food chemistry

LC/MS

lipidomics

analytical chemistry

Life Science, Nutrition science and health science

Life Science, Food sciences

Others, Others, 分析化学

Mar. 2026, 令和7年度北海道大学大学院保健科学院 保健科学院長賞

Nov. 2025, 令和6年度北海道大学大学院保健科学院 研究奨励賞

Oct. 2025, The 7th FHS International Conference Best Poster Award

Aug. 2024, 第64回日本臨床化学会年次学術集会 JSCC Student Award

Sep. 2023, 第48回日本医用マススペクトル学会年会 若手優秀ポスター賞

Mar. 2023, 北海道大学大学院保健科学院 修士課程研究発表賞（Best Presentation Award）

Oct. 2022, 第8回北海道大学部局横断シンポジウム ベストポスター賞

Hydrogen gas with hypothermic machine perfusion induces lipidomic alterations in donation-after-cardiac-death rat livers
Yusuke Minami; Siddabasave Gowda B. Gowda; Kengo Shibata; Sodai Sakamoto; Divyavani Gowda; Tsuyoshi Shimamura; Akinobu Taketomi; Hitoshi Chiba; Moto Fukai; Shu-Ping Hui
Journal of Lipid Research, 101040, 101040, Elsevier BV, Apr. 2026, [Peer-reviewed], [Lead author]
Scientific journal

Sex- and Regio-Specific Lipid Profiling of Shishamo and Capelin Fish by Nontargeted Liquid Chromatography/Mass Spectrometry
Yusuke Minami; Siddabasave Gowda B. Gowda; Divyavani Gowda; Hitoshi Chiba; Shu-Ping Hui
Foods, 15, 2, 298, 298, MDPI AG, 14 Jan. 2026, [Peer-reviewed], [Lead author]
Scientific journal, Shishamo smelt (Spirinchus lanceolatus), which is endemic to Hokkaido, Japan, is frequently substituted in markets with morphologically similar capelin (Mallotus villosus) imported from abroad. Lipids are essential nutrients that play important roles in fish authenticity, validation, and nutritional assessment. Although shishamo has long been consumed in Japan, its region-specific lipid distribution and composition are different from those of capelin and have not been well explored. To overcome these gaps, we used untargeted liquid chromatography–mass spectrometry to profile sex- and region-specific lipids in the Japanese S. lanceolatus and Nordic M. villosus. The results revealed that female S. lanceolatus heads exhibited high triacylglycerol (TAG) content, closely resembling roe composition. Docosahexaenoic acid and eicosapentaenoic acid were enriched in the female S. lanceolatus viscera. Multivariate analysis identified monounsaturated fatty acids, such as fatty acid (FA) 22:1 and FA 20:1, as robust discriminatory markers between S. lanceolatus and M. villosus. Overall, sex- and regiospecific differences in lipid composition between the two species were correlated and compared. These lipidomic signatures provide a basis for verifying species authenticity and geographic origin, while highlighting the nutritional lipid potential of S. lanceolatus, particularly from the female viscera.

Regio-specific lipid fingerprinting of edible sea cucumbers using LC/MS
Yusuke Minami; Siddabasave Gowda B. Gowda; Divyavani Gowda; Hitoshi Chiba; Shu-Ping Hui
Food Research International, 184, 114253, 114253, Elsevier BV, May 2024, [Peer-reviewed], [Lead author]
Scientific journal

A facile method for monitoring sphingomyelin synthase activity in HeLa cells using liquid chromatography/mass spectrometry
Punith M. Sundaraswamy; Yusuke Minami; Jayashankar Jayaprakash; Siddabasave Gowda B. Gowda; Hiroyuki Takatsu; Divyavani Gowda; Hye-Won Shin; Shu-Ping Hui
Analyst, 149, 12, 3293, 3301, Royal Society of Chemistry (RSC), 2024, [Peer-reviewed], [Lead author]
Scientific journal, An LC-MS/MS-based assay for monitoring sphingomyelin synthase (SMS) activity was developed. The method is highly useful in screening novel inhibitors for SMS.

