研究者基本情報

• 博士(理学), 総合研究大学院大学

• https://researchmap.jp/read0125123

• https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200901085668269498
• https://anatomy3.hokkaido.university/

• 200901085668269498

• 大脳皮質
• 神経生理学
• 大脳基底核
• 聴覚

• ライフサイエンス, 神経科学一般

研究活動情報

• Globus pallidus is not independent from striatal direct pathway neurons: an up-to-date review
  Fumino Fujiyama; Fuyuki Karube; Yasuharu Hirai
  Mol Brain, 17, 34, 2024年, ［査読有り］
  英語, 研究論文（学術雑誌）
• High resolution recording of local field currents simultaneously with sound-evoked calcium signals by a photometric patch electrode in the auditory cortex field L of the chick.
  Harunori Ohmori; Yasuharu Hirai; Ryosuke Matsui; Dai Watanabe
  Journal of neuroscience methods, 392, 109863, 109863, 2023年05月15日, ［国際誌］
  英語, 研究論文（学術雑誌）, BACKGROUND: Functioning of the brain is based on both electrical and metabolic activity of neural ensembles. Accordingly, it would be useful to measure intracellular metabolic signaling simultaneously with electrical activity in the brain in vivo. NEW METHOD: We innovated a PhotoMetric-patch-Electrode (PME) recording system that has a high temporal resolution incorporating a photomultiplier tube as a light detector. The PME is fabricated from a quartz glass capillary to transmit light as a light guide, and it can detect electrical signals as a patch electrode simultaneously with a fluorescence signal. RESULTS: We measured the sound-evoked Local Field Current (LFC) and fluorescence Ca2+ signal from neurons labeled with Ca2+-sensitive dye Oregon Green BAPTA1 in field L, the avian auditory cortex. Sound stimulation evoked multi-unit spike bursts and Ca2+ signals, and enhanced the fluctuation of LFC. After a brief sound stimulation, the cross-correlation between LFC and Ca2+ signal was prolonged. D-AP5 (antagonist for NMDA receptors) suppressed the sound-evoked Ca2+ signal when applied locally by pressure from the tip of PME. COMPARISON WITH EXISTING METHODS: In contrast to existing multiphoton imaging or optical fiber recording methods, the PME is a patch electrode pulled simply from a quartz glass capillary and can measure fluorescence signals at the tip simultaneously with electrical signal at any depth of the brain structure. CONCLUSION: The PME is devised to record electrical and optical signals simultaneously with high temporal resolution. Moreover, it can inject chemical agents dissolved in the tip-filling medium locally by pressure, allowing manipulation of neural activity pharmacologically.
• Corticocortical innervation subtypes of layer 5 intratelencephalic cells in the murine secondary motor cortex.
  Sanghun Im; Yoshifumi Ueta; Takeshi Otsuka; Mieko Morishima; Mohammed Youssef; Yasuharu Hirai; Kenta Kobayashi; Ryosuke Kaneko; Kenji Morita; Yasuo Kawaguchi
  Cerebral Cortex, 33, 1, 50, 67, 2022年12月15日, ［査読有り］, ［国際誌］
  英語, 研究論文（学術雑誌）, Feedback projections from the secondary motor cortex (M2) to the primary motor and sensory cortices are essential for behavior selection and sensory perception. Intratelencephalic (IT) cells in layer 5 (L5) contribute feedback projections to diverse cortical areas. Here we show that L5 IT cells participating in feedback connections to layer 1 (L1) exhibit distinct projection patterns, genetic profiles, and electrophysiological properties relative to other L5 IT cells. An analysis of the MouseLight database found that L5 IT cells preferentially targeting L1 project broadly to more