Research activity information

• May 2026, 一般社団法人 歯科基礎医学会, 2026年度（第38回）学会奨励賞
• May 2026, 北海道歯学会, 令和8年度 北海道歯学会賞
• Sep. 2024, 第10回北海道大学部局横断シンポジウム, ベストポスター賞
• Aug. 2024, 第19回 先端歯学スクール(先端歯学2024), 優秀賞
• Aug. 2024, 第35回 日本臨床口腔病理学会総会・学術集会, 優秀ポスター賞

• Functional analysis of miR-199a-3p in tumor endothelial cells
  Yuying Hong; Li Yu; Ikuko Takahashi; Nako Maishi; Aya Matsuda; Yuya Sakurai; Yasuhiro Hida; Ikuya Miyamoto; Kyoko Hida
  Cell Structure and Function, Japan Society for Cell Biology, Feb. 2026, [Peer-reviewed]
  Scientific journal
• SARS-CoV-2 uptake and inflammatory response in senescent endothelial cells are regulated by the BSG/VEGFR2 pathway
  Yuya Sakurai; Yoichiro Fujioka; Nako Maishi; Ryo Takeda; Yusuke Ohba; Michihito Sasaki; Takahito Teshirogi; Wataru Ito; Yasuhiro Hida; Aya Matsuda; Kanta Kido; Yasuko Orba; Hirofumi Sawa; Kyoko Hida
  Proceedings of the National Academy of Sciences of the United States of America, 122, 31, Proceedings of the National Academy of Sciences, 28 Jul. 2025, [Peer-reviewed], [Lead author]
  Scientific journal, Aging is a risk factor for severe COVID-19, characterized by vascular endothelial dysfunction. Although possible susceptibility of vascular endothelial cells (ECs) to SARS-CoV-2 infection has been suggested, the details of entry into cells have not been clarified. Previously, we reported that in an aged mouse model of severe COVID-19, ECs show a massive viral uptake and inflammatory response. Here, we focused on the endocytic capacity of senescent ECs. We found that the senescent ECs showed high endocytic capacity and SARS-CoV-2 virus uptake. This triggers an nuclear factor-kappa B (NF-κB) pathway–mediated inflammatory response. Further, Basigin enhanced endocytosis in the senescent ECs by activating the intracellular vascular endothelial growth factor signaling. Thus, EC senescence is associated with enhanced SARS-CoV-2 endocytosis and subsequent vascular endothelial dysfunction. This could prove a potential target for treating severe COVID-19 in older adults.
• SARS-CoV-2 infection promotes lung thrombosis by inducing integrinβ3 expression in vascular endothelial cells
  Wataru Ito; Yuya Sakurai; Nako Maishi; Ryo Takeda; Takahito Teshirogi; Li Yu; Yasuhiro Hida; Michihito Sasaki; Yasuko Orba; Takuya Tsumita; Haruhisa Watanabe; Tadahiro Iimura; Terufumi Kubo; Shinsuke Toba; Akihiko Sato; Aya Matsuda; Daisuke Kyuno; Makoto Osanai; Yoichi Ohiro; Toshihiko Torigoe; Hirofumi Sawa; Kyoko Hida
  Scientific Reports, 15, 1, Springer Science and Business Media LLC, 01 Jul. 2025, [Peer-reviewed], [Lead author]
  Scientific journal
• Vascular Inflammation and Cancer Malignancy
  Yuya Sakurai; Li Yu; Aya Matsuda; Nako Maishi; Kyoko Hida
  Journal of Oral Biosciences, 100671, 100671, Elsevier BV, May 2025, [Peer-reviewed], [Lead author]
  Scientific journal
• Hypothyroidism Revealed During General Anesthesia in a Patient With Treatment-Resistant Depression: A Case Report
  Makiko Shibuya; Yuya Sakurai; Yukifumi Kimura; Takayuki Hojo; Saori Tada; Toshiaki Fujisawa; Kanta Kido
  Anesthesia Progress, 72, 1, 28, 32, American Dental Society of Anesthesiology (ADSA), Mar. 2025, [Peer-reviewed]
  Scientific journal, General anesthesia in patients with undiagnosed hypothyroidism can lead to neurological, pulmonary, and cardiovascular complications. We report a case of hypothyroidism in a patient with treatment-resistant depression detected intraoperatively based on multiple clinical findings. A 49-year-old woman was scheduled for orthognathic surgery for mandibular prognathism. She had depression since the age of 36 and was taking multiple psychotropic medications. After induction, she had persistent hypotension, bradycardia, low bispectral index, and hypothermia that were resistant to treatment. After ruling out common causes and reducing the anesthetic agents, blood tests were performed intraoperatively to examine thyroid function which revealed hypothyroidism. No delayed recovery or postoperative abnormalities were observed. Lithium carbonate was identified as the most likely cause of hypothyroidism by an endocrinologist. Given the overlap in signs and symptoms, hypothyroidism may be overlooked in a patient with depression. The possibility of hypothyroidism should be considered especially noted in patients taking lithium carbonate. Furthermore, suspicion of hypothyroidism based on clinical findings during anesthesia may prompt the need for further evaluation.