Detection and characterization of lipids in eleven species of fish by non-targeted liquid chromatography/mass spectrometry
Siddabasave Gowda B. Gowda; Yusuke Minami; Divyavani Gowda; Hitoshi Chiba; Shu-Ping Hui
Food Chemistry, 393, 133402, 133402, Elsevier BV, Nov. 2022, [Peer-reviewed], [Lead author]
Scientific journal

Docosahexaenoic Acid Esters of Hydroxy Fatty Acid Is a Novel Activator of NRF2
Siddabasave Gowda B. Gowda; Takayuki Tsukui; Hirotoshi Fuda; Yusuke Minami; Divyavani Gowda; Hitoshi Chiba; Shu-Ping Hui
International Journal of Molecular Sciences, 22, 14, 7598, 7598, MDPI AG, 15 Jul. 2021, [Peer-reviewed]
Scientific journal, Fatty acid esters of hydroxy fatty acids (FAHFAs) are a new class of endogenous lipids with interesting physiological functions in mammals. Despite their structural diversity and links with nuclear factor erythroid 2-related factor 2 (NRF2) biosynthesis, FAHFAs are less explored as NRF2 activators. Herein, we examined for the first time the synthetic docosahexaenoic acid esters of 12-hydroxy stearic acid (12-DHAHSA) or oleic acid (12-DHAHOA) against NRF2 activation in cultured human hepatoma-derived cells (C3A). The effect of DHA-derived FAHFAs on lipid metabolism was explored by the nontargeted lipidomic analysis using liquid chromatography-mass spectrometry. Furthermore, their action on lipid droplet (LD) oxidation was investigated by the fluorescence imaging technique. The DHA-derived FAHFAs showed less cytotoxicity compared to their native fatty acids and activated the NRF2 in a dose-dependent pattern. Treatment of 12-DHAHOA with C3A cells upregulated the cellular triacylglycerol levels by 17-fold compared to the untreated group. Fluorescence imaging analysis also revealed the suppression of the degree of LDs oxidation upon treatment with 12-DHAHSA. Overall, these results suggest that DHA-derived FAHFAs as novel and potent activators of NRF2 with plausible antioxidant function.

Lipidomic analysis of non-esterified furan fatty acids and fatty acid compositions in dietary shellfish and salmon by UHPLC/LTQ-Orbitrap-MS
Siddabasave Gowda B. Gowda; Yusuke Minami; Divyavani Gowda; Daisuke Furuko; Hitoshi Chiba; Shu-Ping Hui
Food Research International, 144, 110325, 110325, Elsevier BV, Jun. 2021, [Peer-reviewed]
Scientific journal

脳組織リピドームと動物生息環境の相関
Siddabasave Gowda B. GOWDA; 南雄介; 惠淑萍, 臨床化学, 55, 2, 110, 114, 2026, [Invited]

Lipidomics for myocardial infarction biomarker discovery
南 雄介; ボメゴウダ シッダバサーブゴウダ; 千葉仁志; 惠淑萍, JSBMS Letters, 48, 2, 20, 26, Jun. 2023, [Invited], [Lead author]

Difference in health functional lipids between Shishamo and Capelin (Karafuto-Shishamo)
Yusuke Minami; Siddabasave Gowda; B. Gowda; Divyavani Gowda; Shu-Ping Hui
7th FHS International Conference (FHS2025), Oct. 2025

低温機械灌流と水素ガス併用治療が心停止ラット肝の脂質代謝に与える影響
南 雄介; ボメゴウダ シッダバサーブゴウダ; 深井 原; 坂本 聡大; 柴田 賢吾; 嶋村 剛; 武冨 紹信; 惠 淑萍
第35回日本臨床化学会北海道支部例会, Sep. 2025

Differences in sex- and body part-specific lipid profiles of shishamo and capelin (Karafuto-shishamo)
Yusuke Minami; Siddabasave Gowda; B. Gowda; Divyavani Gowda; Shu-Ping Hui
International Symposium 2025, Sustainability in Agriculture, Food and Health, Hokkaido University, Sep. 2025

