博士（歯学）, 東北大学

Life Science, Oral pathobiological science

Life Science, Oral biological science

Bachelor's degree program, School of Dental Medicine

Doctoral (PhD) degree program, Graduate school of Dental Medicine

2025, 日本矯正歯科学会, 第9回論文賞
永山哲聖;福永智広;矢島健太;茂見翔平;坂本麻由里;清流正弘;市川博之;溝口到;佐藤匡

Jun. 2018, 東北大学歯学会, 研究奨励賞
佐藤 匡

Sep. 2012, 歯科基礎医学会, 優秀ポスター賞
佐藤 匡

Mar. 2012, 東北大学 総長賞
佐藤 匡

Functional role of peripheral P2X3 receptor expression in trigeminal ganglion neurons: a microarray-based evaluation of the antagonist effect of P2X3.
Mika Ishikawa; Takehiro Yajima; Kentaro Mizuta; Tadasu Sato
Purinergic signalling, 22, 3, Apr. 2026, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, The P2X receptor is an extracellular adenosine triphosphate (ATP)-gated ion channel that plays critical roles in cellular and developmental processes, including cell proliferation, survival, and differentiation. Specifically, P2X3 is essential for ATP to function as a neurotransmitter in the peripheral nervous system. Despite the role of P2X3 in nociception, its specific contribution to the development of the trigeminal ganglion (TG), particularly across different developmental stages, remains undetermined. Therefore, in this study, we measured P2X3 expression in the mouse TG and peripheral tissues, investigated its function in the newborn mouse with P2X3 antagonist, and evaluated its effects using a microarray analysis. We investigated the size and number of P2X3-positive neurons in the TG and the density of P2X3-positive nerve fibers in peripheral tissues of newborn and adult mice using immunohistochemical techniques. P2X3-immunoreactivity occurred in 97.9% of the sensory neurons in the TG at P0 (postnatal day 0); however, the proportion of P2X3-positive TG neurons gradually decreased over time. By adulthood, only 45.0% of sensory neurons in the TG were immunoreactive for P2X3; these neurons were predominantly small- to medium-sized. In the vibrissa, the rate of P2X3-immunoreactive peripheral nerve fibers was significantly higher at P0 than at the adult stage, with a notable decrease during maturation. Notably, administration of a P2X3 antagonist to the vibrissal pad led to reduced leptin receptor transcription in TG neurons of neonatal mice. Collectively, these findings suggest that P2X3 plays a crucial role in the development and maturation of the TG and its associated peripheral regions.

Distribution of PGP 9.5- and nNOS-containing nerve fibers in the temporomandibular joint disk attachments of the elderly human.
Tadasu Sato; Hirotaka Ishigaki; Takehiro Yajima; Daisuke Tachiya; Hiroyuki Ichikawa
Tissue & cell, 99, 103282, 103282, Apr. 2026, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
English, Scientific journal, Innervation of the human temporomandibular joint (TMJ) disk and its surrounding structures posterior vascular plexus portion was investigated by immunohistochemistry for protein gene product 9.5 (PGP 9.5) and neuronal nitric oxide synthase (nNOS). The TMJ disk was free of PGP 9.5- and nNOS-immunoreactive (-ir) nerve fibers. In the TMJ disk attachments and posterior vascular plexus portion, however, PGP 9.5- and nNOS-immunoreactivity was detected. PGP 9.5-ir nerve fibers within nerve bundles were common in these regions. Perivascular PGP 9.5-ir nerve fibers were common in the attachments and posterior vascular plexus portion but not in the posterior attachment inferior. Isolated PGP 9.5-ir nerve fibers were also scattered throughout the TMJ attachments but not in the posterior vascular plexus portion. There was no significant difference about the density of PGP 9.5-ir nerve fibers among all of the attachments and posterior vascular plexus portion. NOS-ir nerve fibers within nerve bundles were common in the anterior and posterior attachments inferior, and the posterior vascular plexus portion. Perivascular nNOS-ir nerve fibers in the anterior attachment inferior were more abundant than in other regions. Isolated nNOS-ir nerve fibers were common in the anterior attachment inferior but not in other regions. Fine isolated nerve fibers with varicose appearance occasionally had small swellings in their terminals. Thick isolated nerve fibers rarely showed unencapsulated corpuscular endings with multiple large and spindle-shaped swellings. The density of nNOS-ir nerve fibers in the anterior attachment inferior was significantly higher than in the posterior attachment superior. In addition, the double immunofluorescence method demonstrated that vascular and isolated nNOS-ir nerve fibers were also immunoreactive for PGP 9.5 in the anterior attachment inferior. The present study suggests that nNOS-ir nerve fibers with a parasympathetic nature can control blood flow mainly in the anterior attachment inferior and slightly in other attachments and the posterior vascular plexus portion. nNOS-ir nerve fibers with a sensory nature may be associated with nociceptive transmission in the TMJ disk attachments.

Distribution and possible function of transient receptor potential melastatin-7 in the rat cranial sensory ganglia
Hiroka Tsumaki; Tomohiro Fukunaga; Kaori Takahashi; Takehiro Yajima; Kenji Imamachi; Yuji Hatakeyama; Hiroyasu Kanetaka; Shigeru Takahashi; Tadasu Sato
Biomedical research (Tokyo, Japan), 46, 5, 215, 224, Sep. 2025, [Peer-reviewed], [Last author, Corresponding author]
English, Scientific journal

迷走神経上神経節ならびに下神経節における温度センサーの分布
佐藤 匡; 今待賢治; 矢島健大; 髙橋 茂
北海道歯学雑誌, 46, 14, 17, Sep. 2025, [Invited], [Lead author, Corresponding author]

Apoptotic cell death during regressive changes in salivary glands: A morphological perspective
Shigeru Takahashi; Akihiro Nezu; Akihiko Tanimura; Chikage Tamura; Kenji Imamachi; Tadasu Sato
Journal of Oral Biosciences, 67, 1, 100585, Elsevier BV, Mar. 2025, [Peer-reviewed], [Invited], [Last author]
Scientific journal

Distribution of alpha‐synuclein in rat salivary glands
Tomiko Nishitani; Yoshihiro Mitoh; Takehiro Yajima; Daisuke Tachiya; Tomohiro Hoshika; Tomohiro Fukunaga; Yoshihiro Nishitani; Ryusuke Yoshida; Itaru Mizoguchi; Hiroyuki Ichikawa; Tadasu Sato
The Anatomical Record, 307, 8, 2933, 2946, Wiley, Aug. 2024, [Peer-reviewed], [Last author, Corresponding author]
Scientific journal, Abstract

Expression of alpha‐synuclein (Syn), a presynaptic neuronal protein, was immunohistochemically examined in intact rat submandibular, sublingual, and lingual glands. The submandibular gland contained abundant periductal Syn‐immunoreactive (−ir) nerve fibers. Abundant Syn‐ir varicosities were present in acini of the sublingual and serous lingual glands. By confocal laser scanning microscopy, Syn‐ir nerve fibers around smooth muscle actin (SMA)‐ir cells alone were infrequent; however, those around aquaporin‐5 (AQP5)‐ir cells alone and both SMA‐ and AQP5‐ir cells were abundant in the sublingual and serous lingual glands. SMA‐ir cells were occasionally immunoreactive for toll‐like receptor 4, a Syn receptor. Syn‐ir nerve fibers contained tyrosine hydroxylase (TH) in the submandibular gland and choline acetyltransferase (ChAT) in all examined salivary glands. In the superior cervical (SCG), submandibular, and intralingual ganglia, sympathetic and parasympathetic neurons co‐expressed Syn with TH and ChAT, respectively. SCG neurons innervating the submandibular gland contained mostly Syn. In the thoracic spinal cord, 14.7% of ChAT‐ir preganglionic sympathetic neurons co‐expressed Syn. In the superior salivatory nucleus, preganglionic parasympathetic neurons projecting to the lingual nerve co‐expressed Syn and ChAT. The present findings indicate that released Syn acts on myoepithelial cells. Syn in pre‐ and post‐ganglionic neurons may regulate neurotransmitter release and salivary volume and composition.

