Researcher basic information

• 博士（歯学）, 北海道大学

• https://researchmap.jp/read0115390?lang=en

• https://orcid.org/0000-0002-1023-7680
• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200901071053231880
• https://www.den.hokudai.ac.jp/gen-dent/index.html
• https://www.den.hokudai.ac.jp/gen-dent/en.html

• 50372256

• https://orcid.org/0000-0002-1023-7680

• 200901071053231880

• コンピテンシー基盤型教育
• 根面う蝕
• Bioactive glass
• 骨補填材
• レーザー
• hydroxyapatite
• biomineralization
• calcium phosphate
• 光線力学療法
• Graphene oxide
• translational research
• antibacterial activity
• biomaterial
• nano carbon
• scaffold
• bone tissue engineering
• nano material
• periodontal tissue engineering

• Life Science, Biomaterials
• Life Science, Biomedical engineering
• Life Science, Regenerative dentistry and dental engineering
• Life Science, Conservative dentistry

• Bachelor's degree program, School of Dental Medicine
• Doctoral (PhD) degree program, Graduate school of Dental Medicine

Research activity information

• Jun. 2026, 日本歯科保存学会, 奨励賞
  酸化グラフェン超薄膜と口腔洗浄含嗽剤による抗菌性耐水コーティング
  西田絵利香;金本佑生実;石井花英;郷田 隼;宮治裕史
• Nov. 2023, 日本レーザー歯学会, 優秀ポスター賞
  過飽和液中レーザー照射法によるヒト歯根表面の機能化
  田中佐織;宮治裕史;大矢根綾子
• Sep. 2023, 大阪大学接合科学研究所, 接合科学共同利用・共同研究賞
  もみ殻由来シリカ複合ナノ粒子の創製と新奇光抗菌剤としての展開
  川崎英也;宮治裕史
• Nov. 2018, 日本再生歯科医学会, 優秀発表賞
  カーボンナノチューブによるチタン-生体界面のナノスケール改質と骨形成接合効果
  西田絵利香;宮治裕史;蔀 佳奈子;眞弓佳代子;菅谷 勉;梅田純子;近藤勝義;古月文志
• Jun. 2018, 日本歯科保存学会, 奨励賞
  Antimicrobial photodynamic activity and cytocompatibility of Au25(Capt)18 clusters photoexcited by blue LED light irradiation
  S Miyata;H Miyaji;H Kawasaki;M Yamamoto;E Nishida;H Takita;T Akasaka;N Ushijima;T Iwanaga;T Sugaya
• May 2018, 日本バイオマテリアル学会北海道ブロック研究会, ポスター賞
  S-PRGナノフィラーの抗菌性バイオアクティブ効果とイヌ歯周病モデルへの応用
  眞弓佳代子;宮治裕史;降旗友和;金本佑生実;宮田さほり;蔀 佳奈子;赤坂 司;菅谷 勉
• May 2018, 北海道歯学会, 北海道歯学会賞
  Antimicrobial photodynamic activity and cytocompatibility of Au25(Capt)18 clusters photoexcited by blue LED light irradiation
  S Miyata;H Miyaji;H Kawasaki;M Yamamoto;E Nishida;H Takita;T Akasaka;N Ushijima;T Iwanaga;T Sugaya
• Nov. 2017, 日本総合歯科学会, 優秀賞
  レーザー援用バイオミメティック法によるレジン表面の改変とアパタイト形成能評価
  田中佐織;宮治裕史;西田絵利香;A. Joseph;NATHANAEL;中村真紀;大矢根綾子;田中 享;飯田俊二;高師則行;井上 哲
• Aug. 2017, 日本歯科保存学会, 奨励賞
  Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells
  S Yoshida;N Wada;D Hasegawa;H Miyaji;H Mitarai;A Tomokiyo;S Hamano;H Maeda
• May 2017, 日本バイオマテリアル学会北海道ブロック研究会, ポスター賞
  貴金属クラスターを用いた抗菌的光線力学療法の開発
  岡本一絵;宮治裕史;宮田さほり;川﨑英也;蔀 佳奈子;赤坂 司;菅谷 勉
• Mar. 2017, 田中貴金属記念財団, 奨励賞
  貴金属ナノクラスターと有機系光触媒複合による相乗効果を利用した新規歯周病光治療法の開発
  宮治裕史
• Sep. 2016, 大阪大学接合研究所, 接合科学・共同利用共同研究賞
  単分散CNTと金属の接合界面構造解析と表面多機能化
  宮治裕史;西田絵利香
• Sep. 2015, 日本歯科医学会, 優秀発表賞
  歯表面での3D微細構造構築によるバイオミメティック次世代歯科治療へ向けて
  赤坂 司;加我公行;宮治裕史;横山敦郎;吉田靖弘
• 2009, 韓国歯周病学会, KACD and JSCD 優秀賞
  Bone Marrow Cell Sheet in BMP-applied Collagen Scaffold for Bone Tissue Engineering
  K Inoue;H Miyaji;H Fukuda;H Yokoyama;S Tanaka;T Sugaya;M Kawanami
• 2006, 日本歯周病学会, 会誌賞
  BMP-2処理した象牙質表面におけるセメント質様硬組織形成のビスフォスフォネートによる抑制
  本郷哲也;宮治裕史;菅谷 勉;川浪雅光, Japan

• 歯内療法における材料概念と生体調和封鎖：生体適合性の視点から
  宮治裕史
  日誌保存誌, 69, 2, 95, 99, May 2026, [Invited], [Lead author, Corresponding author]
  Japanese, Symposium
• Evaluation of the Difference in Stress Distribution Patterns on the Condyle Between Skeletal Classes Using CTOAM
  Kazuhiro Matsushita; Yuichiro Matsui; Kaori Endo; Hirofumi Miyaji; Norimasa Iwasaki
  Journal of Maxillofacial and Oral Surgery, 07 Mar. 2026, [Peer-reviewed]
  Scientific journal
• Authors' Reply "Histochemical Assessment of Cementum and Alveolar Bone in SDT fa/fa Rat Mimicking Type II Diabetes".
  Tomoka Hasegawa; Mako Sakakibara; Xuanyu Liu; Mai Haraguchi-Kitakamae; Weisong Li; Haoyu Wang; Yan Shi; Jiaxin Cui; Hiromi Hongo; Tomomaya Yamamoto; Miki Abe-Takagi; Haruhi Maruoka; Hirofumi Miyaji; Minqi Li; Norio Amizuka
  Oral diseases, 04 Jan. 2026, [Peer-reviewed], [International Magazine]
  English
• Stage-Based Surgical Strategies and Outcomes for Preiser Disease: A Single-Center Experience.
  Hiroaki Kida; Yuichiro Matsui; Akio Minami; Daisuke Kawamura; Takeshi Endo; Daisuke Momma; Tsutomu Endo; Hirofumi Miyaji; Norimasa Iwasaki
  Hand (New York, N.Y.), 15589447251391379, 15589447251391379, 01 Dec. 2025, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND: The optimal surgical approach for Preiser disease, a rare avascular necrosis of the scaphoid, remains unclear. Our institution employs a stage-based strategy: vascularized bone grafting (VBG) or closing radial wedge osteotomy (CRWO) for early disease (Herbert classification stages 1-2) and proximal row carpectomy (PRC) for advanced disease (stages 3-4). This study evaluates this stage-based surgical approach. METHODS: We retrospectively analyzed 8 patients (mean age: 60.6 years) who underwent surgical treatment for Preiser disease between 2000 and 2019. Preoperative evaluation included Herbert and Kalainov classifications. Outcome measures included the range of motion (ROM), grip strength (GS), pain (numeric rating scale [NRS] score), Modified Mayo Wrist Score (MMWS), and radiological parameters. Mean follow-up was 50 months. RESULTS: Five patients had stage 2, 1 had stage 3, and 2 had stage 4 disease. Seven patients were treated according to our strategy; 1 stage 4 patient deviated. In those treated per protocol, the VBG/CRWO group (n = 5) and the PRC group (n = 2) showed improvements across all clinical outcomes. Despite clinical gains, radiographic disease progression occurred in 4 of the 5 patients in the VBG/CRWO group. The patient treated outside the standard approach experienced clinical deterioration and developed dorsal intercalated segment instability deformity. CONCLUSION: Our stage-based surgical strategy for Preiser disease yields favorable clinical outcomes. While radiographic progression may occur regardless of treatment, clinical improvements are generally maintained when procedures are appropriately selected based on disease stage.
• Antibacterial crystalline bilayers constructed on dentin substrates via laser-assisted mineralization: characterization and functional implications
  Kazuo Onuma; Miyabi Makino; Maki Nakamura; Erika Nishida; Kanako Shitomi; Saori Tanaka; Hirofumi Miyaji; Ayako Oyane
  Applied Surface Science, 711, 164117, 164117, Elsevier BV, Dec. 2025, [Peer-reviewed]
  Scientific journal
• Histochemical Assessment of Cementum and Alveolar Bone in SDT fa/fa Rat Mimicking Type II Diabetes.
  Tomoka Hasegawa; Mako Sakakibara; Xuanyu Liu; Mai Haraguchi-Kitakamae; Weisong Li; Haoyu Wang; Yan Shi; Jiaxin Cui; Hiromi Hongo; Tomomaya Yamamoto; Miki Abe-Takagi; Haruhi Maruoka; Hirofumi Miyaji; Minqi Li; Norio Amizuka
  Oral diseases, 31 Aug. 2025, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVE: To investigate the histopathology of diabetic periodontal tissues, we examined periodontal disease in Torii-Leprfa (SDT fa/fa) rats, mimicking type 2 diabetes. METHODS: Forty 30-week-old male SDT fa/fa rats and age-matched Sprague-Dawley (SD) rats were fixed, and the mandibular first molars and their periodontal tissues were histochemically examined. RESULTS: SDT fa/fa rats exhibited epithelial downgrowth in the previous region of interradicular/interalveolar septa and periodontal spaces. Many osteoclasts and osteoblasts were present on the alveolar bone, indicating high bone turnover. Masses of bacteria were predominantly observed in the necrotic dental pulps and the cementum, where most cementocytic lacunae were empty. The cementum of the SDT fa/fa rats showed a distinct immunolocalization of osteocalcin, osteopontin, and dentin matrix protein-1 compared with the SD rats, but did not show advanced glycation end products, which were instead detected in some blood vessels and interstitial fibrous tissues associated with the down-growing epithelium. CONCLUSIONS: SDT fa/fa rats exhibited necrosis of dental pulp and severe periodontitis featuring markedly diminished alveolar bone because of stimulated bone resorption and formation. In addition, dead cementum may serve as bacterial nests, leading to changes in the localization of cementum matrix proteins.
• Sema3A-and ascorbate-functionalized apatite-collagen scaffolds for enhanced bone regeneration
  Kaushita Banerjee; Hanae Ishii; Ayako Oyane; Maki Nakamura; Tomoya Inose; Erika Nishida; Kari Tsukita; Tomoka Hasegawa; Hirofumi Miyaji
  MRS Communications, 21 Aug. 2025, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Integration of dual drugs into a collagen scaffold by a combination of apatite coating and impregnation with apatite particles for periodontal regeneration.
  Kaushita Banerjee; Ayako Oyane; Maki Nakamura; Tomoya Inose; Erika Nishida; Kanako Shitomi; Hirofumi Miyaji
  RSC advances, 15, 24, 19480, 19488, 04 Jun. 2025, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Bioresorbable porous scaffolds capable of promoting osteoregeneration while preventing bacterial infection are needed for regenerative periodontal therapy. Previously, a porous collagen sponge coated with low-crystalline apatite has been shown to possess superior bioresorption and osteogenic properties compared to the uncoated sponge. In this study, we integrated osteogenic and antibacterial dual drugs into the sponge utilizing two types of apatite matrices to achieve further functionalization. First, the collagen sponge was coated with apatite loaded with an osteogenic drug, l-ascorbic acid 2-phosphate (AS), using a metastable supersaturated calcium phosphate (CaP) solution supplemented with AS. Second, the coated sponge was impregnated with apatite particles loaded with an antibacterial drug, ciprofloxacin (CF), which were fabricated using a labile supersaturated CaP solution supplemented with CF. The resulting dual drug-immobilized sponge demonstrated biological activities arising from both AS and CF; it enhanced proliferation of osteoblastic MC3T3-E1 cells and exhibited antibacterial activity against the oral bacterium Actinomyces naeslundii. The proposed technique to fabricate multifunctional scaffolds would offer a solution to provide more effective, patient-tailored regenerative periodontal therapy.
• Antimicrobial Water-resistant Coating Method by Compositing Graphene Oxide Ultrathin Film and Mouthwash Solution
  西田絵利香; 金本佑生実; 石井花英; 郷田隼; 宮治裕史
  日本歯科保存学雑誌(Web), 68, 3, 103, 112, (NPO)日本歯科保存学会, Jun. 2025, [Peer-reviewed], [Corresponding author]
  Japanese
• Long-Term Outcomes of Radial Shortening Osteotomy for Kienböck Disease: Minimum 20-Year Follow-Up Study.
  Tomoaki Suzuki; Yuichiro Matsui; Daisuke Momma; Takeshi Endo; Hirofumi Miyaji; Norimasa Iwasaki
  The Journal of hand surgery, 27 May 2025, [Peer-reviewed], [International Magazine]
  English, Scientific journal, PURPOSE: We have been performing radial shortening osteotomies for patients with negative ulnar variance and Lichtman stage 2 or higher Kienböck disease. Although we previously reported the results of this procedure 10 years after surgery, reports on follow-up beyond 10 years remain scarce. This study aimed to investigate the results of radial shortening osteotomy for Kienböck disease based on long-term clinical and radiographic outcomes for a minimum of 20 years after surgery. METHODS: The cohort comprised seven patients with eight wrists treated between 1991 and 2002, whose average age at the time of surgery was 25.9 years (range: 17-44 years). The preoperative Lichtman classification was stage 3A in one wrist, 3B in five, and stage 4 in one; the mean preoperative ulnar variance was -2.2 mm (range: -1.0 to -3.5 mm). Changes in pain, range of motion, grip strength, modified Mayo Wrist Score, Disabilities of the Arm, Shoulder, and Hand (DASH) score, and radiographs were evaluated from 10 years after surgery to the last follow-up at least 20 years after surgery. RESULTS: Pain remained reduced in all patients at 20 years after surgery. Improvements in wrist extension and flexion observed 10 years after surgery were maintained at the last follow-up. Grip strength at 10 years after surgery was maintained at the last follow-up. The mean modified Mayo wrist score and the mean DASH score were maintained from 10 years after surgery to the last follow-up. Radiography showed no progression of lunate collapse in any case, although one case showed progression of degeneration in the radiocarpal joint and the distal radioulnar joint. CONCLUSIONS: Good clinical results observed in patients 10 years after radial shortening osteotomy are likely to remain stable at 20 years after surgery. TYPE OF STUDY/LEVEL OF EVIDENCE: Therapeutic V.
• 【抗菌性・抗ウイルス性バイオマテリアル アップデート】酸化グラフェン超薄膜の導入による抗菌剤の基材定着性の向上
  宮治 裕史; 西田 絵利香; 郷田 隼; 川崎 英也
  バイオマテリアル-生体材料-, 43, 2, 152, 157, 日本バイオマテリアル学会, Apr. 2025, [Lead author]
  Japanese
• Fluoride-incorporated apatite coating on resin-based composite via laser-assisted rapid pseudo-biomineralization
  Nandha Kumar Ponnusamy; Ayako Oyane; Maki Nakamura; Tomoya Inose; Kazuo Onuma; Erika Nishida; Hirofumi Miyaji
  Applied Surface Science, 681, 161473, 161473, Elsevier BV, Feb. 2025, [Peer-reviewed]
  Scientific journal
• One-Step Fabrication of Water-Dispersible Calcium Phosphate Nanoparticles with Immobilized Lactoferrin for Intraoral Disinfection
  Maki Nakamura; Ayako Oyane; Tomoya Inose; Yukimi Kanemoto; Hirofumi Miyaji
  International Journal of Molecular Sciences, 26, 2, 20 Jan. 2025, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Lactoferrin is a highly safe antibacterial protein found in the human body and in foods. Calcium phosphate (CaP) nanoparticles with immobilized lactoferrin could therefore be useful as intraoral disinfectants for the prevention and treatment of dental infections because CaP is a mineral component of human teeth. In this study, we fabricated CaP nanoparticles with co-immobilized lactoferrin and heparin using a simple one-step coprecipitation process. Heparin, a negatively charged polysaccharide, was used as both an immobilizing agent for lactoferrin and a particle-dispersing agent. The immobilization efficiency for lactoferrin in the CaP nanoparticles depended on the concentrations of both the lactoferrin and heparin in the reaction solution and was over 90% under optimal conditions. The nanoparticles had a hydrodynamic diameter of about 150-200 nm and could be well dispersed in water, owing to their relatively large negative zeta potential derived from heparin. They were found to exhibit antibacterial activity against Actinomyces naeslundii, which is involved in the initial formation of dental plaque that consequently leads to dental caries and periodontal disease. These results indicate the potential of the proposed nanoparticles as intraoral disinfectants.
• Ultrafast coating of fluoride-substituted hydroxyapatite layers on teeth by laser-assisted crystallization: Comparison of surface structure and composition among enamel, dentin, and cementum
  Kazuo Onuma; Miyabi Makino; Ikuko Sakamaki; Maki Nakamura; Erika Nishida; Saori Tanaka; Hirofumi Miyaji; Ayako Oyane
  Applied Surface Science, 674, 15 Nov. 2024, [Peer-reviewed]
  Scientific journal
• A case of 6-year-old girl performed collaborative treatment for dentigerous cyst with three impacted teeth
  高師則行; 月田佳李; 飯田俊二; 田中佐織; 宮治裕史
  日本総合歯科学会雑誌(Web), 16, Oct. 2024, [Peer-reviewed]
• Natural Recovery of Very Limited Elbow Flexion After Rare Lateral Humeral Condylar Fracture in a 4-Year-Old Boy
  Koki Ohta; Yuichiro Matsui; Hiroyuki Kato; Daisuke Kawamura; Takeshi Endo; Daisuke Momma; Tamotsu Kamishima; Tatsunori Horie; Hirofumi Miyaji; Norimasa Iwasaki
  JBJS Case Connector, 14, 4, Oct. 2024, [Peer-reviewed], [International Magazine]
  English, Scientific journal, CASE: A 4-year-old boy developed malunion after open reduction and internal fixation (ORIF) of a rare lateral condylar humeral fracture running from the lateral humerus, through the trochlear cartilage, to the medial trochlea. Five months after ORIF, the active elbow flexion was limited to 50°. Magnetic resonance imaging (MRI) showed a step-off of the anterior trochlear cartilage, causing the limited flexion. After conservative observation for 5 years, the flexion improved to 135° and MRI showed that the step-off had disappeared. CONCLUSION: This case indicates that it is possible for pediatric patients to recover their range of motion following malunion of an elbow fracture.
• Fabrication of Ciprofloxacin-Immobilized Calcium Phosphate Particles for Dental Drug Delivery
  Aniruddha Pal; Ayako Oyane; Tomoya Inose; Maki Nakamura; Erika Nishida; Hirofumi Miyaji
  Materials, 17, 9, 26 Apr. 2024, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Calcium phosphate (CaP) particles immobilizing antibacterial agents have the potential to be used as dental disinfectants. In this study, we fabricated CaP particles with immobilized ciprofloxacin (CF), a commonly prescribed antibacterial agent, via a coprecipitation process using a supersaturated CaP solution. As the aging time in the coprecipitation process increased from 2 to 24 h, the CaP phase in the resulting particles transformed from amorphous to low-crystalline hydroxyapatite, and their Ca/P elemental ratio, yield, and CF content increased. Despite the higher CF content, the particles aged for 24 h displayed a slower release of CF in a physiological salt solution, most likely owing to their crystallized matrix (less soluble hydroxyapatite), than those aged for 2 h, whose matrix was amorphous CaP. Both particles exhibited antibacterial and antibiofilm activities along with an acid-neutralizing effect against the major oral bacteria, Streptococcus mutans, Porphyromonas gingivalis, and Actinomyces naeslundii, in a dose-dependent manner, although their dose-response relationship was slightly different. The aging time in the coprecipitation process was identified as a governing factor affecting the physicochemical properties of the resulting CF-immobilized CaP particles and their functionality as a dental disinfectant.
• Rapid regeneration of artificial enamel using laser-assisted biomineralization: With and without mesoscale hierarchy in hydroxyapatite rods
  Kazuo Onuma; Pal Aniruddha; Miyabi Makino; Ikuko Sakamaki; Maki Nakamura; Saori Tanaka; Hirofumi Miyaji; Ayako Oyane
  Materials Today Communications, 38, 108030, 108030, Elsevier BV, Mar. 2024, [Peer-reviewed]
  Scientific journal
• Fluoride-Incorporated Apatite Coating on Collagen Sponge as a Carrier for Basic Fibroblast Growth Factor
  Aniruddha Pal; Ayako Oyane; Maki Nakamura; Kenji Koga; Erika Nishida; Hirofumi Miyaji
  International Journal of Molecular Sciences, 25 Jan. 2024, [Peer-reviewed]
  Scientific journal
• Water-resistant transparent coating with nanocarbon material ′′graphene oxide′′ and antibacterial modification of the tooth surface
  宮治裕史
  北海道歯学雑誌, 44, 6, 9, 北海道歯学会, Sep. 2023, [Invited], [Lead author]
  Japanese
• Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells.
  Shoko Fujino; Sayuri Hamano; Atsushi Tomokiyo; Risa Sugiura; Daiki Yamashita; Daigaku Hasegawa; Hideki Sugii; Shinsuke Fujii; Tomohiro Itoyama; Hirofumi Miyaji; Hidefumi Maeda
  Scientific reports, 13, 1, 5668, 5668, 06 Apr. 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Conventional direct pulp-capping materials induce pulp cells to secrete various biomolecules in pulp tissues that promote reparative dentin formation through induction of odontoblastic differentiation of dental pulp stem cells (DPSCs). However, these biomolecules sometimes induce bone-like dentin with poor sealing properties. Therefore, exploration of biomolecules that allow tight sealing by tubular reparative dentin is required. We recently reported that dopamine (DA) is involved in dentinogenesis. Hence, we investigated the effect of DA on odontoblastic differentiation of DPSCs and reparative dentin formation. Both tyrosine hydroxylase (TH), a DA synthetase, and DA were expressed in odontoblast-like cells in vivo. In vitro, their expression was increased during odontoblastic differentiation of DPSCs. Furthermore, TH-overexpressing DPSCs had promoted odontoblastic differentiation and DA production. Moreover, DA stimulation promoted their differentiation and induced tubular reparative dentin. These results suggest that DA produced by TH is involved in odontoblastic differentiation of DPSCs and has an inductive capacity for reparative dentin formation similar to primary dentin. This study may lead to the development of therapy to preserve vital pulp tissues.
• 酸化亜鉛ユージノール系ペーストタイプ根管充填シーラーの材料特性ならびに生体親和性評価
  加藤 昭人; 宮治 裕史; 金本 佑生実; 蔀 佳奈子; 岡本 一絵; 吉野 友都; 浜本 朝子; 西田 絵利香; 菅谷 勉; 田中 佐織
  日本歯科保存学雑誌, 66, 2, 114, 123, (NPO)日本歯科保存学会, Apr. 2023, [Peer-reviewed], [Corresponding author]
  Japanese
• Water-resistant antibacterial properties of a graphene oxide/cetylpyridinium chloride complex formed on medical gauze fibers.
  Yukimi Kanemoto; Hirofumi Miyaji; Erika Nishida; Asako Hamamoto; Tsutomu Sugaya; Syun Gohda; Hironobu Ono
  Journal of oral biosciences, 65, 2, 202, 205, 19 Feb. 2023, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, OBJECTIVES: Graphene oxide (GO) is a nanocarbon material with a high aspect ratio (width:thickness) and abundant anionic functional groups on its surface. In this study, we attached GO to the surface of medical gauze fibers, constructed a complex with a cationic surface active agent (CSAA), and demonstrated that the treated gauze exhibits antibacterial activity even after rinsing with water. METHODS: Medical gauze was immersed in GO dispersion (0.001%, 0.01%, and 0.1%), rinsed with water, dried, and subjected to the Raman spectroscopy analysis. Subsequently, the gauze treated with 0.001% GO dispersion was immersed in 0.1% cetylpyridinium chloride (CPC) solution, immediately rinsed with water, and dried. Untreated, GO-only, and CPC-only gauzes were prepared for comparison. Each gauze was placed in a culture well, seeded with Escherichia coli or Actinomyces naeslundii, and turbidity was measured after 24 h of incubation. RESULTS: The Raman spectroscopy analysis of the gauze after immersion and rinsing showed a G-band peak, indicating that GO remained on the surface of the gauze. The turbidity measurements indicated that GO/CPC-treated gauze (GO-treated and rinsed, followed by CPC-treatment and rinsing) significantly decreased turbidity compared to the other gauzes (P<0.05), suggesting that the GO/CPC complex remained on the gauze fibers even after water rinsing and showed antibacterial activity. CONCLUSIONS: The GO/CPC complex imparts water-resistant antibacterial properties to gauze and has the potential to be widely used for the antimicrobial treatment of clothes.
• Rose bengal-decorated rice husk-derived silica nanoparticles enhanced singlet oxygen generation for antimicrobial photodynamic inactivation.
  Nanase Mori; Hideya Kawasaki; Erika Nishida; Yukimi Kanemoto; Hirofumi Miyaji; Junko Umeda; Katsuyoshi Kondoh
  Journal of materials science, 58, 6, 1, 13, 25 Jan. 2023, [Peer-reviewed], [International Magazine]
  English, Scientific journal, UNLABELLED: Rice husks are well known for their high silica content, and the RH-derived silica nanoparticles (RH NPs) are amorphous and biocompatible; therefore, they are suitable raw materials for biomedical applications. In this study, rose bengal-impregnated rice husk nanoparticles (RB-RH NPs) were prepared for their potential photosensitization and 1O2 generation as antimicrobial photodynamic inactivation. RB is a halogen-xanthene type's photosensitizer showing high singlet oxygen efficiency, and the superior photophysical properties are desirable for RB in the antimicrobial photodynamic inactivation of bacteria. To enhance the binding of anionic RB to RH NPs, we conducted cationization for the RH NPs using polyethyleneimine (PEI). The control of the RB adsorption state on cationic PEI-modified RH NPs was essential for RB RH-NP photosensitizers to obtain efficient 1O2 generation. Minimizing RB aggregation allowed highly efficient 1O2 production from RB-RH NPs at the molar ratio of RB with the PEI, XRB/PEI. = 0.1. The RB-RH NPs have significant antimicrobial activity against Streptococcus mutans compared to free RB after white light irradiation. The RB-RH NP-based antimicrobial photodynamic inactivation can be employed effectively in treating Streptococcus mutans for dental applications. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10853-023-08194-z.
• Periodontal tissue regeneration by recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles.
  Yuto Yoshino; Hirofumi Miyaji; Erika Nishida; Yukimi Kanemoto; Asako Hamamoto; Akihito Kato; Tsutomu Sugaya; Tsukasa Akasaka
  Journal of oral biosciences, 65, 1, 62, 71, 17 Jan. 2023, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, OBJECTIVES: Recombinant human collagen peptide (RCP) is a recombinantly created xeno-free biomaterial enriched in arginine-glycine-aspartic acid sequences with good processability whose use for regenerative medicine applications is under investigation. The biocompatibility and osteogenic ability of RCP granules combined with β-tricalcium phosphate (TCP) submicron particles (β-TCP/RCP) were recently demonstrated. In the present study, β-TCP/RCP was implanted into experimental periodontal tissue defects created in beagles to investigate its regenerative effects. METHODS: An RCP solution was lyophilized, granulated, and thermally cross-linked into particles approximately 1 mm in diameter. β-TCP dispersion (1 wt%; 500 μL) was added to 100 mg of RCP granules to form β-TCP/RCP. A three-walled intrabony defect (5 mm × 3 mm × 4 mm) was created on the mesial side of the mandibular first molar and filled with β-TCP/RCP. RESULTS: A micro-computed tomography image analysis performed at 8 weeks postoperative showed a significantly greater amount of new bone after β-TCP/RCP grafting (2.2-fold, P<0.05) than after no grafting. Histological findings showed that the transplanted β-TCP/RCP induced active bone-like tissue formation including tartaric acid-resistant acid phosphatase- and OCN-positive cells as well as bioabsorbability. Ankylosis did not occur, and periostin-positive periodontal ligament-like tissue formation was observed. Histological measurements performed at 8 weeks postoperative revealed that β-TCP/RCP implantation formed 1.7-fold more bone-like tissue and 2.1-fold more periodontal ligament-like tissue than the control condition and significantly suppressed gingival recession and epithelial downgrowth (P<0.05). CONCLUSIONS: β-TCP/RCP implantation promoted bone-like and periodontal ligament-like tissue formation, suggesting its efficacy as a periodontal tissue regenerative material.
• Optically Controlled Pseudo-biomineralization for Tooth Surface Functionalization
  大矢根綾子; 奈良崎愛子; 中村真紀; 宮治裕史
  セラミックス, 58, 9, 572, 575, 2023, [Invited]
• Fluoridated Apatite Coating on Human Dentin via Laser-Assisted Pseudo-Biomineralization with the Aid of a Light-Absorbing Molecule
  Ayako Oyane; Ikuko Sakamaki; Maki Nakamura; Kenji Koga; Kanako Shitomi; Saori Tanaka; Hirofumi Miyaji
  International Journal of Molecular Sciences, 23, 24, 15981, 15981, MDPI AG, 15 Dec. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal, A simple, area-specific coating technique for fluoridated apatite (FAp) on teeth would be useful in dental applications. Recently, we achieved area-specific FAp coating on a human dentin substrate within 30 min by a laser-assisted biomimetic (LAB) process; pulsed Nd:YAG laser irradiation in a fluoride-containing supersaturated calcium phosphate solution (FCP solution). The LAB-processed, FAp-coated dentin substrate exhibited antibacterial activity against a major oral bacterium, Streptococcus mutans. In the present study, we refined the LAB process with a combination of a dental diode laser and a clinically approved light-absorbing molecule, indocyanine green (ICG). A micron-thick FAp layer was successfully formed on the dentin surface within only 3 min by the refined LAB process, i.e., dental diode laser irradiation in the FCP solution following ICG treatment. The ICG layer precoated on the dentin substrate played a crucial role in inducing rapid pseudo-biomineralization (FAp layer formation) on the dentin surface by absorbing laser light at the solid-liquid interface. In the refined LAB process, the precoated ICG layer was eliminated and replaced with the newly formed FAp layer composed of vertically oriented pillar-like nanocrystals. Cross-sectional ultrastructural analysis revealed a smooth interface between the FAp layer and the dentin substrate. The refined LAB process has potential as a tool for the tooth surface functionalization and hence, is worth further process refinement and in vitro and in vivo studies.

  
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• In vivo Assessment of Tissue Compatibility of Root Canal Sealer Containing Surface Pre-reacted Glass-ionomer Fillers
  Saori TANAKA; Hirofumi MIYAJI; Kayoko MAYUMI; Yukimi K ANEMOTO; Erika NISHIDA
  Operative Dentistry, Endodontology and Periodontology, 2, 1, 33, 39, Dec. 2022, [Peer-reviewed]
  English, Scientific journal
• Sustained antibacterial coating with graphene oxide ultrathin film combined with cationic surface-active agents in a wet environment.
  Hirofumi Miyaji; Yukimi Kanemoto; Asako Hamamoto; Kanako Shitomi; Erika Nishida; Akihito Kato; Tsutomu Sugaya; Saori Tanaka; Natsuha Aikawa; Hideya Kawasaki; Syun Gohda; Hironobu Ono
  Scientific reports, 12, 1, 16721, 16721, 18 Oct. 2022, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, Antimicrobial surfactants contained in mouthrinse have excellent efficacy, but are not retained on the tooth surface (are rinsed away) due to their low water resistance and thus do not exhibit sustained antibacterial activity. We have developed a new coating method using graphene oxide (GO) that retains the surfactant on the tooth surface even after rinsing with water, thus providing a sustained antibacterial effect. Ultra-thin films of GO and an antimicrobial agent were prepared by (1) applying GO to the substrate surface, drying, and thoroughly rinsing with water to remove excess GO to form an ultrathin film (almost a monolayer, transparent) on the substrate surface, then (2) applying antimicrobial cationic surface active agents (CSAAs) on the GO film to form a composite coating film (GO/CSAA). GO/CSAA formation was verified by scanning electron microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy, and ζ-potential and contact angle measurements. GO/CSAA was effective at inhibiting the growth of oral pathogens for up to 7 days of storage in water, and antibacterial activity was recovered by reapplication of the CSAA. Antibacterial GO/CSAA films were also formed on a tooth substrate. The results suggest that GO/CSAA coatings are effective in preventing oral infections.

