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西田　絵利香 (ニシダ　エリカ)

歯学研究院　口腔医学部門　口腔健康科学分野助教
北海道大学病院助教

研究者基本情報

■ 学位
  • 博士（歯学）, 北海道大学, 2016年03月
■ URL
researchmap URLホームページURL■ ID 各種
研究者番号
  • 50779882
J-Global ID■ 研究キーワード・分野
研究分野
  • ライフサイエンス, 保存治療系歯学
■ 担当教育組織

経歴

■ 経歴
学歴
  • 北海道大学大学院, 歯学研究院, 日本国

研究活動情報

■ 論文
  • Zinc-addition route as a key parameter for forming an antibacterial apatite layer on dentin by a laser-assisted biomimetic process
    Kazuo Onuma; Miyabi Makino; Maki Nakamura; Erika Nishida; Kanako Shitomi; Hirofumi Miyaji; Ayako Oyane
    Surfaces and Interfaces, 2026年09月
    研究論文（学術雑誌）
  • Antibacterial crystalline bilayers constructed on dentin substrates via laser-assisted mineralization: characterization and functional implications
    Kazuo Onuma; Miyabi Makino; Maki Nakamura; Erika Nishida; Kanako Shitomi; Saori Tanaka; Hirofumi Miyaji; Ayako Oyane
    Applied Surface Science, 2025年12月
    研究論文（学術雑誌）
  • Sema3A-and ascorbate-functionalized apatite-collagen scaffolds for enhanced bone regeneration
    Kaushita Banerjee; Hanae Ishii; Ayako Oyane; Maki Nakamura; Tomoya Inose; Erika Nishida; Kari Tsukita; Tomoka Hasegawa; Hirofumi Miyaji
    MRS COMMUNICATIONS, 2025年08月21日
    英語, 研究論文（学術雑誌）
  • Integration of dual drugs into a collagen scaffold by a combination of apatite coating and impregnation with apatite particles for periodontal regeneration.
    Kaushita Banerjee; Ayako Oyane; Maki Nakamura; Tomoya Inose; Erika Nishida; Kanako Shitomi; Hirofumi Miyaji
    RSC advances, 15, 24, 19480, 19488, 2025年06月04日, ［国際誌］
    英語, 研究論文（学術雑誌）, Bioresorbable porous scaffolds capable of promoting osteoregeneration while preventing bacterial infection are needed for regenerative periodontal therapy. Previously, a porous collagen sponge coated with low-crystalline apatite has been shown to possess superior bioresorption and osteogenic properties compared to the uncoated sponge. In this study, we integrated osteogenic and antibacterial dual drugs into the sponge utilizing two types of apatite matrices to achieve further functionalization. First, the collagen sponge was coated with apatite loaded with an osteogenic drug, l-ascorbic acid 2-phosphate (AS), using a metastable supersaturated calcium phosphate (CaP) solution supplemented with AS. Second, the coated sponge was impregnated with apatite particles loaded with an antibacterial drug, ciprofloxacin (CF), which were fabricated using a labile supersaturated CaP solution supplemented with CF. The resulting dual drug-immobilized sponge demonstrated biological activities arising from both AS and CF; it enhanced proliferation of osteoblastic MC3T3-E1 cells and exhibited antibacterial activity against the oral bacterium Actinomyces naeslundii. The proposed technique to fabricate multifunctional scaffolds would offer a solution to provide more effective, patient-tailored regenerative periodontal therapy.
  • 酸化グラフェン超薄膜と口腔洗浄含嗽剤による抗菌性耐水コーティング
    西田 絵利香; 金本 佑生実; 石井 花英; 郷田 隼; 宮治 裕史
    日本歯科保存学雑誌, 68, 3, 103, 112, (NPO)日本歯科保存学会, 2025年06月
    日本語
  • 【抗菌性・抗ウイルス性バイオマテリアル アップデート】酸化グラフェン超薄膜の導入による抗菌剤の基材定着性の向上
    宮治 裕史; 西田 絵利香; 郷田 隼; 川崎 英也
    バイオマテリアル-生体材料-, 43, 2, 152, 157, 日本バイオマテリアル学会, 2025年04月
    日本語
  • Fluoride-incorporated apatite coating on resin-based composite via laser-assisted rapid pseudo-biomineralization
    Nandha Kumar Ponnusamy; Ayako Oyane; Maki Nakamura; Tomoya Inose; Kazuo Onuma; Erika Nishida; Hirofumi Miyaji
    Applied Surface Science, 2025年02月, ［査読有り］
    研究論文（学術雑誌）
  • 酸化グラフェン超薄膜と口腔洗浄含嗽剤による抗菌性耐水コーティング
    西田絵利香; 金本佑生実; 石井花英; 郷田隼; 宮治裕史
    日本歯科保存学雑誌(Web), 68, 3, 2025年
  • Ultrafast coating of fluoride-substituted hydroxyapatite layers on teeth by laser-assisted crystallization: Comparison of surface structure and composition among enamel, dentin, and cementum
    Kazuo Onuma; Miyabi Makino; Ikuko Sakamaki; Maki Nakamura; Erika Nishida; Saori Tanaka; Hirofumi Miyaji; Ayako Oyane
    Applied Surface Science, 674, 2024年11月15日, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Fabrication of Ciprofloxacin-Immobilized Calcium Phosphate Particles for Dental Drug Delivery.
    Aniruddha Pal; Ayako Oyane; Tomoya Inose; Maki Nakamura; Erika Nishida; Hirofumi Miyaji
    Materials (Basel, Switzerland), 17, 9, 2024年04月26日, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, Calcium phosphate (CaP) particles immobilizing antibacterial agents have the potential to be used as dental disinfectants. In this study, we fabricated CaP particles with immobilized ciprofloxacin (CF), a commonly prescribed antibacterial agent, via a coprecipitation process using a supersaturated CaP solution. As the aging time in the coprecipitation process increased from 2 to 24 h, the CaP phase in the resulting particles transformed from amorphous to low-crystalline hydroxyapatite, and their Ca/P elemental ratio, yield, and CF content increased. Despite the higher CF content, the particles aged for 24 h displayed a slower release of CF in a physiological salt solution, most likely owing to their crystallized matrix (less soluble hydroxyapatite), than those aged for 2 h, whose matrix was amorphous CaP. Both particles exhibited antibacterial and antibiofilm activities along with an acid-neutralizing effect against the major oral bacteria, Streptococcus mutans, Porphyromonas gingivalis, and Actinomyces naeslundii, in a dose-dependent manner, although their dose-response relationship was slightly different. The aging time in the coprecipitation process was identified as a governing factor affecting the physicochemical properties of the resulting CF-immobilized CaP particles and their functionality as a dental disinfectant.
  • Fluoride-Incorporated Apatite Coating on Collagen Sponge as a Carrier for Basic Fibroblast Growth Factor.
    Aniruddha Pal; Ayako Oyane; Maki Nakamura; Kenji Koga; Erika Nishida; Hirofumi Miyaji
    International journal of molecular sciences, 25, 3, 2024年01月25日, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, Coating layers consisting of a crystalline apatite matrix with immobilized basic fibroblast growth factor (bFGF) can release bFGF, thereby enhancing bone regeneration depending on their bFGF content. We hypothesized that the incorporation of fluoride ions into apatite crystals would enable the tailored release of bFGF from the coating layer depending on the layer's fluoride content. In the present study, coating layers consisting of fluoride-incorporated apatite (FAp) crystals with immobilized bFGF were coated on a porous collagen sponge by a precursor-assisted biomimetic process using supersaturated calcium phosphate solutions with various fluoride concentrations. The fluoride content in the coating layer increased with the increasing fluoride concentration of the supersaturated solution. The increased fluoride content in the coating layer reduced its solubility and suppressed the burst release of bFGF from the coated sponge into a physiological salt solution. The bFGF release was caused by the partial dissolution of the coating layer and, thus, accompanied by the fluoride release. The concentrations of released bFGF and fluoride were controlled within the estimated effective ranges in enhancing bone regeneration. These findings provide useful design guidelines for the construction of a mineralized, bFGF-releasing collagen scaffold that would be beneficial for bone tissue engineering, although further in vitro and in vivo studies are warranted.
  • Water-resistant antibacterial properties of a graphene oxide/cetylpyridinium chloride complex formed on medical gauze fibers.