Neural sodium voltage-gated channel beta subunit 3 and alpha subunit 7 distributions in mouse periodontal ligaments after experimental tooth movement
Tessei Nagayama; Tomohiro Fukunaga; Takehiro Yajima; Shohei Shigemi; Mayuri Sakamoto; Masahiro Seiryu; Hiroyuki Ichikawa; Itaru Mizoguchi; Tadasu Sato
Clinical and Investigative Orthodontics, 83, 2, 70, 79, Informa UK Limited, 28 Mar. 2024, [Peer-reviewed], [Last author, Corresponding author]
Scientific journal

Distribution and anti-nociceptive function of endomorphin-1 in the rat cranial sensory ganglia.
Shun Sato; Tadasu Sato; Takehiro Yajima; Daisuke Tachiya; Tetsu Takahashi; Shinnosuke Nogami; Masahiro Saito; Kensuke Yamauchi; Hiroyuki Ichikawa
Biomedical research (Tokyo, Japan), 45, 2, 77, 89, Mar. 2024, [Peer-reviewed], [Corresponding author], [Domestic magazines]
English, Scientific journal, Distribution of endomorphin-1 (EM-1) was immunohistochemically investigated in the rat cranial sensory ganglia. Small to medium-sized neurons in the trigeminal (TG), petrosal (PG), and jugular ganglia (JG) expressed EM-1-immunoreactivity. However, EM-1-immunoreactive (-ir) neurons were infrequent in the nodose ganglion. In the brainstem, EM-1-ir varicose fibers were detected in the superficial layer of the medullary dorsal horn and the caudal part of the nucleus tractus solitarius. By trichrome immunofluorescence analysis, approximately 70% of EM-1-ir neurons were also immunoreactive for transient receptor potential vanilloid 1 (TRPV1) in all the examined ganglia. Additionally, 56.8% of EM1-ir TG neurons and approximately 30% of EM-1-ir PG and JG neurons showed calcitonin gene-related peptide (CGRP)-immunoreactivity. By a retrograde tracing method, several TG, PG, and JG neurons innervating the facial and external ear canal skin expressed EM-1-immunoreactivity. However, EM-1-ir neurons innervating the tooth pulp, circumvallate papilla, and pharynx were relatively rare. Thus, EM-1 expression and its coexistence with TRPV1 and CGRP in the cranial sensory neurons may depend on their various peripheral targets. EM1-ir neurons probably project to the superficial layer of the medullary dorsal horn and caudal part of the nucleus tractus solitarius. EM-1 may be involved in nociceptive transmission from the skin.

The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats
Shohei Shigemi; Tadasu Sato; Mayuri Sakamoto; Takehiro Yajima; Takahiro Honda; Hiroka Tsumaki; Toru Deguchi; Hiroyuki Ichikawa; Tomohiro Fukunaga; Itaru Mizoguchi
Scientific Reports, 13, 1, Springer Science and Business Media LLC, 22 Aug. 2023, [Peer-reviewed]
Scientific journal, Abstract

When orthodontic forces are applied to teeth, bone remodeling, which consists of bone resorption and bone formation, occurs around the teeth. Transient receptor potential vanilloid 2 (TRPV2) is a cation channel expressed in various cell types that responds to various stimuli, including mechanical stress, and involved in calcium oscillations during the early stages of osteoclast differentiation. However, in vivo expression of TRPV2 in osteoclasts has not yet been reported, and temporo-spatial expression of TRPV2 during osteoclast differentiation is unclear. In this study, we examined the TRPV2 expression during experimental tooth movement and assessed the effect of TRPV2 on osteoclast differentiation. TRPV2 was detected on day 1 after experimental tooth movement on the compression side, and the number of TRPV2-expressing cells significantly increased on day 7. These TRPV2-expressing cells had a single, or multiple nuclei and were positive for TRAP activity. Consistent with these in vivo findings, in vitro experiments using RAW264.7 osteoclast progenitor cells showed that TRPV2 mRNA was increased at the early stage of osteoclast differentiation and maintained until the late stage. Furthermore, a TRPV2 channel selective antagonist significantly inhibited osteoclast differentiation. These findings suggest that TRPV2 may have a regulatory role in osteoclast differentiation during orthodontic tooth movement.

Transient receptor potential melastatin-7 in the rat dorsal root ganglion.
Takehiro Yajima; Tadasu Sato; Hiroshi Hosokawa; Teruyoshi Kondo; Hiroyuki Ichikawa
Journal of chemical neuroanatomy, 102163, 16 Sep. 2022, [Peer-reviewed], [Corresponding author], [International Magazine]
English, Scientific journal, AIMS: Transient receptor potential melastatin-7 (TRPM7) is a selective cation permeable channel which plays important roles in cellular and developmental biology such as cell proliferation, survival, differentiation and migration. This channel is also known to be necessary for transmitter release in the peripheral nervous system. In this study, immunohistochemistry for TRPM7 was conducted in the rat lumbar dorsal root ganglion (DRG). METHODS: Triple immunofluorescence methods were used to demonstrate distribution of TRPM7 and its relationship to other TRP channels in the DRG. Retrograde tracing and double immunofluorescence methods were also performed to know peripheral targets of DRG neurons containing TRPM7 and TRP vanilloid 1 (TRPV1). In addition, transection of the sciatic nerve was conducted to demonstrate an effect of the nerve injury on TRPM7expression in the DRG. RESULTS: TRPM7-immunoreactivity was expressed by 53.9% of sensory neurons in the 4th lumbar DRG. TRPM7-immunoreactive (-IR) DRG neurons mostly had small (<600μm²) and medium-sized (600-1200μm²) cell bodies. By triple and double immunofluorescence methods, approximately 70% of TRPM7-IR DRG neurons contained TRPV1-immunoreactivity. Although the number of DRG neurons co-expressing TRPM7 and TRPM8 was small in the DRG, almost all of TRPM8-IR DRG neurons co-expressed TRPM7-immunoreactivity. By combination of retrograde tracing method and immunohistochemistry, TRPM7 was expressed by half of DRG neurons innervating the plantar skin (61.9%) and gastrocnemius muscle (51.2%), and 79.6% of DRG neurons innervating the periosteum. Co-expression of TRPM7 and TRPV1 among periosteum DRG neurons (75.7%) was more abundant than among cutaneous (53.2%) and muscular (40.4%) DRG neurons. DRG neurons which co-expressed these ion channels in the periosteum had smaller cell bodies compared to the skin and muscle. In addition, the sciatic nerve transection decreased the number of TRPM7-IR neurons in the DRG (approximately 60% reduction). The RT-qPCR analysis also demonstrated reduction of TRPM7 mRNA in the injured DRG. CONCLUSION: The present study suggests that TRPM7 is mainly located in small nociceptors in the DRG. The content of TRPM7 in DRG neurons is probably different among their peripheral targets. TRPM7 in DRG neurons may be able to respond to noxious stimulation from their peripheral tissues. The nerve injury can decrease the level of TRPM7 mRNA and protein in DRG neurons.

Innervation of the human minor salivary glands; immunohistochemical study for neurochemical substances.
Ayumi Sato; Takehiro Yajima; Daisuke Tachiya; Hiroyuki Ichikawa; Tadasu Sato
Tissue & cell, 79, 101934, 14 Sep. 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Distributions of choline acetyltransferase (ChAT), vasoactive intestinal polypeptide (VIP), dopamine β-hydroxylase (DBH), tyrosine hydroxylase (TH), neuropeptide Y (NPY) and the transient receptor potential cation channel subfamily V member 2 (TRPV2) were examined in the human minor salivary glands. ChAT-, VIP- and DBH-immunoreactive (-IR) nerve fibers were detected within nerve bundles and close to blood vessels and ducts in the salivary glands. Periacinar nerve fibers were commonly immunoreactive for ChAT in the Ebner's gland but infrequently in other salivary glands. Periacinar VIP-IR nerve fibers were numerous in the palatal gland, moderate in the lingual gland and relatively rare in the labial and Ebner's glands. Some TH-, NPY- and TRPV2-IR nerve fibers were also present around blood vessels and glandular acini in the palatal, lingual and Ebner's glands. Neuronal cells in the vicinity of Ebner's and lingual glands were immunoreactive for ChAT, VIP, TH and TRPV2. By confocal laser scanning microscopy, VIP- and ChAT-IR varicosities were located in the vicinity of myoepithelial and acinar cells in the minor salivary glands. The human minor salivary glands are probably innervated by parasympathetic and sympathetic nerves. Neurotransmitters including neuropeptides in these nerves are thought to be correlated to vasodilation and/or salivary secretion. Acetylcholine and VIP may regulate secretion of the saliva and its components in the salivary glands.

Distribution of neuronal cells which contain dopamine β-hydroxylase, tyrosine hydroxylase, neuropeptide Y and vasoactive intestinal polypeptide in the human internal carotid nerve.
Tadasu Sato; Takehiro Yajima; Souichi Kokubun; Daisuke Tachiya; Hiroyuki Ichikawa
Anatomical record (Hoboken, N.J. : 2007), 305, 5, 1277, 1286, May 2022, [Peer-reviewed], [Lead author], [International Magazine]
English, Scientific journal, The human internal carotid nerve (ICN) occasionally has a swelling beneath the external opening of the carotid canal. In this study, the presence and distribution of neuronal cells were investigated in the bilateral ICNs of nine human cadavers. Among 44.4% of the cadavers, swellings were detected in the ICN. Their diameters ranged from 1.7 to 3.6 mm (average ± SD = 2.6 ± 0.7 mm). Thirty-eight percent of these swellings were large (diameter > 3 mm) and showed an oval shape. The large swelling contained many neuronal cells. However, the ICNs with or without a swelling <3 mm diameter were mostly free from neuronal cells (93.3%). Only in one human cadaver, the right ICN without a swelling had a small number of neuronal cells. By the present immunohistochemical method, ICN neurons contained catecholamine-synthesizing enzymes and neuropeptides. Dopamine-beta hydroxylase- and tyrosine hydroxylase-immunoreactivity were mostly expressed by ICN neurons. More than half of them also contained neuropeptide Y-immunoreactivity. However, vasoactive intestinal polypeptide-immunoreactive ICN neurons were relatively infrequent. Substance P- and calcitonin gene-related peptide-immunoreactive ICN neurons could not be detected. By the cell size analysis, neuropeptide Y-immunoreactive neurons were significantly smaller than neuropeptide Y-immunonegative neurons in the ICN. The present study suggests that ICN neurons have a sympathetic function in the human.