シシャモおよびカラフトシシャモの健康機能性脂質の違い
南 雄介; Bomme Gowda SIDDABASAVE GOWDA; Divyavani GOWDA; 千葉 仁志; 惠 淑萍
第79回日本栄養・食糧学会大会, May 2025

肝虚血再灌流障害モデルラットの脂質代謝に及ぼす水素ガスの影響
南 雄介; ボメゴウダ; シッダバサーブゴウダ; 深井 原; 坂本 聡大; 柴田 賢吾; 嶋村剛; 武冨 紹信; 千葉 仁志; 惠 淑萍
第64回日本臨床化学会年次学術集会, Aug. 2024

非標的液体クロマトグラフィー/質量分析による食用ナマコの機能性脂質分析
南 雄介; ボメゴウダ シッダバサーブゴウダ; デヴィヤヴァニ ゴウダ; 千葉 仁志; 惠 淑萍
第63回日本臨床化学会年次学術集会, Oct. 2023

Functional lipids analysis of sea cucumber using non-targeted LC/MS method.
Yusuke Minami; Siddabasave Gowda; B. Gowda; Divyavani Gowda; Hitoshi chiba; Shu-Ping Hui
6th FHS International Conference (FHS2023), Oct. 2023

非標的LC/MSを用いたナマコ中の健康機能性脂質の分析と同定
南 雄介; ボメゴウダ シッダバサーブゴウダ; デヴィヤヴァニ ゴウダ; 千葉 仁志
第48回日本医用マススペクトル学会年会, Sep. 2023

Functional lipid analysis of edible sea cucumber by non-targeted liquid chromatography/mass spectrometry
Minami, Y.; Gowda; S. G. B.; Chiba, H.; Hui, S. P.
The 31st Annual Meeting of International Congress on Nutrition and Integrative Medicine, Jul. 2023

脂質代謝に関連した肥満抑制のための食品生理活性物質の探索
南 雄介; Bomme Gowda Siddabasave Gowda; Divyavani Gowda; 千葉 仁志; 惠 淑萍
第8回北海道大学部局横断シンポジウム, Oct. 2022

Discovery of food derived inhibitor for sphingolipid metabolism associated to obesity
南 雄介; Bomme Gowda Siddabasave Gowda; Divyavani Gowda; 千葉 仁志; 惠 淑萍
第47回日本医用マススペクトル学会年会, Sep. 2022

肥満に関連するスフィンゴ脂質代謝に対する食品由来阻害剤の発見
南 雄介; Bomme Gowda Siddabasave Gowda; Divyavani Gowda; 千葉 仁志; 惠 淑萍
第62回日本臨床化学会年次学術集会, Sep. 2022

Ability of Biomedical Laboratory Scientists to turn the negative effects of non-face-to-face education into profits
Yusuke Minami
59th Congress of Korean Association of Medical Technologists & International conference, Oct. 2021

UHPLC/LTQ Orbitrap MS を用いた日本産魚類における脂質組成の網羅的解析
南 雄介; ボメゴウダ シッダバサーブゴウダ; デヴィヤヴァニ ゴウダ; 千葉 仁志; 惠 淑萍
第46回日本医用マススペクトル学会年会, Sep. 2021

2021 - Present
日本臨床化学会

2021 - Present
日本医用マススペクトル学会

肥満に関連するスフィンゴ脂質代謝を制御するシシャモ由来化合物の探索と医薬展開
科学研究費助成事業
Apr. 2024 - Mar. 2026
南 雄介
日本学術振興会, 特別研究員奨励費, 北海道大学, Principal investigator, 24KJ0261

Investigating Role of Shishamo Extract on Obesity Associated with Sphingolipid Metabolism
アンビシャス博士人材フェローシップ
Apr. 2023 - Mar. 2024
文部科学省「科学技術イノベーション創出に向けた大学フェローシップ創設事業」, Principal investigator