  
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• Fabrication and cell behavior assessment of antibacterial micro/nano-patterned chitosan films
  E Nishida; H Miyaji; T Sugaya; T Akasaka
  Nano Biomed, 14, 1, 1, 8, Jun. 2022, [Peer-reviewed], [Corresponding author]
  English
• In Vivo Inflammatory Effects and Surface Composition Changes in Implanted Root Canal Sealer Containing Bioactive Glass
  Akihito KATO; Hirofumi MIYAJI; Yuto YOSHINO; Yukimi KANEMOTO; Asako HAMAMOTO; Erika NISHIDA; Tsutomu SUGAYA; Saori TANAKA
  The Japanese Journal of Conservative Dentistry, 65, 2, 145, 153, The Japanese Society of Conservative Dentistry, 30 Apr. 2022, [Peer-reviewed], [Corresponding author]
  Japanese
• Osteoclast formation from mouse bone marrow cells on micro/nano-scale patterned surfaces.
  Tsukasa Akasaka; Hiroshi Hayashi; Miho Tamai; Yoshitaka Yoshimura; Yoh-Ichi Tagawa; Hirofumi Miyaji; Ko Nakanishi; Yasuhiro Yoshida
  Journal of oral biosciences, 64, 2, 237, 244, 07 Apr. 2022, [Peer-reviewed], [International Magazine]
  English, Scientific journal, OBJECTIVES: Osteoclasts can sense the surface topography of materials. However, it is difficult to identify the structural factors that affect osteoclast formation and its function. Furthermore, we hypothesized that the type of osteoclast precursor cells also affects osteoclastogenesis in the materials. In this study, we investigated the effects of defined micro/nanoscale patterns on osteoclastogenesis from bone marrow cells (BMCs). METHODS: Various cyclo-olefin polymer (COP) patterns were prepared using nanoimprinting. The effects of shape, size, and height of the patterns, and the wettability of the patterned surfaces on osteoclastogenesis from BMCs were evaluated in vitro. RESULTS: Osteoclast formation was promoted on pillars (diameter, 1 μm or 500 nm; height, 500 nm). Notably, osteoclastogenesis from BMCs was better promoted on hydrophobic pillars than on hydrophilic pillars. In contrast, decreased osteoclast formation was observed on the nanopillars (diameter, 100 nm; height, 200 nm). CONCLUSIONS: We demonstrated the promotion of osteoclast formation from BMCs on hydrophobic pillars with diameters of 1 μm and 500 nm. Some cellular behaviors in the patterns were dependent on the type of osteoclast precursor cells. The designed patterns are useful for designing the surface of dental implants or bone replacement materials with a controllable balance between osteoblast and osteoclast activities.
• 耐水性の高い酸化グラフェン/抗菌・抗ウイルス剤複合超薄膜コーティング
  宮治裕史; 郷田 隼; 小野博信
  MATERIAL STAGE, 22, 1, 44, 47, Apr. 2022, [Invited], [Lead author]
  Japanese
• Biological modification of tooth surface by laser-based apatite coating techniques.
  Hirofumi Miyaji; Ayako Oyane; Aiko Narazaki
  Journal of oral biosciences, 64, 2, 217, 221, 26 Mar. 2022, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, BACKGROUND: Development of new clinical regenerative procedures is needed for the reconstruction of the connective tissue attachment lost to periodontal disease. Apatite coating on the affected root surfaces could improve root surface biocompatibility and promote the reestablishment of connective tissue attachment. HIGHLIGHT: We developed two novel techniques that use laser light for coating the tooth surface with apatite. In the laser-assisted biomimetic (LAB) process, a tooth substrate was placed in a supersaturated calcium phosphate solution and irradiated for 30 minutes with low-energy pulsed laser light. Due to the laser-assisted pseudo-biomineralization, a submicron-thick apatite film was created on the laser-irradiated tooth surface. Furthermore, we created a fluoride-incorporated apatite film on the tooth surface using the LAB process and demonstrated its antibacterial activity against Streptococcus mutans. In the laser-induced forward transfer with optical stamp (LIFTOP) process, a thin apatite film loaded with the cell-adhesion protein, fibronectin, was prepared beforehand as a raw material on the optical stamp (carbon- and polydimethylsiloxane-coated support) by a conventional biomimetic process. After irradiation with a single laser pulse, the film (microchip) was transferred onto a tooth substrate via laser ablation of the carbon sacrificial layer. The LIFTOP process requires only a short processing time and has a minimal heat effect on the film; thus, the film exhibits cell adhesion activity even after the LIFTOP process. CONCLUSION: The LAB and LIFTOP processes have the potential as novel tools for tooth surface modification in the treatment of periodontal disease.

  
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• Ultrasonic irrigation of periodontal pocket with surface pre-reacted glass-ionomer (S-PRG) nanofiller dispersion improves periodontal parameters in beagle dogs
  Hirofumi Miyaji; Kayoko Mayumi; Yukimi Kanemoto; Ichie Okamoto; Asako Hamamoto; Akito Kato; Tsutomu Sugaya; Tsukasa Akasaka; Saori Tanaka
  Journal of Oral Biosciences, 64, 2, 222, 228, Elsevier {BV}, Mar. 2022, [Peer-reviewed], [Lead author, Corresponding author], [International Magazine]
  English, Scientific journal, OBJECTIVES: Surface pre-reacted glass-ionomer (S-PRG) nanofiller, an antibacterial ion-releasing bioactive glass, has been shown to adhere to tooth surfaces and reported to improve inflammatory parameters in experimental periodontitis. In this study, cementum substrate was irrigated ultrasonically with dispersion to examine in-vitro nanofiller adhesion and antibacterial activity. Moreover, periodontal pockets in a beagle dog were ultrasonically irrigated with dispersion to assess periodontal healing. METHODS: The morphology of human cementum irrigated with S-PRG nanofiller dispersion was examined by scanning electron microscopy and energy dispersive X-ray spectrometry. The antibacterial activity of the treated cementum was tested using Actinomyces naeslundii. In addition, experimentally formed periodontal pockets in beagle dog were ultrasonically irrigated with S-PRG nanofiller dispersion. Periodontal parameters (gingival index, bleeding on probing, probing pocket depth, and clinical attachment level) were measured from baseline (0 weeks) through 12 weeks. Moreover, the effects of irrigation with S-PRG nanofiller on changes in periodontal microflora and bone healing were analyzed. RESULTS: After ultrasonic irrigation, S-PRG nanofiller adhered to the cementum and exhibited antibacterial activity. The periodontal parameters were shown to improve following ultrasonic irrigation with S-PRG nanofiller dispersion. Analysis by next-generation sequencing revealed that the ratio of red-complex species decreased in the pockets irrigated with S-PRG nanofiller dispersion. In addition, the S-PRG nanofiller showed the potential to promote bone healing. CONCLUSIONS: Ultrasonic irrigation with S-PRG nanofiller dispersion using an ultrasonic scaler system permitted delivery of the S-PRG nanofiller to the root surface, providing improved parameters in experimental periodontitis and modifying the composition of subgingival periodontal microflora.

  
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• Different micro/nano-scale patterns of surface materials influence osteoclastogenesis and actin structure
  Tsukasa Akasaka; Miho Tamai; Yoshitaka Yoshimura; Natsumi Ushijima; Shinichiro Numamoto; Atsuro Yokoyama; Hirofumi Miyaji; Ryo Takata; Shuichi Yamagata; Yoshiaki Sato; Ko Nakanishi; Yasuhiro Yoshida
  Nano Research, 15, 5, 4201, 4211, 2022, [Peer-reviewed]
  Scientific journal

  
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• Periodontal tissue engineering using an apatite/collagen scaffold obtained by a plasma- and precursor-assisted biomimetic process.
  Yukimi Kanemoto; Hirofumi Miyaji; Erika Nishida; Saori Miyata; Kayoko Mayumi; Yuto Yoshino; Akihito Kato; Tsutomu Sugaya; Tsukasa Akasaka; Arputharaj Joseph Nathanael; Syama Santhakumar; Ayako Oyane
  Journal of periodontal research, 57, 1, 205, 218, 16 Nov. 2021, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, BACKGROUND AND OBJECTIVES: In the treatment of severe periodontal destruction, there is a strong demand for advanced scaffolds that can regenerate periodontal tissues with adequate quality and quantity. Recently, we developed a plasma- and precursor-assisted biomimetic process by which a porous collagen scaffold (CS) could be coated with low-crystalline apatite. The apatite-coated collagen scaffold (Ap-CS) promotes cellular ingrowth within the scaffold compared to CS in rat subcutaneous tissue. In the present study, the osteogenic activity of Ap-CS was characterized by cell culture and rat skull augmentation tests. In addition, the periodontal tissue reconstruction with Ap-CS in a beagle dog was compared to that with CS. METHODS: The plasma- and precursor-assisted biomimetic process was applied to CS to obtain Ap-CS with a low-crystalline apatite coating. The effects of apatite coating on the scaffold characteristics (i.e., surface morphology, water absorption, Ca release, protein adsorption, and enzymatic degradation resistance) were assessed. Cyto-compatibility and the osteogenic properties of Ap-CS and CS were assessed in vitro using preosteoblastic MC3T3-E1 cells. In addition, we performed in vivo studies to evaluate bone augmentation and periodontal tissue reconstruction with Ap-CS and CS in a rat skull and canine furcation lesion, respectively. RESULTS: As previously reported, the plasma- and precursor-assisted biomimetic process generated a low-crystalline apatite layer with a nanoporous structure that uniformly covered the Ap-CS surface. Ap-CS showed significantly higher water absorption, Ca release, lysozyme adsorption, and collagenase resistance than CS. Cell culture experiments revealed that Ap-CS was superior to CS in promoting the osteoblastic differentiation of MC3T3-E1 cells while suppressing their proliferation. Additionally, Ap-CS significantly promoted (compared to CS) the augmentation of the rat skull bone and showed the potential to regenerate alveolar bone in a dog furcation defect. CONCLUSION: Ap-CS fabricated by the plasma- and precursor-assisted biomimetic process provided superior promotion of osteogenic differentiation and bone neoformation compared to CS.
• Biomodification of tooth surface by laser-assisted apatite coating processes
  宮治裕史; 大矢根綾子; 奈良崎愛子
  New Glass, 36, 3, 15, 17, ニューガラスフォーラム, Nov. 2021, [Invited], [Lead author, Corresponding author]
  Japanese
• Bone Tissue Regeneration by Collagen Scaffolds with Different Calcium Phosphate Coatings: Amorphous Calcium Phosphate and Low-Crystalline Apatite.
  Syama Santhakumar; Ayako Oyane; Maki Nakamura; Yuto Yoshino; Mohammed Katib Alruwaili; Hirofumi Miyaji
  Materials (Basel, Switzerland), 14, 19, 07 Oct. 2021, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Surface-mineralized collagen sponges have attracted much attention as scaffolds for bone tissue engineering. Recently, we developed amorphous calcium phosphate (ACP) and low-crystalline apatite coating processes on collagen sponges. In the present study, we applied these coating processes to granular collagen sponges (referred to as Col) to compare the bone tissue regeneration capabilities of ACP-coated and apatite-coated Col (referred to as Col-ACP and Col-Ap, respectively) using a rat cranial bone defect model. According to micro-CT and histological analyses, Col-Ap enhanced bone tissue regeneration compared to Col, whereas Col-ACP did not. These results not only demonstrated the superior bone tissue regeneration capability of Col-Ap, but also indicated limitations of the in vitro simulated body fluid (SBF) test used in our previous study. Despite the apatite-forming ability of Col-ACP in SBF, it was ineffective in improving bone tissue regeneration in vivo, unlike Col-Ap, most likely due to the quick resorption of the ACP coating in the defect site. The present results clarified the importance of the coating stability in vivo and revealed that the low-crystalline apatite coating was more beneficial than the ACP coating in the fabrication of surface-mineralized collagen sponges for use as bone tissue engineering scaffolds.

  
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• Facile Fabrication Techniques of Antibacterial Calcium Phosphate Layers and Nanoparticles for Dental Applications
  大矢根綾子; 中村真紀; 宮治裕史; 宮治裕史
  Journal of the Society of Inorganic Materials, Japan, 28, 412, 115, 117, 無機マテリアル学会, May 2021, [Peer-reviewed], [Invited]
  Japanese
• Antibacterial and Antibiofilm Photodynamic Activities of Lysozyme-Au Nanoclusters/Rose Bengal Conjugates.
  Ichie Okamoto; Hirofumi Miyaji; Saori Miyata; Kanako Shitomi; Tsutomu Sugaya; Natsumi Ushijima; Tsukasa Akasaka; Satoshi Enya; Satoshi Saita; Hideya Kawasaki
  ACS omega, 6, 13, 9279, 9290, 06 Apr. 2021, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Antibacterial photodynamic therapy (aPDT) utilizes reactive oxygen species such as singlet oxygen (1O2) and free radicals via photosensitizers, which are light and light-sensitive agents, to reduce bacterial infections. It has been utilized as a treatment for dental diseases in place of antibiotic therapies. However, aPDT does not always cause the desired therapeutic effect due to the instability of organic photosensitizers and the formation of bacterial biofilms. To promote the antibacterial and antibiofilm effects of aPDT, we have proposed a lysozyme (Lys)-gold nanoclusters (Au NCs)/rose bengal (Lys-Au NCs/RB) conjugate as a novel photosensitizer. This conjugate was found to effectively impede the growth of both gram-positive and gram-negative bacteria when exposed to white light-emitting diode (LED) irradiation. The photoexcited Lys-Au NCs/RB showed significantly higher antibacterial activity than photoexcited Lys-Au NCs or RB alone. The synergistic effect is a result of the combination of Lys (an antibacterial protein) and enhanced 1O2 generation related to resonance energy transfer (RET) in the Au NCs/RB conjugate. Photoexcited Lys-Au NCs/RB increased the effects of aPDT in a dose- and time-dependent manner. Furthermore, the photoexcited Lys-Au NCs/RB successfully decreased Streptococcus mutans biofilm formation. However, in contrast, it did not have a negative effect on the proliferation, adhesion, or spread of mammalian cells, indicating low cytotoxicity. Lys-Au NCs/RB is a novel photosensitizer with low cytotoxicity that is capable of bacterial inactivation and the suppression of biofilm formation, and could help to improve dental treatments in the future.

  
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• Antibacterial coating of tooth surface with ion-releasing pre-reacted glass-ionomer (S-PRG) nanofillers.
  Kayoko Mayumi; Hirofumi Miyaji; Saori Miyata; Erika Nishida; Tomokazu Furihata; Yukimi Kanemoto; Tsutomu Sugaya; Kanako Shitomi; Tsukasa Akasaka
  Heliyon, 7, 2, e06147, Feb. 2021, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Objectives: Surface pre-reacted glass-ionomer (S-PRG) fillers release antibacterial borate and fluoride ions. We fabricated nanoscale S-PRG fillers (S-PRG nanofillers) for antibacterial coating of tooth surfaces and assessed the antibacterial effects of this coating in vitro. In addition, we creating a canine model of periodontitis to evaluate the effectiveness of S-PRG nanofiller application on tooth roots and improvement of periodontal parameters. Methods: Human dentin blocks were coated with S-PRG nanofiller (average particle size: 0.48 μm) and then characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectrometer (EDX), and ion-releasing test. Antibacterial effects of dentin blocks coated with S-PRG nanofiller were examined using bacterial strains, Streptococcus mutans and Actinomyces naeslundii. Next, we created an experimental model of periodontitis in furcation of premolars of beagle dogs. Then, S-PRG nanofiller coating was applied onto exposed tooth root surfaces. Periodontal parameters, gingival index (GI), bleeding on probing (BOP), probing pocket depth (PPD), and clinical attachment level (CAL), were measured from baseline until 4 weeks. In addition, bone healing was radiographically and histologically examined. Results: SEM and EDX revealed that S-PRG nanofillers uniformly covered the dentin surface after coating. Dentin blocks coated with S-PRG nanofiller showed ion-releasing property, bacterial growth inhibition, and sterilization effects. In the experimental periodontitis model, S-PRG nanofiller coating significantly reduced clinical inflammatory parameters, such as GI (P < 0.01) and BOP (P < 0.05), compared to uncoated samples. In addition, PPD and CAL significantly decreased by S-PRG nanofiller coating (2 weeks: P < 0.05; 3 and 4 weeks: P < 0.01), suggesting the improvement of periodontitis. Micro-CT and histology revealed that bone healing of furcation defects was enhanced by S-PRG nanofiller coating. Conclusion: S-PRG nanofiller coating provides antibacterial effects to tooth surfaces and improves clinical parameters of periodontitis.

  
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• Evaluation of antibacterial and cytocompatible properties of multiple-ion releasing zinc-fluoride glass nanoparticles.
  Erika Nishida; Hirofumi Miyaji; Kanako Shitomi; Tsutomu Sugaya; Tsukasa Akasaka
  Dental materials journal, 40, 1, 157, 164, 31 Jan. 2021, [Peer-reviewed], [Corresponding author], [Domestic magazines]
  English, Scientific journal, Zinc-fluoride glass nanoparticles (Zinc-F) release several ions, such as fluoride, zinc and calcium ions, through acid-base reactions. The aim of this study was to evaluate the antibacterial and cytotoxic properties of Zinc-F. Antibacterial tests showed that a Zinc-F eluting solution significantly reduced the turbidity and colony-forming units of Streptococcus mutans and Actinomyces naeslundii, compared to that of calcium-fluoroaluminosilicate glass nanoparticles without zinc ions. In live/dead staining, Zinc-F eluate significantly decreased green-stained bacterial cells, indicating live cells, compared with the control (no application). Human dentin coated with Zinc-F showed suppressed S. mutans and A. naeslundii biofilm formation. Additionally, Zinc-F eluate showed low cytotoxic effects in osteoblastic and fibroblastic cells. Therefore, our findings suggested that Zinc-F exhibits antibacterial and biocompatible properties through multiple-ion release.
• Laser-Induced Forward Transfer with Optical Stamp of a Protein-Immobilized Calcium Phosphate Film Prepared by Biomimetic Process to a Human Dentin
  Aiko Narazaki; Ayako Oyane; Hirofumi Miyaji
  Applied Sciences, 10, 22, 11 Nov. 2020, [Peer-reviewed], [Invited]
  English, Scientific journal

  
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• Antibacterial tooth surface created by laser-assisted pseudo-biomineralization in a supersaturated solution.
  Ayako Oyane; Ikuko Sakamaki; Kenji Koga; Maki Nakamura; Kanako Shitomi; Hirofumi Miyaji
  Materials science & engineering. C, Materials for biological applications, 116, 111170, 111170, Nov. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, A technique for implementing biocompatible and antibacterial functions to a targeted region on tooth surfaces has potential in dental treatments. We have recently demonstrated pseudo-biomineralization, i.e., the growth of an apatite layer on a human dentin substrate by a laser-assisted biomimetic (LAB) process, based on pulsed laser irradiation in a supersaturated CaP solution. In this study, pseudo-biomineralization was induced in the presence of fluoride ions using the LAB process in order to fabricate an antibacterial fluoride-incorporated apatite (FAp) layer on the dentin surface. After processing for 30 min, a micron-thick FAp layer was formed heterogeneously at the laser-irradiated solid-liquid interface via pseudo-biomineralization. A time-course study revealed that the LAB process first eliminated the pre-existing organic layer, while allowing fluoride incorporation into the dentin surface within 1 min. Within 5 min, FAp nanocrystals precipitated on the dentin surface. Within 30 min, these nanocrystals acquired a pillar-like structure that was weakly oriented in the direction normal to the substrate surface to form a dense micron-thick layer. This layer was integrated seamlessly with the underlying dentin without any apparent gaps. The FAp layer exhibited antibacterial activity against a major oral bacterium, Streptococcus mutans. The proposed LAB process is expected to be a useful new tool for tooth surface functionalization via facile and area-specific pseudo-biomineralization.
• In situ precipitation of amorphous calcium phosphate nanoparticles within 3D porous collagen sponges for bone tissue engineering.
  Syama Santhakumar; Ayako Oyane; Maki Nakamura; Kenji Koga; Saori Miyata; Ko Muratsubaki; Hirofumi Miyaji
  Materials science & engineering. C, Materials for biological applications, 116, 111194, 111194, Nov. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Amorphous calcium phosphate (ACP) plays an important role in biomineralization within the three-dimensional (3D) collagen network in human hard tissues, and exhibits osteoconductivity. Porous collagen sponges coated with ACP nanoparticles could be considered as potential scaffolds for use in bone tissue engineering. In this study, such composite materials were fabricated via homogeneous ACP precipitation using a supersaturated calcium phosphate (CaP) solution. Homogeneous ACP precipitation was induced in situ within the sponges by a temperature-controlled coating process composed of two steps. In the first step, the CaP solution was cooled to 4 °C to suppress precipitation until the solution penetrated fully into the sponge's internal pores. In the second step, the CaP solution was warmed up to 25 °C with continuous shaking to induce ACP precipitation within the sponges. The resulting sponges were therefore coated with ACP nanoparticles on their inner and outer surfaces. A simulated body fluid (SBF) test indicated osteoconductivity of the collagen sponges coated with ACP nanoparticles. Further, ACP-coated collagen sponges immobilizing basic fibroblast growth factor (bFGF) were fabricated using the CaP solution supplemented with bFGF. The fabricated sponges allowed the sustained release of bFGF in a culture medium and enhanced proliferation of osteoblastic MC3T3-E1 cells. Such ACP-coated collagen sponges have the potential to be used as scaffolds in bone tissue engineering if pursued for further in vitro and in vivo studies.
• Technique for simple apatite coating on a dental resin composite with light-curing through a micro-rough apatite layer.
  Yasushi Hibino; Ayako Oyane; Kanako Shitomi; Hirofumi Miyaji
  Materials science & engineering. C, Materials for biological applications, 116, 111146, 111146, Nov. 2020, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Tooth root surfaces restored with dental resin composites exhibit inferior biocompatibility. The objective of this study was to develop a simple technique for coating apatite onto a resin composite to improve its surface biocompatibility. First, we fabricated a polymer film coated with a micro-rough apatite layer and pressed it (coating-side down) onto a viscous resin composite precursor. As a result of light-induced curing of the precursor through the overlaid film, the micro-rough apatite layer was integrated with the resin composite and, thus, transferred from the polymer film surface to the cured resin composite surface as a result of the difference in interfacial adhesion strength. The transferred apatite layer attached directly to the cured resin composite without any gaps at the microscopic level. The adhesion between the apatite layer and the cured resin composite was so strong that the layer was not peeled off even by a tape-detaching test. The flexural strength of the resulting apatite-coated resin composite was comparable to that of the clinically used resin composite while satisfying the ISO requirement for dental polymer-based restorative materials. Furthermore, the apatite-coated resin composite showed better cell compatibility than the uncoated resin composite. The present apatite coating technique is well suited for dental treatment because the coating is applied during a conventional light curing procedure through simple utilization of the apatite-coated polymer film in place of an uncoated film. The proposed technique represents a practical evolution in dental treatment using light-curing resin composites, although further in vitro and in vivo studies are needed.
• Bone forming ability of recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles.
  Tomokazu Furihata; Hirofumi Miyaji; Erika Nishida; Akihito Kato; Saori Miyata; Kanako Shitomi; Kayoko Mayumi; Yukimi Kanemoto; Tsutomu Sugaya; Tsukasa Akasaka
  Journal of biomedical materials research. Part B, Applied biomaterials, 108, 7, 3033, 3044, Oct. 2020, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal
• クリニカル・アドバンス，2ペーストタイプ根管充填シーラー「キャナルスペースト」の抗菌性，細胞親和性評価．
  宮治裕史
  デンタルダイヤモンド, 45, 168, 170, Aug. 2020, [Invited], [Lead author]
• Suppression of root caries progression by application of Nanoseal®: A single-blind randomized clinical trial.
  Hirofumi Miyaji; Akihito Kato; Saori Tanaka
  Dental materials journal, 39, 3, 444, 448, 05 Jun. 2020, [Peer-reviewed], [Lead author, Corresponding author], [Domestic magazines]
  English, Scientific journal, The aim of this single-blind, parallel and randomized controlled trial was to evaluate the effect of Nanoseal® application on root caries progression. Adult patients (n=129, mean age: 66.4±10.1 years) with root caries were randomly allocated into three groups: high-frequency (HF, n=43; intervention: Nanoseal application at baseline and 1-5 months), low-frequency (LF, n=43; intervention: Nanoseal application at baseline and 3 months), and control (n=43; intervention: no application of Nanoseal) groups. Measurements of fluorescence laser values of carious lesions using a DIAGNOdent™ Pen (D-value) were performed for each subject before intervention (baseline) and at 3 and 6 months. Significantly lower D-values for the HF (p=0.017) and LF (p=0.034) groups were observed compared with the control group at 6 months. Nanoseal application would be an effective procedure to suppress root caries progression.
• Photodynamic inactivation of oral bacteria with silver nanoclusters/rose bengal nanocomposite.
  Kanako Shitomi; Hirofumi Miyaji; Saori Miyata; Tsutomu Sugaya; Natsumi Ushijima; Tsukasa Akasaka; Hideya Kawasaki
  Photodiagnosis and photodynamic therapy, 30, 101647, 101647, Jun. 2020, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Antimicrobial photodynamic therapy (a-PDT) is a promising anti-infective technique for generation of singlet oxygen (1O2) to target dental disease. However, conventional organic photosensitizers have problems for clinical use in terms of cytotoxicity, quenching of a-PDT activity by self-dimerization, and the lack of long-term antibacterial effect. We herein propose silver nanoclusters/rose bengal nanocomposite (AgNCs/RB) as a novel photosensitizer with two primary antibacterial effects: (1) 1O2 generation by irradiated RB and (2) Ag+ ion release from AgNCs. AgNCs/RB irradiated with white light-emitting diode (LED) for a short irradiation time of 1 min significantly decreased the bacterial turbidity of Streptococcus mutans, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans (P < 0.05). In SEM, TEM and LIVE/DEAD staining images, photoexcited AgNCs/RB reduced S. mutans colonization, destroyed the cell membrane, and increased the number of dead cells. The antibacterial efficiency of photoexcited AgNCs/RB was greater than that of AgNCs or RB alone (P < 0.05), suggesting a synergistic effect of 1O2 and Ag+ ions from photoexcited AgNCs/RB. By contrast, photoexcited AgNCs/RB did not affect WST-8 and LDH activities and morphology of NIH3T3 mammalian cells, indicating low cytotoxicity. Interestingly, the antibacterial activity of AgNCs/RB on S. mutans was maintained even after the cessation of LED irradiation, indicating a long-term antibacterial effect due to released Ag+ ions. The present AgNCs/RB photosensitizers provide effective synergistic antibacterial effects for dental a-PDT via 1O2 and Ag+ ions coupled with low cytotoxicity.
• Comparative biological assessments of endodontic root canal sealer containing surface pre-reacted glass-ionomer (S-PRG) filler or silica filler.
  Hirofumi Miyaji; Kayoko Mayumi; Saori Miyata; Erika Nishida; Kanako Shitomi; Asako Hamamoto; Saori Tanaka; Tsukasa Akasaka
  Dental materials journal, 39, 2, 287, 294, 31 Mar. 2020, [Peer-reviewed], [Lead author, Corresponding author], [Domestic magazines]
  English, Scientific journal, Surface pre-reacted glass-ionomer (S-PRG) filler releases several ions, such as fluoride, borate and strontium ions, to exert bioactive effects. We fabricated an endodontic root canal sealer containing S-PRG fillers (S-PRG sealer) and then evaluated the antibacterial and anti-inflammatory properties of S-PRG sealer compared with sealer containing conventional silica fillers (silica sealer). Antibacterial tests showed that S-PRG sealer significantly reduced the turbidity of Enterococcus faecalis compared with silica sealer. Implantation of S-PRG or silica sealer blocks in rat subcutaneous tissue showed that S-PRG sealer decreased the proinflammatory response compared with silica sealer at 10 days post-implantation. In addition, immunostaining revealed that infiltration of CD68- and peroxidase-positive cells around the S-PRG sealer was significantly lower than that in silica sealer. Therefore, it was suggested that S-PRG sealer exhibits antibacterial and anti-inflammatory effects.
• Laser-assisted biomineralization on human dentin for tooth surface functionalization.
  Ayako Oyane; Noriyuki Saito; Ikuko Sakamaki; Kenji Koga; Maki Nakamura; A Joseph Nathanael; Noriko Yoshizawa; Kanako Shitomi; Kayoko Mayumi; Hirofumi Miyaji
  Materials science & engineering. C, Materials for biological applications, 105, 110061, 110061, Dec. 2019, [Peer-reviewed], [International Magazine]
  English, Scientific journal, A technique for tooth surface modification with biocompatible calcium phosphate (CaP) has huge potential in dental applications. Recently, we achieved a facile and area-specific CaP coating on artificial materials by a laser-assisted biomimetic process (LAB process), which consists of pulsed laser irradiation in a supersaturated CaP solution. In this study, we induced the rapid biomineralization on the surface of human dentin by using the LAB process. A human dentin substrate was immersed in a supersaturated CaP solution, then its surface was irradiated with weak pulsed laser light for 30 min (LAB process). Ultrastructural analyses revealed that the pristine substrate had a demineralized collagenous layer on its surface due to the previous EDTA surface cleaning. After the LAB process, this collagenous layer disappeared and was replaced with a submicron-thick hydroxyapatite layer. We believe that the laser irradiation induced pseudo-biomineralization through the laser ablation of the collagenous layer, followed by CaP nucleation and growth at the dentin-liquid interface. The mineralized layer on the dentin substrate consisted of needle-like hydroxyapatite nanocrystals, whose c-axes were weakly oriented along the direction perpendicular to the substrate surface. This LAB process would offer a new tool enabling tooth surface modification and functionalization through the in situ pseudo-biomineralization.
• Bioactive micropatterning of apatite immobilizing cell adhesion protein by laser-induced forward transfer with a shock absorber
  Narazaki Aiko; Oyane Ayako; Komuro Saki; Kurosaki Ryozo; Kameyama Tomoko; Sakamaki Ikuko; Araki Hiroko; Miyaji Hirofumi
  OPTICAL MATERIALS EXPRESS, 9, 7, 2807, 2816, 01 Jul. 2019, [Peer-reviewed]
  English, Scientific journal

  
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• 銀ナノクラスターで被覆したヒト象牙質はStreptococcus mutansに対する抗菌活性を示す(Human Dentin Coated with Silver Nanoclusters Exhibits Antibacterial Activity against Streptococcus mutans)
  Shitomi Kanako; Miyaji Hirofumi; Miyata Saori; Nishida Erika; Mayumi Kayoko; Sugaya Tsutomu; Kawasaki Hideya
  Nano Biomedicine, 11, 1, 21, 28, ナノ・バイオメディカル学会, Jun. 2019, [Peer-reviewed], [Corresponding author]
  English, 虫歯の病原菌であるStreptococcus mutansに対して審美的(象牙質変色予防)作用と抗菌的作用を持つ成分として、直径2nm未満の銀ナノクラスター(AgNC)を調製した。このAgNCはS.mutansの濁度と生存性を顕著に減少させるだけでなく、LIVE/DEAD染色法にて殺菌効果が認められた。このAgNCをヒト象牙質に適用すると、フッ化ジアミン銀を適用した場合と比べて象牙質の変色は認められず、AgNC-処理象牙質はS.mutansによるコロニー形成に対する阻害効果を示した。これらの結果から、AgNCは抗菌的・審美的基質として、歯科療法で有望と考えられた。, 11819377

  
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• Assessments of Biocompatibility of Root Canal Sealer Containing S-PRG Filler
  Saori TANAKA; Hirofumi MIYAJI; Erika NISHIDA; Saori MIYATA; Akihito KATO; Kayoko MAYUMI; Toru TANAKA; Satoshi INOUE
  The Japanese Journal of Conservative Dentistry, 62, 2, 107, 114, 特定非営利活動法人 日本歯科保存学会, Apr. 2019, [Peer-reviewed]
  Japanese, <p> Purpose: Surface pre-reacted glass-ionomer (S-PRG) fillers reportedly possess ion-releasing properties and exhibit bioactive effects. The purpose of this study was to evaluate the biocompatibility of a zinc oxide based sealer containing S-PRG fillers (S-PRG sealer) compared with zinc oxide eugenol (ZOE) and non eugenol (ZO) sealers.</p><p> Method: MC3T3-E1 cells were seeded on disks of S-PRG, ZOE and ZO sealers. Observations by scanning electron microscopy (SEM) and cell viability assay were carried out. Subsequently, each specimen was implanted into dorsal subcutaneous tissue of rats. After 10 and 35 days, the degree of inflammatory cell infiltration was evaluated by light microscopy using histological samples.</p><p> Results: SEM observation revealed cell proliferation on S-PRG sealer, but not on ZOE and ZO sealers. Cell viability on S-PRG sealer was significantly greater than that on ZOE and ZO sealers. At 10 days after implantation into rat subcutaneous tissue, all samples showed inflammatory cell infiltration, however, S-PRG sealer tended to cause a milder inflammatory response than ZOE and ZO sealers. Ten-day inflammation scores of S-PRG, ZOE and ZO sealers were 2.16, 2.67 and 2.83, respectively. At 35 days after implantation, inflammatory cells were rarely found around the S-PRG sealer. Inflammation scores of S-PRG, ZOE and ZO sealers at 35 days were 0.00, 0.67 and 0.67, respectively.</p><p> Conclusion: S-PRG sealer possesses superior biocompatibility compared to ZOE and ZO sealers.</p>
• 重度歯周炎と精神遅滞を伴う成人上顎前突症例
  菅原 由紀; 宮治 裕史; 佐藤 嘉晃
  北海道歯学雑誌, 39, 2, 137, 145, 北海道歯学会, Mar. 2019, [Peer-reviewed]
  Japanese