Distribution and possible function of galanin about headache and immune system in the rat dura mater.
Kenichiro Shimazaki; Takehiro Yajima; Hiroyuki Ichikawa; Tadasu Sato
Scientific reports, 12, 1, 5206, 25 Mar. 2022, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, Galanin (GAL) is a nociceptive transmitter or modulator in the trigeminal sensory system. In this study, GAL expression was investigated in the rat dura mater to demonstrate its possible function in headache using immunohistochemical techniques. The cerebral falx and cerebellar dura mater received abundant blood and nerve supply, and were significantly thicker compared to other portions in the cerebral dura mater. GAL-immunoreactivity was expressed by cell and nerve fiber profiles. Presumed macrophages and dendritic cells contained GAL-immunoreactivity, and co-expressed with CD11b-immunoreactivity. Many isolated and perivascular nerve fibers also showed GAL-immunoreactivity. In addition, GAL-immunoreactive nerve fibers were present in the vicinity of macrophages and dendritic cells with either GAL- or ED1-immunoreactivity. GAL-immunoreactive cells and nerve fibers were common in the cerebral falx and cerebellar dura mater and infrequent in other portions. And, GAL-immunoreactive nerve fibers usually co-expressed calcitonin gene-related peptide (CGRP)-immunoreactivity. In the trigeminal ganglion, a substantial proportion of sensory neurons innervating the dura mater contained GAL-immunoreactivity (mean ± SD, 3.4 ± 2.2%), and co-expressed CGRP-immunoreactivity (2.7 ± 2.1%). The present study may suggest that GAL is associated with nociceptive transduction or modulation in the dura mater. GAL also possibly plays a role in the immune mechanism of the dura mater.

Distribution of alpha-synuclein in the rat cranial sensory ganglia, and oro-cervical regions.
Tadasu Sato; Takehiro Yajima; Shiori Saijyo; Kenichiro Shimazaki; Tomiko Nishitani; Tomohiro Hoshika; Yoshihiro Nishitani; Hiroyuki Ichikawa; Itaru Mizoguchi; Tomohiro Fukunaga
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft, 238, 151776, Nov. 2021, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
English, Scientific journal, BACKGROUND: Alpha-synuclein (Syn), an unfolded soluble cytosolic protein, is known as a disease-associated protein in the brain. However, little is known about distribution of this protein in the peripheral nervous system. In this study, expression of Syn was investigated in the sensory ganglia of the cranial nerves V, IX and X. METHODS: To analyze distribution of Syn and its co-expression with calcitonin gene-related peptide (CGRP) or the transient receptor potential cation channel subfamily V member 1 (TRPV1), immunohistochemical techniques were used in the rat cranial sensory ganglia and their peripheral tissues. RESULTS: Syn-immunoreactive (-ir) neurons were abundant in the sensory ganglia of the petrosal (56.7%), jugular (28.3%) and nodose ganglia (82.5%). These neurons had small to medium-sized cell bodies (petrosal, mean ± S.D. = 667.4 ± 310.8 μ m2; jugular, 625.1 ± 318.4 μ m2; nodose, 708.3 ± 248.3 μ m2), and were distributed throughout the ganglia. However, the trigeminal ganglion was mostly free of Syn-ir neurons. By double and triple immunofluorescence staining, Syn-ir neurons co-expressed CGRP and TRPV1 in the petrosal and jugular ganglia. Syn-immunoreactivity was expressed by nerve fibers in the epithelium and taste bud of oral and cervical viscerae. These nerve fibers were abundant in the naso-pharynx, epiglottis and laryngeal vestibule. Some taste bud cells were also immunoreactive for Syn. In addition, Syn-ir nerve fibers were detected in the vicinity of macrophages, dendritic cells and Langerhans cells. CONCLUSIONS: Syn was abundant in the visceral sensory neurons but not in somatic sensory neurons. This protein may play a role in nociceptive and chemosensory transduction in the glossopharyngeal and vagal sensory ganglia. It is possible that Syn has a function about the immune mechanism of the upper air way.

Parasympathetic neurons in the human submandibular ganglion.
Mutsuko Kawashima; Takehiro Yajima; Daisuke Tachiya; Souichi Kokubun; Hiroyuki Ichikawa; Tadasu Sato
Tissue & cell, 70, 101496, 20 Jan. 2021, [Peer-reviewed], [Last author, Corresponding author], [International Magazine]
English, Scientific journal, The submandibular ganglion (SMG) contains parasympathetic neurons which innervate the submandibular gland. In this study, immunohistochemistry for vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), choline acetyltransferase (ChAT), dopamine β-hydroxylase (DBH), tyrosine hydroxylase (TH), and the transient receptor potential cation channel subfamily V members 1 (TRPV1) and 2 (TRPV2) was performed on the human SMG. In the SMG, 17.5 % and 8.9 % of parasympathetic neurons were immunoreactive for VIP and TRPV2, respectively. SMG neurons mostly contained ChAT- and DBH-immunoreactivity. In addition, subpopulations of SMG neurons were surrounded by VIP (69.6 %)-, TRPV2 (54.4 %)- and DBH (9.5 %)-immunoreactive (-ir) nerve fibers. SMG neurons with pericellular VIP- and TRPV2-ir nerve fibers were significantly larger than VIP- and TRPV2-ir SMG neurons, respectively. Other neurochemical substances were rare in the SMG. In the human submandibular gland, TRPV1- and TRPV2-ir nerve fiber profiles were seen around blood vessels. Double fluorescence method also demonstrated that TRPV2-ir nerve fiber profiles were located around myoepithelial and acinar cells in the submandibular gland. VIP and TRPV2 are probably expressed by both pre- and post-ganglionic neurons innervating the submandibular and sublingual glands. VIP, DBH and TRPV2 may have functions about regulation of salivary components in the salivary glands and neuronal activity in the SMG.

Orexin A and B in the rat superior salivatory nucleus
Tadasu Sato; Takehiro Yajima; Masako Fujita; Motoi Kobashi; Hiroyuki Ichikawa; Ryusuke Yoshida; Yoshihiro Mitoh
Autonomic Neuroscience: Basic and Clinical, 228, Nov. 2020, [Peer-reviewed], [Lead author]
Scientific journal

Effects of trigeminal nerve injury on the expression of galanin and its receptors in the rat trigeminal ganglion.
Fei Liu; Takehiro Yajima; Min Wang; Jie-Fei Shen; Hiroyuki Ichikawa; Tadasu Sato
Neuropeptides, 84, 102098, 10 Oct. 2020, [Peer-reviewed], [Last author], [International Magazine]
English, Scientific journal, In the spinal nervous system, the expression of galanin (GAL) and galanin receptors (GALRs) that play important roles in the transmission and modulation of nociceptive information can be affected by nerve injury. However, in the trigeminal nervous system, the effects of trigeminal nerve injury on the expression of GAL are controversy in the previous studies. Besides, little is known about the effects of trigeminal nerve injury on the expression of GALRs. In the present study, the effects of trigeminal nerve injury on the expression of GAL and GALRs in the rat trigeminal ganglion (TG) were investigated by using quantitative real-time reverse transcription-polymerase chain reaction and immunohistochemistry. To identify the nerve-injured and nerve-uninjured TG neurons, activating transcription factor 3 (ATF3, the nerve-injured neuron marker) was stained by immunofluorescence. The levels of GAL mRNA in the rostral half and caudal half of the TG dramatically increased after transection of infraorbital nerve (ION) and inferior alveolar nerve (IAN), respectively. Immunohistochemical labeling of GAL and ATF3 revealed that GAL level was elevated in both injured and adjacent uninjured small and medium-sized TG neurons after ION/IAN transection. In addition, the levels of GAL2R-like immunoreactivity were reduced in both injured and adjacent uninjured TG neurons after ION/IAN transection, while levels of GAL1R and GAL3R-like immunoreactivity remained unchanged. Furthermore, the number of small to medium-sized TG neurons co-expressing GAL- and GAL1R/GAL2R/GAL3R-like immunoreactivity was significantly increased after ION/IAN transection. In line with previous studies in other spinal neuron systems, these results suggest that GAL and GALRs play functional roles in orofacial neuropathic pain and trigeminal nerve regeneration after trigeminal nerve injury.