    Yukimi Kanemoto; Hirofumi Miyaji; Erika Nishida; Asako Hamamoto; Tsutomu Sugaya; Syun Gohda; Hironobu Ono
    Journal of oral biosciences, 65, 2, 202, 205, 2023年06月, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, OBJECTIVES: Graphene oxide (GO) is a nanocarbon material with a high aspect ratio (width:thickness) and abundant anionic functional groups on its surface. In this study, we attached GO to the surface of medical gauze fibers, constructed a complex with a cationic surface active agent (CSAA), and demonstrated that the treated gauze exhibits antibacterial activity even after rinsing with water. METHODS: Medical gauze was immersed in GO dispersion (0.001%, 0.01%, and 0.1%), rinsed with water, dried, and subjected to the Raman spectroscopy analysis. Subsequently, the gauze treated with 0.001% GO dispersion was immersed in 0.1% cetylpyridinium chloride (CPC) solution, immediately rinsed with water, and dried. Untreated, GO-only, and CPC-only gauzes were prepared for comparison. Each gauze was placed in a culture well, seeded with Escherichia coli or Actinomyces naeslundii, and turbidity was measured after 24 h of incubation. RESULTS: The Raman spectroscopy analysis of the gauze after immersion and rinsing showed a G band peak, indicating that GO remained on the surface of the gauze. The turbidity measurements indicated that GO/CPC-treated gauze (GO-treated and rinsed, followed by CPC-treatment and rinsing) significantly decreased turbidity compared to the other gauzes (P < 0.05), suggesting that the GO/CPC complex remained on the gauze fibers even after water rinsing and showed antibacterial activity. CONCLUSIONS: The GO/CPC complex imparts water-resistant antibacterial properties to gauze and has the potential to be widely used for the antimicrobial treatment of clothes.
  • 酸化亜鉛ユージノール系ペーストタイプ根管充填シーラーの材料特性ならびに生体親和性評価
    加藤 昭人; 宮治 裕史; 金本 佑生実; 蔀 佳奈子; 岡本 一絵; 吉野 友都; 浜本 朝子; 西田 絵利香; 菅谷 勉; 田中 佐織
    日本歯科保存学雑誌, 66, 2, 114, 123, (NPO)日本歯科保存学会, 2023年04月
    日本語
  • Periodontal tissue regeneration by recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles.
    Yuto Yoshino; Hirofumi Miyaji; Erika Nishida; Yukimi Kanemoto; Asako Hamamoto; Akihito Kato; Tsutomu Sugaya; Tsukasa Akasaka
    Journal of oral biosciences, 65, 1, 62, 71, 2023年03月, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, OBJECTIVES: Recombinant human collagen peptide (RCP) is a recombinantly created xeno-free biomaterial enriched in arginine-glycine-aspartic acid sequences with good processability whose use for regenerative medicine applications is under investigation. The biocompatibility and osteogenic ability of RCP granules combined with β-tricalcium phosphate (TCP) submicron particles (β-TCP/RCP) were recently demonstrated. In the present study, β-TCP/RCP was implanted into experimental periodontal tissue defects created in beagles to investigate its regenerative effects. METHODS: An RCP solution was lyophilized, granulated, and thermally cross-linked into particles approximately 1 mm in diameter. β-TCP dispersion (1 wt%; 500 μL) was added to 100 mg of RCP granules to form β-TCP/RCP. A three-walled intrabony defect (5 mm × 3 mm × 4 mm) was created on the mesial side of the mandibular first molar and filled with β-TCP/RCP. RESULTS: A micro-computed tomography image analysis performed at 8 weeks postoperative showed a significantly greater amount of new bone after β-TCP/RCP grafting (2.2-fold, P < 0.05) than after no grafting. Histological findings showed that the transplanted β-TCP/RCP induced active bone-like tissue formation including tartaric acid-resistant acid phosphatase- and OCN-positive cells as well as bioabsorbability. Ankylosis did not occur, and periostin-positive periodontal ligament-like tissue formation was observed. Histological measurements performed at 8 weeks postoperative revealed that β-TCP/RCP implantation formed 1.7-fold more bone-like tissue and 2.1-fold more periodontal ligament-like tissue than the control condition and significantly suppressed gingival recession and epithelial downgrowth (P < 0.05). CONCLUSIONS: β-TCP/RCP implantation promoted bone-like and periodontal ligament-like tissue formation, suggesting its efficacy as a periodontal tissue regenerative material.
  • Rose bengal-decorated rice husk-derived silica nanoparticles enhanced singlet oxygen generation for antimicrobial photodynamic inactivation.
    Nanase Mori; Hideya Kawasaki; Erika Nishida; Yukimi Kanemoto; Hirofumi Miyaji; Junko Umeda; Katsuyoshi Kondoh
    Journal of materials science, 58, 6, 2801, 2813, 2023年, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, UNLABELLED: Rice husks are well known for their high silica content, and the RH-derived silica nanoparticles (RH NPs) are amorphous and biocompatible; therefore, they are suitable raw materials for biomedical applications. In this study, rose bengal-impregnated rice husk nanoparticles (RB-RH NPs) were prepared for their potential photosensitization and 1O2 generation as antimicrobial photodynamic inactivation. RB is a halogen-xanthene type's photosensitizer showing high singlet oxygen efficiency, and the superior photophysical properties are desirable for RB in the antimicrobial photodynamic inactivation of bacteria. To enhance the binding of anionic RB to RH NPs, we conducted cationization for the RH NPs using polyethyleneimine (PEI). The control of the RB adsorption state on cationic PEI-modified RH NPs was essential for RB RH-NP photosensitizers to obtain efficient 1O2 generation. Minimizing RB aggregation allowed highly efficient 1O2 production from RB-RH NPs at the molar ratio of RB with the PEI, XRB/PEI. = 0.1. The RB-RH NPs have significant antimicrobial activity against Streptococcus mutans compared to free RB after white light irradiation. The RB-RH NP-based antimicrobial photodynamic inactivation can be employed effectively in treating Streptococcus mutans for dental applications. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10853-023-08194-z.
  • In vivo Assessment of Tissue Compatibility of Root Canal Sealer Containing Surface Pre-reacted Glass-ionomer Fillers
    Saori TANAKA; Hirofumi MIYAJI; Kayoko MAYUMI; Yukimi K ANEMOTO; Erika NISHIDA
    Operative Dentistry, Endodontology and Periodontology, 2, 1, 33, 39, 2022年12月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Sustained antibacterial coating with graphene oxide ultrathin film combined with cationic surface-active agents in a wet environment.
    Hirofumi Miyaji; Yukimi Kanemoto; Asako Hamamoto; Kanako Shitomi; Erika Nishida; Akihito Kato; Tsutomu Sugaya; Saori Tanaka; Natsuha Aikawa; Hideya Kawasaki; Syun Gohda; Hironobu Ono
    Scientific reports, 12, 1, 16721, 16721, 2022年10月18日, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, Antimicrobial surfactants contained in mouthrinse have excellent efficacy, but are not retained on the tooth surface (are rinsed away) due to their low water resistance and thus do not exhibit sustained antibacterial activity. We have developed a new coating method using graphene oxide (GO) that retains the surfactant on the tooth surface even after rinsing with water, thus providing a sustained antibacterial effect. Ultra-thin films of GO and an antimicrobial agent were prepared by (1) applying GO to the substrate surface, drying, and thoroughly rinsing with water to remove excess GO to form an ultrathin film (almost a monolayer, transparent) on the substrate surface, then (2) applying antimicrobial cationic surface active agents (CSAAs) on the GO film to form a composite coating film (GO/CSAA). GO/CSAA formation was verified by scanning electron microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy, and ζ-potential and contact angle measurements. GO/CSAA was effective at inhibiting the growth of oral pathogens for up to 7 days of storage in water, and antibacterial activity was recovered by reapplication of the CSAA. Antibacterial GO/CSAA films were also formed on a tooth substrate. The results suggest that GO/CSAA coatings are effective in preventing oral infections.
  • Fabrication and cell behavior assessment of antibacterial micro/nano-patterned chitosan films
    Erika NISHIDA; Hirofumi MIYAJI; Tsutomu SUGAYA; Tsukasa AKASAKA
    Nano Biomedicine, 14, 1, 1, 8, 2022年, ［査読有り］, ［筆頭著者］
    英語
  • Periodontal tissue engineering using an apatite/collagen scaffold obtained by a plasma- and precursor-assisted biomimetic process.