  
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• クリニカル・アドバンス 抗菌性イオンが口腔ケアをサポートするBioUnionテクノロジー!!
  宮治 裕史
  DENTAL DIAMOND, 44, 3, 194, 199, (株)デンタルダイヤモンド社, Feb. 2019, [Invited], [Lead author]
  Japanese
• Laser additive micro-patterning for biomedical applications using laser-induced transfer
  奈良崎 愛子; 大矢根 綾子; 宮治 裕史
  ICALEO 2019 Proceedings, -, 2019
  Scientific journal
• 医療・ヘルスケアを支える高機能材料 研究開発事例 高度歯科医療のためのリン酸カルシウム薄膜・ナノ粒子の迅速液相合成
  大矢根綾子; 中村真紀; 宮治裕史
  工業材料, 67, 10, 59‐63, 63, 2019
  Japanese
• Fusion of RAW 264.7 macrophage cells on micro-scale fine pillar patterns
  J Honma; T Akasaka; M Tamai; Y Yoshimura; T Taira; H Miyaji; S Yamagata; Y Sato; Y Yoshida
  Dig J Nanomater Bios, 13, 4, 1123, 1131, Dec. 2018, [Peer-reviewed]
  English, Scientific journal

  
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• Rapid and area-specific coating of fluoride-incorporated apatite layers by a laser-assisted biomimetic process for tooth surface functionalization.
  A Joseph Nathanael; Ayako Oyane; Maki Nakamura; Moumita Mahanti; Kenji Koga; Kanako Shitomi; Hirofumi Miyaji
  Acta biomaterialia, 79, 148, 157, 01 Oct. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Surface functionalization of teeth with fluoride-incorporated apatite layers displays great potential in treatments and prevention of dental disorders. In this study, we used a sintered hydroxyapatite (sHA) substrate as a model material of teeth, and established a rapid and area-specific coating technique of fluoride-incorporated apatite layers by using a laser-assisted biomimetic (LAB) process. In this technique, a sHA substrate was irradiated on the surface with a Nd:YAG pulsed UV laser for 30 min in supersaturated calcium phosphate (CaP) solutions with various fluoride concentrations. The fluoride concentration in the CaP solution was varied to control morphology, crystalline structure, and fluoride content of the resulting layers. Without fluoride in the CaP solution, an octacalcium phosphate (OCP) layer with a flake-like structure was formed on the laser-irradiated surface of the substrate. The addition of fluoride (1000 µM and 3000 µM) to the CaP solution led to the formation of fluoride-incorporated apatite layers with an enamel-like needle-like nanostructure. The fluoride-incorporated apatite layers adhered firmly to the sHA surface and reduced acid dissolution of the sHA substrate by acting as a protective covering. Additionally, the layers released fluoride ions for more than 24 h, and exhibited antibacterial activity relative to a caries-causing bacterium, namely Streptococcus mutans. Thus, our LAB process can potentially act as a new tool for functionalization of tooth surfaces. STATEMENT OF SIGNIFICANCE: We used a sintered hydroxyapatite (sHA) substrate as a model material of teeth, and established a rapid and area-specific coating technique of fluoride-incorporated apatite layers on the sHA surface by using our laser-assisted biomimetic (LAB) process. In this process, pulsed laser was utilized to accelerate seeded crystal growth in supersaturated calcium phosphate solutions supplemented with NaF. The thus-fabricated fluoride-incorporated apatite layers consisted of enamel-like needle-like nanocrystals with c-axis orientation. These fluoride-incorporated apatite layers adhered firmly to the sHA surface, reduced acid dissolution of the sHA substrate by acting as a protective covering, and exhibited antibacterial activity against Streptococcus mutans through the fluoride release. Thus, our LAB process can potentially act as a new tool for functionalization of tooth surfaces.
• Antibacterial Photocurable Acrylic Resin Coating Using a Conjugate between Silver Nanoclusters and Alkyl Quaternary Ammonium
  Chiaki Tominaga; Kanako Shitomi; Hirofumi Miyaji; Hideya Kawasaki
  ACS APPLIED NANO MATERIALS, 1, 9, 4809, 4818, Sep. 2018, [Peer-reviewed]
  English, Scientific journal
• Calcium phosphate coating on dental composite resins by a laser-assisted biomimetic process.
  A Joseph Nathanael; Ayako Oyane; Maki Nakamura; Kenji Koga; Erika Nishida; Saori Tanaka; Hirofumi Miyaji
  Heliyon, 4, 8, e00734, Aug. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Objectives: Dental composite resins with better biocompatibility and osteoconductivity have been sought in endodontic treatments. This study aimed to develop a technique to produce the osteoconductive resin surfaces through calcium phosphate (CaP) coating using a laser-assisted biomimetic (LAB) process. Methods: Light-cured, acrylic-based composite resins were used as substrates. The resin substrate was subjected to a LAB process comprising Nd:YAG pulsed laser irradiation in a supersaturated CaP solution. The LAB-processed substrate was immersed for 3 days in a simulated body fluid (SBF) for the preliminary osteoconductivity assessment. Results: After irradiation for 30 min, the resin surfaces were partly coated with a newly formed CaP layer. The coating layer contained hydroxyapatite as the main crystalline phase and the coating coverage depended on the laser wavelength and the type of resin. The LAB-processed CaP-coated surface exhibited apatite-forming ability in SBF. Conclusions: LAB process is effective for CaP coating on light-cured dental composite resins and improving their osteoconductivity. Clinical significance: The LAB process is a potential new tool to create a cementum-like osteoconductive surface on dental composite resins.

  
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• Aggregation/Self-Assembly-Induced Approach for Efficient AuAg Bimetallic Nanocluster-Based Photosensitizers
  Daiki Hikosou; Sastoshi Saita; Saori Miyata; Hirofumi Miyaji; Tetsuya Furuike; Hiroshi Tamura; Hideya Kawasaki
  Journal of Physical Chemistry C, 122, 23, 12494, 12501, 14 Jun. 2018, [Peer-reviewed]
  English, Scientific journal
• Effect of a nano-scale fine hole pattern on the differentiation of RAW264.7 cells into osteoclasts
  R Takata; T Akasaka; M Tamai; Y Yoshimura; T Taira; H Miyaji; Y Tagawa; S Yamagata; J Iida; Y Yoshida
  Dig J Nanomater Bios, 13, 2, 451, 458, Apr. 2018, [Peer-reviewed]
  English, Scientific journal

  
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• 安全かつ革新的な骨疾患治療を可能にする高分子医薬の創出
  岩崎泰彦; 宮治裕史; 平賀徹
  技苑, 146, 219‐221, 31 Mar. 2018
  Japanese
• Evaluation of tissue behavior on three-dimensional collagen scaffold coated with carbon nanotubes and β-tricalcium phosphate nanoparticles
  H Miyaji; S Murakami; E Nishida; T Akasaka; B Fugetsu; J Umeda; K Kondoh; T Iizuka; T Sugaya
  J Oral Tissue Engin, 15, 3, 123, 130, Mar. 2018, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal

  
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• Bovine serum albumin-capped gold nanoclusters conjugating with methylene blue for efficient 1O2 generation via energy transfer.
  Masaki Yamamoto; Kanako Shitomi; Saori Miyata; Hirofumi Miyaji; Hiroyuki Aota; Hideya Kawasaki
  Journal of colloid and interface science, 510, 221, 227, 15 Jan. 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal
• Near-infrared Irradiation and Graphene Oxide Film Fabricated on Dentin Surface Exhibit Photothermal and Antibacterial Effects
  Nagao Keishi; Miyaji Hirofumi; Nishida Erika; Akasaka Tsukasa; Miyata Saori; Shitomi Kanako; Mayumi Kayoko; Kato Akihito; Sugaya Tsutomu
  Journal of Oral Hygiene & Health, 6, 1, 231, 231, OMICS International, 10 Jan. 2018, [Peer-reviewed], [Corresponding author]
  English, Scientific journal

  
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• Laser Lift-Off Process for Additive Micropatterning of Functional Particles and Films
  Aiko Narazaki; Tadatake Sato; Hiroyuki Niino; Yoshiki Nakata; Tatsuya Shoji; Yasuyuki Tsuboi; Ayako Oyane; Hirofumi Miyaji
  2018 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS), 1, 6, 2018
  English, International conference proceedings
• Preparation of micro/nanopatterned gelatins crosslinked with genipin for biocompatible dental implants.
  Reika Makita; Tsukasa Akasaka; Seiichi Tamagawa; Yasuhiro Yoshida; Saori Miyata; Hirofumi Miyaji; Tsutomu Sugaya
  Beilstein journal of nanotechnology, 9, 1735, 1754, 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Background: Collagen is a basic component of the periodontium and plays an important role in the function of the periodontal unit. Therefore, coating with collagen/gelatin has been applied to enable dental implants to positively interact with peri-implant tissues. Although the micro/nanoscale topography is an important property of the surface of dental implants, smaller collagen/gelatin surface patterns have not been sufficiently developed. Furthermore, only few reports on the behavior of cells on gelatin surfaces with different patterns and sizes exist. In this study, we developed micro/nanometer-scaled gelatin surfaces using genipin crosslinking, with the aim of understanding the use of patterning in surface modification of dental implants. Results: Grooves, holes, and pillars, with widths or diameters of 2 µm, 1 µm, or 500 nm were fabricated using a combination of molding and genipin crosslinking of gelatin. The stability of the different gelatin patterns could be controlled by the degree of genipin crosslinking. The gelatin patterns at 20 mM concentration of genipin and 41% crosslinking maintained a stable, patterned shape for at least 14 days in a cell culture medium. A cell morphology study showed that the cells on groves were aligned along the direction of the grooves. In contrast, the cells on pillars and holes exhibited randomly elongated filopodia. The vinculin spots of the cells were observed on the top of ridges and pillars or the upper surface of holes. The results of a cell attachment assay showed that the number of surface-attached cells increased with increasing patterning of the gelatin surface. Unlike the cell attachment assay, the results of a cell proliferation assay showed that Saos-2 cells prefer grooves with diameters of approximately 2 µm and 1 µm and pillars with diameters of 1 µm and heights of 500 nm. The number of cells on pillars with heights of 2 µm was larger than those of the other gelatin surface patterns tested. Conclusion: These data support that a detailed design of the gelatin surface pattern can control both cell attachment and proliferation of Saos-2 cells. Thus, gelatin surfaces patterned using genipin crosslinking are now an available option for biocompatible material patterning.
• Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog.
  Kohei Kawamoto; Hirofumi Miyaji; Erika Nishida; Saori Miyata; Akihito Kato; Akito Tateyama; Tomokazu Furihata; Kanako Shitomi; Toshihiko Iwanaga; Tsutomu Sugaya
  International journal of nanomedicine, 13, 2365, 2376, 2018, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal

  
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• Laser-assisted wet coating of calcium phosphate for surface-functionalization of PEEK.
  Ayako Oyane; Maki Nakamura; Ikuko Sakamaki; Yoshiki Shimizu; Saori Miyata; Hirofumi Miyaji
  PloS one, 13, 10, e0206524, 2018, [Peer-reviewed], [International Magazine]
  English, Scientific journal, Calcium phosphate (CaP) coating is an effective method for surface-functionalization of bioinert materials and for production of osteoconductive implants. Recently, we developed a laser-assisted biomimetic process (LAB process) for facile and area-specific CaP coating. In this study, the LAB process was applied to chemically stable and mechanically durable poly(etheretherketone) (PEEK), which has become widely used as an orthopedic and dental implant material. The LAB process was carried out by irradiating pulsed Nd:YAG laser light (355 nm) onto a PEEK substrate that was immersed in supersaturated CaP solution. The CaP coating applicability depended on laser fluence, i.e., CaP successfully formed on PEEK surface after the LAB process at 2 W/cm2. Further increase in laser fluence did not result in the successful formation. At the optimal fluence of 2 W/cm2, the laser-irradiated PEEK surface was modified and heated to induce heterogeneous CaP precipitation within 10 min in CaP solution, followed by further CaP growth over the irradiation time (tested up to 30 min). The LAB process improved the cytocompatibility of PEEK surface with osteoblastic MC3T3-E1 cells. Furthermore, the LAB-processed CaP-coated PEEK substrate formed a dense hydroxyapatite layer on its surface in the simulated body fluid, suggesting the osteoconductivity of this material. The present LAB process can be a useful new tool to produce osteoconductive PEEK-based implants.

  
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• シンバスタチン含有α型リン酸トリカルシウムミクロ粒子懸濁液による骨誘導(Bone Induction by α-tricalcium Phosphate Microparticle Emulsion Containing Simvastatin)
  Tateyama Akito; Kato Akihito; Miyaji Hirofumi; Nishida Erika; Iwasaki Yasuhiko; Fujii Syuji; Kawamoto Kohei; Shitomi Kanako; Furihata Tomokazu; Mayumi Kayoko; Sugaya Tsutomu
  Nano Biomedicine, 9, 2, 69, 76, ナノ・バイオメディカル学会, Dec. 2017, [Peer-reviewed], [Corresponding author]
  English, Scientific journal, 骨移植材の分解性と操作性を改善するため、α型リン酸トリカルシウム(α-TCP)骨ペーストを基にした水-オイル懸濁液を調製した。親油性の抗高脂血症薬であるシンバスタチン(SIM)には骨形成促進作用が認められているため、SIM含有α-TCP骨ペーストを用いてラットの頭蓋骨欠損に対する骨形成効果を評価した。骨ペーストは固化後に多孔性構造とヒドロキシアパタイトの生成を示すので、頭蓋骨欠損部への移植後にX線画像解析と組織学的検査を行った。骨ペーストのSIM含有量は0、0.01、0.1および1mgとし、無SIMの対照骨ペーストと比較した。その結果、骨ペースト移植後に新たな骨形成が認められ、特に0.1mg SIM群では対照群に比べて骨体積が有意に促進され、術後4週では骨欠損部に骨ペースト残渣は見られなかった。以上より、SIM含有α-TCP骨ペーストは分解性であり、骨組織工学にとって有用であることが示唆された。

  
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• Dose effects of beta-tricalcium phosphate nanoparticles on biocompatibility and bone conductive ability of three-dimensional collagen scaffolds.
  Shusuke Murakami; Hirofumi Miyaji; Erika Nishida; Kohei Kawamoto; Saori Miyata; Hiroko Takita; Tsukasa Akasaka; Bunshi Fugetsu; Toshihiko Iwanaga; Hiromi Hongo; Norio Amizuka; Tsutomu Sugaya; Masamitsu Kawanami
  Dental materials journal, 36, 5, 573, 583, 26 Sep. 2017, [Peer-reviewed], [Corresponding author], [Domestic magazines]
  English, Scientific journal, Three-dimensional collagen scaffolds coated with beta-tricalcium phosphate (β-TCP) nanoparticles reportedly exhibit good bioactivity and biodegradability. Dose effects of β-TCP nanoparticles on biocompatibility and bone forming ability were then examined. Collagen scaffold was applied with 1, 5, 10, and 25 wt% β-TCP nanoparticle dispersion and designated TCP1, TCP5, TCP10, and TCP25, respectively. Compressive strength, calcium ion release and enzyme resistance of scaffolds with β-TCP nanoparticles applied increased with β-TCP dose. TCP5 showed excellent cell-ingrowth behavior in rat subcutaneous tissue. When TCP10 was applied, osteoblastic cell proliferation and rat cranial bone augmentation were greater than for any other scaffold. The bone area of TCP10 was 7.7-fold greater than that of non-treated scaffold. In contrast, TCP25 consistently exhibited adverse biological effects. These results suggest that the application dose of β-TCP nanoparticles affects the scaffold bioproperties; consequently, the bone conductive ability of TCP10 was remarkable.
• Treatment of infected root canals with 4-META/MMA-TBB resin
  T Sugaya; S Tanaka; H Miyaji; S Shimoji; M Kudo; H Takefu; T Washizu
  Hokkaido J Dent Sci, 38, 111, 121, 北海道歯学会, Sep. 2017, [Peer-reviewed]
  English, This review paper describes the various applications of 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate-tri-n-butyl borane (4-META/MMA-TBB) and reviews research studies on the treatment of infected root canals using 4-META/MMA-TBB resin. 4-META/MMA-TBB resin exhibits good biocompatibility, polymerisation, and adhesiveness to dentine. As a result, it has improved the previously low success rates of root-end sealing, root canal filling, and perforation sealing in difficult cases. Root-end sealing of resected surfaces using 4-META/MMA-TBB during apicoectomy and intentional replantation prevents leakage from root canal and root resorption. 4-META/MMA-TBB can offer sealing for root canals with opened root apex in which pressure could not be applied during root canal filling. In this paper we will discuss clinical cases related to the application of this resin and the benefits of 4-META/MMA-TBB resin.
• Antibacterial and cytotoxic effects of photoexcited Au clusters via blue high-power or white low-power light-emitting diode irradiation
  S Miyata; H Miyaji; H Kawasaki; E Nishida; K Shitomi; T Akasaka; S Tanaka; T Iizuka; T Sugaya
  Biol Eng Med, Sep. 2017, [Peer-reviewed], [Corresponding author]
  English

  
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• In Vitro and in Vivo Analysis of Mineralized Collagen-Based Sponges Prepared by a Plasma- and Precursor-Assisted Biomimetic Process.
  A Joseph Nathanael; Ayako Oyane; Maki Nakamura; Ikuko Sakamaki; Erika Nishida; Yukimi Kanemoto; Hirofumi Miyaji
  ACS applied materials & interfaces, 9, 27, 22185, 22194, 12 Jul. 2017, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, Three-dimensional (3D) porous scaffolds for supporting cell adhesion and growth play a vital role in tissue engineering applications. In the present study, three different collagen-based 3D sponges were functionalized by apatite coating. The sponges were coated with apatite on their outer and inner surfaces while retaining their interconnecting pores. To achieve this, we employed a vacuum degassing system in our plasma- and precursor-assisted biomimetic process using a supersaturated calcium phosphate solution. The resulting apatite-coated sponges (mineralized sponges) showed better cell adhesion properties in vitro for osteoblast-like MC3T3-E1 cells compared to that of uncoated sponges. The three mineralized sponges were implanted in the subcutaneous tissue of rats. Upon histological evaluation after 10 days, the mineralized sponges showed cell in-growth rates that were approximately 4-fold greater than those of the untreated sponges without any notable inflammatory reactions. As these sponges are composed of clinically approved collagen-based frameworks and possess a 3D porous structure with a mineralized surface appropriate for cell adhesion and internalization, further in vitro and in vivo studies should be conducted regarding tissue engineering applications.
• SUBMICRO-PATTERNING OF CURABLE DENTAL MATERIALS BY MOLDING METHODS: A SCREENING TRIAL
  T. Akasaka; H. Miyaji; T. Imamura; N. Kaga; A. Yokoyama; Y. Yoshida
  DIGEST JOURNAL OF NANOMATERIALS AND BIOSTRUCTURES, 12, 2, 281, 292, Apr. 2017, [Peer-reviewed]
  English, Scientific journal
• Periodontal tissue repair after sealing of the gap in vertical root fracture.
  Tsutomu Sugaya; Mahito Tomita; Youji Motoki; Khurshiduz Zaman; Hirofumi Miyaji; Masamitsu Kawanami
  Odontology, 105, 2, 202, 207, Apr. 2017, [Peer-reviewed], [Domestic magazines]
  English, Scientific journal, The aim of this study was to determine whether sealing of fracture gap using adhesive resin through the root canal can prevent inflammation of periodontal tissue, and resealing the incompletely sealed fracture gap from outside can resolve such inflammation in experimentally created vertical root fractures. Vertical root fractures were created in incisor of beagles. In the experimental group, the fracture gap was sealed through the root canal with adhesive resin. After 5 weeks, sites with the clinical attachment level ≥4 mm were further divided randomly into the poor-replanting group and the poor-untreated group. In the poor-replanting group, the tooth was extracted and replanted after resealing the fracture gap with adhesive resin from the outer surface. Sites with clinical attachment level ≤3 mm after 5 weeks were considered as the satisfactory group. The poor-untreated group and the satisfactory group were subjected to no further treatment. The clinical attachment level was evaluated at baseline and after 2, 5, and 9 weeks. After 9 weeks, histological measurements were made to determine the length of the epithelial downgrowth and the area of alveolar bone resorption. The clinical attachment level and the area of bone resorption were significantly smaller in the poor-replanting group and the satisfactory group than in the poor-untreated group (p < 0.05). The results indicate the possibility that periodontal inflammation along the fracture line can be prevented and improved if the fracture gap is sealed.
• 安全かつ革新的な骨疾患治療を可能にする高分子医薬の創出
  岩崎泰彦; 宮治裕史
  技苑, 144, 221‐223, 28 Mar. 2017
  Japanese
• Antimicrobial photodynamic activity and cytocompatibility of Au25(Capt)18 clusters photoexcited by blue LED light irradiation.
  Saori Miyata; Hirofumi Miyaji; Hideya Kawasaki; Masaki Yamamoto; Erika Nishida; Hiroko Takita; Tsukasa Akasaka; Natsumi Ushijima; Toshihiko Iwanaga; Tsutomu Sugaya
  International journal of nanomedicine, 12, 2703, 2716, 2017, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal

  
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• 酸化グラフェンの医療応用―再生医療材料の創製と抗菌療法の開発―
  宮治裕史
  月刊Material Stage, 16, 10, 60‐63, 2017, [Invited], [Lead author]
  Japanese
• Effect of protein adsorption on alignment of human gingival fibroblasts on grooved composite resin
  Tsukasa Akasaka; Takuya Imamura; Hirofumi Miyaji; Naoyuki Kaga; Atsuro Yokoyama; Yasuhiro Yoshida
  e-Journal of Surface Science and Nanotechnology, 14, 225, 230, 10 Dec. 2016, [Peer-reviewed]
  English, International conference proceedings
• Periodontal tissue engineering by nano beta-tricalcium phosphate scaffold and fibroblast growth factor-2 in one-wall infrabony defects of dogs
  K. Ogawa; H. Miyaji; A. Kato; Y. Kosen; T. Momose; T. Yoshida; E. Nishida; S. Miyata; S. Murakami; H. Takita; B. Fugetsu; T. Sugaya; M. Kawanami
  JOURNAL OF PERIODONTAL RESEARCH, 51, 6, 758, 767, Dec. 2016, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Adhesion of Osteoblast-like cells (Saos-2) on micro-/submicro-patterned apatite scaffolds fabricated with apatite cement paste by micro-molding
  T Akasaka; H Miyaji; N Kaga; A Yokoyama; S Abe; Y Yoshida
  Nano Biomed, 8, 112, 122, Dec. 2016, [Peer-reviewed]

  
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• Physicochemical fabrication of antibacterial calcium phosphate submicrospheres with dispersed silver nanoparticles via coprecipitation and photoreduction under laser irradiation.
  Maki Nakamura; Ayako Oyane; Yoshiki Shimizu; Saori Miyata; Ayumi Saeki; Hirofumi Miyaji
  Acta biomaterialia, 46, 299, 307, Dec. 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal, We achieved rapid, surfactant-free, and one-pot fabrication of antibacterial calcium phosphate (CaP) submicrospheres containing silver nanoparticles by combining physical laser and chemical coprecipitation processes. In this physicochemical process, weak pulsed laser irradiation (20min) was performed on a labile CaP reaction mixture supplemented with silver ions as a light-absorbing agent. The silver content in the submicrospheres was controlled for a wide range (Ag/P elemental ratio varied from 0.60 to 62.0) by tuning the initial concentration of silver ions (from 5 to 20mM) in the CaP reaction mixture. At the silver concentration of 5mM, we obtained unique nanocomposite particles: CaP submicrospheres (average diameter of approximately 500nm) containing metallic silver nanoparticles dispersed throughout, as a result of CaP and silver coprecipitation with simultaneous photoreduction of silver ions and spheroidization of the coprecipitates. These CaP submicrospheres containing silver nanoparticles (ca. 0.3mg silver per 1mg submicrospheres) exhibited antibacterial activity against major pathogenic oral bacteria, i.e., Streptococcus mutans, Aggregatibacter actinomycetemcomitans, and Porphyromonas gingivalis. Moreover, the CaP submicrospheres dissolved and neutralized the acidic environment generated by Streptococcus mutans, demonstrating their potential as acid-neutralizing and remineralizing agents. The present process and resulting antibacterial CaP-based submicrospheres are expected to be useful in dental healthcare and infection control. STATEMENT OF SIGNIFICANCE: Nano- and microsized spheres of calcium phosphate (CaP) containing silver nanoparticles have great potential in dental applications. Conventional fabrication processes were time-consuming or weak regarding the size/shape control of the spheres. In this study, we achieved a simple (one-pot), rapid (20-min irradiation), and surfactant-free fabrication of CaP submicrospheres containing silver nanoparticles by pulsed laser irradiation to a mixture of calcium, phosphate, and silver ion solutions. The resulting CaP submicrospheres contained metallic silver nanoparticles dispersed throughout in a sequence of reactions: CaP and silver coprecipitation, laser-induced melting and spheroidization of the coprecipitates, and photoreduction of silver ions. These submicrospheres showed antibacterial activity against oral bacteria and acid-neutralizing property in the bacterial suspension, and hence are worth considering for dental applications.
• Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells
  S. Yoshida; N. Wada; D. Hasegawa; H. Miyaji; H. Mitarai; A. Tomokiyo; S. Hamano; H. Maeda
  JOURNAL OF DENTAL RESEARCH, 95, 11, 1282, 1290, Oct. 2016, [Peer-reviewed]
  English, Scientific journal
• Modification of composite resin surface with carbon nanotubes enhances cell proliferation
  S Tanaka; H Miyaji; E Nishida; K Inoue; S Miyata; A Kato; I Kanayama; S Murakami; K Kawamoto; B Fugetsu; T Tanaka; H Takita; T Iizuka; M Kawanami
  Nano Biomed, 8, 27, 34, Oct. 2016, [Peer-reviewed]
  English

  
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• Influence of enamel matrix derivative on healing of root surfaces after bonding treatment and intentional replantation of vertically fractured roots.
  Tsutomu Sugaya; Mahito Tomita; Youji Motoki; Hirofumi Miyaji; Masamitsu Kawamami
  Dental traumatology : official publication of International Association for Dental Traumatology, 32, 5, 397, 401, Wiley-Blackwell, Oct. 2016, [Peer-reviewed], [International Magazine]
  English, Scientific journal, BACKGROUND/AIM: The objectives of this study were to histopathologically evaluate cementum regeneration on root surfaces when an enamel matrix derivative (EMD) was used to bond a vertically fractured root and to evaluate the effectiveness of EMD in inhibiting root resorption. MATERIAL AND METHODS: A total of 40 roots from 24 maxillary premolars in beagles were used. The root was vertically fractured using a chisel and mallet. Super-Bond was then used to bond the fractured root. In the experimental group, the root surface was treated with ethylenediaminetetraacetic acid and an EMD. The control group received no treatment. The root was then replanted in its original location. Histopathological observation and measurement using image-analyzing software were carried out after 8 weeks. RESULTS: In the experimental group, shallow resorption cavities developed on the root planed surfaces with new acellular cementum appearing over them. In the control group, no new cementum was seen on the planed surfaces, and there was connective tissue joining the roots. In some of the samples, resorption and multinucleated giant cells were seen. The experimental group showed a significantly larger volume of cementum formation (P < 0.001), and the volume of root resorption was significantly smaller (P = 0.004). CONCLUSION: When bonding and replanting tooth roots after a vertical fracture, the application of EMD was effective in regenerating cementum on root surfaces from which periodontal ligament had been lost in the area around the fracture line and in reducing the incidence of root resorption.
• β-三リン酸カルシウムナノ粒子配合コラーゲンスキャフォールドのイヌ抜歯窩骨形成促進効果
  Kato A; Miyaji H; Ogawa K; Momose T; Nishida E; Murakami S; Yoshida T; Tanaka S; Sugaya T; Kawanami M
  Jpn J Conserv Dent, 59, 4, 351, 358, (NPO)日本歯科保存学会, Aug. 2016, [Peer-reviewed], [Corresponding author]
  Japanese
• カーボンナノシート新素材『酸化グラフェン』の歯周病治療への展開
  Hirofumi Miyaji; Erika Nishida
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 58, 2, 65, 71, Japanese Society of Periodontology, Jul. 2016, [Invited], [Lead author, Corresponding author]
  Japanese, 歯周病治療において、新しい骨補填材やティッシュエンジニアリング法が開発され、多面的に新技術が応用されつつある。グラファイトから生成されるカーボンナノシート「酸化グラフェン」は、安全性が高く優れた理工学的特性を有するとともに、抗菌性やドラッグデリバリー効果、培養細胞へのポジティブな効果が報告されている。酸化グラフェンについて説明し、酸化グラフェンの細胞活性への効果、酸化グラフェンのティッシュエンジニアリングへの応用、酸化グラフェンの抗菌性評価について述べた。
• Preparation of Chitosan-Gelatin Based Sponge Cross-Linked with GlcNAc for Bone Tissue Engineering
  Thitirat Chaochai; Hirofumi Miyaji; Takashi Yoshida; Erika Nishida; Tetsuya Furuike; Hiroshi Tamura
  Journal of Chitin and Chitosan Science, 4, 1, 1, 8, American Scientific Publishers, 01 Mar. 2016, [Peer-reviewed]
  Scientific journal
• Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket
  Erika Nishida; Hirofumi Miyaji; Akihito Kato; Hiroko Takita; Toshihiko Iwanaga; Takehito Momose; Kosuke Ogawa; Shusuke Murakami; Tsutomu Sugaya; Masamitsu Kawanami
  INTERNATIONAL JOURNAL OF NANOMEDICINE, 11, 2265, 2277, 2016, [Peer-reviewed], [Corresponding author]
  English, Scientific journal

  
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• Sealing the gap of vertical root fracture through the root canal
  T Sugaya; M Nakatsuka; Y Motoki; S Tanaka; H Miyaji; M Kawanami; R Sakagami
  Dentistry, 6, Jan. 2016, [Peer-reviewed]
  English
• Collagen Hydrogel Scaffold and Fibroblast Growth Factor-2 Accelerate Periodontal Healing of Class II Furcation Defects in Dog.
  Takehito Momose; Hirofumi Miyaji; Akihito Kato; Kosuke Ogawa; Takashi Yoshida; Erika Nishida; Syusuke Murakami; Yuta Kosen; Tsutomu Sugaya; Masamitsu Kawanami
  The open dentistry journal, 10, 1, 347, 59, Bentham Science Publishers Ltd., 2016, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, OBJECTIVE: Collagen hydrogel scaffold exhibits bio-safe properties and facilitates periodontal wound healing. However, regenerated tissue volume is insufficient. Fibroblast growth factor-2 (FGF2) up-regulates cell behaviors and subsequent wound healing. We evaluated whether periodontal wound healing is promoted by application of collagen hydrogel scaffold in combination with FGF2 in furcation defects in beagle dogs. METHODS: Collagen hydrogel was fabricated from bovine type I collagen with an ascorbate-copper ion cross-linking system. Collagen hydrogel was mingled with FGF2 and injected into sponge-form collagen. Subsequently, FGF2 (50 µg)/collagen hydrogel scaffold and collagen hydrogel scaffold alone were implanted into class II furcation defects in dogs. In addition, no implantation was performed as a control. Histometric parameters were assessed at 10 days and 4 weeks after surgery. RESULT: FGF2 application to scaffold promoted considerable cell and tissue ingrowth containing numerous cells and blood vessel-like structure at day 10. At 4 weeks, reconstruction of alveolar bone was stimulated by implantation of scaffold loaded with FGF2. Furthermore, periodontal attachment, consisting of cementum-like tissue, periodontal ligament-like tissue and Sharpey's fibers, was also repaired, indicating that FGF2-loaded scaffold guided self-assembly and then re-established the function of periodontal organs. Aberrant healing, such as ankylosis and root resorption, was not observed. CONCLUSION: FGF2-loaded collagen hydrogel scaffold possessed excellent biocompatibility and strongly promoted periodontal tissue engineering, including periodontal attachment re-organization.