Sensory neurons in the human jugular ganglion
Keiichiro Atsumi; Takehiro Yajima; Daisuke Tachiya; Souichi Kokubun; Noriaki Shoji; Takashi Sasano; Hiroyuki Ichikawa; Tadasu Sato
Tissue and Cell, 64, 101344, Jun. 2020, [Peer-reviewed], [Last author, Corresponding author]
Scientific journal

Distribution of postganglionic neurons which contain dopamine β-hydroxylase, tyrosine hydroxylase, neuropeptide Y and vasoactive intestinal polypeptide in the human middle cervical ganglion
Souichi Kokubun; Tadasu Sato; Takehiro Yajima; Hiroyuki Ichikawa
Tissue and Cell, 58, 42, 50, Jun. 2019, [Peer-reviewed], [Corresponding author]
Scientific journal

Transient receptor potential melastatin-3 in the rat sensory ganglia of the trigeminal, glossopharyngeal and vagus nerves
Takehiro Yajima; Tadasu Sato; Kenichiro Shimazaki; Hiroyuki Ichikawa
Journal of Chemical Neuroanatomy, 96, 116, 125, Mar. 2019, [Peer-reviewed]
Scientific journal

The transient receptor potential cation channel subfamily V members 1 and 2, P2X purinoceptor 3 and calcitonin gene-related peptide in sensory neurons of the rat trigeminal ganglion, innervating the periosteum, masseter muscle and facial skin.
Sato M; Sato T; Yajima T; Shimazaki K; Ichikawa H
Archives of oral biology, 96, 66, 73, Aug. 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, OBJECTIVE: Distribution of the transient receptor potential cation channel subfamily V members 1 (TRPV1) and 2 (TRPV2), and P2X purinoceptor 3 (P2 × 3) was investigated in rat trigeminal ganglion neurons innervating the periosteum, masseter muscle and facial skin. DESIGN: Double immunofluorescence method for TRPV1 and TRPV2 ion channels or ATP receptor P2 × 3 with calcitonin gene-related peptide (CGRP) was performed on trigeminal ganglion neurons retrogradely labeled from the mandibular periosteum, masseter muscle, or facial skin in 15 male Wistar rats. RESULTS: The cell size of periosteum neurons (mean ± S.D. = 810.7 ± 36.1 μ m2) was smaller than that of masseter muscle neurons (927.0 ± 75.6 μ m2), and larger than that of facial skin neurons (661.3 ± 82.2 μ m2). Periosteum neurons contained TRPV1- (26.7%), TRPV2- (47.1%) and P2 × 3-immunoreactivity (50.1%). Expression of TRPV2-immunoreactivity was more abundant among periosteum neurons than among facial skin neurons (16.1%). Regarding to TRPV1 and P2 × 3 expression, however, there was no significant difference between periosteum neurons and, masseter muscle and facial skin neurons. TRPV1- immunoreactive trigeminal ganglion neurons which innervated the periosteum, masseter muscle and facial skin mostly had small and medium-sized cell bodies, whereas TRPV2- and P2 × 3-immunoreactive trigeminal ganglion neurons innervating those tissues were of various cell body sizes. Approximately 20% of periosteum (19.2%), masseter muscle (19.2%) and facial skin (21.5%) neurons contained both TRPV1- and CGRP-immunoreactivity. Some periosteum neurons also co-expressed CGRP-immunoreactivity with TRPV2- (10.9%) or P2 × 3- immunoreactivity (11.1%). Distributions of perivascular and free nerve fibers containing CGRP and either TRPV1, TRPV2, or P2 × 3 were occasionally very similar in the mandibular periosteum. CONCLUSIONS: The present study indicated that trigeminal ganglion nociceptors innervating the periosteum as well as those innervating the masseter muscle and facial skin have vanilloid, acidic, thermal, mechanical and ATP sensors. In some periosteum neurons, CGRP may act as inflammatory mediator through activation of TRPV1, TRPV2 and P2 × 3.

Nerve Injury Increases the Expression of Alpha-2/Delta-1 Subunit of L-Type Calcium Channel in Sensory Neurons of Rat Spinal and Trigeminal Nerves
Daisuke Tachiya; Tadasu Sato; Hiroyuki Ichikawa
Annals of Neurosciences, 24, 4, 191, 200, S. Karger AG, 01 May 2018, [Peer-reviewed]
English, Scientific journal

Innervation of the Human Incisive Papilla: Comparison with Other Oral Regions.
Chiaki Endo; Tadasu Sato; Takehiro Yajima; Kaoru Igarashi; Hiroyuki Ichikawa
Cells, tissues, organs, 205, 2, 93, 104, 2018, [Peer-reviewed], [International Magazine]
English, Scientific journal, Immunohistochemistry for several neurochemical substances was performed on the human incisive papilla and other oral structures. Sodium channel alpha subunit 7 (SCN7A) protein-immunoreactive (IR) Schwann cells and protein gene product 9.5 (PGP 9.5)-IR nerve fibers made nerve plexuses beneath the epithelium of the palate, including the incisive papilla, tongue, and lip. SCN7A immunoreactivity could also be detected in lamellated and nonlamellated capsules of corpuscle endings. Lamellated SCN7A-IR corpuscle endings were mostly restricted to the mucous and cutaneous lips. These endings had thick and spiral-shaped PGP 9.5-IR axons without ramification. Nonlamellated SCN7A-IR corpuscle endings were most numerous in the incisive papilla among the oral regions. On the basis of axonal morphology, the nonlamellated endings were divided into simple and complex types. PGP 9.5-IR terminal axons in the simple type ran straight or meandered with slight ramification, whereas those in the complex type were densely entangled with abundant ramification. Substance P (SP)-, calcitonin gene-related peptide (CGRP)-, and transient receptor potential cation channel subfamily V member 2 (TRPV2)-IR varicose fibers were rarely seen beneath the epithelium of oral structures. The present study indicates that the human incisive papilla has many low-threshold mechanoreceptors with nonlamellated capsules. SP-, CGRP-, and TRPV2-containing nociceptors may be infrequent in the incisive papilla and other oral regions.

Distribution of CGRP and TRPV2 in Human Paranasal Sinuses
Tadasu Sato; Nobuyuki Sasahara; Noriyuki Kanda; Yu Sasaki; Yu Yamaguma; Souichi Kokubun; Takehiro Yajima; Hiroyuki Ichikawa
CELLS TISSUES ORGANS, 203, 1, 55, 64, 2017, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Sensory Neurons in the Human Geniculate Ganglion
Tadasu Sato; Yu Yamaguma; Yu Sasaki; Noriyuki Kanda; Nobuyuki Sasahara; Souichi Kokubun; Takehiro Yajima; Hiroyuki Ichikawa
CELLS TISSUES ORGANS, 204, 1, 49, 58, 2017, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

TRPC1, TRPC3, and TRPC4 in Rat Orofacial Structures
Masatoshi Fujita; Tadasu Sato; Takehiro Yajima; Eiji Masaki; Hiroyuki Ichikawa
CELLS TISSUES ORGANS, 204, 5-6, 293, 303, 2017, [Peer-reviewed]
English, Scientific journal

Dentin Bond Strength of the Universal Adhesives using the Colloidal Platinum Nanoparticles
KATSUMATA Aiichiro; KATSUMATA Tamaki; HOSHIKA Shuhei; HOSHIKA Tomohiro; NISHITANI Tomiko; YAJIMA Takehiro; SATO Tadasu; ICHIKAWA Hiroyuki; SANO Hidehiko; NISHITANI Yoshihiro
Journal of Oral Tissue Engineering, 15, 1, 2017

SCN2B in the Rat Trigeminal Ganglion and Trigeminal Sensory Nuclei
Yusuke Shimada; Tadasu Sato; Takehiro Yajima; Masatoshi Fujita; Naoya Hashimoto; Noriaki Shoji; Takashi Sasano; Hiroyuki Ichikawa
CELLULAR AND MOLECULAR NEUROBIOLOGY, 36, 8, 1399, 1408, Nov. 2016, [Peer-reviewed]
English, Scientific journal

Recent advances in basic research on the trigeminal ganglion
Tetsuya Goto; Seog Bae Oh; Mamoru Takeda; Masamichi Shinoda; Tadasu Sato; Kaori K. Gunjikake; Koichi Iwata
JOURNAL OF PHYSIOLOGICAL SCIENCES, 66, 5, 381, 386, Sep. 2016, [Peer-reviewed]
English, Scientific journal

SPARCL1-containing neurons in the human brainstem and sensory ganglion
Naoya Hashimoto; Tadasu Sato; Takehiro Yajima; Masatoshi Fujita; Ayumi Sato; Yoshinaka Shimizu; Yusuke Shimada; Noriaki Shoji; Takashi Sasano; Hiroyuki Ichikawa
SOMATOSENSORY AND MOTOR RESEARCH, 33, 2, 112, 117, Jun. 2016, [Peer-reviewed]
English, Scientific journal

