研究者基本情報

• 博士（農学）, 北海道大学, 2022年03月

• https://researchmap.jp/hiro-miura

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=202201005389814035

• 202201005389814035

• 消化管微生物
• 家畜栄養学
• 分子生態学
• 植物繊維分解
• ウシ
• ブタ
• 病原体検査

• ライフサイエンス, 動物生産科学

• 学士課程, 農学部
• 修士課程, 農学院
• 博士課程, 農学院

研究活動情報

• 2024年09月, 日本畜産学会第132回大会, 優秀発表賞
  ショットガンメタゲノム解析を利用した、繁殖母豚の生産成績とリンクする腸内細菌機能の探索
  三浦 広卓
• 2023年12月, 第26回 Hindgut Club Japanシンポジウム, 奨励賞
  生産成績が異なる農場間での繁殖母豚腸内細菌叢の糖質分解機能比較
  三浦 広卓
• 2019年10月, The 12th China-Japan-Korea Joint Symposium on Rumen Metabolism and Physiology, Best Oral Presentation Award
  Distribution of core rumen microbes and its relationship to feed efficiency in Japanese Black cattle
  Hiroto Miura

• Dietary 5-aminolevulinic acid modulates gut microbiota, reduces oxidative stress, and enhances immune status in weanling piglets: An 8-week exploratory study
  Shodai Ishikawa; Kiyonori Kawasaki; Kiminobu Yano; Kimiko Kazumura; Takamitsu Tsukahara; Shin Taniguchi; Hiroto Miura; Ryo Inoue
  Veterinary World, 355, 355, Veterinary World, 2026年01月30日, ［査読有り］
  研究論文（学術雑誌）, Background and Aim: Five-aminolevulinic acid (5-ALA), a precursor in heme biosynthesis, has gained attention as a functional feed additive due to its reported benefits on metabolism, redox balance, and immunity. Although supplementation in sows and broilers has demonstrated favorable physiological outcomes, its effects on the gut microbiota and immune–oxidative profiles of weanling piglets remain unclear. This study aimed to evaluate whether 5-ALA supplementation modifies fecal microbiota composition and influences oxidative stress and immune parameters in piglets during the post-weaning period.Materials and Methods: Twelve 28-day-old piglets were randomly allocated to a control or 5-ALA group (20 mg/kg feed) for 56 days. Body weight (BW), fecal samples, and blood samples were collected at 28, 56, and 84 days of age. Fecal microbiota was characterized by 16S rRNA sequencing (QIIME2). Oxidative stress and inflammatory markers in leukocytes (superoxide radical, hypochlorite ion) were quantified using a dual chemiluminescence/fluorescence system. Plasma malondialdehyde (MDA) and immunoglobulin G (IgG) were measured to assess systemic oxidative damage and humoral immunity. Statistical analyses included Permutational Multivariate Analysis of Variance, LEfSe, and Spearman correlations.Results: BW did not differ significantly between groups, although the 5-ALA group tended to be heavier at 56 days. β-diversity differed significantly between groups at 56 and 84 days. Six and eight bacterial genera were differentially abundant at 56 and 84 days, respectively; 5-ALA supplementation enriched short-chain fatty acids-associated genera such as Coprococcus, Prevotellaceae UCG-003, and Phascolarctobacterium. At 84 days, the 5-ALA group showed markedly lower leukocyte superoxide levels (~3.5-fold reduction; p < 0.05) and a tendency toward lower hypochlorite ion production. Plasma IgG concentration was approximately 1.5-fold higher in the 5-ALA group (p < 0.05). Multiple bacterial genera exhibited significant correlations with oxidative and immune markers.Conclusion: Dietary supplementation with 5-ALA altered the fecal microbiota and improved oxidative and immune status in weanling piglets, suggesting functional modulation of the gut–immune axis. Although exploratory and based on a small cohort, the findings warrant further controlled studies to validate dose–response effects and elucidate mechanistic pathways.Keywords: 5-aminolevulinic acid, antioxidant capacity, gut microbiota, immune response, piglets, oxidative stress, weaning stress, swine nutrition.
• Investigation of Metabolites in Feces and Plasma Associated with the Number of Piglets Weaned per Sow per Year
  Takamitsu Tsukahara; Hiroto Miura; Takahiro Kawase; Shu Yoshimura; Yoshihiro Mizukami; Yoshihiro Yahara; Kikuto Fukuta; Ryo Inoue
  Metabolites, 15, 11, 683, 683, MDPI AG, 2025年10月22日, ［査読有り］
  研究論文（学術雑誌）, Background: Sow reproductive performance is a critical parameter for the productivity of commercial pig farms. Gut microbiota is associated with performance in sow reproduction. At least, under healthy conditions, microbial metabolites from the gut microbiota are considered major contributors to host physiological regulation and productivity. However, information on the differences in gut-derived metabolites related to the sow reproductive performance remain scarce. Our aim was to investigate the relationship between the reproductive performance and microbial metabolite levels in sow’s feces and plasma. Methods: We selected four commercial farms: two with high- (group H) and two with low-reproductive performance (group L). Sows had their feces and blood collected. Results: Except for the iso-butyrate concentration, fecal short-chain fatty acid concentrations remained unchanged between groups. Among intestinal putrefactive metabolites, the indole concentration was higher (p < 0.05) in group H. The concentrations of plasma metabolites p-cresyl sulfate, p-cresyl glucuronide and trimethylamine N-oxide (TMAO) were higher (p < 0.05) in group L than in group H, while the opposite was true for the acetate concentration (p < 0.05). Among plasma biochemicals, tumor necrosis factor (TNF)-alpha and potassium concentrations were higher (p < 0.05) in group L. Conclusions: Blood metabolites, especially gut microbiota-derived metabolites, seemed to be associated with the performance related to sow reproduction. Particularly, harmful metabolites such as p-cresyl glucuronide, p-cresyl sulfate and TMAO were of importance, because they are potentially inflammation factors. In fact, TNF-alpha was stimulated in group L. According to our results, we estimated that p-cresyl glucuronide, p-cresyl sulfate, TMAO and TNF-alpha could be useful physiological indicators to understand sow reproductive performance.