• 血管内皮細胞におけるSARS-CoV-2侵入機構の解析(Analysis of SARS-CoV-2 uptake mechanism in vascular endothelial cells)
  桜井 優弥; 間石 奈湖; 藤岡 容一郎; 大場 雄介; 武田 遼; 佐々木 道仁; 大場 靖子; 樋田 泰浩; 澤 洋文; 樋田 京子
  日本病理学会会誌, 113, 1, 369, 369, (一社)日本病理学会, Feb. 2024
  English
• Viral uptake and pathophysiology of the lung endothelial cells in age-associated severe SARS-CoV-2 infection models.
  Takuya Tsumita; Ryo Takeda; Nako Maishi; Yasuhiro Hida; Michihito Sasaki; Yasuko Orba; Akihiko Sato; Shinsuke Toba; Wataru Ito; Takahito Teshirogi; Yuya Sakurai; Tomohiro Iba; Hisamichi Naito; Hitoshi Ando; Haruhisa Watanabe; Amane Mizuno; Toshiki Nakanishi; Aya Matsuda; Ren Zixiao; Ji-Won Lee; Tadahiro Iimura; Hirofumi Sawa; Kyoko Hida
  Aging cell, 23, 2, e14050, Feb. 2024, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Thrombosis is the major cause of death in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, and the pathology of vascular endothelial cells (ECs) has received much attention. Although there is evidence of the infection of ECs in human autopsy tissues, their detailed pathophysiology remains unclear due to the lack of animal model to study it. We used a mouse-adapted SARS-CoV-2 virus strain in young and mid-aged mice. Only mid-aged mice developed fatal pneumonia with thrombosis. Pulmonary ECs were isolated from these infected mice and RNA-Seq was performed. The pulmonary EC transcriptome revealed that significantly higher levels of viral genes were detected in ECs from mid-aged mice with upregulation of viral response genes such as DDX58 and IRF7. In addition, the thrombogenesis-related genes encoding PLAT, PF4, F3 PAI-1, and P-selectin were upregulated. In addition, the inflammation-related molecules such as CXCL2 and CXCL10 were upregulated in the mid-aged ECs upon viral infection. Our mouse model demonstrated that SARS-CoV-2 virus entry into aged vascular ECs upregulated thrombogenesis and inflammation-related genes and led to fatal pneumonia with thrombosis. Current results of EC transcriptome showed that EC uptake virus and become thrombogenic by activating neutrophils and platelets in the aged mice, suggesting age-associated EC response as a novel finding in human severe COVID-19.
• Suspected Accidental Infiltration of Rocuronium During General Anesthesia Induction: A Case Report.