Distribution of TRPV1 and TRPV2 in the human stellate ganglion and spinal cord
Souichi Kokubun; Tadasu Sato; Chikara Ogawa; Kai Kudo; Koju Goto; Yuki Fujii; Yoshinaka Shimizu; Hiroyuki Ichikawa
NEUROSCIENCE LETTERS, 590, 6, 11, Mar. 2015, [Peer-reviewed]
English, Scientific journal

Glial Reaction in the Spinal Cord of the Degenerating Muscle Mouse (Scn8a (dmu) )
Tadasu Sato; Masatoshi Fujita; Yoshinaka Shimizu; Hiroyasu Kanetaka; Leona W. G. Chu; Patrice D. Cote; Hiroyuki Ichikawa
NEUROCHEMICAL RESEARCH, 40, 1, 124, 129, Jan. 2015, [Peer-reviewed], [Lead author]
English, Scientific journal

Distribution of transient receptor potential melastatin-8-containing nerve fibers in rat oral and craniofacial structures
Takehiro Yajima; Tadasu Sato; Hiroshi Hosokawa; Teruyoshi Kondo; Masahiro Saito; Hidetoshi Shimauchi; Hiroyuki Ichikaw
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER, 201, 1, 5, 2015, [Peer-reviewed], [Corresponding author]
English, Scientific journal

Distribution of TRPVs, P2X3, and Parvalbumin in the Human Nodose Ganglion
Daisuke Sato; Tadasu Sato; Yusuke Urata; Takayuki Okajima; Shota Kawamura; Manatsu Kurita; Kenta Takahashi; Masakazu Nanno; Asami Watahiki; Souichi Kokubun; Yoshinaka Shimizu; Eriko Kasahara; Noriaki Shoji; Takashi Sasano; Hiroyuki Ichikawa
CELLULAR AND MOLECULAR NEUROBIOLOGY, 34, 6, 851, 858, Aug. 2014, [Peer-reviewed]
English, Scientific journal

Transgenic expression of neuronal dystonin isoform 2 partially rescues the disease phenotype of the dystonia musculorum mouse model of hereditary sensory autonomic neuropathy VI
Andrew Ferrier; Tadasu Sato; Yves De Repentigny; Sabrina Gibeault; Kunal Bhanot; Ryan W. O'Meara; Anisha Lynch-Godrei; Samantha F. Kornfeld; Kevin G. Young; Rashmi Kothary
HUMAN MOLECULAR GENETICS, 23, 10, 2694, 2710, May 2014, [Peer-reviewed]
English, Scientific journal

Effect of adenosine A1 receptor agonist on the enhanced excitability of spinal dorsal horn neurons after peripheral nerve injury
Daisuke Yamaguchi; Ryuji Terayama; Shinji Omura; Hiroki Tsuchiya; Tadasu Sato; Hiroyuki Ichikawa; Tomosada Sugimoto
INTERNATIONAL JOURNAL OF NEUROSCIENCE, 124, 3, 213, 222, Mar. 2014, [Peer-reviewed]
English, Scientific journal

Prednisolone Induces Microglial Activation in the Subnucleus Caudalis of the Rat Trigeminal Sensory Complex
Yu Endo; Noriaki Shoji; Yusuke Shimada; Eriko Kasahara; Masahiro Iikubo; Tadasu Sato; Takashi Sasano; Hiroyuki Ichikawa
CELLULAR AND MOLECULAR NEUROBIOLOGY, 34, 1, 95, 100, Jan. 2014, [Peer-reviewed]
English, Scientific journal

Up-regulation of brain-derived neurotrophic factor in the dorsal root ganglion of the rat bone cancer pain model
Naoto Tomotsuka; Ryuji Kaku; Norihiko Obata; Yoshikazu Matsuoka; Hirotaka Kanzaki; Arata Taniguchi; Noriko Muto; Hiroki Omiya; Yoshitaro Itano; Tadasu Sato; Hiroyuki Ichikawa; Satoshi Mizobuchi; Hiroshi Morimatsu
JOURNAL OF PAIN RESEARCH, 7, 415, 423, 2014, [Peer-reviewed]
English, Scientific journal

The distribution of galanin-immunoreactive nerve fibers in the rat pharynx
Toshihiko Suzuki; Tadasu Sato; Mitsuhiro Kano; Hiroyuki Ichikawa
Neuropeptides, 47, 4, 231, 236, Aug. 2013, [Peer-reviewed]
English, Scientific journal

The Distribution of TRPV1 and TRPV2 in the rat pharynx
Rika Sasaki; Tadasu Sato; Takehiro Yajima; Mitsuhiro Kano; Toshihiko Suzuki; Hiroyuki Ichikawa
Cellular and Molecular Neurobiology, 33, 5, 707, 714, Jul. 2013, [Peer-reviewed]
English, Scientific journal

The Distribution of Transient Receptor Potential Melastatin-8 in the Rat Soft Palate, Epiglottis, and Pharynx
Tadasu Sato; Masatoshi Fujita; Mitsuhiro Kano; Hiroshi Hosokawa; Teruyoshi Kondo; Toshihiko Suzuki; Eriko Kasahara; Noriaki Shoji; Takashi Sasano; Hiroyuki Ichikawa
CELLULAR AND MOLECULAR NEUROBIOLOGY, 33, 2, 161, 165, Mar. 2013, [Peer-reviewed], [Lead author, Corresponding author]
English, Scientific journal

Osteocalcin- and Osteopontin-Containing Neurons in the Rat Hind Brain
Toshihiko Suzuki; Tadasu Sato; Hiroyuki Ichikawa
CELLULAR AND MOLECULAR NEUROBIOLOGY, 32, 8, 1265, 1273, Nov. 2012, [Peer-reviewed]
English, Scientific journal

Role of cytokines in lavage or drainage fluid after hemithyroidectomy in wound healing: Involvement of histamine in the acceleration and delay of wound healing
Miku Arai; Hiromi Ogita-Nakanishi; Koutetsu Lee; Katsuhiro Yoshimura; Ryo Kawata; Atsuko Kanazawa; Tetsuya Terada; Hiroshi Takenaka; Tadasu Sato; Yasuo Endo; Ryuji Kato; Yoshio Ijiri; Kazuhiko Tanaka; Junko Tashiro-Yamaji; Takahiro Kubota; Ryotaro Yoshida
WOUND REPAIR AND REGENERATION, 20, 2, 158, 165, Mar. 2012, [Peer-reviewed]
English, Scientific journal

Neuronal dystonin isoform 2 is a mediator of endoplasmic reticulum structure and function
Scott D. Ryan; Andrew Ferrier; Tadasu Sato; Ryan W. O'Meara; Yves De Repentigny; Susan X. Jiang; Sheng T. Hou; Rashmi Kothary
MOLECULAR BIOLOGY OF THE CELL, 23, 4, 553, 566, Feb. 2012, [Peer-reviewed]
English, Scientific journal

Peptide 19-containing neurons in the medullary dorsal horn, subnuclei interpolaris and oralis, and nucleus principalis of the rat
Toshihiko Suzuki; Tadasu Sato; Rika Sasaki; Hiroyuki Ichikawa
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER, 194, 4, 321, 328, 2012, [Peer-reviewed]
English, Scientific journal

Motor Unit Abnormalities in Dystonia musculorum Mice
Yves De Repentigny; Andrew Ferrier; Scott D. Ryan; Tadasu Sato; Rashmi Kothary
PLOS ONE, 6, 6, e21093, Jun. 2011, [Peer-reviewed]
English, Scientific journal

Masseteric Nerve Injury Increases Expression of Brain-Derived Neurotrophic Factor in Microglia Within the Rat Mesencephalic Trigeminal Tract Nucleus
Hiroyuki Ichikawa; Tadasu Sato; Mitsuhiro Kano; Toshihiko Suzuki; Saburo Matsuo; Hiroyasu Kanetaka; Yoshinaka Shimizu
CELLULAR AND MOLECULAR NEUROBIOLOGY, 31, 4, 551, 559, May 2011, [Peer-reviewed]
English, Scientific journal

Increase of CGRP Expression in Motor Endplates Within Fore and Hind Limb Muscles of the Degenerating Muscle Mouse (Scn8a (dmu) )
Tadasu Sato; Yoshinaka Shimizu; Mitsuhiro Kano; Toshihiko Suzuki; Hiroyasu Kanetaka; Leona W. G. Chu; Patrice D. Cote; Hidetoshi Shimauchi; Hiroyuki Ichikawa
CELLULAR AND MOLECULAR NEUROBIOLOGY, 31, 1, 155, 161, Jan. 2011, [Peer-reviewed], [Lead author]
English, Scientific journal

健康寿命を支える食・運動(摂る・動く・息する)の身体-細胞連携適応生化学基盤 摂る・動く・息するを支える口腔の力 口腔組織由来因子による神経保護機構
高橋 かおり; 吉田 卓史; 佐藤 匡; 若森 実, 日本生化学会大会プログラム・講演要旨集, 98回, 2S13e, 02, Nov. 2025
(公社)日本生化学会, Japanese