    Yukimi Kanemoto; Hirofumi Miyaji; Erika Nishida; Saori Miyata; Kayoko Mayumi; Yuto Yoshino; Akihito Kato; Tsutomu Sugaya; Tsukasa Akasaka; Arputharaj Joseph Nathanael; Syama Santhakumar; Ayako Oyane
    Journal of periodontal research, 57, 1, 205, 218, 2022年01月, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, BACKGROUND AND OBJECTIVES: In the treatment of severe periodontal destruction, there is a strong demand for advanced scaffolds that can regenerate periodontal tissues with adequate quality and quantity. Recently, we developed a plasma- and precursor-assisted biomimetic process by which a porous collagen scaffold (CS) could be coated with low-crystalline apatite. The apatite-coated collagen scaffold (Ap-CS) promotes cellular ingrowth within the scaffold compared to CS in rat subcutaneous tissue. In the present study, the osteogenic activity of Ap-CS was characterized by cell culture and rat skull augmentation tests. In addition, the periodontal tissue reconstruction with Ap-CS in a beagle dog was compared to that with CS. METHODS: The plasma- and precursor-assisted biomimetic process was applied to CS to obtain Ap-CS with a low-crystalline apatite coating. The effects of apatite coating on the scaffold characteristics (i.e., surface morphology, water absorption, Ca release, protein adsorption, and enzymatic degradation resistance) were assessed. Cyto-compatibility and the osteogenic properties of Ap-CS and CS were assessed in vitro using preosteoblastic MC3T3-E1 cells. In addition, we performed in vivo studies to evaluate bone augmentation and periodontal tissue reconstruction with Ap-CS and CS in a rat skull and canine furcation lesion, respectively. RESULTS: As previously reported, the plasma- and precursor-assisted biomimetic process generated a low-crystalline apatite layer with a nanoporous structure that uniformly covered the Ap-CS surface. Ap-CS showed significantly higher water absorption, Ca release, lysozyme adsorption, and collagenase resistance than CS. Cell culture experiments revealed that Ap-CS was superior to CS in promoting the osteoblastic differentiation of MC3T3-E1 cells while suppressing their proliferation. Additionally, Ap-CS significantly promoted (compared to CS) the augmentation of the rat skull bone and showed the potential to regenerate alveolar bone in a dog furcation defect. CONCLUSION: Ap-CS fabricated by the plasma- and precursor-assisted biomimetic process provided superior promotion of osteogenic differentiation and bone neoformation compared to CS.
  • Antibacterial coating of tooth surface with ion-releasing pre-reacted glass-ionomer (S-PRG) nanofillers.
    Kayoko Mayumi; Hirofumi Miyaji; Saori Miyata; Erika Nishida; Tomokazu Furihata; Yukimi Kanemoto; Tsutomu Sugaya; Kanako Shitomi; Tsukasa Akasaka
    Heliyon, 7, 2, e06147, 2021年02月, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, OBJECTIVES: Surface pre-reacted glass-ionomer (S-PRG) fillers release antibacterial borate and fluoride ions. We fabricated nanoscale S-PRG fillers (S-PRG nanofillers) for antibacterial coating of tooth surfaces and assessed the antibacterial effects of this coating in vitro. In addition, we creating a canine model of periodontitis to evaluate the effectiveness of S-PRG nanofiller application on tooth roots and improvement of periodontal parameters. METHODS: Human dentin blocks were coated with S-PRG nanofiller (average particle size: 0.48 μm) and then characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectrometer (EDX), and ion-releasing test. Antibacterial effects of dentin blocks coated with S-PRG nanofiller were examined using bacterial strains, Streptococcus mutans and Actinomyces naeslundii. Next, we created an experimental model of periodontitis in furcation of premolars of beagle dogs. Then, S-PRG nanofiller coating was applied onto exposed tooth root surfaces. Periodontal parameters, gingival index (GI), bleeding on probing (BOP), probing pocket depth (PPD), and clinical attachment level (CAL), were measured from baseline until 4 weeks. In addition, bone healing was radiographically and histologically examined. RESULTS: SEM and EDX revealed that S-PRG nanofillers uniformly covered the dentin surface after coating. Dentin blocks coated with S-PRG nanofiller showed ion-releasing property, bacterial growth inhibition, and sterilization effects. In the experimental periodontitis model, S-PRG nanofiller coating significantly reduced clinical inflammatory parameters, such as GI (P < 0.01) and BOP (P < 0.05), compared to uncoated samples. In addition, PPD and CAL significantly decreased by S-PRG nanofiller coating (2 weeks: P < 0.05; 3 and 4 weeks: P < 0.01), suggesting the improvement of periodontitis. Micro-CT and histology revealed that bone healing of furcation defects was enhanced by S-PRG nanofiller coating. CONCLUSION: S-PRG nanofiller coating provides antibacterial effects to tooth surfaces and improves clinical parameters of periodontitis.
  • Evaluation of antibacterial and cytocompatible properties of multiple-ion releasing zinc-fluoride glass nanoparticles.
    Erika Nishida; Hirofumi Miyaji; Kanako Shitomi; Tsutomu Sugaya; Tsukasa Akasaka
    Dental materials journal, 40, 1, 157, 164, 2021年01月31日, ［査読有り］, ［国内誌］
    英語, 研究論文（学術雑誌）, Zinc-fluoride glass nanoparticles (Zinc-F) release several ions, such as fluoride, zinc and calcium ions, through acid-base reactions. The aim of this study was to evaluate the antibacterial and cytotoxic properties of Zinc-F. Antibacterial tests showed that a Zinc-F eluting solution significantly reduced the turbidity and colony-forming units of Streptococcus mutans and Actinomyces naeslundii, compared to that of calcium-fluoroaluminosilicate glass nanoparticles without zinc ions. In live/dead staining, Zinc-F eluate significantly decreased green-stained bacterial cells, indicating live cells, compared with the control (no application). Human dentin coated with Zinc-F showed suppressed S. mutans and A. naeslundii biofilm formation. Additionally, Zinc-F eluate showed low cytotoxic effects in osteoblastic and fibroblastic cells. Therefore, our findings suggested that Zinc-F exhibits antibacterial and biocompatible properties through multiple-ion release.
  • Bone forming ability of recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles.
    Tomokazu Furihata; Hirofumi Miyaji; Erika Nishida; Akihito Kato; Saori Miyata; Kanako Shitomi; Kayoko Mayumi; Yukimi Kanemoto; Tsutomu Sugaya; Tsukasa Akasaka
    Journal of biomedical materials research. Part B, Applied biomaterials, 108, 7, 3033, 3044, 2020年10月, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, Recombinant human collagen peptide, developed based on human collagen type I, contains an arginyl-glycyl-aspartic acid (RGD)-rich motif to enhance cell behavior and is anticipated as a xeno-free polymer material for use in tissue engineering. We fabricated granules containing recombinant human collagen peptide (RCP) applied with beta-tricalcium phosphate fine particles (RCP/β-TCP) as bone filling scaffold material and assessed the bone forming ability of RCP/β-TCP. Recombinant peptide was thermal crosslinked and freeze-dried to prepare RCP. An aqueous dispersion of β-TCP fine particles was added to RCP to obtain RCP/β-TCP. Subsequently, RCP/β-TCP were characterized using scanning electron microscopy (SEM), energy dispersive X-ray spectrometry (EDX), and cell culture assessments. Furthermore, RCP/β-TCP were implanted into rat cranial bone defects for radiographic and histological evaluations. In SEM and EDX analyses of RCP/β-TCP, β-TCP particles dose-dependently covered the surface of RCP. Cell culture tests showed that RCP/β-TCP remarkably promoted proliferation and mRNA expression of various genes, such as integrin β1 and osteogenic markers, of osteoblastic MC3T3-E1 cells. Histomorphometric assessment at 4 weeks showed that RCP/β-TCP significantly promoted new skull bone formation compared to RCP (p < 0.05) and control (no application) (p < 0.01). Accordingly, these findings suggest RCP/β-TCP possess bone forming capability and would be beneficial for bone tissue engineering therapy.
  • Comparative biological assessments of endodontic root canal sealer containing surface pre-reacted glass-ionomer (S-PRG) filler or silica filler.
    Hirofumi Miyaji; Kayoko Mayumi; Saori Miyata; Erika Nishida; Kanako Shitomi; Asako Hamamoto; Saori Tanaka; Tsukasa Akasaka
    Dental materials journal, 39, 2, 287, 294, 2020年03月31日, ［査読有り］, ［国内誌］
    英語, 研究論文（学術雑誌）, Surface pre-reacted glass-ionomer (S-PRG) filler releases several ions, such as fluoride, borate and strontium ions, to exert bioactive effects. We fabricated an endodontic root canal sealer containing S-PRG fillers (S-PRG sealer) and then evaluated the antibacterial and anti-inflammatory properties of S-PRG sealer compared with sealer containing conventional silica fillers (silica sealer). Antibacterial tests showed that S-PRG sealer significantly reduced the turbidity of Enterococcus faecalis compared with silica sealer. Implantation of S-PRG or silica sealer blocks in rat subcutaneous tissue showed that S-PRG sealer decreased the proinflammatory response compared with silica sealer at 10 days post-implantation. In addition, immunostaining revealed that infiltration of CD68- and peroxidase-positive cells around the S-PRG sealer was significantly lower than that in silica sealer. Therefore, it was suggested that S-PRG sealer exhibits antibacterial and anti-inflammatory effects.