  Yuya Sakurai; Makiko Shibuya; Ryuichi Okiji; Yuri Hase; Takayuki Hojo; Yukifumi Kimura; Toshiaki Fujisawa
  Anesthesia progress, 70, 3, 116, 119, 01 Sep. 2023, [Peer-reviewed], [Lead author], [International Magazine]
  English, Scientific journal, There are few reports on rocuronium infiltration under general anesthesia. We report a case of suspected accidental rocuronium infiltration during anesthesia induction. A 25-year-old woman with autism spectrum disorder, intellectual disability, and epilepsy was scheduled for the extraction of 4 impacted third molars under general anesthesia. After induction with sevoflurane, an intravenous (IV) line was established in the left cephalic vein. Rocuronium was administered; however, subcutaneous swelling at the IV site was observed immediately. Spontaneous ventilations were maintained until additional rocuronium was administered via a new IV line. After heat pack application, the swelling disappeared 60 minutes after infiltration, and no tissue damage was observed. A strategy was developed to continue neuromuscular monitoring until recovery occurred. Acceleromyography was used, and the train-of-4 ratios at 99, 130, and 140 minutes after infiltration were 0.79, 0.91, and 1.0, respectively. Sugammadex was administered to prevent neuromuscular blockade recurrence. The patient was extubated once adequate return of muscle function and consciousness were observed. No neuromuscular block prolongation or recurrence were observed postoperatively. When rocuronium infiltration is suspected, it is important to eliminate swelling at the infiltration site and determine a management strategy based on neuromuscular monitoring.
• Smith-Magenis症候群患者の歯科治療における全身麻酔管理経験
  置地 竜一; 詫間 滋; 桜井 優弥; 長谷 由理; 石田 義幸; 藤澤 俊明
  日本歯科麻酔学会雑誌, 51, 1, 10, 12, (一社)日本歯科麻酔学会, Jan. 2023, [Peer-reviewed]
  Japanese, 症例は6歳9ヵ月女児で、重度の睡眠障害、行動異常を伴うSmith-Magenis症候群患者の多数歯う蝕に対し、全身麻酔下に保存修復処置、根管治療、抜歯など全顎的歯科治療が予定された。意思疎通困難で未知の事象に対する恐怖心が強い傾向にあり、衝動的な摂食行動を抑制すると自傷他害が起こる可能性があったため、前日入院時に周囲に飲食物がない環境を整え、当日は本患児と他患者の朝食の配膳を調整することで異常行動なく手術室に入室できた。麻酔導入・覚醒時の興奮に備えて、導入時の恐怖心を減らすための配慮とプロポフォールでの麻酔維持を行った。その結果、抑制の必要なく緩徐導入が可能となり、麻酔維持中は特記事項なく推移し、覚醒後も行動異常や危険行動を認めなかった。本症候群患者の周術期管理に際しては、術前に行動異常の有無とその内容を十分把握した上で、対策を講じることが重要である。