• Assessment of Porcine Endogenous Retrovirus Infectivity to Human Cells Using Flowcytometric Isolation After Co-Culture
  Hiroto Miura; Haruka Uryu; Shodai Ishikawa; Takamitsu Tsukahara; Hiroshi Nagashima; Ryo Inoue
  Xenotransplantation, 32, 5, e70073, 2025年09月, ［査読有り］, ［筆頭著者］
• Effects of Blackcurrant Extract and Partially Hydrolyzed Guar Gum Intake on Gut Dysbiosis in Male University Rugby Players
  Hiroto Miura; Machi Oda; Kanako Abe; Hiromi Ikeda; Mami Fujibayashi; Naoko Oda; Tomohiro Segawa; Aya Abe; Natsumi Ueta; Takamitsu Tsukahara; Tomohisa Takagi; Yuji Naito; Ryo Inoue
  Microorganisms, 13, 7, 1561, 1561, MDPI AG, 2025年07月02日, ［査読有り］, ［筆頭著者］
  研究論文（学術雑誌）, Our previous study reported that male university rugby players tended to have a gut with a dysbiotic environment, characterized by abundant pathobiont bacteria and an accumulation of succinate, when compared with age-matched, non-rugby playing healthy males. In the present study, we conducted a randomized, double-blinded, placebo-controlled experiment to evaluate the potential of blackcurrant extract and/or partially hydrolyzed guar gum (PHGG) to improve the gut environment of university rugby players. Participants were supplemented with blackcurrant extract and/or PHGG or a placebo for 4 weeks. Beneficial gut bacteria such as Megasphaera spp. tended to increase (p < 0.10) and Bifidobacterium spp. increased (p < 0.05) with the intake of blackcurrant extract and/or PHGG. A subgroup analysis further indicated that, unlike in those with a eubiotic gut environment, the dietary supplements also increased the number of beneficial gut bacteria such as Phascolarctobacterium spp. (p < 0.10) and Faecalibacterium spp. (p < 0.10) and fecal SCFA concentrations (p < 0.05) in participants with a possible dysbiotic gut environment. However, a synergistic effect between blackcurrant extract and PHGG was not clearly observed. Although further investigation is recommended, it was concluded that blackcurrant extract and PHGG can at least be used as functional materials to improve gut dysbiosis in university rugby players.
• Partially hydrolyzed guar gum ingestion suppresses atopic dermatitis-like symptoms through prebiotic effect in mice
  So Morishima; Aya Abe; Saki Okamoto; Mahendra P. Kapoor; Shiho Matsuura; Kenji Kuriya; Makoto Ozeki; Masahiro Nishio; Hiroto Miura; Ryo Inoue
  Journal of Clinical Biochemistry and Nutrition, The Society for Free Radical Research Japan, 2025年05月, ［査読有り］
  研究論文（学術雑誌）
• Causal Association Between the Mucosal and Luminal Microbiotas from the Gastrointestinal Tract of Weaned Piglets Using Bayesian Network
  Shu Yoshimura; Takamitsu Tsukahara; Toru Takahashi; Hiroto Miura; So Morishima; Masaaki Kise; Jiye Shin; Yoshihiro Yahara; Ryo Inoue
  Microorganisms, 13, 2, 256, 256, MDPI AG, 2025年01月24日, ［査読有り］
  研究論文（学術雑誌）, The aim of this study was to investigate the microbiota composition and its potential interactions across seven gut locations (stomachs, jejuna, ilea, ceca, proximal colons, distal colons, and recta) in weaned pigs to identify key influencing microbiotas. To compare between microbiota compositions, 16S rRNA gene amplicon sequencing was performed. Six 70-day-old healthy crossbred (Duroc × Large White × Landrace) piglets were introduced as donors. A Bayesian network (BN) was used to examine the directional interactions among the microbiotas evaluated (seven mucosal and seven digesta microbiotas). Based on edge connectivity frequency, the microbiota in jejunal mucosa was the central hub node, influencing other microbiotas, especially the mucosal microbiotas of the ileum, cecum, distal colon, and rectum. The jejunal mucosa was dominated by Prevotella and lactobacilli, both recognized for their contributions to pig health. Among Prevotella, Prevotella copri and Prevotella sp. were predominant in jejunal mucosa (4.6% and 2.9%, respectively). Lactobacilli, including eight distinct species, were distributed throughout the gastrointestinal tract. Notably, Ligilactobacillus salivarius and Lactobacillus amylovorus, known as immune-enhancing bacteria, were abundant in jejunal mucosa (1.0% and 0.8%) and digestas (0.9% and 19.2%), respectively. The BN identified rectal mucosa and digestas as two terminal nodes, influenced by upstream microbiotas in the gastrointestinal tract. This finding supports the link between fecal microbiota and pig productivity, as the fecal microbiota, closely resembling the rectal microbiota, reflects the conditions of the microbiota throughout the gastrointestinal tract.
• Characterization of the gut phageome of Japanese patients with ulcerative colitis under endoscopic remission
  Akinori Otsuki; Ryo Inoue; Takayuki Imai; Hiroto Miura; Atsushi Nishida; Osamu Inatomi; Akira Andoh
  Journal of Clinical Biochemistry and Nutrition, The Society for Free Radical Research Japan, 2025年, ［査読有り］
  研究論文（学術雑誌）
• The expression of intestinal Cyp2c55 is regulated by the microbiota and inflammation
  Adrian Hilman; Tetsu Sato; Bambang Dwi Wijatniko; So Fujimura; Katsushi Nakamura; Hiroto Miura; Ken Iwatsuki; Ryo Inoue; Takuya Suzuki
  The FASEB Journal, 38, 20, Wiley, 2024年10月, ［査読有り］
  研究論文（学術雑誌）, Abstract
  