  • Human Dentin Coated with Silver Nanoclusters Exhibits Antibacterial Activity against Streptococcus mutans
    Shitomi Kanako; Miyaji Hirofumi; Miyata Saori; Nishida Erika; Mayumi Kayoko; Sugaya Tsutomu; Kawasaki Hideya
    Nano Biomedicine, 11, 1, 21, 28, ナノ・バイオメディカル学会, 2019年06月, ［査読有り］
    英語, 虫歯の病原菌であるStreptococcus mutansに対して審美的(象牙質変色予防)作用と抗菌的作用を持つ成分として、直径2nm未満の銀ナノクラスター(AgNC)を調製した。このAgNCはS.mutansの濁度と生存性を顕著に減少させるだけでなく、LIVE/DEAD染色法にて殺菌効果が認められた。このAgNCをヒト象牙質に適用すると、フッ化ジアミン銀を適用した場合と比べて象牙質の変色は認められず、AgNC-処理象牙質はS.mutansによるコロニー形成に対する阻害効果を示した。これらの結果から、AgNCは抗菌的・審美的基質として、歯科療法で有望と考えられた。
  • Calcium phosphate coating on dental composite resins by a laser-assisted biomimetic process.
    A Joseph Nathanael; Ayako Oyane; Maki Nakamura; Kenji Koga; Erika Nishida; Saori Tanaka; Hirofumi Miyaji
    Heliyon, 4, 8, e00734, 2018年08月, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, OBJECTIVES: Dental composite resins with better biocompatibility and osteoconductivity have been sought in endodontic treatments. This study aimed to develop a technique to produce the osteoconductive resin surfaces through calcium phosphate (CaP) coating using a laser-assisted biomimetic (LAB) process. METHODS: Light-cured, acrylic-based composite resins were used as substrates. The resin substrate was subjected to a LAB process comprising Nd:YAG pulsed laser irradiation in a supersaturated CaP solution. The LAB-processed substrate was immersed for 3 days in a simulated body fluid (SBF) for the preliminary osteoconductivity assessment. RESULTS: After irradiation for 30 min, the resin surfaces were partly coated with a newly formed CaP layer. The coating layer contained hydroxyapatite as the main crystalline phase and the coating coverage depended on the laser wavelength and the type of resin. The LAB-processed CaP-coated surface exhibited apatite-forming ability in SBF. CONCLUSIONS: LAB process is effective for CaP coating on light-cured dental composite resins and improving their osteoconductivity. CLINICAL SIGNIFICANCE: The LAB process is a potential new tool to create a cementum-like osteoconductive surface on dental composite resins.
  • Evaluation of Tissue Behavior on Three-dimensional Collagen Scaffold Coated with Carbon Nanotubes and β-tricalcium Phosphate Nanoparticles
    Hirofumi MIYAJI; Shusuke MURAKAMI; Erika NISHIDA; Tsukasa AKASAKA; Bunshi FUGETSU; Junko UMEDA; Katsuyoshi KONDOH; Tadashi IIZUKA; Tsutomu SUGAYA
    Journal of Oral Tissue Engineering, 15, 3, 123, 130, 2018年05月, ［査読有り］
  • Near-infrared Irradiation and Graphene Oxide Film Fabricated on Dentin Surface Exhibit Photothermal and Antibacterial Effects
    Nagao Keishi; Miyaji Hirofumi; Nishida Erika; Akasaka Tsukasa; Miyata Saori; Shitomi Kanako; Mayumi Kayoko; Kato Akihito; Sugaya Tsutomu
    Journal of Oral Hygiene & Health, 6, 1, 231, 231, OMICS International, 2018年01月10日, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Bone induction by α-tricalcium phosphate microparticle emulsion containing simvastatin
    Akito Tateyama; Akihito Kato; Hirofumi Miyaji; Erika Nishida; Yasuhiko Iwasaki; Syuji Fujii; Kohei Kawamoto; Kanako Shitomi; Tomokazu Furihata; Kayoko Mayumi; Tsutomu Sugaya
    Nano Biomedicine, 9, 2, 69, 76, National Institute of Informatics, 2018年, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog.
    Kohei Kawamoto; Hirofumi Miyaji; Erika Nishida; Saori Miyata; Akihito Kato; Akito Tateyama; Tomokazu Furihata; Kanako Shitomi; Toshihiko Iwanaga; Tsutomu Sugaya
    International journal of nanomedicine, 13, 2365, 2376, 2018年, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, INTRODUCTION: The 3-dimensional scaffold plays a key role in volume and quality of repair tissue in periodontal tissue engineering therapy. We fabricated a novel 3D collagen scaffold containing carbon-based 2-dimensional layered material, named graphene oxide (GO). The aim of this study was to characterize and assess GO scaffold for periodontal tissue healing of class II furcation defects in dog. MATERIALS AND METHODS: GO scaffolds were prepared by coating the surface of a 3D collagen sponge scaffold with GO dispersion. Scaffolds were characterized using cytotoxicity and tissue reactivity tests. In addition, GO scaffold was implanted into dog class II furcation defects and periodontal healing was investigated at 4 weeks postsurgery. RESULTS: GO scaffold exhibited low cytotoxicity and enhanced cellular ingrowth behavior and rat bone forming ability. In addition, GO scaffold stimulated healing of dog class II furcation defects. Periodontal attachment formation, including alveolar bone, periodontal ligament-like tissue, and cementum-like tissue, was significantly increased by GO scaffold implantation, compared with untreated scaffold. CONCLUSION: The results suggest that GO scaffold is biocompatible and possesses excellent bone and periodontal tissue formation ability. Therefore, GO scaffold would be beneficial for periodontal tissue engineering therapy.
  • Dose effects of beta-tricalcium phosphate nanoparticles on biocompatibility and bone conductive ability of three-dimensional collagen scaffolds.
    Shusuke Murakami; Hirofumi Miyaji; Erika Nishida; Kohei Kawamoto; Saori Miyata; Hiroko Takita; Tsukasa Akasaka; Bunshi Fugetsu; Toshihiko Iwanaga; Hiromi Hongo; Norio Amizuka; Tsutomu Sugaya; Masamitsu Kawanami
    Dental materials journal, 36, 5, 573, 583, 2017年09月26日, ［査読有り］, ［国内誌］
    英語, 研究論文（学術雑誌）, Three-dimensional collagen scaffolds coated with beta-tricalcium phosphate (β-TCP) nanoparticles reportedly exhibit good bioactivity and biodegradability. Dose effects of β-TCP nanoparticles on biocompatibility and bone forming ability were then examined. Collagen scaffold was applied with 1, 5, 10, and 25 wt% β-TCP nanoparticle dispersion and designated TCP1, TCP5, TCP10, and TCP25, respectively. Compressive strength, calcium ion release and enzyme resistance of scaffolds with β-TCP nanoparticles applied increased with β-TCP dose. TCP5 showed excellent cell-ingrowth behavior in rat subcutaneous tissue. When TCP10 was applied, osteoblastic cell proliferation and rat cranial bone augmentation were greater than for any other scaffold. The bone area of TCP10 was 7.7-fold greater than that of non-treated scaffold. In contrast, TCP25 consistently exhibited adverse biological effects. These results suggest that the application dose of β-TCP nanoparticles affects the scaffold bioproperties; consequently, the bone conductive ability of TCP10 was remarkable.
  • Antibacterial and cytotoxic effects of photoexcited Au clusters via blue high-power or white low-power light emitting diode irradiation
    Saori Miyata; Hirofumi Miyaji; Hideya Kawasaki; Erika Nishida; Kanako Shitomi; Tsukasa Akasaka; Saori Tanaka; Tadashi Iizuka; Tsutomu Sugaya
    Biology, Engineering and Medicine, 2, 4, 1, 4, Open Access Text, 2017年09月, ［査読有り］
    英語, 研究論文（学術雑誌）, The development of photosensitizers and light sources has enabled the use of antimicrobial photodynamic therapy (aPDT) in various dental therapies. In the presentstudy, we compared the antibacterial and cytotoxic effects of Au clusters photoexcited by blue and white LED irradiation. We fabricated novel photosensitizers,captopril-protected gold (Capt-Au) clusters and lysozyme-stabilized gold (Lyz-Au) clusters, for aPDT. Au clusters were then photoexcited by two kinds of lightsources, blue high-power and white low-power light-emitting diodes (LEDs). Since white LED contains a wide spectrum of light (400–750 nm), white LED wouldbe relevant for aPDT even if using a low-power source.The turbidity and viability of Streptococcus mutans were assessed following application of Capt-Au clusters (500 μg/mL) or Lyz-Au clusters (1,000 μg/mL) photoexcitedby a blue high-power LED (1,000 mW/cm2) or white low-power LED (80 mW/cm2). In addition, the cytotoxicity of Au clusters and LED irradiation was evaluatedin NIH3T3 and MC3T3-E1 cells.Au clusters photoexcited by the white low-power LED equally decreased the turbidity and viability of S. mutans compared with blue high-power LED. However,Au clusters photoexcited by white LED irradiation caused decreased cytotoxicity in mammalian cells compared with those photoexcited by blue LED irradiation. Inconclusion, white LEDs possess biosafe properties for aPDT using Au clusters.
  • In Vitro and in Vivo Analysis of Mineralized Collagen-Based Sponges Prepared by a Plasma- and Precursor-Assisted Biomimetic Process.
