects were conducted.
    It was confirmed that fluorescence from the depth of about 25 mm was detectable, and fluorescence from fluorescence food remained in the piriform sinus of healthy elderly subjects were detected. Consequently, we have established the fundamental technology for inspecting food residue in the piriform sinus noninvasively and easily.
    Japan Society for the Promotion of Science, Challenging Research (Exploratory), The University of Electro-Communications, 18K18448
  • ヒト組織深部のイメージングを可能とする定量的蛍光分子イメージング基盤技術の確立
    産学共創基礎基盤研究
    Dec. 2011 - Mar. 2017
    西村 吾朗
    JST, Principal investigator, Competitive research funding
  • Diffuse Correlation Imaging for Diagnosis of Biological Tissue
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    01 Apr. 2012 - 31 Mar. 2015
    NISHIMURA Goro
    The project aimed to establish a diagnosis technology by means of diffuse correlation imaging. In the project, a multi-point high time-resolution photon counting system was constructed for a multiple correlation analysis of the scattering photon through biological tissue. In addtion, a time-resolved system for the scattering photon was combined to determin optical parameters such as the absorption and scattering coefficients. The system was successifully applied for measurements of phantom and rat abnodominal tissue, yielding the scattering, absorption coefficients and correlation time, which carries the information of tissue structure, blood absorption and blood flow, respectively. Finally, the 2D image could be obtained to visualize these parameters. This novel technology will extend current optical methods for diagnosis of biological tissue.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Principal investigator, Competitive research funding, 24500504
  • リアルタイム分光イメージングによる食品の安全性モニタリングおよび通電加熱による高効率殺菌技術の開発
    重点地域研究開発推進プログラム、育成研究
    Apr. 2009 - Mar. 2012
    澤山 一博
    JST, Competitive research funding
  • 分子イメージング機器研究開発プロジェクト／新規悪性腫瘍分子プローブの基盤技術開発
    新規悪性腫瘍分子プローブの基盤技術開発
    Jan. 2009 - Mar. 2010
    西村 吾朗
    NEDO, Principal investigator, Competitive research funding
  • 近赤外光を用いた脳浮腫モニタリング手法の確立
    シーズ発掘試験
    Aug. 2007 - Mar. 2008
    西村 吾朗
    本研究は光学的手法により脳浮腫を早期にモニタ可能とする技術を確立することが目的である。1μmを超える近赤外光領域を利用し浮腫形成を反映する組織の水の吸収および散乱特性を主として時間分解計測法を用いて評価する。これらのために高感度計測法を検討するとともに、小動物モデルを用いた組織の光学特性評価を行う。それにより、実用化に適した波長などの光学測定条件などを決定し、モニタリング手法の基礎技術を確立する。この結果をさらに、今までのMRIと異なるベッドサイドなどでのモニタが可能となるシステムへと展開することができる。
    JST, Principal investigator, Competitive research funding
  • Development of the multi-point fluorescence correlation spectroscope and analysis of molecular interaction in living cell
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    2003 - 2005
    KINJO Masataka; TAMURA Mamoru; NISHIMURA Goro; MIYAZAKI Tadaaki; NOMURA Yasutomo
    Different distribution of many materials, pH, ion, or oxygen concentration can be detected in living cell according to the different position in the cell such as near or away from plasma membrane, nucleus, endsome or mitochondrion. Microenvironment of the cell is also changed according to physiological condition of the cell. The purpose of the project was construction of a multi-point measurement system of Fluorescence Correlation Spectroscopy that could detect number concentration of fluorescent molecule with the single molecule sensitivity and basic research for the detection of dynamic molecular interaction in a living cell.
    At the first step of the project, we attempted to construct proto type system that could detect cross correlation function using two different fluorescence intensities. Then, detection area was increased to four positions. Finally, we reduced the duration time least 10m sec between moving position to position.