  Although the mutualistic relationship between the intestinal microbiota and the human host is crucial for maintaining health, the underlying mechanisms of this relationship remain unclear. In the present study, aiming to elucidate the regulatory mechanisms governing the Cyp2c55 expression, which is predominantly observed in colonic tissues, germ‐free, antibiotic‐administered and colitic mice, as well as mouse colonoids, were used as experimental models. RNA sequencing showed comparable decreases in the colonic Cyp2c55 expression in germ‐free and antibiotic‐administered mice, when compared with that in specific pathogen‐free mice. Furthermore, administration of dextran sulfate sodium decreased the Cyp2c55 expression in colitic mice. For these mice, a Pearson correlation analysis also showed a positive correlation between the Cyp2c55 expression and unconjugated bile acids (BAs), including chenodeoxycholic, muricholic, deoxycholic, lithocholic, and ursodeoxycholic acids, as well as taurine (T)‐conjugated secondary BAs, including deoxycholic acid. Moreover, bacterial genera, such as Muribaculaceae and unclassified Lachnospiraceae, also exhibited a positive correlation with these BAs. While administration of an agonist of the pregnane X receptor (PXR) increased the Cyp2c55 expression in mouse colonoids, inflammatory cytokines decreased it. In conclusion, Cyp2c55 was highly expressed in the colonic epithelial cells of mice in a microbiota‐dependent manner. The underlying mechanism seemed to involve a BA‐mediated PXR activation. In addition, the colonic expression of Cyp2c55 was regulated by the inflammatory response. Although the physiological function of Cyp2c55 remains largely unidentified, our findings suggested that Cyp2c55 may play a role in the mutualistic interaction between the intestinal microbiota and the intestinal homeostasis.
• Whole-Genome Metagenomic Analysis of Functional Profiles in the Fecal Microbiome of Farmed Sows with Different Reproductive Performances
  Hiroto Miura; Takamitsu Tsukahara; Ryo Inoue
  Microorganisms, 12, 11, 2024年10月, ［査読有り］, ［筆頭著者］
• 異種移植における感染症リスクの検査 -日本における現状と課題-
  井上亮; 三浦広卓
  人工臓器, 52, 3, 245, 249, 2023年12月
• Development of a panel for detection of pathogens in xenotransplantation donor pigs
  Hikari Otabi; Hiroto Miura; Haruka Uryu; Rana Kobayashi‐Harada; Kanako Abe; Kazuaki Nakano; Kazuhiro Umeyama; Koki Hasegawa; Takamitsu Tsukahara; Hiroshi Nagashima; Ryo Inoue
  Xenotransplantation, Wiley, 2023年09月28日, ［査読有り］, ［筆頭著者］
  研究論文（学術雑誌）, Abstract
  