液状食の継続摂取により発育阻害されたラット下顎頭に対する固形食への変更が与える発育改善効果
田村 千景; 高橋 茂; 佐藤 匡; 八若 保孝, Journal of Oral Biosciences Supplement, 2025, 150, 150, Sep. 2025
(一社)歯科基礎医学会, Japanese

ラット三叉神経節におけるTRPM7の分布とその機能の解明
妻木 ひろか; 佐藤 匡; 福永 智広; 矢島 健大; 金高 弘恭, 日本矯正歯科学会大会プログラム・抄録集, 84回, 173, 173, Sep. 2025
(公社)日本矯正歯科学会, Japanese

機械刺激負荷歯根膜におけるTRPV2、Piezo 1を起点とするMe5保護作用を持つWnt5aの産生制御機構の解明(Wnt5a, produced in the mechanically stimulated periodontal ligament cells after TRPV2 and/or Piezo1 activation, maintains mesencephalic trigeminal nucleus neurons)
Takahashi Kaori; Yoshida Takashi; Sato Tadasu; Nakamura Takashi; Wakamori Minoru, The Journal of Physiological Sciences, 75, Suppl.1, 489, 489, Mar. 2025
(一社)日本生理学会, English

マウス三叉神経節におけるP2X3受容体の分布に関する免疫組織化学的研究(Immunohistochemical study on the distribution of P2X3 receptor in the trigeminal ganglion of newborn and adult mouse)
Sato Tadasu; Ishikawa Mika; Yajima Takehiro; Mizuta Kentaro, The Journal of Physiological Sciences, 75, Suppl.1, 455, 455, Mar. 2025
(一社)日本生理学会, English

ラット唾液腺におけるα-シヌクレイン陽性神経線維の分布
西谷登美子; 佐藤匡; 佐藤匡; 矢島健大; 矢島健大; 星加知宏; 西谷佳浩, 日本再生歯科医学会誌, 23, 1, 38, Mar. 2025

機械刺激を受けた歯根膜が産生するWnt5aの三叉神経中脳路核細胞への作用
高橋かおり; 吉田卓史; 佐藤匡; 中村卓史; 若森実, 日本薬理学雑誌, 160, Supplement, 2025

ラット実験的歯移動時における破骨細胞分化のTRPV2の制御因子としての役割
茂見 翔平; 佐藤 匡; 坂本 麻由里; 本田 貴洋; 妻木 ひろか; 出口 徹; 市川 博之; 福永 智広; 溝口 到, 日本矯正歯科学会大会プログラム・抄録集, 82回, 182, 182, Nov. 2023
(公社)日本矯正歯科学会, Japanese

ラット唾液腺支配の上唾液核ニューロンに対するドーパミンの影響
美藤純弘; 佐藤匡; 矢島健大; 堀江謙吾; 市川博之; 吉田竜介, Journal of Oral Biosciences Supplement (Web), 2023, 2023

ヒト小唾液腺における神経伝達物質の分布(Distribution of neurotransmitter agents in the human minor salivary glands)
佐藤 あゆみ; 矢島 健大; 市川 博之; 佐藤 匡, Journal of Oral Biosciences Supplement, 2022, 366, 366, Sep. 2022
(一社)歯科基礎医学会, Japanese

唾液腺支配の上唾液核ニューロンに対するドーパミンの影響
美藤純弘; 佐藤匡; 矢島健大; 小橋基; 市川博之; 吉田竜介, 日本栄養・食糧学会中国・四国支部大会講演要旨集(CD-ROM), 55th, 2022

ヒト中頸神経節の交感神経節後ニューロンにおける神経伝達物質合成酵素及び神経ペプチドの分布
佐藤 匡; 矢島 健大; 市川 博之, Journal of Oral Biosciences Supplement, 2020, 242, 242, Sep. 2020
(一社)歯科基礎医学会, Japanese

ラット感覚神経節におけるTRPM3の分布
矢島 健大; 佐藤 匡; 島崎 健一郎; 市川 博之, Journal of Oral Biosciences Supplement, 2020, 254, 254, Sep. 2020
(一社)歯科基礎医学会, Japanese

オレキシンは顎下腺・舌下腺支配の副交感神経節前ニューロンを興奮させる
美藤 純弘; 佐藤 匡; 矢島 健大; 藤田 雅子; 小橋 基; 市川 博之; 吉田 竜介, 日本栄養・食糧学会大会講演要旨集, 74回, 217, 217, Apr. 2020
(公社)日本栄養・食糧学会, Japanese

The nerve injury increases the expression of galanin in the trigeminal ganglion of Wistar rats
Fei Liu; Min Wang; Jie-fei Shen; Hiroyuki Ichikawa; Takehiro Yajima; Tadasu Sato, JOURNAL OF ANATOMY, 236, 195, 195, Apr. 2020
English, Summary international conference

The trigeminal ganglion in a spinal muscular atrophy mouse model
Kenichiro Shimazaki; Takehiro Yajima; Yves De Repentigny; Rashmi Kothary; Hiroyuki Ichikawa; Tadasu Sato, JOURNAL OF ANATOMY, 236, 203, 204, Apr. 2020
English, Summary international conference

ヒトにおける膝神経節ニューロンの分布
佐藤匡, 東北大学歯学雑誌, 37/39, 2/1, 2020

上唾液核ニューロンに対する摂食亢進ペプチドの影響
美藤 純弘; 佐藤 匡; 矢島 健大; 藤田 雅子; 小橋 基; 市川 博之; 吉田 竜介, 岡山歯学会雑誌, 38, 2, 88, 88, Dec. 2019
岡山歯学会, Japanese

頸部におけるalpha-synuclein含有感覚神経の分布と機能
佐藤 匡; 矢島 健大; 島崎 健一郎; 西谷 登美子; 星加 知宏; 西谷 佳浩; 市川 博之, Journal of Oral Biosciences Supplement, 2019, 375, 375, Oct. 2019
(一社)歯科基礎医学会, Japanese

唾液腺支配のラット上唾液核ニューロンに対する摂食亢進ペプチドの影響
美藤 純弘; 佐藤 匡; 藤田 雅子; 小橋 基; 市川 博之; 吉田 竜介, Journal of Oral Biosciences Supplement, 2019, 381, 381, Oct. 2019
(一社)歯科基礎医学会, Japanese

摂食亢進ペプチドのラット顎下腺・舌下腺支配の副交感神経節前ニューロンに対する影響
美藤 純弘; 佐藤 匡; 矢島 健大; 藤田 雅子; 小橋 基; 市川 博之; 吉田 竜介, 日本味と匂学会誌, 第53回大会Proceeding集, S55, S58, Sep. 2019
視床下部に存在する10種類の摂食亢進ペプチドについて、ラット顎下腺・舌下腺支配の上唾液核(SSN)ニューロンを興奮させるかどうか検討した。Wistar系ラット(6〜14日齢)を用いて、標識ニューロンからホールセルパッチクランプ法により膜電位や膜電流を記録し、摂食亢進ペプチドをバス投与したときの影響を調べた。膜電位に対する応答では、orexin(OX)-Aに対する応答は他のペプチドと比較して有意に大きく、活動電位を伴うものがあった。また過分極応答を誘発するものはなかった。膜の入力抵抗に対する影響では、コントロールと比較してOX-A存在下では、同じ通電量60pAで発火頻度が増加した。また、通電ごとの平均の静止膜電位はコントロールでは-66mVであったのに対して、OX-A存在下では-59mVへと浅くなった。また、300nM OX-Aにより、多くのSSNニューロンが内向き電流を誘発していた。, 日本味と匂学会, Japanese

ラット露髄歯におけるPituitary adenylate cyclase-activating polypeptideの分布
矢島 健大; 佐藤 匡; 齋藤 正寛; 市川 博之, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 149回, 108, 108, Oct. 2018
(NPO)日本歯科保存学会, Japanese

alpha-synucleinの感覚神経節における分布
西條 詩織; 佐藤 匡; 矢島 健大; 島崎 健一郎; 西谷 登美子; 星加 知宏; 西谷 佳浩; 市川 博之, Journal of Oral Biosciences Supplement, 2018, 346, 346, Sep. 2018
(一社)歯科基礎医学会, Japanese

顎下腺・舌下腺支配の上唾液核ニューロンに対する摂食促進性ペプチドの影響
美藤 純弘; 佐藤 匡; 藤田 雅子; 市川 博之; 小橋 基, Journal of Oral Biosciences Supplement, 2018, 406, 406, Sep. 2018
(一社)歯科基礎医学会, Japanese

Orexins excite parasympathetic preganglionic neurons in the superior salivatory nucleus innervating the salivary glands
Yoshihiro Mitoh; Tadasu Sato; Masako Fujita; Motoi Kobashi; Hiroyuki Ichikawa; Ryuji Matsuo, CHEMICAL SENSES, 41, 9, E267, E267, Nov. 2016
English, Summary international conference