    Glucocorticoid receptor, that is one of transcriptional regulator protein and traslocating protein from cytosol to nucleus after stimulated by TPA, was selected in the project. We detected the increase number of GR-GFP in nucleus and the decrease that in cytosol by TPA stimulation. The diffusion time and the fluorescence intensity per molecule of GR were analyzed using our system (MP-FCS). Furthermore, we carried out the measurement of spacial correlation analysis between two detection areas because the system could detect fluorescence emission from two positions simultaneously. When the molecules move same direction, we will be able to detect increase of correlation amplitude in image correlation function. By using this system, the dynamic movement of biomolecule will be analyzed in living cell.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 15370062
  • DEVELOPMENT OF FLUORESCENCE CORRELATION SPECTROSCOPY SYSTEM AIM AT MOLECULAR DIAGNOSIS FOR MASS EXAMINATION
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    2000 - 2002
    KINJO Masataka; ISHIDATE Fumiyoshi; NOMURA Yasutomo; NISHIMURA Goro; HORI Kunio
    PCR (polymerase chain reaction) and modified PCR method, such as RT-PCR (reverse transcriptase) have been used for detection of specific gene and genomic information. However, conventional PCR analysis contains many processes such as enzymatic reaction, product separation and determination.
    Asymmetric PCR, in which the concentrations of primers are unequal, was introduced to make a single-strand DNA. We focused not on a single-stranded DNA (ssDNA) but on another product, a double-stranded one (dsDNA). When the concentration of the fluorescence labeled primer is lower than that of of the other primer in amplification, fluorescent dsDNA and non-fluorescent ssDNA can be expected as products of the reaction. Under ideal conditions, all of the fluorescence-labeled primer (low-concentration primer) is incorporated into dsDNA after the proper number of PCR cycles. From the viewpoint of fluorescence molecules, short ssDNA (fluorescent primer) is elongated into long dsDNA (fluorescent amplified DNA) during the FA-PCR (fluorescence-labeled primer based asymmetric PCR).
    We determined the product of FA-PCR by FCS (Fluorescence Correlation Spectroscopy) under homogenous condition. FCS provides the average number of molecules in the volume element and the diffusion constant of the molecule.
    In this project we determined the yield of several kind of DNA in FA-PCR using FCS as a simple tool for detection of specific genome and sequence.
    We reported the sensitivity and simplicity of FCS in detecting dsDNA in FA-PCR without another extra specific probe. The results indicated the combination of FCS and FA-PCR could detect the target gene at the single copy level at the initial concentration in 25 micro litter solution without using any separation or purification method such as gel electrophoresis.
    We also developed FA-PCR detection system based FCS. The system consisted with sample carrier stage and data evaluation computer system.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), HOKKAIDO UNIVERSITY, 12557235
  • MONITORING OF INTRACELLULAR OXYGEN -CONCENTRATION DURING HYPOXIA INDUCIBLE FACTOR ACTIVATION USING OXYGEN PHOSPHORECSENCE QUENCHING
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    2000 - 2001
    NOMURA Yasutomo; NISHIMURA Goro
    MAMMALIAN CELLS ARE CRITICALLY DEPENDENT ON OXYGEN FOR SURVIVAL. THE MOLECULAR MECHANISMS BY WHICH CELLS SENSE AND RESPOND TO A REDUCTION IN OXYGEN SUPPLY ARE NOT KNOWN ALTHOUGH FINDINGS ON THE SIGNAL TRANSDUCTION PATHWAY HAVE BEEN REPORTED BY SEVERAL AUTHORS. A TYPICAL EXAMPLE IS THAT HYPOXIA STIMULATES TRANSCRIPTION OF A NUMBER OF GENES CRUCIAL TO SURVIVAL IN THE HYPOXIC STATE, AS WELL AS APOPTOSIS. THE ENHANCEMENT OF TRANSCRIPTION OF SOME GENES BY HYPOXIA IS MAINLY MEDIATED BY HYPOXIA-INDUCIBLE FACTOR-1 (HIF-1). HOWEVER, IT IS NOT CLEAR WHETHER THIS TRANSCRIPTION FACTOR ARE ACTIVATED BY HYPOXIA-INDUCED CHANGES IN ENERGY METABOLISM OR BY OXYGEN SHORTAGE DIRECTLY THROUGH AN OXYGEN SENSOR. TO ANSWER THIS, WE ATTEMPTED TO CORRELATE THE GENE-EXPRESSION AND INTRACELLULAR OXYGENATION STATE.