    A Joseph Nathanael; Ayako Oyane; Maki Nakamura; Ikuko Sakamaki; Erika Nishida; Yukimi Kanemoto; Hirofumi Miyaji
    ACS applied materials & interfaces, 9, 27, 22185, 22194, 2017年07月12日, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, Three-dimensional (3D) porous scaffolds for supporting cell adhesion and growth play a vital role in tissue engineering applications. In the present study, three different collagen-based 3D sponges were functionalized by apatite coating. The sponges were coated with apatite on their outer and inner surfaces while retaining their interconnecting pores. To achieve this, we employed a vacuum degassing system in our plasma- and precursor-assisted biomimetic process using a supersaturated calcium phosphate solution. The resulting apatite-coated sponges (mineralized sponges) showed better cell adhesion properties in vitro for osteoblast-like MC3T3-E1 cells compared to that of uncoated sponges. The three mineralized sponges were implanted in the subcutaneous tissue of rats. Upon histological evaluation after 10 days, the mineralized sponges showed cell in-growth rates that were approximately 4-fold greater than those of the untreated sponges without any notable inflammatory reactions. As these sponges are composed of clinically approved collagen-based frameworks and possess a 3D porous structure with a mineralized surface appropriate for cell adhesion and internalization, further in vitro and in vivo studies should be conducted regarding tissue engineering applications.
  • Antimicrobial photodynamic activity and cytocompatibility of Au25(Capt)18 clusters photoexcited by blue LED light irradiation.
    Saori Miyata; Hirofumi Miyaji; Hideya Kawasaki; Masaki Yamamoto; Erika Nishida; Hiroko Takita; Tsukasa Akasaka; Natsumi Ushijima; Toshihiko Iwanaga; Tsutomu Sugaya
    International journal of nanomedicine, 12, 2703, 2716, 2017年, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, Antimicrobial photodynamic therapy (aPDT) has beneficial effects in dental treatment. We applied captopril-protected gold (Au25(Capt)18) clusters as a novel photosensitizer for aPDT. Photoexcited Au clusters under light irradiation generated singlet oxygen (1O2). Accordingly, the antimicrobial and cytotoxic effects of Au25(Capt)18 clusters under dental blue light-emitting diode (LED) irradiation were evaluated. 1O2 generation of Au25(Capt)18 clusters under blue LED irradiation (420-460 nm) was detected by a methotrexate (MTX) probe. The antimicrobial effects of photoexcited Au clusters (0, 5, 50, and 500 μg/mL) on oral bacterial cells, such as Streptococcus mutans, Aggregatibacter actinomycetemcomitans, and Porphyromonas gingivalis, were assessed by morphological observations and bacterial growth experiments. Cytotoxicity testing of Au clusters and blue LED irradiation was then performed against NIH3T3 and MC3T3-E1 cells. In addition, the biological performance of Au clusters (500 μg/mL) was compared to an organic dye photosensitizer, methylene blue (MB; 10 and 100 μg/mL). We confirmed the 1O2 generation ability of Au25(Capt)18 clusters through the fluorescence spectra of oxidized MTX. Successful application of photoexcited Au clusters to aPDT was demonstrated by dose-dependent decreases in the turbidity of oral bacterial cells. Morphological observation revealed that application of Au clusters stimulated destruction of bacterial cell walls and inhibited biofilm formation. Aggregation of Au clusters around bacterial cells was fluorescently observed. However, photoexcited Au clusters did not negatively affect the adhesion, spreading, and proliferation of mammalian cells, particularly at lower doses. In addition, application of Au clusters demonstrated significantly better cytocompatibility compared to MB. We found that a combination of Au25(Capt)18 clusters and blue LED irradiation exhibited good antimicrobial effects through 1O2 generation and biosafe characteristics, which is desirable for aPDT in dentistry.
  • Periodontal tissue engineering by nano beta-tricalcium phosphate scaffold and fibroblast growth factor-2 in one-wall infrabony defects of dogs
    K. Ogawa; H. Miyaji; A. Kato; Y. Kosen; T. Momose; T. Yoshida; E. Nishida; S. Miyata; S. Murakami; H. Takita; B. Fugetsu; T. Sugaya; M. Kawanami
    JOURNAL OF PERIODONTAL RESEARCH, 51, 6, 758, 767, 2016年12月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • カーボンナノシート新素材『酸化グラフェン』の歯周病治療への展開
    宮治 裕史; 西田 絵利香
    日本歯周病学会会誌, 58, 2, 65, 71, (NPO)日本歯周病学会, 2016年07月, ［査読有り］
    日本語, 歯周病治療において、新しい骨補填材やティッシュエンジニアリング法が開発され、多面的に新技術が応用されつつある。グラファイトから生成されるカーボンナノシート「酸化グラフェン」は、安全性が高く優れた理工学的特性を有するとともに、抗菌性やドラッグデリバリー効果、培養細胞へのポジティブな効果が報告されている。酸化グラフェンについて説明し、酸化グラフェンの細胞活性への効果、酸化グラフェンのティッシュエンジニアリングへの応用、酸化グラフェンの抗菌性評価について述べた。
  • Modification of Composite Resin Surface with Carbon Nanotubes Enhances Cell Proliferation
    Saori TANAKA; Hirofumi MIYAJI; Erika NISHIDA; Kana INOUE; Saori MIYATA; Akihito KATO; Izumi KANAYAMA; Shusuke MURAKAMI; Kohei KAWAMOTO; Bunshi FUGETSU; Toru TANAKA; Hiroko TAKITA; Tadashi IIZUKA; Masamitsu KAWANAMI
    Nano Biomedicine, 8, 1, 27, 34, 2016年06月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Preparation of Chitosan-Gelatin Based Sponge Cross-Linked with GlcNAc for Bone Tissue Engineering
    Thitirat Chaochai; Hirofumi Miyaji; Takashi Yoshida; Erika Nishida; Tetsuya Furuike; Hiroshi Tamura
    Journal of Chitin and Chitosan Science, 4, 1, 1, 8, American Scientific Publishers, 2016年03月01日, ［査読有り］
    研究論文（学術雑誌）
  • β-三リン酸カルシウムナノ粒子配合コラーゲンスキャフォールドのイヌ抜歯窩骨形成促進効果
    加藤 昭人; 川浪 雅光; 宮治 裕史; 小川 幸佑; 百瀬 赳人; 西田 絵利香; 村上 秀輔; 吉田 崇; 田中 佐織; 菅谷 勉
    日本歯科保存学雑誌, 59, 4, 351, 358, 日本歯科保存学会, 2016年, ［査読有り］
    日本語,

     目的 : β-三リン酸カルシウム (β-TCP) は骨補塡材として臨床応用されているが, 吸収が遅いため組織置換に時間を要することや, 残留材料の汚染による不良な治癒発現の問題がある. そこでわれわれはナノ化技術を応用し, ナノサイズ化したβ-TCP粒子をコラーゲンスポンジに配合したβ-TCPナノ粒子配合コラーゲンスキャフォールドを創製した. このスキャフォールドをラット頭蓋骨に応用した研究では, 骨増生効果が認められ, またコラーゲンスポンジよりもすみやかに吸収されることが報告された. そこで本研究では, β-TCPナノ粒子配合コラーゲンスキャフォールドをイヌ抜歯窩へ埋植して骨形成効果を評価した.

     材料と方法 : β-TCP粒子 (平均粒径2.3μm) をナノ粒子に粉砕後, コール酸ナトリウムを分散剤として, β-TCPの水分散液を作製した. 次にコラーゲンスポンジを分散液に浸漬した後, 洗浄乾燥してβ-TCPスキャフォールドを作製した. ナノ粒子の付着状態を観察するために, スキャフォールド表面のSEM観察を行った. イヌ抜歯窩埋植試験はビーグル犬の上顎第一前臼歯抜歯窩に, 実験群ではβ-TCPナノ粒子配合コラーゲンスキャフォールド, 対照群ではコラーゲンスポンジの埋植を行い, 経時的なエックス線写真撮影による評価, ならびに2, 5週後における組織学的評価を行った.

     結果 : SEM観察においてコラーゲンスポンジ線維表面に100nm程度のβ-TCPナノ粒子が認められ, スポンジ内部は粒子で埋まることなく維持されていた. 抜歯窩埋植試験の結果, 実験群のエックス線写真では対照群と比較して不透過性の亢進が早期に認められた. 組織学的観察では, 2週の実験群で抜歯窩内に既存骨から連続する新生骨が多く認められ, スキャフォールド内部に多量の細胞や血管を観察した. 一方, 2週対照群の新生骨はわずかであった. 5週の実験群では抜歯窩内に残存スキャフォールドは認められず, 中心部まで新生骨を認めた. 対照群5週では新生骨が観察されたが, 一部の標本では抜歯窩中心部が結合組織と残存コラーゲンスポンジで占められていた. 2週における新生骨形成率は, 実験群が対照群に比べて約3倍以上と有意に大きく, 埋植材残存率は, 実験群が有意に小さかった.