• Analysis of the mechanism of enhanced vascular endothelial cell SARS-CoV-2 infection with aging
  SAKURAI Yuya; MAISHI Nako; FUJIOKA Yoichiro; OHBA Yusuke; SASAKI Michihito; OHBA Yasuko; HIDA Yasuhiro; SAWA Hirofumi; HIDA Kyoko, 日本病理学会会誌, 114, 1, 2025
• 凝固機能検査異常が軽度でも重篤な血液疾患が隠れている可能性がある
  木村幸文; 小野寺舞; 小野澤知也; 石原穂乃香; 多田早織; 桜井優弥; 手代木孝仁; 工藤葉子; 石川恵美; 北條敬之; 長谷由理; 新田幸絵; 渋谷真希子; 城戸幹太, 北海道臨床歯科麻酔学会プログラム・抄録集, 39th, 2025
• 加齢に伴う血管内皮細胞SARS-CoV-2感染増強メカニズムの解析
  桜井優弥; 桜井優弥; 間石奈湖; 藤岡容一朗; 大場雄介; 佐々木道仁; 佐々木道仁; 大場靖子; 大場靖子; 大場靖子; 樋田泰浩; 城戸幹太; 澤洋文; 澤洋文; 澤洋文; 澤洋文; 樋田京子, 日本歯科医師会雑誌, 78, 4, 2025
• Analysis of the mechanism of enhanced SARS-CoV-2 infection of vascular endothelial cell with aging
  桜井優弥; 桜井優弥; 間石奈湖; 松田彩; 武田遼; 伊藤航; 樋田京子, 日本臨床口腔病理学会総会・学術大会プログラム・抄録集(Web), 35th, 2024
• 血管内皮細胞におけるSARS-CoV-2侵入機構の解析
  桜井優弥; 間石奈湖; 藤岡容一朗; 大場雄介; 武田遼; 佐々木道仁; 大場靖子; 樋田泰浩; 澤洋文; 澤洋文; 樋田京子, 日本血管生物医学会学術集会プログラム・抄録集(CD-ROM), 31st, 2023
• 麻酔導入時にロクロニウムの血管外漏出を認めた一例
  桜井優弥; 渋谷真希子; 長谷由理; 北條敬之; 木村幸文; 藤澤俊明, 日本歯科麻酔学会雑誌(Web), 49, 2021

• 加齢に伴う血管内皮細胞SARS-CoV-2感染増強メカニズムの解析
  桜井優弥; 間石奈湖; 藤岡容一朗; 大場雄介; 佐々木道仁; 大場靖子; 樋田泰浩; 城戸幹太; 澤洋文; 樋田京子
  第25回 ⽇本⻭科医学会学術⼤会
  26 Sep. 2025
• 加齢に伴う血管内皮細胞SARS-CoV-2感染増強メカニズムの解析
  桜井優弥; 間石奈湖; 藤岡容一朗; 大場雄介; 佐々木道仁; 大場靖子; 樋田泰浩; 澤洋文; 樋田京子
  第114回日本病理学会総会, 19 Apr. 2025, Oral presentation
• 血管の加齢変化がもたらすSARS-CoV-2取り込み増加のメカニズム
  桜井優弥; 間石奈湖; 藤岡容一朗; 大場雄介; 武田遼; 佐々木道仁; 大場靖子; 樋田泰浩; 澤洋文; 樋田京子
  日本血管生物医学会特別集会, 22 Feb. 2025
• 加齢に伴う血管内皮細胞SARS-CoV-2感染増強メカニズムの解析
  桜井優弥; 間石奈湖; 藤岡容一朗; 大場雄介; 武田遼; 佐々木道仁; 大場靖子; 樋田泰浩; 澤洋文; 樋田京子
  第32回 日本血管生物医学会学術集会, 07 Dec. 2024
• 血管の加齢変化がもたらすSARS-CoV-2取り込みの増加メカニズム
  桜井優弥; 間石奈湖; 藤岡容一朗; 大場雄介; 武田遼; 佐々木道仁; 大場靖子; 樋田泰浩; 澤洋文; 樋田京子
  第10回 北大・部局横断シンポジウム, 06 Sep. 2024
• 血管の加齢変化がもたらすSARS-CoV-2取り込み増加のメカニズム
  桜井優弥; 間石奈湖; 藤岡容一朗; 大場雄介; 武田遼; 佐々木道仁; 大場靖子; 樋田泰浩; 澤洋文; 樋田京子
  第19回 先端歯学スクール(先端歯学2024), 23 Aug. 2024
• 血管の加齢変化がもたらすSARS-CoV-2取り込みの増加メカニズム
  桜井優弥; 間石奈湖; 藤岡容一朗; 大場雄介; 武田遼; 佐々木道仁; 大場靖子; 樋田泰浩; 澤洋文; 樋田京子
  第2回新型コロナウイルス研究集会, 04 Aug. 2024
• Analysis of the mechanism of enhanced SARS-CoV-2 infection of vascular endothelial cell with aging