    GREEN FLUORESCENT PROTEIN (GFP) FROM AEQUOREA VICTORIA IS A POWERFUL FLUOROPHORE FORMED AUTOCATALYTICALLY BY INTERNAL CYCLIZATION AND OXIDATION OF THE SER-TYR-GLY SEQUENCE AT POSITIONS 65-67 WITHIN THE 238 AMINO ACID. FOLLOWING THE CLONING OF THE CDNA FOR GFP, SEVERAL INVESTIGATORS TOOK A GREAT INTEREST IN THE EXPRESSION OF GFP AND GFP-FUSION PROTEINS AS A UNIQUE FLUOROPHORE TO MONITOR A RANGE OF INTRACELLULAR PROCESSES, ESPECIALLY THE LOCALIZATION OF PROTEINS. HIF- 1 IS THE TRANSCRIPTION REGULATORY PROTEIN AND TRANSLOCATE FROM THE CYTOPLASM TO THE NUCLEUS DURING HYPOXIA. WE OBSERVED THE NUCLEAR TRANSLAOCATION OF GFP LABELED HIF-1 BY COBALT TREATMENT WHICH MIMICS HYPOXIA.
    OXYGEN-DEPENDENT QUENCHING OF PHOSPHORESCENCE IS A FUNCTION OF THE FREQUENCY OF COLLISION BETWEEN THE PHOSPHOR AND MOLECULAR OXYGEN AND OF THE EFFICIENCY OF ENERGY TRANSFER DURING THESE COLLISMNS. WE DEVELOPED A DETECTION SYSYTE FOR PALLADIUM PORPHYRIN PHOSPHORESCENCE LIFETIME USING CONFOCAL MICROSCOPE. USING PHOSPHORESCENCE DECAY CONSTANT PREVIOUS REPORTED, THE OXYGEN CONCENTRATIONS IN THE MEDIUM WERE ESTIMATED AS 194 MICROMOLAR UNDER NORMOXIA AND 29 MICROMOLAR UNDER ANOXIA, RESPECTIVELY. WE PLAN TO DETERMINE OXYGEN GRADIENT BETWEEN CYTOSOL AND MEDIUM.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), 12680821
  • ANALYSIS OF INTERACTION BETWEEN DNA STRANDS IN TRIPLE HELIX USING FLUORESCENCE CROSS CORRELATION SPECTROSCOPY.
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    1999 - 2000
    KINJO Masataka; NISHIMURA Goro
    The purpose of this research is to clarify the characteristic interaction between DNA strands in triple DNA helix by using fluorescence cross correlation spectroscopy (FCCS) method.
    The use of autocorrelation analysis in fluorescence correlation spectroscopy (FCS) to monitor hybridization open new degree of sensitivity in the nucleic acid research, however the mass ratio of target strand must be excess about 8 times bigger than labeled strand.
    Here we try to use dual color correlation method, fluorescence cross correlation spectroscopy, for detection the interaction between same length of double strand and single strand.
    Materials and Method
    Cy5- and Rhodamine Green (RG) labeled DNAs of 40 base length, and 20 base were used in this experiment as a model system.
    Measurements were carried out with the concentration of 20 base, 40 base and control were 2x10-8M, 10-9M and 10-8M, respectively. FCS measurements were carried out wiht ConfoCor 2 (zeiss) with measurement timeof 40 sec.