ラット三叉神経節におけるTRPCの発現
藤田 雅俊; 佐藤 匡; 矢島 健大; 市川 博之, Journal of Oral Biosciences Supplement, 2016, 275, 275, Sep. 2016
(一社)歯科基礎医学会, Japanese

ヒト膝神経節における感覚ニューロンに関する研究
山隈優; 佐藤匡; 佐々木悠; 神田典幸; 笹原庸由; 国分壮一; 市川博之, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 121st, 145, 2016
Japanese

ヒト副鼻腔及び三叉神経節におけるCGRPとTRPV2の分布
笹原庸由; 佐藤匡; 神田典幸; 佐々木悠; 山隈優; 国分壮一; 市川博之, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 121st, 144, 2016
Japanese

ヒト星状神経節におけるTRPV1およびTRPV2の分布
藤井 勇記; 佐藤 匡; 清水 良央; 市川 博之; 国分 壮一; 小川 主税; 工藤 快; 後藤 宏樹, Journal of Oral Biosciences Supplement, 2015, 537, 537, Sep. 2015
(一社)歯科基礎医学会, Japanese

神経損傷後の口腔感覚機能回復を目指した研究最前線 眼窩下神経損傷における三叉神経節でのalpha-2/delta-1 subunitの分布と機能
佐藤 匡; 市川 博之, Journal of Oral Biosciences Supplement, 2015, 108, 108, Sep. 2015
(一社)歯科基礎医学会, Japanese

ラット口腔顔面領域におけるTRPM8陽性神経線維の分布
矢島 健大; 佐藤 匡; 市川 博之; 齋藤 正寛; 島内 英俊; 細川 浩; 近藤 照義, Journal of Oral Biosciences Supplement, 2015, 159, 159, Sep. 2015
(一社)歯科基礎医学会, Japanese

顎下腺・舌下腺を支配する上唾液核ニューロン活動の摂食中枢による調節
美藤 純弘; 佐藤 匡; 藤田 雅子; 小橋 基; 市川 博之; 松尾 龍二, 岡山歯学会雑誌, 33, 2, 46, 46, Dec. 2014
岡山歯学会, Japanese

ヒト迷走神経下神経節と咽頭の神経支配(Distribution of TRPVs, P2X3, and parvalbumin in the human nodose ganglion)
浦田 悠輔; 佐藤 匡; 岡島 多翔幸; 南野 雅一; 清水 良央; 市川 博之, Journal of Oral Biosciences Supplement, 2014, 139, 139, Sep. 2014
(一社)歯科基礎医学会, English

オレキシンはラット顎下唾液腺を支配する上唾液核ニューロンを興奮させる
美藤 純弘; 佐藤 匡; 藤田 雅子; 小橋 基; 市川 博之; 松尾 龍二, Journal of Oral Biosciences Supplement, 2014, 203, 203, Sep. 2014
(一社)歯科基礎医学会, Japanese

耳下腺・顎下腺を支配する感覚ニューロンにおけるTRPV1及びTRPV2の分布
佐藤匡; 市川博之, 日本解剖学会総会・全国学術集会講演プログラム・抄録集, 119th, 2014

ラット口腔顔面の痛みに対するendomorphin‐1の役割
矢島健大; 佐藤匡; 市川博之; 齋藤正寛, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 139回, 144, 144, Oct. 2013
(NPO)日本歯科保存学会, Japanese

ラット三叉神経節におけるendomorphin-1の分布 免疫組織化学的手法による検討
矢島 健大; 佐藤 匡; 齋藤 正寛; 市川 博之, Journal of Oral Biosciences Supplement, 2013, 147, 147, Sep. 2013
(一社)歯科基礎医学会, Japanese

ラット顎下腺・舌下腺を支配する上唾液核ニューロンに対するオレキシンの興奮作用
美藤 純弘; 佐藤 匡; 藤田 雅子; 小橋 基; 市川 博之; 松尾 龍二, Journal of Oral Biosciences Supplement, 2013, 179, 179, Sep. 2013
(一社)歯科基礎医学会, Japanese

ラット咽頭及びその周囲組織におけるTRPV1とTRPV2の分布
佐藤 匡; 矢島 健大; 狩野 充浩; 鈴木 敏彦; 市川 博之, Journal of Oral Biosciences Supplement, 2013, 182, 182, Sep. 2013
(一社)歯科基礎医学会, Japanese

ラット口腔顔面領域におけるTRPM8陽性神経線維の分布
矢島健大; 佐藤匡; 市川博之, Journal of Oral Biosciences Supplement (Web), 138回, 88, 88, May 2013
(NPO)日本歯科保存学会, Japanese

Dumマウスにおける骨格筋及び運動ニューロンの変性
藤田 雅俊; 佐藤 匡; 狩野 充浩; 清水 良央; 金高 弘恭; 鈴木 敏彦; 市川 博之, Journal of Oral Biosciences Supplement, 2012, 107, 107, Sep. 2012
(一社)歯科基礎医学会, Japanese

ラット軟口蓋、喉頭蓋及び咽頭におけるTRPM8の分布
佐藤 匡; 藤田 雅俊; 狩野 充浩; 鈴木 敏彦; 市川 博之, Journal of Oral Biosciences Supplement, 2012, 111, 111, Sep. 2012
(一社)歯科基礎医学会, Japanese

後肢筋萎縮を伴うDegenerating muscle(dmu) mouseの骨成長に関する形態計測学的研究
清水 良央; 金高 弘恭; 佐藤 匡; 市川 博之; 熊本 裕行, 日本病理学会会誌, 101, 1, 343, 343, Mar. 2012
(一社)日本病理学会, Japanese

摂る・動く・息するを支える口腔の力 ー 口腔組織由来因子による神経保護機構
髙橋 かおり; 吉田 卓史; 佐藤 匡; 若森 実
第98回日本生化学会大会, 04 Nov. 2025, Nominated symposium
[Invited]

口腔咽頭領域における伝達物質やセンサーに関する研究
佐藤 匡
令和6年度北海道歯学会秋季学術大会, 20 Nov. 2024, Invited oral presentation
[Invited]

Distribution and possible function of galanin in the rat dura mater
Tadasu Sato; Takehiro Yajima; Hiroyuki Ichikawa
VPAC ISBAP 2022, 31 Oct. 2022, English, Nominated symposium
30 Oct. 2022 - 02 Nov. 2022, [Invited]

口腔顔面領域における感覚伝達のメカニズムについて：三叉神経侵害受容ニューロンの形態と機能
市川 博之; 矢島 健大; 佐藤 匡
第126回日本解剖学会総会・全国学術集会／第98回日本生理学会大会, 29 Mar. 2021, Nominated symposium
[Invited]

ヒトにおける膝神経節ニューロンの分布
佐藤 匡
第73回東北大学歯学会, 08 Jun. 2018
[Invited]

眼窩下神経損傷における三叉神経節でのalpha-2/delta-1 subunitの分布と機能
佐藤 匡; 市川 博之
第57回歯科基礎医学会学術大会, 11 Sep. 2015
[Invited]

alpha-2/delta-1 subunit of dihydropyridine receptor in the rat trigeminal ganglion
佐藤 匡
第120回日本解剖学会総会・全国学術集会／第92回日本生理学会大会, 21 Mar. 2015
[Invited]

口腔構造解析学, 2024年, 博士後期課程, 歯学院

口腔構造解析学研究, 2024年, 博士後期課程, 歯学院

口腔機能解剖学, 2024年, 博士後期課程, 歯学院

口腔機能解剖学研究, 2024年, 博士後期課程, 歯学院

解剖学・口腔解剖学Ⅱ, 2024年, 学士課程, 歯学部

解剖学・口腔解剖学実習, 2024年, 学士課程, 歯学部

歯科から挑む神経変性制御：歯根膜由来因子と微量元素動態による脳恒常性機構の解明
科学研究費助成事業
01 Apr. 2026 - 31 Mar. 2029
高橋 かおり; 吉田 卓史; 佐藤 匡
日本学術振興会, 基盤研究(C), 東北大学, 26K12993

閉経女性における顎関節症の重症化予防と疼痛の抑制
科学研究費助成事業
01 Apr. 2026 - 31 Mar. 2029
市川 博之; 永山 哲聖; 佐藤 匡; 西谷 佳浩; 西谷 登美子
日本学術振興会, 基盤研究(C), 鹿児島大学, 26K14145

食を通じた新たな摂食嚥下リハビリテーション手法の開発
科学研究費助成事業
01 Apr. 2026 - 31 Mar. 2029
矢島 健大; 佐藤 匡; 高橋 茂
日本学術振興会, 基盤研究(C), 北海道大学, 26K14164

アルツハイマー病プレクリニカル期におけるジンジパインとNETsによる脳機能障害
科学研究費助成事業
01 Apr. 2025 - 31 Mar. 2028
多田 浩之; 佐藤 匡; 松下 健二
日本学術振興会, 基盤研究(C), 東北大学, 25K12985