  桜井優弥; 間石奈湖; 松田彩; 武田遼; 伊藤航; 樋田京子
  第35回 日本臨床口腔病理学会 総会・学術大会, 31 Jul. 2024
• 血管内皮細胞におけるSARS-CoV-2侵入機構の解析(Analysis of SARS-CoV-2 uptake mechanism in vascular endothelial cells)
  桜井 優弥; 間石 奈湖; 藤岡 容一郎; 大場 雄介; 武田 遼; 佐々木 道仁; 大場 靖子; 樋田 泰浩; 澤 洋文; 樋田 京子
  日本病理学会会誌, Feb. 2024, (一社)日本病理学会, English
  Feb. 2024 - Feb. 2024
• Analysis of SARS-CoV-2 uptake mechanism in vascular endothelial cells
  SAKURAI Yuya; MAISHI Nako; FUJIOKA Yoichiro; OHBA Yusuke; TAKEDA Ryo; SASAKI Michihito; OHBA Yasuko; HIDA Yasuhiro; SAWA Hirofumi; HIDA Kyoko
  日本病理学会会誌, 2024
  2024 - 2024
• 加齢に伴う新型コロナウイルス感染症重症化におけるSARS-CoV-2感染血管内皮細胞EVsの役割
  桜井 優弥; 間石 奈湖; 松田 彩; 吉岡 祐亮; 武田 遼; 積田 卓也; 伊藤 航; 手代木 孝仁; 佐々木 道仁; 大場 靖子; 佐藤 彰彦; 鳥羽 晋輔; 樋田 泰浩; 澤 洋文; 城戸 幹太; 落谷 孝広; 樋田 京子
  第10回 日本細胞外小胞学会学術集会, 23 Oct. 2023
• Analysis of SARS-CoV-2 uptake mechanism in vascular endothelial cells
  桜井優弥; 間石奈湖; 松田彩; 樋田京子
  第65回歯科基礎医学会学術大会, 16 Sep. 2023
• 血管内皮細胞におけるSARS-CoV-2侵入機構の解析
  桜井 優弥; 間石 奈湖; 松田 彩; 樋田 京子
  Journal of Oral Biosciences Supplement, Sep. 2023, (一社)歯科基礎医学会, Japanese
  Sep. 2023 - Sep. 2023
• Anesthetic Management for a Patient with Smith-Magenis Syndrome
  置地竜一; 置地竜一; 詫間滋; 桜井優弥; 長谷由理; 石田義幸; 藤澤俊明
  日本歯科麻酔学会雑誌(Web), 2023
  2023 - 2023
• 血管内皮細胞におけるSARS-CoV-2侵入機構の解析
  桜井優弥; 間石奈湖; 藤岡容一朗; 大場雄介; 武田遼; 佐々木道仁; 大場靖子; 樋田泰浩; 澤洋文; 澤洋文; 樋田京子
  日本血管生物医学会学術集会プログラム・抄録集, 2023
  2023 - 2023
• 麻酔導入時にロクロニウムの血管外漏出を認めた一例
  桜井 優弥; 渋谷 真希子; 長谷 由理; 北條 敬之; 木村 幸文; 藤澤 俊明
  日本歯科麻酔学会雑誌, Sep. 2021, (一社)日本歯科麻酔学会, Japanese
  Sep. 2021 - Sep. 2021

• 感染症によってリンパ管様形質転換を示す特異的血管内皮細胞の病態解析
  科学研究費助成事業
  01 Apr. 2026 - 31 Mar. 2029
  桜井 優弥
  日本学術振興会, 若手研究, 北海道大学, 26K18605
• 血管内皮細胞の細胞老化によるCOVID-19重症化メカニズムの解明
  科学研究費助成事業
  31 Jul. 2025 - 31 Mar. 2027
  桜井 優弥
  日本学術振興会, 研究活動スタート支援, 北海道大学, 25K23803
• 重症ウイルス感染症で出現するリンパ管マーカーLYVE1陽性血管内皮細胞の病態的役割の解析
  寿原記念財団研究助成
  Apr. 2026 - Mar. 2027
  公益財団法人 寿原記念財団, Principal investigator
• 血管内皮細胞の細胞老化によるCOVID-19重症化機構の解明
  若手研究人材育成事業 （若手研究人材・ネットワーク育成補助金（タレント補助金））
  Aug. 2025 - Mar. 2026
  公益財団法人北海道科学技術総合振興センター, Principal investigator
• ウイルス感染症と血管の加齢変化の病態的関係の解明
  令和7年度(2025年度) 一般財団法人 湯浅記念会 調査研究事業助成金
  一般財団法人 湯浅記念会