SEARCH

Search Details

Nakatomi Akiko

Faculty of ScienceProfessor
Institute for Academic InnovationProfessor

Researcher basic information

■ Degree
  • PhD, Hokkaido University, Mar. 2003
■ URL
researchmap URLホームページURL■ Various IDs
J-Global ID■ Research Keywords and Fields
Research Keyword
  • 細胞情報伝達機構
  • 構造と機能の相関
  • signal transduction
  • structure-function relationship
Research Field
  • Life Science, Functional biochemistry
  • Life Science, Structural biochemistry
■ Educational Organization

Career

■ Career
Career
  • 2025 - Present
    Hokkaido University, Faculty of Science, Professor
  • 2019 - 2025
    Hokkaido University, Faculty of Science, Associate Professor
  • 2014 - 2019
    Hokkaido University, Faculty of Science, Specially Appointed Associate Professor
  • 2010 - 2014
    Hokkaido University, Faculty of Science, Assistant Professor
  • 2007 - 2010
    Hokkaido University, Faculty of Advanced Life Science, 助教
  • 2006 - 2007
    Hokkaido University, Faculty of Advanced Life Science, 助手
  • 2003 - 2006
    Graduate School of Science, Hokkaido University, Instructor
Educational Background
  • Apr. 1998 - Mar. 2003, Hokkaido University, Graduate School of Science, Division of Chemistry

Research activity information

■ Papers
■ Lectures, oral presentations, etc.
  • Ran結合能を持つカルシニューリン結合タンパク質の発見とカルシニューリンの機能制御
    中冨 晶子
    第８１回日本生化学会大会、第３１回日本分子生物学会年会合同大会, 11 Dec. 2008, Japanese, Nominated symposium
    [Invited], [Domestic Conference]
  • 「カルモジュリンの構造と機能」
    矢澤道生; 中冨晶子
    生理学研究所研究会「カルシウムシグナリング研究の新潮流」, 2004, Japanese, Public discourse
    [Invited], [Domestic Conference]
■ Syllabus
  • ALP特別科目, 2024年, 修士課程, 大学院共通科目
  • 化学特別講義, 2024年, 修士課程, 総合化学院
  • 化学特別講義, 2024年, 修士課程, 総合化学院
  • 大学院共通授業科目（一般科目）：自然科学・応用科学, 2024年, 修士課程, 大学院共通科目
  • 理学共通演習Ⅰ, 2024年, 学士課程, 理学部
  • 理学共通演習Ⅱ, 2024年, 学士課程, 理学部
■ Affiliated academic society
  • Japanese Association for Protein Phosphatase Research
  • The Japanese Biochemical Society
■ Research Themes
  • Molecular mechanism of localized activation of myosin II isoforms in cell
    Grants-in-Aid for Scientific Research
    2006 - 2007
    MASAYUKI Takahashi; MICHIO Yazawa; AKIKO Nakatomi; AKIHIKO Yamagishi
    Nonmuscle myosin II plays a role in a number of cell motility processes. The activities of myosin II are regulated by phosphorylation of its regulatory light chain (RLC). To function in the cell, nonmuscle myosin II molecules assemble into filaments through their C-terminal tails. In mammalian cells, there are three isoforms of nonmuscle myosin II (IIA IIB and IIC). They most likely assemble in an isoform-specific mode to form homo-filaments in cells. In order to clarify the molecular mechanism of the localized activation of each myosin II isoform in the cell, we performed the following investigations in this project.
    1. Identification of the essential regions for filament assembly:
    The charge-reversal mutants of the previously proposed three charge clusters (Ni, E.1743-E1753; P1, K1842-K1852; P2, K1874-R1884) of EGFP-MHC-JIB were expressed in the MRC-5 SV1 TG1 cells. The P1 and P2 mutants were diffused in the cell, indicating their importance for the filament assembly.
    2. Analyses of the filament mode of myosin II isoforms:
    Using FCCS analysis, we found that addition of Mtsl (S100A4), specifically stripped IIA rod fragments away from the pre-formed hetero-assemblies, and consequently, homo-assemblies of IIB rod fragments were formed.
    We demonstrated that the C-terminal 305-residue rod fragment of the myosin IIB heavy chain (BRF305) in SV1 cells induced unstable morphology like MHC-IIB^<-/-> fibroblasts. Furthermore, we proposed that the N-57 and C-63 of BRF305 are involved in self-recognition when myosin IIB molecules assemble into-homo-filamen
    3. Mechanism of the localized diphosphorylation of RLC in cell spreading:
    Immunofluorescence observation of the phosphorylated RLC and F-actin revealed that RLC of myosin IIA isoform was diphosphorylated at the transverse bundles in the lamella. Furthermore, it was indicated that Rho-kinase is involved in this diphosphorylation.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, 18570144
  • カルシウム/カルモジュリン依存性蛋白質脱リン酸化酵素カルシニューリンによる細胞内情報伝達機構の生化学的研究
    2002
    Competitive research funding
  • biochemistry of signal transduction via calcium/calmodulin dependent protein phosphatase, calcineurin
    2002
    Competitive research funding