     結論 : β-TCPナノ粒子配合コラーゲンスキャフォールドは良好な生体親和性・吸収性を示し, イヌ抜歯窩の骨形成を促進した.

  • Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket.
    Erika Nishida; Hirofumi Miyaji; Akihito Kato; Hiroko Takita; Toshihiko Iwanaga; Takehito Momose; Kosuke Ogawa; Shusuke Murakami; Tsutomu Sugaya; Masamitsu Kawanami
    International journal of nanomedicine, 11, 2265, 77, 2016年, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, Graphene oxide (GO) consisting of a carbon monolayer has been widely investigated for tissue engineering platforms because of its unique properties. For this study, we fabricated a GO-applied scaffold and assessed the cellular and tissue behaviors in the scaffold. A preclinical test was conducted to ascertain whether the GO scaffold promoted bone induction in dog tooth extraction sockets. For this study, GO scaffolds were prepared by coating the surface of a collagen sponge scaffold with 0.1 and 1 µg/mL GO dispersion. Scaffolds were characterized using scanning electron microscopy (SEM), physical testing, cell seeding, and rat subcutaneous implant testing. Then a GO scaffold was implanted into a dog tooth extraction socket. Histological observations were made at 2 weeks postsurgery. SEM observations show that GO attached to the surface of collagen scaffold struts. The GO scaffold exhibited an interconnected structure resembling that of control subjects. GO application improved the physical strength, enzyme resistance, and adsorption of calcium and proteins. Cytocompatibility tests showed that GO application significantly increased osteoblastic MC3T3-E1 cell proliferation. In addition, an assessment of rat subcutaneous tissue response revealed that implantation of 1 µg/mL GO scaffold stimulated cellular ingrowth behavior, suggesting that the GO scaffold exhibited good biocompatibility. The tissue ingrowth area and DNA contents of 1 µg/mL GO scaffold were, respectively, approximately 2.5-fold and 1.4-fold greater than those of the control. Particularly, the infiltration of ED2-positive (M2) macrophages and blood vessels were prominent in the GO scaffold. Dog bone-formation tests showed that 1 µg/mL GO scaffold implantation enhanced bone formation. New bone formation following GO scaffold implantation was enhanced fivefold compared to that in control subjects. These results suggest that GO was biocompatible and had high bone-formation capability for the scaffold. The GO scaffold is expected to be beneficial for bone tissue engineering therapy.
  • Collagen Hydrogel Scaffold and Fibroblast Growth Factor-2 Accelerate Periodontal Healing of Class II Furcation Defects in Dog.
    Takehito Momose; Hirofumi Miyaji; Akihito Kato; Kosuke Ogawa; Takashi Yoshida; Erika Nishida; Syusuke Murakami; Yuta Kosen; Tsutomu Sugaya; Masamitsu Kawanami
    The open dentistry journal, 10, 347, 59, 2016年, ［査読有り］, ［国際誌］
    英語, 研究論文（学術雑誌）, OBJECTIVE: Collagen hydrogel scaffold exhibits bio-safe properties and facilitates periodontal wound healing. However, regenerated tissue volume is insufficient. Fibroblast growth factor-2 (FGF2) up-regulates cell behaviors and subsequent wound healing. We evaluated whether periodontal wound healing is promoted by application of collagen hydrogel scaffold in combination with FGF2 in furcation defects in beagle dogs. METHODS: Collagen hydrogel was fabricated from bovine type I collagen with an ascorbate-copper ion cross-linking system. Collagen hydrogel was mingled with FGF2 and injected into sponge-form collagen. Subsequently, FGF2 (50 µg)/collagen hydrogel scaffold and collagen hydrogel scaffold alone were implanted into class II furcation defects in dogs. In addition, no implantation was performed as a control. Histometric parameters were assessed at 10 days and 4 weeks after surgery. RESULT: FGF2 application to scaffold promoted considerable cell and tissue ingrowth containing numerous cells and blood vessel-like structure at day 10. At 4 weeks, reconstruction of alveolar bone was stimulated by implantation of scaffold loaded with FGF2. Furthermore, periodontal attachment, consisting of cementum-like tissue, periodontal ligament-like tissue and Sharpey's fibers, was also repaired, indicating that FGF2-loaded scaffold guided self-assembly and then re-established the function of periodontal organs. Aberrant healing, such as ankylosis and root resorption, was not observed. CONCLUSION: FGF2-loaded collagen hydrogel scaffold possessed excellent biocompatibility and strongly promoted periodontal tissue engineering, including periodontal attachment re-organization.
  • Friction behavior of network-structured CNT coating on pure titanium plate
    Junko Umeda; Bunshi Fugetsu; Erika Nishida; Hirofumi Miyaji; Katsuyoshi Kondoh
    APPLIED SURFACE SCIENCE, 357, 721, 727, 2015年12月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Biological Response to Nanostructure of Carbon Nanotube/titanium Composite Surfaces
    Erika NISHIDA; Hirofumi MIYAJI; Junko UMEDA; Katsuyoshi KONDOH; Hiroko TAKITA; Izumi KANAYAMA; Saori TANAKA; Akihito KATO; Bunshi FUGETSU; Tsukasa AKASAKA; Masamitsu KAWANAMI
    Nano Biomedicine, 7, 1, 11, 20, 2015年06月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Bone augmentation using a highly porous PLGA/β-TCP scaffold containing fibroblast growth factor-2
    T. Yoshida; H. Miyaji; K. Otani; K. Inoue; K. Nakane; H. Nishimura; A. Ibara; A. Shimada; K. Ogawa; E. Nishida; T. Sugaya; L. Sun; B. Fugetsu; M. Kawanami
    J Periodontal Res, 50, 2, 265, 273, 2015年04月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Graphene oxide coating facilitates the bioactivity of scaffold material for tissue engineering
    Erika Nishida; Hirofumi Miyaji; Hiroko Takita; Izumi Kanayama; Maiko Tsuji; Tsukasa Akasaka; Tsutomu Sugaya; Ryuji Sakagami; Masamitsu Kawanami
    JAPANESE JOURNAL OF APPLIED PHYSICS, 53, 6, 06JD04, 2014年06月, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Comparative study of bioactivity of collagen scaffolds coated with graphene oxide and reduced graphene oxide
    Izumi Kanayama; Hirofumi Miyaji; Hiroko Takita; Erika Nishida; Maiko Tsuji; Bunshi Fugetsu; Ling Sun; Kana Inoue; A. Sako Ibara; Tsukasa Akasaka; Tsutomu Sugaya; Masamitsu Kawanami
    INTERNATIONAL JOURNAL OF NANOMEDICINE, 9, 3363, 3373, 2014年, ［査読有り］
    英語, 研究論文（学術雑誌）
  • Bone Augmentation in Rat by Highly Porous β-TCP Scaffolds with Different Open-cell Sizes in Combination with Fibroblast Growth Factor-2
    MIYAJI Hirofumi; YOKOYAMA Hiroyuki; KOSEN Yuta; NISHIMURA Hiroyuki; NAKANE Kazuyasu; TANAKA Saori; OTANI Kaori; INOUE Kana; IBARA Asako; KANAYAMA Izumi; YOSHIDA Takashi; OGAWA Kosuke; NISHIDA Erika; KAWANAMI Masamitsu
    Journal of Oral Tissue Engineering, 10, 3, 172, 181, 日本再生歯科医学会, 2013年, ［査読有り］
    英語, We prepared highly porous beta-tricalcium phosphate (β-TCP) scaffolds with different open-cell structure sizes. The aim of this study was to examine whether the open-cell size of the scaffold affected osteoinduction in combination with fibroblast growth factor-2 (FGF2) in rats. Polyurethane foam was immersed in β-TCP slurry and sintered in a furnace. Porous β-TCP scaffolds were prepared in three cell sizes (0.6, 0.4 and 0.3 mm) and characterized. Subsequently, each scaffold with FGF2 was implanted to rat cranial bone. Histomorphometric analyses were taken at 35 days post-surgery. The results showed that each β-TCP scaffold exhibited fully interconnected porosity, and frequently allowed bone tissue ingrowth. The 0.4-mm cell sized scaffold significantly promoted bone augmentation compared to the 0.3-mm type. Resorption of the β-TCP scaffold of 0.4-mm cell size was frequently accelerated. In conclusion, FGF2-loaded β-TCP scaffolds with 0.4-mm cell size would be effective for bone tissue engineering.