  
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• Friction behavior of network-structured CNT coating on pure titanium plate
  Junko Umeda; Bunshi Fugetsu; Erika Nishida; Hirofumi Miyaji; Katsuyoshi Kondoh
  APPLIED SURFACE SCIENCE, 357, 721, 727, Dec. 2015, [Peer-reviewed]
  English, Scientific journal
• Biological response to nanostructure of carbon nanotube/titanium composite surfaces
  E Nishida; H Miyaji; J Umeda; K Kondoh; H Takita; I Kanayama; S Tanaka; A Kato; B Fugetsu; T Akasaka; M Kawanami
  Nano Biomed, 7, 11, 20, Jun. 2015, [Peer-reviewed], [Corresponding author]

  
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• Bone augmentation using a highly porous PLGA/beta-TCP scaffold containing fibroblast growth factor-2
  T. Yoshida; H. Miyaji; K. Otani; K. Inoue; K. Nakane; H. Nishimura; A. Ibara; A. Shimada; K. Ogawa; E. Nishida; T. Sugaya; L. Sun; B. Fugetsu; M. Kawanami
  JOURNAL OF PERIODONTAL RESEARCH, 50, 2, 265, 273, Apr. 2015, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Comparison of fracture sites and post lengths in longitudinal root fractures.
  Tsutomu Sugaya; Megumi Nakatsuka; Kana Inoue; Saori Tanaka; Hirofumi Miyaji; Ryuji Sakagami; Masamitsu Kawamami
  Journal of endodontics, 41, 2, 159, 63, Elsevier {BV}, Feb. 2015, [Peer-reviewed], [International Magazine]
  English, Scientific journal, INTRODUCTION: Comparing the epidemiology of fractures originating in the cervical and apical regions may help to understand the causes and risk of a vertical root fracture. We aimed to determine the frequency of vertical root fractures in different fracture sites and how the fracture site relates to fracture direction and post length. METHODS: Teeth diagnosed with a vertical root fracture were retrospectively surveyed for age and sex of the patient, type of tooth, a fracture region in the longitudinal axial direction, site of the fracture, and presence of a post. The fracture region in the longitudinal axial direction was classified as an incomplete fracture, complete fracture, and uncertain. Incomplete fractures were further classified into a fracture originating in the cervical region, a fracture originating in the midregion, and a fracture originating in the apical region. Posts were evaluated by loss of post and length of post. RESULTS: Fractures originating in the cervical and apical region occurred around the same frequency, whereas fractures originating in the midregion were extremely scarce. Of the fractures originating in the cervical region, 36.2% were in a mesial and/or distal site and 57.4% in a buccal and/or lingual site. Of the fractures originating in the apical region, 90.8% were in the buccal and/or lingual site. The number of cases of fractures originating in the apical region decreased with increased post length. CONCLUSIONS: Sites of fracture and post length differed greatly between fractures originating in the cervical region and the apical region, suggesting that risk factors for fractures originating in the cervical and apical regions are different.
• Combination of Root Surface Modification with BMP-2 and Collagen Hydrogel Scaffold Implantation for Periodontal Healing in Beagle Dogs.
  Akihito Kato; Hirofumi Miyaji; Ryosuke Ishizuka; Keisuke Tokunaga; Kana Inoue; Yuta Kosen; Hiroyuki Yokoyama; Tsutomu Sugaya; Saori Tanaka; Ryuji Sakagami; Masamitsu Kawanami
  The open dentistry journal, 9, 1, 52, 9, Bentham Science Publishers Ltd., 2015, [Peer-reviewed], [Corresponding author], [International Magazine]
  English, Scientific journal, UNLABELLED: Objective : Biomodification of the root surface plays a major role in periodontal wound healing. Root surface modification with bone morphogenetic protein (BMP) stimulates bone and cementum-like tissue formation; however, severe ankylosis is simultaneously observed. Bio-safe collagen hydrogel scaffolds may therefore be useful for supplying periodontal ligament cells and preventing ankylosis. We examined the effects of BMP modification in conjunction with collagen hydrogel scaffold implantation on periodontal wound healing in dogs. MATERIAL AND METHODS: The collagen hydrogel scaffold was composed of type I collagen sponge and collagen hydrogel. One-wall infrabony defects (5 mm in depth, 3 mm in width) were surgically created in six beagle dogs. In the BMP/Col group, BMP-2 was applied to the root surface (loading dose; 1 µg/µl), and the defects were filled with collagen hydrogel scaffold. In the BMP or Col group, BMP-2 coating or scaffold implantation was performed. Histometric parameters were evaluated at 4 weeks after surgery. RESULTS: Single use of BMP stimulated formation of alveolar bone and ankylosis. In contrast, the BMP/Col group frequently enhanced reconstruction of periodontal attachment including cementum-like tissue, periodontal ligament and alveolar bone. The amount of new periodontal ligament in the BMP/Col group was significantly greater when compared to all other groups. In addition, ankylosis was rarely observed in the BMP/Col group. CONCLUSION: The combination method using root surface modification with BMP and collagen hydrogel scaffold implantation facilitated the reestablishment of periodontal attachment. BMP-related ankylosis was suppressed by implantation of collagen hydrogel.

  
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• Graphene oxide coating facilitates the bioactivity of scaffold material for tissue engineering
  Erika Nishida; Hirofumi Miyaji; Hiroko Takita; Izumi Kanayama; Maiko Tsuji; Tsukasa Akasaka; Tsutomu Sugaya; Ryuji Sakagami; Masamitsu Kawanami
  JAPANESE JOURNAL OF APPLIED PHYSICS, 53, 6, 1, 7, Jun. 2014, [Peer-reviewed], [Invited], [Corresponding author]
  English, Scientific journal
• Comparative study of bioactivity of collagen scaffolds coated with graphene oxide and reduced graphene oxide
  Izumi Kanayama; Hirofumi Miyaji; Hiroko Takita; Erika Nishida; Maiko Tsuji; Bunshi Fugetsu; Ling Sun; Kana Inoue; A. Sako Ibara; Tsukasa Akasaka; Tsutomu Sugaya; Masamitsu Kawanami
  INTERNATIONAL JOURNAL OF NANOMEDICINE, 9, 3363, 3373, 2014, [Peer-reviewed], [Corresponding author]
  English, Scientific journal

  
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• Bone augmentation in rat by highly porous β-TCP scaffolds with different open-cell sizes in combination with fibroblast growth factor-2
  H Miyaji; H Yokoyama; Y Kosen; H Nishimura; K Nakane; S Tanaka; K Otani; K Inoue; A Ibara; I Kanayama; T Yoshida; K Ogawa; E Nishida; M Kawanami
  J Oral Tissue Engin, 10, 3, 172, 181, Japanese Association of Regenerative Dentistry, 2013, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal, We prepared highly porous beta-tricalcium phosphate (β-TCP) scaffolds with different open-cell structure sizes. The aim of this study was to examine whether the open-cell size of the scaffold affected osteoinduction in combination with fibroblast growth factor-2 (FGF2) in rats. Polyurethane foam was immersed in β-TCP slurry and sintered in a furnace. Porous β-TCP scaffolds were prepared in three cell sizes (0.6, 0.4 and 0.3 mm) and characterized. Subsequently, each scaffold with FGF2 was implanted to rat cranial bone. Histomorphometric analyses were taken at 35 days post-surgery. The results showed that each β-TCP scaffold exhibited fully interconnected porosity, and frequently allowed bone tissue ingrowth. The 0.4-mm cell sized scaffold significantly promoted bone augmentation compared to the 0.3-mm type. Resorption of the β-TCP scaffold of 0.4-mm cell size was frequently accelerated. In conclusion, FGF2-loaded β-TCP scaffolds with 0.4-mm cell size would be effective for bone tissue engineering.

  
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• Osteoconductivity and Biodegradability of Collagen Scaffold Coated with Nano-beta-TCP and Fibroblast Growth Factor 2
  Asako Ibara; Hirofumi Miyaji; Bunshi Fugetsu; Erika Nishida; Hiroko Takita; Saori Tanaka; Tsutomu Sugaya; Masamitsu Kawanami
  JOURNAL OF NANOMATERIALS, 2013, 1, 11, 2013, [Peer-reviewed], [Corresponding author]
  English, Scientific journal

  
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• Application of collagen hydrogel/sponge scaffold facilitates periodontal wound healing in class II furcation defects in beagle dogs
  Y. Kosen; H. Miyaji; A. Kato; T. Sugaya; M. Kawanami
  JOURNAL OF PERIODONTAL RESEARCH, 47, 5, 626, 634, Oct. 2012, [Peer-reviewed], [Corresponding author]
  English, Scientific journal
• Healing of experimental apical periodontitis after apicoectomy using different sealing materials on the resected root end
  Kaori Otani; Tsutomu Sugaya; Mahito Tomita; Yukiko Hasegawa; Hirofumi Miyaji; Taichi Tenkumo; Saori Tanaka; Youji Motoki; Yasuhiro Takanawa; Masamitsu Kawanami
  DENTAL MATERIALS JOURNAL, 30, 4, 485, 492, Jul. 2011, [Peer-reviewed]
  English, Scientific journal
• Influence of root surface conditioning with bone morphogenetic protein-2 on periodontal wound healing in beagle dogs.
  H Miyaji; T Sugaya; K Ibe; R Ishizuka; K Tokunaga; M Kawanami
  J Oral Tissue Engin, 8, 3, 173, 180, 2011, [Peer-reviewed]
  Bone morphogenetic protein-2 (BMP-2) may play a major role in osteoinduction and osteoclast differentiation. The influence of BMP-2 conditioning in graded doses to root dentin surface on periodontal wound healing was examined.
  
  Twenty-four periodontal defects were surgically created on the buccal roots of four beagle dogs. The denuded root dentin surfaces were demineralized with 24% EDTA, 0.1 and 1.0 µg/µL BMP-2 solution (15 µL) was applied, and the groups were labeled as low-dose BMP (BL) and high-dose BMP (BH), respectively. In the control roots, phosphate-buffered saline was applied to the root surface. Specimens were histologically analyzed 16 weeks after surgery.
  
  Periodontal wound healing was stimulated by conditioning with BMP-2. Formation of alveolar bone and cementum-like tissue was observed; however, in BH group, ankylosis and root resorption were accelerated in the defect area, and there was little evidence of periodontal ligament formation between alveolar bone and root surface.
• Root surface conditioning with bone morphogenetic protein-2 facilitates cementum-like tissue deposition in beagle dogs
  H. Miyaji; T. Sugaya; K. Ibe; R. Ishizuka; K. Tokunaga; M. Kawanami
  JOURNAL OF PERIODONTAL RESEARCH, 45, 5, 658, 663, Oct. 2010, [Peer-reviewed]
  English, Scientific journal
• 歯周組織再生への新しいアプローチ
  宮治裕史; 川浪雅光
  北海道歯学雑誌, 31, 2, 121, 123, 北海道歯学会, 2010, [Invited], [Lead author]
  Japanese

  
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• Ectopic bone induction by BMP-loaded collagen scaffold and bone marrow stromal cell sheet.
  K Inoue; H Miyaji; T Sugaya; M Kawanami
  J Oral Tissue Engin, 8, 1, 19, 29, Japanese Association of Regenerative Dentistry, 2010, [Peer-reviewed]
  English, We combined the use of bone morphogenetic protein-2(BMP-2)-loaded collagen scaffold and cultured bone marrow stromal cell (BMSC) sheet in order for developing new bone tissue engineering.
  Rat BMSC sheets were generated in the presence of dexamethasone, β-glycerophosphate and ascorbic acid. Four types of the grafts were implanted in the intermuscular region of rat femurs: control group, collagen sponge; cell group, BMSC sheet in collagen sponge; BMP group, BMP-2-loaded collagen sponge; and BMP-cell group, BMSC sheet in BMP-2-loaded collagen sponge. Four weeks after surgery, histological and histomorphometric analysis were performed.
  The BMP-cell group demonstrated abundant new bone formation compared to all the other groups (p<0.05) and marked resorption of the implanted collagen when compared to the control and cell groups (p<0.05).
  The combination of BMP-2-loaded collagen sponge and BMSC sheet appeared to successfully promote an osteoinduction.
• Periodontal Healing by Implantation of Collagen Hydrogel-sponge Composite in One-wall Infrabony Defects in Beagle Dogs
  KATO Akihito; MIYAJI Hirofumi; KOSEN Yuta; YOKOYAMA Hiroyuki; ISHIZUKA Ryosuke; TOKUNAGA Keisuke; INOUE Kana; HENMI Masaru; TANAKA Saori; SUGAYA Tsutomu; KAWANAMI Masamitsu
  Journal of Oral Tissue Engineering, 8, 1, 39, 46, Japanese Association of Regenerative Dentistry, 2010, [Peer-reviewed]
  English, The purpose of this study was to evaluate the effect of implanted collagen hydrogel-sponge composite on periodontal wound healing. One-wall infrabony defects (depth: 5 mm; width: 3 mm) were surgically created in three beagle dogs. The exposed root surface was planed and demineralized with EDTA. In the experimental group, the defects were filled with collagen hydrogel-sponge composite. Conversely, no collagen hydrogel-sponge composite was applied to defects in the control group. Histomorphometric parameters were evaluated four weeks after surgery. In the experimental group, regeneration of alveolar bone and cementum was frequently observed. Periodontal ligament tissue was reestablished between the alveolar bone and cementum. New bone height, new bone area, new cementum and new periodontal ligament in the experimental group were significantly greater than those in the control group (p < 0.05). These findings suggest that implanted collagen hydrogel-sponge composite facilitates periodontal wound healing in one-wall infrabony defects in beagle dogs.
• Bone Augmentation by Implantation of an FGF2-loaded Collagen Gel-sponge Composite Scaffold
  KOBAYASHI Naoko; MIYAJI Hirofumi; SUGAYA Tsutomu; KAWANAMI Masamitsu
  Journal of Oral Tissue Engineering, 8, 2, 91, 101, Japanese Association of Regenerative Dentistry, 2010, [Peer-reviewed]
  English, Fibroblast growth factor-2 (FGF2) plays a critical role in osteoblastic cell proliferation. Collagen gel-sponge composite is an effective scaffold for tissue engineering. The purpose of this study was to evaluate whether addition of FGF2 to collagen gel-sponge composite promotes bone augmentation in rats.
  The rats were assigned to groups designated F0, F3 and F15, and received implantation of collagen gel-sponge composite containing 0, 3 and 15 µg FGF2, respectively, into a cranial bone defect. Specimens were prepared 1, 2 and 5 weeks after surgery for histologic and histomorphometric analysis.
  Newly formed bone area in groups F3 and F15 was significantly greater than that in group F0 at all stages. These results suggest that FGF2-loaded collagen gel-sponge composite scaffold stimulates bone augmentation and might provide a more effective bone engineering approach.
• Bone Perforation and Placement of Collagen Sponge Facilitate Bone Augmentation
  Shinji Shimoji; Hirofumi Miyaji; Tsutomu Sugaya; Hifumi Tsuji; Tetsunari Hongo; Megumi Nakatsuka; Khurshid Uz Zaman; Masamitsu Kawanami
  JOURNAL OF PERIODONTOLOGY, 80, 3, 505, 511, Mar. 2009, [Peer-reviewed]
  English, Scientific journal
• Biological Effects of Collagen Hydrogel-sponge Composite Scaffold and Osteoinduction by Combined Application with BMP
  TOKUNAGA Keisuke; SUGAYA Tsutomu; MIYAJI Hirofumi; KAWANAMI Masamitsu
  日本歯科保存学雑誌, 51, 6, 659, 669, 特定非営利法人日本歯科保存学会, Dec. 2008, [Peer-reviewed]
  Japanese, We prepared a collagen hydrogel-sponge composite which would serve as a bioactive scaffold for bone engineering. The present study examined the effects of the collagen composite scaffold combined with/without bone morphogenetic protein (BMP) on tissue reaction and osteoinduction after implantation in rat cranial bone defects. In order to create the collagen hydrogel-sponge composite, 1.5% collagen hydrogel cross-linked by ascorbate and copper ion was injected into 4% FC-HAC sponge (6×6×3mm) which was prepared by mixing fibrillar collagen with heat-denatured collagen. A bone defect (4×4×0.5m...
• 歯周組織再生療法の臨床研究の現況
  川浪雅光; 齋藤彰; 加藤熈; 菅谷勉; 小田島朝臣; 宮治裕史; 齋藤恵美子
  歯科臨床研究, 5, 2, 71, 81, クインテッセンス出版(株), Jul. 2008, [Invited]
  Japanese
• BMPを用いた歯周組織再生療法の開発
  宮治裕史; 菅谷勉; 加藤昭人; 齋藤彰; 小田島朝臣; 齋藤恵美子; 川浪雅光
  日本歯科評論, 68, 4, 39, 40, (株)ヒョーロン・パブリッシャーズ, Apr. 2008, [Invited], [Lead author]
  Japanese
• Effect of BMP-2 application to root dentin surface on periodontal regeneration in 1-wall infrabony defects
  KATO Akihito; MIYAJI Hirofumi; SUGAYA Tsutomu; KAWANAMI Masamitsu
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 49, 4, 296, 304, 特定非営利活動法人 日本歯周病学会, Dec. 2007, [Peer-reviewed]
  Japanese, This study evaluated periodontal regeneration after the application of BMP-2 to root dentin surface in 1-wall infrabony defects.<BR>The mandibular first and third premolars in seven beagle dogs were extracted prior to experimental surgery. Eight weeks after the extraction, the periosteum was removed from the alveolar bone following the retraction of a partial thickness of the muco-gingival flap. Subsequently, 1-wall infrabony defects (5 mm in height, 3 mm in width) were surgically created in the mesial and distal sides of the mandibular second premolars, and in the mesial sides of the mandi...
• Bonding Treatment of Vertical Root Fracture
  SUGAYA Tsutomu; TANAKA Saori; MIYAJI Hirofumi; TOMITA Mahito; HASEGAWA Yukiko; KAWAMURA Naoto; TANAKA Yuko; MOTOKI Youji; NAKATSUKA Megumi; KAWANAMI Masamitsu; KATO Hiroshi; SANO Hidehiko; SIDHU Sharanbir K
  日本歯科医学会誌, 26, 26, 47, 51, 日本歯科医学会, Mar. 2007, [Invited]
  Japanese
• The effects of collagen hydrogel implantation in buccal dehiscence defects in beagles
  H Miyaji; T Sugaya; K Kato; N Kawamura; M Kawanami
  J Oral Tissue Engin, 5, 2, 87, 95, Japanese Association of Regenerative Dentistry, 2007, [Peer-reviewed], [Lead author, Corresponding author]
  English, Type I collagen hydrogel treated by an ascorbate-copper ion crosslinking system is highly biocompatible and highly degradable in the body. The purpose of this study was to examine the effect of collagen hydrogel on periodontal wound healing in beagles. Sixty-four periodontal dehiscence type defects were created on the buccal roots of four beagles. Subsequently, collagen hydrogel was implanted in each defect in the experimental group and sutured, and, in the control group, the defect was sutured without any application of collagen hydrogel. The percentage of the length of new bone and new cementum in the experimental group was significantly greater than that in the control group at weeks 4 (p<0.01) and 8 (p<0.05). Significantly more junctional epithelium was observed in the control group than in the experimental group at weeks 2 (p<0.01) and 4 (p<0.05). These findings thus indicate that periodontal regeneration was stimulated by collagen hydrogel implantation in beagles.

  
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• Suppression of cementum-like tissue formation on BMP-2-applied dentin surface by bisphosphonate
  HONGO Tetsunari; MIYAJI Hirofumi; SUGAYA Tsutomu; KAWANAMI Masamitsu
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 48, 4, 285, 296, 特定非営利活動法人 日本歯周病学会, Dec. 2006, [Peer-reviewed]
  Japanese, We studied the effects of bisphosphonate administration on cementum-like tissue formation and dentin resorption induced by BMP-2 in rats.<BR>Incadronate (1μg/kg/day) was administered intraperitoneally to rats three times per week, from two weeks before implanting dentin blocks to the end of the observation period. Saline was administered under the same protocol without incadronate to the control group. We prepared 180 dentin blocks from rat teeth roots and treated them with 24%EDTA. Each block had 0,100 or 400μg/ml rhBMP-2 applied. Blocks were then implanted into palatal connective tissue o...
• Periodontal regeneration by BMP-2 solution and collagen hydrogel application to root dentin surface
  ISHIZUKA Ryousuke; MIYAJI Hirofumi; SUGAYA Tsutomu; KAWANAMI Masamitsu
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 48, 4, 255, 266, 特定非営利活動法人 日本歯周病学会, Dec. 2006, [Peer-reviewed]
  Japanese, We evaluates periodontal regeneration in a dehiscence model after the application of BMP-2 solution and collagen hydrogel to the root dentin surface.<BR>Materials were 128 premolars of 8 beagles. After a partial thickness muco-gingival flap on the buccal site was elevated, periodontal dehiscence defects with a length of 6 mm were surgically produced. Root surfaces were scaled and planed to remove periodontal ligament and cementum. <BR>Denuded dentin surfaces were subsequently demineralized with 24% EDTA and divided into four groups. In the control group, neither BMP-2 solution nor collagen ...
• Dentin resorption and cementum-like tissue formation by bone morphogenetic protein application
  H Miyaji; T Sugaya; K Kato; N Kawamura; H Tsuji; M Kawanami
  JOURNAL OF PERIODONTAL RESEARCH, 41, 4, 311, 315, Aug. 2006, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Effect of Different Dentin Conditionings on Hard Tissue Formation and Dentin Resorption by rhBMP-2 Application to the Dentin Surface
  Taichi Tenkumo; Hirofumi Miyaji; Tsutomu Sugaya; Masamitsu Kawanami
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 47, 4, 269, 279, (NPO)日本歯周病学会, Dec. 2005, [Peer-reviewed]
  Japanese, Taichi Tenkumo, Hirofumi Miyaji, Tsutomu Sugaya, Masamitsu Kawanami, 2005, 'Effect of Different Dentin Conditionings on Hard Tissue Formation and Dentin Resorption by rhBMP-2 Application to the Dentin Surface', <i>Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)</i>, vol. 47, no. 4, pp. 269-279
• Effect of Transplantation of Bone Marrow Exudate on Bony Augmentation
  TSUJI HIFUMI; SUGAYA TSUTOMU; MIYAJI HIROFUMI; KAWANAMI MASAMITSU
  日本歯科保存学雑誌, 48, 6, 917, 925, (NPO)日本歯科保存学会, Dec. 2005, [Peer-reviewed]
  Japanese, 線維化アテロコラーゲン-熱変性アテロコラーゲン複合体スポンジをラット大腿骨上に移植するさいスポンジに骨髄液を含浸させ,骨増生に及ぼす効果を検討した.移植後,スポンジ内部に多数の紡錘形・楕円形・立法形の細胞および血管の侵入が生じ,骨増生が促進された
• Effect of Decortication and Onlay Graft of Collagen Sponges on Bony Augmentation
  SHIMOJI SHINJI; SUGAYA TSUTOMU; MIYAJI HIROFUMI; KAWANAMI MASAMITSU
  日本歯科保存学雑誌, 48, 6, 926, 938, (NPO)日本歯科保存学会, Dec. 2005, [Peer-reviewed]
  Japanese, ラットの大腿骨に骨髄穿孔を施し穿孔部の上に線維化アテロコラーゲン-熱変性アテロコラーゲン複合体スポンジを埋植した群と,骨髄穿孔はせずにスポンジ埋植のみ行った群,骨髄穿孔のみ行った群,骨膜の剥離のみ行った群を作製し,骨増生の程度を比較した.骨増生の指標として新生骨面積と新生骨の高さを計測した.処置28日後の計測において新生骨面積,新生骨の高さとも骨髄穿孔+スポンジ埋植群が他の3群に比べて有意に高値であった
• Periodontal Regeneration by BMP-2 Application to Root Dentin Surface
  Keisuke Ibe; Hirofumi Miyaji; Tsutomu Sugaya; Masamitsu Kawanami
  Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology), 46, 4, 278, 287, (NPO)日本歯周病学会, Dec. 2004, [Peer-reviewed]
  Japanese, Keisuke Ibe, Hirofumi Miyaji, Tsutomu Sugaya, Masamitsu Kawanami, 2004, 'Periodontal Regeneration by BMP-2 Application to Root Dentin Surface', <i>Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)</i>, vol. 46, no. 4, pp. 278-287
• Influence of Curing Conditions on Tissue Reaction to the 4-META/MMA-TBB Resin
  KAWAMURA NAOTO; SUGAYA TSUTOMU; MIYAJI HIROFUMI; KAWANAMI MASAMITSU
  日本歯科保存学雑誌, 46, 6, 853, 859, (NPO)日本歯科保存学会, Dec. 2003, [Peer-reviewed]
  Japanese, 標題の影響をラットで検討した.空気中でレジンを硬化させると,表面をストリップスで圧接して硬化させた場合や硬化後に表面を研磨した場合に比べて初期の炎症が強かった.骨面を表面処理しレジンを直接塗布して硬化させた場合には炎症性細胞浸潤が殆どみられず,12週後も骨とレジンがハイブリッド様組織を介して接していた.結合組織にレジンを直接塗布して硬化させた場合には結合組織とレジンの界面にハイブリッド様組織がみられ,経時的に炎症性細胞浸潤は消退したものの,多核巨細胞は12週後も残存していた
• Tissue reaction to the surface of 4-META/MMA-TBB resin
  KAWAMURA Naoto; MOTOKI Youji; TSUJI Hifumi; MIYAJI Hirofumi; SUGAYA Tsutomu; KAWANAMI Masamitsu
  日本歯科保存学雑誌, 46, 秋季特別, 46, 46, (NPO)日本歯科保存学会, Oct. 2003, [Peer-reviewed]
  Japanese
• Influence of the Composition and the Concentration of Collagen as a Scaffold for Myeloid Stem Cells on Healing of Bone Defects.
  KATO KATSUYA; SUGAYA TSUTOMU; MIYAJI HIROFUMI; KAWANAMI MASAMITSU
  日本歯科保存学雑誌, 45, 6, 1032, 1043, (NPO)日本歯科保存学会, Dec. 2002, [Peer-reviewed]
  Japanese, 骨髄幹細胞や骨再生に働く細胞のスキャホールドとしての有効性を検討する目的で線維化アテロコラーゲンと熱変性アテロコラーゲンを用いて,組成と濃度の異なるスポンジ状コラーゲンを作成して規格化された人工的骨欠損に移植して骨髄液を浸潤させ,治癒過程に及ぼす影響を検討した.FC-HAC複合体の内部には術後1週では,オステオカルシン陰性の紡錘形の細胞や立方形の細胞が観察され,術後4週では紡錘形の細胞とオステオカルシン陽性の立方形の細胞や血管の増殖が観察され,実験期間を通して炎症性細胞浸潤が少なく,コラーゲン濃度が異なっても治癒状態に差はなかった.FCはコラーゲン濃度を上げるとコラーゲンの吸収が遅延して炎症が強く骨再生を遅延した.2.5〜5%FC-HAC複合体は骨髄幹細胞や骨再生に働く細胞のスキャホールドとして有効であり,歯周組織再生に応用できる可能性が示唆された
• Hard tissue formation on dentin surfaces applied with recombinant human bone morphogenetic protein-2 in the connective tissue of the palate
  H Miyaji; T Sugaya; T Miyamoto; K Kato; H Kato
  JOURNAL OF PERIODONTAL RESEARCH, 37, 3, 204, 209, Jun. 2002, [Peer-reviewed], [Lead author, Corresponding author]
  English, Scientific journal
• Effect of rhBMP-2 Applied Dentin on Alkaline Phosphatase Activity and Mineralized Nodule Formation in Gingival Fibroblasts
  Miyaji Hirofumi; Sugaya Tsutomu; Kato Hiroshi
  Journal of the Japanese Association of Periodontology, 42, 4, 247, 254, 特定非営利活動法人日本歯周病学会, Dec. 2000, [Peer-reviewed], [Lead author]
  Japanese, The purpose of this study was to examine the effects of rhBMP-2 applied dentin on alkaline phosphatase (ALP) activity and mineralized nodule formation in gingival fibroblasts in vitro with a view toward developing periodontal regenerative therapy inducing hard tissue to form on the root surface by gingival connective tissue in vivo. Dentin blocks were demineralized with EDTA, divided into 5 groups, then labeled based on the treatment, i.e., groups O, 1, 5, and 10 with O, 1, 5, and 10 μg/ml rhBMP-2 applied and group PL treated with only D-MEM. Human gingival fibroblasts (HGF) were seeded on ...