  There have been high expectations in recent years of using xenotransplantation and regenerative medicine to treat humans, and pigs have been utilized as the donor model. Pigs used for these clinical applications must be microbiologically safe, that is, free of infectious pathogens, to prevent infections not only in livestock, but also in humans. Currently, however, the full spectrum of pathogens that can infect to the human host or cause disease in transplanted porcine organs/cells has not been fully defined. In the present study, we thus aimed to develop a larger panel for the detection of pathogens that could potentially infect xenotransplantation donor pigs. Our newly developed panel, which consisted of 76 highly sensitive PCR detection assays, was able to detect 41 viruses, 1 protozoa, and a broad range of bacteria (by use of universal 16S rRNA primers). The applicability of this panel was validated using blood samples from uterectomy‐born piglets, and pathogens suspected to be vertically transmitted from sows to piglets were successfully detected. We estimate that, at least for viruses and bacteria, the number of target pathogens detected by the developed screening panel should suffice to meet the microbiological safety levels required worldwide for xenotransplantation and/or regenerative therapy. This panel provides greater diagnosis options to produce donor pigs so that it would render unnecessary to screen for all pathogens listed. Instead, the new panel could be utilized to detect only required pathogens within a given geographic range where the donor pigs for xenotransplantation have been and/or are being developed.
• Reduction of butyric acid-producing bacteria in the ileal mucosa-associated microbiota is associated with the history of abdominal surgery in patients with Crohn’s disease
  Osamu Handa; Hiroto Miura; Tingting Gu; Motoyasu Osawa; Hiroshi Matsumoto; Eiji Umegaki; Ryo Inoue; Yuji Naito; Akiko Shiotani
  Redox Report, 28, 1, Informa UK Limited, 2023年08月, ［査読有り］
  研究論文（学術雑誌）
• In vitro Effects of Cellulose Acetate on Fermentation Profiles, the Microbiome, and Gamma-aminobutyric Acid Production in Human Stool Cultures
  Hiroaki Yamada; Hiroto Miura; Yutaka Suzuki; Satoshi Koike; Shu Shimamoto; Yasuo Kobayashi
  Current Microbiology, 80, 9, Springer Science and Business Media LLC, 2023年07月, ［査読有り］
  研究論文（学術雑誌）
• Evaluation of post‐colostrum ingestion changes in the protein composition of peripheral blood of newborn piglets: A pilot study
  Ken R. Ito; Kiyonori Kawasaki; Hiroto Miura; Takamitsu Tsukahara; Ryo Inoue
  Animal Science Journal, 93, 1, e13783, Wiley, 2022年12月, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）, Putatively, colostral proteins are partly absorbed and transferred to blood circulation in newborn piglets, which suggests that colostrum ingestion alters the protein composition of their blood. Here, we conducted a pilot study to estimate the changes in the protein composition of piglet blood. Plasma collected from piglets pre- and post-ingestion of colostrum (PreC and PostC) was analyzed by shotgun proteomics. Proteins in colostrum were also analyzed. We identified 393 and 427 proteins in PreC and PostC plasma, respectively, and 596 colostral proteins. Whereas 202 unique proteins were identified in PostC, PreC and PostC commonly shared 225 proteins. By contrast, when compared with PreC, 54 proteins in PostC had their emPAI values increased >2-fold. Notably, using plasma samples collected from a separate experiment, the concentrations of growth differentiation factor 8 and haptoglobin were higher in PostC than in PreC, which was validated by ELISA. Approximately 60% of the uniquely identified or highly concentrated proteins in PostC were also found in colostrum, which were likely, at least partly, transferred from colostrum. The present study demonstrated that the protein composition of plasma of newborn piglets drastically changed post-colostrum ingestion, partly due to transfer of colostral proteins.
• Effect of Porcine Colostral Exosomes on T Cells in the Peripheral Blood of Suckling Piglets
  Hiroto Miura; Itsuki Jimbo; Machi Oda; Michiko Noguchi; Kiyonori Kawasaki; Mayuko Osada-Oka; Takamitsu Tsukahara; Ryo Inoue
  Animals, 12, 17, 2172, 2172, MDPI AG, 2022年08月24日, ［査読有り］, ［筆頭著者］, ［国際誌］
  英語, 研究論文（学術雑誌）, Growing evidence indicates that porcine colostral exosomes may contribute to the healthy development of piglets. Here, we evaluated in vitro the effect of porcine milk-derived exosomes, in particular colostral exosomes, on T cells in the peripheral blood of suckling piglets. A total of seven sows and thirteen suckling piglets were used. Peripheral blood mononuclear cells (PBMCs) from suckling piglets were cultured with or without milk-derived exosomes (control). Using flow cytometry, the proportion of each T cell subset in cultured PBMCs was analyzed three days post-incubation. PBMCs cultured with porcine colostral exosomes had a higher proportion of CD3+CD4−CD8+ T cells (cytotoxic T cells; Tc) than the control. However, exosomes induced no increase in the Tc cell population in PBMC whose endocytosis was inhibited. We further measured the concentrations of cytokines in the culture supernatant. Exosome-treated PBMCs had a higher cytokine IL-2 concentration than the control. The present study demonstrated that porcine colostral exosomes could increase the Tc cell proportion in the peripheral blood of suckling piglets, with the underlying mechanism believed to be the stimulation of IL-2 production in PBMCs via endocytosis. Moreover, our results suggested that porcine colostral exosomes were involved in the development of cellular immunity in suckling piglets.
• Expression of chemerin in intestinal mucosa of calves with comparable expression level with other antimicrobial proteins
  Yutaka Suzuki; Kanako Kubota; Satoshi Haga; Hideaki Hayashi; Mutsumi Oishi; Hiroto Miura; Sanggun Roh; Satoshi Koike; Yasuo Kobayashi
  Animal Science Journal, 93, 1, Wiley, 2022年06月, ［査読有り］
  研究論文（学術雑誌）
• Alterations in rumen microbiota via oral fiber administration during early life in dairy cows
  Heshan Kodithuwakku; Daiki Maruyama; Hisashi Owada; Yuto Watabe; Hiroto Miura; Yutaka Suzuki; Kazuo Hirano; Yasuo Kobayashi; Satoshi Koike
  Scientific Reports, 12, 1, Springer Science and Business Media LLC, 2022年06月, ［査読有り］
  研究論文（学術雑誌）, Abstract
  