  • Osteoinductivity and biodegradability of collagen scaffold coated with Nano-β-TCP and fibroblast groueth factor 2
    Asako Ibara; Hirofumi Miyaji; Bunshi Fugetsu; Erika Nishida; Hiroko Takita; Saori Tanaka; Tsutomu Sugaya; Masamitsu Kawanami
    J Nanomater, 2013年, ［査読有り］
    英語, 研究論文（学術雑誌）
■ その他活動・業績
■ 主な担当授業
  • フロンティア基礎科目, 2024年, 学士課程, 歯学部
■ 共同研究・競争的資金等の研究課題
  • 人工エナメルその場合成による異種接合界面の精密制御と医用展開
    科学研究費助成事業
    2026年06月26日 - 2029年03月31日
    大矢根 綾子; 中村 真紀; 秋山 陽久; 島本 一正; 宮治 裕史; 西田 絵利香
    日本学術振興会, 挑戦的研究(開拓), 国立研究開発法人産業技術総合研究所, 26K21970
  • レーザーで軟化象牙質をアパタイトに置換する－根面う蝕治療法の新規開発
    科学研究費助成事業
    2025年04月01日 - 2028年03月31日
    宮治 裕史; 大矢根 綾子; 松田 康裕; 西田 絵利香; 蔀 佳奈子
    日本学術振興会, 基盤研究(B), 北海道大学, 25K02808
  • LABプロセスを用いたインプラント周囲炎の革新的再生治療法創出
    科学研究費助成事業
    2025年04月01日 - 2028年03月31日
    西田 絵利香; 宮治 裕史; 蔀 佳奈子; 黒嶋 伸一郎; 大矢根 綾子; 島袋 将弥
    日本学術振興会, 基盤研究(C), 北海道大学, 25K13061
  • インプラント周囲炎治療のための抗菌・骨形成促進ナノ粒子の術中3次元デリバリー
    科学研究費助成事業
    2024年04月01日 - 2028年03月31日
    中村 真紀; 奈良崎 愛子; 宮治 裕史; 西田 絵利香
    日本学術振興会, 基盤研究(B), 国立研究開発法人産業技術総合研究所, 24K03292
  • レーザーによる抗菌加工面が健康長寿に貢献！新たな口腔カンジダ症治療への展開
    科学研究費助成事業
    2025年04月 - 2028年03月
    田中 佐織; 宮治裕史; 大矢根綾子; 西田絵利香
    日本学術振興会, 基盤研究(C), 北海道大学, 25K12999
  • インプラント周囲炎治療のための抗菌・骨形成促進ナノ粒子の術中3次元デリバリー
    科学研究費助成事業
    2024年04月 - 2028年03月
    中村 真紀; 奈良崎 愛子; 宮治 裕史; 西田 絵利香
    日本学術振興会, 基盤研究(B), 国立研究開発法人産業技術総合研究所, 24K03292
  • 人工バイオミネラリゼーションの光制御と歯面機能デザイン
    科学研究費助成事業
    2024年04月01日 - 2027年03月31日
    大矢根 綾子; 宮治 裕史; 西田 絵利香
    日本学術振興会, 基盤研究(B), 国立研究開発法人産業技術総合研究所, 24K03294
  • ２段階薬剤放出性第４世代スキャフォールドの歯周組織再生への応用
    科学研究費助成事業
    2022年04月 - 2025年03月
    西田 絵利香; 宮治 裕史; 大矢根 綾子; 中村 真紀; 赤坂 司
    アパタイト2層構造化による２段階薬剤放出性第４世代スキャフォールド構築のため，初年度はまず1層目の構築・評価を行った．
    1層目として，アパタイトの難溶化（溶解性低下）と抗菌性の期待される生体微量元素（フッ素）を種々の濃度（0～1000 uM）でCaP過飽和溶液に添加し，スキャフォールド上にフッ素含有アパタイト層を作製した．
    その結果，スキャフォールド内部および外部表面への均一なフッ素含有アパタイト層の生成を確認し，過飽和溶液へのフッ素添加濃度の増加により生成するアパタイト層のフッ素置換率（F/P比で評価）向上が認められた．
    成膜後のスキャフォールドを生食に浸漬して溶解性等を評価したところ，過飽和溶液へのフッ素添加濃度の増加に伴い，生食中へのアパタイトの溶解量（7日後）は減少した．アパタイト結晶にフッ化物イオンが取り込まれることにより，層の難溶化したと考えられる，また，生食中へのフッ素溶出量も増加した．
    以上より，フッ化物イオンをバイオミメティックコーティング法によりアパタイト層に取り込むことに成功した．フッ化物濃度の増加とともにアパタイト層の溶解度が低下し，減少した．
    日本学術振興会, 基盤研究(C), 北海道大学, 22K10012
  • 高周波電流によるファイル未到達根管における歯髄の蒸散と根管の殺菌
    科学研究費助成事業
    2021年04月 - 2024年03月
    菅谷 勉; 西田 絵利香
    牛象牙質にΦ0.1㎜、0.4mmの模擬根管を１つまたは２つ作製し、電圧225Vで通電を行って、各根管壁の焼灼状態をSEMおよびEDSで評価した。その結果、通電時間を長くすると根管壁からCやOの検出が低下し、さらに通電時間をながくするとCaとPのみが検出されるようになって、SEMでは溶岩状に象牙質が溶融凝固した部位が増加した。この焼灼効果はΦ0.1㎜よりΦ0.4mmの方が高く、4秒の通電で根管壁はほぼ全面が溶融凝固した像を示した。Φ0.1mmと0.4mmの2根管モデルでは、それぞれ単根管モデルとほぼ同様の変化を示し、通電時間が2秒ではΦ0.1mmの根管壁に有機質が多量に残存したが、４秒に延長すると80％が蒸散可能であった。さらに、根管壁に炭化物の残存はなく、蒸散と洗浄が同時に行われている可能性が示された。
    一方、イヌの健全歯を髄腔開拡し、高周波電流を通電して歯髄の焼灼状態を病理組織学的に検討した。その結果、ファイルが根尖孔から1-2mm歯冠側で150または225Vを1秒印加することで、主根管および根尖分枝内の歯髄は壊死し、とくに血管は構造を失って研究や血漿の変性が認められた。根尖から4mm歯冠側の位置で225Vを1秒通電しても、概ね同様の結果であったが、根尖分枝内ではわずかに残髄する可能性が見られた。また、歯根膜には225Vを印加した場合にはわずかに蒸散がみられたが、その範囲は10μm程度であり、その周囲に熱変性は見られなかった。しかし、歯根膜にファイルが接した状態で通電すると、歯槽骨吸収も観察された。さらに根尖を穿通してグライドパス後に通電を行うと、歯根膜への機械的損傷による炎症が生じるとともに、根尖分枝内の焼灼がやや不十分になった。
    日本学術振興会, 基盤研究(C), 北海道大学, 21K09908
  • 根面う蝕の撲滅を目指して！バイオミメティック法を用いたセメント質強化法の確立
    科学研究費助成事業
    2019年04月01日 - 2023年03月31日
    田中 佐織; 大矢根 綾子; 宮治 裕史; 西田 絵利香; 田中 享
    レーザー援用バイオミメティック法により，露出歯根セメント質上に抗菌性アパタイトをコーティングし，新しい根面う蝕予防法及び進行抑制法を開発することを目的とした.