• 歯内療法における機能性材料の選択基準と生体適合性
  宮治 裕史, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 163回, 54, 54, Oct. 2025
  (NPO)日本歯科保存学会, Japanese
• アパタイトコーティングおよびアパタイト粒子含浸による2剤担持型歯周組織再生用コラーゲンスキャフォールドの創製
  蔀 佳奈子; Banerjee Kaushita; 大矢根 綾子; 西田 絵利香; 宮治 裕史, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 163回, 181, 181, Oct. 2025
  (NPO)日本歯科保存学会, Japanese
• 歯学研究院における中期目標計画対応の課題と展望
  宮治 裕史; 長谷部 晃, 北海道歯学雑誌, 46, 74, 74, Sep. 2025
  北海道歯学会, Japanese
• Questionnaire survey on clinical training for dental residents at the time of training completion (6th report)
  飯田俊二; 三上紗季; 高師則行; 宮治裕史, 日本歯科医学教育学会総会・学術大会プログラム・抄録集, 44th, 2025
• Fluoride-substituted apatite coating on dental composite resin via light-controlled pseudo-biomineralization
  PONNUSAMY Nandha Kumar; 大矢根綾子; 中村真紀; 猪瀬智也; 小沼一雄; 西田絵利香; 宮治裕史, 日本セラミックス協会年会講演予稿集(Web), 2025, 2025
• 人工手関節全置換術後10年以上の臨床成績
  松井雄一郎; 宮治裕史; 松井雄一郎; 岩崎倫政; 三浪明男, 北海道外科雑誌, 70, 1, 81, 81, 2025
  北海道外科学会, Japanese
• S-PRGナノフィラーバイオリキッドによる抗バイオフィルム性能の評価
  西山和那; 西田絵利香; 宮治裕史; 西田絵利香; 宮治裕史, 北海道外科雑誌, 70, 1, 77, 78, 2025
  北海道外科学会, Japanese
• 酸化グラフェン超薄膜コーティングへの抗菌薬の保持と口腔細菌に対する抗菌効果
  石井花英; 西田絵利香; 宮治裕史; 郷田隼, 北海道外科雑誌, 70, 1, 77, 77, 2025
  北海道外科学会, Japanese
• 人工手関節の開発から臨床応用へ-術後10年の臨床成績に基づく知見-
  松井雄一郎; 松井雄一郎; 三浪明男; 近藤真; 石川淳一; 本宮真; 遠藤健; 宮治裕史; 岩崎倫政, 日本骨形態計測学会雑誌, 35, 1, 2025
• Rapid generation of fluoridated apatite coating on human dentin by laser-assisted biomimetic process with indocyanine green
  蔀佳奈子; 田中佐織; 大矢根綾子; 宮治裕史, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 161回, 90, 90, Nov. 2024
  (NPO)日本歯科保存学会, Japanese
• The effect of SWEEPS laser-activated irrigation on removing of intracanal medicaments
  月田佳李; 西田絵利香; 友清淳; 宮治裕史, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 161回, 99, 99, Nov. 2024
  (NPO)日本歯科保存学会, Japanese
• 酸化グラフェンを用いた細胞成長因子デリバリースキャフォールドの評価(Characterization of drag delivery using graphene oxide scaffold)
  孫 偉浩; 祐田 明香; 宮治 裕史; 林 佳文; 御手洗 裕美; 王 恕心; 冉 子晴; 前田 英史; 和田 尚久, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 161回, 151, 151, Nov. 2024
  (NPO)日本歯科保存学会, English
• シプロフロキサシンを担持したリン酸カルシウム粒子の創製と抗菌評価
  西田 絵利香; Pal Aniruddha; 猪瀬 智也; 中村 真紀; 大矢根 綾子; 宮治 裕史, 日本歯周病学会会誌, 66, 秋季特別, 152, 152, Oct. 2024
  (NPO)日本歯周病学会, Japanese
• 酸化グラフェン超薄膜コーティングへの含嗽剤複合化による抗菌効果の評価
  石井 花英; 西田 絵利香; 田中 佐織; 宮治 裕史; 金本 佑生実; 郷田 隼, 北海道外科雑誌, 69, 1, 72, 72, Jun. 2024
  北海道外科学会, Japanese
• シプロフロキサシンを担持したリン酸カルシウム粒子の創製と抗菌評価
  西田絵利香; PAL Aniruddha; 猪瀬智也; 中村真紀; 大矢根綾子; 宮治裕史, 日本歯周病学会会誌(Web), 66, 2024
• Rapid in situ production of artificial enamel via laser-assisted pseudo-biomineralization
  大矢根綾子; 小沼一雄; 中村真紀; 田中佐織; 西田絵利香; 宮治裕史, 日本セラミックス協会秋季シンポジウム講演予稿集(Web), 37th, 2024
• Calcium phosphate particles immobilizing ciprofloxacin as an antibacterial agent
  中村真紀; PAL Aniruddha; 猪瀬智也; 大矢根綾子; 西田絵利香; 宮治裕史, 日本セラミックス協会秋季シンポジウム講演予稿集(Web), 37th, 2024
• Calcium phosphate nanoparticles immobilizing antibacterial protein
  中村真紀; 分領和歌子; 大矢根綾子; 金本佑生実; 宮治裕史, 日本セラミックス協会年会講演予稿集(Web), 2024, 2024
• 過飽和液中レーザー照射法によるヒト歯根表面の機能化
  田中 佐織; 宮治 裕史; 大矢根 綾子, 日本レーザー歯学会誌, 33, 3, 45, 45, Jan. 2024
  (一社)日本レーザー歯学会, Japanese
• Formation of Various Actin Structures by Osteoclast-like Cells in Response to Micro- and Nano-Patterns
  赤坂司; 宮治裕史; 横山敦郎; 吉田靖弘, 日本バイオマテリアル学会大会予稿集(Web), 45回, 422, 422, Oct. 2023
  日本バイオマテリアル学会, Japanese
• ラット骨窩洞内に埋植した水酸化カルシウム系根管充填剤に対する組織反応
  田中 佐織; 宮治 裕史; 加藤 昭人; 金本 佑生実; 菅谷 勉, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 158回, 84, 84, May 2023
  (NPO)日本歯科保存学会, Japanese
• ヒトI型コラーゲン様リコンビナントペプチド/β-三リン酸カルシウム骨補填材による歯周組織再生効果
  吉野 友都; 宮治 裕史; 西田 絵利香; 金本 佑生実; 浜本 朝子; 加藤 昭人; 菅谷 勉; 赤坂 司, 日本歯周病学会会誌, 65, 春季特別, 133, 133, Apr. 2023
  (NPO)日本歯周病学会, Japanese
• 過飽和液中レーザー照射法によるヒト歯根表面の機能化
  田中佐織; 宮治裕史; 大矢根綾子, 日本レーザー医学会誌(Web), 44, 3, 2023
• 歯科臨床研究と教育の未来を切り拓く
  宮治裕史, 北海道歯学会学術大会プログラム講演内容抄録, 2023, 2023
• ヒトI型コラーゲン様リコンビナントペプチド/β-三リン酸カルシウム骨補填材による歯周組織再生効果
  吉野友都; 宮治裕史; 西田絵利香; 金本佑生実; 浜本朝子; 加藤昭人; 菅谷勉; 赤坂司, 日本歯周病学会会誌(Web), 65, 2023
• 酸化グラフェンとカオチン界面活性剤による透明超薄膜コーティングの持続的抗菌効果
  宮治 裕史; 金本 佑生実; 浜本 朝子; 蔀 佳奈子; 西田 絵利香; 加藤 昭人; 菅谷 勉; 田中 佐織; 相川 夏葉; 川崎 英也; 郷田 隼; 小野 博信, 日本バイオマテリアル学会大会予稿集, 44回, 394, 394, Nov. 2022
  日本バイオマテリアル学会, Japanese
• 光スタンプレーザー転写(LIFTOP)プロセスによる歯表面の改質
  宮治 裕史; 奈良崎 愛子; 大矢根 綾子; 菅谷 勉; 田中 佐織, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 157回, 182, 182, Oct. 2022
  (NPO)日本歯科保存学会, Japanese
• 光スタンプレーザー転写(LIFTOP)プロセスによる歯表面の改質
  宮治 裕史; 奈良崎 愛子; 大矢根 綾子; 菅谷 勉; 田中 佐織, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 157回, 182, 182, Oct. 2022
  (NPO)日本歯科保存学会, Japanese
• 前駆体利用バイオミメティックプロセスによる低結晶性アパタイト成膜足場材 骨・歯周組織形成能の評価
  金本 佑生実; 宮治 裕史; 西田 絵利香; 宮田 さほり; 眞弓 佳代子; 吉野 友都; 加藤 昭人; 菅谷 勉; 赤坂 司; 大矢根 綾子, 日本歯周病学会会誌, 64, 秋季特別, 122, 122, Aug. 2022
  (NPO)日本歯周病学会, Japanese
• Surface pre-reacted glass-ionomer(S-PRG)ナノフィラー分散液を注水に用いた超音波スケーラーによる歯周炎改善効果
  宮治 裕史; 眞弓 佳代子; 金本 佑生実; 岡本 一絵; 浜本 朝子; 加藤 昭人; 菅谷 勉; 赤坂 司; 田中 佐織, 日本歯周病学会会誌, 64, 秋季特別, 126, 126, Aug. 2022
  (NPO)日本歯周病学会, Japanese
• 前駆体利用バイオミメティックプロセスによる低結晶性アパタイト成膜足場材 骨・歯周組織形成能の評価
  金本 佑生実; 宮治 裕史; 西田 絵利香; 宮田 さほり; 眞弓 佳代子; 吉野 友都; 加藤 昭人; 菅谷 勉; 赤坂 司; 大矢根 綾子, 日本歯周病学会会誌, 64, 秋季特別, 122, 122, Aug. 2022
  (NPO)日本歯周病学会, Japanese
• Surface pre-reacted glass-ionomer(S-PRG)ナノフィラー分散液を注水に用いた超音波スケーラーによる歯周炎改善効果
  宮治 裕史; 眞弓 佳代子; 金本 佑生実; 岡本 一絵; 浜本 朝子; 加藤 昭人; 菅谷 勉; 赤坂 司; 田中 佐織, 日本歯周病学会会誌, 64, 秋季特別, 126, 126, Aug. 2022
  (NPO)日本歯周病学会, Japanese
• Surface pre-reacted glass-ionomer(S-PRG)フィラー配合水硬性仮封材の抗菌特性
  宮治 裕史; 金本 佑生実; 菅谷 勉; 田中 佐織, 日本歯内療法学会学術大会プログラム・抄録集, 43回, 61, 61, Jul. 2022
  (一社)日本歯内療法学会, Japanese
• Surface pre-reacted glass-ionomer(S-PRG)フィラー配合水硬性仮封材の抗菌特性
  宮治 裕史; 金本 佑生実; 菅谷 勉; 田中 佐織, 日本歯内療法学会学術大会プログラム・抄録集, 43回, 61, 61, Jul. 2022
  (一社)日本歯内療法学会, Japanese
• 酸化グラフェン超薄膜コーティングへの口腔洗浄含嗽剤の複合化による持続性抗菌効果
  宮治 裕史; 金本 佑生実; 浜本 朝子; 西田 絵利香; 加藤 昭人; 菅谷 勉; 田中 佐織; 郷田 隼; 小野 博信, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 156回, 89, 89, May 2022
  (NPO)日本歯科保存学会, Japanese
• 臼歯部咬頭干渉による咬合性外傷に咬合再構成で対応した慢性歯周炎症例
  宮治 裕史; 菅谷 勉, 日本歯周病学会会誌, 64, 春季特別, 137, 137, May 2022
  (NPO)日本歯周病学会, Japanese
• 酸化グラフェン超薄膜コーティングへの口腔洗浄含嗽剤の複合化による持続性抗菌効果
  宮治 裕史; 金本 佑生実; 浜本 朝子; 西田 絵利香; 加藤 昭人; 菅谷 勉; 田中 佐織; 郷田 隼; 小野 博信, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 156回, 89, 89, May 2022
  (NPO)日本歯科保存学会, Japanese
• 歯周ポケット内の持続的な除菌治療のための多機能性ナノ粒子の創製
  中村 真紀; 大矢根 綾子; 宮治 裕史, 医科学応用研究財団研究報告, 39, 114, 117, Feb. 2022
  (公財)鈴木謙三記念医科学応用研究財団, Japanese
• In Vivo Inflammatory Effects and Surface Composition Changes in Implanted Root Canal Sealer Containing Bioactive Glass
  加藤昭人; 宮治裕史; 吉野友都; 金本佑生実; 浜本朝子; 西田絵利香; 菅谷勉; 田中佐織; 田中佐織, 日本歯科保存学雑誌(Web), 65, 2, 2022
• Fluoride-incorporated Apatite Coating on Tooth Surface via Dental Diode Laser Irradiation in a Supersaturated Solution
  大矢根綾子; 牧野雅; 坂巻育子; 中村真紀; 田中佐織; 宮治裕史, 日本バイオマテリアル学会大会予稿集(Web), 44th, 136, 136, 2022
  日本バイオマテリアル学会, Japanese
• Laser-induced forward transfer of a protein-immobilized apatite film for tooth surface modification
  大矢根綾子; 奈良崎愛子; 宮治裕史, 日本セラミックス協会秋季シンポジウム講演予稿集(Web), 35th, 2022
• Surface functionalization of human cementum by laser irradiation in supersaturated solutions
  田中佐織; 宮治裕史; 大矢根綾子, 日本レーザー歯学会総会・学術大会プログラム・講演抄録集, 34th, 3, 53, 53, 2022
  (一社)日本レーザー歯学会, Japanese
• バイオミネラリゼーションの光制御による高機能化歯面の構築
  大矢根綾子; 宮治裕史, 東北大学金属材料研究所共同研究ワークショップ/日本バイオマテリアル学会東北ブロック講演会, 2022, 2022
• Antibacterial ability of hydraulic temporary sealing material containing surface pre-reacted glass-ionomer (S-PRG) fillers
  宮治裕史; 金本佑生実; 菅谷勉; 田中佐織; 田中佐織, 日本歯内療法学会学術大会プログラム・抄録集, 43rd, 2022
• 前駆体利用バイオミメティックプロセスによる低結晶性アパタイト成膜足場材-骨・歯周組織形成能の評価-
  金本佑生実; 宮治裕史; 西田絵利香; 宮田さほり; 眞弓佳代子; 吉野友都; 加藤昭人; 菅谷勉; 赤坂司; 大矢根綾子, 日本歯周病学会会誌(Web), 64, 2022
• Bone tissue regeneration by collagen scaffolds coated with calcium phosphates with different crystalline structures
  大矢根綾子; SANTHAKUMAR S.; 中村真紀; 金本佑生実; 吉野友都; ALRUWAILI M.K.; 宮治裕史, 日本セラミックス協会年会講演予稿集(Web), 2022, 2022
• 光+超音波ダイナミック療法による歯周病治療に向けた量子ドット光/音増感剤の開発
  川崎英也; 山本健; 宮治裕史, 技苑, 154, 2022
• Toward the Application of ’Light’ Bactericidal Treatment of Periodontal Diseases Highly efficient singlet oxygen generation by rose bengal/biosilica composite
  森七星; 川崎英也; 西田絵利香; 宮治裕史; 梅田純子; 近藤勝義, ナノ学会大会講演予稿集, 20th, 2022
• 臼歯部咬頭干渉による咬合性外傷に咬合再構成で対応した慢性歯周炎症例
  宮治裕史; 菅谷勉, 日本歯周病学会会誌(Web), 64, 2022
• 歯周病治療に向けた量子ドット光・音増感剤の開発
  川崎英也; 山本健; 宮治裕史, 関西大学先端科学技術シンポジウム講演集, 26th, 2022
• 歯周ポケット内の持続的な除菌治療のための多機能性ナノ粒子の創製
  中村真紀; 大矢根綾子; 宮治裕史, 医科学応用研究財団研究報告(CD-ROM), 39, 2022
• 酸化グラフェン/カチオン界面活性剤複合超薄膜の創製と抗菌評価
  宮治 裕史; 浜本 朝子; 西田 絵利香; 金本 佑生実; 菅谷 勉; 蔀 佳奈子; 郷田 隼; 小野 博信, 日本バイオマテリアル学会大会予稿集, 43回, JP2, 34, Nov. 2021
  日本バイオマテリアル学会, Japanese
• 骨再生におけるバイオマテリアル開発と骨質評価アップデート ヒトI型コラーゲン様リコビナントペプチドを用いた骨補填材の開発(Development of bone graft material using recombinant peptide based on human collagen type I)
  宮治 裕史, Journal of Oral Biosciences Supplement, 2021, 89, 89, Oct. 2021
  (一社)歯科基礎医学会, Japanese
• リゾチーム-金ナノクラスター/ローズベンガル複合体の抗菌・抗バイオフィルム光線力学的活性
  岡本 一絵; 宮治 裕史; 宮田 さほり; 菅谷 勉; 蔀 佳奈子; 赤坂 司; 齋田 慧; 川崎 英也, 日本歯周病学会会誌, 63, 秋季特別, 133, 133, Oct. 2021
  (NPO)日本歯周病学会, Japanese
• S-PRGナノフィラーを用いた抗菌性歯面コーティングによる臨床パラメーターの改善 イヌ実験的歯周炎における検討
  眞弓 佳代子; 宮治 裕史; 宮田 さほり; 西田 絵利香; 降旗 友和; 金本 佑生実; 菅谷 勉; 蔀 佳奈子; 赤坂 司, 日本歯周病学会会誌, 63, 秋季特別, 133, 133, Oct. 2021
  (NPO)日本歯周病学会, Japanese
• 酸化グラフェン/塩化セチルピリジニウム複合超薄膜による歯面コーティングと抗菌評価
  宮治 裕史; 浜本 朝子; 西田 絵利香; 菅谷 勉; 蔀 佳奈子; 郷田 隼; 小野 博信, 日本歯周病学会会誌, 63, 秋季特別, 136, 136, Oct. 2021
  (NPO)日本歯周病学会, Japanese
• 酸化グラフェン/塩化セチルピリジニウム複合超薄膜による歯面コーティングと抗菌評価
  宮治裕史; 浜本朝子; 西田絵利香; 菅谷勉; 蔀佳奈子; 郷田隼; 小野博信, 日本歯周病学会会誌(Web), 63, 2021
• S-PRGナノフィラーを用いた抗菌性歯面コーティングによる臨床パラメーターの改善-イヌ実験的歯周炎における検討-
  眞弓佳代子; 宮治裕史; 宮田さほり; 西田絵利香; 降旗友和; 金本佑生実; 菅谷勉; 蔀佳奈子; 赤坂司, 日本歯周病学会会誌(Web), 63, 2021
• リゾチーム-金ナノクラスター/ローズベンガル複合体の抗菌・抗バイオフィルム光線力学的活性
  岡本一絵; 宮治裕史; 宮田さほり; 菅谷勉; 蔀佳奈子; 赤坂司; 齋田慧; 川崎英也, 日本歯周病学会会誌(Web), 63, 2021
• Development of new photoantibacterial agent using rose bengal/biosilica complex
  森七星; 川崎英也; 宮治裕史; 梅田純子; 近藤勝義, ナノ学会大会講演予稿集, 19th, 2021
• Facile apatite coating on a human dentin substrate utilizing dental diode laser
  大矢根綾子; 坂巻育子; 古賀健司; 中村真紀; 蔀佳奈子; 田中佐織; 宮治裕史, 日本セラミックス協会秋季シンポジウム講演予稿集(Web), 34th, 2021
• Simple apatite coating on dental resin via light-curing for improved cell compatibility
  大矢根綾子; 日比野靖; 日比野靖; 蔀佳奈子; 宮治裕史, 日本セラミックス協会年会講演予稿集(Web), 2021, 2021
• Preparation and antibacterial evaluation of graphene oxide/cationic surfactant ultrathin films.
  宮治裕史; 浜本朝子; 西田絵利香; 金本佑生実; 菅谷勉; 蔀佳奈子; 郷田隼; 小野博信, 日本バイオマテリアル学会大会予稿集(Web), 43rd, JP2, 34, 2021
  日本バイオマテリアル学会, Japanese
• Surface functionalization of human tooth cementum and enamel by laser irradiation in a supersaturated calcium phosphate solution
  大矢根綾子; 坂巻育子; 中村真紀; 田中佐織; 田中享; 宮治裕史, 日本バイオマテリアル学会大会予稿集(Web), 43rd, JP2, 05, 2021
  日本バイオマテリアル学会, Japanese
• Elemental composition analysis of root canal sealer containing bioactive glass implanted into rat subcutaneous tissue
  宮治裕史; 吉野友都; 加藤昭人; 菅谷勉; 田中佐織; 田中佐織, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 154th, 117, 117, 2021
  (NPO)日本歯科保存学会, Japanese
• The hardness of human tooth cementum coated with fluoride-incorporated calcium phosphate by laser irradiation in supersaturated solution
  田中佐織; 田中佐織; 宮治裕史; 田中享; 井上哲; 大矢根綾子, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 154th, 94, 94, 2021
  (NPO)日本歯科保存学会, Japanese
• Creation of antibacterial bonding agents containing Zinc-F nanofiller and Ag complex
  蔀佳奈子; 宮治裕史; 堀池康太; 川崎英也; 古市保志, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 154th, 92, 92, 2021
  (NPO)日本歯科保存学会, Japanese
• 過飽和液中レーザー照射法によるセメント質へのフッ素担持リン酸カルシウム成膜
  田中 佐織; 宮治 裕史; 田中 享; 井上 哲; Santhakumar Syama; 大矢根 綾子, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 153回, 56, 56, Nov. 2020
  (NPO)日本歯科保存学会, Japanese
• キャナルスペーストのユージノール放出量の検討
  宮治 裕史; 金本 佑生実; 菅谷 勉; 蔀 佳奈子; 田中 佐織, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 153回, 72, 72, Nov. 2020
  (NPO)日本歯科保存学会, Japanese
• クリニカル・アドバンス 2ペーストタイプ根管充填シーラー「キャナルスペースト」の抗菌性、細胞親和性評価
  宮治 裕史, DENTAL DIAMOND, 45, 11, 152, 154, Aug. 2020
  (株)デンタルダイヤモンド社, Japanese
• 過飽和液中レーザー照射法による象牙質表面へのフッ素担持アパタイト成膜と抗菌特性
  蔀 佳奈子; 宮治 裕史; 菅谷 勉; 古賀 健司; 中村 真紀; 大矢根 綾子, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 152回, 80, 80, Jun. 2020
  (NPO)日本歯科保存学会, Japanese
• Bioactive Glass配合根管充填シーラーの生体親和性評価
  吉野 友都; 宮治 裕史; 金本 佑生実; 浜本 朝子; 西田 絵利香; 加藤 昭人; 菅谷 勉; 田中 佐織, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 152回, 103, 103, Jun. 2020
  (NPO)日本歯科保存学会, Japanese
• 金銀クラスター/ローズベンガル含有キトサンナノゲル複合体の光線力学的特性
  浜本 朝子; 加藤 昭人; 宮田 さほり; 蔀 佳奈子; 宮治 裕史; 菅谷 勉; 齋田 慧; 川崎 英也, 北海道外科雑誌, 65, 1, 91, 91, Jun. 2020
  北海道外科学会, Japanese
• 過飽和液中レーザー照射法による象牙質表面へのフッ素担持アパタイト成膜と抗菌特性
  蔀 佳奈子; 宮治 裕史; 菅谷 勉; 古賀 健司; 中村 真紀; 大矢根 綾子, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 152回, 80, 80, Jun. 2020
  (NPO)日本歯科保存学会, Japanese
• Simple surface functionalization of light-cured dental resin using an apatite-coated film
  日比野靖; 日比野靖; 大矢根綾子; 荒木裕子; 蔀佳奈子; 宮治裕史, 日本セラミックス協会年会講演予稿集(CD-ROM), 2020, 2020
• Material Delivery and Interface Formation by Laser-Induced Forward Transfer
  奈良崎愛子; 中田芳樹; 東海林竜也; 坪井泰之; 佐藤正健; 新納弘之; 大矢根綾子; 宮治裕史, 応用物理学会春季学術講演会講演予稿集(CD-ROM), 67th, 2020
• 難治性歯周病治療への応用に向けた光/音増感剤の開発
  川崎英也; 宮治裕史, 関西大学先端科学技術シンポジウム講演集, 24th, 2020
• Prospects for Material Delivery and Interface Formation by LIFT
  奈良崎愛子; 中田芳樹; 東海林竜也; 坪井泰之; 佐藤正健; 新納弘之; 大矢根綾子; 宮治裕史, 応用物理学会秋季学術講演会講演予稿集(CD-ROM), 81st, 2020
• 金銀クラスター/ローズベンガル含有キトサンナノゲル複合体の光線力学的特性
  浜本朝子; 加藤昭人; 宮田さほり; 蔀佳奈子; 宮治裕史; 菅谷勉; 齋田慧; 川崎英也, 北海道外科雑誌, 65, 1, 91, 91, 2020
  北海道外科学会, Japanese
• Liquid-phase bonding of a microporous apatite film onto a light-cured composite resin
  日比野靖; 日比野靖; 大矢根綾子; 荒木裕子; 宮治裕史, 日本バイオマテリアル学会大会予稿集(Web), 41回, 363, 363, Nov. 2019
  日本バイオマテリアル学会, Japanese
• β-TCPナノ粒子配合ヒトI型コラーゲン様リコンビナントペプチド骨補填材による骨形成効果
  降籏 友和; 宮治 裕史; 西田 絵利香; 加藤 昭人; 宮田 さほり; 蔀 佳奈子; 眞弓 佳代子; 金本 佑生実; 菅谷 勉; 赤坂 司, 日本歯周病学会会誌, 61, 秋季特別, 126, 126, Oct. 2019
  (NPO)日本歯周病学会, Japanese
• ナノS-PRGフィラー分散液と超音波スケーラー注水システムによる抗菌性歯面の構築 フィラーの歯面付着性の検討
  眞弓 佳代子; 宮治 裕史; 岡本 一絵; 加藤 昭人; 菅谷 勉, 日本歯周病学会会誌, 61, 秋季特別, 143, 143, Oct. 2019
  (NPO)日本歯周病学会, Japanese
• キャナルスペーストチューブおよびキャナルスペーストダブルシリンジの抗菌性ならびに生体親和性評価
  金本 佑生実; 宮治 裕史; 蔀 佳奈子; 岡本 一絵; 吉野 友都; 浜本 朝子; 菅谷 勉; 田中 佐織, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 151回, 119, 119, Oct. 2019
  (NPO)日本歯科保存学会, Japanese
• β-TCPナノ粒子配合ヒトI型コラーゲン様リコンビナントペプチド骨補填材による骨形成効果
  降籏 友和; 宮治 裕史; 西田 絵利香; 加藤 昭人; 宮田 さほり; 蔀 佳奈子; 眞弓 佳代子; 金本 佑生実; 菅谷 勉; 赤坂 司, 日本歯周病学会会誌, 61, 秋季特別, 126, 126, Oct. 2019
  (NPO)日本歯周病学会, Japanese
• ナノS-PRGフィラー分散液と超音波スケーラー注水システムによる抗菌性歯面の構築 フィラーの歯面付着性の検討
  眞弓 佳代子; 宮治 裕史; 岡本 一絵; 加藤 昭人; 菅谷 勉, 日本歯周病学会会誌, 61, 秋季特別, 143, 143, Oct. 2019
  (NPO)日本歯周病学会, Japanese
• キャナルスペーストチューブおよびキャナルスペーストダブルシリンジの抗菌性ならびに生体親和性評価
  金本 佑生実; 宮治 裕史; 蔀 佳奈子; 岡本 一絵; 吉野 友都; 浜本 朝子; 菅谷 勉; 田中 佐織, 特定非営利活動法人日本歯科保存学会学術大会プログラムおよび講演抄録集, 151回, 119, 119, Oct. 2019
  (NPO)日本歯科保存学会, Japanese
• β‐TCPナノ粒子配合ヒトI型コラーゲン様リコンビナントペブチド骨補填材による骨形成効果
  降籏友和; 宮治裕史; 西田絵利香; 加藤昭人; 宮田さほり; 蔀佳奈子; 眞弓佳代子; 金本佑生実; 菅谷勉; 赤坂司, 日本歯周病学会会誌(Web), 61, 126, 01 Oct. 2019
  Japanese
• ナノS‐PRGフィラー分散液と超音波スケーラー注水システムによる抗菌性歯面の構築―フィラーの歯面付着性の検討―
  眞弓佳代子; 宮治裕史; 岡本一絵; 加藤昭人; 菅谷勉, 日本歯周病学会会誌(Web), 61, 143, 01 Oct. 2019
  Japanese
• 重度歯周炎と精神遅滞を伴う成人上顎前突症例
  菅原 由紀; 宮治 裕史; 佐藤 嘉晃, 北海道歯学雑誌, 39, 2, 137, 145, Mar. 2019
  北海道歯学会, Japanese
• 抗菌性樹脂用途向けの銀ナノクラスター/第四級アンモニウムカチオン複合体の合成
  川崎英也; 富永千明; 蔀佳奈子; 宮治裕史, 日本化学会春季年会講演予稿集(CD-ROM), 99th, ROMBUNNO.1I2‐40, 01 Mar. 2019
  Japanese
• 過飽和液中レーザー照射法を利用したヒト象牙質基材へのフッ素担持アパタイト成膜
  大矢根綾子; 坂巻育子; 中村真紀; 古賀健司; 蔀佳奈子; 眞弓佳代子; 宮治裕史, 日本セラミックス協会年会講演予稿集(CD-ROM), 2019, 2019
• Antibacterial property of (AuAg NCs, Rose Bengal)@Chitosan nanogel
  加藤昭人; 宮治裕史; 齋田慧; 川崎英也, 日本バイオマテリアル学会大会予稿集(Web), 41st, 367, 367, 2019
  日本バイオマテリアル学会, Japanese
• Bone forming ability of human type-I collagen-like recombinant peptide granules and submicron β-TCP
  西田絵利香; 宮治裕史; 降籏友和; 加藤昭人; 宮田さほり; 蔀佳奈子; 眞弓佳代子; 金本佑生実; 菅谷勉; 赤坂司, 日本バイオマテリアル学会大会予稿集(Web), 41st, 369, 369, 2019
  日本バイオマテリアル学会, Japanese
• Collagen sponge loaded with graphene oxide promotes wound healing in rat skin defect
  宮治裕史; 西田絵利香, 日本バイオマテリアル学会大会予稿集(Web), 41st, 377, 377, 2019
  日本バイオマテリアル学会, Japanese
• ナノβ‐TCP/ヒトI型コラーゲン様リコンビナントペプチド顆粒の骨形成特性評価
  西田絵利香; 宮治裕史; 降籏友和; 宮田さほり; 加藤昭人; 金本佑生実; 菅谷勉; 赤坂司, 日本再生医療学会総会(Web), 18th, ROMBUNNO.P‐02‐099 (WEB ONLY), 2019
  Japanese
• Laser-Induced Forward Transfer of Cell Adhesion Factor Fibronectin-Immobilizing Films Fabricated by Biomimetic Method
  奈良崎愛子; 大矢根綾子; 小室智稀; 小室智稀; 黒崎諒三; 亀山智子; 坂巻育子; 荒木裕子; 海野昌喜; 宮治裕史, 電気学会研究会資料, 2019, OQD-19-007-015, 25, 29, 2019
  電気学会, Japanese
• カーボンナノチューブによるチタン一生体界面のナノスケール改質と骨形成接合効果
  西田 絵利香; 宮治 裕史; 蔀 佳奈子; 眞弓 佳代子; 菅谷 勉; 梅田 純子; 近藤 勝義; 古月 文志, 日本再生歯科医学会誌, 16, 1, 49, 49, Dec. 2018
  日本再生歯科医学会, Japanese
• 過飽和液中レーザー照射法による象牙質表面へのフッ素担持アパタイトの迅速成膜
  大矢根 綾子; 坂巻 育子; 中村 真紀; 古賀 健司; 蔀 佳奈子; 眞弓 佳代子; 宮治 裕史, 日本バイオマテリアル学会大会予稿集, 40回, 129, 129, Nov. 2018
  日本バイオマテリアル学会, Japanese
• 酸化グラフェンスキャフォールドへの亜鉛担持による抗菌性の向上
  西田 絵利香; 宮治 裕史; 蔀 佳奈子; 菅谷 勉; 小野 博信, 日本バイオマテリアル学会大会予稿集, 40回, 274, 274, Nov. 2018
  日本バイオマテリアル学会, Japanese
• 過飽和液中レーザー照射法による象牙質表面へのフッ素担持アパタイトの迅速成膜
  大矢根綾子; 坂巻育子; 中村真紀; 古賀健司; 蔀佳奈子; 眞弓佳代子; 宮治裕史, 日本バイオマテリアル学会大会予稿集(Web), 40th, 129 (WEB ONLY), 129, Nov. 2018
  日本バイオマテリアル学会, Japanese
• シンバスタチン添加α-TCP微粒子エマルション骨ペーストの特性評価および骨形成効果
  舘山 彰人; 加藤 昭人; 宮治 裕史; 西田 絵利香; 岩崎 泰彦; 藤井 秀司; 川本 康平; 蔀 佳奈子; 降籏 友和; 眞弓 佳代子; 菅谷 勉, 日本歯周病学会会誌, 60, 秋季特別, 119, 119, Oct. 2018
  (NPO)日本歯周病学会, Japanese
• シンバスタチン添加α‐TCP微粒子エマルション骨ペーストの特性評価および骨形成効果
  舘山彰人; 加藤昭人; 宮治裕史; 西田絵利香; 岩崎泰彦; 藤井秀司; 藤井秀司; 川本康平; 蔀佳奈子; 降籏友和; 眞弓佳代子; 菅谷勉, 日本歯周病学会会誌(Web), 60, 119, 01 Oct. 2018
  Japanese
• 過飽和液中レーザー照射による象牙質表面への迅速リン酸カルシウム成膜
  大矢根綾子; NATHANAEL A. Joseph; 中村真紀; 眞弓佳代子; 蔀佳奈子; 宮治裕史, 日本セラミックス協会秋季シンポジウム講演予稿集(CD-ROM), 31st, ROMBUNNO.3U07, 27 Aug. 2018
  Japanese
• S‐PRGフィラー含有根管充填シーラーによる根管充填後の根尖組織治癒
  宮治裕史; 眞弓佳代子; 金本佑生実; 西田絵利香, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 148回, 69, 69, May 2018
  (NPO)日本歯科保存学会, Japanese
• Zinc‐Fluorideガラス塗布材の細胞親和性評価
  西田絵利香; 宮治裕史; 蔀佳奈子; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 148回, 47, 47, May 2018
  (NPO)日本歯科保存学会, Japanese
• 亜鉛ガラス含有グラスアイオノマーセメントの物性および抗菌効果
  蔀佳奈子; 宮治裕史; 西田絵利香; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 148回, 48, 48, May 2018
  (NPO)日本歯科保存学会, Japanese
• 北海道大学病院歯科におけるナノシールを用いた根面う蝕進行抑制に関する臨床研究
  宮治裕史; 加藤昭人; 田中佐織, 日本口腔ケア学会雑誌, 12, 3, 281, 281, Apr. 2018
  (一社)日本口腔ケア学会, Japanese
• 過飽和液中レーザー照射によるフッ素担持アパタイトの迅速成膜
  NATHANAEL A. Joseph; 大矢根綾子; 中村真紀; 蔀佳奈子; 宮治裕史, 日本セラミックス協会年会講演予稿集(CD-ROM), 2018, ROMBUNNO.2J05, 01 Mar. 2018
  Japanese
• リゾチーム保護金クラスター・有機色素複合体の¹O₂生成能評価
  塩谷聡; 宮治裕史; 川崎英也, ナノ学会大会講演予稿集, 16th, 2018
• 酸化グラフェンスキャフォールドへの亜鉛担持による抗菌性の向上
  西田絵利香; 宮治裕史; 蔀佳奈子; 菅谷勉; 小野博信, 日本バイオマテリアル学会大会予稿集(Web), 40th, 274 (WEB ONLY), 274, 2018
  日本バイオマテリアル学会, Japanese
• Laser-Induced Forward Transfer of Fibronectin-containing Apatite Film
  奈良崎愛子; 黒崎諒三; 亀山智子; 大矢根綾子; 宮治裕史, レーザー学会研究会報告, 527th, 54, 1‐5, 5, 2018
  レーザー学会研究委員会, Japanese
• 過飽和液中レーザー照射によるフッ素担持リン酸カルシウム成膜と抗菌性評価
  NATHANAEL A. Joseph; 大矢根綾子; 中村真紀; 蔀佳奈子; 宮治裕史, 日本バイオマテリアル学会大会予稿集(Web), 39回, 80, 80, Nov. 2017
  日本バイオマテリアル学会, Japanese
• Zinc‐Fluorideガラス塗布材のイオン徐放性と抗菌効果
  西田絵利香; 宮治裕史; 蔀佳奈子; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 147回, 132, 132, Oct. 2017
  (NPO)日本歯科保存学会, Japanese
• レーザー照射による焼結水酸アパタイト基材へのフッ素担持リン酸カルシウム成膜と抗菌特性評価
  蔀佳奈子; 宮治裕史; NATHANAEL A. Joseph; 中村真紀; 大矢根綾子; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 147回, 128, 128, Oct. 2017
  (NPO)日本歯科保存学会, Japanese
• イオン徐放性S‐PRGフィラーの粒子サイズが抗菌性及び細胞親和性に及ぼす効果