  Bacterial colonization in the rumen of pre-weaned ruminants is important for their growth and post-weaning productivity. This study evaluated the effects of oral fiber administration during the pre-weaning period on the development of rumen microbiota from pre-weaning to the first lactation period. Twenty female calves were assigned to control and treatment groups (n = 10 each). Animals in both groups were reared using a standard feeding program throughout the experiment, except for oral fiber administration (50–100 g/day/animal) from 3 days of age until weaning for the treatment group. Rumen content was collected during the pre-weaning period, growing period, and after parturition. Amplicon sequencing of the 16S rRNA gene revealed that oral fiber administration facilitated the early establishment of mature rumen microbiota, including a relatively higher abundance of Prevotella, Shuttleworthia, Mitsuokella, and Selenomonas. The difference in the rumen microbial composition between the dietary groups was observed even 21 days after parturition, with a significantly higher average milk yield in the first 30 days of lactation. Therefore, oral fiber administration to calves during the pre-weaning period altered rumen microbiota, and its effect might be long-lasting until the first parturition.
• Application of MinION Amplicon Sequencing to Buccal Swab Samples for Improving Resolution and Throughput of Rumen Microbiota Analysis.
  Hiroto Miura; Masayuki Takeda; Megumi Yamaguchi; Yoshihisa Ohtani; Go Endo; Yasuhisa Masuda; Kaede Ito; Yoshio Nagura; Kunihiro Iwashita; Tomohiro Mitani; Yutaka Suzuki; Yasuo Kobayashi; Satoshi Koike
  Frontiers in microbiology, 13, 783058, 783058, 2022年03月, ［査読有り］, ［筆頭著者］
  英語, 研究論文（学術雑誌）, The Illumina MiSeq platform has been widely used as a standard method for studying the rumen microbiota. However, the low resolution of taxonomic identification is the only disadvantage of MiSeq amplicon sequencing, as it targets a part of the 16S rRNA gene. In the present study, we performed three experiments to establish a high-resolution and high-throughput rumen microbial profiling approach using a combination of MinION platform and buccal swab sample, which is a proxy for rumen contents. In experiment 1, rumen contents and buccal swab samples were collected simultaneously from cannulated cattle (n = 6) and used for microbiota analysis using three different analytical workflows: amplicon sequencing of the V3-V4 region of the 16S rRNA gene using MiSeq and amplicon sequencing of near full-length 16S rRNA gene using MinION or PacBio Sequel II. All reads derived from the MinION and PacBio platforms were classified at the species-level. In experiment 2, rumen fluid samples were collected from beef cattle (n = 28) and used for 16S rRNA gene amplicon sequencing using the MinION platform to evaluate this sequencing platform for rumen microbiota analysis. We confirmed that the MinION platform allowed species-level taxa assignment for the predominant bacterial groups, which were previously identified at the family- and genus-level using the MiSeq platform. In experiment 3, buccal swab samples were collected from beef cattle (n = 30) and used for 16S rRNA gene amplicon sequencing using the MinION platform to validate the applicability of a combination of the MinION platform and buccal swab samples for rumen microbiota analysis. The distribution of predominant bacterial taxa in the buccal swab samples was similar to that in the rumen samples observed in experiment 2. Based on these results, we concluded that the combination of the MinION platform and buccal swab samples may be potentially applied for rumen microbial analysis in large-scale studies.
• Effects of oral administration of timothy hay and psyllium on the growth performance and fecal microbiota of preweaning calves
  K. A.H.T. Kodithuwakku; H. Owada; H. Miura; D. Maruyama; K. Hirano; Y. Suzuki; Y. Kobayashi; S. Koike
  Journal of Dairy Science, 104, 12, 12472, 12485, 2021年12月, ［査読有り］
  研究論文（学術雑誌）