    1.NaFを添加したCaP過飽和溶液中のセメント質基材にレーザ照射後，表面分析し照射面にFが検出された．Ca/C元素比の上昇から，照射面にフッ素含有アパタイトの生成を確認した． 2.NaF添加CaP過飽和溶液中のセメント質に半導体レーザーを照射した。照射後の表面にFApが成膜され，未照射表面と比較し，ヌープ硬度の有意な増加が認められた．3.脱灰抑制効果が確認された.4.ビーグル犬を用いた実験より口腔内への応用の可能性を見出した．
    日本学術振興会, 基盤研究(C), 北海道大学, 19K10103
  • 根面う蝕の撲滅を目指して！バイオミメティック法を用いたセメント質強化法の確立
    科学研究費助成事業
    2019年04月 - 2023年03月
    田中 佐織; 大矢根 綾子; 宮治 裕史; 西田 絵利香; 田中 享
    本研究は，レーザー援用バイオミメティック法により，露出歯根セメント質上にアパタイトをコーティングして，新しい根面う蝕予防法及び進行抑制法を開発，実現することを目的として行われる.(1)セメント質面にアパタイトを析出させることができるか(2)生理活性物質を添加し，セメント質の無機質（アパタイト）及び有機質（コラーゲン）を保護し耐酸性を向上させることができるかを明らかにすることである．
    R1年度はNaFを添加したCaP過飽和溶液中にセメント質基材（直径約5 mm）にNd:YAGナノ秒パルスレーザーの非集光ビーム（355 nm, 6 W/cm2）を10分間照射後，照射面および非照射面を，走査電子顕微鏡（SEM）とエネルギー分散型X線分析装置（EDX）で分析した．EDX分析の結果，照射面にはCaとPに加え新たにFが検出され，Ca/C元素比の上昇が認められた．これらの結果は，照射面にフッ素含有アパタイト（FAp）が生成したことを示した．
    R2～3年度は飽和液中レーザー照射法により、セメント質基材を作製した。基材の表面に、光吸収剤を塗布し、乾燥させた。フッ化ナトリウム（1 mM）を添加したリン酸カルシウム過飽和溶液中に基材を設置し、その表面にレーザー光（昭和薬品化工株式会社製Sレーザー、波長：808 nm）を3分間照射した。照射距離は10 mm、出力は3 Wとした．超純水にて洗浄，風乾し，未照射および照射後の表面のヌープ硬度(KHN)を測定した.照射後のセメント質表面にFApが成膜されたことで，未照射セメント質表面と比較して，KHNの有意な増加が認められた．わずか3分の過飽和液中レーザー照射によってFApを成膜できることを確認した．耐酸性をKHNで測定した結果，測定値にばらつきがみられたため，追加実験の実施のために歯基材を調整している．
    日本学術振興会, 基盤研究(C), 北海道大学, 19K10103
  • 世界初！ナノセラミクス配合ヒトコラーゲンによる安心安全な歯周組織再生法
    科学研究費助成事業
    2018年04月 - 2022年03月
    西田 絵利香
    ヒトⅠ型コラーゲン様リコンビナントペプチドは，動物由来成分を含まないバイオマテリアルとして注目されている．これを顆粒状に成形し（RCP），さらにβ型リン酸三カルシウム（β-TCP）微粒子を配合した作製したβ-TCP/RCPは，in vitro評価及びラット頭蓋骨欠損モデルによる評価によって，良好な生体親和性と骨形成能を示した．次にビーグル犬で歯周組織再生効果があるかどうかを検討した．術後8週後，実験群の新生骨量は対照群より2.2倍多かった．また，歯根膜様組織も2.1倍多く形成され，歯肉退縮を有意に抑制した．以上よりβ-TCP/RCPは歯周組織再生材料として有効であることが示唆された．
    日本学術振興会, 若手研究, 北海道大学, 18K17038
  • バイオミネラリゼーション法による歯周組織再生用ナノ材料の創製と応用
    科学研究費助成事業
    2016年04月 - 2019年03月
    宮治 裕史; 田中 佐織; 加藤 昭人; 中塚 愛; 西田 絵利香; 大矢根 綾子
    生体内での骨形成を模したバイオミネラリゼーション法を用いて，ナノ構造化アパタイトスキャフォールドを創製し，生体親和性，骨増生効果および歯周組織再生効果を検討した．またアパタイトへ線維芽細胞増殖因子2（FGF2）を複合化し，スキャフォールドの高機能化を図った．
    その結果，アパタイトスキャフォールドの生体親和性は良好で，ラット頭蓋骨の増生を有意に促進し，ビーグル犬の2級分岐部骨欠損において歯周組織再生効果を示した．また，アパタイトスキャフォールドへのFGF2の複合化は骨形成を強く促進した．以上よりナノ構造化アパタイトスキャフォールドは有用な骨再生材料であることが示された．
    日本学術振興会, 基盤研究(C), 北海道大学, 16K11822
  • 生体内でレジン上に歯周組織形成を実現！～接着治療法の予知性向上を目指して～
    科学研究費助成事業
    2016年04月 - 2019年03月
    田中 佐織; 宮治 裕史; 田中 享; 西田 絵利香; 大矢根 綾子; 中村 真紀; 加藤 昭人; 宮田 さほり; 眞弓 佳代子
    レジン表面に歯周組織形成を目指し、レーザー援用バイオミメティック法(LAB法)を用いたレジン表面の改変を検討した.LAB法を応用すると，レジン表面にマイクロスケールの板状結晶よりなるCaP析出物の形成が認められた.その後擬似体液に浸漬すると，析出したCaP上に緻密なアパタイト層が形成された．レジン表面のCaPと生理活性物質であるフィブロネクチン（Fn)を複合化処理すると，CaP-Fn処理した試料は，Cap処理のみ，及び処理なしの試料と比較して，明らかに多数の細胞が付着していた.以上より，レジン表面へLAB法を応用すれば，表面の生体適合性を改良し，新しい歯周組織再生の方法となる可能性が示された.
    日本学術振興会, 基盤研究(C), 北海道大学, 16K11538
  • Ag/ナノカーボンを用いた抗菌性スキャフォールドの開発と歯周組織再生効果
    科学研究費助成事業
    2016年08月 - 2018年03月
    西田 絵利香
    本研究ではAg担持型ナノカーボンスキャフォールドを創製し，歯周組織再生効果を実証することを目的とした．GO，Ag/GOスキャフォールドに細胞為害性は認められず，細菌培養試験ではAg/GOスキャフォールドで細菌増殖抑制効果を認めた．また，ラットでの組織学的観察ではコラーゲンスキャフォールドに比較して，GO,Ag/GOスキャフォールドは生体親和性は良好であった．イヌの抜歯窩埋入実験では，GO,Ag/GOスキャフォールドで骨再生に大きな差を認めなかった．
    以上よりGOコーティングはスキャフォールドの特性を向上させ，Ag/GOスキャフォールドは抗菌性を発揮できる可能性が示唆された．
    日本学術振興会, 研究活動スタート支援, 北海道大学, 16H06604
  • 多孔質体を形成するエマルション骨ペーストの歯周病治療への応用
    科学研究費助成事業
    2014年04月 - 2017年03月
    加藤 昭人; 西田 絵利香; 舘山 彰人
    組織再生の細胞が内部に入りやすい空隙を多数形成するα-リン酸三カルシウム（α-TCP）とポリ乳酸グリコール酸共重合体（PLGA）微粒子から成るエマルション骨ペーストを開発し，骨再生材料としての能力を生体内外で評価した．その結果，エマルション骨ペーストは生体内での硬化で良好な生体親和性を示し，PLGA添加でより多くの細胞の材料内への侵入を認めた．この新しい骨ペーストを歯周病で失われた骨に応用できれば，硬化後に周りから大量の細胞が入り込んで，従来の骨移植材以上の再生効果が得られると期待される．
    日本学術振興会, 若手研究(B), 北海道大学, 26870016
  • ナノカーボンコーティングを用いた新規歯周病治療法
    科学研究費助成事業
    2013年04月 - 2016年03月
    宮治 裕史; 田中 佐織; 加藤 昭人; 古月 文志; 西田 絵利香
    ナノカーボン素材の生物学的特性を評価するために，酸化グラフェン（GO）ならびにカーボンナノチューブ（CNT）の生体親和性を検討した．またGOを用いた歯の抗菌コーティング法，ならびにGOスキャフォールドによる再生療法を開発して歯周病治療法としての有効性を検討した．その結果，GO，CNTともに低濃度において生体親和性は良好で，CNTは細胞増殖促進効果を発揮した．一方GOによる歯のコーティング処理によって抗菌性歯面を獲得することができた．また，GOスキャフォールドは細胞親和性が高く，抜歯窩や根分岐部骨欠損に埋入すると骨新生を強く促進した．以上よりナノカーボン材料は有用な医用材料であることが示された．
    日本学術振興会, 基盤研究(C), 北海道大学, 25463210
  • ナノ技術を応用したレジン上への歯周組織再生を伴う接着治療法の開発
    科学研究費助成事業
    2013年04月 - 2016年03月
    田中 佐織; 宮治 裕史; 井上 加菜; 田中 享; 谷野 美智恵; 加藤 昭人; 金山 和泉; 西田 絵利香; 村上 秀輔; 川本 康平; 宮田 さほり
    歯根破折歯接着治療法の予知性向上を目的として，封鎖材料のレジン表面を改変し培養細胞シートを貼ることでレジン上に歯周組織再生を目指した.レジン表面をカーボンナノチューブ（CNT）とナノβ-TCPでコーティングした.細胞付着試験では，CNTコートした試料に付着した細胞はコントロールと比較すると多く，細胞伸展が良好であった.また細胞増殖性試験でも高い細胞増殖性がみられた。皮下埋植試験では著明な炎症反応は認められず，CNTコートした試料に培養細胞シートを貼った試料周囲に骨様組織形成が認められた.以上よりレジン表面を改変することにによりレジン上に歯周組織再生の可能性が見出された.
    日本学術振興会, 基盤研究(C), 北海道大学, 25462943
■ 産業財産権
  • 亜鉛含有抗菌剤
    特許権, 宮治 裕史; 西田 絵利香; 蔀 佳奈子; 浜本 朝子; 小野 博信; 郷田 隼, 国立大学法人北海道大学, 株式会社日本触媒
    特願2018-207370, 2018年11月02日
    特開2020-070277, 2020年05月07日
    特許第7190132号, 2022年12月07日
    202203006817539960
  • 抗菌剤組成物
    特許権, 宮治 裕史; 西田 絵利香; 蔀 佳奈子; 小野 博信; 郷田 隼, 国立大学法人北海道大学, 株式会社日本触媒
    特願2018-207371, 2018年11月02日
    特開2020-070278, 2020年05月07日
    特許第7177436号, 2022年11月15日
    202203006940233921
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