  眞弓佳代子; 宮治裕史; 宮田さほり; 蔀佳奈子; 西田絵利香; 金本佑生実; 菅谷勉, 日本歯周病学会会誌(Web), 59, 128, 18 Apr. 2017
  Japanese
• 歯周治療に向けたAgクラスター/ローズベンガル複合体の特性評価
  蔀 佳奈子; 宮治 裕史; 宮田 さほり; 菅谷 勉, 日本歯周病学会会誌, 59, 春季特別, 127, 127, Apr. 2017
  (NPO)日本歯周病学会, Japanese
• イオン徐放性S-PRGフィラーの粒子サイズが抗菌性及び細胞親和性に及ぼす効果
  眞弓 佳代子; 宮治 裕史; 宮田 さほり; 蔀 佳奈子; 西田 絵利香; 金本 佑生実; 菅谷 勉, 日本歯周病学会会誌, 59, 春季特別, 128, 128, Apr. 2017
  (NPO)日本歯周病学会, Japanese
• バイオミメティック法によるリン酸カルシウム成膜スキャフォールドの特性評価
  西田絵利香; 宮治裕史; 金本佑生実; NATHANAEL A. Joseph; 中村真紀; 大矢根綾子; 村上秀輔; 眞弓佳代子; 加藤昭人; 菅谷勉, 再生医療, 16, 390, 01 Feb. 2017
  Japanese
• 歯周治療に向けたAgクラスター/ローズベンガル複合体の特性評価
  蔀佳奈子; 宮治裕史; 宮田さほり; 菅谷勉, 日本歯周病学会会誌(Web), 59, 127, 2017
  Japanese
• リゾチーム金ナノクラスター/ローズベンガル複合体の創製と光線力学的評価
  岡本一絵; 宮治裕史; 宮田さほり; 蔀佳奈子; 川崎英也; 赤坂司; 菅谷勉, 日本バイオマテリアル学会大会予稿集(Web), 39th, 132, 132, 2017
  日本バイオマテリアル学会, Japanese
• バイオミメティック法によるリン酸カルシウム成膜スキャフォールドの生物学的特性
  金本佑生実; 宮治裕史; 西田絵利香; NATHANAEL A. Joseph; 中村真紀; 大矢根綾子; 村上秀輔; 眞弓佳代子; 加藤昭人; 菅谷勉, 日本バイオマテリアル学会シンポジウム予稿集, シンポジウム2016, 118, 118, Nov. 2016
  日本バイオマテリアル学会, Japanese
• Agクラスター/ローズベンガル複合体の創製と光励起による抗菌性の発現
  蔀佳奈子; 宮治裕史; 宮田さほり; 佐伯歩; 川崎英也; 長谷川裕美; 岩永敏彦; 菅谷勉, 日本バイオマテリアル学会シンポジウム予稿集, シンポジウム2016, 141, 141, Nov. 2016
  日本バイオマテリアル学会, Japanese
• レーザー光を用いて作製した銀ナノ粒子含有リン酸カルシウム球状粒子の抗菌性評価
  中村真紀; 大矢根綾子; 宮田さほり; 佐伯歩; 宮治裕史, 日本バイオマテリアル学会シンポジウム予稿集, シンポジウム2016, 248, 248, Nov. 2016
  日本バイオマテリアル学会, Japanese
• イオン徐放性S‐PRGナノフィラーを用いた歯面の機能性コーティング
  眞弓佳代子; 宮治裕史; 宮田さほり; 蔀佳奈子; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 145回, 156, 156, Oct. 2016
  (NPO)日本歯科保存学会, Japanese
• レーザー援用バイオミメティック法によるレジン表面へのリン酸カルシウムの析出
  西田絵利香; 宮治裕史; NATHANAEL A.Joseph; 中村真紀; 大矢根綾子; 田中佐織; 田中佐織; 田中享; 加藤昭人; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 145回, 166, 166, Oct. 2016
  (NPO)日本歯科保存学会, Japanese
• 酸化グラフェンと近赤外線光を用いた光熱的抗菌治療法の開発
  長尾敬志; 宮治裕史; 西田絵利香; 加藤昭人; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 145回, 160, 160, Oct. 2016
  (NPO)日本歯科保存学会, Japanese
• 液中レーザー溶融法を用いて合成した銀含有リン酸カルシウム球状ナノ粒子の抗菌性評価
  宮田さほり; 宮治裕史; 中村真紀; 大矢根綾子; 菅谷勉, 日本歯周病学会会誌(Web), 58, 121, 09 Sep. 2016
  Japanese
• 酸化グラフェンスキャフォールド埋植による歯周組織治癒促進効果
  川本 康平; 宮治 裕史; 加藤 昭人; 西田 絵利香; 小川 幸佑; 舘山 彰人; 降旗 友和; 宮田 さほり; 蔀 佳奈子; 菅谷 勉, 日本歯周病学会会誌, 58, 秋季特別, 110, 110, Sep. 2016
  (NPO)日本歯周病学会, Japanese
• 液中レーザー溶融法を用いて合成した銀含有リン酸カルシウム球状ナノ粒子の抗菌性評価
  宮田 さほり; 宮治 裕史; 中村 真紀; 大矢根 綾子; 菅谷 勉, 日本歯周病学会会誌, 58, 秋季特別, 121, 121, Sep. 2016
  (NPO)日本歯周病学会, Japanese
• ナノシールによる根面う蝕の進行抑制に関する研究
  宮治裕史; 田中佐織; 加藤昭人; 井上加菜; 西尾啓英; 川浪雅光, 日本歯科医師会雑誌, 69, 5, 455, 455, Aug. 2016
  (公社)日本歯科医師会, Japanese
• 酸化グラフェンでコーティングされた歯根象牙質の抗菌性の評価
  長尾敬志; 宮治裕史; 西田絵利香; 川浪雅光, 日本歯周病学会会誌(Web), 58, 126, 18 Apr. 2016
  Japanese
• 酸化グラフェン配合スキャフォールドによる骨形成効果
  降籏友和; 宮治裕史; 西田絵利香; 川本康平; 小川幸佑; 百瀬赳人; 館山彰人; 長尾敬志; 吉田崇; 加藤昭人; 川浪雅光, 日本歯周病学会会誌(Web), 58, 139, 18 Apr. 2016
  Japanese
• 酸化グラフェンでコーティングされた歯根象牙質の抗菌性の評価
  長尾 敬志; 宮治 裕史; 西田 絵利香; 川浪 雅光, 日本歯周病学会会誌, 58, 春季特別, 126, 126, Apr. 2016
  (NPO)日本歯周病学会, Japanese
• 金クラスターを用いた抗菌的光線力学療法の開発 LED光源の違いによる検討
  宮田 さほり; 宮治 裕史; 蔀 佳奈子; 川浪 雅光, 日本歯周病学会会誌, 58, 春季特別, 126, 126, Apr. 2016
  (NPO)日本歯周病学会, Japanese
• 酸化グラフェン配合スキャフォールドによる骨形成効果
  降籏 友和; 宮治 裕史; 西田 絵利香; 川本 康平; 小川 幸佑; 百瀬 赳人; 館山 彰人; 長尾 敬志; 吉田 崇; 加藤 昭人; 川浪 雅光, 日本歯周病学会会誌, 58, 春季特別, 139, 139, Apr. 2016
  (NPO)日本歯周病学会, Japanese
• 歯表面での3D微細構造構築によるバイオミメティック次世代歯科治療へ向けて
  赤坂司; 加我公行; 宮治裕史; 横山敦郎; 吉田靖弘, 日本歯科医学会誌, 35, 36, 36, Mar. 2016
  日本歯科医学会, Japanese
• チタンと生体界面間へのカーボンナノチューブによるナノ構造の応用
  西田絵利香; 宮治裕史; 梅田純子; 近藤勝義; 滝田裕子; 吉田崇; 村上秀輔; 眞弓佳代子; 蔀佳奈子; 古月文志; 川浪雅光, 再生医療, 15, 359, 01 Feb. 2016
  Japanese
• TCPナノ粒子を用いた組織再生用ナノ材料の創製と応用
  宮治裕史; 古月文志; 加藤昭人; 小川幸佑; 村上秀輔; 西田絵利香; 宮田さほり; 川本康平; 滝田裕子; 岩永敏彦; 本郷裕美; 網塚憲生; 川浪雅光, 再生医療, 15, 297, 01 Feb. 2016
  Japanese
• 多孔質体を形成するエマルション骨ペーストの開発
  加藤昭人; 宮治裕史; 舘山彰人; 西田絵利香; 岩崎泰彦; 藤井秀司; 滝田裕子; 吉田崇; 小川幸佑; 百瀬赳人; 村上秀輔; 長尾敬志; 川浪雅光, 再生医療, 15, 305, 01 Feb. 2016
  Japanese
• 金クラスターを用いた抗菌的光線力学療法の開発―LED光源の違いによる検討―
  宮田さほり; 宮治裕史; 蔀佳奈子; 川浪雅光, 日本歯周病学会会誌(Web), 58, 126, 2016
  Japanese
• 酸化グラフェンスキャフォールド埋植による歯周組織治癒促進効果
  川本康平; 宮治裕史; 加藤昭人; 西田絵利香; 小川幸佑; 舘山彰人; 降旗友和; 宮田さほり; 蔀佳奈子; 菅谷勉, 日本歯周病学会会誌(Web), 58, 110, 2016
  Japanese
• 金クラスターを用いた抗菌的光線力学療法の開発―歯周病原菌に対する抗菌効果と細胞親和性―
  宮田さほり; 宮治裕史; 佐伯歩; 川崎英也; 田中佐織; 田中佐織; 菅谷勉, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 145th, 148, 148, 2016
  (NPO)日本歯科保存学会, Japanese
• ゲニピン添加マイクロ・ナノパターン化ゼラチン上での細胞接着
  蒔田玲果; 赤坂司; 宮治裕史; 宮田さほり; 菅谷勉, 日本バイオマテリアル学会シンポジウム予稿集, 2016, 142, 142, 2016
  日本バイオマテリアル学会, Japanese
• TCPナノ粒子配合スキャフォールドのバイオアクティブ効果と組織再生促進
  宮治 裕史; 川浪 雅光, 日本再生歯科医学会誌, 13, 1, 57, 57, Dec. 2015
  日本再生歯科医学会, Japanese
• 組織再生用ナノ酸化グラフェンスキャフォールドによる骨新生効果
  西田絵利香; 宮治裕史; 滝田裕子; 岩永敏彦; 加藤昭人; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 143回, 200, 200, Nov. 2015
  (NPO)日本歯科保存学会, Japanese
• S‐PRGフィラーの根管充填用シーラーへの応用と生物学的機能評価
  眞弓佳代子; 宮治裕史; 宮田さほり; 村上秀輔; 西田絵利香; 蔀佳奈子; 田中佐織; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 143回, 50, 50, Nov. 2015
  (NPO)日本歯科保存学会, Japanese
• 根面う蝕へのナノシール塗布によるダイアグノデント測定値の変動
  加藤昭人; 宮治裕史; 田中佐織; 井上加菜; 西尾啓英; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 143回, 100, 100, Nov. 2015
  (NPO)日本歯科保存学会, Japanese
• 歯科応用を目指した抗菌性リン酸カルシウム球状粒子の作製
  中村真紀; 大矢根綾子; 清水禎樹; 宮治裕史, 日本バイオマテリアル学会大会予稿集, 37回, 122, 122, Nov. 2015
  日本バイオマテリアル学会, Japanese
• リン酸カルシウムナノ粒子含有量の違いがスキャフォールドの特性に及ぼす効果
  村上秀輔; 宮治裕史; 西田絵利香; 川本康平; 宮田さほり; 滝田裕子; 岩永敏彦; 古月文志; 川浪雅光, 日本バイオマテリアル学会大会予稿集, 37回, 275, 275, Nov. 2015
  日本バイオマテリアル学会, Japanese
• 酸化グラフェン配合スキャフォールドによる骨増生促進効果
  川本康平; 宮治裕史; 吉田崇; 西田絵利香; 加藤昭人; 舘山彰人; 滝田裕子; 岩永敏彦; 川浪雅光, 日本バイオマテリアル学会大会予稿集, 37回, 205, 205, Nov. 2015
  日本バイオマテリアル学会, Japanese
• GlcNAc架橋ゼラチン・キトサンスキャフォールドとFGF‐2による骨新生効果
  吉田崇; 宮治裕史; 西田絵利香; 加藤昭人; チャオチャイ ティティラット; 古池哲也; 田村裕; 川浪雅光, 日本バイオマテリアル学会大会予稿集, 37回, 342, 342, Nov. 2015
  日本バイオマテリアル学会, Japanese
• 金クラスターとLED光照射器による光殺菌歯科治療法の開発
  宮田さほり; 宮治裕史; 西田絵利香; 川崎英也; 山本真希; 滝田裕子; 牛島夏未; 岩永俊彦; 赤坂司; 田中佐織; 川浪雅光, 日本バイオマテリアル学会大会予稿集, 37回, 183, 183, Nov. 2015
  日本バイオマテリアル学会, Japanese
• 銀クラスターのS.mutansに対する抗菌性の評価
  蔀佳奈子; 宮治裕史; 宮田さほり; 眞弓佳代子; 川崎英也; 長谷川裕美; 富永千明; 岩永俊彦; 川浪雅光, 日本バイオマテリアル学会大会予稿集, 37回, 193, 193, Nov. 2015
  日本バイオマテリアル学会, Japanese
• 酸化グラフェン(GO)スキャフォールドの生体応用
  西田絵利香; 宮治裕史; 金山和泉; 川浪雅光; 滝田裕子; 辻妹井子, 北海道外科雑誌, 60, 1, 96, 96, Jun. 2015
  北海道外科学会, Japanese
• 金クラスターの光励起による細菌増殖抑制
  宮田さほり; 宮治裕史; 田中佐織; 西田絵利香; 川浪雅光, 日本歯周病学会会誌(Web), 57, 113, 08 Apr. 2015
  Japanese
• 金クラスターの光励起による細菌増殖抑制
  宮田 さほり; 宮治 裕史; 田中 佐織; 西田 絵利香; 川浪 雅光, 日本歯周病学会会誌, 57, 春季特別, 113, 113, Apr. 2015
  (NPO)日本歯周病学会, Japanese
• TCPナノ粒子配合スキャフォールドのバイオアクティブ効果と組織再生促進
  宮治裕史; 川浪雅光, 日本再生歯科医学会学術大会および総会プログラム・抄録集, 13th, 49, 2015
  Japanese
• α‐TCP/PLGA微粒子エマルション骨ペーストの生体適合性評価
  舘山彰人; 宮治裕史; 加藤昭人; 西田絵利香; 岩崎泰彦; 藤井秀司; 滝田裕子; 吉田崇; 小川幸佑; 百瀬赳人; 村上秀輔; 長尾敬志; 川浪雅光, 日本バイオマテリアル学会大会予稿集, 36回, 249, 249, Nov. 2014
  日本バイオマテリアル学会, Japanese
• 金クラスターの歯科用光照射器による光励起が培養細胞および細菌へ及ぼす影響
  西田絵利香; 宮治裕史; 川崎英也; 山本真希; 宮田さほり; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 141回, 234, 234, Oct. 2014
  (NPO)日本歯科保存学会, Japanese
• イオン徐放性S‐PRGフィラー含有根管シーラーの組織学的検討
  田中佐織; 宮治裕史; 西田絵利香; 井上加菜; 宮田さほり; 川本康平; 村上秀輔; 金山和泉; 加藤昭人; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 141回, 127, 127, Oct. 2014
  (NPO)日本歯科保存学会, Japanese
• コンポジットレジン表面へのマイクロ・ナノ3次元微細構造の付与
  宮治裕史; 西田絵利香; 今村琢也; 長尾敬志; 田中佐織; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 140回, 172, 172, Jun. 2014
  (NPO)日本歯科保存学会, Japanese
• ナノβ‐TCP/コラーゲンスキャフォールドの抜歯窩治癒促進効果
  加藤昭人; 宮治裕史; 光銭裕太; 小川幸佑; 百瀬赴人; 西田絵利香; 村上秀輔; 井原朝子; 吉田崇; 田中佐織; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 140回, 171, 171, Jun. 2014
  (NPO)日本歯科保存学会, Japanese
• FGF2含有コラーゲンハイドロゲルスキャフォールドの根分岐部II級骨欠損への応用
  百瀬赳人; 宮治裕史; 加藤昭人; 光銭裕太; 小川幸佑; 井原朝子; 吉田崇; 村上秀輔; 西田絵利香; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 56, 春季特別, 105, 105, Apr. 2014
  (NPO)日本歯周病学会, Japanese
• ナノβ‐TCPコーティングスキャフォールドとFGF2併用による歯周組織治癒
  小川幸佑; 宮治裕史; 加藤昭人; 光銭裕太; 百瀬赳人; 吉田崇; 西田絵利香; 村上秀輔; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 56, 春季特別, 105, 105, Apr. 2014
  (NPO)日本歯周病学会, Japanese
• マイクロ・ナノパターンを付与したコンポジットレジン上への細胞接着
  今村琢也; 宮治裕史; 赤坂司; 西田絵利香; 長尾敬志; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 56, 春季特別, 120, 120, Apr. 2014
  (NPO)日本歯周病学会, Japanese
• チタン表面のカーボンナノチューブコーティングと生体適合性評価
  西田絵利香; 宮治裕史; 滝田裕子; 梅田純子; 近藤勝義; 古月文志; 田中佐織; 加藤昭人; 金山和泉; 川浪雅光, 再生医療, 13, 352, 27 Jan. 2014
  Japanese
• 酸化グラフェンコーティングした組織再生用スキャフォールドの特性比較
  宮治裕史; 西田絵利香; 滝田裕子; 金山和泉; 辻妹井子; 川浪雅光, 再生医療, 13, 351, 27 Jan. 2014
  Japanese
• カーボンナノチューブとナノリン酸カルシウムによるスキャフォールドコーティング
  村上秀輔; 宮治裕史; 西田絵利香; 滝田裕子; 吉田崇; 古月文志; 川浪雅光, 日本バイオマテリアル学会大会予稿集, 35回, 464, 464, Nov. 2013
  日本バイオマテリアル学会, Japanese
• キトサンを用いたマイクロパターン化フィルムの作製
  西田絵利香; 宮治裕史; 赤坂司; 川浪雅光, 日本バイオマテリアル学会大会予稿集, 35回, 459, 459, Nov. 2013
  日本バイオマテリアル学会, Japanese
• 北海道大学病院における垂直歯根破折の実態調査
  中塚愛; 菅谷勉; 元木洋史; 井上加菜; 田中佐織; 宮治裕史; 川浪雅光; 坂上竜資, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 139回, 132, 132, Oct. 2013
  (NPO)日本歯科保存学会, Japanese
• 酸化グラフェンおよびグラフェンの歯周病治療用デバイスとしての可能性
  西田絵利香; 宮治裕史; 金山和泉; 田中佐織; 川浪雅光; 辻妹井子; 滝田裕子, 北海道外科雑誌, 58, 1, 88, 89, Jun. 2013
  北海道外科学会, Japanese
• 異なる濃度のナノβ‐TCP分散液でコーティングしたコラーゲンスキャフォールドの特性評価
  宮治裕史; 西田絵利香; 村上秀輔; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 138回, 228, 228, May 2013
  (NPO)日本歯科保存学会, Japanese
• グラフェンおよび酸化グラフェンフィルムに対する生体反応
  金山和泉; 宮治裕史; 西田絵利香; 辻妹井子; 井上加菜; 井原朝子; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 138回, 76, 76, May 2013
  (NPO)日本歯科保存学会, Japanese
• 新規イオン徐放性S‐PRGフィラー含有根管シーラーの生体適合性の検討
  田中佐織; 宮治裕史; 西田絵利香; 金山和泉; 加藤昭人; 中塚稔之; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 138回, 208, 208, May 2013
  (NPO)日本歯科保存学会, Japanese
• ナノβ‐TCP/コラーゲンスキャフォールドのイヌ歯周組織欠損モデルへの応用
  小川幸佑; 宮治裕史; 加藤昭人; 光銭裕太; 吉田崇; 百瀬赳人; 西田絵利香; 村上秀輔; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 55, 春季特別, 111, 111, Apr. 2013
  (NPO)日本歯周病学会, Japanese
• 酸化グラフェンおよびグラフェンによる歯根象牙質コーティングとタンパク質吸着性の評価
  西田絵利香; 宮治裕史; 辻妹井子; 田中佐織; 金山和泉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 55, 春季特別, 115, 115, Apr. 2013
  (NPO)日本歯周病学会, Japanese
• 高気孔性PLGA/β‐TCPスキャフォールドの骨増生効果
  吉田崇; 宮治裕史; 大谷香織; 井上加奈; 井原朝子; 小川幸佑; 島田歩; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 55, 春季特別, 91, 91, Apr. 2013
  (NPO)日本歯周病学会, Japanese
• FGF含有コラーゲンハイドロゲルスキャフォールドの根分岐部class II骨欠損への応用
  百瀬赳人; 宮治裕史; 加藤昭人; 光銭裕太; 小川幸佑; 井原朝子; 吉田崇; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 55, 春季特別, 112, 112, Apr. 2013
  (NPO)日本歯周病学会, Japanese
• グラフェンと酸化グラフェンが培養骨芽細胞に及ぼす影響
  金山和泉; 宮治裕史; 滝田裕子; 辻妹井子; 井上加菜; 井原朝子; 西田絵利香; 川浪雅光, 日本バイオマテリアル学会シンポジウム予稿集, シンポジウム2012, 272, 272, Nov. 2012
  日本バイオマテリアル学会, Japanese
• プラスチックコーティングした高気孔性β‐TCPスキャフォールドの物性と生体親和性の評価
  吉田崇; 宮治裕史; 中根和靖; 井原朝子; 井上加菜; 小川幸佑; 島田歩; 大谷香織; 川浪雅光, 日本バイオマテリアル学会シンポジウム予稿集, シンポジウム2012, 195, 195, Nov. 2012
  日本バイオマテリアル学会, Japanese
• カーボンナノチューブコーティングチタンの生体応用
  宮治裕史; 西田絵利香; 田中佐織; 梅田純子; 近藤勝義; 古月文志; 金山和泉; 加藤昭人; 川浪雅光, 日本バイオマテリアル学会シンポジウム予稿集, シンポジウム2012, 244, 244, Nov. 2012
  日本バイオマテリアル学会, Japanese
• ナノβ‐TCP/コラーゲンスキャフォールドの作製と骨再生療法への応用
  井原朝子; 宮治裕史; 古月文志; 井上加菜; 光銭裕太; 金山和泉; 吉田崇; 菅谷勉; 田中佐織; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 137回, 46, 46, Oct. 2012
  (NPO)日本歯科保存学会, Japanese
• グラフェンおよび酸化グラフェンによる象牙質コーティング法の検討
  西田絵利香; 宮治裕史; 辻妹井子; 田中佐織; 金山和泉; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 137回, 153, 153, Oct. 2012
  (NPO)日本歯科保存学会, Japanese
• コンポジットレジンへのカーボンナノチューブコーティング技術の応用
  田中佐織; 宮治裕史; 西田絵利香; 古月文志; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 137回, 165, 165, Oct. 2012
  (NPO)日本歯科保存学会, Japanese
• 高気孔性β‐TCPスキャフォールドの作製と評価
  宮治裕史; 吉田崇; 光銭裕太; 大谷香織; 井上加菜; 井原朝子; 金山和泉; 加藤昭人; 田中佐織; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 54, 春季特別, 144, 144, Apr. 2012
  (NPO)日本歯周病学会, Japanese
• 高気孔性β‐TCPフォームへのFGF2添加による骨新生効果
  宮治裕史; 大谷香織; 西村浩之; 井上加菜; 井原朝子; 吉田崇; 小川幸佑; 田中佐織; 川浪雅光, 日本バイオマテリアル学会大会予稿集, 33回, 461, 461, Nov. 2011
  日本バイオマテリアル学会, Japanese
• ナノβ‐TCP配合コラーゲンスキャフォールドの作製と評価
  井原朝子; 宮治裕史; 古月文志; 野田坂佳伸; 大谷香織; 井上加菜; 光銭裕太; 金山和泉; 吉田崇; 田中佐織; 菅谷勉; 川浪雅光, 日本バイオマテリアル学会大会予稿集, 33回, 173, 173, Nov. 2011
  日本バイオマテリアル学会, Japanese
• 新規デンタルユニット連動型自律神経活動モニターシステムの開発
  下地伸司; 菅谷勉; 根岸淳; 田中佐織; 伊藤豊; 小田島朝臣; 宮治裕史; 齋藤恵美子; 川村直人; 元木洋史; 臼井薫; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 135回, 208, 208, Sep. 2011
  (NPO)日本歯科保存学会, Japanese
• bFGF製剤を用いた歯周組織再生療法および北大における臨床成績
  宮治裕史, 日本臨床歯周病学会会誌, 29, 1, 76, 76, Aug. 2011
  (NPO)日本臨床歯周病学会, Japanese
• ゼラチンコーティングβ‐TCPフォームの開発と骨再生療法への応用
  宮治裕史; 横山裕之; 西村浩之; 光銭裕太; 井原朝子; 大谷香織; 金山和泉; 田中佐織; 川浪雅光, 日本バイオマテリアル学会大会予稿集, 32回, 331, 331, Nov. 2010
  日本バイオマテリアル学会, Japanese
• 骨整形せずに垂直性骨欠損が回復した限局型重度慢性歯周炎の1症例
  石塚良介; 宮治裕史; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 52, 秋季特別, 119, 119, Sep. 2010
  (NPO)日本歯周病学会, Japanese
• BMPの根面塗布とコラーゲンハイドロゲル‐スポンジ複合体の併用による歯周組織再生
  加藤昭人; 宮治裕史; 石塚良介; 得永佳介; 井上加菜; 光銭裕太; 横山裕之; 田中佐織; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 52, 秋季特別, 93, 93, Sep. 2010
  (NPO)日本歯周病学会, Japanese
• FGF2/コラーゲンハイドロゲルの骨再生スキャフォールドへの応用
  宮治裕史; 小林尚子; 菅谷勉; 井原朝子; 鷲巣太郎; 横山裕之; 光銭裕太; 川浪雅光, 北海道外科雑誌, 55, 1, 85, 85, Jun. 2010
  北海道外科学会, Japanese
• 1壁性骨欠損におけるコラーゲンハイドロゲル‐スポンジ複合体による歯周組織再生
  加藤昭人; 宮治裕史; 光銭裕太; 横山裕之; 石塚良介; 得永佳介; 井上加菜; 逸見優; 田中佐織; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 53rd, 0, 113, 87, 10 Apr. 2010
  JAPANESE SOCIETY OF PERIODONTOLOGY, Japanese
• 根分岐部classII骨欠損におけるコラーゲンハイドロゲル‐スポンジ複合体による歯周組織再生
  光銭裕太; 宮治裕史; 加藤昭人; 横山裕之; 逸見優; 小林尚子; 石塚良介; 田中佐織; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 53rd, 0, 96, 35, 10 Apr. 2010
  JAPANESE SOCIETY OF PERIODONTOLOGY, Japanese
• 根分岐部class II骨欠損にコラーゲンハイドロゲル‐スポンジ複合体を移植した場合の組織反応
  光銭裕太; 宮治裕史; 加藤昭人; 横山裕之; 逸見優; 小林尚子; 石塚良介; 田中佐織; 菅谷勉; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 131回, 244, 244, Oct. 2009
  (NPO)日本歯科保存学会, Japanese
• 培養骨髄細胞シートとBMP含有FC‐HACスポンジの併用移植による異所性骨形成
  井上加菜; 宮治裕史; 福田寛恵; 横山裕之; 田中佐織; 菅谷勉; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 131回, 62, 62, Oct. 2009
  (NPO)日本歯科保存学会, Japanese
• FGF‐2含有コラーゲンハイドロゲル‐スポンジ複合体が骨新生に与える効果
  小林尚子; 宮治裕史; 井原朝子; 鷲巣太郎; 横山裕之; 光銭裕太; 菅谷勉; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集(Web), 131回, 51, 51, Oct. 2009
  (NPO)日本歯科保存学会, Japanese
• コラーゲンハイドロゲル‐スポンジ複合体を頭蓋骨に移植した場合のスキャフォールドとしての有効性とBMP併用による骨増生
  得永佳介; 宮治裕史; 石塚良介; 加藤昭人; 井上加菜; 逸見優; 光銭裕太; 玉川博貴; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 51, 春季特別, 110, 110, Apr. 2009
  (NPO)日本歯周病学会, Japanese
• 4‐META/MMA‐TBBレジンの硬化条件と組織反応
  川村直人; 菅谷勉; 元木洋史; 宮治裕史; 川浪雅光, 北海道外科雑誌, 53, 1, 123, 123, Jun. 2008
  北海道外科学会, Japanese
• 象牙質表面へのBMP‐2とコラーゲンハイドロゲルの塗布による歯槽骨再生と骨性癒着
  石塚良介; 宮治裕史; 加藤昭人; 得永佳介; 井上加菜; 田中陽; 菅谷勉; 川浪雅光, 再生医療, 7, 268, 22 Feb. 2008
  Japanese
• 1壁性骨欠損においてBMP‐2の根面塗布は歯周組織再生を高める
  加藤昭人; 宮治裕史; 石塚良介; 得永佳介; 井上加菜; 菅谷勉; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集, 50, 秋季特別, 47, 47, Oct. 2007
  (NPO)日本歯科保存学会, Japanese
• 当科における造血幹細胞移植患者の歯周治療
  田中佐織; 菅谷勉; 松本敦至; 小田島朝臣; 宮治裕史; 下地伸司; 中山絵里; 川浪雅光, 日本歯科保存学会学術大会プログラムおよび講演抄録集, 50, 秋季特別, 207, 207, Oct. 2007
  (NPO)日本歯科保存学会, Japanese
• BMP‐2処理象牙質面における硬組織形成と象牙質吸収のビスフォスフォネート投与による抑制
  本郷哲也; 宮治裕史; 川村直人; 天雲太一; 大谷香織; 得永佳介; 菅谷勉; 川浪雅光, 再生医療, 6, 279, 19 Feb. 2007
  Japanese
• Bisphosphonate投与はBMP‐2処理した象牙質表面へのセメント質様硬組織形成と象牙質吸収を抑制する
  本郷哲也; 宮治裕史; 川村直人; 天雲太一; 大谷香織; 得永佳介; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 48, 秋季特別, 149, 149, Sep. 2006
  (NPO)日本歯周病学会, Japanese
• 歯根象牙質表面へのBMP‐2とコラーゲンハイドロゲルの塗布が歯周組織再生を高める
  石塚良介; 宮治裕史; 伊部敬介; 加藤昭人; 得永佳介; 井上加菜; 田中陽; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 48, 秋季特別, 148, 148, Sep. 2006
  (NPO)日本歯周病学会, Japanese
• 象牙質表面へのrhBMP‐2塗布によるセメント質の再生
  菅谷勉; 宮治裕史; 川浪雅光, 北海道外科雑誌, 51, 1, 79, 79, Jun. 2006
  北海道外科学会, Japanese
• 歯根象牙質へのBMP‐2塗布による歯周組織再生の経時的評価
  得永佳介; 宮治裕史; 伊部敬介; 石塚良介; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 48, 春季特別, 107, 107, Mar. 2006
  (NPO)日本歯周病学会, Japanese
• Q&A 歯周 BMPの効果的術式
  宮治 裕史; 川浪 雅光, DENTAL DIAMOND, 30, 15, 102, 103, Nov. 2005
  (株)デンタルダイヤモンド社, Japanese
• 骨髄穿孔とコラーゲンスポンジの骨上への埋植が骨増生に及ぼす効果
  下地 伸司; 宮治 裕史; 辻 ひふみ; 本郷 哲也; 中塚 愛; 菅谷 勉; 川浪 雅光, 日本歯科保存学雑誌, 48, 秋季特別, 65, 65, Oct. 2005
  (NPO)日本歯科保存学会, Japanese
• Effect of transplantation of collagen sponge with bone marrow on bony augmentation of rat femora
  TSUJI Hifumi; MIYAJI Hirofumi; SHIMOJI Shinji; FUKUSHIMA Chifumi; SUGAYA Tsutomu; KAWANAMI Masamitsu, 日本歯科保存学雑誌, 48, 秋季特別, 66, 66, Oct. 2005
  (NPO)日本歯科保存学会, Japanese
• 象牙質表面処理剤の違いがBMP‐2による硬組織形成と象牙質吸収に及ぼす影響
  天雲太一; 宮治裕史; 川村直人; 伊部敬介; 本郷哲也; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 47, 秋季特別, 78, 78, Aug. 2005
  (NPO)日本歯周病学会, Japanese
• 骨髄間質細胞とBMP含有コラーゲンスポンジの併用移植による骨新生
  宮治裕史; 本郷哲也; 天雲太一; 福島千史; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 47, 秋季特別, 101, 101, Aug. 2005
  (NPO)日本歯周病学会, Japanese
• BMPを用いた歯周組織再生療法
  川浪 雅光; 齋藤 彰; 松本 敦至; 小田島 朝臣; 山路 公造; 宮治 裕史; 加藤 煕, 歯界展望, 特別号, 健康な心と身体は口腔から-発ヨコハマ2004-, 247, 247, Jun. 2005
  医歯薬出版(株), Japanese
• 象牙質表面のBMP-2処理によるセメント質再生
  菅谷 勉; 宮治 裕史; 川浪 雅光, 日本再生歯科医学会誌, 2, 1, 72, 72, Sep. 2004
  日本再生歯科医学会, Japanese
• 歯根象牙質表面へのBMP‐2の塗布が歯周組織再生を高める
  伊部敬介; 宮治裕史; 加藤勝也; 石塚良介; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 46, 秋季特別, 90, 90, Sep. 2004
  (NPO)日本歯周病学会, Japanese
• BMPを用いた歯周組織再生療法
  川浪 雅光; 斎藤 彰; 松本 敦至; 小田島 朝臣; 山路 公造; 宮治 裕史; 加藤 熈, 日本歯科医師会雑誌, 57, 4, 386, 386, Jul. 2004
  (公社)日本歯科医師会, Japanese
• Dentin resorption and hard-tissue formation following implantation of BMP-2-applied dentin.
  H. Miyaji; T. Sugaya; K. Kato; N. Kawamura; H. Tsuji; M. Kawanami, JOURNAL OF DENTAL RESEARCH, 82, B146, B146, Jun. 2003
  English, Summary international conference
• Prognosis of periradicular surgery using 4-META/MMA-TBB resin as root-end sealant.
  T. Sugaya; H. Noguchi; H. Miyaji; M. Kawanami, JOURNAL OF DENTAL RESEARCH, 82, B117, B117, Jun. 2003
  English, Summary international conference
• BMP‐2で処理した象牙質表面における象牙質吸収と硬組織形成
  宮治裕史; 菅谷勉; 加藤勝也; 川村直人; 川浪雅光, 日本歯科保存学会プログラムおよび講演抄録集, 117th, 118, Nov. 2002
  Japanese
• BMP-2で処理した象牙質表面における象牙質吸収と硬組織形成
  宮治 裕史; 菅谷 勉; 加藤 勝也; 川村 直人; 川浪 雅光, 日本歯科保存学雑誌, 45, 秋季特別, 117, 117, Oct. 2002
  (NPO)日本歯科保存学会, Japanese
• アテロコラーゲンスポンジの組成と濃度が人工的骨欠損の治癒に及ぼす影響
  加藤勝也; 宮治裕史; 川村直人; 伊部敬介; 菅谷勉; 川浪雅光, 日本歯科保存学会プログラムおよび講演抄録集, 45, 秋季特別, 67, 67, Oct. 2002
  (NPO)日本歯科保存学会, Japanese
• コラーゲンゲル生体接着剤を応用した歯肉剥離掻は手術後の歯周組織再生
  加藤勝也; 宮治裕史; 川村直人; 菅谷勉; 川浪雅光, 日本歯周病学会学術大会プログラムおよび講演抄録集, 45th, 134, 25 Mar. 2002
  Japanese
• Periodontal tissue regeneration after flap operation using collagen-gel tissue adhesive
  KATO Katsuya; MIYAJI Hirofumi; KAWAMURA Naoto; SUGAYA Tsutomu; KAWANAMI Masamitsu, Journal of the Japanese Association of Periodontology, 44, 春季特別, 134, 134, Mar. 2002
  The Japanese Society of Periodontology, Japanese
• rhBMP‐2で処理した象牙質が歯肉結合組織の硬組織形成能に及ぼす効果
  宮治裕史, 北海道歯学雑誌, 21, 2, 423, 423, Dec. 2000
  北海道歯学会, Japanese
• rhBMP‐2で処理した象牙質を歯肉結合組織に移植した場合の硬組織形成
  宮治裕史; 菅谷勉; 宮本哲朗; 加藤勝也; 加藤ひろし, 日本歯周病学会学術大会プログラムおよび講演抄録集, 42, 秋季特別, 150, 150, Sep. 2000
  (NPO)日本歯周病学会, Japanese
• rhBMP‐2で処理したEDTA脱灰象牙質が歯肉線維芽細胞のALP活性に及ぼす影響
  宮治裕史; 菅谷勉; 加藤ひろし, 日本歯周病学会学術大会プログラムおよび講演抄録集, 42, 春季特別, 124, 124, Apr. 2000
  (NPO)日本歯周病学会, Japanese
• 再植歯の人工的歯根膜欠損が治癒に及ぼす影響 とくに初期の組織変化について
  二宮昭; 本郷興人; 宮治裕史; 加藤ひろし, 日本歯周病学会学術大会プログラムおよび講演抄録集, 42, 春季特別, 100, 100, Apr. 2000
  (NPO)日本歯周病学会, Japanese
• スーパーボンドによる逆根充直後に血液が接触した場合の表面性状
  野口裕史; 菅谷勉; 田中佐織; 宮治裕史; 加藤ひろし, 日本歯科保存学会プログラムおよび講演抄録集, 43, 春季特別, 97, 97, Mar. 2000
  (NPO)日本歯科保存学会, Japanese
• rhBMP‐2で処理した象牙質が歯肉結合組織の硬組織形成能に及ぼす効果
  宮治裕史, 北海道歯学会学術大会プログラム講演内容抄録, 2000, 24, 2000
  Japanese
• ルートプレーニングと酸処理した根面にrhBMP‐2を応用した場合の歯周組織の反応
  岩並知敏; 伊藤豊; 北郷興人; 二宮昭; 宮治裕史; 山本俊樹; 木村喜芳; 加藤ひろし, 日本歯周病学会学術大会プログラムおよび講演抄録集, 41, 春季特別, 82, 82, Mar. 1999
  (NPO)日本歯周病学会, Japanese
• 歯の再植時における歯根膜損傷部の再生に関する研究
  二宮 昭; 岩並 知敏; 本郷 興人; 伊藤 豊; 宮治 裕史; 山本 俊樹; 木村 喜芳; 加藤 熈, 日本歯周病学会会誌, 40, 秋季特別, 125, 125, Sep. 1998
  (NPO)日本歯周病学会, Japanese