• Identification of the core rumen bacterial taxa and their population dynamics during the fattening period in Japanese Black cattle.
  Hiroto Miura; Takuya Hashimoto; Yukiko Kawanishi; Hiroki Kawauchi; Ryo Inoue; Noriaki Shoji; Kunihiko Saito; Mario Sekiya; Yosuke Saito; Jumpei Yasuda; Chiemi Yonezawa; Tetsushiro Endo; Hirotaka Kasuya; Yutaka Suzuki; Yasuo Kobayashi; Satoshi Koike
  Animal science journal = Nihon chikusan Gakkaiho, 92, 1, e13601, 2021年12月, ［査読有り］, ［筆頭著者］, ［国際誌］
  英語, 研究論文（学術雑誌）, The rumen microbiota comprises a vast range of bacterial taxa, which may affect the production of high-quality meat in Japanese Black cattle. The aim of this study was to identify core rumen microbiota in rumen fluid samples collected from 74 Japanese Black cattle raised under different dietary conditions using 16S rRNA gene amplicon sequencing. In the rumen of fattening Japanese Black cattle, 10 bacterial taxa, showing >1% average relative abundance and >95% prevalence, irrespective of the dietary conditions and the fattening periods, were identified as the core rumen bacterial taxa, which accounted for approximately 80% of the rumen microbiota in Japanese Black cattle. Additionally, population dynamics of the core rumen bacterial taxa revealed two distinct patterns: Prevotella spp. and unclassified Bacteroidales decreased in the mid-fattening period, whereas unclassified Clostridiales, unclassified Ruminococcaceae, Ruminococcus spp., and unclassified Christensenellaceae increased during the same period. Therefore, the present study reports the wide distribution of the core rumen bacterial taxa in Japanese Black cattle, and the complementary nature of the population dynamics of these core taxa, which may ensure stable rumen fermentation during the fattening period.
• Rumen microbiota and its relation to fermentation in lactose-fed calves
  S. Koike; M. Ueno; H. Miura; A. Saegusa; K. Inouchi; Y. Inabu; T. Sugino; L. L. Guan; M. Oba; Y. Kobayashi
  Journal of Dairy Science, 104, 10, 10744, 10752, 2021年10月, ［査読有り］
  研究論文（学術雑誌）
• Effect of trehalose supplementation in milk replacer on the incidence of diarrhea and fecal microbiota in preweaned calves.
  Hiroto Miura; Kazuhisa Mukai; Keigo Sudo; Satoshi Haga; Yutaka Suzuki; Yasuo Kobayashi; Satoshi Koike
  Journal of animal science, 99, 1, 2021年01月, ［査読有り］, ［筆頭著者］
  英語, 研究論文（学術雑誌）, Trehalose, a nonreducing disaccharide consisting of d-glucose with α,α-1,1 linkage, was evaluated as a functional material to improve the gut environment in preweaned calves. In experiment 1, 173 calves were divided into two groups; the trehalose group was fed trehalose at 30 g/animal/d with milk replacer during the suckling period, and the control group was fed nonsupplemented milk replacer. Medication frequency was lower in the trehalose group (P < 0.05). In experiment 2, calves (n = 20) were divided into two groups (control group [n = 10] and trehalose group [n = 10]) based on their body weight and reared under the same feeding regimens as in experiment 1. Fresh feces were collected from individual animals at the beginning of the trial (average age 11 d), 3 wk after trehalose feeding (experimental day 22), and 1 d before weaning, and the fecal score was recorded daily. Fecal samples were analyzed for fermentation parameters and microbiota. The fecal score was significantly lower in the trehalose group than in the control group in the early stage (at an age of 14 to 18 d; P < 0.05) of the suckling period. Calves fed trehalose tended to have a higher proportion of fecal butyrate on day 22 than calves in the control group (P = 0.08). Population sizes of Clostridium spp. were significantly lower (P = 0.036), whereas those of Dialister spp. and Eubacterium spp. tended to be higher in the feces of calves in the trehalose group on day 22 (P = 0.060 and P = 0.083). These observations indicate that trehalose feeding modulated the gut environment and partially contributed to the reduction in medication frequency observed in experiment 1.

