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Yamagishi Masumi

Research Faculty of Agriculture　Fundamental AgriScience Research　Agrobiology and BioresourcesAssociate Professor

Researcher basic information

■ Degree
  • PhD (Agriculture), Kyoto University
■ URL
researchmap URL■ Various IDs
Researcher number
  • 40210348
J-Global ID■ Research Keywords and Fields
Research Keyword
  • 開花の早晩性
  • floral scent
  • transcriptional regulation
  • flower colour
  • Liliaceae
  • 園芸学
  • 育種学
  • ornamental crop
  • Horticultural Science
  • Plant breeding
Research Field
  • Environmental Science/Agriculture Science, Horticultural science
  • Life Science, Genetics
  • Environmental Science/Agriculture Science, Science in plant genetics and breeding
■ Educational Organization

Research activity information

■ Papers
■ Other Activities and Achievements
  • Effects of high temperature on anthocyanin accumulation in flowers and fruits
    Masumi Yamagishi, JAPR Journal, 59, 8, 12, 21, 2025, [Invited], [Lead author, Corresponding author]
    Japanese, Introduction commerce magazine
  • Floral Scent Emission in Lilies and Its Breeding
    Yamagishi, M., FFI Journal, 230, 1, 11, 15, 2025, [Invited], [Lead author, Corresponding author]
    Japanese, Introduction commerce magazine
  • ユリ野生種の遺伝資源としての利用
    山岸真澄, 農業および園芸, 93, 7, 576, 581, Jul. 2018, [Lead author, Corresponding author]
    Japanese
  • 利尻島に生育する栽培ヒナゲシ種子の札幌市における播種時期が発芽に及ぼす影響および生活史
    近藤哲也・吉田恵理・山岸真澄・愛甲哲也, 利尻研究, 31, 7, 17, 2012
    Japanese, Report research institution
  • スカシユリ花色の遺伝様式
    山岸 真澄, 農業および園芸, 80, 12, 1267, 1271, Dec. 2005
    養賢堂, Japanese
  • Shoot signals and molecular regulation of P-deficiency induced genes in cluster roots of white lupin
    Junqi Liu; Masumi Yamagishi; Claudia Uhde-Stone; Bruna Bucciarelli; Deborah Samac; Deborah Allan; Carroll Vance, Plant Nutrition for Food Security, Human Health and Environmental Protection. Proceedings of the 15th Plant Nutrition Colloquium, 94, 96, Sep. 2005
    English
  • Genetic analysis of floral pigmentation in Asiatic hybrid lily: mapping trait loci and candidate gene mapping approach
    Masumi Yamagishi; Akira Nakatsuka, Proceedings of 10th International Congress of SABRAO, A-08, The Society for the Advancement of Breeding Researches in Asia and Oceania, Aug. 2005, [Lead author]
    English
  • Differential activities and transcript levels of superoxide dismutases in two Solidago virgaurea populations from different altitudes
    MURAI, R; M. YAMAGISHI; S. TAKUMI; K. MURAI, Bulletin of Research Institute of Agricultural Resources, Ishikawa Agricultural College, 5, 5, 9, 16, 1997
    石川県農業短期大学農業資源研究所, English, Report research institution
  • 体細胞突然変異体の出現と変異形質の遺伝
    山岸真澄; 島田多喜子, 組織培養, 22, 11, 430, 435, Nov. 1996, [Lead author, Corresponding author]
    Japanese
  • Distorted segregation of RFLP markers in anther culture-derived rice plants
    YAMAGISHI, M; M. YANO; Y. FUKUTA; K. FUKUI; M. OTANI; T. SHIMADA, Proc. 2nd Asian Crop Sci. Conference, Fukui, Japan, 768, 769, Aug. 1996, [Lead author]
    English
  • Nodular callus induction and bulblet regeneration from anther tissue of Lilium longiflorum
    YAMAGISHI Masumi, Bulletin of Research Institute of Agricultural Resources, Ishikawa Agricultural College, 4, 52, 59, 1995
    English, Report research institution
  • Effects of mannose on enlargement of in vitro bulblets of Lilium japonicum Thunb
    YAMAGISHI Masumi, Bulletin of Research Institute of Agricultural Resources, Ishikawa Agricultural College, 4, 86, 89, 1995
    English, Report research institution
  • PCR法によるユリ属の種・品種の識別
    山岸真澄, 農耕と園芸, 1993年9月号, 50, 51, Sep. 1993, [Lead author]
    Japanese
  • Genetic analysis of rice mutants obtained by anther culture
    SHIMADA, T; M. YAMAGISHI; M. OTANI; T. KOBA; M. TAKAMURA; H. NIIZEKI, CURRENT PLANT SCIENCE AND BIOTECHNOLOGY IN AGRICULTURE, 15, 353, 356, 1993
    Introduction international proceedings
  • Effects of in vitro culture temperature and cold treatment on breakage of dormancy in bulblets of Lilium japonicum Thunb.