モデル動物を用いたパーキンソン病初期における嚥下障害メカニズムの解明
科学研究費助成事業
01 Apr. 2024 - 31 Mar. 2027
佐藤 匡
日本学術振興会, 基盤研究(C), 東北大学, 24K12883

嚥下機能における匂い刺激の影響：誤嚥性肺炎の予防に向けて
科学研究費助成事業 基盤研究(C)
Apr. 2023 - Mar. 2026
矢島 健大; 佐藤 匡
日本学術振興会, 基盤研究(C), 東北大学, 23K10500

炎症性歯髄におけるイオンチャンネルの役割
科学研究費助成事業 基盤研究(C)
Apr. 2022 - Mar. 2026
西谷 登美子; 星加 知宏; 佐藤 匡; 矢島 健大; 西谷 佳浩
日本学術振興会, 基盤研究(C), 鹿児島大学, Coinvestigator, 22K10003

パーキンソン病初期におこる嚥下障害発生機序の解明
科学研究費助成事業 基盤研究(C)
Apr. 2021 - Mar. 2025
佐藤 匡
嚥下障害は、低栄養や誤嚥性肺炎などを引き起こす原因になるため、超高齢社会においては重要な問題となることもある。パーキンソン病は高齢者に多く発症する病気のひとつであり、脳内のニューロンの細胞体にalpha-synucleinが異常に凝集することが知られている。また、パーキンソン病ではその初期から嚥下障害が発症することも知られている。これまでに、ノーマルの動物を用いた研究で、迷走神経や舌咽神経の神経節の一次感覚ニューロンにalpha-synucleinが発現しており、咽頭やその周囲にalpha-synuclein陽性神経線維が分布していることを報告してきた。そこで本研究では、パーキンソン病における摂食嚥下障害の発生メカニズムの解明を目的としている。本年度はまず、alpha-synuclein陽性神経線維の分布を詳細に調べるためノーマルラットを用いて口腔およびその周囲におけるalpha-synuclein陽性神経線維の分布について免疫染色法を用いて調べた。その結果、唾液腺内にalpha-synuclein陽性神経線維が分布しており、それらの神経線維は血管周囲や腺房周囲にも分布していることがわかった。そこで、これらの神経線維の成分を調べるために各種抗体を用いて蛍光二重染色を実施したところ、alpha-synuclein陽性神経線維の一部は自律神経系由来である可能性が示唆された。そこで、唾液腺に分布する自律神経系の細胞体を有する自律神経節を確認し、その神経節についても免疫染色法ならびに蛍光二重染色法でニューロンでの発現分布を確認した。
日本学術振興会, 基盤研究(C), 東北大学, Principal investigator, 21K09853

中枢性筋萎縮症における嚥下障害とその改善
科学研究費助成事業 基盤研究(C)
Apr. 2021 - Mar. 2024
立谷 大介; 佐藤 匡
本申請課題は、中枢性筋萎縮症を含む多くの運動障害性疾患では嚥下に関する病態に不明な点が多いため、運動障害性疾患において嚥下運動にどのような影響をもたらすかを、中枢性筋萎縮症モデルマウスを用いて明らかにすることを目的としておこなわれている。さらには嚥下障害発生メカニズムの解明ならびに、その改善方法の確立を目標としている。摂食嚥下は、三叉神経・舌咽神経・迷走神経・舌下神経による感覚や運動の情報伝達及び調節により行われている。これまでの研究成果として、ワイルドタイプマウスと中枢性筋萎縮症モデルマウスを比較すると、中枢性筋萎縮症モデルマウスの三叉神経節では約３０％の感覚ニューロンが減少しており、特に大型のニューロンが減少している可能性があることを報告してきた。
そこで、本年度は中型から大型のニューロンが発現しているタンパクに着目し、その抗体を用いて免疫染色をおこなった。その後、陽性ニューロンの数の比較や陽性ニューロンの細胞体の大きさを計測し比較検討した。その結果、ワイルドタイプマウスに比べて中枢性筋萎縮症モデルマウスの三叉神経節では、陽性ニューロンの数が優位に減少していた。さらに、陽性ニューロンの細胞体の大きさについても分析したところ、中間報告として分布に変化がみられたが分析を終えることはできなかった。これまでの所見から、中枢性筋萎縮症モデルマウスでは三叉神経節の大型ニューロンに変性が生じている可能性が示唆された。
日本学術振興会, 基盤研究(C), 東北大学, Coinvestigator, 21K11188

The study of dysphagia on model mice for motor dysfunction
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Apr. 2018 - Mar. 2021
Tachiya Daisuke
Histochemical and immunohistochemical methods were performed on model mice for motor dysfunction; dystonin-knockout (dt) mouse and survival motor neuron (SMN) mice. At gloss anatomical level, the head and neck were small in dt and SMN mice compared to wild type mice. The size of the maxilla, mandible, tongue, pharynx and larynx in dt mice was smaller than in wildtype mice. And, the maxilla, mandible, lingual root and larynx were soft and fragile. The development of the cartage and bone in these regions is probably disrupted by the mutations. In addition, the size of the trigeminal ganglion (TG) in SMN mice severely decreased. At a microscopic level, the number of sensory neurons in the TG of SMN mice. In this study, it is suggested that the possibility of dysfunction in food intact and swallowing in dt and SMN mice. The disfunction may be due to nerve damages, muscular atrophy and hypocalcification in the head and neck regions.
Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Tohoku University, Coinvestigator, 18K10667

Investigation about swallowing dysfunction in muscular atrophy model animals
Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
Apr. 2016 - Mar. 2019
SATO Tadasu
Patients with motor impairment occasionally have swallowing dysfunction. Synuclin is a protein which is related to motor impairment diseases including Parkinson’s disease. In this study, we examined the distribution of alpha-synuclein nerve fibers in the pharynx. We also examined the alpha-syunclein were contained in small- to medium-sized neurons in the vagus and glossopharyngeal ganglia.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Tohoku University, Principal investigator, 16K20409

Study about mechanism of swallowing disorder in muscle atrophy disease
Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
Apr. 2014 - Mar. 2016
SATO TADASU
The cause of muscle atrophy diseases includes atrophy and degeneration of nerves and/or muscles. The swallowing disorder is known to be a severe symptom of these diseases.
In this study, we examined the changes of muscle and nerves in the pharynx and oral structures in models of muscle atrophy diseases. We also examined the glial reaction in the spinal cord of the models.
Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Tohoku University, Principal investigator, 26861541

The study of swallowing disorder in neuromuscular disease mice model
Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up
Aug. 2012 - Mar. 2014
SATO TADASU
The cause of muscle atrophy disease includes degeneration of nerves and/or muscles. The swallowing disorder is known to be derived from such disease.
In this study, we examined the distribution and origin of sensory nerves in the pharynx. We also examined the neural degeneration in neuromuscular disease mice model.
Japan Society for the Promotion of Science, Grant-in-Aid for Research Activity Start-up, Tohoku University, Principal investigator, 24890021

菌体成分、特にNOD1ならびにNOD2リガンドの鎮痛作用
科学研究費助成事業
2009 - 2011
佐藤 匡
運動失調症では、筋肉や神経の機能不全により運動障害が生じることが知られている。このような疾患では、しばしば筋や関節に疼痛が生じ、この疼痛がさらに随意的な運動を困難にしている。例えば筋変性モデルマウスは、四肢の運動不全のため疼痛行動を示すことができない。しかしながら、脊髄の後角において痛みのマーカーであるc-Fosの発現が示す2次ニューロンが増加する。以上のことから、このモデルマウスでは筋肉の変性とともに、筋や関節の異常な伸展や屈曲により疼痛が生じていると考えられる。したがって、運動失調症の治療においては、筋肉の運動機能を回復させるとともに、疼痛を軽減することが非常に重要であると考えられている。
自然免疫は、Toll-like receptor(TLR)やNOD-like receptor(NLR)が微生物に特徴的な構造を認識することにより発動する。1960～80年代に内毒素性リポ多糖(LPS)やペプチドグリカンの要構造にあたるムラミルジペプチド(MDP)の鎮痛作用が報告されている。昨年度までの研究においては、各種菌体成分を投与後における5%ホルマリン或いは、2%酢酸処置による疼痛行動の観察を行ってきた。その結果、各種菌体成分が鎮痛作用を有することを明らかにした。
本年度は、各種菌体成分をマウスに投与した筋変性モデルマウスの脊髄及び脳においてニューロンにおける痛みのマーカーであるc-Fosの発現分布を調べた。各種菌体成分を投与すると、生理食塩水投与よりも、明らかにモデルマウスの脊髄や脳におけるc-Fosの発現量が減少していた。以上の結果から運動失調症に伴う疼痛には、各種菌体成分が有効である可能性が示唆された。
日本学術振興会, 特別研究員奨励費, 東北大学, 09J01314