cortical regions, including the perirhinal and auditory cortices, and innervate a larger volume of striatum than the other L5 IT cells. We found experimentally that in upper L5 (L5a), ER81 (ETV1) was found more often in L1-preferring IT cells, and in IT cells projecting to perirhinal/auditory regions than those projecting to primary motor or somatosensory regions. The perirhinal region-projecting L5a IT cells were synaptically connected to each other and displayed lower input resistance than contra-M2 projecting IT cells including L1-preferring and nonpreferring cells. Our findings suggest that M2-L5a IT L1-preferring cells exhibit stronger ER81 expression and broader cortical/striatal projection fields than do cells that do not preferentially target L1.
• Conservation of the Direct and Indirect Pathway Dichotomy in Mouse Caudal Striatum With Uneven Distribution of Dopamine Receptor D1- and D2-Expressing Neurons
  Kumiko Ogata; Fuko Kadono; Yasuharu Hirai; Ken-ichi Inoue; Masahiko Takada; Fuyuki Karube; Fumino Fujiyama
  Frontiers in Neuroanatomy, 2022年02月, ［査読有り］
  英語, 研究論文（学術雑誌）
• Ipsi- and contralateral corticocortical projection-dependent subcircuits in layer 2 of the rat frontal cortex
  Yoshifumi Ueta; Jaerin Sohn; Fransiscus Adrian Agahari; Sanghun Im; Yasuharu Hirai; Mariko Miyata; and Yasuo Kawaguchi
  Journal of Neurophysiology, 122, 4, 1461, 1472, 2019年10月, ［査読有り］
• Parvalbumin-producing striatal interneurons receive excitatory inputs onto proximal dendrites from the motor thalamus in male mice.
  Nakano Y; Karube F; Hirai Y; Kobayashi K; Hioki H; Okamoto S; Kameda H; Fujiyama F
  Journal of Neuroscience Research, 96, 7, 1186, 1207, 2018年07月, ［査読有り］
• Simultaneous recording of fluorescence and electrical signals by photometric patch electrode in deep brain regions in vivo.
  Hirai Y; Nishino E; Ohmori H
  Journal of neurophysiology, 113, 10, 3930, 3942, 2015年06月, ［査読有り］
• Direction- and distance-dependent interareal connectivity of pyramidal cell subpopulations in the rat frontal cortex.
  Ueta Y; Hirai Y; Otsuka T; Kawaguchi Y
  Frontiers in neural circuits, 7, OCT, 164, 2013年, ［査読有り］
  英語
• Specialized cortical subnetworks differentially connect frontal cortex to parahippocampal areas.
  Hirai Y; Morishima M; Karube F; Kawaguchi Y
  The Journal of neuroscience : the official journal of the Society for Neuroscience, 32, 5, 1898, 1913, 2012年02月, ［査読有り］
  英語
• Selective coexpression of multiple chemical markers defines discrete populations of neocortical GABAergic neurons.
  Kubota Y; Shigematsu N; Karube F; Sekigawa A; Kato S; Yamaguchi N; Hirai Y; Morishima M; Kawaguchi Y
  Cerebral cortex (New York, N.Y. : 1991), 21, 8, 1803, 1817, 2011年08月, ［査読有り］
  英語
• Quantitative chemical composition of cortical GABAergic neurons revealed in transgenic venus-expressing rats.
  Uematsu M; Hirai Y; Karube F; Ebihara S; Kato M; Abe K; Obata K; Yoshida S; Hirabayashi M; Yanagawa Y; Kawaguchi Y
  Cerebral cortex (New York, N.Y. : 1991), 18, 2, 315, 330, 2008年02月, ［査読有り］
  英語

• Local and distal connections of neocortical pyramidal cells projecting to the perirhinal regions
  Yasuharu Hirai; Mieko Morishima; Yasuo Kawaguchi, NEUROSCIENCE RESEARCH, 68, E391, E392, 2010年
  英語, 研究発表ペーパー・要旨（国際会議）
• Pyramidal neuron subtypes of rat frontal cortex projecting to the perirhinal cortex
  Yasuharu Hirai; Mieko Morishima; Yasuo Kawaguchi, NEUROSCIENCE RESEARCH, 61, S76, S76, 2008年
  英語, 研究発表ペーパー・要旨（国際会議）

• 日本生理学会
• 日本神経科学学会