• Development and market of scaffolds for tissue engineering and regenerative medicine
  MIYAJI Hirofumi, 第１編技術・開発編，第13章ナノカーボンスキャフォールド．再生医療用足場材料の開発と市場
  シーエムシー出版, Mar. 2016, [Joint work]
• Advances in Oral Tissue Engineering.
  H. Miyaji; M Kawanami, Chapter 4, Dentin conditioning with BMP for reconstruction of periodontal attachment.
  Quintessence Publishing Co, Inc., Jun. 2014, [Joint work]
• カーボンナノチューブ応用最前線
  宮治 裕史, 第10章生体材料関連，1，CNTで表面改変したバイオマテリアルの生体応用
  シーエムシー出版, 2014, [Joint work]
• 歯周病と全身の健康を考える
  宮治裕史; 川浪雅光, メインテナンス
  財団法人ライオン歯科衛生研究所, 2007, [Joint work]

• Bioactive Coating by Laser-Induced Forward Transfer for Dental Applications
  A Narazaki; M Nakamura; A Oyane; R Kurosaki; T Kameyama; H Miyaji
  CBM 2025
  08 Sep. 2025 - 12 Sep. 2025
• In situ artificial enamel formation via laser-assisted rapid pseudo-biomineralization
  A Oyane; K Onuma; M Nakamura; S Tanaka; H Miyaji
  WBC 2024, May 2024
• Laser-induced forward transfer of bioactive film and ink
  A Narazaki; M Nakamura; A Oyane; H Miyaji
  OPL 2023, Dec. 2023
  [Invited]
• Novel Gold nanocluster-based Photosensitizers for Antimicrobial Photodynamic Therapy
  H Kawasaki; I Okamoto; H Miyaji
  ICPACKK 2022, Nov. 2022, English, Oral presentation
  [Invited]
• Laser-assisted pseudo-biomineralization for tooth surface functionalization
  A Oyane; A Narazaki; H Miyaji
  2022 CLEO conference, 15 May 2022, English, Oral presentation
  [Invited]
• Tooth surface functionalization via laser-assisted pseudo-biomineralization
  A Oyane; I Sakamaki; S Santhakumar; H Miyaji
  SFB+JSB joint symposium 2022, Jan. 2022, English
  08 Jan. 2022 - 10 Jan. 2022
• Delivery of protein-immobilized calcium phosphate microchips to a human dentin by laser-Induced forward transfer with optical stamp
  A Narazaki; A Oyane; H Miyaji
  8th ABMC, Nov. 2021, English, Oral presentation
  28 Nov. 2021 - 30 Nov. 2021
• Secreted frizzled-related protein 1 promotes odontoblastic differentiation and reparative dentin formation in dental pulp cells
  K Ipposhi; A Tomokiyo; T Ono; K Yamashita; MA Alhasan; H Miyaji; D Hasegawa; S Hamano; S Yoshida; H Sugii; T Itoyama; M Ogawa; T Sugaya; H Maeda
  The 19th JEA-KAE Joint Scientific Meeting, Oct. 2021, English
  29 Oct. 2021 - 04 Nov. 2021
• Aggregation/self-assembly controlled gold nanocluster - biopolymer conjugates for therapeutic applications
  H Kawasaki; K Shitomi; I Okamoto; H Miyaji
  APT 2021, Oct. 2021, English
  11 Oct. 2021 - 14 Oct. 2021
• Gold nanocluster -decorated biopolymeric hybrid particles for antibacterial photodynamic therapy
  H Kawasaki; K Shitomi; I Okamoto; H Miyaji
  ISST 2021, Aug. 2021, English
  05 Aug. 2021 - 06 Aug. 2021
• Characterization of human root cementum coated with a fluoride-incorporated calcium phosphate layer by the laser-assisted biomimetic process
  S Tanaka; H Miyaji; T Tanaka; S Inoue; A Oyane
  99th IADR, Jul. 2021, English, Oral presentation
  21 Jul. 2021 - 24 Jul. 2021, 24539350
• Gold and silver nanoclusters for biomedical applications
  H Kawasaki; Y Iwasaki; H Miyaji
  BMI 2021, May 2021, English, Invited oral presentation
  30 May 2021 - 03 Jun. 2021, 24539354, [Invited]
• The biocompatibility and eugenol release of two root canal sealers
  K Ono; N Mitani; K Shitomi; H Miyaji; T Sugaya; T Kumagai
  98th IADR, Mar. 2020, English, Poster presentation
  18 Mar. 2020 - 21 Mar. 2020
• Laser-induced forward transfer of bioactive materials with PDMS-based optical stamp
  A Narazaki; A Oyane; H Miyaji
  Photonics WEST 2020, Feb. 2020, English, Oral presentation
  01 Feb. 2020 - 06 Feb. 2020, 24539353
• In situ fabrication of amorphous calcium phosphate nanoparticles within 3D collagen sponges for bone tissue engineering
  S Santhakumar; A Oyane; M Nakamura; K Koga; S Miyata; K Muratsubaki; H Miyaji
  PACRIM13, Oct. 2019, English, Poster presentation
  27 Oct. 2019 - 01 Nov. 2019
• Additive Patterning of Fibronectin-Immobilized Apatite Micro-Chips by Laser-Induced Forward Transfer
  A Narazaki; A Oyane; R Kurosaki; T Kameyama; I Sakamaki; H Araki; H Miyaji
  PACRIM13, Oct. 2019, English, Poster presentation
  27 Oct. 2019 - 01 Nov. 2019
• Laser-assisted pseudo-biomineralization on human dentin for tooth surface coating
  A Oyane; I Sakamaki; M Nakamura; K Koga; K Shitomi; K Mayumi; H Miyaji
  PACRIM13, Oct. 2019, English, Poster presentation
  27 Oct. 2019 - 01 Nov. 2019
• Laser additive patterning for biomedical applications by laser-induced transfer
  A Narazaki; A Oyane; H Miyaji
  ICALEO 2019, Oct. 2019, English, Oral presentation
• Bio-active patterning of fibronectin-containing apatite by laser-induced forward transfer
  A Narazaki; A Oyane; H Miyaji
  LAMP 2019, May 2019, English, Oral presentation
• Laser-induced forward transfer of bioactive apatite immobilizing proteins
  A Narazaki; R Kurosaki; T Kameyama; I Sakamaki; H Araki; A Oyane; H Miyaji
  Photonics WEST 2019, Feb. 2019, English
• Laser-lift-off process for additive micropatterning of functional particles and films
  A Narazaki; T Sato; H Niino; Y Nakata; T Shojo; Y Tsuboi; A Oyane; H Miyaji
  MHS 2018, Dec. 2018, English
  [Invited], [International presentation]
• Calcium phosphate coating on human dentin and composite resin surface by laser-assisted biomimetic process
  S Tanaka; H Miyaji; E Nishida; AJ Nathanael; M Nakamura; A Oyane; T Tanaka; S Inoue
  20th KACD-JSCD, Nov. 2018, English, Poster presentation
  [International presentation]
• Acid-resistance and antibacterial properties of laser-processed and fluoride-incorporated apatite layer
  K Shitomi; H Miyaji; AJ Nathanael; M Nakamura; A Oyane; T Sugaya
  104th AAP, Oct. 2018, English
  [International presentation]
• Fluoride-incorporated apatite coating for tooth surface modification by laser irradiation in supersaturated solutions
  A Oyane; AJ Nathanael; M Nakamura; K Shitomi; H Miyaji
  Bioceramics 30, Oct. 2018, English, Oral presentation
  [International presentation]
• Application of S-PRG nanofillers for novel periodontal therapeutic approach
  H Miyaji; K Mayumi; E Nishida; S Miyata; K Shitomi; T Furihata; Y Kanemoto; T Akasaka; T Sugaya
  104th AAP, Oct. 2018, English, Poster presentation
  [International presentation]
• Aggregation/self-assembly-induced approach for efficient AuAg bimetallic nanocluster-based photosensitizers
  D Hikoso; S Saita; S Miyata; H Miyaji; T Furuike; H Tamura; H Kawasaki
  ISST 2018, Aug. 2018, English
  [International presentation]
• Apatite coating based on laser-induced forward transfer for dental treatment
  A Narazaki; R Kurosaki; I Sakamaki; A Oyane; H Miyaji
  LPM 2018, Jun. 2018, English, Oral presentation
  [International presentation]
• Apatite-coated collagen sponges for tissue engineering applications
  AJ Nathanael; A Oyane; M Nakamura; H Miyaji; E Nishida; Y Kanemoto
  IUMRS-ICAM 2017, Aug. 2017, English, Oral presentation
  [International presentation]
• Biomedical assessment of titanium coated with carbon nanotubes
  H Miyaji; E Nishida; J Umeda; K Kondoh; H Takita; T Iizuka; T Akasaka; S Tanaka; A Kato; B Fugetsu; T Sugaya
  Visual-JW 2016, Oct. 2016, English
  [Invited]
• The effect of protein adsorption on cell alignment of human gingival fibroblast on the grooved composite resins
  Akasaka T; Miyaji H; Kaga N; Yokoyama A; Yoshida Y
  AsiaNANO 2016, Oct. 2016, English
  [International presentation]
• One-pot Process for Fabricating Calcium Phosphate Submicrospheres Loaded with Silver Nanoparticles Utilizing Laser Light
  M Nakamura; A Oyane; Y Shimizu; H Miyaji
  ABC 2015, Dec. 2015
• Bioactivity of Chitosan/ Gelatin Scaffold Cross-linked by GlcNAc System
  T Yoshida; H Miyaji; E Nishida; A Kato; T Chaochai; T Furuike; H Tamura; M Kawanami
  93rd IADR, Mar. 2015, English, Oral presentation
  [International presentation]
• Biocompatibility of CNT-Ti composite surface with different nanomorphologies
  E Nishida; H Miyaji; J Umeda; K Kondoh; H Takita; B Fugetsu; S Tanaka; A Kato; T Akasaka; M Kawanami
  93rd IADR, Mar. 2015, English, Oral presentation
  [International presentation]
• Bioactive titanium surface using carbon nanotube net film
  H Miyaji; E Nishida; J Umeda; K Kondoh; H Takita; B Fugetsu; S Tanaka; A Kato; T Akasaka; M Kawanami
  Visual-JW 2014, Nov. 2014, English, Nominated symposium
  [Invited], [International presentation]
• Preparation and properties of gelatin sponge for biomaterial application
  T Chaochai; H Miyaji; T Yoshida; E Nishida; T Furuike; H Tamura
  PETROMAT and PPC SYM 2014, Apr. 2014
• Graphene oxide for tissue engineering application
  E Nishida; H Miyaji; H Takita; I Kanayama; M Tsuji; M Kawanami
  MNC 2013, 05 Nov. 2013, English, Oral presentation
  [International presentation]
• Preparation of chitosan/ gelatin based biomaterial.
  T Chaochai; H Miyaji; T Yoshida; E Nishida; T Furuike; H Tamura
  10th International Conference of the Asian Pacific Chitin Chitosan Symposium, 04 Oct. 2013, English, Poster presentation
  [International presentation]
• Preparation and biological evaluation of titanium coated with CNT-net
  E Nishida; H Miyaji; J Umeda; K Kondoh; B Fugetsu; S Tanaka; A Kato; I Kanayama; M Kawanami
  98th AAP, Sep. 2012
• The bone-forming effect of nano-β-TCP/collagen scaffold
  A Kato; H Miyaji; Y Kosen; K Ogawa; T Momose; B Fugetsu; A Ibara; T Yoshida; S Tanaka; M Kawanami
  98th AAP, Sep. 2012
• Effect of β-TCP foam cell size on osteoinduction using FGF2
  H Miyaji; H Yokoyama; Y Kosen; A Ibara; H Nishimura; M Kondo; N Kobayashi; K Tokunaga; S Tanaka; M Kawanami
  88th IADR, Jul. 2010
• Periodontal regeneration following BMP-root conditioning and collagen scaffold implantation
  A Kato; H Miyaji; R Ishizuka; K Tokunaga; K Inoue; Y Kosen; H Yokoyama; S Tanaka; T Sugaya; M Kawanami
  88th IADR, Jul. 2010
• Bone marrow cell sheet in BMP-applied collagen scaffold for bone tissue engineering
  K Inoue; H Miyaji; H Fukuda; H Yokoyama; S Tanaka; T Sugaya; M Kawanami
  11th KACD-JSCD, Nov. 2009
• Osteoinduction following implantation of collagen hydrogel-sponge composite
  H Miyaji; T Sugaya; K Tokunaga; N Kobayashi; S Shimoji; K Inoue; Y Kosen; M Kawanami
  86th IADR, Jul. 2008, English, Poster presentation
  [International presentation]
• Properties of 4-META/MMA-TBB resin mixed with dentin powder
  S Tanaka; T Sugaya; H Miyaji; Y Ibe; T Tenkumo; H Fukuda; T Washizu; Y Yoshimura; M Kawanami
  86th IADR, Jul. 2008, English, Poster presentation
  [International presentation]
• Healing after root-end sealing using 4-META/MMA-TBB resin following apicoectomy
  K Ohtani; T Sugaya; M Tomita; Y Hasegawa; Y Motoki; H Miyaji; Y Takanawa; M Kawanami
  86th IADR, Jul. 2008, English, Poster presentation
  [International presentation]
• Effect of decortication and collagen sponge implants on bony augmentation
  S Shimoji; H Miyaji; T Sugaya; M Kawanami; H Tsuji; T Hongo
  92nd AAP, Sep. 2006, English, Poster presentation
  [International presentation]
• Periodontal tissue reaction on BMP application to root dentin surface
  H Miyaji; T Sugaya; K Ibe; R Ishizuka; K Tokunaga; M Kawanami
  84th IADR, Jun. 2006, English, Poster presentation
  [International presentation]
• Changes in biocompatibility of 4-META/MMA-TBB resin in vitvo
  S Tanaka; T Sugaya; K Ohtani; H Miyaji; K Tokunaga; M Kawanami
  84th IADR, Jun. 2006, English, Poster presentation
  [International presentation]
• Prognosis of periradicular surgery using 4-META/MMA-TBB resin as root-end sealant
  T Sugaya; H Noguchi; H Miyaji; M Kawanami
  81st IADR, Jun. 2003, English
  [International presentation]
• Dentin resorption and hard tissue formation following implantation of BMP-2 applied dentin
  H Miyaji; T Sugaya; K Kato; N Kawamura; H Tsuji; M Kawanami
  81st IADR, Jun. 2003, English
  [International presentation]
• ALP and proliferation of gingival fibroblast on BMP-2 applied dentin
  T Sugaya; H Miyaji; H Kato
  76th IADR, Jun. 2001, English
  [International presentation]

• 歯周病学, 2024年, 博士後期課程, 歯学院
• 歯周組織再建外科学, 2024年, 博士後期課程, 歯学院
• 口腔健康科学実習Ⅳ, 2024年, 博士後期課程, 歯学院
• 歯周組織再建学研究Ⅰ, 2024年, 博士後期課程, 歯学院
• 歯周組織再建学研究Ⅱ, 2024年, 博士後期課程, 歯学院
• 口腔健康科学実習Ⅰ, 2024年, 博士後期課程, 歯学院
• 口腔健康科学実習Ⅱ, 2024年, 博士後期課程, 歯学院
• 口腔健康科学実習Ⅲ, 2024年, 博士後期課程, 歯学院
• 口腔健康科学実習Ⅴ, 2024年, 博士後期課程, 歯学院
• 歯周病学研究Ⅰ, 2024年, 博士後期課程, 歯学院
• 歯周病学研究Ⅱ, 2024年, 博士後期課程, 歯学院
• 口腔生物学と医学, 2024年, 博士後期課程, 歯学院
• 歯学研究概論, 2024年, 博士後期課程, 歯学院
• 歯周病学・歯内療法学臨床実習Ⅰ, 2024年, 学士課程, 歯学部
• 歯周病学・歯内療法学臨床実習Ⅱ, 2024年, 学士課程, 歯学部
• 歯周病学・歯内療法学基礎実習, 2024年, 学士課程, 歯学部
• 歯周病学・歯内療法学, 2024年, 学士課程, 歯学部
• 総合臨床基礎実習, 2024年, 学士課程, 歯学部
• 臨床講義Ⅰ, 2024年, 学士課程, 歯学部

• Sep. 2023 - Present
  Japanese Society of the General Dentistry
• Dec. 2022 - Present
  Japanese Dental Education Association
• Feb. 2021 - Present
  Hokkaido Osteoporosis Society
• Apr. 2014 - Present
  THE JAPANESE SOCIETY FOR DENTAL MATERIALS AND DEVICES
• 2010 - Present
  THE JAPANESE SOCIETY FOR BIOMATERIALS
• 2010 - Present
  代用臓器・再生医学研究会
• Apr. 2000 - Present
  The Japanese Society of Conservative Dentistry
• Jun. 1997 - Present
  The Japanese Society of Periodontology
• 1997 - Present
  北海道歯学会
• 2002 - Mar. 2027
  THE JAPANESE SOCIETY FOR REGENERTIVE MEDICINE (JSRM)
• Aug. 2022 - Mar. 2025
  日本レーザー歯学会
• Apr. 2022 - Mar. 2025
  JAPAN ENDODONTIC ASSOCIATION
• Jun. 2015 - Mar. 2025
  THE JAPANESE ACADEMY OF CLINICAL PERIODONTOLOGY
• 2010 - Mar. 2022
  日本再生歯科医学会
• 2006 - 2022
  IADR
• 2009 - 2019
  NANO BIOMEDICAL SOCIETY