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  井上亮; 三浦広卓, 薬剤業務に生きてはたらく！ 腸内細菌Q＆A④ 食品・食事のすすめ篇
  南山堂, 2024年05月, ［分担執筆］
• ピッグジャーナル 2024年4月号 特集 抗菌剤の使用を減らそう！
  三浦広卓; 井上亮, 豚も"腸活"する時代に ~腸内細菌叢の改善による健康・生産性向上の可能性~
  アニマルメディア社, 2024年04月, ［分担執筆］
• 養豚界 2023年3月号 特集 知っておきたい！ 豚の腸内細菌叢
  三浦広卓, 豚の健康・生産性における繊維分解菌の重要性～なぜ繁殖成績の良い母豚では腸内に繊維分解菌が多いのか～
  緑書房, 2023年03月, ［分担執筆］

• 母豚の繁殖成績とリンクする腸内繊維分解菌の探索
  三浦 広卓
  日本畜産学会第132回大会 関連学術集会JAMIRAシンポジウム, 2024年09月
  ［招待講演］
• PHGGによる乳牛ルーメン細菌叢と乳生産への影響
  三浦 広卓
  日本畜産学会第132回大会 ランチョンセミナー, 2024年09月
  ［招待講演］
• 若手研究者からみるルーメン研究－黒毛和種牛の生産性とリンクするルーメン細菌群の探索を題材として－
  三浦 広卓
  第55回ルーメン研究会シンポジウム, 2023年03月20日
  ［招待講演］