    YAMAGISHI Masumi, Bulletin of Research Institute of Agricultural Resources, Ishikawa Agricultural College, 3, 26, 30, 1993
    English, Report research institution
  • In vitro propagation of triploid Fritillaria camtschatcensis (L.) Ker-Gawler.
    YAMAGISHI Masumi, Bulletin of Research Institute of Agricultural Resources, Ishikawa Agricultural College, 2, 19, 26, 1991
    English, Report research institution
■ Books and other publications
  • Virus-induced gene silencing in lilies using Cucumber Mosaic Virus vectors. In: Courdavault V., Besseau S. (eds) Virus-Induced Gene Silencing in Plants: Methods and Protocols.
    Keisuke Tasaki; Masumi Yamagishi; Chikara Masuta, Methods in Molecular Biology, vol 2172. pp. 1–13
    Humana Press, 2020, [Contributor]
  • 4.3 Floriculture. In: Frontiers of Agricultural Science
    Masumi Yamagishi, pp. 98–101
    Research Faculty of Agriculture, Hokkaido University, 2015, [Contributor]
  • 鈴木正彦編、農学基礎シリーズ園芸学の基礎
    山岸 真澄, 5-II 品種の成り立ちと改良
    農文協, 2012, [Contributor]
  • イネの葯培養能力を支配する染色体領域（大川安信・岡成美編，第39回日本育種学会シンポジウム報告第２部・植物体再分化—ゲノムと遺伝子発現，育種学最近の進歩第39集）
    Masumi Yamagishi, pp. 29–32
    日本育種学会, 1997, [Contributor]
  • クロユリ（３倍体クロユリ）（農文協編，農業技術体系花卉編第５巻草花類の培養苗生産）
    Masumi Yamagishi, pp. 475–477
    農文協, 1997, [Contributor]
  • 三倍体クロユリの茎・葉からの子球増殖（新美芳二編，図解花のバイオ技術—増殖・育種とその関連技術）
    Masumi Yamagishi, p 52–53
    誠文堂新光社, 1992, [Contributor]
■ Syllabus
  • 農業植物科学特論, 2024年, 修士課程, 農学院
  • 農業植物科学特論演習, 2024年, 修士課程, 農学院
  • 園芸科学特論, 2024年, 修士課程, 農学院
  • 園芸科学特論演習, 2024年, 修士課程, 農学院
  • 一般教育演習(ﾌﾚｯｼｭﾏﾝｾﾐﾅｰ), 2024年, 学士課程, 全学教育
  • 生物資源科学特講, 2024年, 学士課程, 農学部
  • 生物資源科学実験Ⅰ, 2024年, 学士課程, 農学部
  • 細胞工学, 2024年, 学士課程, 農学部
■ Research Themes
  • ユリの花成誘導におけるageパスウェイの解明－種子繁殖性ユリの開発は可能か？
    科学研究費助成事業
    01 Apr. 2022 - 31 Mar. 2025
    山岸 真澄; 中塚 貴司
    ユリは幼若相の期間が長く、種子から育てると開花までに数年かかるため営利栽培では栄養繁殖されている。SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL) 遺伝子ファミリーは幼若相から成熟相への転換や花成誘導を制御する転写因子で、microRNA156 (miR156) によって負に制御され、花成を誘導するageパスウェイにおいて中心的な役割を担っている。これまでの研究よりユリのSPL遺伝子は花成ホルモンのFTよりもより密接に花成に関わっていると予測されている。本研究では、(1) ユリの形質転換体を用いたreverse geneticsにより個々のSPL遺伝子の機能を明らかにして、ユリの花成誘導機構の全体像を解明する。(2) miR156蓄積量の減少とSPLの発現を促進する内的・外的な要因を検討する。本研究の結果、多年生植物の多様な花成誘導機構がまた一つ明らかになる。相転換に関わるSPLの発現を促進する条件が分かれば、幼若相の期間を短縮する手がかりとなり、種子繁殖性ユリの開発につながる。
    これまでにSPL13a、SPL9、SPL15、MIM156(miR156の蓄積を抑制する)と、FT1、FT8を過剰発現させた形質転換ユリを育成し、遺伝子の機能解析を進めた。ユリのSPLは幼若相から成熟相への転換や花成誘導を制御するが、そのなかでも節間伸長（抽苔）に多大な効果を示すことが明らかになった。節間伸長に対するSPLの効果は他の植物種では報告されていない。得られた結果をもとにユリのageパスウェイ全容の解明を進める。
    日本学術振興会, 基盤研究(B), 北海道大学, 23K23596
  • ユリの花成誘導におけるageパスウェイの解明－種子繁殖性ユリの開発は可能か？
    科学研究費助成事業 基盤研究(B)
    Apr. 2022 - Mar. 2025
    山岸 真澄; 中塚 貴司
    日本学術振興会, 基盤研究(B), 北海道大学, Principal investigator, 22H02331
  • Roles of microRNA on flower color patterning in floricultural plants including lilies
    Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
    Apr. 2019 - Mar. 2022
    Masumi Yamagishi, Takashi Nakatsuka
    Some Asiatic hybrid lily cultivars develop bicolor tepals, which consist of anthocyanin-pigmented upper halves and un-pigmented lower halves. MYB12 positively regulating anthocyanin biosynthesis is downregulated in the lower halves. Since the lily MYB12 mRNA harbored a binding site for microRNA828 (miR828), the involvement of miR828 in variable spatial accumulation of MYB12 transcripts was evaluated. In the bicolor tepals, mature miR828 was more highly accumulated in the lower halves than in the upper halves, and miR828-directed cleavage of MYB12 transcripts was observed predominantly in the lower halves. Moreover, the cleavage triggered the production of secondary siRNA from MYB12 transcripts, and the siRNAs were accumulated predominantly in the lower halves. Consequently, miR828 suppressed MYB12 transcript accumulation in the white region, and the miR828/MYB12 module participated in the development of bicolor patterns in lily flowers.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, 19H02945
  • Study on microRNA involved in flower color pattern development in lilies
    秋山記念生命科学振興財団研究助成＜一般＞
    Apr. 2019 - Mar. 2020
    Masumi Yamagishi
    秋山記念生命科学振興財団, Principal investigator
  • オリエンタルハイブリッド起源種の多様性解析およびユリの栽培化に関わる遺伝子の探索
    基盤研究(C)
    Apr. 2015 - Mar. 2018
    半田高
    文部科学省, Competitive research funding
  • ユリのゲノム研究基盤を利用した花色・早晩性・香りの解析
    基盤研究(B)
    Apr. 2015 - Mar. 2018
    山岸 真澄
    文部科学省, Principal investigator, Competitive research funding
  • 転写型遺伝子サイレンシング（TGS）によるシンテッポウユリの花色改変
    挑戦的萌芽研究
    Apr. 2015 - Mar. 2017
    山岸 真澄
    文部科学省, Principal investigator, Competitive research funding
  • The establishment of the genome research bases and the evaluation of quantitative traits for flower colors in Lilium
    Grants-in-Aid for Scientific Research
    Apr. 2012 - Mar. 2015
    YAMAGISHI Masumi; MASUTA Chikara
    Lilies (Lilium spp.) are one of the most important floricultural crops worldwide. The lack of reverse genetics tools and gene-sequence information has made it challenging to evaluate the genetic backgrounds of useful traits such as flower colours. In this study, virus-induced gene silencing technique was established using Cucumber mosaic virus as a viral vector, and the sequences expressed in flower tepals were categorized using an RNA-seq technique. In addition, useful genes that determined flower colours in lilies were clarified: LhMYB12-Lat determined splatter-type flower spots in Asiatic hybrid lilies, LhMYB15 was responsible for the light-responsive bud-blush phenotype in Lilium regale, and two independent genes respectively generated white flowers in Lilium speciosum.
    Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B), Hokkaido University, Principal investigator, Competitive research funding, 24380014
  • Characterization of MYB genes regulating anthocyanin biosynthesis in Asiatic hybrid lily
    Grants-in-Aid for Scientific Research
    2010 - 2011
    YAMAGISHI Masumi
    A single dominant locus determines anthocyanin biosynthesis in the tepals of Asiatic hybrid lilies(Lilium spp.); however the gene that determines this trait has not been previously reported. Furthermore, anthocyanin colour hue in tepals varies among cultivars, but the mechanisms underlying this variation are not known. Here, we show that LhMYB12, which is homologous to petunia An2, determines anthocyanin pigmentation in tepals and that the level of LhMYB12 transcription affects the quantity of anthocyanin pigments, producing colour hue variation in tepals. The pink-tepal cultivars 'Montreux' and 'Renoir' were heterozygous for the LhMYB12 gene. Their F_1 population segregated 3 : 1 for the presence or absence of anthocyanin in tepals, and LhMYB12 cosegregated perfectly with the presence of anthocyanin, indicating that LhMYB12 determines anthocyanin biosynthesis in tepals. Among 7 cultivars, a single anthocyanin cyanidin 3-0-0-rutinoside was detected in tepals displaying light pink, pink-red, dark red, or chocolate brown hues. However, concentrations differed according to the following order of colour hue : light pinkJapan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C), Hokkaido University, Principal investigator, Competitive research funding, 22580022
  • Elucidation of the molecular mechanism involved with the formation of hermaphrodite and unisexual flowers in asparagus.
    Grants-in-Aid for Scientific Research(基盤研究(B))
    2008 - 2010
    Kiyoshi MASUDA; Masumi YAMAGISHI; Fuminori KOMAI; Masahiko SUZUKI
    Homozygous male of asparagus that is called super-male has been used as a pollinator for producing all-male hybrid seeds. However, we do not know a simplified method to discriminate homozygous males (YY) and heterozygous males (XY) in a short period. Then, we grew homozygous and heterozygous male plants from seeds produced by selfing of an andromonoecious line of asparagus and examined the method to identify their genotype. As a result, we found that these genotypes can be identified, based on quantitative analysis of a male-specific diagnostic marker, Asp1-T7.
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 20380014
  • 札幌キャンパスに自生するクロユリ集団の保全法の確立
    新渡戸基金研究助成
    2009
    山岸 真澄
    新渡戸基金研究助成, Principal investigator, Competitive research funding
  • Isolation of genes determining the flower color traits in Asiatic hybrid lily and their characterization by RNAi
    Grants-in-Aid for Scientific Research(基盤研究(B))
    2005 - 2007
    Masumi YAMAGISHI; 増田 清; 中務 明
    Because anthocyanin pigmentation in tepals of Asiatic hybrid lily is controlled by a single dominant gene and anthocyanin biosynthetic genes are expressed coordinately in tepals, some transcription factor is expected to control the presence or absence of anthocyanin pigment. In this study, two genes, LhbHLH1 and LhbHLH2 were isolated, which had homologies with PhJAF13 and PhAN1, respectively. LhbHLH1 and LhbHLH2 were expressed in tepals and leaves. When lily buds were exposed to light, anthocyanin pigment accumulated in leaves, which nearly disappeared 8 days after light exposure. Expressio...
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Principal investigator, Competitive research funding, 17380015
  • Molecular and biological analyses of a homeotic mutant and the regulation of floral organ formation in asparagus
    Grants-in-Aid for Scientific Research(基盤研究(B))
    2005 - 2007
    Kiyoshi MASUDA; 山岸 真澄; 星野 洋一郎
    The gender of asparagus is known to be determined by a male active factor M. Some lines of asparagus cultivars carry a homozygous genotype for M. These lines are practically called supermale, since all of the progeny express male phenotype, when pollens from these lines have been used for pollinaton. Therefore, the establishment of supermale lines of asparagus has been tried, in order to produce seed populations only with male genotype. To isolate the homozygous genotype on M male asparagus plants with andromonoecous phenotype, which are able to seed by self-fertilization, have been used. H...