    Results
    The translational diffusion time of both strands (plus and minus) is not sodifferent each other because the length of each strand is equal so that detection of the hybridization product (double strand) by using autocorrelation method (FCS) is very difficult. However, by using the calculation of cross correlation signals from FCCS between both Cy5- and RG, the process of hybridization was detected in a homogeneous solution without any physical separation method. Yield of double strand DNA was calculated about 50% for 40bp and 45% for 20bp with equation. On the other hand, in the case of hybridization between 40 base and 20 base, cross correlation signal was not detected because no complimentary sequence is contained. We now try to detect the formation of triplex DNA in solution by the new FCCS method.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), HOKKAIDO UNIVERSITY, 11680651
  • STUDY FOR MECHANISM OF TRIPLEX USING SINGLE MOLECULES DETECTION
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
    1995 - 1996
    KINJO Masataka; NISHIMURA Goro
    PURPOSE :
    The formation process of triple helix DNA in an aqueous solution was analyzed using Fluorescence Correlation Spectroscopy in tiny volume at the single molecule detection. The triple helix DNA was made with fluorescence labeled oligo T15 as a probe DNA strand and homo purin : homo pyrimidine DNA as a target strand. Changing the translational diffusion time of probe DNA was used as indicator for the rate of producing of triplex.
    We confirmed the FCS could be used as determination method for triplex in the first budget year, and we have applied the FCS method to analysis the formation of triplex in solution at the second budget year.
    METHODS :
    Various length of fluorescence labeled DNA (50bp to 7000bp) was synthesized using PCR method in the presence of fluorescence tagged dUTP (Rho-dUTP). The translational diffusion time of these DNA were measured with FCS and had been used as standard. In order to compare with the formation process of triplex, we measured the associate process of T15 to complementary strand DNA.
    CONCLUSION :
    The translational diffusion time of double strand DNA agreed with the theoretical values for a rod-shaped model. We could analyze the translational diffusion time of triplex DNA using same method with two components model since triplex has more rigid structure than double strand DNA.The results show the formation rate of triplex was slower than that of double helix. Also, about 80% of probe DNA bind to target double strand DNA while all of added probe DNA strand bind to target DNA in the case of double strand DNA.
    The experiment with mismatch sequences in the target and/or the probe DNA will be analyzed in future.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), HOKKAIDO UNIVERSITY, 07808072
  • Tissue imaging using near-infrared light
    Competitive research funding
■ Industrial Property Rights
  • 解析装置、撮像システムおよび解析方法
    Patent right, 西村 吾朗; 大舘 暁; 鳥羽 英光; 大滝 桂; 山崎 康子; 菅原 貴光
    特願2017-199471, 13 Oct. 2017
  • 生体検査装置及び生体検査方法
    Patent right, 西村 吾朗
    特願2017-181583, 21 Sep. 2017
  • Arrangement and method to apply diffusing wave spectroscopy to measure the properties of multi-phase systems, as well as the changes terein (AE,AL,AM,AT,AU,AZ,BA,BB,BG,BR,BY,CA,CH,CN,CU,CZ,DE,EE,ES,FL,GB,GD,GB,GH,GM,HR,HU,ID,IL,IN,IS,JP,KE,KG,KP,KR,KZ,・・・
    Patent right
    WO99/51954
    Arrangement and method to apply diffusing wave spectroscopy to measure the properties of multi-phase systems, as well as the changes terein (AE,AL,AM,AT,AU,AZ,BA,BB,BG,BR,BY,CA,CH,CN,CU,CZ,DE,EE,ES,FL,GB,GD,GB,GH,GM,HR,HU,ID,IL,IN,IS,JP,KE,KG,KP,KR,KZ,LC,LK,LR,LS,LT,LU,LV,MD,MG,MK,MN,MW,MX,NO,NZ,PL,PT,RO,RU,SD,SE,SG,SI,SK,SL,TJ,TM,TR,TT,US,UG,US,UZ,YN,YU,ZA,ZW)
  • 散乱媒質中における吸光物質の濃度測定方法
    Patent right
    特開平9-33433
  • 散乱媒質内吸光物質の濃度測定方法および装置
    Patent right
    特許第3310390号
■ Academic and Social Contribution Activities/Other
Social Contribution Activities
  • 光で生体組織を見る!
    25 Oct. 2016
    Lecturer
    北海道新聞社
    国民との科学・技術対話
  • 光と生体観察・計測
    22 Jan. 2015 - 23 Jan. 2015
    Lecturer
    日本光学会
    第５１回冬期講習会