• LABプロセスを用いたインプラント周囲炎の革新的再生治療法創出
  科学研究費助成事業
  Apr. 2025 - Mar. 2028
  西田 絵利香; 宮治 裕史; 蔀 佳奈子; 黒嶋 伸一郎; 大矢根 綾子; 島袋 将弥
  日本学術振興会, 基盤研究(C), 北海道大学, 25K13061
• Laser-processed antimicrobial surfaces contribute to health and longevity! The New Development in the Treatment of Oral Candidiasis
  Grants-in-Aid for Scientific Research
  Apr. 2025 - Mar. 2028
  田中 佐織; 宮治裕史; 大矢根綾子; 西田絵利香
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 25K12999
• レーザーで軟化象牙質をアパタイトに置換する－根面う蝕治療法の新規開発
  科学研究費助成事業
  Apr. 2025 - Mar. 2028
  宮治裕史; 大矢根綾子; 松田康裕; 西田絵利香; 蔀佳奈子
  日本学術振興会, 基盤研究(B), 北海道大学, 25K02808
• インプラント周囲炎治療のための抗菌・骨形成促進ナノ粒子の術中3次元デリバリー
  科学研究費助成事業
  Apr. 2024 - Mar. 2028
  中村 真紀; 奈良崎 愛子; 宮治 裕史; 西田 絵利香
  日本学術振興会, 基盤研究(B), 国立研究開発法人産業技術総合研究所, 24K03292
• 根面う蝕制圧のためのナノ粒子レーザー転写による持続的な歯面抗菌化技術の創成
  公益財団法人 村田学術振興・教育財団研究助成
  Jul. 2024 - Jun. 2027
  中村真紀,宮治裕史
  Coinvestigator
• 天然歯・人工歯の表面へのパターン付与治療へ向けた歯周組織再生の形態ロジック構築
  科学研究費助成事業
  01 Apr. 2024 - 31 Mar. 2027
  赤坂 司; 横山 敦郎; 吉村 善隆; 宮治 裕史; 牛島 夏未; 星 淡子
  日本学術振興会, 基盤研究(B), 北海道大学, 23K28422
• 骨再生を目的としたエリスロマイシン含有マイクロ粒子製剤開発
  Apr. 2025 - Mar. 2027
  前川知樹; 宮治裕史
  橋渡し研究プログラムPreF, Coinvestigator
• 人工バイオミネラリゼーションの光制御と歯面機能デザイン
  科学研究費助成事業
  Apr. 2024 - Mar. 2027
  大矢根 綾子; 宮治 裕史; 西田 絵利香
  日本学術振興会, 基盤研究(B), 国立研究開発法人産業技術総合研究所, 24K03294
• Photo-regenerative therapy - biomimetic cementum construction on periodontal root surfaces by laser irradiation
  Grants-in-Aid for Scientific Research
  01 Apr. 2023 - 31 Mar. 2026
  加藤 昭人; 大矢根 綾子; 宮治 裕史; 田中 佐織; 金本 佑生実
  今年度は，歯基材へのコラーゲン層構築の検討およびコラーゲン層へのLABプロセスによる人工セメント質の構築を行った．
  具体的にはゲニピンーコラーゲン象牙基材を作成し，改良塗布法でコラーゲン層にアパタイトが生成するかどうかの実験を行った．
  ①Makitaらの方法を参考に，ゲニピン-コラーゲンを作製 ②2mLの20wt％コラーゲン（ゼラチン from porcine skin，Sigma-Aldrich，G1890-100G)にゲニピン（富士フィルム和光純薬，078-03021）の最終濃度が20mMになるように添加し，60度に加熱，撹拌した． ③ゲル状になるまで室温で冷ました後，象牙質チップ（厚み1mm）に塗布した． ④ -80度で凍結させた後，凍結乾燥機で１日フリーズドライした． ⑤インドシアニングリーン（ジアグノグリーン）にピペットを用いて7wt%グリセリン溶液0.2mLを加え溶解させた． ⑥マイクロアプリケーターにて調整したインドシアニングリーンをチップに塗布し，0.5mm調整専用ジグで表面をならした． ⑦F1000CP液を調整し，Sレーザーを次の条件で照射した．ファイバーと試料の距離：3mm 溶液：F1000CP液10mL 照射パワー：3W 照射時間：30秒 ⑧Slaser照射試料のSEM，EDS測定を実施した．
  結果と考察 照射後の象牙質チップの表面に残存ICG由来のS及びNaが検出され，Fは非検出であった．原因としては試料表面の曲率が大きく，ICG膜厚のコントロールは困難であったこと，もしくはコラーゲンに起因する可能性が考えられた．SEMにて表面に凹凸構造が観察された．今後はコラーゲン以外の天然高分子の利用を試みることと，コラーゲンの塗布方法の改良や照射条件の調整を行う予定である．
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 23K11821
• 看護・移動・歯科連携によるPHR活用型予防支援モデルの研究開発
  Apr. 2025 - Mar. 2026
  中田亜由美; 宮治裕史; 岩崎正則; 渡邊 裕; 貝谷敏子; 松舘 渉; 野田五十樹; 土作正敏; 吉田 学
  ノーステック財団先端医療産業促進補助金（デジタルヘルス分野）, Coinvestigator
• キトサンナノゲル抗菌剤の開発実証
  橋渡し研究プログラムシーズA
  Apr. 2023 - Mar. 2025
  長谷川智香; 宮治裕史; 長谷部 晃; 網塚憲生; 西田絵利香; 蔀佳奈子; 林宏至
  Coinvestigator
• 二段階光反応を利用したレジン修復歯面の強化エナメル改質と二次う蝕制圧
  科学研究費助成事業
  Jun. 2022 - Mar. 2025
  大矢根 綾子; 宮治 裕史
  う蝕（虫歯）治療の最大の課題は、レジン等の修復物との隙間に侵入したう蝕原因菌による二次う蝕（再発）である。本研究の目的は、研究代表者らが開発してきた2種類の光技術（（１）レジンの光重合を利用した膜転写法、（２）過飽和液中レーザー照射法）を利用し、レジン修復歯面をフッ素含有アパタイトで被覆・高機能化するための技術を開発することである。
  当該年度（2022年度）は、上記（１）および（２）の技術開発をそれぞれに進めた。（１）では、研究代表者らの既報（Hibino et al., Mater Sci Eng C, 2020）をベースとし、リン酸カルシウム過飽和溶液（原料膜の合成液）にフッ化ナトリウムを添加することで、レジン圧接用（膜転写用）の透明高分子フィルム上にフッ素含有アパタイト原料膜を合成した。（２）では、研究代表者らの既報（Oyane et al., Inter J Mol Sci, 2022）をベースとし、硬化レジンへの前処理として表面にインドシアニングリーン（光吸収剤となる色素薬剤）を塗布した後、フッ化ナトリウムを添加したリン酸カルシウム過飽和溶液中で歯科用半導体レーザー（波長：808 nm）を照射した。その結果、一定の前処理・照射条件下において、照射面の一部に、フッ素含有アパタイトを成膜できることが分かった。レジン修復面の全面成膜・高機能化に向けて、足掛かりとなる予備的な結果を得た。
  日本学術振興会, 挑戦的研究(萌芽), 国立研究開発法人産業技術総合研究所, Coinvestigator, 22K19927
• Aiming to create an antimicrobial coated root surface! ~Development of a New Periodontal Disease Treatment Method Applying Biomimetic Methods~.
  Grants-in-Aid for Scientific Research
  Apr. 2022 - Mar. 2025
  田中 佐織; 阿部 貴惠; 大矢根 綾子; 宮治 裕史
  本研究では，新しい歯周病予防法及び進行抑制法を開発，実現することを目的として，レーザー援用バイオミネラリゼーション法（Laser-assisted biomineralization process: LABプロセス）を応用し，歯周ポケット内の汚染セメント質に「抗菌加工」を行うことに着想した．LABプロセスは，擬似バイオミネラリゼーション（過飽和溶液中でのアパタイト析出反応）をレーザー光照射で促進する応募者らの独自技術であり，歯面に形成されるアパタイトに抗菌性を付与することが可能である．
  本研究は，過飽和溶液にフッ素，亜鉛，銀を添加して，アパタイトとの複合化することで，世界初の歯周病抗菌治療法を実現することを目指している．
  令和4年度はヒトセメント質を用いてLAB法（リン酸カルシウム過飽和溶液中に浸漬，医療機器認可済の半導体レーザー（808 nm, Power: 3 W, ファイバー径 300μm）照射）により最適なアパタイトの成膜を試みた．適切な条件設定を行い、物性評価を行い，さらに抗菌性を有するAgを複合化し，物性評価を行った．抗菌性物質の複合化の方法は，試料としてヒトセメント質を用いて，サホライド（フッ化ジアミン銀）とレーザー光吸収色素を塗布後，フッ素を含まない過飽和Cap溶液中でレーザー照射を行ったが，Cap膜の生成は確認されなかった．
  臨床応用を目指して根面に適切な照射条件を検討するために歯列模型を使用しポケット内へレーザー照射を行い，照射距離・角度と照射範囲を計測，照射条件を検討した．歯肉の形態によりポケット内照射角度が根面に平行となり，根面に照射するのがやや困難であった．
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Coinvestigator, 22K09976
• ２段階薬剤放出性第４世代スキャフォールドの歯周組織再生への応用
  科学研究費助成事業
  Apr. 2022 - Mar. 2025
  西田 絵利香; 宮治 裕史; 大矢根 綾子; 中村 真紀; 赤坂 司
  本研究では，段階的に薬剤を放出する新世代（第４世代）スキャフォールドを創出し，歯周組織再生療法への導入を目的としている．つまり，溶解性の異なる２層構造アパタイトをコラーゲンスポンジと複合化して，優れた再生能を示す最先端の第４世代スキャフォールドの創出を目指している．
  昨年度までに，１層目として，アパタイトの難溶化（溶解性低下）と抗菌性の期待される生体微量元素（フッ素）を種々の濃度（０～１０００μＭ）でＣａＰ過飽和溶液に添加し，スキャフォールド上にフッ素含有アパタイト層を作製した．フッ化物イオンをバイオミメティックコーティング法によりアパタイト層に取り込むことに成功し，フッ化物濃度の増加とともにアパタイト層の溶解度が低下し，減少した．しかし，フッ素濃度が高くなると生体為害性が出現したため，フッ素の添加は不適合という判断になった．
  そこで新しく，炭酸アパタイト（ＣａＰ）を析出させたスキャフォールドにさらにナノＣａＰ粒子をつけることで，ナノ粒子が超早期に，ＣａＰが早期に溶出し，２段階となるようにできないか検討を行った．
  まずはＣａＰナノ粒子にＦＧＦを添加し，ＦＧＦの至適濃度を評価した．ＦＧＦ濃度０，５，５０μｇ/ｍＬで水懸濁液を作製した．ラット頭蓋骨にφ５ｍｍの欠損を作製し，ＣａＰ顆粒に作製した懸濁液を混合して，欠損部に補填し，縫合した．４週後に屠殺してＣＴ撮影と組織学的評価を行った．ＦＧＦ０μg/mLでは骨新生はほとんど認められず，ＦＧＦ５μｇ/ｍＬおよび５０μｇ/ｍＬで骨新生を認めた．特にＦＧＦ５μｇ/ｍＬで最も骨新生を認めた．ＦＧＦの至適濃度が概ねわかってきたので，今後はナノ粒子へのＦＧＦ複合化を内包型ではなく，担持型にすべく条件を検討中である．
  日本学術振興会, 基盤研究(C), 北海道大学, Coinvestigator, 22K10012
• CMキトサンCPC複合ナノゲル剤を用いた口腔ケア製品の開発実証
  ノーステック財団札幌バイオシーズ事業化支援補助金
  Apr. 2023 - Mar. 2024
  宮治裕史; 小野寺真也; 中島愛梨; 渡邉 弓; 長谷川智香; 長谷部 晃; 林 宏至; 網塚憲生
  Principal investigator
• 歯周病光殺菌治療のための光感応性キトサンナノゲル剤の開発
  ノーステック財団「札幌ライフサイエンス産業活性化事業」事業化支援補助金
  Apr. 2022 - Mar. 2023
  加藤昭人; 宮治裕史; 長谷部晃; 網塚憲生; 小野寺真也; 中島愛梨; 渡邉弓
  Coinvestigator
• 非熱的レーザー射出を利用した抗菌性バイオミネラルナノ粒子による歯根面の三次元修復
  公益財団法人天田財団一般研究開発助成
  Apr. 2022 - Mar. 2023
  中村真紀; 奈良崎愛子; 宮治裕史
• 動物の歯を利用した脱灰象牙質由来コラーゲン顆粒骨再生材料の研究開発
  橋渡し研究プログラムシーズB
  Apr. 2021 - Mar. 2023
  大久保直登; 横山敦郎; 宮治裕史
  Coinvestigator
• Tooth surface functionalization via optical control of interfacial reactions for advanced periodontal treatments
  Grants-in-Aid for Scientific Research
  Apr. 2020 - Mar. 2023
  OYANE Ayako
  A technique for fluoridated apatite (FAp) coating on teeth would be useful in dental applications. In this study, we achieved rapid (in 30 min) FAp coating on a human dentin substrate by a laser-assisted biomimetic (LAB) process; pulsed Nd:YAG laser irradiation in a supersaturated calcium phosphate solution supplemented with NaF. The LAB-processed, FAp-coated dentin substrate exhibited antibacterial activity against Streptococcus mutans. We refined the LAB process with a combination of a dental diode laser and a clinically approved light-absorbing agent, indocyanine green, and achieved even faster (in 3 min) FAp coating on the human dentin substrate. Cross-sectional analysis by transmission electron microscopy revealed gapless interface states between the FAp coating layer and the dentin substrate. The LAB process enables rapid FAp coating on a target region of the dentin surface, and hence, has potential as a tool for tooth surface functionalization.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), National Institute of Advanced Industrial Science and Technology, Coinvestigator, 20H04541
• Strengthened enamel formation via laser irradiation in a supersaturated solution for the prevention of tooth caries
  Grants-in-Aid for Scientific Research
  Jun. 2019 - Mar. 2023
  OYANE Ayako
  The human tooth enamel is comprised of well-organized apatite nanocrystals. Dissolution of enamel apatite (demineralization) can be a cause of dental decay. To prevent irreparable demineralization, fluoride ions are applied to a tooth surface to increase acid-resistance of enamel apatite through partial ionic substitution. In this study, we demonstrated rapid formation of a fluoride-incorporated apatite layer on a human enamel substrate via laser-assisted pseudo-biomineralization. In this process, pulsed laser or dental diode laser (in combination with light absorbing agent) was used to irradiate the enamel surface to accelerate pseudo-biomineralization in a supersaturated calcium phosphate solution supplemented with NaF. The thus-formed surface layers consisted of enamel-like needle-like apatite nanocrystals with c-axis orientation and contained fluoride ions throughout the layer. The present process would be a useful new tool to protect and strengthen the enamel surface.
  Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Research (Exploratory), National Institute of Advanced Industrial Science and Technology, Coinvestigator, 19K22991
• Development of peri-implantitis treatment technology that enables regeneration of the periodontal ligament only by applying ultrastructural micro/nano-patterns
  Grants-in-Aid for Scientific Research
  Apr. 2019 - Mar. 2023
  Akasaka Tsukasa
  The effects of pillar, groove, and hole patterns on osteoclasts, osteoblasts, periodontal ligament fibroblasts, and blood cells were investigated as the effect on cell behaviors. As a result, the patterns with high and low effects were found. Several types of regulations of the resorption, calcification, and the orientation of cells and collagen fibers by patterns have been achieved. Animal studies have also shown that the activities of the patterns vary depending on the implantation places and that the tissue response is dependent on the pattern size and shape.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Coinvestigator, 19H04461
• Biomimetic apatite coating on root surface for prevention of root caries
  Grants-in-Aid for Scientific Research
  Apr. 2019 - Mar. 2023
  TANAKA SAORI
  The aim of this study was to develop a new method of root surface caries prevention and control by coating antimicrobial apatite on exposed root cementum using a laser-assisted biomimetic technique.
  1.The surface analysis of the laser irradiated cementitious substrate in supersaturated CaP solution with NaF showed that F was detected on the irradiated surface, and the formation of fluorine-containing apatite was confirmed from the increase in the Ca/C element ratio. 2. Cementitious materials in supersaturated solution of CaP with NaF were irradiated with a semiconductor laser. FAp was deposited on the irradiated surface, and a significant increase in Knoop hardness was observed compared to the unirradiated surface. 3. Demineralization inhibiting effect was confirmed. 4. The possibility of intraoral application was found from experiments using beagle dogs.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 19K10103
• Development of quantum dot-sonosensitizer and its application to treatment of intractable infections
  Grants-in-Aid for Scientific Research
  Apr. 2019 - Mar. 2022
  Kawasaki Hideya
  In this study, we aimed to develop a treatment method to kill cells and bacteria locally by generating singlet oxygen (1O2) under ultrasonication, one of the reactive oxygen species (sound sensitization), through the excitation of gold nanoclusters (tens of gold atom aggregates) by high energy cavitation bubbles generated by ultrasound irradiation. The ligand and size effects of Au NCs were clarified as a guideline for the particle design of Au NCs with ultrasound sensitizing activity. We demonstrated that not only high 1O2 production by Au NCs themselves but also small 1O2 loss due to autoxidation by 1O2 is important to achieve high 1O2 production efficiency by ultrasonically excited Au NCs. As for the size-effect(Au25, Au38, Au144, and Au300~500), Au144 NCs, which have properties intermediate between those of metals and molecules, exhibit the highest sound sensitizing activity.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Kansai University, Coinvestigator, 19H02564
• Development of material delivery method by laser-induced nanojet and creation of high-performance artificial tooth surface
  Grants-in-Aid for Scientific Research
  Apr. 2019 - Mar. 2022
  Narazaki Aiko
  Periodontal disease is a lifestyle-related disease with an extremely high prevalence, and if the treatment method becomes more sophisticated, it will contribute to improving the QOL. In the regenerative treatment of periodontal disease, it is important to reconstruct the attachment (periodontal attachment) to the periodontal tissue on the tooth surface destroyed by bacterial growth at an early stage. In this work, we have developed a bio-ink in which nano-composite particles of physiologically active substances and calcium phosphate are dispersed in high concentration, and a novel laser-induced jet is used to form a highly functional artificial tooth surface using the bio-ink. Aiming to build a three-dimensional delivery technology for nano-composite particles, we succeeded in depositing bio-ink on a substrate with a disk shape of about 50 micrometers with high positional accuracy.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), National Institute of Advanced Industrial Science and Technology, Coinvestigator, 19H02635
• Development of the new gel agent for periodontal disease treatment combining photo-sterilization and tissue regeneration effects
  Grants-in-Aid for Scientific Research
  Apr. 2019 - Mar. 2022
  Kato Akihito
  Antimicrobial photodynamic therapy (a-PDT) is a treatment method that uses light irradiation and singlet oxygen generated by a photosensitizer to kill bacteria. In this study,gold and silver nanoclusters of photosensitizers and rose bengal nanogels containing chitosan, a cationic polymer, were prepared and their photodynamic properties were investigated. The photodynamic properties of the nanogels were investigated, and their antibacterial and antibiofilm properties and cytotoxicity were also evaluated.The results suggest that a-PDT using white LED irradiation may be effective against oral biofilm infections.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Coinvestigator, 19K10102
• キトサンナノゲルと近赤外光を利用した光殺菌性歯周病治療法の開発
  2019年度研究助成（一般）
  Apr. 2019 - Mar. 2020
  宮治裕史,川﨑英也
  公益財団法人秋山記念生命科学振興財団, Principal investigator, Competitive research funding
• 新規な歯周病再生用骨補填材の開発
  研究戦略的推進プログラムシーズA
  Apr. 2019 - Mar. 2020
  宮治 裕史
  Principal investigator, Competitive research funding
• 歯周ポケット内の持続的な除菌治療のための多機能性ナノ粒子の創製
  調査研究助成金
  Apr. 2019 - Mar. 2020
  中村真紀; 大矢根綾子; 宮治裕史
  公益財団法人鈴木謙三記念医科学応用研究財団, Coinvestigator, Competitive research funding
• Tooth surface functionalization via laser-assisted facile coating of apatite nanocomposite layers for periodontal treatments
  Grants-in-Aid for Scientific Research
  Apr. 2017 - Mar. 2020
  Oyane Ayako
  We developed two laser processes (laser-assisted biomimetic process and laser-induced film transfer process) to produce nanocomposite coatings consisting of crystalline apatite (main component of human teeth) and biofunctional substances. Utilizing the two laser processes, we achieved rapid (< 30 min), facile, and area-specific nanocomposite coating on substrates of several artificial materials. The nanocomposite-coated substrates exhibited improved biological function (cytocompatibility, antibacterial activity, acid resistance, etc.). Moreover, we demonstrated apatite coating on substrates of human dentin (prepared from extracted teeth after obtaining informed consent) by the two laser processes.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), National Institute of Advanced Industrial Science and Technology, Coinvestigator, 17H02093
• Fabrication and application of nanomaterial using biomineralization method for periodontal tissue engineering
  Grants-in-Aid for Scientific Research
  Apr. 2016 - Mar. 2019
  Miyaji Hirofumi; OYANE Ayako
  We fabricated the nano-structured apatite scaffold using biomineralization method to imitate the bone formation in the body. The evaluation of biocompatibility, bone inductive effect and periodontal regenerative activity of apatite scaffold were carried out. In addition, we obtained the apatite scaffold loaded with fibroblast growth factor-2 (FGF2) to enhance the bio-properties of scaffold and assessed the bone forming ability.
  The results showed that apatite scaffold exhibited high biocompatibility and bone forming effect in rat cranial bone defect. Apatite scaffold stimulated the periodontal regeneration in class II furcation defect in beagle dogs. In addition, FGF-2 loaded apatite scaffold remarkably promoted the bone augmentation of rat skull bone, compared to pristine apatite scaffold. The nano-structured apatite scaffold would be beneficial for medical application in bone and periodontal tissue engineering.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 16K11822
• Create periodontal tissue formation on composite resin! for improvement of reliability of bonding treatment
  Grants-in-Aid for Scientific Research
  Apr. 2016 - Mar. 2019
  TANAKA SAORI; OYANE AYAKO; NAKAMURA MAKI; Nathanael AJ; KATO AKIHITO; MIYATA SAORI; MAYUMI KAYOKO
  Composite resin (CR) is applied as a bonding treatment and as a root perforation. However, CR surface rarely reconstructs periodontal attachment apparatus.
  The laser-assisted biomimetic (LAB) process.This may improve the biocompatibility of CR through the surface modification. This study aimed to develop a technique to produce the osteoconductive resin surfaces through calcium phosphate (CaP) coating using LAB process.
  The LAB-processed substrate was immersed for 3 days in a simulated body fluid (SBF) for the preliminary osteoconductivity assessment.According to the results of the microscopic observation and the EDX analysis, nano- and micro-structured CaP layers were formed on the CR surfaces, after the LAB process. Thus,this study might suggest a possibility of the periodontal tissue formation on CR.The LAB process is a potential new tool to create a cementum-like osteoconductive surface on dental composite resins.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Coinvestigator, 16K11538
• Novel Gold nanocluster-based Photosensitizers for Antimicrobial Photodynamic Therapy
  Grants-in-Aid for Scientific Research
  Apr. 2015 - Mar. 2018
  Kawasaki Hideya; TAMURA Hiroshi; FURUIKE Tetsuya
  In this study, we have devoted to the exploration of potential diagnostic applications of water-soluble gold nanoclusters (Au NCs) with ultra-small sizes (< 2 nm). One of the therapeutic applications is the photosensitized generation of reactive oxygen species using Au NCs as a photosensitizer for photodynamic therapy (PDT). We have reported Au NC photosensitizers and the effects of their size and type of surface ligands on singlet oxygen (1O2) generation. The photo-excited Au NC undergoes intersystem crossing (ISC) from its lowest singlet excited state (S1) to the lowest triplet excited state (T1). Subsequently, the transfer of energy from the T1 state of the Au NC to the ground-state oxygen (3O2) generates 1O2, which causes oxidative damage of targets. the photosensitizers based on the Au based photosensitizers were successfully used for antimicrobial-PDT (a-PDT) against bacteria.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Kansai University, Coinvestigator, 15H03520
• もみ殻由来ナノバイオシリカを用いた歯周病治療法の開発
  研究開発助成事業（札幌ライフサイエンス産業活性化事業・研究シーズ発掘補助金）
  Apr. 2016 - Apr. 2017
  西田絵利香; 宮治裕史; 近藤勝義; 梅田純子
  ノーステック財団, Coinvestigator, Competitive research funding
• Development of the therapeutic method for peri-implantitis using thin membranes with micro-nano structures
  Grants-in-Aid for Scientific Research
  Apr. 2014 - Mar. 2017
  Atsuro Yokoyama
  In this study, a nano imprint method was used to develop gelatin and titanium sheets with grooved and pillared structures. We investigated the effects of surfaces with micro and nano structures on adhesion and proliferation of osteoblasts, fibroblasts, and epithelial cells. However micro and nano structures accelerated adhesion and proliferation of osteoblasts and fibroblasts, those prevented epithelial cells. These results suggested that the micro and nano grooved and pillared structures effect on adhesion and orientation of osteoblasts, fibroblasts and epithelial cells.
  Japan Society for the Promotion of Science, Grant-in-Aid for Challenging Exploratory Research, Hokkaido University, 26670829
• 歯周病による重度組織破壊を再生する新規骨ペースト材の開発
  若手研究人材・ネットワーク育成補助金（Talent補助金）
  Apr. 2015 - Mar. 2016
  加藤昭人; 宮治裕史; 田中佐織; 岩崎泰彦
  ノーステック財団, Coinvestigator, Competitive research funding
• 歯周病治療用材料の開発
  橋渡し研究加速ネットワークプログラムシーズA
  Apr. 2015 - Mar. 2016
  宮治 裕史
  Principal investigator, Competitive research funding
• A development of the bonding treatment of vertically fractured roots with periodontal regeneration on adhesive resin using nanotechnology
  Grants-in-Aid for Scientific Research
  Apr. 2013 - Mar. 2016
  TANAKA SAORI; MIYAJI HIROFUMI; INOUE KANA
  Composite resin (CR) is used the bonding treatment of vertically fractured roots and as a retrograde material following apiccectomy. We aimed to the periodontal regeneration on CR. Effective biological modification of the CR surface is thus needed to improve its biocompatibility. Carbon nanotubes (CNT) and β-TCP have useful properties such as promoting cell adhesion and proliferation. CR discs were coated by immersion in a dispersion of CNT and β-TCP. In the cell culture assay, the CNT-CR samples exhibited abundant cells and marked adhesion of filopodia to CNT. Cell proliferation assay indicated that the CNT-CR was significantly higher than the CR . This results showed that coating the surface of CR with CNT improved their biocompatibility. In vivo studies, we observed the bone-like hard tissue formation on a CNT-CR wrapped in cultured bone marrow stromal cell sheets. Thus, this experiment might suggest a possibility of the periodontal regeneration on CR.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Coinvestigator, 25462943
• Novel periodontal therapy using nanocarbon coating procedure
  Grants-in-Aid for Scientific Research
  Apr. 2013 - Mar. 2016
  Miyaji Hirofumi; TANAKA SAORI; KATO AKIHITO; FUGETSU BUNSHI; NISHIDA ERIKA
  Biocompatibility tests were performed to evaluate the biological properties of nanocarbon materials, such as graphene oxide (GO) and carbon nanotube (CNT). In addition, tooth coating procedure using GO and regenerative scaffold including GO were developed for application to periodontal therapy. Low dose of GO and CNT consistently exhibited good biocompatibility, in particular, CNT stimulated cell proliferative activity. The tooth coating procedure using GO solution provided the antimicrobial effect to the tooth surface. GO scaffold exhibited cytocompatibility and promoted tissue healing and bone formation when implanted in tooth extraction socket or furcation periodontal defect in dog. Consequently, nanocarbon materials would be available for medical application in periodontal therapy.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Principal investigator, 25463210
• 新規歯面コーティング法の開発
  橋渡し研究加速ネットワークプログラムシーズA
  Apr. 2014 - Mar. 2015
  宮治 裕史
  Principal investigator, Competitive research funding
• in situでの骨治療に供するバイオアクティブ連通多孔質体の開発
  研究成果最適展開支援プログラムA-STEP
  Aug. 2013 - Mar. 2014
  岩﨑泰彦; 高畑祐輔; 宮治裕史
  JST, Coinvestigator, Competitive research funding
• カーボンナノチューブネットフィルムで表面改変したチタンの生体応用
  研究助成奨励
  Apr. 2012 - Mar. 2013
  宮治 裕史
  財団法人秋山記念生命科学振興財団, Principal investigator, Competitive research funding
• A development of the bonding treatment of vertically fractured roots with periodontal regeneration adhesive resin using tissue engineering
  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
  Apr. 2010 - Mar. 2013
  田中 佐織; 菅谷 勉; 宮治 裕史
  To enhance the bonding treatment of vertically fractured roots, we aimed to the newly cementum formation on a compound of resin and dentin powder, or β-TCP which may improve the binding between resin and cementum. In vitro, cytotoxicity was assessed. It had little cytotoxicity. In vivo, we observed the newly hard tissue formation on a resin containing β-TCP applied by rhBMP-2 and cultured bone marrow stromal cell sheets. This experiment might exhibit a possibility of the newly hard tissue formation on a resin.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Coinvestigator, Competitive research funding, 22592108
• Periodontal tissue engineering using osteoconductive scaffold coated by nano bioceramics.
  Grants-in-Aid for Scientific Research
  2010 - 2012
  MIYAJI Hirofumi; FUGETSU Bunshi; TANAKA Saori; KATO Akihito
  Nanoparticle bioceramics have many bioactive effects. We prepared collagen scaffold coated by nano tricalcium phosphate (TCP) and growth factor and evaluated the effects on tissue engineering. Nano-TCP scaffold showed higher compressive strength, cyto-compatibility and tissue ingrowth. In histological evaluations, bone augmentation and periodontal tissue healing were stimulated by application of nano-TCP scaffold in combination with fibroblast growth factor-2 (FGF2). We concluded that FGF2/nano-TCP scaffold would be effective for bone and periodontal tissue engineering.
  Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, 22791916
• 歯周組織再生を伴う垂直破折歯根の接着治療法の開発
  研究助成一般
  Apr. 2009 - Mar. 2010
  田中佐織; 菅谷 勉; 宮治裕史
  財団法人秋山記念生命科学振興財団, Coinvestigator, Competitive research funding
• Periodontal regeneration following BMP-root conditioning and collagen scaffold implantation.
  Grants-in-Aid for Scientific Research
  Apr. 2007 - Mar. 2010
  MIYAJI Hirofumi
  We examined whether combined therapy of BMP-conditioning and implantation of a collagen scaffold stimulated periodontal regeneration. The collagen scaffold was composed of collagen hydrogel and type I collagen sponge. Application of collagen scaffold promoted bone augmentation and periodontal regeneration with high biocompatibility and tissue replacing ability. Root surface conditioning with BMP and implantation of collagen scaffold sufficiently enhanced periodontal regeneration and rarely induced ankylosis. The combined therapy is expected to be a suitable therapy for periodontal tissue engineering.
  Japan Society for the Promotion of Science, Grant-in-Aid for Young Scientists (B), Hokkaido University, Principal investigator, 19791606
• A development of the bonding treatment of vertically fractured roots with cementum regeneration on 4-META/MMA-TBB resin
  Grants-in-Aid for Scientific Research
  Apr. 2007 - Mar. 2010
  TANAKA Saori; SUGAYA Tsutomu; MIYAJI Hirofumi
  To improve the bonding treatment of vertically fractured roots, we aimed to the newly cementum formation on a compound of resin and dentin powder, which may improve the binding between resin and cementum. In vitro, cytotoxicity and Micro Tensile Bond Strength (MTBS) test were assessed. It had little cytotoxicity and exhibited higher bond strength. In vivo, in some cases, we observed the newly cementum formation on a compound of resin and dentin powder. This experiment might exhibit a possibility of the newly cementum formation on a resin.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Coinvestigator, 19592191
• コラーゲンスポンジの骨髄間質細胞シートに対するスキャホールドとしての有効性とBMP-2併用による骨増生
  博士後期課程在学生研究助成
  Apr. 2008 - Mar. 2009
  井上加菜; 宮治裕史; 本郷哲也; 福田寛恵
  財団法人北海道大学クラーク記念財団, Coinvestigator, Competitive research funding
• Periodontal regeneration by BMP application to root surface and scaffold of periodontal ligament
  Grants-in-Aid for Scientific Research
  Apr. 2004 - Mar. 2008
  SUGAYA Tsutomu; DOMON Takanori; MIYAJI Hirofumi
  The aim of the first experiment was to evaluate the effect of different dentin conditionings followed rhBMP-2 application on dentin surface. Dentin blocks were demineralized with EDTA, tetracycline-HCI or citric acid solutions for 3 minutes, and were soaked with rhBMP-2 solution. Periodontal ligament cells were seeded on each dentin blocks, and ALP activity of attachment cells were assessed. The results showed that the groups of EDTA demineralization had higher ALP activity. In the second experiment, each dentin blocks were transplanted into palatal connective tissue of rats. The histological examinations showed that the groups of EDTA demineralization and rhBMP-2 application had significantly more hard tissue formation than any other groups. In the third experiment, dentin blocks were demineralized with EDTA and rhBMP-2 applied, and then implanted into palatal connective tissue of rats. Bisphosponate was administered to rats for osteoclast suppression. The results showed that hard tissue formation and dentin resorption were decreased. It suggested that coupling system between osteoblast and osteoclast may play an important role in hard tissue formation on dentin surface. The aim of fourth study was to evaluate periodontal regeneration in dehiscence model after EDTA demineralization, application of BMP-2 solution and collagen hydrogel to root dentin surfaces. New bone formation was significantly greater than any other group. Then, periodontal regeneration in 1-wall infrabony defects was evaluated. New bone and new cementum formation was significantly greater than in the control group. New cementum isolated from original cementum was frequently observed in the coronal area, and it might be formed by gingival connective tissue. These results suggest that new aspects of the regenerative process are introduced.
  It was concluded that the application of BMP-2 solution and collagen hydrogel to root dentin surface was conducive to periodontal tissue regeneration. This procedure may be effective regenerative therapy for large periodontal defects.
  Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 16390610
• BMPを歯根面に塗布しセメント質を再生させる新しい歯周組織再生療法の開発
  科学研究費助成事業
  Apr. 2004 - Mar. 2007
  宮治 裕史
  BMP-2を歯根象牙質表面に塗布し,セメント質を再生させる術式の検討を平成16年度より継続して行った.また平成18年度ではセメント質の再生量をさらに増加させるため,BMP-2を塗布し、さらにコラーゲンゲルを併用移植したモデルでの組織学的評価もおこなった.
  犬8頭の上下顎前臼歯歯根(128部位)の頬側歯肉歯槽粘膜を部分層弁で剥離し,骨膜を除去した後,高さ6mmの裂開状の骨欠損を作製し,露出した歯根面をルートプレーニングしてセメント質を除去した.次に歯根象牙質表面を24%EDTAにて3分間脱灰し4群に分け,BMP/Gel群ではrhBMP-2溶液(1000μg/ml)を歯根象牙質表面に塗布後さらにコラーゲンハイドロゲルを塗布,BMP群ではrhBMP-2のみを塗布,Gel群ではコラーゲンハイドロゲルのみを塗布,未塗布群では何も塗布せずに歯肉弁を復位縫合した.1,2,4,8週後,通法に従い脱灰薄切標本を作製,HE重染色を行い,組織学的観察および計測を行った.
  結果,8週後の歯槽骨新生率は,BMP/Gel群:87%,BMP群:65%,Gel群:41%,未塗布群:29%であり,BMP/Gel群は未塗布群(p<0.01),Gel群,BMP群(p<0.05)と比較して有意に大きかった.セメント質新生率はBMP/Gel群:45%,BMP群:48%,Gel群:39%,未塗布群:29%であり,BMP/Gel群はBMP群と比較して有意差は見られなかった.また,骨性癒着率はBMP/Gel群:6%,BMP群:9%,Gel群:0%,未塗布群:0%でいずれも少なかった.
  以上の結果からBMP-2塗布とコラーゲンゲルを併用移植したモデルでは,セメント質再生と同時に歯槽骨の再生が強く促進されることが明らかになり,新しい歯周組織再生療法として有効であることが明らかになった.
  日本学術振興会, 若手研究(B), 北海道大学, 16791310

• 抗菌・抗ウイルス性複合体
  Patent right, 宮治 裕史; 西田 絵利香; 浜本 朝子; ▲高▼田 礼人; 金本 佑生実; 郷田 隼; 小野 博信, 国立大学法人北海道大学, 株式会社日本触媒
  JP2022009483, 04 Mar. 2022
  特許第7591795号
  202403012920361154
• コラーゲン様ペプチド及びリン酸カルシウムの複合体並びにこれを含む生体組織修復材
  Patent right, 宮治裕史; 西田絵利香; 宮田さほり; 降籏友和, 国立大学法人北海道大学
  特願2018-217792, 20 Nov. 2018
  特開2020-083796, 04 Jun. 2020
  202003004214421614
• 亜鉛含有抗菌剤
  Patent right, 宮治 裕史; 西田 絵利香; 蔀 佳奈子; 浜本 朝子; 小野 博信; 郷田 隼, 国立大学法人北海道大学, 株式会社日本触媒
  特願2018-207370, 02 Nov. 2018
  特開2020-070277, 07 May 2020
  202003009450207978
• 抗菌剤組成物
  Patent right, 宮治 裕史; 西田 絵利香; 蔀 佳奈子; 小野 博信; 郷田 隼, 国立大学法人北海道大学, 株式会社日本触媒
  特願2018-207371, 02 Nov. 2018
  特開2020-070278, 07 May 2020
  202003009691473466
• 抗菌剤組成物
  Patent right, 宮治裕史; 西田絵利香; 蔀 佳奈子; 小野博信; 郷田 隼, 国立大学法人北海道大学, 株式会社日本触媒
  特願2018-207371, 02 Nov. 2018
  特開2020-070278, 07 May 2020
  特許第7177436号
  202003009691473466
• 亜鉛含有抗菌剤
  Patent right, 宮治裕史; 西田絵利香; 蔀 佳奈子; 浜本朝子; 小野博信; 郷田 隼, 国立大学法人北海道大学, 株式会社日本触媒
  特願2018-207370, 02 Nov. 2018
  特開2020-070277, 07 May 2020
  特許第7190132号
  202003009450207978
• 歯周病治療組成物およびそれを含む歯周病治療組成物キット
  Patent right, 宮治 裕史; 宮田 さほり; 眞弓 佳代子; 中塚 稔之; 高橋 啓至; 笠場 秀人, 株式会社松風
  特願2016-154288, 05 Aug. 2016
  特開2018-020986, 08 Feb. 2018
  201803010127572250
• 酸化グラフェンを用いた骨再生用又は皮膚再生用スキャフォールド
  Patent right, 宮治 裕史; 西田 絵利香; 川浪 雅光; 新開 妹井子; 三村 邦年, 国立大学法人北海道大学, 三菱瓦斯化学株式会社
  特願2015-037521, 27 Feb. 2015
  特開2016-158680, 05 Sep. 2016
  201603017565151755
• 抗菌性歯科用コーティング組成物
  Patent right, 宮治 裕史; 田中 佐織; 川浪 雅光; 新開 妹井子; 三村 邦年, 国立大学法人北海道大学, 三菱瓦斯化学株式会社
  特願2014-206954, 08 Oct. 2014
  特開2016-074636, 12 May 2016
  201603010624843920
• 骨再生材料及びその製造方法
  Patent right, 宮治 裕史; 川浪 雅光; 西村 浩之, 株式会社イノアック技術研究所, 国立大学法人北海道大学
  特願2010-156796, 09 Jul. 2010
  特開2012-016517, 26 Jan. 2012
  201203007191096156

• コロナを越えて・健康寿命を延ばそう！－医科と歯科の力で！！－
  23 Oct. 2022 - 23 Oct. 2022
  Informant
  東京保険医協会
  保険医協会健康まつり２０２２
• 北海道150年事業お口の健康公開講座．歯周病のこと，1から勉強してみませんか？－一生自分の歯で食事を楽しむためにー．
  17 Jun. 2018 - 17 Jun. 2018
  Lecturer
  ロート製薬 北海道新聞
  市民公開講座
• 歯の治療と研究の話
  26 Sep. 2017 - 26 Sep. 2017
  Lecturer
  札幌大谷高校
• 歯の病気と再生治療の研究
  21 Sep. 2016 - 21 Sep. 2016
  Lecturer
  札幌大谷高校
• 平成27年度北大病院実験教室
  27 Sep. 2015 - 27 Sep. 2015
  Lecturer, Planner, Organizing member
  宮治裕史
• 歯科治療に理工学の技術を使う・医工連携
  03 Sep. 2014 - 03 Sep. 2014
  Lecturer
  札幌大谷高校
• 平成26年度北大病院実験教室
  31 Aug. 2014 - 31 Aug. 2014
  Lecturer, Planner, Organizing member
  宮治裕史
• 歯の病気と再生治療の研究
  02 Sep. 2013 - 02 Sep. 2013
  Lecturer
  札幌大谷高校
• 平成25年度北大病院実験教室
  01 Sep. 2013 - 01 Sep. 2013
  Lecturer, Planner, Organizing member
  宮治裕史
• 平成24年度北大病院実験教室
  18 Nov. 2012 - 18 Nov. 2012
  Lecturer, Planner, Organizing member
  宮治裕史
• インプラントの予後-インプラントを長く持たせるための歯周病細菌コントロール-．
  11 Nov. 2012 - 11 Nov. 2012
  Lecturer
  北海道大学大学院歯学研究科
  市民公開特別講座

• 歯周病の基礎知識
  Apr. 2025
  Panasonic，WEBサイト, [Internet]
• 酸化グラフェンによる抗菌・抗ウイルス剤の基材定着メカニズムを解明～抗菌・抗ウイルス分野での応用を加速～
  19 Oct. 2022
  北海道大学病院
  プレスリリース, [Internet]
• 耐水性が高く透明な酸化グラフェン/抗菌・抗ウイルスコーティング
  May 2022
  Myself
  北海道大学
  研究シーズ集
  [Internet]
• 水で洗っても落ちない抗菌・抗ウイルス酸化グラフェン複合膜を開発～種々の菌，新型コロナウイルスを不活性化，水周り衛生に効果～
  14 Oct. 2021
  北海道大学病院
  プレスリリース, [Internet]
• 正しく理解しよう「歯周病」，歯周病の原因と対策
  02 Sep. 2020
  Myself
  ロート製薬，北海道テレビ放送（HTB）
  医TV・私たちの医療
  [Media report]
• 北海道150年事業お口の健康公開講座．歯周病のこと，1から勉強してみませんか？－一生自分の歯で食事を楽しむためにー．
  08 Aug. 2018
  Myself
  ロート製薬，北海道新聞
  北海道新聞朝刊，14面, [Paper]
• バイオ界面へのナノ物質修飾技術
  2017
  Myself
  北海道大学研究シーズ集
  [Internet]
• Collagen hydrogel scaffold and fibroblast growth factor-2 accelerate periodontal healing of class II
  24 Oct. 2016
  EurekAlert! Science News
  プレスリリース, [Internet]
• 生体材料のナノ・マイクロ表面改質技術
  2014
  北海道大学研究シーズ集, [Internet]