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(B), 北海道大学, Coinvestigator not use grants, Competitive research funding, 17380014
  • クラスター根の形態形成に関与する遺伝子のRNAiによるスクリーニングと機能解析
    科学研究費補助金(萌芽研究)
    2005 - 2006
    山岸 真澄
    リン酸は窒素に次いで欠乏しやすい要素である。土壌中のリン酸の大部分は結合型で、植物が直接吸収できる可溶型リン酸の土壌溶液中の濃度はおよそ1pM程度、植物体内との濃度差は2千倍以上になる。ルーピンやヤマモガシ科の植物はクラスター根と呼ばれる特殊な根を作る。この根は1cm程度の短い側根を大量に密集させて発生することで表面積が通常の根の100倍以上になる。加えて酸性フォスファターゼや有機酸の分泌量が上昇しており、結合型のリン酸を溶かして吸収する能力が強い。クラスター根の形成はリン酸欠乏やオーキシンで促進されることが分かっているが、クラスター根を誘導する遺伝子やシグナル伝達系については分かっていない。白花ルーピンのクラスター根形成に関与する遺伝子を明らかにするために、クラスター根誘導のかかった根からcDNA libraryをすでに作成した。本研究ではこのlibraryからRNAi法(遺伝子ノックアウト法の一つ)で目的の遺伝子をスクリーニングして、その機能を解析する。まずRNAi法の開発に取り組んだ。作物でクラスター根を作るのは白花ルーピンだけであるが、白花ルーピンは形質転換できない。そこでpeanut stunt virus(PSV)を用いたRNAi法を検討した。この方法は形質転換を必要としない。光合成に必須な遺伝子であるPDSをこの方法でノックダウンしたところ、葉に白い斑が入り、か...
    文部科学省, 萌芽研究, 北海道大学, Principal investigator, Competitive research funding, 17658032
  • Molecular linkage map construction and mapping genes for useful traits in Asiatic hybrid lily
    Grants-in-Aid for Scientific Research(基盤研究(C))
    1999 - 2001
    Masumi YAMAGISHI
    To understand the genetic background of two floral anthocyanin pigmentation traits, anthocyanin pigmentation in the flower tepals and spot formation, in the Asiatic hybrid lily (2n=24), segregation of the two traits among 96 F_1 plants derived from a cross between commercial cultivars 'Montreux' and 'Connecticut King' were investigated. 'Montreux' has anthocyanin pigmentation in the tepals with many spots, and 'Connecticut King' has flowers with carotenoid pigmentation without spots. The F_1 plants with or without anthocyanin pigment in the tepals segregated with a 1 : 1 segregation ratio, ...
    Ministry of Education, Culture, Sports, Science and Technology, 基盤研究(C), 島根大学, Principal investigator, Competitive research funding, 11660031
  • 白山山系における高山植物の多様性の解明と遺伝子資源の保全法の確立に関する研究
    科学技術振興調査費 地域流動研究
    Apr. 1993 - Mar. 1997
    清水 建美
    科学技術庁, Competitive research funding
  • イネ葯培養に応答する遺伝子のマッピングと機能解析
    笹川科学研究助成
    1997
    山岸 真澄
    日本科学協会, Principal investigator, Competitive research funding
  • RAPD法によるユリ属の原種および交配種の識別
    科学研究費補助金(奨励研究(A))
    Apr. 1993 - Mar. 1994
    山岸 真澄
    ユリ属の種・種間交雑雑種・品種の遺伝的な解析に、Randomly Amplified Polymorphic DNA(RAPD)法を応用した。一般にRAPD法の場合、PCRのアニーリング温度は低いが、ユリ属でRAPD法を行う場合は、比較的高い、54℃がアニーリング温度として適当である事がわかった。10から18baseのランダムプライマーを76種類試したところ、18種類のプライマー(24%)が有効であった。これらのプライマーを用いて増幅されたDNAフィンガープリントの様相を示し、各々の種・雑種・品種の間で多型を検出し、これらを効率よく識別することができた。ユリ属のRAPD法は種や雑種や品種の鑑定に有用であると考えられた。PCRによって増幅された一部のDNA断片は、ユリ属の節(亜属)に特異的で、同じ節に属する種や、これらの種に由来する雑種に共通して増幅された。このような、節特異的なRAPDマーカーは6つ特定され、このうち1つはSinomartagon節に3つはLeucolirion b節に、1つはLeucolirion a節に、1つはArchelirion節に、それぞれ特異的であった。近年節間交雑雑種がいくつか作出されているが、7つの節間交雑雑種にRAPD法を用いたところ、各節間交雑雑種の二つの交配親がそれぞれ属する二つの節に特異的なマーカーが増幅された。この事は、節特異的なRA...
    文部科学省, 奨励研究(A), 石川県農業短期大学, Principal investigator, Competitive research funding, 05760036
  • ユリ属のDNAフィンガープリント法の開発
    科学研究費補助金(奨励研究(A))
    Apr. 1992 - Mar. 1993
    山岸 真澄
    文部科学省, Principal investigator, Competitive research funding