• ブタ腸内細菌叢が持つ繊維分解能の発達動態の解明と早期強化による繁殖成績向上の検証
  2026年04月 - 2029年03月
  三浦 広卓
  日本学術振興会 科学研究費助成事業 若手研究 若手研究, 研究代表者, 26K18178
• ブタの繁殖性に関わる繊維分解菌の同定とその特性に基づく増殖・活性促進素材の探索
  科学研究費助成事業 若手研究
  2023年04月01日 - 2026年03月31日
  三浦 広卓
  日本学術振興会, 若手研究, 摂南大学, 23K14070
• 黒毛和種牛の飼料効率とルーメン細菌叢のリンクとその機序の解明
  科学研究費助成事業 特別研究員奨励費
  2021年04月 - 2022年03月
  三浦 広卓
  本研究では、黒毛和種牛の生産性向上が求められるという背景のもと、本品種の飼料効率とリンクする第一胃共生細菌群（ルーメン細菌叢）の特定を行った。まず（1）黒毛和種牛のルーメン細菌叢に関する基盤情報として、本品種の飼料分解に重要な役割を担うコアルーメン細菌群の特定を行った。ついで（2）より簡便かつ高解像度なルーメン細菌叢解析手法を検討し、（3）本手法を用いて黒毛和種牛の飼料効率とルーメン細菌叢の関連を探索した。
  （1）飼料条件の異なる5つの牧場で飼養された黒毛和種牛74頭から、肥育前期、中期および後期にルーメン内容物を経口採取し、細菌叢解析を行なった。飼料条件や肥育ステージを問わず高密度で分布する10細菌群を、黒毛和種牛ルーメン内での飼料分解・発酵に重要な役割を担うコアルーメン細菌群として特定した。
  （2）ルーメン内容物の代替である反芻残渣と小型ロングリードシーケンサーMinION（Oxford Nanopore社）の組み合わせによって、ルーメン細菌叢解析の簡便化・高解像度化を実現した。
  （3）同一の農場で飼養された黒毛和種牛83頭を肥育期間中の飼料効率（飼料摂取量に対する増体量）に基づいてグルーピングし、（2）で確立した手法を用いてルーメン細菌叢を比較解析した。その結果、肥育中期において、飼料効率の異なる牛群間でコアルーメン細菌群の分布動態が異なった。また、飼料効率の低い牛群でのみ、乳酸利用に関わる細菌群の減少が確認された。
  以上、本研究では黒毛和種牛の飼料効率とリンクする細菌群を特定した。本品種のルーメン内で重要な役割を担うコアルーメン細菌群の分布動態がルーメン発酵、ひいては宿主の飼料効率に影響することを示唆した。加えて、簡便かつ高解像度なルーメン細菌叢解析手法を応用することで、ルーメン内で乳酸代謝に関係する細菌群の減少が黒毛和種牛の飼料効率低下を導く可能性を明らかにした。
  日本学術振興会, 特別研究員奨励費, 北海道大学, 21J13430